| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T1 |
0-148 |
Sentence |
denotes |
A Novel Basal Body Protein That Is a Polo-like Kinase Substrate Is Required for Basal Body Segregation and Flagellum Adhesion in Trypanosoma brucei. |
| T2 |
149-284 |
Sentence |
denotes |
The Polo-like kinase (PLK) in Trypanosoma brucei plays multiple roles in basal body segregation, flagellum attachment, and cytokinesis. |
| T3 |
285-393 |
Sentence |
denotes |
However, the mechanistic role of TbPLK remains elusive, mainly because most of its substrates are not known. |
| T4 |
394-480 |
Sentence |
denotes |
Here, we report a new substrate of TbPLK, SPBB1, and its essential roles in T. brucei. |
| T5 |
481-576 |
Sentence |
denotes |
SPBB1 was identified through yeast two-hybrid screening with the kinase-dead TbPLK as the bait. |
| T6 |
577-663 |
Sentence |
denotes |
It interacts with TbPLK in vitro and in vivo, and is phosphorylated by TbPLK in vitro. |
| T7 |
664-836 |
Sentence |
denotes |
SPBB1 localizes to both the mature basal body and the probasal body throughout the cell cycle, and co-localizes with TbPLK at the basal body during early cell cycle stages. |
| T8 |
837-1031 |
Sentence |
denotes |
RNAi against SPBB1 in procyclic trypanosomes inhibited basal body segregation, disrupted the new flagellum attachment zone filament, detached the new flagellum, and caused defective cytokinesis. |
| T9 |
1032-1185 |
Sentence |
denotes |
Moreover, RNAi of SPBB1 confined TbPLK at the basal body and the bilobe structure, resulting in constitutive phosphorylation of TbCentrin2 at the bilobe. |
| T10 |
1186-1422 |
Sentence |
denotes |
Altogether, these results identified a basal body protein as a TbPLK substrate and its essential role in promoting basal body segregation and flagellum attachment zone filament assembly for flagellum adhesion and cytokinesis initiation. |
