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Id Subject Object Predicate Lexical cue
T2682 0-7 JJ denotes Genetic
T2683 8-14 NN denotes System
T2684 15-18 IN denotes for
T2685 19-26 VBG denotes Testing
T2686 27-30 DT denotes the
T2687 31-39 NN denotes Function
T2688 40-42 IN denotes of
T2689 43-48 NNS denotes Genes
T2690 49-51 IN denotes in
T2691 52-57 NN denotes Joint
T2692 58-69 NN denotes Development
T2693 69-271 sentence denotes To generate a general system capable of specifically testing genes for functions in skeletal joint development, we engineered transgenic mice to express Cre recombinase in developing joints (Figure 1).
T2694 70-72 TO denotes To
T2695 73-81 VB denotes generate
T2697 82-83 DT denotes a
T2699 84-91 JJ denotes general
T2698 92-98 NN denotes system
T2700 99-106 JJ denotes capable
T2701 107-109 IN denotes of
T2702 110-122 RB denotes specifically
T2703 123-130 VBG denotes testing
T2704 131-136 NNS denotes genes
T2705 137-140 IN denotes for
T2706 141-150 NNS denotes functions
T2707 151-153 IN denotes in
T2708 154-162 JJ denotes skeletal
T2710 163-168 NN denotes joint
T2709 169-180 NN denotes development
T2711 180-182 , denotes ,
T2712 182-184 PRP denotes we
T2696 185-195 VBD denotes engineered
T2713 196-206 JJ denotes transgenic
T2714 207-211 NNS denotes mice
T2715 212-214 TO denotes to
T2716 215-222 VB denotes express
T2717 223-226 NN denotes Cre
T2718 227-238 NN denotes recombinase
T2719 239-241 IN denotes in
T2720 242-252 VBG denotes developing
T2721 253-259 NNS denotes joints
T2722 260-261 -LRB- denotes (
T2723 261-267 NN denotes Figure
T2724 268-269 CD denotes 1
T2725 269-270 -RRB- denotes )
T2726 270-271 . denotes .
T2727 271-386 sentence denotes Gdf5 is a gene strongly expressed in stripes across developing skeletal elements during embryonic joint formation.
T2728 272-276 NN denotes Gdf5
T2729 277-279 VBZ denotes is
T2730 280-281 DT denotes a
T2731 282-286 NN denotes gene
T2732 287-295 RB denotes strongly
T2733 296-305 VBN denotes expressed
T2734 306-308 IN denotes in
T2735 309-316 NNS denotes stripes
T2736 317-323 IN denotes across
T2737 324-334 VBG denotes developing
T2739 335-343 JJ denotes skeletal
T2738 344-352 NNS denotes elements
T2740 353-359 IN denotes during
T2741 360-369 JJ denotes embryonic
T2743 370-375 NN denotes joint
T2742 376-385 NN denotes formation
T2744 385-386 . denotes .
T2745 386-673 sentence denotes A bacterial artificial chromosome (BAC) containing the Gdf5 locus was modified by homologous recombination in bacteria to insert a cassette encoding Cre-internal ribosome entry site (IRES)-human placental alkaline phosphatase (hPLAP) into the translation start site of Gdf5 (Figure 1A).
T2746 387-388 DT denotes A
T2748 389-398 JJ denotes bacterial
T2749 399-409 JJ denotes artificial
T2747 410-420 NN denotes chromosome
T2751 421-422 -LRB- denotes (
T2752 422-425 NN denotes BAC
T2753 425-426 -RRB- denotes )
T2754 427-437 VBG denotes containing
T2755 438-441 DT denotes the
T2757 442-446 NN denotes Gdf5
T2756 447-452 NN denotes locus
T2758 453-456 VBD denotes was
T2750 457-465 VBN denotes modified
T2759 466-468 IN denotes by
T2760 469-479 JJ denotes homologous
T2761 480-493 NN denotes recombination
T2762 494-496 IN denotes in
T2763 497-505 NNS denotes bacteria
T2764 506-508 TO denotes to
T2765 509-515 VB denotes insert
T2766 516-517 DT denotes a
T2767 518-526 NN denotes cassette
T2768 527-535 VBG denotes encoding
T2769 536-539 NN denotes Cre
T2771 539-540 HYPH denotes -
T2772 540-548 JJ denotes internal
T2774 549-557 NN denotes ribosome
T2773 558-563 NN denotes entry
T2775 564-568 NN denotes site
T2776 569-570 -LRB- denotes (
T2777 570-574 NN denotes IRES
T2778 574-575 -RRB- denotes )
T2779 575-576 HYPH denotes -
T2780 576-581 JJ denotes human
T2781 582-591 JJ denotes placental
T2782 592-600 NN denotes alkaline
T2770 601-612 NN denotes phosphatase
T2783 613-614 -LRB- denotes (
T2784 614-619 NN denotes hPLAP
T2785 619-620 -RRB- denotes )
T2786 621-625 IN denotes into
T2787 626-629 DT denotes the
T2789 630-641 NN denotes translation
T2790 642-647 NN denotes start
T2788 648-652 NN denotes site
T2791 653-655 IN denotes of
T2792 656-660 NN denotes Gdf5
T2793 661-662 -LRB- denotes (
T2794 662-668 NN denotes Figure
T2795 669-671 CD denotes 1A
T2796 671-672 -RRB- denotes )
T2797 672-673 . denotes .
T2798 673-739 sentence denotes This modified BAC was then used to make lines of transgenic mice.
T2799 674-678 DT denotes This
T2801 679-687 VBN denotes modified
T2800 688-691 NN denotes BAC
T2803 692-695 VBD denotes was
T2804 696-700 RB denotes then
T2802 701-705 VBN denotes used
T2805 706-708 TO denotes to
T2806 709-713 VB denotes make
T2807 714-719 NNS denotes lines
T2808 720-722 IN denotes of
T2809 723-733 JJ denotes transgenic
T2810 734-738 NNS denotes mice
T2811 738-739 . denotes .
T2812 739-997 sentence denotes The resulting Gdf5-Cre transgenic mice were tested for transgene expression and Cre recombinase activity by crossing them to R26R reporter mice that activate the expression of lacZ after Cre-mediated removal of transcriptional stop sequences (Soriano 1999).
T2813 740-743 DT denotes The
T2815 744-753 VBG denotes resulting
T2816 754-758 NN denotes Gdf5
T2818 758-759 HYPH denotes -
T2817 759-762 NN denotes Cre
T2819 763-773 JJ denotes transgenic
T2814 774-778 NNS denotes mice
T2821 779-783 VBD denotes were
T2820 784-790 VBN denotes tested
T2822 791-794 IN denotes for
T2823 795-804 NN denotes transgene
T2824 805-815 NN denotes expression
T2825 816-819 CC denotes and
T2826 820-823 NN denotes Cre
T2827 824-835 NN denotes recombinase
T2828 836-844 NN denotes activity
T2829 845-847 IN denotes by
T2830 848-856 VBG denotes crossing
T2831 857-861 PRP denotes them
T2832 862-864 IN denotes to
T2833 865-869 NN denotes R26R
T2834 870-878 NN denotes reporter
T2835 879-883 NNS denotes mice
T2836 884-888 WDT denotes that
T2837 889-897 VBP denotes activate
T2838 898-901 DT denotes the
T2839 902-912 NN denotes expression
T2840 913-915 IN denotes of
T2841 916-920 NN denotes lacZ
T2842 921-926 IN denotes after
T2843 927-930 NN denotes Cre
T2845 930-931 HYPH denotes -
T2844 931-939 VBN denotes mediated
T2846 940-947 NN denotes removal
T2847 948-950 IN denotes of
T2848 951-966 JJ denotes transcriptional
T2850 967-971 NN denotes stop
T2849 972-981 NNS denotes sequences
T2851 982-983 -LRB- denotes (
T2852 983-990 NNP denotes Soriano
T2853 991-995 CD denotes 1999
T2854 995-996 -RRB- denotes )
T2855 996-997 . denotes .
T2856 997-1153 sentence denotes The resulting progeny were analyzed both for expression of the transgene by assaying HPLAP activity and for recombination of DNA by assaying LACZ activity.
T2857 998-1001 DT denotes The
T2859 1002-1011 VBG denotes resulting
T2858 1012-1019 NN denotes progeny
T2861 1020-1024 VBD denotes were
T2860 1025-1033 VBN denotes analyzed
T2862 1034-1038 CC denotes both
T2863 1039-1042 IN denotes for
T2864 1043-1053 NN denotes expression
T2865 1054-1056 IN denotes of
T2866 1057-1060 DT denotes the
T2867 1061-1070 NN denotes transgene
T2868 1071-1073 IN denotes by
T2869 1074-1082 VBG denotes assaying
T2870 1083-1088 NN denotes HPLAP
T2871 1089-1097 NN denotes activity
T2872 1098-1101 CC denotes and
T2873 1102-1105 IN denotes for
T2874 1106-1119 NN denotes recombination
T2875 1120-1122 IN denotes of
T2876 1123-1126 NN denotes DNA
T2877 1127-1129 IN denotes by
T2878 1130-1138 VBG denotes assaying
T2879 1139-1143 NN denotes LACZ
T2880 1144-1152 NN denotes activity
T2881 1152-1153 . denotes .
T2882 1153-1317 sentence denotes The progeny from all three lines showed strong LACZ expression primarily in joints, and in two of three lines HPLAP expression could also be seen in joint regions.
T2883 1154-1157 DT denotes The
T2884 1158-1165 NN denotes progeny
T2886 1166-1170 IN denotes from
T2887 1171-1174 DT denotes all
T2889 1175-1180 CD denotes three
T2888 1181-1186 NNS denotes lines
T2885 1187-1193 VBD denotes showed
T2890 1194-1200 JJ denotes strong
T2892 1201-1205 NN denotes LACZ
T2891 1206-1216 NN denotes expression
T2893 1217-1226 RB denotes primarily
T2894 1227-1229 IN denotes in
T2895 1230-1236 NNS denotes joints
T2896 1236-1238 , denotes ,
T2897 1238-1241 CC denotes and
T2898 1242-1244 IN denotes in
T2900 1245-1248 CD denotes two
T2902 1249-1251 IN denotes of
T2901 1252-1257 CD denotes three
T2903 1258-1263 NNS denotes lines
T2904 1264-1269 NN denotes HPLAP
T2905 1270-1280 NN denotes expression
T2906 1281-1286 MD denotes could
T2907 1287-1291 RB denotes also
T2908 1292-1294 VB denotes be
T2899 1295-1299 VBN denotes seen
T2909 1300-1302 IN denotes in
T2910 1303-1308 NN denotes joint
T2911 1309-1316 NNS denotes regions
T2912 1316-1317 . denotes .
T2913 1317-1555 sentence denotes Interestingly, HPLAP expression in the Gdf5-Cre transgenic GAC(A) line used for all subsequent breeding experiments was seen to precede LACZ expression during successive development of joints in the digits (Figure 1C) (unpublished data).
T2914 1318-1331 RB denotes Interestingly
T2916 1331-1333 , denotes ,
T2917 1333-1338 NN denotes HPLAP
T2918 1339-1349 NN denotes expression
T2919 1350-1352 IN denotes in
T2920 1353-1356 DT denotes the
T2922 1357-1361 NN denotes Gdf5
T2924 1361-1362 HYPH denotes -
T2923 1362-1365 NN denotes Cre
T2925 1366-1376 JJ denotes transgenic
T2926 1377-1380 NN denotes GAC
T2928 1380-1381 -LRB- denotes (
T2927 1381-1382 NN denotes A
T2929 1382-1383 -RRB- denotes )
T2921 1384-1388 NN denotes line
T2930 1389-1393 VBN denotes used
T2931 1394-1397 IN denotes for
T2932 1398-1401 DT denotes all
T2934 1402-1412 JJ denotes subsequent
T2935 1413-1421 NN denotes breeding
T2933 1422-1433 NNS denotes experiments
T2936 1434-1437 VBD denotes was
T2915 1438-1442 VBN denotes seen
T2937 1443-1445 TO denotes to
T2938 1446-1453 VB denotes precede
T2939 1454-1458 NN denotes LACZ
T2940 1459-1469 NN denotes expression
T2941 1470-1476 IN denotes during
T2942 1477-1487 JJ denotes successive
T2943 1488-1499 NN denotes development
T2944 1500-1502 IN denotes of
T2945 1503-1509 NNS denotes joints
T2946 1510-1512 IN denotes in
T2947 1513-1516 DT denotes the
T2948 1517-1523 NNS denotes digits
T2949 1524-1525 -LRB- denotes (
T2950 1525-1531 NN denotes Figure
T2951 1532-1534 CD denotes 1C
T2952 1534-1535 -RRB- denotes )
T2953 1536-1537 -LRB- denotes (
T2955 1537-1548 JJ denotes unpublished
T2954 1549-1553 NNS denotes data
T2956 1553-1554 -RRB- denotes )
T2957 1554-1555 . denotes .
T2958 1555-1705 sentence denotes These experiments clearly demonstrate that the Gdf5-Cre transgene expresses Cre recombinase and causes DNA recombination in developing joint regions.
T2959 1556-1561 DT denotes These
T2960 1562-1573 NNS denotes experiments
T2962 1574-1581 RB denotes clearly
T2961 1582-1593 VBP denotes demonstrate
T2963 1594-1598 IN denotes that
T2965 1599-1602 DT denotes the
T2967 1603-1607 NN denotes Gdf5
T2969 1607-1608 HYPH denotes -
T2968 1608-1611 NN denotes Cre
T2966 1612-1621 NN denotes transgene
T2964 1622-1631 VBZ denotes expresses
T2970 1632-1635 NN denotes Cre
T2971 1636-1647 NN denotes recombinase
T2972 1648-1651 CC denotes and
T2973 1652-1658 VBZ denotes causes
T2974 1659-1662 NN denotes DNA
T2975 1663-1676 NN denotes recombination
T2976 1677-1679 IN denotes in
T2977 1680-1690 VBG denotes developing
T2979 1691-1696 NN denotes joint
T2978 1697-1704 NNS denotes regions
T2980 1704-1705 . denotes .
T2981 1705-3524 sentence denotes Figure 1 A Genetic System to Drive Gene Recombination in Developing Joints (A) A 140-kb BAC from the Gdf5 locus was modified by inserting Cre-IRES-hPLAP into the translation start site of Gdf5 and used to make transgenic mice. Not to scale. See Materials and Methods for details. (B–E) Visualization of Gdf5-Cre driven recombination patterns based on activation of lacZ expression from the R26R Cre reporter allele. (B) LACZ activity is visible as blue staining in the ear (ea) and the joints of the shoulder (s), elbow (eb), wrist (w), knee (k), ankle (a), vertebra (vj), and phalanges (black arrowheads) of an E14.5 mouse embryo. (C) E14.5 hindlimb double-stained to show both HPLAP expression from the transgene (grey/purple staining) and LACZ expression from the rearranged R26R allele (blue staining). Note that both markers are visible in the oldest, proximal interphalangeal joint (black arrowhead), only HPLAP activity is visible in the more recently formed medial interphalangeal joint (black arrow), and neither HPLAP nor LACZ expression is visible in the youngest, most distal joint of the digit (white arrowhead). (D) Newborn (P0) forelimb with skin partially removed showing LACZ activity expressed in all phalangeal joints (red Salmon gal staining, black arrowheads) and regions of some tendons (asterisk). (E) Section through the most distal phalangeal joint of a P0 hindlimb stained with Alcian blue to mark cartilage showing LACZ expression (stained red) in all tissues of developing joints: articular cartilage (black arrowhead), precursors of ligaments and synovial membranes (black arrow), and cells where cavitation is occurring (asterisk). GAC(A) mice were crossed with lacZ ROSA26 Cre reporter strain (R26R) mice to analyze the pattern of Cre-mediated lacZ recombination throughout development.
T2982 3369-3372 NN denotes GAC
T2984 3372-3373 -LRB- denotes (
T2983 3373-3374 NN denotes A
T2986 3374-3375 -RRB- denotes )
T2985 3376-3380 NNS denotes mice
T2988 3381-3385 VBD denotes were
T2987 3386-3393 VBN denotes crossed
T2989 3394-3398 IN denotes with
T2990 3399-3403 NN denotes lacZ
T2992 3404-3410 NN denotes ROSA26
T2991 3411-3414 NN denotes Cre
T2993 3415-3423 NN denotes reporter
T2994 3424-3430 NN denotes strain
T2996 3431-3432 -LRB- denotes (
T2997 3432-3436 NN denotes R26R
T2998 3436-3437 -RRB- denotes )
T2995 3438-3442 NNS denotes mice
T2999 3443-3445 TO denotes to
T3000 3446-3453 VB denotes analyze
T3001 3454-3457 DT denotes the
T3002 3458-3465 NN denotes pattern
T3003 3466-3468 IN denotes of
T3004 3469-3472 NN denotes Cre
T3006 3472-3473 HYPH denotes -
T3005 3473-3481 VBN denotes mediated
T3008 3482-3486 NN denotes lacZ
T3007 3487-3500 NN denotes recombination
T3009 3501-3511 IN denotes throughout
T3010 3512-3523 NN denotes development
T3011 3523-3524 . denotes .
T3012 3524-3655 sentence denotes Joints in developing limbs begin forming in a proximal-distal pattern such that the shoulder joint forms prior to the elbow joint.
T3013 3525-3531 NNS denotes Joints
T3015 3532-3534 IN denotes in
T3016 3535-3545 VBG denotes developing
T3017 3546-3551 NNS denotes limbs
T3014 3552-3557 VBP denotes begin
T3018 3558-3565 VBG denotes forming
T3019 3566-3568 IN denotes in
T3020 3569-3570 DT denotes a
T3022 3571-3579 JJ denotes proximal
T3024 3579-3580 HYPH denotes -
T3023 3580-3586 JJ denotes distal
T3021 3587-3594 NN denotes pattern
T3025 3595-3599 JJ denotes such
T3027 3600-3604 IN denotes that
T3028 3605-3608 DT denotes the
T3030 3609-3617 NN denotes shoulder
T3029 3618-3623 NN denotes joint
T3026 3624-3629 VBZ denotes forms
T3031 3630-3635 RB denotes prior
T3032 3636-3638 IN denotes to
T3033 3639-3642 DT denotes the
T3035 3643-3648 NN denotes elbow
T3034 3649-3654 NN denotes joint
T3036 3654-3655 . denotes .
T3037 3655-3846 sentence denotes In addition, three major stages of early joint development have been defined by histology as (1) interzone formation, (2) three-layer interzone formation, and (3) cavitation (Mitrovic 1978).
T3038 3656-3658 IN denotes In
T3040 3659-3667 NN denotes addition
T3041 3667-3669 , denotes ,
T3042 3669-3674 CD denotes three
T3044 3675-3680 JJ denotes major
T3043 3681-3687 NNS denotes stages
T3045 3688-3690 IN denotes of
T3046 3691-3696 JJ denotes early
T3048 3697-3702 NN denotes joint
T3047 3703-3714 NN denotes development
T3049 3715-3719 VBP denotes have
T3050 3720-3724 VBN denotes been
T3039 3725-3732 VBN denotes defined
T3051 3733-3735 IN denotes by
T3052 3736-3745 NN denotes histology
T3053 3746-3748 IN denotes as
T3054 3749-3750 -LRB- denotes (
T3055 3750-3751 LS denotes 1
T3057 3751-3752 -RRB- denotes )
T3058 3753-3762 NN denotes interzone
T3056 3763-3772 NN denotes formation
T3059 3772-3774 , denotes ,
T3060 3774-3775 -LRB- denotes (
T3061 3775-3776 LS denotes 2
T3063 3776-3777 -RRB- denotes )
T3064 3778-3783 CD denotes three
T3066 3783-3784 HYPH denotes -
T3065 3784-3789 NN denotes layer
T3067 3790-3799 NN denotes interzone
T3062 3800-3809 NN denotes formation
T3068 3809-3811 , denotes ,
T3069 3811-3814 CC denotes and
T3070 3815-3816 -LRB- denotes (
T3071 3816-3817 LS denotes 3
T3073 3817-3818 -RRB- denotes )
T3072 3819-3829 NN denotes cavitation
T3074 3830-3831 -LRB- denotes (
T3075 3831-3839 NNP denotes Mitrovic
T3076 3840-3844 CD denotes 1978
T3077 3844-3845 -RRB- denotes )
T3078 3845-3846 . denotes .
T3079 3846-4058 sentence denotes Consistent with the proximal-distal pattern of joint development in the limbs, LACZ activity is seen at embryonic day 12.5 (E12.5) in the more proximal joints, including the shoulder and knee (unpublished data).
T3080 3847-3857 JJ denotes Consistent
T3082 3858-3862 IN denotes with
T3083 3863-3866 DT denotes the
T3085 3867-3875 JJ denotes proximal
T3087 3875-3876 HYPH denotes -
T3086 3876-3882 JJ denotes distal
T3084 3883-3890 NN denotes pattern
T3088 3891-3893 IN denotes of
T3089 3894-3899 NN denotes joint
T3090 3900-3911 NN denotes development
T3091 3912-3914 IN denotes in
T3092 3915-3918 DT denotes the
T3093 3919-3924 NNS denotes limbs
T3094 3924-3926 , denotes ,
T3095 3926-3930 NN denotes LACZ
T3096 3931-3939 NN denotes activity
T3097 3940-3942 VBZ denotes is
T3081 3943-3947 VBN denotes seen
T3098 3948-3950 IN denotes at
T3099 3951-3960 JJ denotes embryonic
T3100 3961-3964 NN denotes day
T3101 3965-3969 CD denotes 12.5
T3102 3970-3971 -LRB- denotes (
T3103 3971-3976 NN denotes E12.5
T3104 3976-3977 -RRB- denotes )
T3105 3978-3980 IN denotes in
T3106 3981-3984 DT denotes the
T3108 3985-3989 RBR denotes more
T3109 3990-3998 JJ denotes proximal
T3107 3999-4005 NNS denotes joints
T3110 4005-4007 , denotes ,
T3111 4007-4016 VBG denotes including
T3112 4017-4020 DT denotes the
T3113 4021-4029 NN denotes shoulder
T3114 4030-4033 CC denotes and
T3115 4034-4038 NN denotes knee
T3116 4039-4040 -LRB- denotes (
T3118 4040-4051 JJ denotes unpublished
T3117 4052-4056 NNS denotes data
T3119 4056-4057 -RRB- denotes )
T3120 4057-4058 . denotes .
T3121 4058-4260 sentence denotes By E14.5, LACZ expression is typically seen in all but the most distal joints of the limbs (Figure 1B and 1C), but with some variability in both strength and extent of expression from embryo to embryo.
T3122 4059-4061 IN denotes By
T3124 4062-4067 NN denotes E14.5
T3125 4067-4069 , denotes ,
T3126 4069-4073 NN denotes LACZ
T3127 4074-4084 NN denotes expression
T3128 4085-4087 VBZ denotes is
T3129 4088-4097 RB denotes typically
T3123 4098-4102 VBN denotes seen
T3130 4103-4105 IN denotes in
T3131 4106-4109 DT denotes all
T3132 4110-4113 IN denotes but
T3133 4114-4117 DT denotes the
T3135 4118-4122 RBS denotes most
T3136 4123-4129 JJ denotes distal
T3134 4130-4136 NNS denotes joints
T3137 4137-4139 IN denotes of
T3138 4140-4143 DT denotes the
T3139 4144-4149 NNS denotes limbs
T3140 4150-4151 -LRB- denotes (
T3142 4151-4157 NN denotes Figure
T3141 4158-4160 CD denotes 1B
T3143 4161-4164 CC denotes and
T3144 4165-4167 CD denotes 1C
T3145 4167-4168 -RRB- denotes )
T3146 4168-4170 , denotes ,
T3147 4170-4173 CC denotes but
T3148 4174-4178 IN denotes with
T3149 4179-4183 DT denotes some
T3150 4184-4195 NN denotes variability
T3151 4196-4198 IN denotes in
T3152 4199-4203 CC denotes both
T3153 4204-4212 NN denotes strength
T3154 4213-4216 CC denotes and
T3155 4217-4223 NN denotes extent
T3156 4224-4226 IN denotes of
T3157 4227-4237 NN denotes expression
T3158 4238-4242 IN denotes from
T3159 4243-4249 NN denotes embryo
T3160 4250-4252 IN denotes to
T3161 4253-4259 NN denotes embryo
T3162 4259-4260 . denotes .
T3163 4260-4443 sentence denotes The strongest-staining embryos often have additional staining in fingertips (not seen in the E14.5 embryo in Figure 1C, but clearly detectable in the E13.5 embryo shown in Figure 2).
T3164 4261-4264 DT denotes The
T3166 4265-4274 RBS denotes strongest
T3168 4274-4275 HYPH denotes -
T3167 4275-4283 VBG denotes staining
T3165 4284-4291 NNS denotes embryos
T3170 4292-4297 RB denotes often
T3169 4298-4302 VBP denotes have
T3171 4303-4313 JJ denotes additional
T3172 4314-4322 NN denotes staining
T3173 4323-4325 IN denotes in
T3174 4326-4336 NNS denotes fingertips
T3175 4337-4338 -LRB- denotes (
T3177 4338-4341 RB denotes not
T3176 4342-4346 VBN denotes seen
T3178 4347-4349 IN denotes in
T3179 4350-4353 DT denotes the
T3181 4354-4359 NN denotes E14.5
T3180 4360-4366 NN denotes embryo
T3182 4367-4369 IN denotes in
T3183 4370-4376 NN denotes Figure
T3184 4377-4379 CD denotes 1C
T3185 4379-4381 , denotes ,
T3186 4381-4384 CC denotes but
T3187 4385-4392 RB denotes clearly
T3188 4393-4403 JJ denotes detectable
T3189 4404-4406 IN denotes in
T3190 4407-4410 DT denotes the
T3192 4411-4416 NN denotes E13.5
T3191 4417-4423 NN denotes embryo
T3193 4424-4429 VBN denotes shown
T3194 4430-4432 IN denotes in
T3195 4433-4439 NN denotes Figure
T3196 4440-4441 CD denotes 2
T3197 4441-4442 -RRB- denotes )
T3198 4442-4443 . denotes .
T3199 4443-4561 sentence denotes Sections through developing joints show that LACZ is present in many cells at the interzone stage (unpublished data).
T3200 4444-4452 NNS denotes Sections
T3202 4453-4460 IN denotes through
T3203 4461-4471 VBG denotes developing
T3204 4472-4478 NNS denotes joints
T3201 4479-4483 VBP denotes show
T3205 4484-4488 IN denotes that
T3207 4489-4493 NN denotes LACZ
T3206 4494-4496 VBZ denotes is
T3208 4497-4504 JJ denotes present
T3209 4505-4507 IN denotes in
T3210 4508-4512 JJ denotes many
T3211 4513-4518 NNS denotes cells
T3212 4519-4521 IN denotes at
T3213 4522-4525 DT denotes the
T3215 4526-4535 NN denotes interzone
T3214 4536-4541 NN denotes stage
T3216 4542-4543 -LRB- denotes (
T3218 4543-4554 JJ denotes unpublished
T3217 4555-4559 NNS denotes data
T3219 4559-4560 -RRB- denotes )
T3220 4560-4561 . denotes .
T3221 4561-4778 sentence denotes However, expression of LACZ in nearly 100% of joint cells is not achieved until the three-layer interzone stage (for example, in the knee joint at E14.5 or in any of the phalangeal joints at E16.5 (unpublished data).
T3222 4562-4569 RB denotes However
T3224 4569-4571 , denotes ,
T3225 4571-4581 NN denotes expression
T3226 4582-4584 IN denotes of
T3227 4585-4589 NN denotes LACZ
T3228 4590-4592 IN denotes in
T3229 4593-4599 RB denotes nearly
T3230 4600-4603 CD denotes 100
T3231 4603-4604 NN denotes %
T3232 4605-4607 IN denotes of
T3233 4608-4613 NN denotes joint
T3234 4614-4619 NNS denotes cells
T3235 4620-4622 VBZ denotes is
T3236 4623-4626 RB denotes not
T3223 4627-4635 VBN denotes achieved
T3237 4636-4641 IN denotes until
T3238 4642-4645 DT denotes the
T3240 4646-4651 CD denotes three
T3242 4651-4652 HYPH denotes -
T3241 4652-4657 NN denotes layer
T3243 4658-4667 NN denotes interzone
T3239 4668-4673 NN denotes stage
T3244 4674-4675 -LRB- denotes (
T3245 4675-4678 IN denotes for
T3246 4679-4686 NN denotes example
T3247 4686-4688 , denotes ,
T3248 4688-4690 IN denotes in
T3249 4691-4694 DT denotes the
T3251 4695-4699 NN denotes knee
T3250 4700-4705 NN denotes joint
T3252 4706-4708 IN denotes at
T3253 4709-4714 NN denotes E14.5
T3254 4715-4717 CC denotes or
T3255 4718-4720 IN denotes in
T3256 4721-4724 DT denotes any
T3257 4725-4727 IN denotes of
T3258 4728-4731 DT denotes the
T3260 4732-4742 JJ denotes phalangeal
T3259 4743-4749 NNS denotes joints
T3261 4750-4752 IN denotes at
T3262 4753-4758 NN denotes E16.5
T3263 4759-4760 -LRB- denotes (
T3265 4760-4771 JJ denotes unpublished
T3264 4772-4776 NNS denotes data
T3266 4776-4777 -RRB- denotes )
T3267 4777-4778 . denotes .
T3268 4778-4904 sentence denotes Within the developing skeleton, Cre-mediated expression of LACZ remains strikingly specific to joints throughout development.
T3269 4779-4785 IN denotes Within
T3271 4786-4789 DT denotes the
T3273 4790-4800 VBG denotes developing
T3272 4801-4809 NN denotes skeleton
T3274 4809-4811 , denotes ,
T3275 4811-4814 NN denotes Cre
T3277 4814-4815 HYPH denotes -
T3276 4815-4823 VBN denotes mediated
T3278 4824-4834 NN denotes expression
T3279 4835-4837 IN denotes of
T3280 4838-4842 NN denotes LACZ
T3270 4843-4850 VBZ denotes remains
T3281 4851-4861 RB denotes strikingly
T3282 4862-4870 JJ denotes specific
T3283 4871-4873 IN denotes to
T3284 4874-4880 NNS denotes joints
T3285 4881-4891 IN denotes throughout
T3286 4892-4903 NN denotes development
T3287 4903-4904 . denotes .
T3288 4904-5090 sentence denotes Furthermore, it is seen in all the structures of postnatal synovial joints including the articular cartilage, joint capsule, and synovial membrane (Figure 1D and 1E) (unpublished data).
T3289 4905-4916 RB denotes Furthermore
T3291 4916-4918 , denotes ,
T3292 4918-4920 PRP denotes it
T3293 4921-4923 VBZ denotes is
T3290 4924-4928 VBN denotes seen
T3294 4929-4931 IN denotes in
T3295 4932-4935 PDT denotes all
T3297 4936-4939 DT denotes the
T3296 4940-4950 NNS denotes structures
T3298 4951-4953 IN denotes of
T3299 4954-4963 JJ denotes postnatal
T3301 4964-4972 JJ denotes synovial
T3300 4973-4979 NNS denotes joints
T3302 4980-4989 VBG denotes including
T3303 4990-4993 DT denotes the
T3305 4994-5003 JJ denotes articular
T3304 5004-5013 NN denotes cartilage
T3306 5013-5015 , denotes ,
T3307 5015-5020 NN denotes joint
T3308 5021-5028 NN denotes capsule
T3309 5028-5030 , denotes ,
T3310 5030-5033 CC denotes and
T3311 5034-5042 JJ denotes synovial
T3312 5043-5051 NN denotes membrane
T3313 5052-5053 -LRB- denotes (
T3314 5053-5059 NN denotes Figure
T3315 5060-5062 CD denotes 1D
T3316 5063-5066 CC denotes and
T3317 5067-5069 CD denotes 1E
T3318 5069-5070 -RRB- denotes )
T3319 5071-5072 -LRB- denotes (
T3321 5072-5083 JJ denotes unpublished
T3320 5084-5088 NNS denotes data
T3322 5088-5089 -RRB- denotes )
T3323 5089-5090 . denotes .
T3324 5090-5242 sentence denotes These patterns are consistent with the well-established expression of Gdf5 in interzone regions during embryonic development (Storm and Kingsley 1996).
T3325 5091-5096 DT denotes These
T3326 5097-5105 NNS denotes patterns
T3327 5106-5109 VBP denotes are
T3328 5110-5120 JJ denotes consistent
T3329 5121-5125 IN denotes with
T3330 5126-5129 DT denotes the
T3332 5130-5134 RB denotes well
T3334 5134-5135 HYPH denotes -
T3333 5135-5146 VBN denotes established
T3331 5147-5157 NN denotes expression
T3335 5158-5160 IN denotes of
T3336 5161-5165 NN denotes Gdf5
T3337 5166-5168 IN denotes in
T3338 5169-5178 NN denotes interzone
T3339 5179-5186 NNS denotes regions
T3340 5187-5193 IN denotes during
T3341 5194-5203 JJ denotes embryonic
T3342 5204-5215 NN denotes development
T3343 5216-5217 -LRB- denotes (
T3344 5217-5222 NNP denotes Storm
T3345 5223-5226 CC denotes and
T3346 5227-5235 NNP denotes Kingsley
T3347 5236-5240 CD denotes 1996
T3348 5240-5241 -RRB- denotes )
T3349 5241-5242 . denotes .
T3350 5242-5499 sentence denotes Adult expression patterns of the Gdf5 gene are not as well characterized, but Gdf5 expression has previously been detected in adult articular cartilage using both RT-PCR and immunocytochemistry (Chang et al. 1994; Erlacher et al. 1998; Bobacz et al. 2002).
T3351 5243-5248 NN denotes Adult
T3353 5249-5259 NN denotes expression
T3352 5260-5268 NNS denotes patterns
T3355 5269-5271 IN denotes of
T3356 5272-5275 DT denotes the
T3358 5276-5280 NN denotes Gdf5
T3357 5281-5285 NN denotes gene
T3354 5286-5289 VBP denotes are
T3359 5290-5293 RB denotes not
T3360 5294-5296 RB denotes as
T3362 5297-5301 RB denotes well
T3361 5302-5315 VBN denotes characterized
T3363 5315-5317 , denotes ,
T3364 5317-5320 CC denotes but
T3365 5321-5325 NN denotes Gdf5
T3366 5326-5336 NN denotes expression
T3368 5337-5340 VBZ denotes has
T3369 5341-5351 RB denotes previously
T3370 5352-5356 VBN denotes been
T3367 5357-5365 VBN denotes detected
T3371 5366-5368 IN denotes in
T3372 5369-5374 NN denotes adult
T3374 5375-5384 JJ denotes articular
T3373 5385-5394 NN denotes cartilage
T3375 5395-5400 VBG denotes using
T3376 5401-5405 CC denotes both
T3378 5406-5408 NN denotes RT
T3379 5408-5409 HYPH denotes -
T3377 5409-5412 NN denotes PCR
T3380 5413-5416 CC denotes and
T3381 5417-5436 NN denotes immunocytochemistry
T3382 5437-5438 -LRB- denotes (
T3383 5438-5443 NNP denotes Chang
T3384 5444-5446 FW denotes et
T3385 5447-5450 FW denotes al.
T3386 5451-5455 CD denotes 1994
T3387 5455-5456 : denotes ;
T3388 5457-5465 NNP denotes Erlacher
T3389 5466-5468 FW denotes et
T3390 5469-5472 FW denotes al.
T3391 5473-5477 CD denotes 1998
T3392 5477-5478 : denotes ;
T3393 5479-5485 NNP denotes Bobacz
T3394 5486-5488 FW denotes et
T3395 5489-5492 FW denotes al.
T3396 5493-5497 CD denotes 2002
T3397 5497-5498 -RRB- denotes )
T3398 5498-5499 . denotes .
T3399 5499-6971 sentence denotes Figure 2 Bmpr1a Is Required for Webbing Regression and Apoptosis in Specific Regions of the Limb (A and B) Control E14.5 forelimb (A) compared to a, E14.5 mutant forelimb (B) showing webbing between digits 1 and 2 (arrowheads) and extra tissue at the posterior of digit 5 (arrows). (C) Gdf5-Cre induced lacZ expression from R26R in an E13.5 forelimb showing LACZ staining (blue) in metacarpal-phalangeal joints, between digits 1 and 2 (arrowhead), and in a region posterior to digit 5 (arrow). (D and E) Sections of E14.5 hindlimbs showing apoptosis visualized by TUNEL staining (green) and proliferation visualized by staining for histone H3 phosphorylation (red). Controls show strong, uniform TUNEL staining between digits 1 and 2 (D, arrowhead) while mutants show patchy TUNEL staining interspersed with mitotic cells in similar regions (E). Scale bar = 200 μm. (F) Quantitation of TUNEL staining and mitotic cells in the posterior region of the fifth digit shows apoptosis is reduced 30% while proliferation is increased 20% (asterisks indicate statistically significant difference). (G and H) By E15.5, interdigital tissue has regressed in controls (G, arrowhead). In contrast, tissue remains in mutants at this location, primarily derived from cells that have undergone Gdf5-Cre-mediated recombination that inactivates Bmpr1a function and activates expression of LACZ (H). Scale bar = 75 μm. Other sites besides limb joints also have Cre-mediated lacZ expression.
T3400 6900-6905 JJ denotes Other
T3401 6906-6911 NNS denotes sites
T3403 6912-6919 IN denotes besides
T3404 6920-6924 NN denotes limb
T3405 6925-6931 NNS denotes joints
T3406 6932-6936 RB denotes also
T3402 6937-6941 VBP denotes have
T3407 6942-6945 NN denotes Cre
T3409 6945-6946 HYPH denotes -
T3408 6946-6954 VBN denotes mediated
T3411 6955-6959 NN denotes lacZ
T3410 6960-6970 NN denotes expression
T3412 6970-6971 . denotes .
T3413 6971-7081 sentence denotes Starting at E13.5, LACZ activity is detected in an anterior and posterior domain of the limb bud (Figure 2C).
T3414 6972-6980 VBG denotes Starting
T3416 6981-6983 IN denotes at
T3417 6984-6989 NN denotes E13.5
T3418 6989-6991 , denotes ,
T3419 6991-6995 NN denotes LACZ
T3420 6996-7004 NN denotes activity
T3421 7005-7007 VBZ denotes is
T3415 7008-7016 VBN denotes detected
T3422 7017-7019 IN denotes in
T3423 7020-7022 DT denotes an
T3425 7023-7031 JJ denotes anterior
T3426 7032-7035 CC denotes and
T3427 7036-7045 JJ denotes posterior
T3424 7046-7052 NN denotes domain
T3428 7053-7055 IN denotes of
T3429 7056-7059 DT denotes the
T3431 7060-7064 NN denotes limb
T3430 7065-7068 NN denotes bud
T3432 7069-7070 -LRB- denotes (
T3433 7070-7076 NN denotes Figure
T3434 7077-7079 CD denotes 2C
T3435 7079-7080 -RRB- denotes )
T3436 7080-7081 . denotes .
T3437 7081-7263 sentence denotes At E14.5, LACZ activity is detectable in the developing ear pinnae, ribs, sternum, tissues in the face, and some regions of the brain and spinal cord (Figure 1B) (unpublished data).
T3438 7082-7084 IN denotes At
T3440 7085-7090 NN denotes E14.5
T3441 7090-7092 , denotes ,
T3442 7092-7096 NN denotes LACZ
T3443 7097-7105 NN denotes activity
T3439 7106-7108 VBZ denotes is
T3444 7109-7119 JJ denotes detectable
T3445 7120-7122 IN denotes in
T3446 7123-7126 DT denotes the
T3448 7127-7137 VBG denotes developing
T3449 7138-7141 NN denotes ear
T3447 7142-7148 NNS denotes pinnae
T3450 7148-7150 , denotes ,
T3451 7150-7154 NNS denotes ribs
T3452 7154-7156 , denotes ,
T3453 7156-7163 NN denotes sternum
T3454 7163-7165 , denotes ,
T3455 7165-7172 NNS denotes tissues
T3456 7173-7175 IN denotes in
T3457 7176-7179 DT denotes the
T3458 7180-7184 NN denotes face
T3459 7184-7186 , denotes ,
T3460 7186-7189 CC denotes and
T3461 7190-7194 DT denotes some
T3462 7195-7202 NNS denotes regions
T3463 7203-7205 IN denotes of
T3464 7206-7209 DT denotes the
T3465 7210-7215 NN denotes brain
T3466 7216-7219 CC denotes and
T3467 7220-7226 JJ denotes spinal
T3468 7227-7231 NN denotes cord
T3469 7232-7233 -LRB- denotes (
T3470 7233-7239 NN denotes Figure
T3471 7240-7242 CD denotes 1B
T3472 7242-7243 -RRB- denotes )
T3473 7244-7245 -LRB- denotes (
T3475 7245-7256 JJ denotes unpublished
T3474 7257-7261 NNS denotes data
T3476 7261-7262 -RRB- denotes )
T3477 7262-7263 . denotes .
T3478 7263-7445 sentence denotes At birth, LACZ is also expressed in tendons running along the vertebral column, regions of tendons in the wrist and ankle, and some tendon insertions (Figure 1D) (unpublished data).
T3479 7264-7266 IN denotes At
T3481 7267-7272 NN denotes birth
T3482 7272-7274 , denotes ,
T3483 7274-7278 NN denotes LACZ
T3484 7279-7281 VBZ denotes is
T3485 7282-7286 RB denotes also
T3480 7287-7296 VBN denotes expressed
T3486 7297-7299 IN denotes in
T3487 7300-7307 NNS denotes tendons
T3488 7308-7315 VBG denotes running
T3489 7316-7321 IN denotes along
T3490 7322-7325 DT denotes the
T3492 7326-7335 JJ denotes vertebral
T3491 7336-7342 NN denotes column
T3493 7342-7344 , denotes ,
T3494 7344-7351 NNS denotes regions
T3495 7352-7354 IN denotes of
T3496 7355-7362 NNS denotes tendons
T3497 7363-7365 IN denotes in
T3498 7366-7369 DT denotes the
T3499 7370-7375 NN denotes wrist
T3500 7376-7379 CC denotes and
T3501 7380-7385 NN denotes ankle
T3502 7385-7387 , denotes ,
T3503 7387-7390 CC denotes and
T3504 7391-7395 DT denotes some
T3506 7396-7402 NN denotes tendon
T3505 7403-7413 NNS denotes insertions
T3507 7414-7415 -LRB- denotes (
T3508 7415-7421 NN denotes Figure
T3509 7422-7424 CD denotes 1D
T3510 7424-7425 -RRB- denotes )
T3511 7426-7427 -LRB- denotes (
T3513 7427-7438 JJ denotes unpublished
T3512 7439-7443 NNS denotes data
T3514 7443-7444 -RRB- denotes )
T3515 7444-7445 . denotes .
T3516 7445-7634 sentence denotes By 5 wk of age, LACZ is also expressed in the hair follicles, ear cartilage, some cells in the growth plate of the long bones, and portions of the brain and spinal cord (unpublished data).
T3517 7446-7448 IN denotes By
T3519 7449-7450 CD denotes 5
T3520 7451-7453 NNS denotes wk
T3521 7454-7456 IN denotes of
T3522 7457-7460 NN denotes age
T3523 7460-7462 , denotes ,
T3524 7462-7466 NN denotes LACZ
T3525 7467-7469 VBZ denotes is
T3526 7470-7474 RB denotes also
T3518 7475-7484 VBN denotes expressed
T3527 7485-7487 IN denotes in
T3528 7488-7491 DT denotes the
T3530 7492-7496 NN denotes hair
T3529 7497-7506 NNS denotes follicles
T3531 7506-7508 , denotes ,
T3532 7508-7511 NN denotes ear
T3533 7512-7521 NN denotes cartilage
T3534 7521-7523 , denotes ,
T3535 7523-7527 DT denotes some
T3536 7528-7533 NNS denotes cells
T3537 7534-7536 IN denotes in
T3538 7537-7540 DT denotes the
T3540 7541-7547 NN denotes growth
T3539 7548-7553 NN denotes plate
T3541 7554-7556 IN denotes of
T3542 7557-7560 DT denotes the
T3544 7561-7565 JJ denotes long
T3543 7566-7571 NNS denotes bones
T3545 7571-7573 , denotes ,
T3546 7573-7576 CC denotes and
T3547 7577-7585 NNS denotes portions
T3548 7586-7588 IN denotes of
T3549 7589-7592 DT denotes the
T3550 7593-7598 NN denotes brain
T3551 7599-7602 CC denotes and
T3552 7603-7609 JJ denotes spinal
T3553 7610-7614 NN denotes cord
T3554 7615-7616 -LRB- denotes (
T3556 7616-7627 JJ denotes unpublished
T3555 7628-7632 NNS denotes data
T3557 7632-7633 -RRB- denotes )
T3558 7633-7634 . denotes .
T3559 7634-7817 sentence denotes Surprisingly, 23 of 63, or 37% of transgenic mice analyzed also show some degree of wider “ectopic” LACZ expression, which can extend throughout many different tissues in the animal.
T3560 7635-7647 RB denotes Surprisingly
T3562 7647-7649 , denotes ,
T3563 7649-7651 CD denotes 23
T3565 7652-7654 IN denotes of
T3564 7655-7657 CD denotes 63
T3566 7657-7659 , denotes ,
T3567 7659-7661 CC denotes or
T3568 7662-7664 CD denotes 37
T3569 7664-7665 NN denotes %
T3570 7666-7668 IN denotes of
T3571 7669-7679 JJ denotes transgenic
T3572 7680-7684 NNS denotes mice
T3573 7685-7693 VBN denotes analyzed
T3574 7694-7698 RB denotes also
T3561 7699-7703 VBP denotes show
T3575 7704-7708 DT denotes some
T3576 7709-7715 NN denotes degree
T3577 7716-7718 IN denotes of
T3578 7719-7724 JJR denotes wider
T3580 7725-7726 `` denotes “
T3581 7726-7733 JJ denotes ectopic
T3582 7733-7734 '' denotes ”
T3583 7735-7739 NN denotes LACZ
T3579 7740-7750 NN denotes expression
T3584 7750-7752 , denotes ,
T3585 7752-7757 WDT denotes which
T3587 7758-7761 MD denotes can
T3586 7762-7768 VB denotes extend
T3588 7769-7779 IN denotes throughout
T3589 7780-7784 JJ denotes many
T3591 7785-7794 JJ denotes different
T3590 7795-7802 NNS denotes tissues
T3592 7803-7805 IN denotes in
T3593 7806-7809 DT denotes the
T3594 7810-7816 NN denotes animal
T3595 7816-7817 . denotes .
T3596 7817-8051 sentence denotes However, sustained expression of the transgene itself, as assayed by HPLAP activity, is still restricted primarily to joints in animals that show evidence of more generalized recombination based on LACZ expression (unpublished data).
T3597 7818-7825 RB denotes However
T3599 7825-7827 , denotes ,
T3600 7827-7836 VBN denotes sustained
T3601 7837-7847 NN denotes expression
T3602 7848-7850 IN denotes of
T3603 7851-7854 DT denotes the
T3604 7855-7864 NN denotes transgene
T3605 7865-7871 PRP denotes itself
T3606 7871-7873 , denotes ,
T3607 7873-7875 IN denotes as
T3608 7876-7883 VBN denotes assayed
T3609 7884-7886 IN denotes by
T3610 7887-7892 NN denotes HPLAP
T3611 7893-7901 NN denotes activity
T3612 7901-7903 , denotes ,
T3598 7903-7905 VBZ denotes is
T3613 7906-7911 RB denotes still
T3614 7912-7922 VBN denotes restricted
T3615 7923-7932 RB denotes primarily
T3616 7933-7935 IN denotes to
T3617 7936-7942 NNS denotes joints
T3618 7943-7945 IN denotes in
T3619 7946-7953 NNS denotes animals
T3620 7954-7958 WDT denotes that
T3621 7959-7963 VBP denotes show
T3622 7964-7972 NN denotes evidence
T3623 7973-7975 IN denotes of
T3624 7976-7980 RBR denotes more
T3625 7981-7992 VBN denotes generalized
T3626 7993-8006 NN denotes recombination
T3627 8007-8012 VBN denotes based
T3628 8013-8015 IN denotes on
T3629 8016-8020 NN denotes LACZ
T3630 8021-8031 NN denotes expression
T3631 8032-8033 -LRB- denotes (
T3633 8033-8044 JJ denotes unpublished
T3632 8045-8049 NNS denotes data
T3634 8049-8050 -RRB- denotes )
T3635 8050-8051 . denotes .
T3636 8051-8230 sentence denotes This suggests that in a fraction of animals, sporadic expression of Cre at some time early in development is sufficient to lead to both ectopic recombination and LACZ expression.
T3637 8052-8056 DT denotes This
T3638 8057-8065 VBZ denotes suggests
T3639 8066-8070 IN denotes that
T3641 8071-8073 IN denotes in
T3642 8074-8075 DT denotes a
T3643 8076-8084 NN denotes fraction
T3644 8085-8087 IN denotes of
T3645 8088-8095 NNS denotes animals
T3646 8095-8097 , denotes ,
T3647 8097-8105 JJ denotes sporadic
T3648 8106-8116 NN denotes expression
T3649 8117-8119 IN denotes of
T3650 8120-8123 NN denotes Cre
T3651 8124-8126 IN denotes at
T3652 8127-8131 DT denotes some
T3653 8132-8136 NN denotes time
T3654 8137-8142 RB denotes early
T3655 8143-8145 IN denotes in
T3656 8146-8157 NN denotes development
T3640 8158-8160 VBZ denotes is
T3657 8161-8171 JJ denotes sufficient
T3658 8172-8174 TO denotes to
T3659 8175-8179 VB denotes lead
T3660 8180-8182 IN denotes to
T3661 8183-8187 CC denotes both
T3663 8188-8195 JJ denotes ectopic
T3662 8196-8209 NN denotes recombination
T3664 8210-8213 CC denotes and
T3665 8214-8218 NN denotes LACZ
T3666 8219-8229 NN denotes expression
T3667 8229-8230 . denotes .
T3668 8230-8524 sentence denotes While the fraction of animals with broader recombination patterns must be tracked and accounted for during experiments, these animals offer the potential benefit of revealing additional new functions of target genes that could be subsequently studied with additional site-specific Cre drivers.
T3669 8231-8236 IN denotes While
T3671 8237-8240 DT denotes the
T3672 8241-8249 NN denotes fraction
T3673 8250-8252 IN denotes of
T3674 8253-8260 NNS denotes animals
T3675 8261-8265 IN denotes with
T3676 8266-8273 JJR denotes broader
T3678 8274-8287 NN denotes recombination
T3677 8288-8296 NNS denotes patterns
T3679 8297-8301 MD denotes must
T3680 8302-8304 VB denotes be
T3670 8305-8312 VBN denotes tracked
T3682 8313-8316 CC denotes and
T3683 8317-8326 VBN denotes accounted
T3684 8327-8330 IN denotes for
T3685 8331-8337 IN denotes during
T3686 8338-8349 NNS denotes experiments
T3687 8349-8351 , denotes ,
T3688 8351-8356 DT denotes these
T3689 8357-8364 NNS denotes animals
T3681 8365-8370 VBP denotes offer
T3690 8371-8374 DT denotes the
T3692 8375-8384 JJ denotes potential
T3691 8385-8392 NN denotes benefit
T3693 8393-8395 IN denotes of
T3694 8396-8405 VBG denotes revealing
T3695 8406-8416 JJ denotes additional
T3697 8417-8420 JJ denotes new
T3696 8421-8430 NNS denotes functions
T3698 8431-8433 IN denotes of
T3699 8434-8440 NN denotes target
T3700 8441-8446 NNS denotes genes
T3701 8447-8451 WDT denotes that
T3703 8452-8457 MD denotes could
T3704 8458-8460 VB denotes be
T3705 8461-8473 RB denotes subsequently
T3702 8474-8481 VBN denotes studied
T3706 8482-8486 IN denotes with
T3707 8487-8497 JJ denotes additional
T3709 8498-8502 NN denotes site
T3710 8502-8503 HYPH denotes -
T3711 8503-8511 JJ denotes specific
T3712 8512-8515 NN denotes Cre
T3708 8516-8523 NNS denotes drivers
T3713 8523-8524 . denotes .
R1427 T2682 T2683 amod Genetic,System
R1428 T2684 T2683 prep for,System
R1429 T2685 T2684 pcomp Testing,for
R1430 T2686 T2687 det the,Function
R1431 T2687 T2685 dobj Function,Testing
R1432 T2688 T2687 prep of,Function
R1433 T2689 T2688 pobj Genes,of
R1434 T2690 T2687 prep in,Function
R1435 T2691 T2692 compound Joint,Development
R1436 T2692 T2690 pobj Development,in
R1437 T2694 T2695 aux To,generate
R1438 T2695 T2696 advcl generate,engineered
R1439 T2697 T2698 det a,system
R1440 T2698 T2695 dobj system,generate
R1441 T2699 T2698 amod general,system
R1442 T2700 T2698 amod capable,system
R1443 T2701 T2700 prep of,capable
R1444 T2702 T2703 advmod specifically,testing
R1445 T2703 T2701 pcomp testing,of
R1446 T2704 T2703 dobj genes,testing
R1447 T2705 T2703 prep for,testing
R1448 T2706 T2705 pobj functions,for
R1449 T2707 T2706 prep in,functions
R1450 T2708 T2709 amod skeletal,development
R1451 T2709 T2707 pobj development,in
R1452 T2710 T2709 compound joint,development
R1453 T2711 T2696 punct ", ",engineered
R1454 T2712 T2696 nsubj we,engineered
R1455 T2713 T2714 amod transgenic,mice
R1456 T2714 T2696 dobj mice,engineered
R1457 T2715 T2716 aux to,express
R1458 T2716 T2696 xcomp express,engineered
R1459 T2717 T2718 compound Cre,recombinase
R1460 T2718 T2716 dobj recombinase,express
R1461 T2719 T2716 prep in,express
R1462 T2720 T2721 amod developing,joints
R1463 T2721 T2719 pobj joints,in
R1464 T2722 T2723 punct (,Figure
R1465 T2723 T2696 parataxis Figure,engineered
R1466 T2724 T2723 nummod 1,Figure
R1467 T2725 T2723 punct ),Figure
R1468 T2726 T2696 punct .,engineered
R1469 T2728 T2729 nsubj Gdf5,is
R1470 T2730 T2731 det a,gene
R1471 T2731 T2729 attr gene,is
R1472 T2732 T2733 advmod strongly,expressed
R1473 T2733 T2731 acl expressed,gene
R1474 T2734 T2733 prep in,expressed
R1475 T2735 T2734 pobj stripes,in
R1476 T2736 T2733 prep across,expressed
R1477 T2737 T2738 amod developing,elements
R1478 T2738 T2736 pobj elements,across
R1479 T2739 T2738 amod skeletal,elements
R1480 T2740 T2733 prep during,expressed
R1481 T2741 T2742 amod embryonic,formation
R1482 T2742 T2740 pobj formation,during
R1483 T2743 T2742 compound joint,formation
R1484 T2744 T2729 punct .,is
R1485 T2746 T2747 det A,chromosome
R1486 T2747 T2750 nsubjpass chromosome,modified
R1487 T2748 T2749 amod bacterial,artificial
R1488 T2749 T2747 amod artificial,chromosome
R1489 T2751 T2747 punct (,chromosome
R1490 T2752 T2747 appos BAC,chromosome
R1491 T2753 T2747 punct ),chromosome
R1492 T2754 T2747 acl containing,chromosome
R1493 T2755 T2756 det the,locus
R1494 T2756 T2754 dobj locus,containing
R1495 T2757 T2756 compound Gdf5,locus
R1496 T2758 T2750 auxpass was,modified
R1497 T2759 T2750 prep by,modified
R1498 T2760 T2761 amod homologous,recombination
R1499 T2761 T2759 pobj recombination,by
R1500 T2762 T2750 prep in,modified
R1501 T2763 T2762 pobj bacteria,in
R1502 T2764 T2765 aux to,insert
R1503 T2765 T2750 advcl insert,modified
R1504 T2766 T2767 det a,cassette
R1505 T2767 T2765 dobj cassette,insert
R1506 T2768 T2767 acl encoding,cassette
R1507 T2769 T2770 nmod Cre,phosphatase
R1508 T2770 T2768 dobj phosphatase,encoding
R1509 T2771 T2770 punct -,phosphatase
R1510 T2772 T2773 amod internal,entry
R1511 T2773 T2775 nmod entry,site
R1512 T2774 T2773 nmod ribosome,entry
R1513 T2775 T2770 nmod site,phosphatase
R1514 T2776 T2775 punct (,site
R1515 T2777 T2775 appos IRES,site
R1516 T2778 T2775 punct ),site
R1517 T2779 T2770 punct -,phosphatase
R1518 T2780 T2781 amod human,placental
R1519 T2781 T2770 amod placental,phosphatase
R1520 T2782 T2770 compound alkaline,phosphatase
R1521 T2783 T2770 punct (,phosphatase
R1522 T2784 T2770 appos hPLAP,phosphatase
R1523 T2785 T2770 punct ),phosphatase
R1524 T2786 T2765 prep into,insert
R1525 T2787 T2788 det the,site
R1526 T2788 T2786 pobj site,into
R1527 T2789 T2788 compound translation,site
R1528 T2790 T2788 compound start,site
R1529 T2791 T2788 prep of,site
R1530 T2792 T2791 pobj Gdf5,of
R1531 T2793 T2794 punct (,Figure
R1532 T2794 T2765 parataxis Figure,insert
R1533 T2795 T2794 nummod 1A,Figure
R1534 T2796 T2794 punct ),Figure
R1535 T2797 T2750 punct .,modified
R1536 T2799 T2800 det This,BAC
R1537 T2800 T2802 nsubjpass BAC,used
R1538 T2801 T2800 amod modified,BAC
R1539 T2803 T2802 auxpass was,used
R1540 T2804 T2802 advmod then,used
R1541 T2805 T2806 aux to,make
R1542 T2806 T2802 advcl make,used
R1543 T2807 T2806 dobj lines,make
R1544 T2808 T2807 prep of,lines
R1545 T2809 T2810 amod transgenic,mice
R1546 T2810 T2808 pobj mice,of
R1547 T2811 T2802 punct .,used
R1548 T2813 T2814 det The,mice
R1549 T2814 T2820 nsubjpass mice,tested
R1550 T2815 T2814 amod resulting,mice
R1551 T2816 T2817 nmod Gdf5,Cre
R1552 T2817 T2814 nmod Cre,mice
R1553 T2818 T2817 punct -,Cre
R1554 T2819 T2814 amod transgenic,mice
R1555 T2821 T2820 auxpass were,tested
R1556 T2822 T2820 prep for,tested
R1557 T2823 T2824 compound transgene,expression
R1558 T2824 T2822 pobj expression,for
R1559 T2825 T2824 cc and,expression
R1560 T2826 T2827 compound Cre,recombinase
R1561 T2827 T2828 compound recombinase,activity
R1562 T2828 T2824 conj activity,expression
R1563 T2829 T2820 prep by,tested
R1564 T2830 T2829 pcomp crossing,by
R1565 T2831 T2830 dobj them,crossing
R1566 T2832 T2830 prep to,crossing
R1567 T2833 T2834 compound R26R,reporter
R1568 T2834 T2835 compound reporter,mice
R1569 T2835 T2832 pobj mice,to
R1570 T2836 T2837 dep that,activate
R1571 T2837 T2835 relcl activate,mice
R1572 T2838 T2839 det the,expression
R1573 T2839 T2837 dobj expression,activate
R1574 T2840 T2839 prep of,expression
R1575 T2841 T2840 pobj lacZ,of
R1576 T2842 T2837 prep after,activate
R1577 T2843 T2844 npadvmod Cre,mediated
R1578 T2844 T2846 amod mediated,removal
R1579 T2845 T2844 punct -,mediated
R1580 T2846 T2842 pobj removal,after
R1581 T2847 T2846 prep of,removal
R1582 T2848 T2849 amod transcriptional,sequences
R1583 T2849 T2847 pobj sequences,of
R1584 T2850 T2849 compound stop,sequences
R1585 T2851 T2852 punct (,Soriano
R1586 T2852 T2820 meta Soriano,tested
R1587 T2853 T2852 nummod 1999,Soriano
R1588 T2854 T2852 punct ),Soriano
R1589 T2855 T2820 punct .,tested
R1590 T2857 T2858 det The,progeny
R1591 T2858 T2860 nsubjpass progeny,analyzed
R1592 T2859 T2858 amod resulting,progeny
R1593 T2861 T2860 auxpass were,analyzed
R1594 T2862 T2860 preconj both,analyzed
R1595 T2863 T2860 conj for,analyzed
R1596 T2864 T2863 pobj expression,for
R1597 T2865 T2864 prep of,expression
R1598 T2866 T2867 det the,transgene
R1599 T2867 T2865 pobj transgene,of
R1600 T2868 T2863 prep by,for
R1601 T2869 T2868 pcomp assaying,by
R1602 T2870 T2871 compound HPLAP,activity
R1603 T2871 T2869 dobj activity,assaying
R1604 T2872 T2860 cc and,analyzed
R1605 T2873 T2860 conj for,analyzed
R1606 T2874 T2873 pobj recombination,for
R1607 T2875 T2874 prep of,recombination
R1608 T2876 T2875 pobj DNA,of
R1609 T2877 T2873 prep by,for
R1610 T2878 T2877 pcomp assaying,by
R1611 T2879 T2880 compound LACZ,activity
R1612 T2880 T2878 dobj activity,assaying
R1613 T2881 T2860 punct .,analyzed
R1614 T2883 T2884 det The,progeny
R1615 T2884 T2885 nsubj progeny,showed
R1616 T2886 T2884 prep from,progeny
R1617 T2887 T2888 det all,lines
R1618 T2888 T2886 pobj lines,from
R1619 T2889 T2888 nummod three,lines
R1620 T2890 T2891 amod strong,expression
R1621 T2891 T2885 dobj expression,showed
R1622 T2892 T2891 compound LACZ,expression
R1623 T2893 T2894 advmod primarily,in
R1624 T2894 T2885 prep in,showed
R1625 T2895 T2894 pobj joints,in
R1626 T2896 T2885 punct ", ",showed
R1627 T2897 T2885 cc and,showed
R1628 T2898 T2899 prep in,seen
R1629 T2899 T2885 conj seen,showed
R1630 T2900 T2901 quantmod two,three
R1631 T2901 T2903 nummod three,lines
R1632 T2902 T2901 quantmod of,three
R1633 T2903 T2898 pobj lines,in
R1634 T2904 T2905 compound HPLAP,expression
R1635 T2905 T2899 nsubjpass expression,seen
R1636 T2906 T2899 aux could,seen
R1637 T2907 T2899 advmod also,seen
R1638 T2908 T2899 auxpass be,seen
R1639 T2909 T2899 prep in,seen
R1640 T2910 T2911 compound joint,regions
R1641 T2911 T2909 pobj regions,in
R1642 T2912 T2899 punct .,seen
R1643 T2914 T2915 advmod Interestingly,seen
R1644 T2916 T2915 punct ", ",seen
R1645 T2917 T2918 compound HPLAP,expression
R1646 T2918 T2915 nsubjpass expression,seen
R1647 T2919 T2918 prep in,expression
R1648 T2920 T2921 det the,line
R1649 T2921 T2919 pobj line,in
R1650 T2922 T2923 nmod Gdf5,Cre
R1651 T2923 T2921 nmod Cre,line
R1652 T2924 T2923 punct -,Cre
R1653 T2925 T2923 amod transgenic,Cre
R1654 T2926 T2927 nmod GAC,A
R1655 T2927 T2923 appos A,Cre
R1656 T2928 T2927 punct (,A
R1657 T2929 T2927 punct ),A
R1658 T2930 T2921 acl used,line
R1659 T2931 T2930 prep for,used
R1660 T2932 T2933 det all,experiments
R1661 T2933 T2931 pobj experiments,for
R1662 T2934 T2933 amod subsequent,experiments
R1663 T2935 T2933 compound breeding,experiments
R1664 T2936 T2915 auxpass was,seen
R1665 T2937 T2938 aux to,precede
R1666 T2938 T2915 xcomp precede,seen
R1667 T2939 T2940 compound LACZ,expression
R1668 T2940 T2938 dobj expression,precede
R1669 T2941 T2938 prep during,precede
R1670 T2942 T2943 amod successive,development
R1671 T2943 T2941 pobj development,during
R1672 T2944 T2943 prep of,development
R1673 T2945 T2944 pobj joints,of
R1674 T2946 T2943 prep in,development
R1675 T2947 T2948 det the,digits
R1676 T2948 T2946 pobj digits,in
R1677 T2949 T2950 punct (,Figure
R1678 T2950 T2915 parataxis Figure,seen
R1679 T2951 T2950 nummod 1C,Figure
R1680 T2952 T2950 punct ),Figure
R1681 T2953 T2954 punct (,data
R1682 T2954 T2915 meta data,seen
R1683 T2955 T2954 amod unpublished,data
R1684 T2956 T2954 punct ),data
R1685 T2957 T2915 punct .,seen
R1686 T2959 T2960 det These,experiments
R1687 T2960 T2961 nsubj experiments,demonstrate
R1688 T2962 T2961 advmod clearly,demonstrate
R1689 T2963 T2964 mark that,expresses
R1690 T2964 T2961 ccomp expresses,demonstrate
R1691 T2965 T2966 det the,transgene
R1692 T2966 T2964 nsubj transgene,expresses
R1693 T2967 T2968 compound Gdf5,Cre
R1694 T2968 T2966 compound Cre,transgene
R1695 T2969 T2968 punct -,Cre
R1696 T2970 T2971 compound Cre,recombinase
R1697 T2971 T2964 dobj recombinase,expresses
R1698 T2972 T2964 cc and,expresses
R1699 T2973 T2964 conj causes,expresses
R1700 T2974 T2975 compound DNA,recombination
R1701 T2975 T2973 dobj recombination,causes
R1702 T2976 T2964 prep in,expresses
R1703 T2977 T2978 amod developing,regions
R1704 T2978 T2976 pobj regions,in
R1705 T2979 T2978 compound joint,regions
R1706 T2980 T2961 punct .,demonstrate
R1707 T2982 T2983 nmod GAC,A
R1708 T2983 T2985 nmod A,mice
R1709 T2984 T2983 punct (,A
R1710 T2985 T2987 nsubjpass mice,crossed
R1711 T2986 T2983 punct ),A
R1712 T2988 T2987 auxpass were,crossed
R1713 T2989 T2987 prep with,crossed
R1714 T2990 T2991 nmod lacZ,Cre
R1715 T2991 T2993 nmod Cre,reporter
R1716 T2992 T2991 nmod ROSA26,Cre
R1717 T2993 T2994 nmod reporter,strain
R1718 T2994 T2995 nmod strain,mice
R1719 T2995 T2989 pobj mice,with
R1720 T2996 T2997 punct (,R26R
R1721 T2997 T2994 appos R26R,strain
R1722 T2998 T2997 punct ),R26R
R1723 T2999 T3000 aux to,analyze
R1724 T3000 T2987 advcl analyze,crossed
R1725 T3001 T3002 det the,pattern
R1726 T3002 T3000 dobj pattern,analyze
R1727 T3003 T3002 prep of,pattern
R1728 T3004 T3005 npadvmod Cre,mediated
R1729 T3005 T3007 amod mediated,recombination
R1730 T3006 T3005 punct -,mediated
R1731 T3007 T3003 pobj recombination,of
R1732 T3008 T3007 compound lacZ,recombination
R1733 T3009 T3000 prep throughout,analyze
R1734 T3010 T3009 pobj development,throughout
R1735 T3011 T2987 punct .,crossed
R1736 T3013 T3014 nsubj Joints,begin
R1737 T3015 T3013 prep in,Joints
R1738 T3016 T3017 amod developing,limbs
R1739 T3017 T3015 pobj limbs,in
R1740 T3018 T3014 xcomp forming,begin
R1741 T3019 T3018 prep in,forming
R1742 T3020 T3021 det a,pattern
R1743 T3021 T3019 pobj pattern,in
R1744 T3022 T3023 amod proximal,distal
R1745 T3023 T3021 amod distal,pattern
R1746 T3024 T3023 punct -,distal
R1747 T3025 T3026 amod such,forms
R1748 T3026 T3018 advcl forms,forming
R1749 T3027 T3026 mark that,forms
R1750 T3028 T3029 det the,joint
R1751 T3029 T3026 nsubj joint,forms
R1752 T3030 T3029 compound shoulder,joint
R1753 T3031 T3026 advmod prior,forms
R1754 T3032 T3031 prep to,prior
R1755 T3033 T3034 det the,joint
R1756 T3034 T3032 pobj joint,to
R1757 T3035 T3034 compound elbow,joint
R1758 T3036 T3014 punct .,begin
R1759 T3038 T3039 prep In,defined
R1760 T3040 T3038 pobj addition,In
R1761 T3041 T3039 punct ", ",defined
R1762 T3042 T3043 nummod three,stages
R1763 T3043 T3039 nsubjpass stages,defined
R1764 T3044 T3043 amod major,stages
R1765 T3045 T3043 prep of,stages
R1766 T3046 T3047 amod early,development
R1767 T3047 T3045 pobj development,of
R1768 T3048 T3047 compound joint,development
R1769 T3049 T3039 aux have,defined
R1770 T3050 T3039 auxpass been,defined
R1771 T3051 T3039 agent by,defined
R1772 T3052 T3051 pobj histology,by
R1773 T3053 T3039 prep as,defined
R1774 T3054 T3055 punct (,1
R1775 T3055 T3056 meta 1,formation
R1776 T3056 T3053 pobj formation,as
R1777 T3057 T3055 punct ),1
R1778 T3058 T3056 compound interzone,formation
R1779 T3059 T3056 punct ", ",formation
R1780 T3060 T3061 punct (,2
R1781 T3061 T3062 meta 2,formation
R1782 T3062 T3056 conj formation,formation
R1783 T3063 T3061 punct ),2
R1784 T3064 T3065 nummod three,layer
R1785 T3065 T3062 compound layer,formation
R1786 T3066 T3065 punct -,layer
R1787 T3067 T3062 compound interzone,formation
R1788 T3068 T3062 punct ", ",formation
R1789 T3069 T3062 cc and,formation
R1790 T3070 T3071 punct (,3
R1791 T3071 T3072 meta 3,cavitation
R1792 T3072 T3062 conj cavitation,formation
R1793 T3073 T3071 punct ),3
R1794 T3074 T3075 punct (,Mitrovic
R1795 T3075 T3039 meta Mitrovic,defined
R1796 T3076 T3075 nummod 1978,Mitrovic
R1797 T3077 T3075 punct ),Mitrovic
R1798 T3078 T3039 punct .,defined
R1799 T3080 T3081 advcl Consistent,seen
R1800 T3082 T3080 prep with,Consistent
R1801 T3083 T3084 det the,pattern
R1802 T3084 T3082 pobj pattern,with
R1803 T3085 T3086 amod proximal,distal
R1804 T3086 T3084 amod distal,pattern
R1805 T3087 T3086 punct -,distal
R1806 T3088 T3084 prep of,pattern
R1807 T3089 T3090 compound joint,development
R1808 T3090 T3088 pobj development,of
R1809 T3091 T3084 prep in,pattern
R1810 T3092 T3093 det the,limbs
R1811 T3093 T3091 pobj limbs,in
R1812 T3094 T3081 punct ", ",seen
R1813 T3095 T3096 compound LACZ,activity
R1814 T3096 T3081 nsubjpass activity,seen
R1815 T3097 T3081 auxpass is,seen
R1816 T3098 T3081 prep at,seen
R1817 T3099 T3100 amod embryonic,day
R1818 T3100 T3098 pobj day,at
R1819 T3101 T3100 nummod 12.5,day
R1820 T3102 T3100 punct (,day
R1821 T3103 T3100 appos E12.5,day
R1822 T3104 T3100 punct ),day
R1823 T3105 T3081 prep in,seen
R1824 T3106 T3107 det the,joints
R1825 T3107 T3105 pobj joints,in
R1826 T3108 T3109 advmod more,proximal
R1827 T3109 T3107 amod proximal,joints
R1828 T3110 T3107 punct ", ",joints
R1829 T3111 T3107 prep including,joints
R1830 T3112 T3113 det the,shoulder
R1831 T3113 T3111 pobj shoulder,including
R1832 T3114 T3113 cc and,shoulder
R1833 T3115 T3113 conj knee,shoulder
R1834 T3116 T3117 punct (,data
R1835 T3117 T3081 meta data,seen
R1836 T3118 T3117 amod unpublished,data
R1837 T3119 T3117 punct ),data
R1838 T3120 T3081 punct .,seen
R1839 T3122 T3123 prep By,seen
R1840 T3124 T3122 pobj E14.5,By
R1841 T3125 T3123 punct ", ",seen
R1842 T3126 T3127 compound LACZ,expression
R1843 T3127 T3123 nsubjpass expression,seen
R1844 T3128 T3123 auxpass is,seen
R1845 T3129 T3123 advmod typically,seen
R1846 T3130 T3123 prep in,seen
R1847 T3131 T3130 pobj all,in
R1848 T3132 T3131 prep but,all
R1849 T3133 T3134 det the,joints
R1850 T3134 T3132 pobj joints,but
R1851 T3135 T3136 advmod most,distal
R1852 T3136 T3134 amod distal,joints
R1853 T3137 T3134 prep of,joints
R1854 T3138 T3139 det the,limbs
R1855 T3139 T3137 pobj limbs,of
R1856 T3140 T3141 punct (,1B
R1857 T3141 T3123 parataxis 1B,seen
R1858 T3142 T3141 nmod Figure,1B
R1859 T3143 T3141 cc and,1B
R1860 T3144 T3141 conj 1C,1B
R1861 T3145 T3141 punct ),1B
R1862 T3146 T3123 punct ", ",seen
R1863 T3147 T3123 cc but,seen
R1864 T3148 T3147 prep with,but
R1865 T3149 T3150 det some,variability
R1866 T3150 T3148 pobj variability,with
R1867 T3151 T3150 prep in,variability
R1868 T3152 T3153 preconj both,strength
R1869 T3153 T3151 pobj strength,in
R1870 T3154 T3153 cc and,strength
R1871 T3155 T3153 conj extent,strength
R1872 T3156 T3153 prep of,strength
R1873 T3157 T3156 pobj expression,of
R1874 T3158 T3150 prep from,variability
R1875 T3159 T3158 pobj embryo,from
R1876 T3160 T3158 prep to,from
R1877 T3161 T3160 pobj embryo,to
R1878 T3162 T3123 punct .,seen
R1879 T3164 T3165 det The,embryos
R1880 T3165 T3169 nsubj embryos,have
R1881 T3166 T3167 advmod strongest,staining
R1882 T3167 T3165 amod staining,embryos
R1883 T3168 T3167 punct -,staining
R1884 T3170 T3169 advmod often,have
R1885 T3171 T3172 amod additional,staining
R1886 T3172 T3169 dobj staining,have
R1887 T3173 T3172 prep in,staining
R1888 T3174 T3173 pobj fingertips,in
R1889 T3175 T3176 punct (,seen
R1890 T3176 T3169 parataxis seen,have
R1891 T3177 T3176 neg not,seen
R1892 T3178 T3176 prep in,seen
R1893 T3179 T3180 det the,embryo
R1894 T3180 T3178 pobj embryo,in
R1895 T3181 T3180 compound E14.5,embryo
R1896 T3182 T3180 prep in,embryo
R1897 T3183 T3182 pobj Figure,in
R1898 T3184 T3183 nummod 1C,Figure
R1899 T3185 T3176 punct ", ",seen
R1900 T3186 T3176 cc but,seen
R1901 T3187 T3188 advmod clearly,detectable
R1902 T3188 T3176 conj detectable,seen
R1903 T3189 T3188 prep in,detectable
R1904 T3190 T3191 det the,embryo
R1905 T3191 T3189 pobj embryo,in
R1906 T3192 T3191 compound E13.5,embryo
R1907 T3193 T3191 acl shown,embryo
R1908 T3194 T3193 prep in,shown
R1909 T3195 T3194 pobj Figure,in
R1910 T3196 T3195 nummod 2,Figure
R1911 T3197 T3176 punct ),seen
R1912 T3198 T3169 punct .,have
R1913 T3200 T3201 nsubj Sections,show
R1914 T3202 T3200 prep through,Sections
R1915 T3203 T3204 amod developing,joints
R1916 T3204 T3202 pobj joints,through
R1917 T3205 T3206 mark that,is
R1918 T3206 T3201 ccomp is,show
R1919 T3207 T3206 nsubj LACZ,is
R1920 T3208 T3206 acomp present,is
R1921 T3209 T3206 prep in,is
R1922 T3210 T3211 amod many,cells
R1923 T3211 T3209 pobj cells,in
R1924 T3212 T3206 prep at,is
R1925 T3213 T3214 det the,stage
R1926 T3214 T3212 pobj stage,at
R1927 T3215 T3214 compound interzone,stage
R1928 T3216 T3217 punct (,data
R1929 T3217 T3201 meta data,show
R1930 T3218 T3217 amod unpublished,data
R1931 T3219 T3217 punct ),data
R1932 T3220 T3201 punct .,show
R1933 T3222 T3223 advmod However,achieved
R1934 T3224 T3223 punct ", ",achieved
R1935 T3225 T3223 nsubjpass expression,achieved
R1936 T3226 T3225 prep of,expression
R1937 T3227 T3226 pobj LACZ,of
R1938 T3228 T3225 prep in,expression
R1939 T3229 T3230 advmod nearly,100
R1940 T3230 T3231 nummod 100,%
R1941 T3231 T3228 pobj %,in
R1942 T3232 T3231 prep of,%
R1943 T3233 T3234 compound joint,cells
R1944 T3234 T3232 pobj cells,of
R1945 T3235 T3223 auxpass is,achieved
R1946 T3236 T3223 neg not,achieved
R1947 T3237 T3223 prep until,achieved
R1948 T3238 T3239 det the,stage
R1949 T3239 T3237 pobj stage,until
R1950 T3240 T3241 nummod three,layer
R1951 T3241 T3239 compound layer,stage
R1952 T3242 T3241 punct -,layer
R1953 T3243 T3239 compound interzone,stage
R1954 T3244 T3245 punct (,for
R1955 T3245 T3223 parataxis for,achieved
R1956 T3246 T3245 pobj example,for
R1957 T3247 T3245 punct ", ",for
R1958 T3248 T3245 conj in,for
R1959 T3249 T3250 det the,joint
R1960 T3250 T3248 pobj joint,in
R1961 T3251 T3250 compound knee,joint
R1962 T3252 T3250 prep at,joint
R1963 T3253 T3252 pobj E14.5,at
R1964 T3254 T3248 cc or,in
R1965 T3255 T3248 conj in,in
R1966 T3256 T3255 pobj any,in
R1967 T3257 T3256 prep of,any
R1968 T3258 T3259 det the,joints
R1969 T3259 T3257 pobj joints,of
R1970 T3260 T3259 amod phalangeal,joints
R1971 T3261 T3256 prep at,any
R1972 T3262 T3261 pobj E16.5,at
R1973 T3263 T3264 punct (,data
R1974 T3264 T3223 meta data,achieved
R1975 T3265 T3264 amod unpublished,data
R1976 T3266 T3264 punct ),data
R1977 T3267 T3223 punct .,achieved
R1978 T3269 T3270 prep Within,remains
R1979 T3271 T3272 det the,skeleton
R1980 T3272 T3269 pobj skeleton,Within
R1981 T3273 T3272 amod developing,skeleton
R1982 T3274 T3270 punct ", ",remains
R1983 T3275 T3276 npadvmod Cre,mediated
R1984 T3276 T3278 amod mediated,expression
R1985 T3277 T3276 punct -,mediated
R1986 T3278 T3270 nsubj expression,remains
R1987 T3279 T3278 prep of,expression
R1988 T3280 T3279 pobj LACZ,of
R1989 T3281 T3282 advmod strikingly,specific
R1990 T3282 T3270 oprd specific,remains
R1991 T3283 T3282 prep to,specific
R1992 T3284 T3283 pobj joints,to
R1993 T3285 T3270 prep throughout,remains
R1994 T3286 T3285 pobj development,throughout
R1995 T3287 T3270 punct .,remains
R1996 T3289 T3290 advmod Furthermore,seen
R1997 T3291 T3290 punct ", ",seen
R1998 T3292 T3290 nsubjpass it,seen
R1999 T3293 T3290 auxpass is,seen
R2000 T3294 T3290 prep in,seen
R2001 T3295 T3296 predet all,structures
R2002 T3296 T3294 pobj structures,in
R2003 T3297 T3296 det the,structures
R2004 T3298 T3296 prep of,structures
R2005 T3299 T3300 amod postnatal,joints
R2006 T3300 T3298 pobj joints,of
R2007 T3301 T3300 amod synovial,joints
R2008 T3302 T3296 prep including,structures
R2009 T3303 T3304 det the,cartilage
R2010 T3304 T3302 pobj cartilage,including
R2011 T3305 T3304 amod articular,cartilage
R2012 T3306 T3304 punct ", ",cartilage
R2013 T3307 T3308 compound joint,capsule
R2014 T3308 T3304 conj capsule,cartilage
R2015 T3309 T3308 punct ", ",capsule
R2016 T3310 T3308 cc and,capsule
R2017 T3311 T3312 amod synovial,membrane
R2018 T3312 T3308 conj membrane,capsule
R2019 T3313 T3314 punct (,Figure
R2020 T3314 T3290 parataxis Figure,seen
R2021 T3315 T3314 nummod 1D,Figure
R2022 T3316 T3314 cc and,Figure
R2023 T3317 T3314 conj 1E,Figure
R2024 T3318 T3314 punct ),Figure
R2025 T3319 T3320 punct (,data
R2026 T3320 T3290 meta data,seen
R2027 T3321 T3320 amod unpublished,data
R2028 T3322 T3320 punct ),data
R2029 T3323 T3290 punct .,seen
R2030 T3325 T3326 det These,patterns
R2031 T3326 T3327 nsubj patterns,are
R2032 T3328 T3327 acomp consistent,are
R2033 T3329 T3328 prep with,consistent
R2034 T3330 T3331 det the,expression
R2035 T3331 T3329 pobj expression,with
R2036 T3332 T3333 advmod well,established
R2037 T3333 T3331 amod established,expression
R2038 T3334 T3333 punct -,established
R2039 T3335 T3331 prep of,expression
R2040 T3336 T3335 pobj Gdf5,of
R2041 T3337 T3331 prep in,expression
R2042 T3338 T3339 compound interzone,regions
R2043 T3339 T3337 pobj regions,in
R2044 T3340 T3331 prep during,expression
R2045 T3341 T3342 amod embryonic,development
R2046 T3342 T3340 pobj development,during
R2047 T3343 T3344 punct (,Storm
R2048 T3344 T3327 meta Storm,are
R2049 T3345 T3344 cc and,Storm
R2050 T3346 T3344 conj Kingsley,Storm
R2051 T3347 T3346 nummod 1996,Kingsley
R2052 T3348 T3346 punct ),Kingsley
R2053 T3349 T3327 punct .,are
R2054 T3351 T3352 compound Adult,patterns
R2055 T3352 T3354 nsubj patterns,are
R2056 T3353 T3352 compound expression,patterns
R2057 T3355 T3352 prep of,patterns
R2058 T3356 T3357 det the,gene
R2059 T3357 T3355 pobj gene,of
R2060 T3358 T3357 compound Gdf5,gene
R2061 T3359 T3354 neg not,are
R2062 T3360 T3361 advmod as,characterized
R2063 T3361 T3354 acomp characterized,are
R2064 T3362 T3361 advmod well,characterized
R2065 T3363 T3354 punct ", ",are
R2066 T3364 T3354 cc but,are
R2067 T3365 T3366 compound Gdf5,expression
R2068 T3366 T3367 nsubjpass expression,detected
R2069 T3367 T3354 conj detected,are
R2070 T3368 T3367 aux has,detected
R2071 T3369 T3367 advmod previously,detected
R2072 T3370 T3367 auxpass been,detected
R2073 T3371 T3367 prep in,detected
R2074 T3372 T3373 nmod adult,cartilage
R2075 T3479 T3480 prep At,expressed
R2076 T3481 T3479 pobj birth,At
R2077 T3482 T3480 punct ", ",expressed
R2078 T3483 T3480 nsubjpass LACZ,expressed
R2079 T3484 T3480 auxpass is,expressed
R2080 T3373 T3371 pobj cartilage,in
R2081 T3485 T3480 advmod also,expressed
R2082 T3374 T3373 amod articular,cartilage
R2083 T3486 T3480 prep in,expressed
R2084 T3375 T3367 advcl using,detected
R2085 T3376 T3377 preconj both,PCR
R2086 T3377 T3375 dobj PCR,using
R2087 T3378 T3377 compound RT,PCR
R2088 T3379 T3377 punct -,PCR
R2089 T3487 T3486 pobj tendons,in
R2090 T3380 T3377 cc and,PCR
R2091 T3381 T3377 conj immunocytochemistry,PCR
R2092 T3382 T3383 punct (,Chang
R2093 T3383 T3367 meta Chang,detected
R2094 T3384 T3383 nmod et,Chang
R2095 T3488 T3487 acl running,tendons
R2096 T3385 T3383 nmod al.,Chang
R2097 T3386 T3383 nummod 1994,Chang
R2098 T3387 T3383 punct ;,Chang
R2099 T3489 T3488 prep along,running
R2100 T3388 T3383 nmod Erlacher,Chang
R2101 T3490 T3491 det the,column
R2102 T3491 T3489 pobj column,along
R2103 T3389 T3383 nmod et,Chang
R2104 T3390 T3383 nmod al.,Chang
R2105 T3391 T3383 nummod 1998,Chang
R2106 T3492 T3491 amod vertebral,column
R2107 T3392 T3383 punct ;,Chang
R2108 T3393 T3383 nmod Bobacz,Chang
R2109 T3493 T3487 punct ", ",tendons
R2110 T3394 T3383 nmod et,Chang
R2111 T3395 T3383 nmod al.,Chang
R2112 T3396 T3383 nummod 2002,Chang
R2113 T3494 T3487 conj regions,tendons
R2114 T3397 T3383 punct ),Chang
R2115 T3398 T3367 punct .,detected
R2116 T3495 T3494 prep of,regions
R2117 T3400 T3401 amod Other,sites
R2118 T3496 T3495 pobj tendons,of
R2119 T3401 T3402 nsubj sites,have
R2120 T3497 T3494 prep in,regions
R2121 T3403 T3401 prep besides,sites
R2122 T3404 T3405 compound limb,joints
R2123 T3498 T3499 det the,wrist
R2124 T3405 T3403 pobj joints,besides
R2125 T3406 T3402 advmod also,have
R2126 T3407 T3408 npadvmod Cre,mediated
R2127 T3408 T3410 amod mediated,expression
R2128 T3499 T3497 pobj wrist,in
R2129 T3409 T3408 punct -,mediated
R2130 T3410 T3402 dobj expression,have
R2131 T3411 T3410 compound lacZ,expression
R2132 T3500 T3499 cc and,wrist
R2133 T3412 T3402 punct .,have
R2134 T3414 T3415 advcl Starting,detected
R2135 T3501 T3499 conj ankle,wrist
R2136 T3416 T3414 prep at,Starting
R2137 T3502 T3494 punct ", ",regions
R2138 T3417 T3416 pobj E13.5,at
R2139 T3418 T3415 punct ", ",detected
R2140 T3419 T3420 compound LACZ,activity
R2141 T3420 T3415 nsubjpass activity,detected
R2142 T3421 T3415 auxpass is,detected
R2143 T3422 T3415 prep in,detected
R2144 T3423 T3424 det an,domain
R2145 T3503 T3494 cc and,regions
R2146 T3424 T3422 pobj domain,in
R2147 T3425 T3424 amod anterior,domain
R2148 T3426 T3425 cc and,anterior
R2149 T3427 T3425 conj posterior,anterior
R2150 T3504 T3505 det some,insertions
R2151 T3428 T3424 prep of,domain
R2152 T3429 T3430 det the,bud
R2153 T3505 T3494 conj insertions,regions
R2154 T3430 T3428 pobj bud,of
R2155 T3431 T3430 compound limb,bud
R2156 T3432 T3433 punct (,Figure
R2157 T3506 T3505 compound tendon,insertions
R2158 T3433 T3415 parataxis Figure,detected
R2159 T3434 T3433 nummod 2C,Figure
R2160 T3435 T3433 punct ),Figure
R2161 T3507 T3508 punct (,Figure
R2162 T3436 T3415 punct .,detected
R2163 T3438 T3439 prep At,is
R2164 T3508 T3480 parataxis Figure,expressed
R2165 T3440 T3438 pobj E14.5,At
R2166 T3441 T3439 punct ", ",is
R2167 T3509 T3508 nummod 1D,Figure
R2168 T3442 T3443 compound LACZ,activity
R2169 T3443 T3439 nsubj activity,is
R2170 T3510 T3508 punct ),Figure
R2171 T3444 T3439 acomp detectable,is
R2172 T3445 T3439 prep in,is
R2173 T3446 T3447 det the,pinnae
R2174 T3511 T3512 punct (,data
R2175 T3447 T3445 pobj pinnae,in
R2176 T3448 T3447 amod developing,pinnae
R2177 T3449 T3447 compound ear,pinnae
R2178 T3512 T3480 meta data,expressed
R2179 T3450 T3447 punct ", ",pinnae
R2180 T3451 T3447 appos ribs,pinnae
R2181 T3513 T3512 amod unpublished,data
R2182 T3452 T3447 punct ", ",pinnae
R2183 T3453 T3447 appos sternum,pinnae
R2184 T3454 T3447 punct ", ",pinnae
R2185 T3514 T3512 punct ),data
R2186 T3455 T3447 appos tissues,pinnae
R2187 T3456 T3439 prep in,is
R2188 T3457 T3458 det the,face
R2189 T3515 T3480 punct .,expressed
R2190 T3458 T3456 pobj face,in
R2191 T3459 T3458 punct ", ",face
R2192 T3460 T3458 cc and,face
R2193 T3461 T3462 det some,regions
R2194 T3462 T3458 conj regions,face
R2195 T3463 T3462 prep of,regions
R2196 T3517 T3518 prep By,expressed
R2197 T3464 T3465 det the,brain
R2198 T3465 T3463 pobj brain,of
R2199 T3466 T3465 cc and,brain
R2200 T3467 T3468 amod spinal,cord
R2201 T3468 T3465 conj cord,brain
R2202 T3519 T3520 nummod 5,wk
R2203 T3469 T3470 punct (,Figure
R2204 T3470 T3439 parataxis Figure,is
R2205 T3471 T3470 nummod 1B,Figure
R2206 T3472 T3470 punct ),Figure
R2207 T3473 T3474 punct (,data
R2208 T3520 T3517 pobj wk,By
R2209 T3474 T3439 meta data,is
R2210 T3475 T3474 amod unpublished,data
R2211 T3521 T3520 prep of,wk
R2212 T3476 T3474 punct ),data
R2213 T3522 T3521 pobj age,of
R2214 T3477 T3439 punct .,is
R2215 T3523 T3518 punct ", ",expressed
R2216 T3524 T3518 nsubjpass LACZ,expressed
R2217 T3525 T3518 auxpass is,expressed
R2218 T3526 T3518 advmod also,expressed
R2219 T3527 T3518 prep in,expressed
R2220 T3528 T3529 det the,follicles
R2221 T3529 T3527 pobj follicles,in
R2222 T3530 T3529 compound hair,follicles
R2223 T3585 T3586 dep which,extend
R2224 T3531 T3529 punct ", ",follicles
R2225 T3586 T3579 relcl extend,expression
R2226 T3587 T3586 aux can,extend
R2227 T3588 T3586 prep throughout,extend
R2228 T3532 T3533 compound ear,cartilage
R2229 T3589 T3590 amod many,tissues
R2230 T3590 T3588 pobj tissues,throughout
R2231 T3591 T3590 amod different,tissues
R2232 T3533 T3529 appos cartilage,follicles
R2233 T3592 T3590 prep in,tissues
R2234 T3593 T3594 det the,animal
R2235 T3594 T3592 pobj animal,in
R2236 T3534 T3529 punct ", ",follicles
R2237 T3595 T3561 punct .,show
R2238 T3535 T3536 det some,cells
R2239 T3597 T3598 advmod However,is
R2240 T3599 T3598 punct ", ",is
R2241 T3536 T3529 conj cells,follicles
R2242 T3600 T3601 amod sustained,expression
R2243 T3601 T3598 nsubj expression,is
R2244 T3602 T3601 prep of,expression
R2245 T3603 T3604 det the,transgene
R2246 T3604 T3602 pobj transgene,of
R2247 T3605 T3604 appos itself,transgene
R2248 T3537 T3536 prep in,cells
R2249 T3606 T3598 punct ", ",is
R2250 T3607 T3608 mark as,assayed
R2251 T3608 T3598 advcl assayed,is
R2252 T3538 T3539 det the,plate
R2253 T3609 T3608 prep by,assayed
R2254 T3610 T3611 compound HPLAP,activity
R2255 T3611 T3609 pobj activity,by
R2256 T3539 T3537 pobj plate,in
R2257 T3612 T3598 punct ", ",is
R2258 T3613 T3598 advmod still,is
R2259 T3614 T3598 acomp restricted,is
R2260 T3540 T3539 compound growth,plate
R2261 T3615 T3616 advmod primarily,to
R2262 T3616 T3614 prep to,restricted
R2263 T3617 T3616 pobj joints,to
R2264 T3541 T3539 prep of,plate
R2265 T3618 T3617 prep in,joints
R2266 T3619 T3618 pobj animals,in
R2267 T3620 T3621 dep that,show
R2268 T3542 T3543 det the,bones
R2269 T3621 T3619 relcl show,animals
R2270 T3622 T3621 dobj evidence,show
R2271 T3623 T3622 prep of,evidence
R2272 T3543 T3541 pobj bones,of
R2273 T3624 T3625 advmod more,generalized
R2274 T3625 T3626 amod generalized,recombination
R2275 T3626 T3623 pobj recombination,of
R2276 T3544 T3543 amod long,bones
R2277 T3627 T3621 prep based,show
R2278 T3628 T3627 prep on,based
R2279 T3629 T3630 compound LACZ,expression
R2280 T3545 T3536 punct ", ",cells
R2281 T3630 T3628 pobj expression,on
R2282 T3631 T3632 punct (,data
R2283 T3632 T3598 meta data,is
R2284 T3546 T3536 cc and,cells
R2285 T3633 T3632 amod unpublished,data
R2286 T3634 T3632 punct ),data
R2287 T3635 T3598 punct .,is
R2288 T3547 T3536 conj portions,cells
R2289 T3637 T3638 nsubj This,suggests
R2290 T3548 T3547 prep of,portions
R2291 T3639 T3640 mark that,is
R2292 T3549 T3550 det the,brain
R2293 T3550 T3548 pobj brain,of
R2294 T3640 T3638 ccomp is,suggests
R2295 T3641 T3640 prep in,is
R2296 T3642 T3643 det a,fraction
R2297 T3643 T3641 pobj fraction,in
R2298 T3644 T3643 prep of,fraction
R2299 T3551 T3550 cc and,brain
R2300 T3645 T3644 pobj animals,of
R2301 T3646 T3640 punct ", ",is
R2302 T3647 T3648 amod sporadic,expression
R2303 T3552 T3553 amod spinal,cord
R2304 T3648 T3640 nsubj expression,is
R2305 T3649 T3648 prep of,expression
R2306 T3650 T3649 pobj Cre,of
R2307 T3553 T3550 conj cord,brain
R2308 T3651 T3648 prep at,expression
R2309 T3652 T3653 det some,time
R2310 T3653 T3651 pobj time,at
R2311 T3554 T3555 punct (,data
R2312 T3654 T3653 advmod early,time
R2313 T3655 T3654 prep in,early
R2314 T3656 T3655 pobj development,in
R2315 T3555 T3547 meta data,portions
R2316 T3657 T3640 acomp sufficient,is
R2317 T3658 T3659 aux to,lead
R2318 T3556 T3555 amod unpublished,data
R2319 T3659 T3657 xcomp lead,sufficient
R2320 T3660 T3659 prep to,lead
R2321 T3661 T3662 preconj both,recombination
R2322 T3557 T3555 punct ),data
R2323 T3662 T3660 pobj recombination,to
R2324 T3663 T3662 amod ectopic,recombination
R2325 T3664 T3662 cc and,recombination
R2326 T3558 T3518 punct .,expressed
R2327 T3665 T3666 compound LACZ,expression
R2328 T3666 T3662 conj expression,recombination
R2329 T3667 T3638 punct .,suggests
R2330 T3560 T3561 advmod Surprisingly,show
R2331 T3669 T3670 mark While,tracked
R2332 T3670 T3681 advcl tracked,offer
R2333 T3671 T3672 det the,fraction
R2334 T3562 T3561 punct ", ",show
R2335 T3672 T3670 nsubj fraction,tracked
R2336 T3673 T3672 prep of,fraction
R2337 T3674 T3673 pobj animals,of
R2338 T3563 T3564 quantmod 23,63
R2339 T3675 T3674 prep with,animals
R2340 T3676 T3677 amod broader,patterns
R2341 T3677 T3675 pobj patterns,with
R2342 T3564 T3561 nsubj 63,show
R2343 T3678 T3677 compound recombination,patterns
R2344 T3679 T3670 aux must,tracked
R2345 T3680 T3670 aux be,tracked
R2346 T3682 T3670 cc and,tracked
R2347 T3683 T3670 conj accounted,tracked
R2348 T3565 T3564 quantmod of,63
R2349 T3684 T3683 prep for,accounted
R2350 T3685 T3670 prep during,tracked
R2351 T3686 T3685 pobj experiments,during
R2352 T3566 T3564 punct ", ",63
R2353 T3687 T3681 punct ", ",offer
R2354 T3688 T3689 det these,animals
R2355 T3689 T3681 nsubj animals,offer
R2356 T3567 T3564 cc or,63
R2357 T3690 T3691 det the,benefit
R2358 T3568 T3569 nummod 37,%
R2359 T3569 T3564 conj %,63
R2360 T3691 T3681 dobj benefit,offer
R2361 T3570 T3564 prep of,63
R2362 T3692 T3691 amod potential,benefit
R2363 T3693 T3691 prep of,benefit
R2364 T3694 T3693 pcomp revealing,of
R2365 T3571 T3572 amod transgenic,mice
R2366 T3695 T3696 amod additional,functions
R2367 T3696 T3694 dobj functions,revealing
R2368 T3697 T3696 amod new,functions
R2369 T3572 T3570 pobj mice,of
R2370 T3698 T3696 prep of,functions
R2371 T3699 T3700 compound target,genes
R2372 T3700 T3698 pobj genes,of
R2373 T3573 T3572 acl analyzed,mice
R2374 T3701 T3702 dep that,studied
R2375 T3702 T3700 relcl studied,genes
R2376 T3703 T3702 aux could,studied
R2377 T3574 T3561 advmod also,show
R2378 T3704 T3702 auxpass be,studied
R2379 T3705 T3702 advmod subsequently,studied
R2380 T3706 T3702 prep with,studied
R2381 T3575 T3576 det some,degree
R2382 T3707 T3708 amod additional,drivers
R2383 T3708 T3706 pobj drivers,with
R2384 T3709 T3708 nmod site,drivers
R2385 T3576 T3561 dobj degree,show
R2386 T3710 T3709 punct -,site
R2387 T3711 T3709 amod specific,site
R2388 T3712 T3708 compound Cre,drivers
R2389 T3577 T3576 prep of,degree
R2390 T3713 T3681 punct .,offer
R2391 T3578 T3579 amod wider,expression
R2392 T3579 T3577 pobj expression,of
R2393 T3580 T3579 punct “,expression
R2394 T3581 T3579 amod ectopic,expression
R2395 T3582 T3579 punct ”,expression
R2396 T3583 T3579 compound LACZ,expression
R2397 T3584 T3579 punct ", ",expression