> top > projects > sentences > docs > PubMed:22042767 > annotations
sentences Home  

PubMed:22042767 JSONTXT 48 Projects

Annnotations TAB TSV DIC JSON TextAE

Id Subject Object Predicate Lexical cue
TextSentencer_T1 0-103 Sentence denotes A new glycoengineered insect cell line with an inducibly mammalianized protein N-glycosylation pathway.
T1 0-103 Sentence denotes A new glycoengineered insect cell line with an inducibly mammalianized protein N-glycosylation pathway.
T1 0-103 Sentence denotes A new glycoengineered insect cell line with an inducibly mammalianized protein N-glycosylation pathway.
TextSentencer_T2 104-244 Sentence denotes The inability to produce recombinant glycoproteins with authentic N-glycans is a limitation of many heterologous protein expression systems.
T2 104-244 Sentence denotes The inability to produce recombinant glycoproteins with authentic N-glycans is a limitation of many heterologous protein expression systems.
T2 104-244 Sentence denotes The inability to produce recombinant glycoproteins with authentic N-glycans is a limitation of many heterologous protein expression systems.
TextSentencer_T3 245-496 Sentence denotes In the baculovirus-insect cell system, this limitation has been addressed by glycoengineering insect cell lines with mammalian genes encoding protein N-glycosylation functions ("glycogenes") under the transcriptional control of constitutive promoters.
T3 245-496 Sentence denotes In the baculovirus-insect cell system, this limitation has been addressed by glycoengineering insect cell lines with mammalian genes encoding protein N-glycosylation functions ("glycogenes") under the transcriptional control of constitutive promoters.
T3 245-496 Sentence denotes In the baculovirus-insect cell system, this limitation has been addressed by glycoengineering insect cell lines with mammalian genes encoding protein N-glycosylation functions ("glycogenes") under the transcriptional control of constitutive promoters.
TextSentencer_T4 497-709 Sentence denotes However, a potential problem with this approach is that the metabolic load imposed by the expression of multiple transgenes could adversely impact the growth and/or stability of glycoengineered insect cell lines.
T4 497-709 Sentence denotes However, a potential problem with this approach is that the metabolic load imposed by the expression of multiple transgenes could adversely impact the growth and/or stability of glycoengineered insect cell lines.
T4 497-709 Sentence denotes However, a potential problem with this approach is that the metabolic load imposed by the expression of multiple transgenes could adversely impact the growth and/or stability of glycoengineered insect cell lines.
TextSentencer_T5 710-835 Sentence denotes Thus, we created a new transgenic insect cell line (SfSWT-5) with an inducibly mammalianized protein N-glycosylation pathway.
T5 710-835 Sentence denotes Thus, we created a new transgenic insect cell line (SfSWT-5) with an inducibly mammalianized protein N-glycosylation pathway.
T5 710-835 Sentence denotes Thus, we created a new transgenic insect cell line (SfSWT-5) with an inducibly mammalianized protein N-glycosylation pathway.
TextSentencer_T6 836-965 Sentence denotes Expression of all six glycogenes was induced when uninfected SfSWT-5 cells were cultured in growth medium containing doxycycline.
T6 836-965 Sentence denotes Expression of all six glycogenes was induced when uninfected SfSWT-5 cells were cultured in growth medium containing doxycycline.
T6 836-965 Sentence denotes Expression of all six glycogenes was induced when uninfected SfSWT-5 cells were cultured in growth medium containing doxycycline.
TextSentencer_T7 966-1104 Sentence denotes Higher levels of expression and induction were observed when SfSWT-5 cells were cultured with doxycycline and infected with a baculovirus.
T7 966-1104 Sentence denotes Higher levels of expression and induction were observed when SfSWT-5 cells were cultured with doxycycline and infected with a baculovirus.
T7 966-1104 Sentence denotes Higher levels of expression and induction were observed when SfSWT-5 cells were cultured with doxycycline and infected with a baculovirus.
TextSentencer_T8 1105-1242 Sentence denotes Interestingly, there were no major differences in the short-term growth properties of SfSWT-5 cells cultured with or without doxycycline.
T8 1105-1242 Sentence denotes Interestingly, there were no major differences in the short-term growth properties of SfSWT-5 cells cultured with or without doxycycline.
T8 1105-1242 Sentence denotes Interestingly, there were no major differences in the short-term growth properties of SfSWT-5 cells cultured with or without doxycycline.
TextSentencer_T9 1243-1406 Sentence denotes Furthermore, there were no major differences in the phenotypic stability of these cells after continuous culture for over 300 passages with or without doxycycline.
T9 1243-1406 Sentence denotes Furthermore, there were no major differences in the phenotypic stability of these cells after continuous culture for over 300 passages with or without doxycycline.
T9 1243-1406 Sentence denotes Furthermore, there were no major differences in the phenotypic stability of these cells after continuous culture for over 300 passages with or without doxycycline.
TextSentencer_T10 1407-1644 Sentence denotes Baculovirus-infected Sf9 and SfSWT-5 cells produced about the same amounts of a model recombinant glycoprotein, but only the latter sialylated this product and sialylation was more pronounced when the cells were treated with doxycycline.
T10 1407-1644 Sentence denotes Baculovirus-infected Sf9 and SfSWT-5 cells produced about the same amounts of a model recombinant glycoprotein, but only the latter sialylated this product and sialylation was more pronounced when the cells were treated with doxycycline.
T10 1407-1644 Sentence denotes Baculovirus-infected Sf9 and SfSWT-5 cells produced about the same amounts of a model recombinant glycoprotein, but only the latter sialylated this product and sialylation was more pronounced when the cells were treated with doxycycline.
TextSentencer_T11 1645-1914 Sentence denotes In summary, this is the first report of a lower eukaryotic system with an inducibly mammalianized protein N-glycosylation pathway and the first to examine how the presumed metabolic load imposed by multiple transgene expression impacts insect cell growth and stability.
T11 1645-1914 Sentence denotes In summary, this is the first report of a lower eukaryotic system with an inducibly mammalianized protein N-glycosylation pathway and the first to examine how the presumed metabolic load imposed by multiple transgene expression impacts insect cell growth and stability.
T11 1645-1914 Sentence denotes In summary, this is the first report of a lower eukaryotic system with an inducibly mammalianized protein N-glycosylation pathway and the first to examine how the presumed metabolic load imposed by multiple transgene expression impacts insect cell growth and stability.