PMC:539061 / 33902-45472 JSON TXT 5 Projects

Annnotations TAB TSV DIC JSON TextAE

Id	Subject	Object	Predicate	Lexical cue
T7354	0-7	VBG	denotes	Probing
T7356	8-11	DT	denotes	the
T7357	12-22	NN	denotes	Regulation
T7358	23-25	IN	denotes	of
T7359	26-31	NN	denotes	Snail
T7360	32-36	NN	denotes	Gene
T7361	37-47	NN	denotes	Expression
T7355	48-55	VBZ	denotes	Reveals
T7362	56-58	DT	denotes	an
T7364	59-68	JJ	denotes	Essential
T7363	69-73	NN	denotes	Link
T7365	74-76	IN	denotes	to
T7366	77-80	NN	denotes	TGF
T7368	80-81	HYPH	denotes	-
T7367	81-83	NN	denotes	β2
T7369	84-93	NN	denotes	Signaling
T7370	94-96	IN	denotes	in
T7371	97-100	DT	denotes	the
T7373	101-111	VBG	denotes	Developing
T7374	112-116	NN	denotes	Hair
T7372	117-120	NN	denotes	Bud
T7375	120-253	sentence	denotes	The temporal spike of Snail mRNA expression in the hair bud prompted us to consider what factor(s) may be regulating the Snail gene.
T7376	121-124	DT	denotes	The
T7378	125-133	JJ	denotes	temporal
T7377	134-139	NN	denotes	spike
T7380	140-142	IN	denotes	of
T7381	143-148	NN	denotes	Snail
T7383	149-153	NN	denotes	mRNA
T7382	154-164	NN	denotes	expression
T7384	165-167	IN	denotes	in
T7385	168-171	DT	denotes	the
T7387	172-176	NN	denotes	hair
T7386	177-180	NN	denotes	bud
T7379	181-189	VBD	denotes	prompted
T7388	190-192	PRP	denotes	us
T7389	193-195	TO	denotes	to
T7390	196-204	VB	denotes	consider
T7391	205-209	WDT	denotes	what
T7392	210-216	NN	denotes	factor
T7394	216-217	-LRB-	denotes	(
T7395	217-218	AFX	denotes	s
T7396	218-219	-RRB-	denotes	)
T7397	220-223	MD	denotes	may
T7398	224-226	VB	denotes	be
T7393	227-237	VBG	denotes	regulating
T7399	238-241	DT	denotes	the
T7401	242-247	NN	denotes	Snail
T7400	248-252	NN	denotes	gene
T7402	252-253	.	denotes	.
T7403	253-477	sentence	denotes	A variety of extracellular signals have an impact on the cell type-specific expression of different Snail family members, and many of them, including Wnts, BMPs, FGFs, and TGF-βs, also affect hair bud development [2,16,28].
T7404	254-255	DT	denotes	A
T7405	256-263	NN	denotes	variety
T7407	264-266	IN	denotes	of
T7408	267-280	JJ	denotes	extracellular
T7409	281-288	NNS	denotes	signals
T7406	289-293	VBP	denotes	have
T7410	294-296	DT	denotes	an
T7411	297-303	NN	denotes	impact
T7412	304-306	IN	denotes	on
T7413	307-310	DT	denotes	the
T7415	311-315	NN	denotes	cell
T7416	316-320	NN	denotes	type
T7418	320-321	HYPH	denotes	-
T7417	321-329	JJ	denotes	specific
T7414	330-340	NN	denotes	expression
T7419	341-343	IN	denotes	of
T7420	344-353	JJ	denotes	different
T7422	354-359	NN	denotes	Snail
T7423	360-366	NN	denotes	family
T7421	367-374	NNS	denotes	members
T7424	374-376	,	denotes	,
T7425	376-379	CC	denotes	and
T7426	380-384	JJ	denotes	many
T7428	385-387	IN	denotes	of
T7429	388-392	PRP	denotes	them
T7430	392-394	,	denotes	,
T7431	394-403	VBG	denotes	including
T7432	404-408	NNS	denotes	Wnts
T7433	408-410	,	denotes	,
T7434	410-414	NNS	denotes	BMPs
T7435	414-416	,	denotes	,
T7436	416-420	NNS	denotes	FGFs
T7437	420-422	,	denotes	,
T7438	422-425	CC	denotes	and
T7439	426-429	NN	denotes	TGF
T7441	429-430	HYPH	denotes	-
T7440	430-432	NNS	denotes	βs
T7442	432-434	,	denotes	,
T7443	434-438	RB	denotes	also
T7427	439-445	VBP	denotes	affect
T7444	446-450	NN	denotes	hair
T7445	451-454	NN	denotes	bud
T7446	455-466	NN	denotes	development
T7447	467-468	-LRB-	denotes	[
T7449	468-469	CD	denotes	2
T7450	469-470	,	denotes	,
T7451	470-472	CD	denotes	16
T7452	472-473	,	denotes	,
T7448	473-475	CD	denotes	28
T7453	475-476	-RRB-	denotes	]
T7454	476-477	.	denotes	.
T7455	477-705	sentence	denotes	Since Snail is not expressed in cultured skin keratinocytes that secrete active BMPs and FGFs (see Figure 1B), we focused our attention on Wnt and TGF-β signaling as more likely candidates for Snail induction in this cell type.
T7456	478-483	IN	denotes	Since
T7458	484-489	NN	denotes	Snail
T7459	490-492	VBZ	denotes	is
T7460	493-496	RB	denotes	not
T7457	497-506	VBN	denotes	expressed
T7462	507-509	IN	denotes	in
T7463	510-518	VBN	denotes	cultured
T7465	519-523	NN	denotes	skin
T7464	524-537	NNS	denotes	keratinocytes
T7466	538-542	WDT	denotes	that
T7467	543-550	VBP	denotes	secrete
T7468	551-557	JJ	denotes	active
T7469	558-562	NNS	denotes	BMPs
T7470	563-566	CC	denotes	and
T7471	567-571	NNS	denotes	FGFs
T7472	572-573	-LRB-	denotes	(
T7473	573-576	VB	denotes	see
T7474	577-583	NN	denotes	Figure
T7475	584-586	NN	denotes	1B
T7476	586-587	-RRB-	denotes	)
T7477	587-589	,	denotes	,
T7478	589-591	PRP	denotes	we
T7461	592-599	VBD	denotes	focused
T7479	600-603	PRP$	denotes	our
T7480	604-613	NN	denotes	attention
T7481	614-616	IN	denotes	on
T7482	617-620	NN	denotes	Wnt
T7484	621-624	CC	denotes	and
T7485	625-628	NN	denotes	TGF
T7487	628-629	HYPH	denotes	-
T7486	629-630	NN	denotes	β
T7483	631-640	NN	denotes	signaling
T7488	641-643	IN	denotes	as
T7489	644-648	RBR	denotes	more
T7490	649-655	JJ	denotes	likely
T7491	656-666	NNS	denotes	candidates
T7492	667-670	IN	denotes	for
T7493	671-676	NN	denotes	Snail
T7494	677-686	NN	denotes	induction
T7495	687-689	IN	denotes	in
T7496	690-694	DT	denotes	this
T7498	695-699	NN	denotes	cell
T7497	700-704	NN	denotes	type
T7499	704-705	.	denotes	.
T7500	705-904	sentence	denotes	Previously, we showed that effective transmission of a Wnt-3a signal in cultured keratinocytes can be achieved through their exposure to the BMP inhibitor noggin, which induces LEF-1 expression [4].
T7501	706-716	RB	denotes	Previously
T7503	716-718	,	denotes	,
T7504	718-720	PRP	denotes	we
T7502	721-727	VBD	denotes	showed
T7505	728-732	IN	denotes	that
T7507	733-742	JJ	denotes	effective
T7508	743-755	NN	denotes	transmission
T7509	756-758	IN	denotes	of
T7510	759-760	DT	denotes	a
T7512	761-764	NN	denotes	Wnt
T7513	764-765	HYPH	denotes	-
T7514	765-767	CD	denotes	3a
T7511	768-774	NN	denotes	signal
T7515	775-777	IN	denotes	in
T7516	778-786	VBN	denotes	cultured
T7517	787-800	NNS	denotes	keratinocytes
T7518	801-804	MD	denotes	can
T7519	805-807	VB	denotes	be
T7506	808-816	VBN	denotes	achieved
T7520	817-824	IN	denotes	through
T7521	825-830	PRP$	denotes	their
T7522	831-839	NN	denotes	exposure
T7523	840-842	IN	denotes	to
T7524	843-846	DT	denotes	the
T7526	847-850	NN	denotes	BMP
T7525	851-860	NN	denotes	inhibitor
T7527	861-867	NN	denotes	noggin
T7528	867-869	,	denotes	,
T7529	869-874	WDT	denotes	which
T7530	875-882	VBZ	denotes	induces
T7531	883-886	NN	denotes	LEF
T7533	886-887	HYPH	denotes	-
T7534	887-888	CD	denotes	1
T7532	889-899	NN	denotes	expression
T7535	900-901	-LRB-	denotes	[
T7536	901-902	CD	denotes	4
T7537	902-903	-RRB-	denotes	]
T7538	903-904	.	denotes	.
T7539	904-1039	sentence	denotes	In vitro, these conditions down-regulated the E-cadherin promoter and induced a LEF-1/β-catenin-sensitive reporter gene, TOPFLASH [4].
T7540	905-907	FW	denotes	In
T7541	908-913	FW	denotes	vitro
T7543	913-915	,	denotes	,
T7544	915-920	DT	denotes	these
T7545	921-931	NNS	denotes	conditions
T7546	932-936	RB	denotes	down
T7547	936-937	HYPH	denotes	-
T7542	937-946	VBD	denotes	regulated
T7548	947-950	DT	denotes	the
T7550	951-952	NN	denotes	E
T7552	952-953	HYPH	denotes	-
T7551	953-961	NN	denotes	cadherin
T7549	962-970	NN	denotes	promoter
T7553	971-974	CC	denotes	and
T7554	975-982	VBD	denotes	induced
T7555	983-984	DT	denotes	a
T7557	985-988	NN	denotes	LEF
T7559	988-989	HYPH	denotes	-
T7560	989-990	CD	denotes	1
T7561	990-991	HYPH	denotes	/
T7562	991-992	NN	denotes	β
T7564	992-993	HYPH	denotes	-
T7563	993-1000	NN	denotes	catenin
T7565	1000-1001	HYPH	denotes	-
T7558	1001-1010	JJ	denotes	sensitive
T7566	1011-1019	NN	denotes	reporter
T7556	1020-1024	NN	denotes	gene
T7567	1024-1026	,	denotes	,
T7568	1026-1034	NN	denotes	TOPFLASH
T7569	1035-1036	-LRB-	denotes	[
T7570	1036-1037	CD	denotes	4
T7571	1037-1038	-RRB-	denotes	]
T7572	1038-1039	.	denotes	.
T7573	1039-1118	sentence	denotes	In contrast, Snail expression was not induced by these conditions (Figure 5A).
T7574	1040-1042	IN	denotes	In
T7576	1043-1051	NN	denotes	contrast
T7577	1051-1053	,	denotes	,
T7578	1053-1058	NN	denotes	Snail
T7579	1059-1069	NN	denotes	expression
T7580	1070-1073	VBD	denotes	was
T7581	1074-1077	RB	denotes	not
T7575	1078-1085	VBN	denotes	induced
T7582	1086-1088	IN	denotes	by
T7583	1089-1094	DT	denotes	these
T7584	1095-1105	NNS	denotes	conditions
T7585	1106-1107	-LRB-	denotes	(
T7587	1107-1113	NN	denotes	Figure
T7586	1114-1116	NN	denotes	5A
T7588	1116-1117	-RRB-	denotes	)
T7589	1117-1118	.	denotes	.
T7590	1118-1322	sentence	denotes	Thus, despite essential roles for Wnts and noggin in hair follicle specification [4,29,30], our studies did not support an essential role for these signals in governing Snail expression in keratinocytes.
T7591	1119-1123	RB	denotes	Thus
T7593	1123-1125	,	denotes	,
T7594	1125-1132	IN	denotes	despite
T7595	1133-1142	JJ	denotes	essential
T7596	1143-1148	NNS	denotes	roles
T7597	1149-1152	IN	denotes	for
T7598	1153-1157	NNS	denotes	Wnts
T7599	1158-1161	CC	denotes	and
T7600	1162-1168	NN	denotes	noggin
T7601	1169-1171	IN	denotes	in
T7602	1172-1176	NN	denotes	hair
T7603	1177-1185	NN	denotes	follicle
T7604	1186-1199	NN	denotes	specification
T7605	1200-1201	-LRB-	denotes	[
T7607	1201-1202	CD	denotes	4
T7608	1202-1203	,	denotes	,
T7609	1203-1205	CD	denotes	29
T7610	1205-1206	,	denotes	,
T7606	1206-1208	CD	denotes	30
T7611	1208-1209	-RRB-	denotes	]
T7612	1209-1211	,	denotes	,
T7613	1211-1214	PRP$	denotes	our
T7614	1215-1222	NNS	denotes	studies
T7615	1223-1226	VBD	denotes	did
T7616	1227-1230	RB	denotes	not
T7592	1231-1238	VB	denotes	support
T7617	1239-1241	DT	denotes	an
T7619	1242-1251	JJ	denotes	essential
T7618	1252-1256	NN	denotes	role
T7620	1257-1260	IN	denotes	for
T7621	1261-1266	DT	denotes	these
T7622	1267-1274	NNS	denotes	signals
T7623	1275-1277	IN	denotes	in
T7624	1278-1287	VBG	denotes	governing
T7625	1288-1293	NN	denotes	Snail
T7626	1294-1304	NN	denotes	expression
T7627	1305-1307	IN	denotes	in
T7628	1308-1321	NNS	denotes	keratinocytes
T7629	1321-1322	.	denotes	.
T7630	1322-3521	sentence	denotes	Figure 5 TGF-β2, but Not Wnt/noggin, Transiently Induces Snail Expression In Vitro (A) Failure of Wnt and noggin signaling to induce Snail in cultured keratinocytes. Primary mouse keratinocytes were treated with Wnt- and/or noggin-conditioned medium (+) or the corresponding control medium (–). These conditions are known to activate the LEF-1/β-catenin reporter TOPGal and down-regulate the E-cadherin promoter (see [4] for details). Using Western blot analyses, cellular proteins were then analyzed for Snail, LEF-1, β-catenin, and tubulin. Proteins from keratinocytes transfected with K14-Snail were used as a positive control for Snail expression. (B) TGF-β2 can induce Snail protein. Primary keratinocytes were treated for the indicated times with recombinant TGF-β2 (+) or heat inactivated TGF-β2 (–).Total cellular proteins were then isolated and analyzed by Western blot for Snail, pSMAD2 (reflective of activated TGF- signaling), and tubulin. Note the activation of Snail expression, peaking at 2 h post-TGF-β2 treatment and then disappearing thereafter. (C) Snail mRNA expression is transiently induced by TGF-β2. The experiment in (B) was repeated, and this time, total RNAs were isolated from keratinocytes treated with TGF-β2 for the indicated times. RT-PCR was then used with (+) or without (–) reverse transcriptase (RT) and with primer sets specific for Snail and GAPDH mRNAs. Note that Snail mRNA expression also peaked at 2 h, paralleling Snail protein. (D) TGF-β2 treatment results in enhanced activity of a Snail promoter-β-galactosidase reporter. Keratinocytes were transfected with a β-galactosidase reporter driven by a Snail promoter that is either WT (wt prom) or harbors a mutation in a putative pSMAD2/pSMAD4 binding site (mt prom). At 2 d posttransfection, cells were treated with either TGF-β or heat-inactivated TGF-β2 (inact) for the times indicated. β-galactosidase assays were then conducted, and results are reported as fold increase over a basal level of activity of 1. The experiment was repeated three times in triplicate, and error bars reflect variations in the results. TGF-β1 has been shown to induce Snail family members in hepatocytes and heart [15, 31].
T7631	3434-3437	NN	denotes	TGF
T7633	3437-3438	HYPH	denotes	-
T7632	3438-3440	NN	denotes	β1
T7635	3441-3444	VBZ	denotes	has
T7636	3445-3449	VBN	denotes	been
T7634	3450-3455	VBN	denotes	shown
T7637	3456-3458	TO	denotes	to
T7638	3459-3465	VB	denotes	induce
T7639	3466-3471	NN	denotes	Snail
T7641	3472-3478	NN	denotes	family
T7640	3479-3486	NNS	denotes	members
T7642	3487-3489	IN	denotes	in
T7643	3490-3501	NNS	denotes	hepatocytes
T7644	3502-3505	CC	denotes	and
T7645	3506-3511	NN	denotes	heart
T7646	3512-3513	-LRB-	denotes	[
T7648	3513-3515	CD	denotes	15
T7649	3515-3517	,	denotes	,
T7647	3517-3519	CD	denotes	31
T7650	3519-3520	-RRB-	denotes	]
T7651	3520-3521	.	denotes	.
T7652	3521-3680	sentence	denotes	In keratinocytes, however, TGF-β1 inhibits keratinocyte growth and seems to be involved in triggering the destructive phase of the cycling hair follicle [32].
T7653	3522-3524	IN	denotes	In
T7655	3525-3538	NNS	denotes	keratinocytes
T7656	3538-3540	,	denotes	,
T7657	3540-3547	RB	denotes	however
T7658	3547-3549	,	denotes	,
T7659	3549-3552	NN	denotes	TGF
T7661	3552-3553	HYPH	denotes	-
T7660	3553-3555	NN	denotes	β1
T7654	3556-3564	VBZ	denotes	inhibits
T7662	3565-3577	NN	denotes	keratinocyte
T7663	3578-3584	NN	denotes	growth
T7664	3585-3588	CC	denotes	and
T7665	3589-3594	VBZ	denotes	seems
T7666	3595-3597	TO	denotes	to
T7668	3598-3600	VB	denotes	be
T7667	3601-3609	VBN	denotes	involved
T7669	3610-3612	IN	denotes	in
T7670	3613-3623	VBG	denotes	triggering
T7671	3624-3627	DT	denotes	the
T7673	3628-3639	JJ	denotes	destructive
T7672	3640-3645	NN	denotes	phase
T7674	3646-3648	IN	denotes	of
T7675	3649-3652	DT	denotes	the
T7677	3653-3660	VBG	denotes	cycling
T7678	3661-3665	NN	denotes	hair
T7676	3666-3674	NN	denotes	follicle
T7679	3675-3676	-LRB-	denotes	[
T7680	3676-3678	CD	denotes	32
T7681	3678-3679	-RRB-	denotes	]
T7682	3679-3680	.	denotes	.
T7683	3680-3836	sentence	denotes	Of the loss-of-function mutations generated in each of the TGF-β genes, only the TGF-β2 null state blocked follicle development at the hair bud stage [32].
T7684	3681-3683	IN	denotes	Of
T7686	3684-3687	DT	denotes	the
T7688	3688-3692	NN	denotes	loss
T7689	3692-3693	HYPH	denotes	-
T7690	3693-3695	IN	denotes	of
T7691	3695-3696	HYPH	denotes	-
T7692	3696-3704	NN	denotes	function
T7687	3705-3714	NNS	denotes	mutations
T7693	3715-3724	VBN	denotes	generated
T7694	3725-3727	IN	denotes	in
T7695	3728-3732	DT	denotes	each
T7696	3733-3735	IN	denotes	of
T7697	3736-3739	DT	denotes	the
T7699	3740-3743	NN	denotes	TGF
T7701	3743-3744	HYPH	denotes	-
T7700	3744-3745	NN	denotes	β
T7698	3746-3751	NNS	denotes	genes
T7702	3751-3753	,	denotes	,
T7703	3753-3757	RB	denotes	only
T7705	3758-3761	DT	denotes	the
T7706	3762-3765	NN	denotes	TGF
T7708	3765-3766	HYPH	denotes	-
T7707	3766-3768	NN	denotes	β2
T7709	3769-3773	JJ	denotes	null
T7704	3774-3779	NN	denotes	state
T7685	3780-3787	VBD	denotes	blocked
T7710	3788-3796	NN	denotes	follicle
T7711	3797-3808	NN	denotes	development
T7712	3809-3811	IN	denotes	at
T7713	3812-3815	DT	denotes	the
T7715	3816-3820	NN	denotes	hair
T7716	3821-3824	NN	denotes	bud
T7714	3825-3830	NN	denotes	stage
T7717	3831-3832	-LRB-	denotes	[
T7718	3832-3834	CD	denotes	32
T7719	3834-3835	-RRB-	denotes	]
T7720	3835-3836	.	denotes	.
T7721	3836-4000	sentence	denotes	Thus, we turned towards addressing whether TGF-β2 might be involved in regulating Snail expression in keratinocytes isolated from the basal layer of the epidermis.
T7722	3837-3841	RB	denotes	Thus
T7724	3841-3843	,	denotes	,
T7725	3843-3845	PRP	denotes	we
T7723	3846-3852	VBD	denotes	turned
T7726	3853-3860	IN	denotes	towards
T7727	3861-3871	VBG	denotes	addressing
T7728	3872-3879	IN	denotes	whether
T7730	3880-3883	NN	denotes	TGF
T7732	3883-3884	HYPH	denotes	-
T7731	3884-3886	NN	denotes	β2
T7733	3887-3892	MD	denotes	might
T7734	3893-3895	VB	denotes	be
T7729	3896-3904	VBN	denotes	involved
T7735	3905-3907	IN	denotes	in
T7736	3908-3918	VBG	denotes	regulating
T7737	3919-3924	NN	denotes	Snail
T7738	3925-3935	NN	denotes	expression
T7739	3936-3938	IN	denotes	in
T7740	3939-3952	NNS	denotes	keratinocytes
T7741	3953-3961	VBN	denotes	isolated
T7742	3962-3966	IN	denotes	from
T7743	3967-3970	DT	denotes	the
T7745	3971-3976	JJ	denotes	basal
T7744	3977-3982	NN	denotes	layer
T7746	3983-3985	IN	denotes	of
T7747	3986-3989	DT	denotes	the
T7748	3990-3999	NN	denotes	epidermis
T7749	3999-4000	.	denotes	.
T7750	4000-4231	sentence	denotes	Though there is no cell culture system available to specifically study placodal cells, these keratinocytes are their progenitors and are the closest approximation available to study the behavior of epithelial cells of the placode.
T7751	4001-4007	IN	denotes	Though
T7753	4008-4013	EX	denotes	there
T7752	4014-4016	VBZ	denotes	is
T7755	4017-4019	DT	denotes	no
T7757	4020-4024	NN	denotes	cell
T7758	4025-4032	NN	denotes	culture
T7756	4033-4039	NN	denotes	system
T7759	4040-4049	JJ	denotes	available
T7760	4050-4052	TO	denotes	to
T7762	4053-4065	RB	denotes	specifically
T7761	4066-4071	VB	denotes	study
T7763	4072-4080	JJ	denotes	placodal
T7764	4081-4086	NNS	denotes	cells
T7765	4086-4088	,	denotes	,
T7766	4088-4093	DT	denotes	these
T7767	4094-4107	NNS	denotes	keratinocytes
T7754	4108-4111	VBP	denotes	are
T7768	4112-4117	PRP$	denotes	their
T7769	4118-4129	NNS	denotes	progenitors
T7770	4130-4133	CC	denotes	and
T7771	4134-4137	VBP	denotes	are
T7772	4138-4141	DT	denotes	the
T7774	4142-4149	JJS	denotes	closest
T7773	4150-4163	NN	denotes	approximation
T7775	4164-4173	JJ	denotes	available
T7776	4174-4176	TO	denotes	to
T7777	4177-4182	VB	denotes	study
T7778	4183-4186	DT	denotes	the
T7779	4187-4195	NN	denotes	behavior
T7780	4196-4198	IN	denotes	of
T7781	4199-4209	JJ	denotes	epithelial
T7782	4210-4215	NNS	denotes	cells
T7783	4216-4218	IN	denotes	of
T7784	4219-4222	DT	denotes	the
T7785	4223-4230	NN	denotes	placode
T7786	4230-4231	.	denotes	.
T7787	4231-4379	sentence	denotes	Interestingly, treatment of cultured keratinocytes with as little as 5 ng/ml of TGF-β2 caused a rapid and transient induction of Snail (Figure 5B).
T7788	4232-4245	RB	denotes	Interestingly
T7790	4245-4247	,	denotes	,
T7791	4247-4256	NN	denotes	treatment
T7792	4257-4259	IN	denotes	of
T7793	4260-4268	VBN	denotes	cultured
T7794	4269-4282	NNS	denotes	keratinocytes
T7795	4283-4287	IN	denotes	with
T7796	4288-4290	RB	denotes	as
T7798	4291-4297	JJ	denotes	little
T7799	4298-4300	IN	denotes	as
T7797	4301-4302	CD	denotes	5
T7800	4303-4305	NN	denotes	ng
T7801	4305-4306	SYM	denotes	/
T7802	4306-4308	NN	denotes	ml
T7803	4309-4311	IN	denotes	of
T7804	4312-4315	NN	denotes	TGF
T7806	4315-4316	HYPH	denotes	-
T7805	4316-4318	NN	denotes	β2
T7789	4319-4325	VBD	denotes	caused
T7807	4326-4327	DT	denotes	a
T7809	4328-4333	JJ	denotes	rapid
T7810	4334-4337	CC	denotes	and
T7811	4338-4347	JJ	denotes	transient
T7808	4348-4357	NN	denotes	induction
T7812	4358-4360	IN	denotes	of
T7813	4361-4366	NN	denotes	Snail
T7814	4367-4368	-LRB-	denotes	(
T7816	4368-4374	NN	denotes	Figure
T7815	4375-4377	NN	denotes	5B
T7817	4377-4378	-RRB-	denotes	)
T7818	4378-4379	.	denotes	.
T7819	4379-4492	sentence	denotes	Following this treatment, Snail protein was detected within 30 min, peaked at 2 h, and then declined thereafter.
T7820	4380-4389	VBG	denotes	Following
T7822	4390-4394	DT	denotes	this
T7823	4395-4404	NN	denotes	treatment
T7824	4404-4406	,	denotes	,
T7825	4406-4411	NN	denotes	Snail
T7826	4412-4419	NN	denotes	protein
T7827	4420-4423	VBD	denotes	was
T7821	4424-4432	VBN	denotes	detected
T7828	4433-4439	IN	denotes	within
T7829	4440-4442	CD	denotes	30
T7830	4443-4446	NN	denotes	min
T7831	4446-4448	,	denotes	,
T7832	4448-4454	VBD	denotes	peaked
T7833	4455-4457	IN	denotes	at
T7834	4458-4459	CD	denotes	2
T7835	4460-4461	NN	denotes	h
T7836	4461-4463	,	denotes	,
T7837	4463-4466	CC	denotes	and
T7838	4467-4471	RB	denotes	then
T7839	4472-4480	VBD	denotes	declined
T7840	4481-4491	RB	denotes	thereafter
T7841	4491-4492	.	denotes	.
T7842	4492-4684	sentence	denotes	The induction of Snail appeared to be specific for the active form of the growth factor, as pretreatment of TGF-β2 for 10 min at 100 °C obliterated the response [Figure 5B, lanes marked (–)].
T7843	4493-4496	DT	denotes	The
T7844	4497-4506	NN	denotes	induction
T7846	4507-4509	IN	denotes	of
T7847	4510-4515	NN	denotes	Snail
T7845	4516-4524	VBD	denotes	appeared
T7848	4525-4527	TO	denotes	to
T7849	4528-4530	VB	denotes	be
T7850	4531-4539	JJ	denotes	specific
T7851	4540-4543	IN	denotes	for
T7852	4544-4547	DT	denotes	the
T7854	4548-4554	JJ	denotes	active
T7853	4555-4559	NN	denotes	form
T7855	4560-4562	IN	denotes	of
T7856	4563-4566	DT	denotes	the
T7858	4567-4573	NN	denotes	growth
T7857	4574-4580	NN	denotes	factor
T7859	4580-4582	,	denotes	,
T7860	4582-4584	IN	denotes	as
T7862	4585-4597	NN	denotes	pretreatment
T7863	4598-4600	IN	denotes	of
T7864	4601-4604	NN	denotes	TGF
T7866	4604-4605	HYPH	denotes	-
T7865	4605-4607	NN	denotes	β2
T7867	4608-4611	IN	denotes	for
T7868	4612-4614	CD	denotes	10
T7869	4615-4618	NN	denotes	min
T7870	4619-4621	IN	denotes	at
T7871	4622-4625	CD	denotes	100
T7872	4626-4628	NN	denotes	°C
T7861	4629-4640	VBD	denotes	obliterated
T7873	4641-4644	DT	denotes	the
T7874	4645-4653	NN	denotes	response
T7875	4654-4655	-LRB-	denotes	[
T7877	4655-4661	NN	denotes	Figure
T7878	4662-4664	NN	denotes	5B
T7879	4664-4666	,	denotes	,
T7876	4666-4671	NNS	denotes	lanes
T7880	4672-4678	VBN	denotes	marked
T7881	4679-4680	-LRB-	denotes	(
T7882	4680-4681	SYM	denotes	–
T7883	4681-4682	-RRB-	denotes	)
T7884	4682-4683	-RRB-	denotes	]
T7885	4683-4684	.	denotes	.
T7886	4684-4922	sentence	denotes	By contrast, although TGF-β receptor activation remained elevated during the duration of the experiment (as measured by the sustained phosphorylation of the downstream effector SMAD2) Snail expression could not be maintained (Figure 5B).
T7887	4685-4687	IN	denotes	By
T7889	4688-4696	NN	denotes	contrast
T7890	4696-4698	,	denotes	,
T7891	4698-4706	IN	denotes	although
T7893	4707-4710	NN	denotes	TGF
T7895	4710-4711	HYPH	denotes	-
T7894	4711-4712	NN	denotes	β
T7896	4713-4721	NN	denotes	receptor
T7897	4722-4732	NN	denotes	activation
T7892	4733-4741	VBD	denotes	remained
T7898	4742-4750	JJ	denotes	elevated
T7899	4751-4757	IN	denotes	during
T7900	4758-4761	DT	denotes	the
T7901	4762-4770	NN	denotes	duration
T7902	4771-4773	IN	denotes	of
T7903	4774-4777	DT	denotes	the
T7904	4778-4788	NN	denotes	experiment
T7905	4789-4790	-LRB-	denotes	(
T7906	4790-4792	IN	denotes	as
T7907	4793-4801	VBN	denotes	measured
T7908	4802-4804	IN	denotes	by
T7909	4805-4808	DT	denotes	the
T7911	4809-4818	JJ	denotes	sustained
T7910	4819-4834	NN	denotes	phosphorylation
T7912	4835-4837	IN	denotes	of
T7913	4838-4841	DT	denotes	the
T7915	4842-4852	JJ	denotes	downstream
T7914	4853-4861	NN	denotes	effector
T7916	4862-4867	NN	denotes	SMAD2
T7917	4867-4868	-RRB-	denotes	)
T7918	4869-4874	NN	denotes	Snail
T7919	4875-4885	NN	denotes	expression
T7920	4886-4891	MD	denotes	could
T7921	4892-4895	RB	denotes	not
T7922	4896-4898	VB	denotes	be
T7888	4899-4909	VBN	denotes	maintained
T7923	4910-4911	-LRB-	denotes	(
T7925	4911-4917	NN	denotes	Figure
T7924	4918-4920	NN	denotes	5B
T7926	4920-4921	-RRB-	denotes	)
T7927	4921-4922	.	denotes	.
T7928	4922-5070	sentence	denotes	Thus, although Snail expression correlated with phosphorylated SMAD2 (pSMAD2) induction, its decline seemed to rely on secondary downstream events.
T7929	4923-4927	RB	denotes	Thus
T7931	4927-4929	,	denotes	,
T7932	4929-4937	IN	denotes	although
T7934	4938-4943	NN	denotes	Snail
T7935	4944-4954	NN	denotes	expression
T7933	4955-4965	VBD	denotes	correlated
T7936	4966-4970	IN	denotes	with
T7937	4971-4985	VBN	denotes	phosphorylated
T7938	4986-4991	NN	denotes	SMAD2
T7940	4992-4993	-LRB-	denotes	(
T7941	4993-4999	NN	denotes	pSMAD2
T7942	4999-5000	-RRB-	denotes	)
T7939	5001-5010	NN	denotes	induction
T7943	5010-5012	,	denotes	,
T7944	5012-5015	PRP$	denotes	its
T7945	5016-5023	NN	denotes	decline
T7930	5024-5030	VBD	denotes	seemed
T7946	5031-5033	TO	denotes	to
T7947	5034-5038	VB	denotes	rely
T7948	5039-5041	IN	denotes	on
T7949	5042-5051	JJ	denotes	secondary
T7951	5052-5062	JJ	denotes	downstream
T7950	5063-5069	NNS	denotes	events
T7952	5069-5070	.	denotes	.
T7953	5070-5252	sentence	denotes	The rapid kinetics of Snail expression were reflected at the mRNA level, suggesting that Snail promoter activity in keratinocytes might be sensitive to TGF-β2 signaling (Figure 5C).
T7954	5071-5074	DT	denotes	The
T7956	5075-5080	JJ	denotes	rapid
T7955	5081-5089	NNS	denotes	kinetics
T7958	5090-5092	IN	denotes	of
T7959	5093-5098	NN	denotes	Snail
T7960	5099-5109	NN	denotes	expression
T7961	5110-5114	VBD	denotes	were
T7957	5115-5124	VBN	denotes	reflected
T7962	5125-5127	IN	denotes	at
T7963	5128-5131	DT	denotes	the
T7965	5132-5136	NN	denotes	mRNA
T7964	5137-5142	NN	denotes	level
T7966	5142-5144	,	denotes	,
T7967	5144-5154	VBG	denotes	suggesting
T7968	5155-5159	IN	denotes	that
T7970	5160-5165	NN	denotes	Snail
T7971	5166-5174	NN	denotes	promoter
T7972	5175-5183	NN	denotes	activity
T7973	5184-5186	IN	denotes	in
T7974	5187-5200	NNS	denotes	keratinocytes
T7975	5201-5206	MD	denotes	might
T7969	5207-5209	VB	denotes	be
T7976	5210-5219	JJ	denotes	sensitive
T7977	5220-5222	IN	denotes	to
T7978	5223-5226	NN	denotes	TGF
T7980	5226-5227	HYPH	denotes	-
T7979	5227-5229	NN	denotes	β2
T7981	5230-5239	NN	denotes	signaling
T7982	5240-5241	-LRB-	denotes	(
T7984	5241-5247	NN	denotes	Figure
T7983	5248-5250	NN	denotes	5C
T7985	5250-5251	-RRB-	denotes	)
T7986	5251-5252	.	denotes	.
T7987	5252-5479	sentence	denotes	To test this possibility, we engineered a transgene driving the β-galactosidase reporter under the control of approximately 2.2 kb of promoter sequence located 5′ from the transcription initiation site of the mouse Snail gene.
T7988	5253-5255	TO	denotes	To
T7989	5256-5260	VB	denotes	test
T7991	5261-5265	DT	denotes	this
T7992	5266-5277	NN	denotes	possibility
T7993	5277-5279	,	denotes	,
T7994	5279-5281	PRP	denotes	we
T7990	5282-5292	VBD	denotes	engineered
T7995	5293-5294	DT	denotes	a
T7996	5295-5304	NN	denotes	transgene
T7997	5305-5312	VBG	denotes	driving
T7998	5313-5316	DT	denotes	the
T8000	5317-5318	NN	denotes	β
T8002	5318-5319	HYPH	denotes	-
T8001	5319-5332	NN	denotes	galactosidase
T7999	5333-5341	NN	denotes	reporter
T8003	5342-5347	IN	denotes	under
T8004	5348-5351	DT	denotes	the
T8005	5352-5359	NN	denotes	control
T8006	5360-5362	IN	denotes	of
T8007	5363-5376	RB	denotes	approximately
T8008	5377-5380	CD	denotes	2.2
T8009	5381-5383	NN	denotes	kb
T8010	5384-5386	IN	denotes	of
T8011	5387-5395	NN	denotes	promoter
T8012	5396-5404	NN	denotes	sequence
T8013	5405-5412	VBN	denotes	located
T8014	5413-5414	CD	denotes	5
T8015	5414-5415	SYM	denotes	′
T8016	5416-5420	IN	denotes	from
T8017	5421-5424	DT	denotes	the
T8019	5425-5438	NN	denotes	transcription
T8020	5439-5449	NN	denotes	initiation
T8018	5450-5454	NN	denotes	site
T8021	5455-5457	IN	denotes	of
T8022	5458-5461	DT	denotes	the
T8024	5462-5467	NN	denotes	mouse
T8025	5468-5473	NN	denotes	Snail
T8023	5474-5478	NN	denotes	gene
T8026	5478-5479	.	denotes	.
T8027	5479-5679	sentence	denotes	At 2 d after transient transfection, keratinocytes were treated with TGF-β2 (t = 0) and then assayed for transgene activity over the same time course in which we had observed Snail protein induction.
T8028	5480-5482	IN	denotes	At
T8030	5483-5484	CD	denotes	2
T8031	5485-5486	NN	denotes	d
T8032	5487-5492	IN	denotes	after
T8033	5493-5502	JJ	denotes	transient
T8034	5503-5515	NN	denotes	transfection
T8035	5515-5517	,	denotes	,
T8036	5517-5530	NNS	denotes	keratinocytes
T8037	5531-5535	VBD	denotes	were
T8029	5536-5543	VBN	denotes	treated
T8038	5544-5548	IN	denotes	with
T8039	5549-5552	NN	denotes	TGF
T8041	5552-5553	HYPH	denotes	-
T8040	5553-5555	NN	denotes	β2
T8042	5556-5557	-LRB-	denotes	(
T8044	5557-5558	NN	denotes	t
T8045	5559-5560	SYM	denotes	=
T8043	5561-5562	CD	denotes	0
T8046	5562-5563	-RRB-	denotes	)
T8047	5564-5567	CC	denotes	and
T8048	5568-5572	RB	denotes	then
T8049	5573-5580	VBN	denotes	assayed
T8050	5581-5584	IN	denotes	for
T8051	5585-5594	NN	denotes	transgene
T8052	5595-5603	NN	denotes	activity
T8053	5604-5608	IN	denotes	over
T8054	5609-5612	DT	denotes	the
T8056	5613-5617	JJ	denotes	same
T8057	5618-5622	NN	denotes	time
T8055	5623-5629	NN	denotes	course
T8058	5630-5632	IN	denotes	in
T8060	5633-5638	WDT	denotes	which
T8061	5639-5641	PRP	denotes	we
T8062	5642-5645	VBD	denotes	had
T8059	5646-5654	VBN	denotes	observed
T8063	5655-5660	NN	denotes	Snail
T8064	5661-5668	NN	denotes	protein
T8065	5669-5678	NN	denotes	induction
T8066	5678-5679	.	denotes	.
T8067	5679-5738	sentence	denotes	The results of this experiment are presented in Figure 5D.
T8068	5680-5683	DT	denotes	The
T8069	5684-5691	NNS	denotes	results
T8071	5692-5694	IN	denotes	of
T8072	5695-5699	DT	denotes	this
T8073	5700-5710	NN	denotes	experiment
T8074	5711-5714	VBP	denotes	are
T8070	5715-5724	VBN	denotes	presented
T8075	5725-5727	IN	denotes	in
T8076	5728-5734	NN	denotes	Figure
T8077	5735-5737	NN	denotes	5D
T8078	5737-5738	.	denotes	.
T8079	5738-5898	sentence	denotes	Within 0.5 h of TGF-β2 treatment, Snail promoter activity had increased 3-fold, and by 2 h, it peaked to approximately 10-fold over control levels (Figure 5D).
T8080	5739-5745	IN	denotes	Within
T8082	5746-5749	CD	denotes	0.5
T8083	5750-5751	NN	denotes	h
T8084	5752-5754	IN	denotes	of
T8085	5755-5758	NN	denotes	TGF
T8087	5758-5759	HYPH	denotes	-
T8086	5759-5761	NN	denotes	β2
T8088	5762-5771	NN	denotes	treatment
T8089	5771-5773	,	denotes	,
T8090	5773-5778	NN	denotes	Snail
T8091	5779-5787	NN	denotes	promoter
T8092	5788-5796	NN	denotes	activity
T8093	5797-5800	VBD	denotes	had
T8081	5801-5810	VBN	denotes	increased
T8094	5811-5817	RB	denotes	3-fold
T8095	5817-5819	,	denotes	,
T8096	5819-5822	CC	denotes	and
T8097	5823-5825	IN	denotes	by
T8099	5826-5827	CD	denotes	2
T8100	5828-5829	NN	denotes	h
T8101	5829-5831	,	denotes	,
T8102	5831-5833	PRP	denotes	it
T8098	5834-5840	VBD	denotes	peaked
T8103	5841-5843	IN	denotes	to
T8104	5844-5857	RB	denotes	approximately
T8105	5858-5865	RB	denotes	10-fold
T8106	5866-5870	IN	denotes	over
T8107	5871-5878	NN	denotes	control
T8108	5879-5885	NNS	denotes	levels
T8109	5886-5887	-LRB-	denotes	(
T8111	5887-5893	NN	denotes	Figure
T8110	5894-5896	NN	denotes	5D
T8112	5896-5897	-RRB-	denotes	)
T8113	5897-5898	.	denotes	.
T8114	5898-6007	sentence	denotes	Thereafter, Snail promoter activity rapidly returned to the basal levels seen in unstimulated keratinocytes.
T8115	5899-5909	RB	denotes	Thereafter
T8117	5909-5911	,	denotes	,
T8118	5911-5916	NN	denotes	Snail
T8119	5917-5925	NN	denotes	promoter
T8120	5926-5934	NN	denotes	activity
T8121	5935-5942	RB	denotes	rapidly
T8116	5943-5951	VBD	denotes	returned
T8122	5952-5954	IN	denotes	to
T8123	5955-5958	DT	denotes	the
T8125	5959-5964	JJ	denotes	basal
T8124	5965-5971	NNS	denotes	levels
T8126	5972-5976	VBN	denotes	seen
T8127	5977-5979	IN	denotes	in
T8128	5980-5992	JJ	denotes	unstimulated
T8129	5993-6006	NNS	denotes	keratinocytes
T8130	6006-6007	.	denotes	.
T8131	6007-6110	sentence	denotes	The kinetics of Snail promoter activity closely paralleled those observed for Snail protein induction.
T8132	6008-6011	DT	denotes	The
T8133	6012-6020	NNS	denotes	kinetics
T8135	6021-6023	IN	denotes	of
T8136	6024-6029	NN	denotes	Snail
T8137	6030-6038	NN	denotes	promoter
T8138	6039-6047	NN	denotes	activity
T8139	6048-6055	RB	denotes	closely
T8134	6056-6066	VBD	denotes	paralleled
T8140	6067-6072	DT	denotes	those
T8141	6073-6081	VBN	denotes	observed
T8142	6082-6085	IN	denotes	for
T8143	6086-6091	NN	denotes	Snail
T8144	6092-6099	NN	denotes	protein
T8145	6100-6109	NN	denotes	induction
T8146	6109-6110	.	denotes	.
T8147	6110-6382	sentence	denotes	Moreover, the stimulatory effects appeared to be specific to TGF-β2, since they were abrogated either by heat inactivation of the TGF-β2 protein or by mutation of a putative SMAD binding element located about 1.8 kb 5′ from the Snail transcription start site (Figure 5D).
T8148	6111-6119	RB	denotes	Moreover
T8150	6119-6121	,	denotes	,
T8151	6121-6124	DT	denotes	the
T8153	6125-6136	JJ	denotes	stimulatory
T8152	6137-6144	NNS	denotes	effects
T8149	6145-6153	VBD	denotes	appeared
T8154	6154-6156	TO	denotes	to
T8155	6157-6159	VB	denotes	be
T8156	6160-6168	JJ	denotes	specific
T8157	6169-6171	IN	denotes	to
T8158	6172-6175	NN	denotes	TGF
T8160	6175-6176	HYPH	denotes	-
T8159	6176-6178	NN	denotes	β2
T8161	6178-6180	,	denotes	,
T8162	6180-6185	IN	denotes	since
T8164	6186-6190	PRP	denotes	they
T8165	6191-6195	VBD	denotes	were
T8163	6196-6205	VBN	denotes	abrogated
T8166	6206-6212	CC	denotes	either
T8167	6213-6215	IN	denotes	by
T8168	6216-6220	NN	denotes	heat
T8169	6221-6233	NN	denotes	inactivation
T8170	6234-6236	IN	denotes	of
T8171	6237-6240	DT	denotes	the
T8173	6241-6244	NN	denotes	TGF
T8175	6244-6245	HYPH	denotes	-
T8174	6245-6247	NN	denotes	β2
T8172	6248-6255	NN	denotes	protein
T8176	6256-6258	CC	denotes	or
T8177	6259-6261	IN	denotes	by
T8178	6262-6270	NN	denotes	mutation
T8179	6271-6273	IN	denotes	of
T8180	6274-6275	DT	denotes	a
T8182	6276-6284	JJ	denotes	putative
T8183	6285-6289	NN	denotes	SMAD
T8184	6290-6297	NN	denotes	binding
T8181	6298-6305	NN	denotes	element
T8185	6306-6313	VBN	denotes	located
T8186	6314-6319	IN	denotes	about
T8187	6320-6323	CD	denotes	1.8
T8188	6324-6326	NN	denotes	kb
T8189	6327-6328	CD	denotes	5
T8190	6328-6329	SYM	denotes	′
T8191	6330-6334	IN	denotes	from
T8192	6335-6338	DT	denotes	the
T8194	6339-6344	NN	denotes	Snail
T8195	6345-6358	NN	denotes	transcription
T8196	6359-6364	NN	denotes	start
T8193	6365-6369	NN	denotes	site
T8197	6370-6371	-LRB-	denotes	(
T8199	6371-6377	NN	denotes	Figure
T8198	6378-6380	NN	denotes	5D
T8200	6380-6381	-RRB-	denotes	)
T8201	6381-6382	.	denotes	.
T8202	6382-6621	sentence	denotes	Taken together, these results suggested that in keratinocytes, TGF-β2 signaling results in a pSMAD2-dependent transient activation of the Snail gene, and that maintenance of Snail protein relies, in part, upon sustained promoter activity.
T8203	6383-6388	VBN	denotes	Taken
T8205	6389-6397	RB	denotes	together
T8206	6397-6399	,	denotes	,
T8207	6399-6404	DT	denotes	these
T8208	6405-6412	NNS	denotes	results
T8204	6413-6422	VBD	denotes	suggested
T8209	6423-6427	IN	denotes	that
T8211	6428-6430	IN	denotes	in
T8212	6431-6444	NNS	denotes	keratinocytes
T8213	6444-6446	,	denotes	,
T8214	6446-6449	NN	denotes	TGF
T8216	6449-6450	HYPH	denotes	-
T8215	6450-6452	NN	denotes	β2
T8217	6453-6462	NN	denotes	signaling
T8210	6463-6470	VBZ	denotes	results
T8218	6471-6473	IN	denotes	in
T8219	6474-6475	DT	denotes	a
T8221	6476-6482	NN	denotes	pSMAD2
T8223	6482-6483	HYPH	denotes	-
T8222	6483-6492	JJ	denotes	dependent
T8224	6493-6502	JJ	denotes	transient
T8220	6503-6513	NN	denotes	activation
T8225	6514-6516	IN	denotes	of
T8226	6517-6520	DT	denotes	the
T8228	6521-6526	NN	denotes	Snail
T8227	6527-6531	NN	denotes	gene
T8229	6531-6533	,	denotes	,
T8230	6533-6536	CC	denotes	and
T8231	6537-6541	IN	denotes	that
T8233	6542-6553	NN	denotes	maintenance
T8234	6554-6556	IN	denotes	of
T8235	6557-6562	NN	denotes	Snail
T8236	6563-6570	NN	denotes	protein
T8232	6571-6577	VBZ	denotes	relies
T8237	6577-6579	,	denotes	,
T8238	6579-6581	IN	denotes	in
T8239	6582-6586	NN	denotes	part
T8240	6586-6588	,	denotes	,
T8241	6588-6592	IN	denotes	upon
T8242	6593-6602	JJ	denotes	sustained
T8244	6603-6611	NN	denotes	promoter
T8243	6612-6620	NN	denotes	activity
T8245	6620-6621	.	denotes	.
T8246	6621-6844	sentence	denotes	The brevity of Snail gene and protein induction in TGF-β2 treated cultured keratinocytes resembled the temporal appearance of Snail mRNA and protein at the initiation of hair follicle morphogenesis in embryonic mouse skin.
T8247	6622-6625	DT	denotes	The
T8248	6626-6633	NN	denotes	brevity
T8250	6634-6636	IN	denotes	of
T8251	6637-6642	NN	denotes	Snail
T8252	6643-6647	NN	denotes	gene
T8254	6648-6651	CC	denotes	and
T8255	6652-6659	NN	denotes	protein
T8253	6660-6669	NN	denotes	induction
T8256	6670-6672	IN	denotes	in
T8257	6673-6676	NN	denotes	TGF
T8259	6676-6677	HYPH	denotes	-
T8258	6677-6679	NN	denotes	β2
T8260	6680-6687	VBN	denotes	treated
T8262	6688-6696	VBN	denotes	cultured
T8261	6697-6710	NNS	denotes	keratinocytes
T8249	6711-6720	VBD	denotes	resembled
T8263	6721-6724	DT	denotes	the
T8265	6725-6733	JJ	denotes	temporal
T8264	6734-6744	NN	denotes	appearance
T8266	6745-6747	IN	denotes	of
T8267	6748-6753	NN	denotes	Snail
T8268	6754-6758	NN	denotes	mRNA
T8269	6759-6762	CC	denotes	and
T8270	6763-6770	NN	denotes	protein
T8271	6771-6773	IN	denotes	at
T8272	6774-6777	DT	denotes	the
T8273	6778-6788	NN	denotes	initiation
T8274	6789-6791	IN	denotes	of
T8275	6792-6796	NN	denotes	hair
T8276	6797-6805	NN	denotes	follicle
T8277	6806-6819	NN	denotes	morphogenesis
T8278	6820-6822	IN	denotes	in
T8279	6823-6832	JJ	denotes	embryonic
T8281	6833-6838	NN	denotes	mouse
T8280	6839-6843	NN	denotes	skin
T8282	6843-6844	.	denotes	.
T8283	6844-7010	sentence	denotes	To test whether TGF-β2 might be required for Snail induction in hair bud formation in vivo, we first analyzed whether TGF-β2 was expressed in or around the hair bud.
T8284	6845-6847	TO	denotes	To
T8285	6848-6852	VB	denotes	test
T8287	6853-6860	IN	denotes	whether
T8289	6861-6864	NN	denotes	TGF
T8291	6864-6865	HYPH	denotes	-
T8290	6865-6867	NN	denotes	β2
T8292	6868-6873	MD	denotes	might
T8293	6874-6876	VB	denotes	be
T8288	6877-6885	VBN	denotes	required
T8294	6886-6889	IN	denotes	for
T8295	6890-6895	NN	denotes	Snail
T8296	6896-6905	NN	denotes	induction
T8297	6906-6908	IN	denotes	in
T8298	6909-6913	NN	denotes	hair
T8299	6914-6917	NN	denotes	bud
T8300	6918-6927	NN	denotes	formation
T8301	6928-6930	FW	denotes	in
T8302	6931-6935	FW	denotes	vivo
T8303	6935-6937	,	denotes	,
T8304	6937-6939	PRP	denotes	we
T8305	6940-6945	RB	denotes	first
T8286	6946-6954	VBD	denotes	analyzed
T8306	6955-6962	IN	denotes	whether
T8308	6963-6966	NN	denotes	TGF
T8310	6966-6967	HYPH	denotes	-
T8309	6967-6969	NN	denotes	β2
T8311	6970-6973	VBD	denotes	was
T8307	6974-6983	VBN	denotes	expressed
T8312	6984-6986	IN	denotes	in
T8313	6987-6989	CC	denotes	or
T8314	6990-6996	IN	denotes	around
T8315	6997-7000	DT	denotes	the
T8317	7001-7005	NN	denotes	hair
T8316	7006-7009	NN	denotes	bud
T8318	7009-7010	.	denotes	.
T8319	7010-7120	sentence	denotes	Consistent with previous observations [33], an anti-TGF-β2 antibody labeled developing hair buds (Figure 6A).
T8320	7011-7021	JJ	denotes	Consistent
T8322	7022-7026	IN	denotes	with
T8323	7027-7035	JJ	denotes	previous
T8324	7036-7048	NNS	denotes	observations
T8325	7049-7050	-LRB-	denotes	[
T8326	7050-7052	CD	denotes	33
T8327	7052-7053	-RRB-	denotes	]
T8328	7053-7055	,	denotes	,
T8329	7055-7057	DT	denotes	an
T8331	7058-7066	JJ	denotes	anti-TGF
T8333	7066-7067	HYPH	denotes	-
T8332	7067-7069	NN	denotes	β2
T8330	7070-7078	NN	denotes	antibody
T8321	7079-7086	VBD	denotes	labeled
T8334	7087-7097	VBG	denotes	developing
T8336	7098-7102	NN	denotes	hair
T8335	7103-7107	NNS	denotes	buds
T8337	7108-7109	-LRB-	denotes	(
T8339	7109-7115	NN	denotes	Figure
T8338	7116-7118	NN	denotes	6A
T8340	7118-7119	-RRB-	denotes	)
T8341	7119-7120	.	denotes	.
T8342	7120-7276	sentence	denotes	This labeling appeared to be specific as judged by the lack of staining in follicle buds from mice homozygous for a TGF-β2 null mutation (Figure 6A; [34]).
T8343	7121-7125	DT	denotes	This
T8344	7126-7134	NN	denotes	labeling
T8345	7135-7143	VBD	denotes	appeared
T8346	7144-7146	TO	denotes	to
T8347	7147-7149	VB	denotes	be
T8348	7150-7158	JJ	denotes	specific
T8349	7159-7161	IN	denotes	as
T8350	7162-7168	VBN	denotes	judged
T8351	7169-7171	IN	denotes	by
T8352	7172-7175	DT	denotes	the
T8353	7176-7180	NN	denotes	lack
T8354	7181-7183	IN	denotes	of
T8355	7184-7192	NN	denotes	staining
T8356	7193-7195	IN	denotes	in
T8357	7196-7204	NN	denotes	follicle
T8358	7205-7209	NNS	denotes	buds
T8359	7210-7214	IN	denotes	from
T8360	7215-7219	NNS	denotes	mice
T8361	7220-7230	JJ	denotes	homozygous
T8362	7231-7234	IN	denotes	for
T8363	7235-7236	DT	denotes	a
T8365	7237-7240	NN	denotes	TGF
T8367	7240-7241	HYPH	denotes	-
T8366	7241-7243	NN	denotes	β2
T8368	7244-7248	JJ	denotes	null
T8364	7249-7257	NN	denotes	mutation
T8369	7258-7259	-LRB-	denotes	(
T8371	7259-7265	NN	denotes	Figure
T8372	7266-7268	NN	denotes	6A
T8373	7268-7269	:	denotes	;
T8374	7270-7271	-LRB-	denotes	[
T8370	7271-7273	CD	denotes	34
T8375	7273-7274	-RRB-	denotes	]
T8376	7274-7275	-RRB-	denotes	)
T8377	7275-7276	.	denotes	.
T8378	7276-7409	sentence	denotes	Moreover, the downstream effector of TGF-β2 signaling, pSMAD2, was also expressed in WT, but not TGF-β2-null, hair buds (Figure 6B).
T8379	7277-7285	RB	denotes	Moreover
T8381	7285-7287	,	denotes	,
T8382	7287-7290	DT	denotes	the
T8384	7291-7301	JJ	denotes	downstream
T8383	7302-7310	NN	denotes	effector
T8385	7311-7313	IN	denotes	of
T8386	7314-7317	NN	denotes	TGF
T8388	7317-7318	HYPH	denotes	-
T8387	7318-7320	NN	denotes	β2
T8389	7321-7330	NN	denotes	signaling
T8390	7330-7332	,	denotes	,
T8391	7332-7338	NN	denotes	pSMAD2
T8392	7338-7340	,	denotes	,
T8393	7340-7343	VBD	denotes	was
T8394	7344-7348	RB	denotes	also
T8380	7349-7358	VBN	denotes	expressed
T8395	7359-7361	IN	denotes	in
T8396	7362-7364	NN	denotes	WT
T8398	7364-7366	,	denotes	,
T8399	7366-7369	CC	denotes	but
T8400	7370-7373	RB	denotes	not
T8401	7374-7377	NN	denotes	TGF
T8403	7377-7378	HYPH	denotes	-
T8402	7378-7380	NN	denotes	β2
T8405	7380-7381	HYPH	denotes	-
T8404	7381-7385	JJ	denotes	null
T8406	7385-7387	,	denotes	,
T8407	7387-7391	NN	denotes	hair
T8397	7392-7396	NNS	denotes	buds
T8408	7397-7398	-LRB-	denotes	(
T8410	7398-7404	NN	denotes	Figure
T8409	7405-7407	NN	denotes	6B
T8411	7407-7408	-RRB-	denotes	)
T8412	7408-7409	.	denotes	.
T8413	7409-7562	sentence	denotes	Together, these data underscore the importance of the TGF-β2 isoform despite expression of both TGF-β1 and TGF-β2 in developing hair buds at this stage.
T8414	7410-7418	RB	denotes	Together
T8416	7418-7420	,	denotes	,
T8417	7420-7425	DT	denotes	these
T8418	7426-7430	NNS	denotes	data
T8415	7431-7441	VBP	denotes	underscore
T8419	7442-7445	DT	denotes	the
T8420	7446-7456	NN	denotes	importance
T8421	7457-7459	IN	denotes	of
T8422	7460-7463	DT	denotes	the
T8424	7464-7467	NN	denotes	TGF
T8426	7467-7468	HYPH	denotes	-
T8425	7468-7470	NN	denotes	β2
T8423	7471-7478	NN	denotes	isoform
T8427	7479-7486	IN	denotes	despite
T8428	7487-7497	NN	denotes	expression
T8429	7498-7500	IN	denotes	of
T8430	7501-7505	CC	denotes	both
T8432	7506-7509	NN	denotes	TGF
T8433	7509-7510	HYPH	denotes	-
T8431	7510-7512	NN	denotes	β1
T8434	7513-7516	CC	denotes	and
T8435	7517-7520	NN	denotes	TGF
T8437	7520-7521	HYPH	denotes	-
T8436	7521-7523	NN	denotes	β2
T8438	7524-7526	IN	denotes	in
T8439	7527-7537	VBG	denotes	developing
T8441	7538-7542	NN	denotes	hair
T8440	7543-7547	NNS	denotes	buds
T8442	7548-7550	IN	denotes	at
T8443	7551-7555	DT	denotes	this
T8444	7556-7561	NN	denotes	stage
T8445	7561-7562	.	denotes	.
T8446	7562-8693	sentence	denotes	Figure 6 TGF-β2 Is Necessary to Induce Snail Expression and Regulate Proliferation and E-Cadherin in the Hair Bud (A–D) Skins from TGF-β2 WT or KO E17.5 embryos were analyzed for expression of TGF-β2 protein (A), which is present in the epidermis and dermis as previously described [33] and in the hair bud, pSMAD2 (B), Snail (C), and Snail mRNA (D). Arrows point to the hair buds. (E) Western blot analyses of Snail expression in the skins of 2-wk-old K14-Smad2 transgenic (SMAD2 TG) and WT littermate (WT) mice. Antibody to tubulin was used as a control for equal protein loadings. The K14-Smad2 Tg mouse was previously shown to possess activated TGF-β signaling [35]. (F–G) Proliferation markers Ki67 (F) and pMAPK (G) are diminished in TGF-β2-null hair relative to its WT counterpart. (H–J) TGF-β2-null hair fails to down-regulate E-cadherin (H). Wnt and noggin signaling pathways are still intact in the TGF-β2 null hair as nuclear LEF-1 (I) and nuclear β-catenin (J) are still expressed. To further explore the possible relation between Snail and TGF-β2, we examined the status of Snail expression in TGF-β2-null hair buds.
T8447	8558-8560	TO	denotes	To
T8449	8561-8568	RB	denotes	further
T8448	8569-8576	VB	denotes	explore
T8451	8577-8580	DT	denotes	the
T8453	8581-8589	JJ	denotes	possible
T8452	8590-8598	NN	denotes	relation
T8454	8599-8606	IN	denotes	between
T8455	8607-8612	NN	denotes	Snail
T8456	8613-8616	CC	denotes	and
T8457	8617-8620	NN	denotes	TGF
T8459	8620-8621	HYPH	denotes	-
T8458	8621-8623	NN	denotes	β2
T8460	8623-8625	,	denotes	,
T8461	8625-8627	PRP	denotes	we
T8450	8628-8636	VBD	denotes	examined
T8462	8637-8640	DT	denotes	the
T8463	8641-8647	NN	denotes	status
T8464	8648-8650	IN	denotes	of
T8465	8651-8656	NN	denotes	Snail
T8466	8657-8667	NN	denotes	expression
T8467	8668-8670	IN	denotes	in
T8468	8671-8674	NN	denotes	TGF
T8470	8674-8675	HYPH	denotes	-
T8469	8675-8677	NN	denotes	β2
T8472	8677-8678	HYPH	denotes	-
T8471	8678-8682	JJ	denotes	null
T8474	8683-8687	NN	denotes	hair
T8473	8688-8692	NNS	denotes	buds
T8475	8692-8693	.	denotes	.
T8476	8693-8846	sentence	denotes	As judged by immunohistochemistry, Snail protein was absent from E17.5 skin of TGF-β2-null embryos but not from that of control littermates (Figure 6C).
T8477	8694-8696	IN	denotes	As
T8478	8697-8703	VBN	denotes	judged
T8480	8704-8706	IN	denotes	by
T8481	8707-8727	NN	denotes	immunohistochemistry
T8482	8727-8729	,	denotes	,
T8483	8729-8734	NN	denotes	Snail
T8484	8735-8742	NN	denotes	protein
T8479	8743-8746	VBD	denotes	was
T8485	8747-8753	JJ	denotes	absent
T8486	8754-8758	IN	denotes	from
T8487	8759-8764	NN	denotes	E17.5
T8488	8765-8769	NN	denotes	skin
T8489	8770-8772	IN	denotes	of
T8490	8773-8776	NN	denotes	TGF
T8492	8776-8777	HYPH	denotes	-
T8491	8777-8779	NN	denotes	β2
T8494	8779-8780	HYPH	denotes	-
T8493	8780-8784	JJ	denotes	null
T8495	8785-8792	NNS	denotes	embryos
T8496	8793-8796	CC	denotes	but
T8497	8797-8800	RB	denotes	not
T8498	8801-8805	IN	denotes	from
T8499	8806-8810	DT	denotes	that
T8500	8811-8813	IN	denotes	of
T8501	8814-8821	NN	denotes	control
T8502	8822-8833	NNS	denotes	littermates
T8503	8834-8835	-LRB-	denotes	(
T8505	8835-8841	NN	denotes	Figure
T8504	8842-8844	NN	denotes	6C
T8506	8844-8845	-RRB-	denotes	)
T8507	8845-8846	.	denotes	.
T8508	8846-9081	sentence	denotes	This effect appeared to be exerted at the transcriptional level, since Snail mRNAs were also not found in TGF-β2 null hair buds under conditions in which the signal was readily detected in the hair buds of littermate skin (Figure 6D).
T8509	8847-8851	DT	denotes	This
T8510	8852-8858	NN	denotes	effect
T8511	8859-8867	VBD	denotes	appeared
T8512	8868-8870	TO	denotes	to
T8514	8871-8873	VB	denotes	be
T8513	8874-8881	VBN	denotes	exerted
T8515	8882-8884	IN	denotes	at
T8516	8885-8888	DT	denotes	the
T8518	8889-8904	JJ	denotes	transcriptional
T8517	8905-8910	NN	denotes	level
T8519	8910-8912	,	denotes	,
T8520	8912-8917	IN	denotes	since
T8522	8918-8923	NN	denotes	Snail
T8523	8924-8929	NNS	denotes	mRNAs
T8524	8930-8934	VBD	denotes	were
T8525	8935-8939	RB	denotes	also
T8526	8940-8943	RB	denotes	not
T8521	8944-8949	VBN	denotes	found
T8527	8950-8952	IN	denotes	in
T8528	8953-8956	NN	denotes	TGF
T8530	8956-8957	HYPH	denotes	-
T8529	8957-8959	NN	denotes	β2
T8531	8960-8964	JJ	denotes	null
T8533	8965-8969	NN	denotes	hair
T8532	8970-8974	NNS	denotes	buds
T8534	8975-8980	IN	denotes	under
T8535	8981-8991	NNS	denotes	conditions
T8536	8992-8994	IN	denotes	in
T8538	8995-9000	WDT	denotes	which
T8539	9001-9004	DT	denotes	the
T8540	9005-9011	NN	denotes	signal
T8541	9012-9015	VBD	denotes	was
T8542	9016-9023	RB	denotes	readily
T8537	9024-9032	VBN	denotes	detected
T8543	9033-9035	IN	denotes	in
T8544	9036-9039	DT	denotes	the
T8546	9040-9044	NN	denotes	hair
T8545	9045-9049	NNS	denotes	buds
T8547	9050-9052	IN	denotes	of
T8548	9053-9063	NN	denotes	littermate
T8549	9064-9068	NN	denotes	skin
T8550	9069-9070	-LRB-	denotes	(
T8552	9070-9076	NN	denotes	Figure
T8551	9077-9079	NN	denotes	6D
T8553	9079-9080	-RRB-	denotes	)
T8554	9080-9081	.	denotes	.
T8555	9081-9292	sentence	denotes	Conversely, in 2-wk-old K14-Smad2 Tg mice, which display elevated TGF-β signaling in skin [35], Snail protein was readily detected by Western blot analyses, where it was not found in postnatal skin (Figure 6E).
T8556	9082-9092	RB	denotes	Conversely
T8558	9092-9094	,	denotes	,
T8559	9094-9096	IN	denotes	in
T8560	9097-9098	CD	denotes	2
T8562	9098-9099	HYPH	denotes	-
T8561	9099-9101	NN	denotes	wk
T8564	9101-9102	HYPH	denotes	-
T8563	9102-9105	JJ	denotes	old
T8566	9106-9109	NN	denotes	K14
T8568	9109-9110	HYPH	denotes	-
T8567	9110-9115	NN	denotes	Smad2
T8569	9116-9118	NN	denotes	Tg
T8565	9119-9123	NNS	denotes	mice
T8570	9123-9125	,	denotes	,
T8571	9125-9130	WDT	denotes	which
T8572	9131-9138	VBP	denotes	display
T8573	9139-9147	VBN	denotes	elevated
T8575	9148-9151	NN	denotes	TGF
T8577	9151-9152	HYPH	denotes	-
T8576	9152-9153	NN	denotes	β
T8574	9154-9163	NN	denotes	signaling
T8578	9164-9166	IN	denotes	in
T8579	9167-9171	NN	denotes	skin
T8580	9172-9173	-LRB-	denotes	[
T8581	9173-9175	CD	denotes	35
T8582	9175-9176	-RRB-	denotes	]
T8583	9176-9178	,	denotes	,
T8584	9178-9183	NN	denotes	Snail
T8585	9184-9191	NN	denotes	protein
T8586	9192-9195	VBD	denotes	was
T8587	9196-9203	RB	denotes	readily
T8557	9204-9212	VBN	denotes	detected
T8588	9213-9215	IN	denotes	by
T8589	9216-9223	NNP	denotes	Western
T8590	9224-9228	NN	denotes	blot
T8591	9229-9237	NNS	denotes	analyses
T8592	9237-9239	,	denotes	,
T8593	9239-9244	WRB	denotes	where
T8595	9245-9247	PRP	denotes	it
T8596	9248-9251	VBD	denotes	was
T8597	9252-9255	RB	denotes	not
T8594	9256-9261	VBN	denotes	found
T8598	9262-9264	IN	denotes	in
T8599	9265-9274	JJ	denotes	postnatal
T8600	9275-9279	NN	denotes	skin
T8601	9280-9281	-LRB-	denotes	(
T8603	9281-9287	NN	denotes	Figure
T8602	9288-9290	NN	denotes	6E
T8604	9290-9291	-RRB-	denotes	)
T8605	9291-9292	.	denotes	.
T8606	9292-9476	sentence	denotes	Taken together, these results provide compelling evidence that TGF-β2 is functionally important for inducing Snail gene expression in a pSMAD-dependent manner in developing hair buds.
T8607	9293-9298	VBN	denotes	Taken
T8609	9299-9307	RB	denotes	together
T8610	9307-9309	,	denotes	,
T8611	9309-9314	DT	denotes	these
T8612	9315-9322	NNS	denotes	results
T8608	9323-9330	VBP	denotes	provide
T8613	9331-9341	JJ	denotes	compelling
T8614	9342-9350	NN	denotes	evidence
T8615	9351-9355	IN	denotes	that
T8617	9356-9359	NN	denotes	TGF
T8619	9359-9360	HYPH	denotes	-
T8618	9360-9362	NN	denotes	β2
T8616	9363-9365	VBZ	denotes	is
T8620	9366-9378	RB	denotes	functionally
T8621	9379-9388	JJ	denotes	important
T8622	9389-9392	IN	denotes	for
T8623	9393-9401	VBG	denotes	inducing
T8624	9402-9407	NN	denotes	Snail
T8626	9408-9412	NN	denotes	gene
T8625	9413-9423	NN	denotes	expression
T8627	9424-9426	IN	denotes	in
T8628	9427-9428	DT	denotes	a
T8630	9429-9434	NN	denotes	pSMAD
T8632	9434-9435	HYPH	denotes	-
T8631	9435-9444	JJ	denotes	dependent
T8629	9445-9451	NN	denotes	manner
T8633	9452-9454	IN	denotes	in
T8634	9455-9465	VBG	denotes	developing
T8636	9466-9470	NN	denotes	hair
T8635	9471-9475	NNS	denotes	buds
T8637	9475-9476	.	denotes	.
T8638	9476-9580	sentence	denotes	Whether pMARK activity also contributes to Snail induction was not addressed in the present study [15].
T8639	9477-9484	IN	denotes	Whether
T8641	9485-9490	NN	denotes	pMARK
T8642	9491-9499	NN	denotes	activity
T8643	9500-9504	RB	denotes	also
T8640	9505-9516	VBZ	denotes	contributes
T8645	9517-9519	IN	denotes	to
T8646	9520-9525	NN	denotes	Snail
T8647	9526-9535	NN	denotes	induction
T8648	9536-9539	VBD	denotes	was
T8649	9540-9543	RB	denotes	not
T8644	9544-9553	VBN	denotes	addressed
T8650	9554-9556	IN	denotes	in
T8651	9557-9560	DT	denotes	the
T8653	9561-9568	JJ	denotes	present
T8652	9569-9574	NN	denotes	study
T8654	9575-9576	-LRB-	denotes	[
T8655	9576-9578	CD	denotes	15
T8656	9578-9579	-RRB-	denotes	]
T8657	9579-9580	.	denotes	.
T8658	9580-9690	sentence	denotes	Although some hair buds still formed in TGF-β2 null skin, their number was reduced by approximately 50% [32].
T8659	9581-9589	IN	denotes	Although
T8661	9590-9594	DT	denotes	some
T8663	9595-9599	NN	denotes	hair
T8662	9600-9604	NNS	denotes	buds
T8664	9605-9610	RB	denotes	still
T8660	9611-9617	VBD	denotes	formed
T8666	9618-9620	IN	denotes	in
T8667	9621-9624	NN	denotes	TGF
T8669	9624-9625	HYPH	denotes	-
T8668	9625-9627	NN	denotes	β2
T8670	9628-9632	JJ	denotes	null
T8671	9633-9637	NN	denotes	skin
T8672	9637-9639	,	denotes	,
T8673	9639-9644	PRP$	denotes	their
T8674	9645-9651	NN	denotes	number
T8675	9652-9655	VBD	denotes	was
T8665	9656-9663	VBN	denotes	reduced
T8676	9664-9666	IN	denotes	by
T8677	9667-9680	RB	denotes	approximately
T8678	9681-9683	CD	denotes	50
T8679	9683-9684	NN	denotes	%
T8680	9685-9686	-LRB-	denotes	[
T8681	9686-9688	CD	denotes	32
T8682	9688-9689	-RRB-	denotes	]
T8683	9689-9690	.	denotes	.
T8684	9690-9858	sentence	denotes	Thus, although the pathway mediated by TGF-β2 signaling impacts the earliest step of epithelial invagination, it does not appear to be essential for bud morphogenesis.
T8685	9691-9695	RB	denotes	Thus
T8687	9695-9697	,	denotes	,
T8688	9697-9705	IN	denotes	although
T8690	9706-9709	DT	denotes	the
T8691	9710-9717	NN	denotes	pathway
T8692	9718-9726	VBN	denotes	mediated
T8693	9727-9729	IN	denotes	by
T8694	9730-9733	NN	denotes	TGF
T8696	9733-9734	HYPH	denotes	-
T8695	9734-9736	NN	denotes	β2
T8697	9737-9746	NN	denotes	signaling
T8689	9747-9754	VBZ	denotes	impacts
T8698	9755-9758	DT	denotes	the
T8700	9759-9767	JJS	denotes	earliest
T8699	9768-9772	NN	denotes	step
T8701	9773-9775	IN	denotes	of
T8702	9776-9786	JJ	denotes	epithelial
T8703	9787-9799	NN	denotes	invagination
T8704	9799-9801	,	denotes	,
T8705	9801-9803	PRP	denotes	it
T8706	9804-9808	VBZ	denotes	does
T8707	9809-9812	RB	denotes	not
T8686	9813-9819	VB	denotes	appear
T8708	9820-9822	TO	denotes	to
T8709	9823-9825	VB	denotes	be
T8710	9826-9835	JJ	denotes	essential
T8711	9836-9839	IN	denotes	for
T8712	9840-9843	NN	denotes	bud
T8713	9844-9857	NN	denotes	morphogenesis
T8714	9857-9858	.	denotes	.
T8715	9858-10132	sentence	denotes	Consistent with this notion, basement membrane remodeling still took place in the TGF-β2-null buds, as judged by immunofluorescence with antibodies against β4 integrin, an integral component of keratinocyte-mediated adhesion to its underlying basement membrane (Figure 6F).
T8716	9859-9869	JJ	denotes	Consistent
T8718	9870-9874	IN	denotes	with
T8719	9875-9879	DT	denotes	this
T8720	9880-9886	NN	denotes	notion
T8721	9886-9888	,	denotes	,
T8722	9888-9896	NN	denotes	basement
T8723	9897-9905	NN	denotes	membrane
T8724	9906-9916	NN	denotes	remodeling
T8725	9917-9922	RB	denotes	still
T8717	9923-9927	VBD	denotes	took
T8726	9928-9933	RP	denotes	place
T8727	9934-9936	IN	denotes	in
T8728	9937-9940	DT	denotes	the
T8730	9941-9944	NN	denotes	TGF
T8732	9944-9945	HYPH	denotes	-
T8731	9945-9947	NN	denotes	β2
T8734	9947-9948	HYPH	denotes	-
T8733	9948-9952	JJ	denotes	null
T8729	9953-9957	NNS	denotes	buds
T8735	9957-9959	,	denotes	,
T8736	9959-9961	IN	denotes	as
T8737	9962-9968	VBN	denotes	judged
T8738	9969-9971	IN	denotes	by
T8739	9972-9990	NN	denotes	immunofluorescence
T8740	9991-9995	IN	denotes	with
T8741	9996-10006	NNS	denotes	antibodies
T8742	10007-10014	IN	denotes	against
T8743	10015-10017	NN	denotes	β4
T8744	10018-10026	NN	denotes	integrin
T8745	10026-10028	,	denotes	,
T8746	10028-10030	DT	denotes	an
T8748	10031-10039	JJ	denotes	integral
T8747	10040-10049	NN	denotes	component
T8749	10050-10052	IN	denotes	of
T8750	10053-10065	NN	denotes	keratinocyte
T8752	10065-10066	HYPH	denotes	-
T8751	10066-10074	VBN	denotes	mediated
T8753	10075-10083	NN	denotes	adhesion
T8754	10084-10086	IN	denotes	to
T8755	10087-10090	PRP$	denotes	its
T8757	10091-10101	JJ	denotes	underlying
T8758	10102-10110	NN	denotes	basement
T8756	10111-10119	NN	denotes	membrane
T8759	10120-10121	-LRB-	denotes	(
T8761	10121-10127	NN	denotes	Figure
T8760	10128-10130	NN	denotes	6F
T8762	10130-10131	-RRB-	denotes	)
T8763	10131-10132	.	denotes	.
T8764	10132-10343	sentence	denotes	In contrast, TGF-β2 signaling appeared to be an important factor for the early proliferation that occurs in the developing hair buds, as judged by anti-Ki67 and anti-pMAPK immunofluorescence (Figure 6F and 6G).
T8765	10133-10135	IN	denotes	In
T8767	10136-10144	NN	denotes	contrast
T8768	10144-10146	,	denotes	,
T8769	10146-10149	NN	denotes	TGF
T8771	10149-10150	HYPH	denotes	-
T8770	10150-10152	NN	denotes	β2
T8772	10153-10162	NN	denotes	signaling
T8766	10163-10171	VBD	denotes	appeared
T8773	10172-10174	TO	denotes	to
T8774	10175-10177	VB	denotes	be
T8775	10178-10180	DT	denotes	an
T8777	10181-10190	JJ	denotes	important
T8776	10191-10197	NN	denotes	factor
T8778	10198-10201	IN	denotes	for
T8779	10202-10205	DT	denotes	the
T8781	10206-10211	JJ	denotes	early
T8780	10212-10225	NN	denotes	proliferation
T8782	10226-10230	WDT	denotes	that
T8783	10231-10237	VBZ	denotes	occurs
T8784	10238-10240	IN	denotes	in
T8785	10241-10244	DT	denotes	the
T8787	10245-10255	VBG	denotes	developing
T8788	10256-10260	NN	denotes	hair
T8786	10261-10265	NNS	denotes	buds
T8789	10265-10267	,	denotes	,
T8790	10267-10269	IN	denotes	as
T8791	10270-10276	VBN	denotes	judged
T8792	10277-10279	IN	denotes	by
T8793	10280-10289	JJ	denotes	anti-Ki67
T8795	10290-10293	CC	denotes	and
T8796	10294-10304	JJ	denotes	anti-pMAPK
T8794	10305-10323	NN	denotes	immunofluorescence
T8797	10324-10325	-LRB-	denotes	(
T8799	10325-10331	NN	denotes	Figure
T8798	10332-10334	NN	denotes	6F
T8800	10335-10338	CC	denotes	and
T8801	10339-10341	NN	denotes	6G
T8802	10341-10342	-RRB-	denotes	)
T8803	10342-10343	.	denotes	.
T8804	10343-10515	sentence	denotes	If TGF-β2 stimulates Snail expression in developing buds, loss of this morphogen would be expected to affect the expression of genes that are typically repressed by Snail.
T8805	10344-10346	IN	denotes	If
T8807	10347-10350	NN	denotes	TGF
T8809	10350-10351	HYPH	denotes	-
T8808	10351-10353	NN	denotes	β2
T8806	10354-10364	VBZ	denotes	stimulates
T8811	10365-10370	NN	denotes	Snail
T8812	10371-10381	NN	denotes	expression
T8813	10382-10384	IN	denotes	in
T8814	10385-10395	VBG	denotes	developing
T8815	10396-10400	NNS	denotes	buds
T8816	10400-10402	,	denotes	,
T8817	10402-10406	NN	denotes	loss
T8818	10407-10409	IN	denotes	of
T8819	10410-10414	DT	denotes	this
T8820	10415-10424	NN	denotes	morphogen
T8821	10425-10430	MD	denotes	would
T8822	10431-10433	VB	denotes	be
T8810	10434-10442	VBN	denotes	expected
T8823	10443-10445	TO	denotes	to
T8824	10446-10452	VB	denotes	affect
T8825	10453-10456	DT	denotes	the
T8826	10457-10467	NN	denotes	expression
T8827	10468-10470	IN	denotes	of
T8828	10471-10476	NNS	denotes	genes
T8829	10477-10481	WDT	denotes	that
T8831	10482-10485	VBP	denotes	are
T8832	10486-10495	RB	denotes	typically
T8830	10496-10505	VBN	denotes	repressed
T8833	10506-10508	IN	denotes	by
T8834	10509-10514	NN	denotes	Snail
T8835	10514-10515	.	denotes	.
T8836	10515-10660	sentence	denotes	Since a major target for Snail-mediated repression is the E-cadherin gene [12,13], we investigated the status of E-cadherin in TGF-β2-null buds.
T8837	10516-10521	IN	denotes	Since
T8839	10522-10523	DT	denotes	a
T8841	10524-10529	JJ	denotes	major
T8840	10530-10536	NN	denotes	target
T8842	10537-10540	IN	denotes	for
T8843	10541-10546	NN	denotes	Snail
T8845	10546-10547	HYPH	denotes	-
T8844	10547-10555	VBN	denotes	mediated
T8846	10556-10566	NN	denotes	repression
T8838	10567-10569	VBZ	denotes	is
T8848	10570-10573	DT	denotes	the
T8850	10574-10575	NN	denotes	E
T8852	10575-10576	HYPH	denotes	-
T8851	10576-10584	NN	denotes	cadherin
T8849	10585-10589	NN	denotes	gene
T8853	10590-10591	-LRB-	denotes	[
T8855	10591-10593	CD	denotes	12
T8856	10593-10594	,	denotes	,
T8854	10594-10596	CD	denotes	13
T8857	10596-10597	-RRB-	denotes	]
T8858	10597-10599	,	denotes	,
T8859	10599-10601	PRP	denotes	we
T8847	10602-10614	VBD	denotes	investigated
T8860	10615-10618	DT	denotes	the
T8861	10619-10625	NN	denotes	status
T8862	10626-10628	IN	denotes	of
T8863	10629-10630	NN	denotes	E
T8865	10630-10631	HYPH	denotes	-
T8864	10631-10639	NN	denotes	cadherin
T8866	10640-10642	IN	denotes	in
T8867	10643-10646	NN	denotes	TGF
T8869	10646-10647	HYPH	denotes	-
T8868	10647-10649	NN	denotes	β2
T8871	10649-10650	HYPH	denotes	-
T8870	10650-10654	JJ	denotes	null
T8872	10655-10659	NNS	denotes	buds
T8873	10659-10660	.	denotes	.
T8874	10660-10795	sentence	denotes	As shown in Figure 6H, hair buds in TGF-β2 null skin displayed elevated immunofluorescence staining relative to their WT counterparts.
T8875	10661-10663	IN	denotes	As
T8876	10664-10669	VBN	denotes	shown
T8878	10670-10672	IN	denotes	in
T8879	10673-10679	NN	denotes	Figure
T8880	10680-10682	NN	denotes	6H
T8881	10682-10684	,	denotes	,
T8882	10684-10688	NN	denotes	hair
T8883	10689-10693	NNS	denotes	buds
T8884	10694-10696	IN	denotes	in
T8885	10697-10700	NN	denotes	TGF
T8887	10700-10701	HYPH	denotes	-
T8886	10701-10703	NN	denotes	β2
T8888	10704-10708	JJ	denotes	null
T8889	10709-10713	NN	denotes	skin
T8877	10714-10723	VBD	denotes	displayed
T8890	10724-10732	JJ	denotes	elevated
T8892	10733-10751	NN	denotes	immunofluorescence
T8891	10752-10760	NN	denotes	staining
T8893	10761-10769	JJ	denotes	relative
T8894	10770-10772	IN	denotes	to
T8895	10773-10778	PRP$	denotes	their
T8897	10779-10781	NN	denotes	WT
T8896	10782-10794	NNS	denotes	counterparts
T8898	10794-10795	.	denotes	.
T8899	10795-11044	sentence	denotes	Previously we demonstrated that the concerted action of the extracellular signals Wnt and noggin are required for the generation of a LEF-1/β-catenin transcription complex to repress E-cadherin transcription at the onset of hair fate specification.
T8900	10796-10806	RB	denotes	Previously
T8902	10807-10809	PRP	denotes	we
T8901	10810-10822	VBD	denotes	demonstrated
T8903	10823-10827	IN	denotes	that
T8905	10828-10831	DT	denotes	the
T8907	10832-10841	JJ	denotes	concerted
T8906	10842-10848	NN	denotes	action
T8908	10849-10851	IN	denotes	of
T8909	10852-10855	DT	denotes	the
T8911	10856-10869	JJ	denotes	extracellular
T8910	10870-10877	NNS	denotes	signals
T8912	10878-10881	NN	denotes	Wnt
T8913	10882-10885	CC	denotes	and
T8914	10886-10892	NN	denotes	noggin
T8915	10893-10896	VBP	denotes	are
T8904	10897-10905	VBN	denotes	required
T8916	10906-10909	IN	denotes	for
T8918	10910-10913	DT	denotes	the
T8919	10914-10924	NN	denotes	generation
T8920	10925-10927	IN	denotes	of
T8921	10928-10929	DT	denotes	a
T8923	10930-10933	NN	denotes	LEF
T8924	10933-10934	HYPH	denotes	-
T8925	10934-10935	CD	denotes	1
T8926	10935-10936	HYPH	denotes	/
T8927	10936-10937	NN	denotes	β
T8929	10937-10938	HYPH	denotes	-
T8928	10938-10945	NN	denotes	catenin
T8930	10946-10959	NN	denotes	transcription
T8922	10960-10967	NN	denotes	complex
T8931	10968-10970	TO	denotes	to
T8917	10971-10978	VB	denotes	repress
T8932	10979-10980	NN	denotes	E
T8934	10980-10981	HYPH	denotes	-
T8933	10981-10989	NN	denotes	cadherin
T8935	10990-11003	NN	denotes	transcription
T8936	11004-11006	IN	denotes	at
T8937	11007-11010	DT	denotes	the
T8938	11011-11016	NN	denotes	onset
T8939	11017-11019	IN	denotes	of
T8940	11020-11024	NN	denotes	hair
T8941	11025-11029	NN	denotes	fate
T8942	11030-11043	NN	denotes	specification
T8943	11043-11044	.	denotes	.
T8944	11044-11211	sentence	denotes	As shown in Figure 6I and 6J, both WT and TGF-β2 null buds exhibited nuclear LEF-1 and β-catenin localization, signs that the Wnt-noggin signaling pathway was intact.
T8945	11045-11047	IN	denotes	As
T8946	11048-11053	VBN	denotes	shown
T8948	11054-11056	IN	denotes	in
T8949	11057-11063	NN	denotes	Figure
T8950	11064-11066	NN	denotes	6I
T8951	11067-11070	CC	denotes	and
T8952	11071-11073	NN	denotes	6J
T8953	11073-11075	,	denotes	,
T8954	11075-11079	CC	denotes	both
T8955	11080-11082	NN	denotes	WT
T8957	11083-11086	CC	denotes	and
T8958	11087-11090	NN	denotes	TGF
T8960	11090-11091	HYPH	denotes	-
T8959	11091-11093	NN	denotes	β2
T8956	11094-11098	JJ	denotes	null
T8961	11099-11103	NNS	denotes	buds
T8947	11104-11113	VBD	denotes	exhibited
T8962	11114-11121	JJ	denotes	nuclear
T8964	11122-11125	NN	denotes	LEF
T8965	11125-11126	HYPH	denotes	-
T8966	11126-11127	CD	denotes	1
T8967	11128-11131	CC	denotes	and
T8968	11132-11133	NN	denotes	β
T8970	11133-11134	HYPH	denotes	-
T8969	11134-11141	NN	denotes	catenin
T8963	11142-11154	NN	denotes	localization
T8971	11154-11156	,	denotes	,
T8972	11156-11161	NNS	denotes	signs
T8973	11162-11166	IN	denotes	that
T8975	11167-11170	DT	denotes	the
T8977	11171-11174	NN	denotes	Wnt
T8979	11174-11175	HYPH	denotes	-
T8978	11175-11181	NN	denotes	noggin
T8980	11182-11191	NN	denotes	signaling
T8976	11192-11199	NN	denotes	pathway
T8974	11200-11203	VBD	denotes	was
T8981	11204-11210	JJ	denotes	intact
T8982	11210-11211	.	denotes	.
T8983	11211-11352	sentence	denotes	These data suggest that during hair follicle morphogenesis, TGF-β2 functions subsequently to Wnt/noggin-mediated determination of hair fate.
T8984	11212-11217	DT	denotes	These
T8985	11218-11222	NNS	denotes	data
T8986	11223-11230	VBP	denotes	suggest
T8987	11231-11235	IN	denotes	that
T8989	11236-11242	IN	denotes	during
T8990	11243-11247	NN	denotes	hair
T8991	11248-11256	NN	denotes	follicle
T8992	11257-11270	NN	denotes	morphogenesis
T8993	11270-11272	,	denotes	,
T8994	11272-11275	NN	denotes	TGF
T8996	11275-11276	HYPH	denotes	-
T8995	11276-11278	NN	denotes	β2
T8988	11279-11288	VBZ	denotes	functions
T8997	11289-11301	RB	denotes	subsequently
T8998	11302-11304	IN	denotes	to
T8999	11305-11308	NN	denotes	Wnt
T9001	11308-11309	HYPH	denotes	/
T9000	11309-11315	NN	denotes	noggin
T9003	11315-11316	HYPH	denotes	-
T9002	11316-11324	VBN	denotes	mediated
T9004	11325-11338	NN	denotes	determination
T9005	11339-11341	IN	denotes	of
T9006	11342-11346	NN	denotes	hair
T9007	11347-11351	NN	denotes	fate
T9008	11351-11352	.	denotes	.
T9009	11352-11570	sentence	denotes	Moreover, through activation of Snail gene expression, TGF-β2 appears to work in tandem with these other morphogens to down-regulate E-cadherin levels, which contributes to the activation of proliferative circuitries.
T9010	11353-11361	RB	denotes	Moreover
T9012	11361-11363	,	denotes	,
T9013	11363-11370	IN	denotes	through
T9014	11371-11381	NN	denotes	activation
T9015	11382-11384	IN	denotes	of
T9016	11385-11390	NN	denotes	Snail
T9018	11391-11395	NN	denotes	gene
T9017	11396-11406	NN	denotes	expression
T9019	11406-11408	,	denotes	,
T9020	11408-11411	NN	denotes	TGF
T9022	11411-11412	HYPH	denotes	-
T9021	11412-11414	NN	denotes	β2
T9011	11415-11422	VBZ	denotes	appears
T9023	11423-11425	TO	denotes	to
T9024	11426-11430	VB	denotes	work
T9025	11431-11433	IN	denotes	in
T9026	11434-11440	NN	denotes	tandem
T9027	11441-11445	IN	denotes	with
T9028	11446-11451	DT	denotes	these
T9030	11452-11457	JJ	denotes	other
T9029	11458-11468	NNS	denotes	morphogens
T9031	11469-11471	TO	denotes	to
T9033	11472-11476	RB	denotes	down
T9034	11476-11477	HYPH	denotes	-
T9032	11477-11485	VB	denotes	regulate
T9035	11486-11487	NN	denotes	E
T9037	11487-11488	HYPH	denotes	-
T9036	11488-11496	NN	denotes	cadherin
T9038	11497-11503	NNS	denotes	levels
T9039	11503-11505	,	denotes	,
T9040	11505-11510	WDT	denotes	which
T9041	11511-11522	VBZ	denotes	contributes
T9042	11523-11525	IN	denotes	to
T9043	11526-11529	DT	denotes	the
T9044	11530-11540	NN	denotes	activation
T9045	11541-11543	IN	denotes	of
T9046	11544-11557	JJ	denotes	proliferative
T9047	11558-11569	NNS	denotes	circuitries
T9048	11569-11570	.	denotes	.
R4172	T7354	T7355	csubj	Probing,Reveals
R4173	T7356	T7357	det	the,Regulation
R4174	T7357	T7354	dobj	Regulation,Probing
R4175	T7358	T7357	prep	of,Regulation
R4176	T7359	T7360	compound	Snail,Gene
R4177	T7360	T7361	compound	Gene,Expression
R4178	T7361	T7358	pobj	Expression,of
R4179	T7362	T7363	det	an,Link
R4180	T7363	T7355	dobj	Link,Reveals
R4181	T7364	T7363	amod	Essential,Link
R4182	T7365	T7363	prep	to,Link
R4183	T7366	T7367	compound	TGF,β2
R4184	T7367	T7369	compound	β2,Signaling
R4185	T7368	T7367	punct	-,β2
R4186	T7369	T7365	pobj	Signaling,to
R4187	T7370	T7355	prep	in,Reveals
R4188	T7371	T7372	det	the,Bud
R4189	T7372	T7370	pobj	Bud,in
R4190	T7373	T7372	amod	Developing,Bud
R4191	T7374	T7372	compound	Hair,Bud
R4192	T7376	T7377	det	The,spike
R4193	T7377	T7379	nsubj	spike,prompted
R4194	T7378	T7377	amod	temporal,spike
R4195	T7380	T7377	prep	of,spike
R4196	T7381	T7382	compound	Snail,expression
R4197	T7382	T7380	pobj	expression,of
R4198	T7383	T7382	compound	mRNA,expression
R4199	T7384	T7377	prep	in,spike
R4200	T7385	T7386	det	the,bud
R4201	T7386	T7384	pobj	bud,in
R4202	T7387	T7386	compound	hair,bud
R4203	T7388	T7379	dobj	us,prompted
R4204	T7389	T7390	aux	to,consider
R4205	T7390	T7379	xcomp	consider,prompted
R4206	T7391	T7392	det	what,factor
R4207	T7392	T7393	dep	factor,regulating
R4208	T7393	T7390	ccomp	regulating,consider
R4209	T7394	T7392	punct	(,factor
R4210	T7395	T7392	nmod	s,factor
R4211	T7396	T7392	punct	),factor
R4212	T7397	T7393	aux	may,regulating
R4213	T7398	T7393	aux	be,regulating
R4214	T7399	T7400	det	the,gene
R4215	T7400	T7393	dobj	gene,regulating
R4216	T7401	T7400	compound	Snail,gene
R4217	T7402	T7379	punct	.,prompted
R4218	T7404	T7405	det	A,variety
R4219	T7405	T7406	nsubj	variety,have
R4220	T7407	T7405	prep	of,variety
R4221	T7408	T7409	amod	extracellular,signals
R4222	T7409	T7407	pobj	signals,of
R4223	T7410	T7411	det	an,impact
R4224	T7411	T7406	dobj	impact,have
R4225	T7412	T7411	prep	on,impact
R4226	T7413	T7414	det	the,expression
R4227	T7414	T7412	pobj	expression,on
R4228	T7415	T7416	compound	cell,type
R4229	T7416	T7417	npadvmod	type,specific
R4230	T7417	T7414	amod	specific,expression
R4231	T7418	T7417	punct	-,specific
R4232	T7419	T7414	prep	of,expression
R4233	T7420	T7421	amod	different,members
R4234	T7421	T7419	pobj	members,of
R4235	T7422	T7423	compound	Snail,family
R4236	T7423	T7421	compound	family,members
R4237	T7424	T7406	punct	", ",have
R4238	T7425	T7406	cc	and,have
R4239	T7426	T7427	nsubj	many,affect
R4240	T7427	T7406	conj	affect,have
R4241	T7428	T7426	prep	of,many
R4242	T7429	T7428	pobj	them,of
R4243	T7430	T7426	punct	", ",many
R4244	T7431	T7426	prep	including,many
R4245	T7432	T7431	pobj	Wnts,including
R4246	T7433	T7432	punct	", ",Wnts
R4247	T7434	T7432	conj	BMPs,Wnts
R4248	T7435	T7434	punct	", ",BMPs
R4249	T7436	T7434	conj	FGFs,BMPs
R4250	T7437	T7436	punct	", ",FGFs
R4251	T7438	T7436	cc	and,FGFs
R4252	T7439	T7440	compound	TGF,βs
R4253	T7440	T7436	conj	βs,FGFs
R4254	T7441	T7440	punct	-,βs
R4255	T7442	T7427	punct	", ",affect
R4256	T7443	T7427	advmod	also,affect
R4257	T7444	T7445	compound	hair,bud
R4258	T7445	T7446	compound	bud,development
R4259	T7446	T7427	dobj	development,affect
R4260	T7447	T7448	punct	[,28
R4261	T7448	T7427	parataxis	28,affect
R4262	T7449	T7448	nummod	2,28
R4263	T7450	T7448	punct	",",28
R4264	T7451	T7448	nummod	16,28
R4265	T7452	T7448	punct	",",28
R4266	T7453	T7448	punct	],28
R4267	T7454	T7427	punct	.,affect
R4268	T7456	T7457	mark	Since,expressed
R4269	T7457	T7461	advcl	expressed,focused
R4270	T7458	T7457	nsubjpass	Snail,expressed
R4271	T7459	T7457	auxpass	is,expressed
R4272	T7460	T7457	neg	not,expressed
R4273	T7462	T7457	prep	in,expressed
R4274	T7463	T7464	amod	cultured,keratinocytes
R4275	T7464	T7462	pobj	keratinocytes,in
R4276	T7465	T7464	compound	skin,keratinocytes
R4277	T7466	T7467	dep	that,secrete
R4278	T7467	T7464	relcl	secrete,keratinocytes
R4279	T7468	T7469	amod	active,BMPs
R4280	T7469	T7467	dobj	BMPs,secrete
R4281	T7470	T7469	cc	and,BMPs
R4282	T7471	T7469	conj	FGFs,BMPs
R4283	T7472	T7473	punct	(,see
R4284	T7473	T7457	parataxis	see,expressed
R4285	T7474	T7475	compound	Figure,1B
R4286	T7475	T7473	dobj	1B,see
R4287	T7476	T7473	punct	),see
R4288	T7477	T7461	punct	", ",focused
R4289	T7478	T7461	nsubj	we,focused
R4290	T7479	T7480	poss	our,attention
R4291	T7480	T7461	dobj	attention,focused
R4292	T7481	T7461	prep	on,focused
R4293	T7482	T7483	nmod	Wnt,signaling
R4294	T7483	T7481	pobj	signaling,on
R4295	T7484	T7482	cc	and,Wnt
R4296	T7485	T7486	compound	TGF,β
R4297	T7486	T7482	conj	β,Wnt
R4298	T7487	T7486	punct	-,β
R4299	T7488	T7483	prep	as,signaling
R4300	T7489	T7490	advmod	more,likely
R4301	T7490	T7491	amod	likely,candidates
R4302	T7491	T7488	pobj	candidates,as
R4303	T7492	T7491	prep	for,candidates
R4304	T7493	T7494	compound	Snail,induction
R4305	T7494	T7492	pobj	induction,for
R4306	T7495	T7491	prep	in,candidates
R4307	T7496	T7497	det	this,type
R4308	T7497	T7495	pobj	type,in
R4309	T7498	T7497	compound	cell,type
R4310	T7499	T7461	punct	.,focused
R4311	T7501	T7502	advmod	Previously,showed
R4312	T7503	T7502	punct	", ",showed
R4313	T7504	T7502	nsubj	we,showed
R4314	T7505	T7506	mark	that,achieved
R4315	T7506	T7502	ccomp	achieved,showed
R4316	T7507	T7508	amod	effective,transmission
R4317	T7508	T7506	nsubjpass	transmission,achieved
R4318	T7509	T7508	prep	of,transmission
R4319	T7510	T7511	det	a,signal
R4320	T7511	T7509	pobj	signal,of
R4321	T7512	T7511	nmod	Wnt,signal
R4322	T7513	T7512	punct	-,Wnt
R4323	T7514	T7512	nummod	3a,Wnt
R4324	T7515	T7508	prep	in,transmission
R4325	T7516	T7517	amod	cultured,keratinocytes
R4326	T7517	T7515	pobj	keratinocytes,in
R4327	T7518	T7506	aux	can,achieved
R4328	T7519	T7506	auxpass	be,achieved
R4329	T7520	T7506	prep	through,achieved
R4330	T7521	T7522	poss	their,exposure
R4331	T7522	T7520	pobj	exposure,through
R4332	T7523	T7522	prep	to,exposure
R4333	T7524	T7525	det	the,inhibitor
R4334	T7525	T7523	pobj	inhibitor,to
R4335	T7526	T7525	compound	BMP,inhibitor
R4336	T7527	T7525	appos	noggin,inhibitor
R4337	T7528	T7525	punct	", ",inhibitor
R4338	T7529	T7530	dep	which,induces
R4339	T7530	T7525	relcl	induces,inhibitor
R4340	T7531	T7532	nmod	LEF,expression
R4341	T7532	T7530	dobj	expression,induces
R4342	T7533	T7531	punct	-,LEF
R4343	T7534	T7531	nummod	1,LEF
R4344	T7535	T7536	punct	[,4
R4345	T7536	T7502	parataxis	4,showed
R4346	T7537	T7536	punct	],4
R4347	T7538	T7502	punct	.,showed
R4348	T7540	T7541	advmod	In,vitro
R4349	T7541	T7542	advmod	vitro,regulated
R4350	T7543	T7542	punct	", ",regulated
R4351	T7544	T7545	det	these,conditions
R4352	T7545	T7542	nsubj	conditions,regulated
R4353	T7546	T7542	advmod	down,regulated
R4354	T7547	T7542	punct	-,regulated
R4355	T7548	T7549	det	the,promoter
R4356	T7549	T7542	dobj	promoter,regulated
R4357	T7550	T7551	compound	E,cadherin
R4358	T7551	T7549	compound	cadherin,promoter
R4359	T7552	T7551	punct	-,cadherin
R4360	T7553	T7542	cc	and,regulated
R4361	T7554	T7542	conj	induced,regulated
R4362	T7555	T7556	det	a,gene
R4363	T7556	T7554	dobj	gene,induced
R4364	T7557	T7558	npadvmod	LEF,sensitive
R4365	T7558	T7556	amod	sensitive,gene
R4366	T7559	T7557	punct	-,LEF
R4367	T7560	T7557	nummod	1,LEF
R4368	T7561	T7557	punct	/,LEF
R4369	T7562	T7563	compound	β,catenin
R4370	T7563	T7557	appos	catenin,LEF
R4371	T7564	T7563	punct	-,catenin
R4372	T7565	T7558	punct	-,sensitive
R4373	T7566	T7556	compound	reporter,gene
R4374	T7567	T7556	punct	", ",gene
R4375	T7568	T7556	appos	TOPFLASH,gene
R4376	T7569	T7570	punct	[,4
R4377	T7570	T7554	parataxis	4,induced
R4378	T7571	T7570	punct	],4
R4379	T7572	T7542	punct	.,regulated
R4380	T7574	T7575	prep	In,induced
R4381	T7576	T7574	pobj	contrast,In
R4382	T7577	T7575	punct	", ",induced
R4383	T7578	T7579	compound	Snail,expression
R4384	T7579	T7575	nsubjpass	expression,induced
R4385	T7580	T7575	auxpass	was,induced
R4386	T7581	T7575	neg	not,induced
R4387	T7582	T7575	agent	by,induced
R4388	T7583	T7584	det	these,conditions
R4389	T7584	T7582	pobj	conditions,by
R4390	T7585	T7586	punct	(,5A
R4391	T7586	T7575	parataxis	5A,induced
R4392	T7587	T7586	compound	Figure,5A
R4393	T7588	T7586	punct	),5A
R4394	T7589	T7575	punct	.,induced
R4395	T7591	T7592	advmod	Thus,support
R4396	T7593	T7592	punct	", ",support
R4397	T7594	T7592	prep	despite,support
R4398	T7595	T7596	amod	essential,roles
R4399	T7596	T7594	pobj	roles,despite
R4400	T7597	T7596	prep	for,roles
R4401	T7598	T7597	pobj	Wnts,for
R4402	T7599	T7598	cc	and,Wnts
R4403	T7600	T7598	conj	noggin,Wnts
R4404	T7601	T7596	prep	in,roles
R4405	T7602	T7603	compound	hair,follicle
R4406	T7603	T7604	compound	follicle,specification
R4407	T7604	T7601	pobj	specification,in
R4408	T7605	T7606	punct	[,30
R4409	T7606	T7596	parataxis	30,roles
R4410	T7607	T7606	nummod	4,30
R4411	T7608	T7606	punct	",",30
R4412	T7609	T7606	nummod	29,30
R4413	T7610	T7606	punct	",",30
R4414	T7611	T7606	punct	],30
R4415	T7612	T7592	punct	", ",support
R4416	T7613	T7614	poss	our,studies
R4417	T7614	T7592	nsubj	studies,support
R4418	T7615	T7592	aux	did,support
R4419	T7616	T7592	neg	not,support
R4420	T7617	T7618	det	an,role
R4421	T7618	T7592	dobj	role,support
R4422	T7619	T7618	amod	essential,role
R4423	T7620	T7618	prep	for,role
R4424	T7621	T7622	det	these,signals
R4425	T7622	T7620	pobj	signals,for
R4426	T7623	T7618	prep	in,role
R4427	T7624	T7623	pcomp	governing,in
R4428	T7625	T7626	compound	Snail,expression
R4429	T7626	T7624	dobj	expression,governing
R4430	T7627	T7624	prep	in,governing
R4431	T7628	T7627	pobj	keratinocytes,in
R4432	T7629	T7592	punct	.,support
R4433	T7631	T7632	compound	TGF,β1
R4434	T7632	T7634	nsubjpass	β1,shown
R4435	T7633	T7632	punct	-,β1
R4436	T7635	T7634	aux	has,shown
R4437	T7636	T7634	auxpass	been,shown
R4438	T7637	T7638	aux	to,induce
R4439	T7638	T7634	xcomp	induce,shown
R4440	T7639	T7640	compound	Snail,members
R4441	T7640	T7638	dobj	members,induce
R4442	T7641	T7640	compound	family,members
R4443	T7642	T7638	prep	in,induce
R4444	T7643	T7642	pobj	hepatocytes,in
R4445	T7644	T7643	cc	and,hepatocytes
R4446	T7645	T7643	conj	heart,hepatocytes
R4447	T7646	T7647	punct	[,31
R4448	T7647	T7634	parataxis	31,shown
R4449	T7648	T7647	nummod	15,31
R4450	T7649	T7647	punct	", ",31
R4451	T7650	T7647	punct	],31
R4452	T7651	T7634	punct	.,shown
R4453	T7653	T7654	prep	In,inhibits
R4454	T7655	T7653	pobj	keratinocytes,In
R4455	T7656	T7654	punct	", ",inhibits
R4456	T7657	T7654	advmod	however,inhibits
R4457	T7658	T7654	punct	", ",inhibits
R4458	T7659	T7660	compound	TGF,β1
R4459	T7660	T7654	nsubj	β1,inhibits
R4460	T7661	T7660	punct	-,β1
R4461	T7662	T7663	compound	keratinocyte,growth
R4462	T7663	T7654	dobj	growth,inhibits
R4463	T7664	T7654	cc	and,inhibits
R4464	T7665	T7654	conj	seems,inhibits
R4465	T7666	T7667	aux	to,involved
R4466	T7667	T7665	xcomp	involved,seems
R4467	T7668	T7667	auxpass	be,involved
R4468	T7669	T7667	prep	in,involved
R4469	T7670	T7669	pcomp	triggering,in
R4470	T7671	T7672	det	the,phase
R4471	T7672	T7670	dobj	phase,triggering
R4472	T7673	T7672	amod	destructive,phase
R4473	T7674	T7672	prep	of,phase
R4474	T7675	T7676	det	the,follicle
R4475	T7676	T7674	pobj	follicle,of
R4476	T7677	T7676	amod	cycling,follicle
R4477	T7678	T7676	compound	hair,follicle
R4478	T7679	T7680	punct	[,32
R4479	T7680	T7665	parataxis	32,seems
R4480	T7681	T7680	punct	],32
R4481	T7682	T7654	punct	.,inhibits
R4482	T7684	T7685	prep	Of,blocked
R4483	T7686	T7687	det	the,mutations
R4484	T7687	T7684	pobj	mutations,Of
R4485	T7688	T7687	nmod	loss,mutations
R4486	T7689	T7688	punct	-,loss
R4487	T7690	T7688	prep	of,loss
R4488	T7691	T7690	punct	-,of
R4489	T7692	T7690	pobj	function,of
R4490	T7693	T7687	acl	generated,mutations
R4491	T7694	T7693	prep	in,generated
R4492	T7695	T7694	pobj	each,in
R4493	T7696	T7695	prep	of,each
R4494	T7697	T7698	det	the,genes
R4495	T7698	T7696	pobj	genes,of
R4496	T7699	T7700	compound	TGF,β
R4497	T7700	T7698	compound	β,genes
R4498	T7701	T7700	punct	-,β
R4499	T7702	T7685	punct	", ",blocked
R4500	T7703	T7704	advmod	only,state
R4501	T7704	T7685	nsubj	state,blocked
R4502	T7705	T7704	det	the,state
R4503	T7706	T7707	nmod	TGF,β2
R4504	T7707	T7704	nmod	β2,state
R4505	T7708	T7707	punct	-,β2
R4506	T7709	T7704	amod	null,state
R4507	T7710	T7711	compound	follicle,development
R4508	T7711	T7685	dobj	development,blocked
R4509	T7712	T7685	prep	at,blocked
R4510	T7713	T7714	det	the,stage
R4511	T7714	T7712	pobj	stage,at
R4512	T7715	T7716	compound	hair,bud
R4513	T7716	T7714	compound	bud,stage
R4514	T7717	T7718	punct	[,32
R4515	T7718	T7685	parataxis	32,blocked
R4516	T7719	T7718	punct	],32
R4517	T7720	T7685	punct	.,blocked
R4518	T7722	T7723	advmod	Thus,turned
R4519	T7724	T7723	punct	", ",turned
R4520	T7725	T7723	nsubj	we,turned
R4521	T7726	T7723	prep	towards,turned
R4522	T7727	T7726	pcomp	addressing,towards
R4523	T7728	T7729	mark	whether,involved
R4524	T7729	T7727	ccomp	involved,addressing
R4525	T7730	T7731	compound	TGF,β2
R4526	T7731	T7729	nsubjpass	β2,involved
R4527	T7732	T7731	punct	-,β2
R4528	T7733	T7729	aux	might,involved
R4529	T7734	T7729	auxpass	be,involved
R4530	T7735	T7729	prep	in,involved
R4531	T7736	T7735	pcomp	regulating,in
R4532	T7737	T7738	compound	Snail,expression
R4533	T7738	T7736	dobj	expression,regulating
R4534	T7739	T7736	prep	in,regulating
R4535	T7740	T7739	pobj	keratinocytes,in
R4536	T7741	T7740	acl	isolated,keratinocytes
R4537	T7742	T7741	prep	from,isolated
R4538	T7743	T7744	det	the,layer
R4539	T7744	T7742	pobj	layer,from
R4540	T7745	T7744	amod	basal,layer
R4541	T7746	T7744	prep	of,layer
R4542	T7747	T7748	det	the,epidermis
R4543	T7748	T7746	pobj	epidermis,of
R4544	T7749	T7723	punct	.,turned
R4545	T7751	T7752	mark	Though,is
R4546	T7752	T7754	advcl	is,are
R4547	T7753	T7752	expl	there,is
R4548	T7755	T7756	det	no,system
R4549	T7756	T7752	attr	system,is
R4550	T7757	T7758	compound	cell,culture
R4551	T7758	T7756	compound	culture,system
R4552	T7759	T7756	amod	available,system
R4553	T7760	T7761	aux	to,study
R4554	T7761	T7759	xcomp	study,available
R4555	T7762	T7761	advmod	specifically,study
R4556	T7763	T7764	amod	placodal,cells
R4557	T7764	T7761	dobj	cells,study
R4558	T7765	T7754	punct	", ",are
R4559	T7766	T7767	det	these,keratinocytes
R4560	T7767	T7754	nsubj	keratinocytes,are
R4561	T7768	T7769	poss	their,progenitors
R4562	T7769	T7754	attr	progenitors,are
R4563	T7770	T7754	cc	and,are
R4564	T7771	T7754	conj	are,are
R4565	T7772	T7773	det	the,approximation
R4566	T7773	T7771	attr	approximation,are
R4567	T7774	T7773	amod	closest,approximation
R4568	T7775	T7773	amod	available,approximation
R4569	T7776	T7777	aux	to,study
R4570	T7777	T7775	xcomp	study,available
R4571	T7778	T7779	det	the,behavior
R4572	T7779	T7777	dobj	behavior,study
R4573	T7780	T7779	prep	of,behavior
R4574	T7781	T7782	amod	epithelial,cells
R4575	T7782	T7780	pobj	cells,of
R4576	T7783	T7782	prep	of,cells
R4577	T7784	T7785	det	the,placode
R4578	T7785	T7783	pobj	placode,of
R4579	T7786	T7754	punct	.,are
R4580	T7788	T7789	advmod	Interestingly,caused
R4581	T7790	T7789	punct	", ",caused
R4582	T7791	T7789	nsubj	treatment,caused
R4583	T7792	T7791	prep	of,treatment
R4584	T7793	T7794	amod	cultured,keratinocytes
R4585	T7794	T7792	pobj	keratinocytes,of
R4586	T7795	T7791	prep	with,treatment
R4587	T7796	T7797	advmod	as,5
R4588	T7797	T7800	nummod	5,ng
R4589	T7798	T7797	amod	little,5
R4590	T7799	T7797	quantmod	as,5
R4591	T7800	T7795	pobj	ng,with
R4592	T7801	T7802	punct	/,ml
R4593	T7802	T7800	prep	ml,ng
R4594	T7803	T7800	prep	of,ng
R4595	T7804	T7805	compound	TGF,β2
R4596	T7805	T7803	pobj	β2,of
R4597	T7806	T7805	punct	-,β2
R4598	T7807	T7808	det	a,induction
R4599	T7808	T7789	dobj	induction,caused
R4600	T7809	T7808	amod	rapid,induction
R4601	T7810	T7809	cc	and,rapid
R4602	T7811	T7809	conj	transient,rapid
R4603	T7812	T7808	prep	of,induction
R4604	T7813	T7812	pobj	Snail,of
R4605	T7814	T7815	punct	(,5B
R4606	T7815	T7789	parataxis	5B,caused
R4607	T7816	T7815	compound	Figure,5B
R4608	T7817	T7815	punct	),5B
R4609	T7818	T7789	punct	.,caused
R4610	T7820	T7821	prep	Following,detected
R4611	T7822	T7823	det	this,treatment
R4612	T7823	T7820	pobj	treatment,Following
R4613	T7824	T7821	punct	", ",detected
R4614	T7825	T7826	compound	Snail,protein
R4615	T7826	T7821	nsubjpass	protein,detected
R4616	T7827	T7821	auxpass	was,detected
R4617	T7828	T7821	prep	within,detected
R4618	T7829	T7830	nummod	30,min
R4619	T7830	T7828	pobj	min,within
R4620	T7831	T7821	punct	", ",detected
R4621	T7832	T7821	conj	peaked,detected
R4622	T7833	T7832	prep	at,peaked
R4623	T7834	T7835	nummod	2,h
R4624	T7835	T7833	pobj	h,at
R4625	T7836	T7832	punct	", ",peaked
R4626	T7837	T7832	cc	and,peaked
R4627	T7838	T7839	advmod	then,declined
R4628	T7839	T7832	conj	declined,peaked
R4629	T7840	T7839	advmod	thereafter,declined
R4630	T7841	T7821	punct	.,detected
R4631	T7843	T7844	det	The,induction
R4632	T7844	T7845	nsubj	induction,appeared
R4633	T7846	T7844	prep	of,induction
R4634	T7847	T7846	pobj	Snail,of
R4635	T7848	T7849	aux	to,be
R4636	T7849	T7845	xcomp	be,appeared
R4637	T7850	T7849	acomp	specific,be
R4638	T7851	T7850	prep	for,specific
R4639	T7852	T7853	det	the,form
R4640	T7853	T7851	pobj	form,for
R4641	T7854	T7853	amod	active,form
R4642	T7855	T7853	prep	of,form
R4643	T7856	T7857	det	the,factor
R4644	T7857	T7855	pobj	factor,of
R4645	T7858	T7857	compound	growth,factor
R4646	T7859	T7849	punct	", ",be
R4647	T7860	T7861	mark	as,obliterated
R4648	T7861	T7849	advcl	obliterated,be
R4649	T7862	T7861	nsubj	pretreatment,obliterated
R4650	T7863	T7862	prep	of,pretreatment
R4651	T7864	T7865	compound	TGF,β2
R4652	T7865	T7863	pobj	β2,of
R4653	T7866	T7865	punct	-,β2
R4654	T7867	T7862	prep	for,pretreatment
R4655	T7868	T7869	nummod	10,min
R4656	T7869	T7867	pobj	min,for
R4657	T7870	T7862	prep	at,pretreatment
R4658	T7871	T7872	nummod	100,°C
R4659	T7872	T7870	pobj	°C,at
R4660	T7873	T7874	det	the,response
R4661	T7874	T7861	dobj	response,obliterated
R4662	T7875	T7876	punct	[,lanes
R4663	T7876	T7849	parataxis	lanes,be
R4664	T7877	T7878	compound	Figure,5B
R4665	T7878	T7876	dep	5B,lanes
R4666	T7879	T7876	punct	", ",lanes
R4667	T7880	T7876	acl	marked,lanes
R4668	T7881	T7880	punct	(,marked
R4669	T7882	T7880	oprd	–,marked
R4670	T7883	T7876	punct	),lanes
R4671	T7884	T7876	punct	],lanes
R4672	T7885	T7845	punct	.,appeared
R4673	T7887	T7888	prep	By,maintained
R4674	T7889	T7887	pobj	contrast,By
R4675	T7890	T7888	punct	", ",maintained
R4676	T7891	T7892	mark	although,remained
R4677	T7892	T7888	advcl	remained,maintained
R4678	T7893	T7894	compound	TGF,β
R4679	T7894	T7896	compound	β,receptor
R4680	T7895	T7894	punct	-,β
R4681	T7896	T7897	compound	receptor,activation
R4682	T7897	T7892	nsubj	activation,remained
R4683	T7898	T7892	acomp	elevated,remained
R4684	T7899	T7892	prep	during,remained
R4685	T7900	T7901	det	the,duration
R4686	T7901	T7899	pobj	duration,during
R4687	T7902	T7901	prep	of,duration
R4688	T7903	T7904	det	the,experiment
R4689	T7904	T7902	pobj	experiment,of
R4690	T7905	T7892	punct	(,remained
R4691	T7906	T7907	mark	as,measured
R4692	T7907	T7892	advcl	measured,remained
R4693	T7908	T7907	prep	by,measured
R4694	T7909	T7910	det	the,phosphorylation
R4695	T7910	T7908	pobj	phosphorylation,by
R4696	T7911	T7910	amod	sustained,phosphorylation
R4697	T7912	T7910	prep	of,phosphorylation
R4698	T7913	T7914	det	the,effector
R4699	T7914	T7912	pobj	effector,of
R4700	T7915	T7914	amod	downstream,effector
R4701	T7916	T7914	appos	SMAD2,effector
R4702	T7917	T7888	punct	),maintained
R4703	T7918	T7919	compound	Snail,expression
R4704	T7919	T7888	nsubjpass	expression,maintained
R4705	T7920	T7888	aux	could,maintained
R4706	T7921	T7888	neg	not,maintained
R4707	T7922	T7888	auxpass	be,maintained
R4708	T7923	T7924	punct	(,5B
R4709	T7924	T7888	parataxis	5B,maintained
R4710	T7925	T7924	compound	Figure,5B
R4711	T7926	T7924	punct	),5B
R4712	T7927	T7888	punct	.,maintained
R4713	T7929	T7930	advmod	Thus,seemed
R4714	T7931	T7930	punct	", ",seemed
R4715	T7932	T7933	mark	although,correlated
R4716	T7933	T7930	advcl	correlated,seemed
R4717	T7934	T7935	compound	Snail,expression
R4718	T7935	T7933	nsubj	expression,correlated
R4719	T7936	T7933	prep	with,correlated
R4720	T7937	T7938	amod	phosphorylated,SMAD2
R4721	T7938	T7939	nmod	SMAD2,induction
R4722	T7939	T7936	pobj	induction,with
R4723	T7940	T7938	punct	(,SMAD2
R4724	T7941	T7938	appos	pSMAD2,SMAD2
R4725	T7942	T7939	punct	),induction
R4726	T7943	T7930	punct	", ",seemed
R4727	T7944	T7945	poss	its,decline
R4728	T7945	T7930	nsubj	decline,seemed
R4729	T7946	T7947	aux	to,rely
R4730	T7947	T7930	xcomp	rely,seemed
R4731	T7948	T7947	prep	on,rely
R4732	T7949	T7950	amod	secondary,events
R4733	T7950	T7948	pobj	events,on
R4734	T7951	T7950	amod	downstream,events
R4735	T7952	T7930	punct	.,seemed
R4736	T7954	T7955	det	The,kinetics
R4737	T7955	T7957	nsubjpass	kinetics,reflected
R4738	T7956	T7955	amod	rapid,kinetics
R4739	T7958	T7955	prep	of,kinetics
R4740	T7959	T7960	compound	Snail,expression
R4741	T7960	T7958	pobj	expression,of
R4742	T7961	T7957	auxpass	were,reflected
R4743	T7962	T7957	prep	at,reflected
R4744	T7963	T7964	det	the,level
R4745	T7964	T7962	pobj	level,at
R4746	T7965	T7964	compound	mRNA,level
R4747	T7966	T7957	punct	", ",reflected
R4748	T7967	T7957	advcl	suggesting,reflected
R4749	T7968	T7969	mark	that,be
R4750	T7969	T7967	ccomp	be,suggesting
R4751	T7970	T7971	compound	Snail,promoter
R4752	T7971	T7972	compound	promoter,activity
R4753	T7972	T7969	nsubj	activity,be
R4754	T7973	T7972	prep	in,activity
R4755	T7974	T7973	pobj	keratinocytes,in
R4756	T7975	T7969	aux	might,be
R4757	T7976	T7969	acomp	sensitive,be
R4758	T7977	T7976	prep	to,sensitive
R4759	T7978	T7979	compound	TGF,β2
R4760	T7979	T7981	compound	β2,signaling
R4761	T7980	T7979	punct	-,β2
R4762	T7981	T7977	pobj	signaling,to
R4763	T7982	T7983	punct	(,5C
R4764	T7983	T7957	parataxis	5C,reflected
R4765	T7984	T7983	compound	Figure,5C
R4766	T7985	T7983	punct	),5C
R4767	T7986	T7957	punct	.,reflected
R4768	T7988	T7989	aux	To,test
R4769	T7989	T7990	advcl	test,engineered
R4770	T7991	T7992	det	this,possibility
R4771	T7992	T7989	dobj	possibility,test
R4772	T7993	T7990	punct	", ",engineered
R4773	T7994	T7990	nsubj	we,engineered
R4774	T7995	T7996	det	a,transgene
R4775	T7996	T7990	dobj	transgene,engineered
R4776	T7997	T7996	acl	driving,transgene
R4777	T7998	T7999	det	the,reporter
R4778	T7999	T7997	dobj	reporter,driving
R4779	T8000	T8001	compound	β,galactosidase
R4780	T8001	T7999	compound	galactosidase,reporter
R4781	T8002	T8001	punct	-,galactosidase
R4782	T8003	T7997	prep	under,driving
R4783	T8004	T8005	det	the,control
R4784	T8005	T8003	pobj	control,under
R4785	T8006	T8005	prep	of,control
R4786	T8007	T8008	advmod	approximately,2.2
R4787	T8008	T8009	nummod	2.2,kb
R4788	T8009	T8006	pobj	kb,of
R4789	T8010	T8009	prep	of,kb
R4790	T8011	T8012	compound	promoter,sequence
R4791	T8012	T8010	pobj	sequence,of
R4792	T8013	T8012	acl	located,sequence
R4793	T8014	T8013	npadvmod	5,located
R4794	T8015	T8014	punct	′,5
R4795	T8016	T8014	prep	from,5
R4796	T8017	T8018	det	the,site
R4797	T8018	T8016	pobj	site,from
R4798	T8019	T8018	compound	transcription,site
R4799	T8020	T8018	compound	initiation,site
R4800	T8021	T8018	prep	of,site
R4801	T8022	T8023	det	the,gene
R4802	T8023	T8021	pobj	gene,of
R4803	T8024	T8023	compound	mouse,gene
R4804	T8025	T8023	compound	Snail,gene
R4805	T8026	T7990	punct	.,engineered
R4806	T8028	T8029	prep	At,treated
R4807	T8030	T8031	nummod	2,d
R4808	T8031	T8028	pobj	d,At
R4809	T8032	T8031	prep	after,d
R4810	T8033	T8034	amod	transient,transfection
R4811	T8034	T8032	pobj	transfection,after
R4812	T8035	T8029	punct	", ",treated
R4813	T8036	T8029	nsubjpass	keratinocytes,treated
R4814	T8037	T8029	auxpass	were,treated
R4815	T8038	T8029	prep	with,treated
R4816	T8039	T8040	compound	TGF,β2
R4817	T8040	T8038	pobj	β2,with
R4818	T8041	T8040	punct	-,β2
R4819	T8042	T8043	punct	(,0
R4820	T8043	T8029	parataxis	0,treated
R4821	T8044	T8043	nsubj	t,0
R4822	T8045	T8043	punct	=,0
R4823	T8046	T8043	punct	),0
R4824	T8047	T8029	cc	and,treated
R4825	T8048	T8049	advmod	then,assayed
R4826	T8049	T8029	conj	assayed,treated
R4827	T8050	T8049	prep	for,assayed
R4828	T8051	T8052	compound	transgene,activity
R4829	T8052	T8050	pobj	activity,for
R4830	T8053	T8049	prep	over,assayed
R4831	T8054	T8055	det	the,course
R4832	T8055	T8053	pobj	course,over
R4833	T8056	T8055	amod	same,course
R4834	T8057	T8055	compound	time,course
R4835	T8058	T8059	prep	in,observed
R4836	T8059	T8055	relcl	observed,course
R4837	T8060	T8058	pobj	which,in
R4838	T8061	T8059	nsubj	we,observed
R4839	T8062	T8059	aux	had,observed
R4840	T8063	T8064	compound	Snail,protein
R4841	T8064	T8065	compound	protein,induction
R4842	T8065	T8059	dobj	induction,observed
R4843	T8066	T8029	punct	.,treated
R4844	T8068	T8069	det	The,results
R4845	T8069	T8070	nsubjpass	results,presented
R4846	T8071	T8069	prep	of,results
R4847	T8072	T8073	det	this,experiment
R4848	T8073	T8071	pobj	experiment,of
R4849	T8074	T8070	auxpass	are,presented
R4850	T8075	T8070	prep	in,presented
R4851	T8076	T8077	compound	Figure,5D
R4852	T8077	T8075	pobj	5D,in
R4853	T8078	T8070	punct	.,presented
R4854	T8080	T8081	prep	Within,increased
R4855	T8082	T8083	nummod	0.5,h
R4856	T8083	T8080	pobj	h,Within
R4857	T8084	T8083	prep	of,h
R4858	T8085	T8086	compound	TGF,β2
R4859	T8086	T8088	compound	β2,treatment
R4860	T8087	T8086	punct	-,β2
R4861	T8088	T8084	pobj	treatment,of
R4862	T8089	T8081	punct	", ",increased
R4863	T8090	T8091	compound	Snail,promoter
R4864	T8091	T8092	compound	promoter,activity
R4865	T8092	T8081	nsubj	activity,increased
R4866	T8093	T8081	aux	had,increased
R4867	T8094	T8081	advmod	3-fold,increased
R4868	T8095	T8081	punct	", ",increased
R4869	T8096	T8081	cc	and,increased
R4870	T8097	T8098	prep	by,peaked
R4871	T8098	T8081	conj	peaked,increased
R4872	T8099	T8100	nummod	2,h
R4873	T8100	T8097	pobj	h,by
R4874	T8101	T8098	punct	", ",peaked
R4875	T8102	T8098	nsubj	it,peaked
R4876	T8103	T8098	prep	to,peaked
R4877	T8104	T8105	advmod	approximately,10-fold
R4878	T8105	T8103	pcomp	10-fold,to
R4879	T8106	T8105	prep	over,10-fold
R4880	T8107	T8108	compound	control,levels
R4881	T8108	T8106	pobj	levels,over
R4882	T8109	T8110	punct	(,5D
R4883	T8110	T8098	parataxis	5D,peaked
R4884	T8111	T8110	compound	Figure,5D
R4885	T8112	T8110	punct	),5D
R4886	T8113	T8098	punct	.,peaked
R4887	T8115	T8116	advmod	Thereafter,returned
R4888	T8117	T8116	punct	", ",returned
R4889	T8118	T8119	compound	Snail,promoter
R4890	T8119	T8120	compound	promoter,activity
R4891	T8120	T8116	nsubj	activity,returned
R4892	T8121	T8116	advmod	rapidly,returned
R4893	T8122	T8116	prep	to,returned
R4894	T8123	T8124	det	the,levels
R4895	T8124	T8122	pobj	levels,to
R4896	T8125	T8124	amod	basal,levels
R4897	T8126	T8124	acl	seen,levels
R4898	T8127	T8126	prep	in,seen
R4899	T8128	T8129	amod	unstimulated,keratinocytes
R4900	T8129	T8127	pobj	keratinocytes,in
R4901	T8130	T8116	punct	.,returned
R4902	T8132	T8133	det	The,kinetics
R4903	T8133	T8134	nsubj	kinetics,paralleled
R4904	T8135	T8133	prep	of,kinetics
R4905	T8136	T8137	compound	Snail,promoter
R4906	T8137	T8138	compound	promoter,activity
R4907	T8138	T8135	pobj	activity,of
R4908	T8139	T8134	advmod	closely,paralleled
R4909	T8140	T8134	dobj	those,paralleled
R4910	T8141	T8140	acl	observed,those
R4911	T8142	T8141	prep	for,observed
R4912	T8143	T8144	compound	Snail,protein
R4913	T8144	T8145	compound	protein,induction
R4914	T8145	T8142	pobj	induction,for
R4915	T8146	T8134	punct	.,paralleled
R4916	T8148	T8149	advmod	Moreover,appeared
R4917	T8150	T8149	punct	", ",appeared
R4918	T8151	T8152	det	the,effects
R4919	T8152	T8149	nsubj	effects,appeared
R4920	T8153	T8152	amod	stimulatory,effects
R4921	T8154	T8155	aux	to,be
R4922	T8155	T8149	xcomp	be,appeared
R4923	T8156	T8155	acomp	specific,be
R4924	T8157	T8156	prep	to,specific
R4925	T8158	T8159	compound	TGF,β2
R4926	T8159	T8157	pobj	β2,to
R4927	T8160	T8159	punct	-,β2
R4928	T8161	T8155	punct	", ",be
R4929	T8162	T8163	mark	since,abrogated
R4930	T8163	T8155	advcl	abrogated,be
R4931	T8164	T8163	nsubjpass	they,abrogated
R4932	T8165	T8163	auxpass	were,abrogated
R4933	T8166	T8167	preconj	either,by
R4934	T8167	T8163	prep	by,abrogated
R4935	T8168	T8169	compound	heat,inactivation
R4936	T8169	T8167	pobj	inactivation,by
R4937	T8170	T8169	prep	of,inactivation
R4938	T8171	T8172	det	the,protein
R4939	T8172	T8170	pobj	protein,of
R4940	T8173	T8174	compound	TGF,β2
R4941	T8174	T8172	compound	β2,protein
R4942	T8175	T8174	punct	-,β2
R4943	T8176	T8167	cc	or,by
R4944	T8177	T8167	conj	by,by
R4945	T8178	T8177	pobj	mutation,by
R4946	T8179	T8178	prep	of,mutation
R4947	T8180	T8181	det	a,element
R4948	T8181	T8179	pobj	element,of
R4949	T8182	T8181	amod	putative,element
R4950	T8183	T8184	compound	SMAD,binding
R4951	T8184	T8181	compound	binding,element
R4952	T8185	T8181	acl	located,element
R4953	T8186	T8187	quantmod	about,1.8
R4954	T8187	T8188	nummod	1.8,kb
R4955	T8188	T8185	npadvmod	kb,located
R4956	T8189	T8188	nummod	5,kb
R4957	T8190	T8188	punct	′,kb
R4958	T8191	T8188	prep	from,kb
R4959	T8192	T8193	det	the,site
R4960	T8193	T8191	pobj	site,from
R4961	T8194	T8193	compound	Snail,site
R4962	T8195	T8193	compound	transcription,site
R4963	T8196	T8193	compound	start,site
R4964	T8197	T8198	punct	(,5D
R4965	T8198	T8163	parataxis	5D,abrogated
R4966	T8199	T8198	compound	Figure,5D
R4967	T8200	T8198	punct	),5D
R4968	T8201	T8149	punct	.,appeared
R4969	T8203	T8204	advcl	Taken,suggested
R4970	T8205	T8203	advmod	together,Taken
R4971	T8206	T8204	punct	", ",suggested
R4972	T8207	T8208	det	these,results
R4973	T8208	T8204	nsubj	results,suggested
R4974	T8209	T8210	mark	that,results
R4975	T8210	T8204	advcl	results,suggested
R4976	T8211	T8210	prep	in,results
R4977	T8212	T8211	pobj	keratinocytes,in
R4978	T8213	T8210	punct	", ",results
R4979	T8214	T8215	compound	TGF,β2
R4980	T8215	T8217	compound	β2,signaling
R4981	T8216	T8215	punct	-,β2
R4982	T8217	T8210	nsubj	signaling,results
R4983	T8218	T8210	prep	in,results
R4984	T8219	T8220	det	a,activation
R4985	T8220	T8218	pobj	activation,in
R4986	T8221	T8222	npadvmod	pSMAD2,dependent
R4987	T8222	T8220	amod	dependent,activation
R4988	T8223	T8222	punct	-,dependent
R4989	T8224	T8220	amod	transient,activation
R4990	T8225	T8220	prep	of,activation
R4991	T8226	T8227	det	the,gene
R4992	T8227	T8225	pobj	gene,of
R4993	T8228	T8227	compound	Snail,gene
R4994	T8229	T8210	punct	", ",results
R4995	T8230	T8210	cc	and,results
R4996	T8231	T8232	mark	that,relies
R4997	T8232	T8210	conj	relies,results
R4998	T8233	T8232	nsubj	maintenance,relies
R4999	T8234	T8233	prep	of,maintenance
R5000	T8235	T8236	compound	Snail,protein
R5001	T8236	T8234	pobj	protein,of
R5002	T8237	T8232	punct	", ",relies
R5003	T8238	T8232	prep	in,relies
R5004	T8239	T8238	pobj	part,in
R5005	T8240	T8232	punct	", ",relies
R5006	T8241	T8232	prep	upon,relies
R5007	T8242	T8243	amod	sustained,activity
R5008	T8243	T8241	pobj	activity,upon
R5009	T8244	T8243	compound	promoter,activity
R5010	T8245	T8204	punct	.,suggested
R5011	T8247	T8248	det	The,brevity
R5012	T8248	T8249	nsubj	brevity,resembled
R5013	T8250	T8248	prep	of,brevity
R5014	T8251	T8252	nmod	Snail,gene
R5015	T8252	T8253	nmod	gene,induction
R5016	T8253	T8250	pobj	induction,of
R5017	T8254	T8252	cc	and,gene
R5018	T8255	T8252	conj	protein,gene
R5019	T8256	T8253	prep	in,induction
R5020	T8257	T8258	compound	TGF,β2
R5021	T8258	T8260	npadvmod	β2,treated
R5022	T8259	T8258	punct	-,β2
R5023	T8260	T8261	amod	treated,keratinocytes
R5024	T8261	T8256	pobj	keratinocytes,in
R5025	T8262	T8261	amod	cultured,keratinocytes
R5026	T8263	T8264	det	the,appearance
R5027	T8264	T8249	dobj	appearance,resembled
R5028	T8265	T8264	amod	temporal,appearance
R5029	T8266	T8264	prep	of,appearance
R5030	T8267	T8268	compound	Snail,mRNA
R5031	T8268	T8266	pobj	mRNA,of
R5032	T8269	T8268	cc	and,mRNA
R5033	T8270	T8268	conj	protein,mRNA
R5034	T8271	T8264	prep	at,appearance
R5035	T8272	T8273	det	the,initiation
R5036	T8273	T8271	pobj	initiation,at
R5037	T8274	T8273	prep	of,initiation
R5038	T8275	T8276	compound	hair,follicle
R5039	T8276	T8277	compound	follicle,morphogenesis
R5040	T8277	T8274	pobj	morphogenesis,of
R5041	T8278	T8249	prep	in,resembled
R5042	T8279	T8280	amod	embryonic,skin
R5043	T8280	T8278	pobj	skin,in
R5044	T8281	T8280	compound	mouse,skin
R5045	T8282	T8249	punct	.,resembled
R5046	T8284	T8285	aux	To,test
R5047	T8285	T8286	advcl	test,analyzed
R5048	T8287	T8288	mark	whether,required
R5049	T8288	T8285	ccomp	required,test
R5050	T8289	T8290	compound	TGF,β2
R5051	T8290	T8288	nsubjpass	β2,required
R5052	T8291	T8290	punct	-,β2
R5053	T8292	T8288	aux	might,required
R5054	T8293	T8288	auxpass	be,required
R5055	T8294	T8288	prep	for,required
R5056	T8295	T8296	compound	Snail,induction
R5057	T8296	T8294	pobj	induction,for
R5058	T8297	T8296	prep	in,induction
R5059	T8298	T8299	compound	hair,bud
R5060	T8299	T8300	compound	bud,formation
R5061	T8300	T8297	pobj	formation,in
R5062	T8301	T8302	advmod	in,vivo
R5063	T8302	T8288	advmod	vivo,required
R5064	T8303	T8286	punct	", ",analyzed
R5065	T8304	T8286	nsubj	we,analyzed
R5066	T8305	T8286	advmod	first,analyzed
R5067	T8306	T8307	mark	whether,expressed
R5068	T8307	T8286	ccomp	expressed,analyzed
R5069	T8308	T8309	compound	TGF,β2
R5070	T8309	T8307	nsubjpass	β2,expressed
R5071	T8310	T8309	punct	-,β2
R5072	T8311	T8307	auxpass	was,expressed
R5073	T8312	T8307	prep	in,expressed
R5074	T8313	T8312	cc	or,in
R5075	T8314	T8312	conj	around,in
R5076	T8315	T8316	det	the,bud
R5077	T8316	T8314	pobj	bud,around
R5078	T8317	T8316	compound	hair,bud
R5079	T8318	T8286	punct	.,analyzed
R5080	T8320	T8321	advcl	Consistent,labeled
R5081	T8322	T8320	prep	with,Consistent
R5082	T8323	T8324	amod	previous,observations
R5083	T8324	T8322	pobj	observations,with
R5084	T8325	T8326	punct	[,33
R5085	T8326	T8320	parataxis	33,Consistent
R5086	T8327	T8326	punct	],33
R5087	T8328	T8321	punct	", ",labeled
R5088	T8329	T8330	det	an,antibody
R5089	T8330	T8321	nsubj	antibody,labeled
R5090	T8331	T8332	amod	anti-TGF,β2
R5091	T8332	T8330	compound	β2,antibody
R5092	T8333	T8332	punct	-,β2
R5093	T8334	T8335	amod	developing,buds
R5094	T8335	T8321	dobj	buds,labeled
R5095	T8336	T8335	compound	hair,buds
R5096	T8337	T8338	punct	(,6A
R5097	T8338	T8321	parataxis	6A,labeled
R5098	T8339	T8338	compound	Figure,6A
R5099	T8340	T8338	punct	),6A
R5100	T8341	T8321	punct	.,labeled
R5101	T8343	T8344	det	This,labeling
R5102	T8344	T8345	nsubj	labeling,appeared
R5103	T8346	T8347	aux	to,be
R5104	T8347	T8345	xcomp	be,appeared
R5105	T8348	T8347	acomp	specific,be
R5106	T8349	T8350	mark	as,judged
R5107	T8350	T8347	advcl	judged,be
R5108	T8351	T8350	prep	by,judged
R5109	T8352	T8353	det	the,lack
R5110	T8353	T8351	pobj	lack,by
R5111	T8354	T8353	prep	of,lack
R5112	T8355	T8354	pobj	staining,of
R5113	T8356	T8353	prep	in,lack
R5114	T8357	T8358	compound	follicle,buds
R5115	T8358	T8356	pobj	buds,in
R5116	T8359	T8358	prep	from,buds
R5117	T8360	T8359	pobj	mice,from
R5118	T8361	T8360	amod	homozygous,mice
R5119	T8362	T8361	prep	for,homozygous
R5120	T8363	T8364	det	a,mutation
R5121	T8364	T8362	pobj	mutation,for
R5122	T8365	T8366	nmod	TGF,β2
R5123	T8366	T8364	nmod	β2,mutation
R5124	T8367	T8366	punct	-,β2
R5125	T8368	T8364	amod	null,mutation
R5126	T8369	T8370	punct	(,34
R5127	T8370	T8345	parataxis	34,appeared
R5128	T8371	T8372	compound	Figure,6A
R5129	T8372	T8370	dep	6A,34
R5130	T8373	T8370	punct	;,34
R5131	T8374	T8370	punct	[,34
R5132	T8375	T8370	punct	],34
R5133	T8376	T8370	punct	),34
R5134	T8377	T8345	punct	.,appeared
R5135	T8379	T8380	advmod	Moreover,expressed
R5136	T8381	T8380	punct	", ",expressed
R5137	T8382	T8383	det	the,effector
R5138	T8383	T8380	nsubjpass	effector,expressed
R5139	T8384	T8383	amod	downstream,effector
R5140	T8385	T8383	prep	of,effector
R5141	T8386	T8387	compound	TGF,β2
R5142	T8387	T8389	compound	β2,signaling
R5143	T8388	T8387	punct	-,β2
R5144	T8389	T8385	pobj	signaling,of
R5145	T8390	T8383	punct	", ",effector
R5146	T8391	T8383	appos	pSMAD2,effector
R5147	T8392	T8380	punct	", ",expressed
R5148	T8393	T8380	auxpass	was,expressed
R5149	T8394	T8380	advmod	also,expressed
R5150	T8395	T8380	prep	in,expressed
R5151	T8396	T8397	nmod	WT,buds
R5152	T8397	T8395	pobj	buds,in
R5153	T8398	T8396	punct	", ",WT
R5154	T8399	T8396	cc	but,WT
R5155	T8400	T8399	neg	not,but
R5156	T8401	T8402	compound	TGF,β2
R5157	T8402	T8404	npadvmod	β2,null
R5158	T8403	T8402	punct	-,β2
R5159	T8404	T8396	conj	null,WT
R5160	T8405	T8404	punct	-,null
R5161	T8406	T8397	punct	", ",buds
R5162	T8407	T8397	compound	hair,buds
R5163	T8408	T8409	punct	(,6B
R5164	T8409	T8380	parataxis	6B,expressed
R5165	T8410	T8409	compound	Figure,6B
R5166	T8411	T8409	punct	),6B
R5167	T8412	T8380	punct	.,expressed
R5168	T8414	T8415	advmod	Together,underscore
R5169	T8416	T8415	punct	", ",underscore
R5170	T8417	T8418	det	these,data
R5171	T8418	T8415	nsubj	data,underscore
R5172	T8419	T8420	det	the,importance
R5173	T8420	T8415	dobj	importance,underscore
R5174	T8421	T8420	prep	of,importance
R5175	T8422	T8423	det	the,isoform
R5176	T8423	T8421	pobj	isoform,of
R5177	T8424	T8425	compound	TGF,β2
R5178	T8425	T8423	compound	β2,isoform
R5179	T8426	T8425	punct	-,β2
R5180	T8427	T8415	prep	despite,underscore
R5181	T8428	T8427	pobj	expression,despite
R5182	T8429	T8428	prep	of,expression
R5183	T8430	T8431	preconj	both,β1
R5184	T8431	T8429	pobj	β1,of
R5185	T8432	T8431	compound	TGF,β1
R5186	T8433	T8431	punct	-,β1
R5187	T8434	T8431	cc	and,β1
R5188	T8435	T8436	compound	TGF,β2
R5189	T8436	T8431	conj	β2,β1
R5190	T8437	T8436	punct	-,β2
R5191	T8438	T8428	prep	in,expression
R5192	T8439	T8440	amod	developing,buds
R5193	T8440	T8438	pobj	buds,in
R5194	T8441	T8440	compound	hair,buds
R5195	T8442	T8428	prep	at,expression
R5196	T8443	T8444	det	this,stage
R5197	T8444	T8442	pobj	stage,at
R5198	T8445	T8415	punct	.,underscore
R5199	T8447	T8448	aux	To,explore
R5200	T8448	T8450	advcl	explore,examined
R5201	T8449	T8448	advmod	further,explore
R5202	T8451	T8452	det	the,relation
R5203	T8452	T8448	dobj	relation,explore
R5204	T8453	T8452	amod	possible,relation
R5205	T8454	T8452	prep	between,relation
R5206	T8455	T8454	pobj	Snail,between
R5207	T8456	T8455	cc	and,Snail
R5208	T8457	T8458	compound	TGF,β2
R5209	T8458	T8455	conj	β2,Snail
R5210	T8459	T8458	punct	-,β2
R5211	T8460	T8450	punct	", ",examined
R5212	T8461	T8450	nsubj	we,examined
R5213	T8462	T8463	det	the,status
R5214	T8463	T8450	dobj	status,examined
R5215	T8464	T8463	prep	of,status
R5216	T8465	T8466	compound	Snail,expression
R5217	T8466	T8464	pobj	expression,of
R5218	T8467	T8463	prep	in,status
R5219	T8468	T8469	compound	TGF,β2
R5220	T8469	T8471	npadvmod	β2,null
R5221	T8470	T8469	punct	-,β2
R5222	T8471	T8473	amod	null,buds
R5223	T8472	T8471	punct	-,null
R5224	T8473	T8467	pobj	buds,in
R5225	T8474	T8473	compound	hair,buds
R5226	T8475	T8450	punct	.,examined
R5227	T8477	T8478	mark	As,judged
R5228	T8478	T8479	advcl	judged,was
R5229	T8480	T8478	prep	by,judged
R5230	T8481	T8480	pobj	immunohistochemistry,by
R5231	T8482	T8479	punct	", ",was
R5232	T8483	T8484	compound	Snail,protein
R5233	T8484	T8479	nsubj	protein,was
R5234	T8485	T8479	acomp	absent,was
R5235	T8486	T8485	prep	from,absent
R5236	T8487	T8488	compound	E17.5,skin
R5237	T8488	T8486	pobj	skin,from
R5238	T8489	T8488	prep	of,skin
R5239	T8490	T8491	compound	TGF,β2
R5240	T8491	T8493	npadvmod	β2,null
R5241	T8492	T8491	punct	-,β2
R5242	T8493	T8495	amod	null,embryos
R5243	T8494	T8493	punct	-,null
R5244	T8495	T8489	pobj	embryos,of
R5245	T8496	T8486	cc	but,from
R5246	T8497	T8496	neg	not,but
R5247	T8498	T8486	conj	from,from
R5248	T8499	T8498	pobj	that,from
R5249	T8500	T8499	prep	of,that
R5250	T8501	T8502	compound	control,littermates
R5251	T8502	T8500	pobj	littermates,of
R5252	T8503	T8504	punct	(,6C
R5253	T8504	T8479	parataxis	6C,was
R5254	T8505	T8504	compound	Figure,6C
R5255	T8506	T8504	punct	),6C
R5256	T8507	T8479	punct	.,was
R5257	T8509	T8510	det	This,effect
R5258	T8510	T8511	nsubj	effect,appeared
R5259	T8512	T8513	aux	to,exerted
R5260	T8513	T8511	xcomp	exerted,appeared
R5261	T8514	T8513	auxpass	be,exerted
R5262	T8515	T8513	prep	at,exerted
R5263	T8516	T8517	det	the,level
R5264	T8517	T8515	pobj	level,at
R5265	T8518	T8517	amod	transcriptional,level
R5266	T8519	T8511	punct	", ",appeared
R5267	T8520	T8521	mark	since,found
R5268	T8521	T8511	advcl	found,appeared
R5269	T8522	T8523	compound	Snail,mRNAs
R5270	T8523	T8521	nsubjpass	mRNAs,found
R5271	T8524	T8521	auxpass	were,found
R5272	T8525	T8521	advmod	also,found
R5273	T8526	T8521	neg	not,found
R5274	T8527	T8521	prep	in,found
R5275	T8528	T8529	compound	TGF,β2
R5276	T8529	T8531	npadvmod	β2,null
R5277	T8530	T8529	punct	-,β2
R5278	T8531	T8532	amod	null,buds
R5279	T8532	T8527	pobj	buds,in
R5280	T8533	T8532	compound	hair,buds
R5281	T8534	T8521	prep	under,found
R5282	T8535	T8534	pobj	conditions,under
R5283	T8536	T8537	prep	in,detected
R5284	T8537	T8535	relcl	detected,conditions
R5285	T8538	T8536	pobj	which,in
R5286	T8539	T8540	det	the,signal
R5287	T8559	T8557	prep	in,detected
R5288	T8540	T8537	nsubjpass	signal,detected
R5289	T8541	T8537	auxpass	was,detected
R5290	T8560	T8561	nummod	2,wk
R5291	T8561	T8563	npadvmod	wk,old
R5292	T8542	T8537	advmod	readily,detected
R5293	T8562	T8561	punct	-,wk
R5294	T8563	T8565	amod	old,mice
R5295	T8564	T8563	punct	-,old
R5296	T8543	T8537	prep	in,detected
R5297	T8565	T8559	pobj	mice,in
R5298	T8566	T8567	compound	K14,Smad2
R5299	T8567	T8565	compound	Smad2,mice
R5300	T8568	T8567	punct	-,Smad2
R5301	T8569	T8565	compound	Tg,mice
R5302	T8544	T8545	det	the,buds
R5303	T8570	T8565	punct	", ",mice
R5304	T8571	T8572	dep	which,display
R5305	T8572	T8565	relcl	display,mice
R5306	T8545	T8543	pobj	buds,in
R5307	T8573	T8574	amod	elevated,signaling
R5308	T8546	T8545	compound	hair,buds
R5309	T8574	T8572	dobj	signaling,display
R5310	T8575	T8576	compound	TGF,β
R5311	T8547	T8545	prep	of,buds
R5312	T8576	T8574	compound	β,signaling
R5313	T8577	T8576	punct	-,β
R5314	T8578	T8572	prep	in,display
R5315	T8548	T8549	compound	littermate,skin
R5316	T8549	T8547	pobj	skin,of
R5317	T8579	T8578	pobj	skin,in
R5318	T8580	T8581	punct	[,35
R5319	T8550	T8551	punct	(,6D
R5320	T8581	T8559	parataxis	35,in
R5321	T8582	T8581	punct	],35
R5322	T8583	T8557	punct	", ",detected
R5323	T8551	T8521	parataxis	6D,found
R5324	T8584	T8585	compound	Snail,protein
R5325	T8585	T8557	nsubjpass	protein,detected
R5326	T8552	T8551	compound	Figure,6D
R5327	T8586	T8557	auxpass	was,detected
R5328	T8587	T8557	advmod	readily,detected
R5329	T8553	T8551	punct	),6D
R5330	T8588	T8557	prep	by,detected
R5331	T8554	T8511	punct	.,appeared
R5332	T8589	T8590	compound	Western,blot
R5333	T8590	T8591	compound	blot,analyses
R5334	T8591	T8588	pobj	analyses,by
R5335	T8592	T8557	punct	", ",detected
R5336	T8593	T8594	advmod	where,found
R5337	T8556	T8557	advmod	Conversely,detected
R5338	T8594	T8557	advcl	found,detected
R5339	T8595	T8594	nsubjpass	it,found
R5340	T8596	T8594	auxpass	was,found
R5341	T8597	T8594	neg	not,found
R5342	T8558	T8557	punct	", ",detected
R5343	T8598	T8594	prep	in,found
R5344	T8599	T8600	amod	postnatal,skin
R5345	T8600	T8598	pobj	skin,in
R5346	T8601	T8602	punct	(,6E
R5347	T8664	T8660	advmod	still,formed
R5348	T8602	T8557	parataxis	6E,detected
R5349	T8603	T8602	compound	Figure,6E
R5350	T8604	T8602	punct	),6E
R5351	T8605	T8557	punct	.,detected
R5352	T8607	T8608	advcl	Taken,provide
R5353	T8609	T8607	advmod	together,Taken
R5354	T8610	T8608	punct	", ",provide
R5355	T8611	T8612	det	these,results
R5356	T8666	T8660	prep	in,formed
R5357	T8612	T8608	nsubj	results,provide
R5358	T8613	T8614	amod	compelling,evidence
R5359	T8614	T8608	dobj	evidence,provide
R5360	T8615	T8616	mark	that,is
R5361	T8616	T8614	acl	is,evidence
R5362	T8667	T8668	compound	TGF,β2
R5363	T8617	T8618	compound	TGF,β2
R5364	T8618	T8616	nsubj	β2,is
R5365	T8619	T8618	punct	-,β2
R5366	T8668	T8670	npadvmod	β2,null
R5367	T8620	T8621	advmod	functionally,important
R5368	T8621	T8616	acomp	important,is
R5369	T8669	T8668	punct	-,β2
R5370	T8622	T8621	prep	for,important
R5371	T8623	T8622	pcomp	inducing,for
R5372	T8624	T8625	compound	Snail,expression
R5373	T8670	T8671	amod	null,skin
R5374	T8625	T8623	dobj	expression,inducing
R5375	T8626	T8625	compound	gene,expression
R5376	T8627	T8623	prep	in,inducing
R5377	T8671	T8666	pobj	skin,in
R5378	T8628	T8629	det	a,manner
R5379	T8629	T8627	pobj	manner,in
R5380	T8672	T8665	punct	", ",reduced
R5381	T8630	T8631	npadvmod	pSMAD,dependent
R5382	T8631	T8629	amod	dependent,manner
R5383	T8632	T8631	punct	-,dependent
R5384	T8673	T8674	poss	their,number
R5385	T8633	T8623	prep	in,inducing
R5386	T8634	T8635	amod	developing,buds
R5387	T8635	T8633	pobj	buds,in
R5388	T8674	T8665	nsubjpass	number,reduced
R5389	T8636	T8635	compound	hair,buds
R5390	T8637	T8608	punct	.,provide
R5391	T8675	T8665	auxpass	was,reduced
R5392	T8639	T8640	mark	Whether,contributes
R5393	T8640	T8644	csubjpass	contributes,addressed
R5394	T8676	T8665	prep	by,reduced
R5395	T8641	T8642	compound	pMARK,activity
R5396	T8642	T8640	nsubj	activity,contributes
R5397	T8643	T8640	advmod	also,contributes
R5398	T8677	T8678	advmod	approximately,50
R5399	T8645	T8640	prep	to,contributes
R5400	T8646	T8647	compound	Snail,induction
R5401	T8647	T8645	pobj	induction,to
R5402	T8648	T8644	auxpass	was,addressed
R5403	T8649	T8644	neg	not,addressed
R5404	T8678	T8679	nummod	50,%
R5405	T8650	T8644	prep	in,addressed
R5406	T8651	T8652	det	the,study
R5407	T8652	T8650	pobj	study,in
R5408	T8679	T8676	pobj	%,by
R5409	T8653	T8652	amod	present,study
R5410	T8654	T8655	punct	[,15
R5411	T8655	T8644	parataxis	15,addressed
R5412	T8680	T8681	punct	[,32
R5413	T8656	T8655	punct	],15
R5414	T8657	T8644	punct	.,addressed
R5415	T8681	T8665	parataxis	32,reduced
R5416	T8659	T8660	mark	Although,formed
R5417	T8660	T8665	advcl	formed,reduced
R5418	T8661	T8662	det	some,buds
R5419	T8682	T8681	punct	],32
R5420	T8662	T8660	nsubj	buds,formed
R5421	T8663	T8662	compound	hair,buds
R5422	T8683	T8665	punct	.,reduced
R5423	T8685	T8686	advmod	Thus,appear
R5424	T8687	T8686	punct	", ",appear
R5425	T8770	T8772	compound	β2,signaling
R5426	T8688	T8689	mark	although,impacts
R5427	T8689	T8686	advcl	impacts,appear
R5428	T8771	T8770	punct	-,β2
R5429	T8772	T8766	nsubj	signaling,appeared
R5430	T8690	T8691	det	the,pathway
R5431	T8773	T8774	aux	to,be
R5432	T8774	T8766	xcomp	be,appeared
R5433	T8775	T8776	det	an,factor
R5434	T8691	T8689	nsubj	pathway,impacts
R5435	T8776	T8774	attr	factor,be
R5436	T8777	T8776	amod	important,factor
R5437	T8692	T8691	acl	mediated,pathway
R5438	T8778	T8776	prep	for,factor
R5439	T8779	T8780	det	the,proliferation
R5440	T8693	T8692	prep	by,mediated
R5441	T8780	T8778	pobj	proliferation,for
R5442	T8781	T8780	amod	early,proliferation
R5443	T8782	T8783	dep	that,occurs
R5444	T8694	T8695	compound	TGF,β2
R5445	T8783	T8780	relcl	occurs,proliferation
R5446	T8784	T8783	prep	in,occurs
R5447	T8695	T8697	compound	β2,signaling
R5448	T8785	T8786	det	the,buds
R5449	T8786	T8784	pobj	buds,in
R5450	T8787	T8786	amod	developing,buds
R5451	T8696	T8695	punct	-,β2
R5452	T8788	T8786	compound	hair,buds
R5453	T8789	T8774	punct	", ",be
R5454	T8790	T8791	mark	as,judged
R5455	T8791	T8774	advcl	judged,be
R5456	T8792	T8791	prep	by,judged
R5457	T8793	T8794	amod	anti-Ki67,immunofluorescence
R5458	T8697	T8693	pobj	signaling,by
R5459	T8794	T8792	pobj	immunofluorescence,by
R5460	T8795	T8793	cc	and,anti-Ki67
R5461	T8698	T8699	det	the,step
R5462	T8796	T8793	conj	anti-pMAPK,anti-Ki67
R5463	T8797	T8798	punct	(,6F
R5464	T8798	T8774	parataxis	6F,be
R5465	T8699	T8689	dobj	step,impacts
R5466	T8799	T8798	compound	Figure,6F
R5467	T8800	T8798	cc	and,6F
R5468	T8801	T8798	conj	6G,6F
R5469	T8700	T8699	amod	earliest,step
R5470	T8802	T8798	punct	),6F
R5471	T8803	T8766	punct	.,appeared
R5472	T8701	T8699	prep	of,step
R5473	T8805	T8806	mark	If,stimulates
R5474	T8806	T8810	advcl	stimulates,expected
R5475	T8702	T8703	amod	epithelial,invagination
R5476	T8807	T8808	compound	TGF,β2
R5477	T8808	T8806	nsubj	β2,stimulates
R5478	T8809	T8808	punct	-,β2
R5479	T8703	T8701	pobj	invagination,of
R5480	T8811	T8812	compound	Snail,expression
R5481	T8812	T8806	dobj	expression,stimulates
R5482	T8704	T8686	punct	", ",appear
R5483	T8813	T8806	prep	in,stimulates
R5484	T8814	T8815	amod	developing,buds
R5485	T8815	T8813	pobj	buds,in
R5486	T8705	T8686	nsubj	it,appear
R5487	T8816	T8810	punct	", ",expected
R5488	T8817	T8810	nsubjpass	loss,expected
R5489	T8818	T8817	prep	of,loss
R5490	T8706	T8686	aux	does,appear
R5491	T8819	T8820	det	this,morphogen
R5492	T8820	T8818	pobj	morphogen,of
R5493	T8707	T8686	neg	not,appear
R5494	T8821	T8810	aux	would,expected
R5495	T8822	T8810	auxpass	be,expected
R5496	T8823	T8824	aux	to,affect
R5497	T8708	T8709	aux	to,be
R5498	T8824	T8810	xcomp	affect,expected
R5499	T8825	T8826	det	the,expression
R5500	T8826	T8824	dobj	expression,affect
R5501	T8709	T8686	xcomp	be,appear
R5502	T8827	T8826	prep	of,expression
R5503	T8828	T8827	pobj	genes,of
R5504	T8829	T8830	dep	that,repressed
R5505	T8830	T8828	relcl	repressed,genes
R5506	T8831	T8830	auxpass	are,repressed
R5507	T8832	T8830	advmod	typically,repressed
R5508	T8710	T8709	acomp	essential,be
R5509	T8833	T8830	agent	by,repressed
R5510	T8834	T8833	pobj	Snail,by
R5511	T8835	T8810	punct	.,expected
R5512	T8711	T8710	prep	for,essential
R5513	T8712	T8713	compound	bud,morphogenesis
R5514	T8837	T8838	mark	Since,is
R5515	T8713	T8711	pobj	morphogenesis,for
R5516	T8838	T8847	advcl	is,investigated
R5517	T8839	T8840	det	a,target
R5518	T8714	T8686	punct	.,appear
R5519	T8840	T8838	nsubj	target,is
R5520	T8841	T8840	amod	major,target
R5521	T8842	T8840	prep	for,target
R5522	T8843	T8844	npadvmod	Snail,mediated
R5523	T8716	T8717	advcl	Consistent,took
R5524	T8844	T8846	amod	mediated,repression
R5525	T8845	T8844	punct	-,mediated
R5526	T8846	T8842	pobj	repression,for
R5527	T8718	T8716	prep	with,Consistent
R5528	T8848	T8849	det	the,gene
R5529	T8849	T8838	attr	gene,is
R5530	T8850	T8851	compound	E,cadherin
R5531	T8719	T8720	det	this,notion
R5532	T8720	T8718	pobj	notion,with
R5533	T8721	T8717	punct	", ",took
R5534	T8851	T8849	compound	cadherin,gene
R5535	T8722	T8723	compound	basement,membrane
R5536	T8723	T8724	compound	membrane,remodeling
R5537	T8852	T8851	punct	-,cadherin
R5538	T8853	T8854	punct	[,13
R5539	T8854	T8838	parataxis	13,is
R5540	T8724	T8717	nsubj	remodeling,took
R5541	T8855	T8854	nummod	12,13
R5542	T8856	T8854	punct	",",13
R5543	T8725	T8717	advmod	still,took
R5544	T8857	T8854	punct	],13
R5545	T8858	T8847	punct	", ",investigated
R5546	T8859	T8847	nsubj	we,investigated
R5547	T8726	T8717	dobj	place,took
R5548	T8860	T8861	det	the,status
R5549	T8861	T8847	dobj	status,investigated
R5550	T8727	T8717	prep	in,took
R5551	T8862	T8861	prep	of,status
R5552	T8863	T8864	compound	E,cadherin
R5553	T8864	T8862	pobj	cadherin,of
R5554	T8728	T8729	det	the,buds
R5555	T8865	T8864	punct	-,cadherin
R5556	T8866	T8847	prep	in,investigated
R5557	T8867	T8868	compound	TGF,β2
R5558	T8868	T8870	npadvmod	β2,null
R5559	T8869	T8868	punct	-,β2
R5560	T8870	T8872	amod	null,buds
R5561	T8729	T8727	pobj	buds,in
R5562	T8871	T8870	punct	-,null
R5563	T8872	T8866	pobj	buds,in
R5564	T8873	T8847	punct	.,investigated
R5565	T8730	T8731	compound	TGF,β2
R5566	T8875	T8876	mark	As,shown
R5567	T8731	T8733	npadvmod	β2,null
R5568	T8732	T8731	punct	-,β2
R5569	T8733	T8729	amod	null,buds
R5570	T8734	T8733	punct	-,null
R5571	T8876	T8877	advcl	shown,displayed
R5572	T8735	T8717	punct	", ",took
R5573	T8878	T8876	prep	in,shown
R5574	T8736	T8737	mark	as,judged
R5575	T8879	T8880	compound	Figure,6H
R5576	T8880	T8878	pobj	6H,in
R5577	T8881	T8877	punct	", ",displayed
R5578	T8737	T8717	advcl	judged,took
R5579	T8882	T8883	compound	hair,buds
R5580	T8883	T8877	nsubj	buds,displayed
R5581	T8884	T8883	prep	in,buds
R5582	T8738	T8737	prep	by,judged
R5583	T8885	T8886	compound	TGF,β2
R5584	T8886	T8888	npadvmod	β2,null
R5585	T8887	T8886	punct	-,β2
R5586	T8739	T8738	pobj	immunofluorescence,by
R5587	T8888	T8889	amod	null,skin
R5588	T8889	T8884	pobj	skin,in
R5589	T8740	T8739	prep	with,immunofluorescence
R5590	T8890	T8891	amod	elevated,staining
R5591	T8891	T8877	dobj	staining,displayed
R5592	T8892	T8891	compound	immunofluorescence,staining
R5593	T8741	T8740	pobj	antibodies,with
R5594	T8893	T8877	advcl	relative,displayed
R5595	T8894	T8893	prep	to,relative
R5596	T8895	T8896	poss	their,counterparts
R5597	T8742	T8741	prep	against,antibodies
R5598	T8896	T8894	pobj	counterparts,to
R5599	T8897	T8896	compound	WT,counterparts
R5600	T8898	T8877	punct	.,displayed
R5601	T8743	T8744	compound	β4,integrin
R5602	T8900	T8901	advmod	Previously,demonstrated
R5603	T8744	T8742	pobj	integrin,against
R5604	T8902	T8901	nsubj	we,demonstrated
R5605	T8903	T8904	mark	that,required
R5606	T8745	T8744	punct	", ",integrin
R5607	T8904	T8901	ccomp	required,demonstrated
R5608	T8905	T8906	det	the,action
R5609	T8906	T8904	nsubjpass	action,required
R5610	T8907	T8906	amod	concerted,action
R5611	T8908	T8906	prep	of,action
R5612	T8746	T8747	det	an,component
R5613	T8909	T8910	det	the,signals
R5614	T8910	T8908	pobj	signals,of
R5615	T8911	T8910	amod	extracellular,signals
R5616	T8747	T8744	appos	component,integrin
R5617	T8912	T8910	appos	Wnt,signals
R5618	T8913	T8912	cc	and,Wnt
R5619	T8914	T8912	conj	noggin,Wnt
R5620	T8748	T8747	amod	integral,component
R5621	T8915	T8904	auxpass	are,required
R5622	T8916	T8917	mark	for,repress
R5623	T8749	T8747	prep	of,component
R5624	T8750	T8751	npadvmod	keratinocyte,mediated
R5625	T8917	T8904	advcl	repress,required
R5626	T8918	T8919	det	the,generation
R5627	T8751	T8753	amod	mediated,adhesion
R5628	T8919	T8917	nsubj	generation,repress
R5629	T8920	T8919	prep	of,generation
R5630	T8921	T8922	det	a,complex
R5631	T8752	T8751	punct	-,mediated
R5632	T8922	T8920	pobj	complex,of
R5633	T8923	T8922	nmod	LEF,complex
R5634	T8924	T8923	punct	-,LEF
R5635	T8753	T8749	pobj	adhesion,of
R5636	T8925	T8923	nummod	1,LEF
R5637	T8926	T8923	punct	/,LEF
R5638	T8927	T8928	compound	β,catenin
R5639	T8754	T8753	prep	to,adhesion
R5640	T8928	T8923	appos	catenin,LEF
R5641	T8929	T8928	punct	-,catenin
R5642	T8755	T8756	poss	its,membrane
R5643	T8930	T8922	compound	transcription,complex
R5644	T8931	T8917	aux	to,repress
R5645	T8932	T8933	compound	E,cadherin
R5646	T8756	T8754	pobj	membrane,to
R5647	T8933	T8935	compound	cadherin,transcription
R5648	T8934	T8933	punct	-,cadherin
R5649	T8757	T8756	amod	underlying,membrane
R5650	T8935	T8917	dobj	transcription,repress
R5651	T8936	T8917	prep	at,repress
R5652	T8937	T8938	det	the,onset
R5653	T8758	T8756	compound	basement,membrane
R5654	T8938	T8936	pobj	onset,at
R5655	T8939	T8938	prep	of,onset
R5656	T8759	T8760	punct	(,6F
R5657	T8940	T8941	compound	hair,fate
R5658	T8941	T8942	compound	fate,specification
R5659	T8942	T8939	pobj	specification,of
R5660	T8943	T8901	punct	.,demonstrated
R5661	T8760	T8717	parataxis	6F,took
R5662	T8945	T8946	mark	As,shown
R5663	T8946	T8947	advcl	shown,exhibited
R5664	T8948	T8946	prep	in,shown
R5665	T8761	T8760	compound	Figure,6F
R5666	T8949	T8950	compound	Figure,6I
R5667	T8950	T8948	pobj	6I,in
R5668	T8762	T8760	punct	),6F
R5669	T8951	T8950	cc	and,6I
R5670	T8952	T8950	conj	6J,6I
R5671	T8953	T8947	punct	", ",exhibited
R5672	T8763	T8717	punct	.,took
R5673	T8954	T8955	preconj	both,WT
R5674	T8955	T8956	npadvmod	WT,null
R5675	T8765	T8766	prep	In,appeared
R5676	T8956	T8961	amod	null,buds
R5677	T8957	T8955	cc	and,WT
R5678	T8958	T8959	compound	TGF,β2
R5679	T8959	T8955	conj	β2,WT
R5680	T8960	T8959	punct	-,β2
R5681	T8767	T8765	pobj	contrast,In
R5682	T8961	T8947	nsubj	buds,exhibited
R5683	T8962	T8963	amod	nuclear,localization
R5684	T8963	T8947	dobj	localization,exhibited
R5685	T8964	T8963	nmod	LEF,localization
R5686	T8768	T8766	punct	", ",appeared
R5687	T8965	T8964	punct	-,LEF
R5688	T8966	T8964	nummod	1,LEF
R5689	T8967	T8964	cc	and,LEF
R5690	T8769	T8770	compound	TGF,β2
R5691	T8968	T8969	compound	β,catenin
R5692	T8969	T8964	conj	catenin,LEF
R5693	T8970	T8969	punct	-,catenin
R5694	T8982	T8947	punct	.,exhibited
R5695	T8971	T8947	punct	", ",exhibited
R5696	T8972	T8947	npadvmod	signs,exhibited
R5697	T8973	T8974	mark	that,was
R5698	T8974	T8972	acl	was,signs
R5699	T8975	T8976	det	the,pathway
R5700	T8976	T8974	nsubj	pathway,was
R5701	T8977	T8978	compound	Wnt,noggin
R5702	T8984	T8985	det	These,data
R5703	T8978	T8976	compound	noggin,pathway
R5704	T8979	T8978	punct	-,noggin
R5705	T8980	T8976	compound	signaling,pathway
R5706	T8981	T8974	acomp	intact,was
R5707	T8985	T8986	nsubj	data,suggest
R5708	T8987	T8988	mark	that,functions
R5709	T8988	T8986	ccomp	functions,suggest
R5710	T8989	T8988	prep	during,functions
R5711	T8990	T8991	compound	hair,follicle
R5712	T8991	T8992	compound	follicle,morphogenesis
R5713	T8992	T8989	pobj	morphogenesis,during
R5714	T8993	T8988	punct	", ",functions
R5715	T8994	T8995	compound	TGF,β2
R5716	T8995	T8988	nsubj	β2,functions
R5717	T8996	T8995	punct	-,β2
R5718	T8997	T8988	advmod	subsequently,functions
R5719	T8998	T8997	prep	to,subsequently
R5720	T8999	T9000	compound	Wnt,noggin
R5721	T9000	T9002	npadvmod	noggin,mediated
R5722	T9001	T9000	punct	/,noggin
R5723	T9002	T9004	amod	mediated,determination
R5724	T9003	T9002	punct	-,mediated
R5725	T9004	T8998	pobj	determination,to
R5726	T9005	T9004	prep	of,determination
R5727	T9006	T9007	compound	hair,fate
R5728	T9007	T9005	pobj	fate,of
R5729	T9008	T8986	punct	.,suggest
R5730	T9010	T9011	advmod	Moreover,appears
R5731	T9012	T9011	punct	", ",appears
R5732	T9013	T9011	prep	through,appears
R5733	T9014	T9013	pobj	activation,through
R5734	T9015	T9014	prep	of,activation
R5735	T9016	T9017	compound	Snail,expression
R5736	T9017	T9015	pobj	expression,of
R5737	T9018	T9017	compound	gene,expression
R5738	T9019	T9011	punct	", ",appears
R5739	T9020	T9021	compound	TGF,β2
R5740	T9021	T9011	nsubj	β2,appears
R5741	T9022	T9021	punct	-,β2
R5742	T9023	T9024	aux	to,work
R5743	T9024	T9011	xcomp	work,appears
R5744	T9025	T9024	prep	in,work
R5745	T9026	T9025	pobj	tandem,in
R5746	T9027	T9026	prep	with,tandem
R5747	T9028	T9029	det	these,morphogens
R5748	T9029	T9027	pobj	morphogens,with
R5749	T9030	T9029	amod	other,morphogens
R5750	T9031	T9032	aux	to,regulate
R5751	T9032	T9024	advcl	regulate,work
R5752	T9033	T9032	advmod	down,regulate
R5753	T9034	T9032	punct	-,regulate
R5754	T9035	T9036	compound	E,cadherin
R5755	T9036	T9038	compound	cadherin,levels
R5756	T9037	T9036	punct	-,cadherin
R5757	T9038	T9032	dobj	levels,regulate
R5758	T9039	T9024	punct	", ",work
R5759	T9040	T9041	dep	which,contributes
R5760	T9041	T9024	advcl	contributes,work
R5761	T9042	T9041	prep	to,contributes
R5762	T9043	T9044	det	the,activation
R5763	T9044	T9042	pobj	activation,to
R5764	T9045	T9044	prep	of,activation
R5765	T9046	T9047	amod	proliferative,circuitries
R5766	T9047	T9045	pobj	circuitries,of
R5767	T9048	T9011	punct	.,appears

PMC:539061 / 33902-45472 JSONTXT 5 Projects

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PMC:539061 / 33902-45472 JSON TXT 5 Projects