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PMC:3342329 / 32522-33684 JSONTXT 7 Projects

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Id Subject Object Predicate Lexical cue
T15701 0-1 DT denotes A
T15702 2-5 NN denotes gel
T15703 6-21 NN denotes electromobility
T15704 22-27 NN denotes shift
T15705 28-33 NN denotes assay
T15706 34-35 -LRB- denotes (
T15707 35-39 NN denotes EMSA
T15708 39-40 -RRB- denotes )
T15709 41-44 VB denotes was
T15710 45-54 VB denotes performed
T15711 55-64 VB denotes according
T15712 65-67 TO denotes to
T15713 68-71 DT denotes the
T15714 72-86 NN denotes manufacturer’s
T15715 87-102 NN denotes recommendations
T15716 103-104 -LRB- denotes (
T15717 104-111 NNP denotes Promega
T15718 111-112 -COMMA- denotes ,
T15719 113-120 NNP denotes Madison
T15720 120-121 -COMMA- denotes ,
T15721 122-124 NNP denotes WI
T15722 124-125 -RRB- denotes )
T15723 127-130 DT denotes The
T15724 131-136 NN denotes tumor
T15725 137-144 NN denotes tissues
T15726 145-149 VB denotes were
T15727 150-157 RB denotes briefly
T15728 158-169 VB denotes homogenized
T15729 170-172 IN denotes in
T15730 173-176 CD denotes 200
T15731 177-179 NN denotes µl
T15732 180-182 IN denotes of
T15733 183-191 NN denotes solution
T15734 192-193 NN denotes A
T15735 194-195 -LRB- denotes (
T15736 195-197 CD denotes 10
T15737 198-200 NN denotes mM
T15738 201-206 NN denotes HEPES
T15739 207-208 -LRB- denotes [
T15740 208-210 NN denotes pH
T15741 211-214 CD denotes 7.9
T15742 214-215 -RRB- denotes ]
T15743 215-216 -COMMA- denotes ,
T15744 217-220 CD denotes 1.5
T15745 221-223 NN denotes mM
T15746 224-229 NN denotes MgCl2
T15747 229-230 -COMMA- denotes ,
T15748 231-233 CD denotes 10
T15749 234-236 NN denotes mM
T15750 237-240 NN denotes KCl
T15751 240-241 -COMMA- denotes ,
T15752 242-245 CD denotes 0.5
T15753 246-248 NN denotes mM
T15754 249-263 NN denotes dithiothreitol
T15755 263-264 -COMMA- denotes ,
T15756 265-268 CD denotes 0.2
T15757 269-271 NN denotes mM
T15758 272-300 NN denotes phenylmethylsulfonylfluoride
T15759 300-301 -RRB- denotes )
T15760 301-302 -COMMA- denotes ,
T15761 303-311 VB denotes vortexed
T15762 312-322 RB denotes vigorously
T15763 322-323 -COMMA- denotes ,
T15764 324-333 VB denotes incubated
T15765 334-336 IN denotes on
T15766 337-340 NN denotes ice
T15767 341-344 IN denotes for
T15768 345-347 CD denotes 10
T15769 348-351 NN denotes min
T15770 351-352 -COMMA- denotes ,
T15771 353-356 CC denotes and
T15772 357-361 RB denotes then
T15773 362-373 VB denotes centrifuged
T15774 374-376 IN denotes at
T15775 377-382 CD denotes 15000
T15776 383-386 NN denotes rpm
T15777 387-390 IN denotes for
T15778 391-393 CD denotes 15
T15779 394-397 NN denotes min
T15780 399-402 DT denotes The
T15781 403-411 VB denotes pelleted
T15782 412-418 NN denotes nuclei
T15783 419-423 VB denotes were
T15784 424-435 VB denotes resuspended
T15785 436-438 IN denotes in
T15786 439-447 NN denotes solution
T15787 448-449 NN denotes C
T15788 450-451 -LRB- denotes (
T15789 451-459 NN denotes solution
T15790 460-461 NN denotes A
T15791 462-474 VB denotes supplemented
T15792 475-479 IN denotes with
T15793 480-483 CD denotes 420
T15794 484-486 NN denotes mM
T15795 487-491 NN denotes NaCl
T15796 492-495 CC denotes and
T15797 496-498 CD denotes 20
T15798 498-499 NN denotes %
T15799 500-508 NN denotes glycerol
T15800 508-509 -RRB- denotes )
T15801 509-510 -COMMA- denotes ,
T15802 511-514 CC denotes and
T15803 515-524 VB denotes incubated
T15804 525-527 IN denotes on
T15805 528-531 NN denotes ice
T15806 532-535 IN denotes for
T15807 536-538 CD denotes 20
T15808 539-542 NN denotes min
T15809 544-547 DT denotes The
T15810 548-559 JJ denotes resuspended
T15811 560-566 NN denotes pellet
T15812 567-570 VB denotes was
T15813 571-582 VB denotes centrifuged
T15814 583-585 IN denotes at
T15815 586-591 CD denotes 15000
T15816 592-595 NN denotes rpm
T15817 596-599 IN denotes for
T15818 600-602 CD denotes 15
T15819 603-606 NN denotes min
T15820 606-607 -COMMA- denotes ,
T15821 608-611 CC denotes and
T15822 612-615 DT denotes the
T15823 616-625 VB denotes resulting
T15824 626-633 JJ denotes nuclear
T15825 634-642 NN denotes extracts
T15826 643-654 NN denotes supernatant
T15827 655-659 VB denotes were
T15828 660-669 VB denotes collected
T15829 670-672 IN denotes in
T15830 673-674 DT denotes a
T15831 675-682 JJ denotes chilled
T15832 683-692 NNP denotes Eppendorf
T15833 693-697 NN denotes tube
T15834 699-708 NNP denotes Consensus
T15835 709-725 NN denotes oligonucleotides
T15836 726-730 VB denotes were
T15837 731-742 VB denotes end-labeled
T15838 743-748 VB denotes using
T15839 749-751 NN denotes T4
T15840 752-766 NN denotes polynucleotide
T15841 767-773 NN denotes kinase
T15842 774-777 CC denotes and
T15843 778-779 -LRB- denotes [
T15844 779-782 NN denotes P32
T15845 782-783 -RRB- denotes ]
T15846 783-787 NN denotes -ATP
T15847 788-791 IN denotes for
T15848 792-794 CD denotes 10
T15849 795-798 NN denotes min
T15850 799-801 IN denotes at
T15851 802-807 NNP denotes 37°C.
T15852 808-811 DT denotes The
T15853 812-815 NN denotes gel
T15854 816-821 NN denotes shift
T15855 822-831 NN denotes reactions
T15856 832-836 VB denotes were
T15857 837-846 VB denotes assembled
T15858 847-850 CC denotes and
T15859 851-860 VB denotes incubated
T15860 861-863 IN denotes at
T15861 864-868 NN denotes room
T15862 869-880 NN denotes temperature
T15863 882-894 RB denotes Subsequently
T15864 894-895 -COMMA- denotes ,
T15865 896-897 CD denotes 1
T15866 898-900 NN denotes ml
T15867 901-903 IN denotes of
T15868 904-907 NN denotes gel
T15869 908-915 NN denotes loading
T15870 916-922 NN denotes buffer
T15871 923-926 VB denotes was
T15872 927-932 VB denotes added
T15873 933-935 TO denotes to
T15874 936-940 DT denotes each
T15875 941-949 NN denotes reaction
T15876 950-953 CC denotes and
T15877 954-960 VB denotes loaded
T15878 961-965 IN denotes onto
T15879 966-967 DT denotes a
T15880 968-969 CD denotes 6
T15881 969-970 NN denotes %
T15882 971-987 JJ denotes non-denaturating
T15883 988-991 NN denotes gel
T15884 993-996 DT denotes The
T15885 997-1000 NN denotes gel
T15886 1001-1004 VB denotes was
T15887 1005-1014 VB denotes subjected
T15888 1015-1017 TO denotes to
T15889 1018-1033 NN denotes electrophoresis
T15890 1034-1039 IN denotes until
T15891 1040-1043 DT denotes the
T15892 1044-1047 NN denotes dye
T15893 1048-1051 VB denotes was
T15894 1052-1063 NN denotes four-fifths
T15895 1064-1066 IN denotes of
T15896 1067-1070 DT denotes the
T15897 1071-1074 NN denotes way
T15898 1075-1079 IN denotes down
T15899 1080-1083 DT denotes the
T15900 1084-1087 NN denotes gel
T15901 1089-1092 DT denotes The
T15902 1093-1096 NN denotes gel
T15903 1097-1100 VB denotes was
T15904 1101-1106 VB denotes dried
T15905 1107-1110 IN denotes for
T15906 1111-1112 CD denotes 1
T15907 1113-1114 NN denotes h
T15908 1115-1117 IN denotes at
T15909 1118-1122 NN denotes 80°C
T15910 1123-1126 CC denotes and
T15911 1127-1134 VB denotes exposed
T15912 1135-1137 TO denotes to
T15913 1138-1142 VB denotes film
T15914 1143-1152 NN denotes overnight
T15915 1153-1155 IN denotes at
T15916 1156-1161 NN denotes -70°C
R11128 T15705 T15701 arg1Of assay,A
R11129 T15705 T15702 arg1Of assay,gel
R11130 T15705 T15703 arg1Of assay,electromobility
R11131 T15705 T15704 arg1Of assay,shift
R11132 T15705 T15706 arg1Of assay,(
R11133 T15705 T15709 arg1Of assay,was
R11134 T15705 T15710 arg2Of assay,performed
R11135 T15707 T15706 arg2Of EMSA,(
R11136 T15708 T15706 arg3Of ),(
R11137 T15710 T15709 arg2Of performed,was
R11138 T15710 T15711 arg1Of performed,according
R11139 T15710 T15716 arg1Of performed,(
R11140 T15712 T15711 arg2Of to,according
R11141 T15715 T15712 arg2Of recommendations,to
R11142 T15715 T15713 arg1Of recommendations,the
R11143 T15715 T15714 arg1Of recommendations,manufacturer’s
R11144 T15717 T15718 arg1Of Promega,","
R11145 T15718 T15716 arg2Of ",",(
R11146 T15718 T15720 arg1Of ",",","
R11147 T15719 T15718 arg2Of Madison,","
R11148 T15721 T15720 arg2Of WI,","
R11149 T15722 T15716 arg3Of ),(
R11150 T15725 T15723 arg1Of tissues,The
R11151 T15725 T15724 arg1Of tissues,tumor
R11152 T15725 T15726 arg1Of tissues,were
R11153 T15725 T15728 arg2Of tissues,homogenized
R11154 T15725 T15761 arg1Of tissues,vortexed
R11155 T15725 T15764 arg1Of tissues,incubated
R11156 T15725 T15773 arg1Of tissues,centrifuged
R11157 T15728 T15726 arg2Of homogenized,were
R11158 T15728 T15727 arg1Of homogenized,briefly
R11159 T15728 T15729 arg1Of homogenized,in
R11160 T15728 T15760 arg1Of homogenized,","
R11161 T15731 T15729 arg2Of µl,in
R11162 T15731 T15730 arg1Of µl,200
R11163 T15731 T15732 arg1Of µl,of
R11164 T15731 T15735 arg1Of µl,(
R11165 T15734 T15732 arg2Of A,of
R11166 T15734 T15733 arg1Of A,solution
R11167 T15736 T15737 arg1Of 10,mM
R11168 T15738 T15736 arg1Of HEPES,10
R11169 T15738 T15739 arg1Of HEPES,[
R11170 T15738 T15743 arg1Of HEPES,","
R11171 T15740 T15739 arg2Of pH,[
R11172 T15740 T15741 arg1Of pH,7.9
R11173 T15742 T15739 arg3Of ],[
R11174 T15743 T15747 arg1Of ",",","
R11175 T15744 T15745 arg1Of 1.5,mM
R11176 T15746 T15743 arg2Of MgCl2,","
R11177 T15746 T15744 arg1Of MgCl2,1.5
R11178 T15747 T15751 arg1Of ",",","
R11179 T15748 T15749 arg1Of 10,mM
R11180 T15750 T15747 arg2Of KCl,","
R11181 T15750 T15748 arg1Of KCl,10
R11182 T15751 T15735 arg2Of ",",(
R11183 T15751 T15755 arg1Of ",",","
R11184 T15752 T15753 arg1Of 0.5,mM
R11185 T15754 T15751 arg2Of dithiothreitol,","
R11186 T15754 T15752 arg1Of dithiothreitol,0.5
R11187 T15756 T15757 arg1Of 0.2,mM
R11188 T15758 T15755 arg2Of phenylmethylsulfonylfluoride,","
R11189 T15758 T15756 arg1Of phenylmethylsulfonylfluoride,0.2
R11190 T15759 T15735 arg3Of ),(
R11191 T15760 T15763 arg1Of ",",","
R11192 T15761 T15760 arg2Of vortexed,","
R11193 T15761 T15762 arg1Of vortexed,vigorously
R11194 T15763 T15771 arg1Of ",",and
R11195 T15764 T15763 arg2Of incubated,","
R11196 T15764 T15765 arg1Of incubated,on
R11197 T15764 T15767 arg1Of incubated,for
R11198 T15766 T15765 arg2Of ice,on
R11199 T15769 T15767 arg2Of min,for
R11200 T15769 T15768 arg1Of min,10
R11201 T15771 T15770 arg1Of and,","
R11202 T15773 T15771 arg2Of centrifuged,and
R11203 T15773 T15772 arg1Of centrifuged,then
R11204 T15773 T15774 arg1Of centrifuged,at
R11205 T15773 T15777 arg1Of centrifuged,for
R11206 T15776 T15774 arg2Of rpm,at
R11207 T15776 T15775 arg1Of rpm,15000
R11208 T15779 T15777 arg2Of min,for
R11209 T15779 T15778 arg1Of min,15
R11210 T15782 T15780 arg1Of nuclei,The
R11211 T15782 T15781 arg2Of nuclei,pelleted
R11212 T15782 T15783 arg1Of nuclei,were
R11213 T15782 T15784 arg2Of nuclei,resuspended
R11214 T15782 T15803 arg2Of nuclei,incubated
R11215 T15784 T15785 arg1Of resuspended,in
R11216 T15784 T15802 arg1Of resuspended,and
R11217 T15787 T15785 arg2Of C,in
R11218 T15787 T15786 arg1Of C,solution
R11219 T15787 T15788 arg1Of C,(
R11220 T15790 T15788 arg2Of A,(
R11221 T15790 T15789 arg1Of A,solution
R11222 T15790 T15791 arg2Of A,supplemented
R11223 T15791 T15792 arg1Of supplemented,with
R11224 T15793 T15794 arg1Of 420,mM
R11225 T15795 T15793 arg1Of NaCl,420
R11226 T15795 T15796 arg1Of NaCl,and
R11227 T15796 T15792 arg2Of and,with
R11228 T15797 T15798 arg1Of 20,%
R11229 T15799 T15796 arg2Of glycerol,and
R11230 T15799 T15797 arg1Of glycerol,20
R11231 T15800 T15788 arg3Of ),(
R11232 T15802 T15783 arg2Of and,were
R11233 T15802 T15801 arg1Of and,","
R11234 T15803 T15802 arg2Of incubated,and
R11235 T15803 T15804 arg1Of incubated,on
R11236 T15803 T15806 arg1Of incubated,for
R11237 T15805 T15804 arg2Of ice,on
R11238 T15808 T15806 arg2Of min,for
R11239 T15808 T15807 arg1Of min,20
R11240 T15811 T15809 arg1Of pellet,The
R11241 T15811 T15810 arg1Of pellet,resuspended
R11242 T15811 T15812 arg1Of pellet,was
R11243 T15811 T15813 arg2Of pellet,centrifuged
R11244 T15813 T15812 arg2Of centrifuged,was
R11245 T15813 T15814 arg1Of centrifuged,at
R11246 T15813 T15817 arg1Of centrifuged,for
R11247 T15813 T15821 arg1Of centrifuged,and
R11248 T15816 T15814 arg2Of rpm,at
R11249 T15816 T15815 arg1Of rpm,15000
R11250 T15819 T15817 arg2Of min,for
R11251 T15819 T15818 arg1Of min,15
R11252 T15821 T15820 arg1Of and,","
R11253 T15826 T15822 arg1Of supernatant,the
R11254 T15826 T15823 arg1Of supernatant,resulting
R11255 T15826 T15824 arg1Of supernatant,nuclear
R11256 T15826 T15825 arg1Of supernatant,extracts
R11257 T15826 T15827 arg1Of supernatant,were
R11258 T15826 T15828 arg2Of supernatant,collected
R11259 T15828 T15821 arg2Of collected,and
R11260 T15828 T15827 arg2Of collected,were
R11261 T15828 T15829 arg1Of collected,in
R11262 T15833 T15829 arg2Of tube,in
R11263 T15833 T15830 arg1Of tube,a
R11264 T15833 T15831 arg1Of tube,chilled
R11265 T15833 T15832 arg1Of tube,Eppendorf
R11266 T15835 T15834 arg1Of oligonucleotides,Consensus
R11267 T15835 T15836 arg1Of oligonucleotides,were
R11268 T15835 T15837 arg2Of oligonucleotides,end-labeled
R11269 T15835 T15859 arg2Of oligonucleotides,incubated
R11270 T15837 T15858 arg1Of end-labeled,and
R11271 T15838 T15837 arg3Of using,end-labeled
R11272 T15838 T15847 arg1Of using,for
R11273 T15841 T15839 arg1Of kinase,T4
R11274 T15841 T15840 arg1Of kinase,polynucleotide
R11275 T15841 T15842 arg1Of kinase,and
R11276 T15842 T15838 arg2Of and,using
R11277 T15844 T15843 arg2Of P32,[
R11278 T15845 T15843 arg3Of ],[
R11279 T15846 T15842 arg2Of -ATP,and
R11280 T15846 T15843 arg1Of -ATP,[
R11281 T15849 T15847 arg2Of min,for
R11282 T15849 T15848 arg1Of min,10
R11283 T15849 T15850 arg1Of min,at
R11284 T15851 T15850 arg2Of 37°C.,at
R11285 T15851 T15856 modOf 37°C.,were
R11286 T15855 T15852 arg1Of reactions,The
R11287 T15855 T15853 arg1Of reactions,gel
R11288 T15855 T15854 arg1Of reactions,shift
R11289 T15855 T15856 arg1Of reactions,were
R11290 T15855 T15857 arg2Of reactions,assembled
R11291 T15857 T15856 arg2Of assembled,were
R11292 T15858 T15836 arg2Of and,were
R11293 T15859 T15858 arg2Of incubated,and
R11294 T15859 T15860 arg1Of incubated,at
R11295 T15862 T15860 arg2Of temperature,at
R11296 T15862 T15861 arg1Of temperature,room
R11297 T15866 T15865 arg1Of ml,1
R11298 T15866 T15867 arg1Of ml,of
R11299 T15866 T15871 arg1Of ml,was
R11300 T15866 T15872 arg2Of ml,added
R11301 T15866 T15877 arg2Of ml,loaded
R11302 T15870 T15867 arg2Of buffer,of
R11303 T15870 T15868 arg1Of buffer,gel
R11304 T15870 T15869 arg1Of buffer,loading
R11305 T15872 T15873 arg1Of added,to
R11306 T15872 T15876 arg1Of added,and
R11307 T15875 T15873 arg2Of reaction,to
R11308 T15875 T15874 arg1Of reaction,each
R11309 T15876 T15863 arg1Of and,Subsequently
R11310 T15876 T15864 arg1Of and,","
R11311 T15876 T15871 arg2Of and,was
R11312 T15877 T15876 arg2Of loaded,and
R11313 T15877 T15878 arg1Of loaded,onto
R11314 T15880 T15881 arg1Of 6,%
R11315 T15883 T15878 arg2Of gel,onto
R11316 T15883 T15879 arg1Of gel,a
R11317 T15883 T15880 arg1Of gel,6
R11318 T15883 T15882 arg1Of gel,non-denaturating
R11319 T15885 T15884 arg1Of gel,The
R11320 T15885 T15886 arg1Of gel,was
R11321 T15885 T15887 arg2Of gel,subjected
R11322 T15887 T15890 arg1Of subjected,until
R11323 T15887 T15886 arg2Of subjected,was
R11324 T15887 T15888 arg1Of subjected,to
R11325 T15889 T15888 arg2Of electrophoresis,to
R11326 T15892 T15891 arg1Of dye,the
R11327 T15892 T15893 arg1Of dye,was
R11328 T15893 T15890 arg2Of was,until
R11329 T15894 T15893 arg2Of four-fifths,was
R11330 T15894 T15895 arg1Of four-fifths,of
R11331 T15897 T15895 arg2Of way,of
R11332 T15897 T15896 arg1Of way,the
R11333 T15897 T15898 arg1Of way,down
R11334 T15900 T15898 arg2Of gel,down
R11335 T15900 T15899 arg1Of gel,the
R11336 T15902 T15901 arg1Of gel,The
R11337 T15902 T15903 arg1Of gel,was
R11338 T15902 T15904 arg2Of gel,dried
R11339 T15902 T15911 arg2Of gel,exposed
R11340 T15904 T15905 arg1Of dried,for
R11341 T15904 T15908 arg1Of dried,at
R11342 T15904 T15910 arg1Of dried,and
R11343 T15907 T15905 arg2Of h,for
R11344 T15907 T15906 arg1Of h,1
R11345 T15909 T15908 arg2Of 80°C,at
R11346 T15910 T15903 arg2Of and,was
R11347 T15911 T15910 arg2Of exposed,and
R11348 T15911 T15912 modOf exposed,to
R11349 T15913 T15912 arg1Of film,to
R11350 T15913 T15915 arg1Of film,at
R11351 T15914 T15913 arg2Of overnight,film
R11352 T15916 T15915 arg2Of -70°C,at