Id |
Subject |
Object |
Predicate |
Lexical cue |
TextSentencer_T1 |
0-74 |
Sentence |
denotes |
Structural characterization of surface glycans from Clostridium difficile. |
TextSentencer_T2 |
75-262 |
Sentence |
denotes |
Whole-cell high-resolution magic angle spinning (HR-MAS) NMR was employed to survey the surface polysaccharides of a group of clinical and environmental isolates of Clostridium difficile. |
TextSentencer_T3 |
263-362 |
Sentence |
denotes |
Results indicated that a highly conserved surface polysaccharide profile among all strains studied. |
TextSentencer_T4 |
363-468 |
Sentence |
denotes |
Multiple additional peaks in the anomeric region were also observed which prompted further investigation. |
TextSentencer_T5 |
469-838 |
Sentence |
denotes |
Structural characterization of the isolated surface polysaccharides from two strains confirmed the presence of the conserved water soluble polysaccharide originally described by Ganeshapillai et al. which was composed of a hexaglycosyl phosphate repeat consisting of [→6)-β-D-Glcp-(1-3)-β-D-GalpNAc-(1-4)-α-D-Glcp-(1-4)-[β-D-Glcp(1-3]-β-D-GalpNAc-(1-3)-α-D-Manp-(1-P→]. |
TextSentencer_T6 |
839-1003 |
Sentence |
denotes |
In addition, analysis of phenol soluble polysaccharides revealed a similarly conserved lipoteichoic acid (LTA) which could be detected on whole cells by HR-MAS NMR. |
TextSentencer_T7 |
1004-1206 |
Sentence |
denotes |
Conventional NMR and mass spectrometry analysis indicated that the structure of this LTA consisted of the repeat unit [→6)-α-D-GlcpNAc-(1-3)-[→P-6]-α-D-GlcpNAc-(1-2)-D-GroA] where GroA is glyceric acid. |
TextSentencer_T8 |
1207-1313 |
Sentence |
denotes |
The repeating units were linked by a phosphodiester bridge between C-6 of the two GlcNAc residues (6-P-6). |
TextSentencer_T9 |
1314-1401 |
Sentence |
denotes |
A minor component consisted of GlcpN-(1-3) instead of GlcpNAc-(1-3) in the repeat unit. |
TextSentencer_T10 |
1402-1566 |
Sentence |
denotes |
Through a 6-6 phosphodiester bridge this polymer was linked to →6)-β-D-Glcp-(1-6)-β-D-Glcp-(1-6)-β-D-Glcp-(1-1)-Gro, with glycerol (Gro) substituted by fatty acids. |
TextSentencer_T11 |
1567-1762 |
Sentence |
denotes |
This is the first report of the utility of HR-MAS NMR in the examination of surface carbohydrates of Gram positive bacteria and identification of a novel LTA structure from Clostridium difficile. |
T1 |
0-74 |
Sentence |
denotes |
Structural characterization of surface glycans from Clostridium difficile. |
T2 |
75-262 |
Sentence |
denotes |
Whole-cell high-resolution magic angle spinning (HR-MAS) NMR was employed to survey the surface polysaccharides of a group of clinical and environmental isolates of Clostridium difficile. |
T3 |
263-362 |
Sentence |
denotes |
Results indicated that a highly conserved surface polysaccharide profile among all strains studied. |
T4 |
363-468 |
Sentence |
denotes |
Multiple additional peaks in the anomeric region were also observed which prompted further investigation. |
T5 |
469-838 |
Sentence |
denotes |
Structural characterization of the isolated surface polysaccharides from two strains confirmed the presence of the conserved water soluble polysaccharide originally described by Ganeshapillai et al. which was composed of a hexaglycosyl phosphate repeat consisting of [→6)-β-D-Glcp-(1-3)-β-D-GalpNAc-(1-4)-α-D-Glcp-(1-4)-[β-D-Glcp(1-3]-β-D-GalpNAc-(1-3)-α-D-Manp-(1-P→]. |
T6 |
839-1003 |
Sentence |
denotes |
In addition, analysis of phenol soluble polysaccharides revealed a similarly conserved lipoteichoic acid (LTA) which could be detected on whole cells by HR-MAS NMR. |
T7 |
1004-1206 |
Sentence |
denotes |
Conventional NMR and mass spectrometry analysis indicated that the structure of this LTA consisted of the repeat unit [→6)-α-D-GlcpNAc-(1-3)-[→P-6]-α-D-GlcpNAc-(1-2)-D-GroA] where GroA is glyceric acid. |
T8 |
1207-1313 |
Sentence |
denotes |
The repeating units were linked by a phosphodiester bridge between C-6 of the two GlcNAc residues (6-P-6). |
T9 |
1314-1401 |
Sentence |
denotes |
A minor component consisted of GlcpN-(1-3) instead of GlcpNAc-(1-3) in the repeat unit. |
T10 |
1402-1566 |
Sentence |
denotes |
Through a 6-6 phosphodiester bridge this polymer was linked to →6)-β-D-Glcp-(1-6)-β-D-Glcp-(1-6)-β-D-Glcp-(1-1)-Gro, with glycerol (Gro) substituted by fatty acids. |
T11 |
1567-1762 |
Sentence |
denotes |
This is the first report of the utility of HR-MAS NMR in the examination of surface carbohydrates of Gram positive bacteria and identification of a novel LTA structure from Clostridium difficile. |