PubMed:25497339 JSONTXT

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    {"project":"sentences","denotations":[{"id":"TextSentencer_T1","span":{"begin":0,"end":149},"obj":"Sentence"},{"id":"TextSentencer_T2","span":{"begin":150,"end":250},"obj":"Sentence"},{"id":"TextSentencer_T3","span":{"begin":251,"end":340},"obj":"Sentence"},{"id":"TextSentencer_T4","span":{"begin":341,"end":532},"obj":"Sentence"},{"id":"TextSentencer_T5","span":{"begin":533,"end":750},"obj":"Sentence"},{"id":"TextSentencer_T6","span":{"begin":751,"end":896},"obj":"Sentence"},{"id":"TextSentencer_T7","span":{"begin":897,"end":1057},"obj":"Sentence"},{"id":"TextSentencer_T8","span":{"begin":1058,"end":1155},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":""}],"text":"Structural investigation of a uronic acid-containing polysaccharide from abalone by graded acid hydrolysis followed by PMP-HPLC-MSn and NMR analysis.\nA new strategy was applied to elucidate the structure of a polysaccharide from abalone gonad (AGSP). It was hydrolyzed by 0.05 M, 0.2 M, 0.5 M, and 2.0 M TFA at 100 \u00b0C for 1 h, sequentially. Every hydrolysate was ultrafiltrated (3000 Da) to collect oligo- and monosaccharides, and the final retentate was further hydrolyzed with 2.0 M TFA at 110 \u00b0C and 121 \u00b0C for 2 h, respectively. 1-Phenyl-3-methyl-5-pyrazolone (PMP) derivatization followed by HPLC-MSn analysis was applied to detect the sugar residues in these hydrolysates, which allowed proposing their location in the polysaccharide structure. The retentate after 0.5 M TFA hydrolysis was confirmed as the polysaccharide backbone, and it was further analyzed by 1D and 2D NMR spectroscopy. Thus, the structural elucidation of AGSP was accomplished, and it has a backbone of \u21924)-\u03b2-GlcA(1\u21922)-\u03b1-Man(1\u2192 repeating unit with Fuc, Xyl and Gal in the branch. The analytical strategy demonstrated was useful to characterize the structure of polysaccharides."}


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