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PMC:2817507 / 8457-9349 JSONTXT 22 Projects

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Id Subject Object Predicate Lexical cue
T4034 0-3 CD denotes 2.6
T4035 5-20 JJ denotes Electrophoretic
T4036 21-29 NN denotes Mobility
T4037 30-35 NN denotes Shift
T4038 36-41 NN denotes Assay
T4039 42-43 -LRB- denotes (
T4040 43-47 NN denotes EMSA
T4041 47-48 -RRB- denotes )
T4042 49-56 JJ denotes Nuclear
T4043 57-65 NN denotes proteins
T4044 66-70 VB denotes were
T4045 71-79 VB denotes isolated
T4046 80-84 IN denotes from
T4047 85-90 NN denotes brain
T4048 91-97 NN denotes tissue
T4049 99-106 RB denotes Briefly
T4050 106-107 -COMMA- denotes ,
T4051 108-113 IN denotes after
T4052 114-121 NN denotes removal
T4053 122-124 IN denotes of
T4054 125-136 NN denotes supernatant
T4055 137-138 -LRB- denotes (
T4056 138-149 JJ denotes cytoplasmic
T4057 150-157 NN denotes extract
T4058 157-158 -RRB- denotes )
T4059 158-159 -COMMA- denotes ,
T4060 160-163 DT denotes the
T4061 164-171 NN denotes pellets
T4062 172-176 VB denotes were
T4063 177-188 VB denotes resuspended
T4064 189-191 IN denotes in
T4065 192-199 JJ denotes nuclear
T4066 200-210 NN denotes extraction
T4067 211-218 NN denotes reagent
T4068 220-223 DT denotes The
T4069 224-229 NN denotes tubes
T4070 230-240 VB denotes containing
T4071 241-248 NN denotes pellets
T4072 249-253 VB denotes were
T4073 254-257 VB denotes put
T4074 258-260 IN denotes on
T4075 261-267 NN denotes vortex
T4076 268-271 IN denotes for
T4077 272-274 CD denotes 15
T4078 275-282 NN denotes seconds
T4079 283-288 DT denotes every
T4080 289-291 CD denotes 10
T4081 292-299 NN denotes minutes
T4082 299-300 -COMMA- denotes ,
T4083 301-304 IN denotes for
T4084 305-306 DT denotes a
T4085 307-312 NN denotes total
T4086 313-315 IN denotes of
T4087 316-318 CD denotes 40
T4088 319-326 NN denotes minutes
T4089 328-331 DT denotes The
T4090 332-337 NN denotes tubes
T4091 338-342 VB denotes were
T4092 343-354 VB denotes centrifuged
T4093 355-357 IN denotes at
T4094 358-365 JJ denotes maximum
T4095 366-371 NN denotes speed
T4096 372-373 -LRB- denotes (
T4097 373-380 CD denotes ~16,000
T4098 381-384 NN denotes × g
T4099 384-385 -RRB- denotes )
T4100 386-388 IN denotes in
T4101 389-390 DT denotes a
T4102 391-396 NN denotes micro
T4103 397-407 NN denotes centrifuge
T4104 408-411 IN denotes for
T4105 412-414 CD denotes 10
T4106 415-422 NN denotes minutes
T4107 424-427 DT denotes The
T4108 428-440 NN denotes supernatants
T4109 441-442 -LRB- denotes (
T4110 442-449 JJ denotes nuclear
T4111 450-457 NN denotes extract
T4112 457-458 -RRB- denotes )
T4113 459-463 VB denotes were
T4114 464-475 VB denotes transferred
T4115 476-478 TO denotes to
T4116 479-480 DT denotes a
T4117 481-486 JJ denotes clean
T4118 487-497 JJ denotes prechilled
T4119 498-502 NN denotes tube
T4120 503-506 CC denotes and
T4121 507-513 VB denotes stored
T4122 514-516 IN denotes at
T4123 517-522 NN denotes −80°C
T4124 523-528 IN denotes until
T4125 529-532 NN denotes use
T4126 534-538 NN denotes EMSA
T4127 539-542 VB denotes was
T4128 543-552 VB denotes performed
T4129 553-555 IN denotes as
T4130 556-565 VB denotes described
T4131 566-576 RB denotes previously
T4132 577-578 -LRB- denotes [
T4133 578-579 CD denotes 8
T4134 579-580 -RRB- denotes ]
T4135 582-586 NN denotes NFκB
T4136 587-594 NN denotes binding
T4137 595-603 NN denotes activity
T4138 604-607 VB denotes was
T4139 608-616 VB denotes examined
T4140 617-619 IN denotes in
T4141 620-621 DT denotes a
T4142 622-627 NN denotes 15 μL
T4143 628-635 NN denotes binding
T4144 636-644 NN denotes reaction
T4145 645-652 NN denotes mixture
T4146 653-663 VB denotes containing
T4147 664-669 NN denotes 15 μg
T4148 670-672 IN denotes of
T4149 673-680 JJ denotes nuclear
T4150 681-689 NN denotes proteins
T4151 690-693 CC denotes and
T4152 694-701 NN denotes 35 fmol
T4153 702-703 -LRB- denotes [
T4154 703-708 NN denotes γ-32P
T4155 708-709 -RRB- denotes ]
T4156 710-717 VB denotes labeled
T4157 718-733 JJ denotes double-stranded
T4158 734-738 NN denotes NFκB
T4159 739-748 NN denotes consensus
T4160 749-764 NN denotes oligonucleotide
T4161 766-767 DT denotes A
T4162 768-773 JJ denotes super
T4163 774-779 NN denotes shift
T4164 780-785 NN denotes assay
T4165 786-791 VB denotes using
T4166 792-802 NN denotes antibodies
T4167 803-805 TO denotes to
T4168 806-809 NN denotes P65
T4169 810-813 CC denotes and
T4170 814-817 NN denotes P50
T4171 818-821 VB denotes was
T4172 822-831 VB denotes performed
T4173 832-834 TO denotes to
T4174 835-842 VB denotes confirm
T4175 843-847 NN denotes NFκB
T4176 848-855 NN denotes binding
T4177 856-867 NN denotes specificity
T4178 868-870 IN denotes as
T4179 871-881 RB denotes previously
T4180 882-891 VB denotes described
R3109 T4038 T4035 arg1Of Assay,Electrophoretic
R3110 T4038 T4036 arg1Of Assay,Mobility
R3111 T4038 T4037 arg1Of Assay,Shift
R3112 T4038 T4039 arg1Of Assay,(
R3113 T4040 T4039 arg2Of EMSA,(
R3114 T4041 T4039 arg3Of ),(
R3115 T4043 T4042 arg1Of proteins,Nuclear
R3116 T4043 T4044 arg1Of proteins,were
R3117 T4043 T4045 arg2Of proteins,isolated
R3118 T4045 T4044 arg2Of isolated,were
R3119 T4045 T4046 arg1Of isolated,from
R3120 T4048 T4046 arg2Of tissue,from
R3121 T4048 T4047 arg1Of tissue,brain
R3122 T4052 T4051 arg2Of removal,after
R3123 T4052 T4053 arg1Of removal,of
R3124 T4054 T4053 arg2Of supernatant,of
R3125 T4054 T4055 arg1Of supernatant,(
R3126 T4057 T4055 arg2Of extract,(
R3127 T4057 T4056 arg1Of extract,cytoplasmic
R3128 T4058 T4055 arg3Of ),(
R3129 T4061 T4060 arg1Of pellets,the
R3130 T4061 T4062 arg1Of pellets,were
R3131 T4061 T4063 arg2Of pellets,resuspended
R3132 T4063 T4049 arg1Of resuspended,Briefly
R3133 T4063 T4050 arg1Of resuspended,","
R3134 T4063 T4051 arg1Of resuspended,after
R3135 T4063 T4059 arg1Of resuspended,","
R3136 T4063 T4062 arg2Of resuspended,were
R3137 T4063 T4064 arg1Of resuspended,in
R3138 T4067 T4064 arg2Of reagent,in
R3139 T4067 T4065 arg1Of reagent,nuclear
R3140 T4067 T4066 arg1Of reagent,extraction
R3141 T4069 T4068 arg1Of tubes,The
R3142 T4069 T4070 arg1Of tubes,containing
R3143 T4069 T4072 arg1Of tubes,were
R3144 T4069 T4073 arg2Of tubes,put
R3145 T4071 T4070 arg2Of pellets,containing
R3146 T4073 T4072 arg2Of put,were
R3147 T4073 T4074 arg1Of put,on
R3148 T4073 T4076 arg1Of put,for
R3149 T4073 T4081 arg1Of put,minutes
R3150 T4073 T4082 arg1Of put,","
R3151 T4073 T4083 arg1Of put,for
R3152 T4075 T4074 arg2Of vortex,on
R3153 T4078 T4076 arg2Of seconds,for
R3154 T4078 T4077 arg1Of seconds,15
R3155 T4081 T4079 arg1Of minutes,every
R3156 T4081 T4080 arg1Of minutes,10
R3157 T4085 T4083 arg2Of total,for
R3158 T4085 T4084 arg1Of total,a
R3159 T4085 T4086 arg1Of total,of
R3160 T4088 T4086 arg2Of minutes,of
R3161 T4088 T4087 arg1Of minutes,40
R3162 T4090 T4089 arg1Of tubes,The
R3163 T4090 T4091 arg1Of tubes,were
R3164 T4090 T4092 arg2Of tubes,centrifuged
R3165 T4092 T4091 arg2Of centrifuged,were
R3166 T4092 T4093 arg1Of centrifuged,at
R3167 T4095 T4093 arg2Of speed,at
R3168 T4095 T4094 arg1Of speed,maximum
R3169 T4095 T4096 arg1Of speed,(
R3170 T4095 T4100 arg1Of speed,in
R3171 T4098 T4096 arg2Of × g,(
R3172 T4098 T4097 arg1Of × g,"~16,000"
R3173 T4099 T4096 arg3Of ),(
R3174 T4103 T4100 arg2Of centrifuge,in
R3175 T4103 T4101 arg1Of centrifuge,a
R3176 T4103 T4102 arg1Of centrifuge,micro
R3177 T4103 T4104 arg1Of centrifuge,for
R3178 T4106 T4104 arg2Of minutes,for
R3179 T4106 T4105 arg1Of minutes,10
R3180 T4108 T4107 arg1Of supernatants,The
R3181 T4108 T4109 arg1Of supernatants,(
R3182 T4108 T4113 arg1Of supernatants,were
R3183 T4108 T4114 arg2Of supernatants,transferred
R3184 T4108 T4121 arg2Of supernatants,stored
R3185 T4111 T4109 arg2Of extract,(
R3186 T4111 T4110 arg1Of extract,nuclear
R3187 T4112 T4109 arg3Of ),(
R3188 T4114 T4115 arg1Of transferred,to
R3189 T4114 T4120 arg1Of transferred,and
R3190 T4119 T4115 arg2Of tube,to
R3191 T4119 T4116 arg1Of tube,a
R3192 T4119 T4117 arg1Of tube,clean
R3193 T4119 T4118 arg1Of tube,prechilled
R3194 T4120 T4113 arg2Of and,were
R3195 T4121 T4120 arg2Of stored,and
R3196 T4121 T4122 arg1Of stored,at
R3197 T4121 T4124 arg1Of stored,until
R3198 T4123 T4122 arg2Of −80°C,at
R3199 T4125 T4124 arg2Of use,until
R3200 T4126 T4127 arg1Of EMSA,was
R3201 T4126 T4128 arg2Of EMSA,performed
R3202 T4128 T4127 arg2Of performed,was
R3203 T4128 T4129 arg1Of performed,as
R3204 T4128 T4132 arg1Of performed,[
R3205 T4130 T4129 arg2Of described,as
R3206 T4130 T4131 arg1Of described,previously
R3207 T4133 T4132 arg2Of 8,[
R3208 T4134 T4132 arg3Of ],[
R3209 T4137 T4135 arg1Of activity,NFκB
R3210 T4137 T4136 arg1Of activity,binding
R3211 T4137 T4138 arg1Of activity,was
R3212 T4137 T4139 arg2Of activity,examined
R3213 T4139 T4138 arg2Of examined,was
R3214 T4139 T4140 arg1Of examined,in
R3215 T4145 T4140 arg2Of mixture,in
R3216 T4145 T4141 arg1Of mixture,a
R3217 T4145 T4142 arg1Of mixture,15 μL
R3218 T4145 T4143 arg1Of mixture,binding
R3219 T4145 T4144 arg1Of mixture,reaction
R3220 T4145 T4146 arg1Of mixture,containing
R3221 T4147 T4148 arg1Of 15 μg,of
R3222 T4147 T4151 arg1Of 15 μg,and
R3223 T4150 T4148 arg2Of proteins,of
R3224 T4150 T4149 arg1Of proteins,nuclear
R3225 T4151 T4146 arg2Of and,containing
R3226 T4152 T4153 arg1Of 35 fmol,[
R3227 T4154 T4153 arg2Of γ-32P,[
R3228 T4155 T4153 arg3Of ],[
R3229 T4160 T4151 arg2Of oligonucleotide,and
R3230 T4160 T4152 arg1Of oligonucleotide,35 fmol
R3231 T4160 T4156 arg2Of oligonucleotide,labeled
R3232 T4160 T4157 arg1Of oligonucleotide,double-stranded
R3233 T4160 T4158 arg1Of oligonucleotide,NFκB
R3234 T4160 T4159 arg1Of oligonucleotide,consensus
R3235 T4164 T4161 arg1Of assay,A
R3236 T4164 T4162 arg1Of assay,super
R3237 T4164 T4163 arg1Of assay,shift
R3238 T4164 T4165 arg1Of assay,using
R3239 T4164 T4171 arg1Of assay,was
R3240 T4164 T4172 arg2Of assay,performed
R3241 T4166 T4165 arg2Of antibodies,using
R3242 T4166 T4167 arg1Of antibodies,to
R3243 T4168 T4169 arg1Of P65,and
R3244 T4169 T4167 arg2Of and,to
R3245 T4170 T4169 arg2Of P50,and
R3246 T4172 T4171 arg2Of performed,was
R3247 T4172 T4173 modOf performed,to
R3248 T4174 T4173 arg1Of confirm,to
R3249 T4177 T4174 arg2Of specificity,confirm
R3250 T4177 T4175 arg1Of specificity,NFκB
R3251 T4177 T4176 arg1Of specificity,binding
R3252 T4177 T4178 arg1Of specificity,as
R3253 T4180 T4178 arg2Of described,as
R3254 T4180 T4179 arg1Of described,previously