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PMC:340383 / 19050-19658 JSONTXT 5 Projects

Annnotations TAB TSV DIC JSON TextAE

Id Subject Object Predicate Lexical cue
T4651 0-8 JJ denotes Multiple
T4653 9-15 NN denotes Choice
T4654 16-24 NNP denotes Northern
T4655 25-29 NN denotes Blot
T4652 30-37 NNS denotes filters
T4657 38-48 VBG denotes containing
T4658 49-51 CD denotes 12
T4660 52-61 JJ denotes different
T4661 62-67 NN denotes mouse
T4659 68-75 NNS denotes tissues
T4662 76-80 VBD denotes were
T4656 81-90 VBN denotes purchased
T4663 91-95 IN denotes from
T4664 96-103 NNP denotes Origene
T4665 103-104 . denotes .
T4666 104-315 sentence denotes The filters were probed for each Acdp gene with a PCR fragment (around 350 bp) from last exon and the 3' untranslated region labeled with α-32P dCTP using the random primer extension system (Life Technologies).
T4667 105-108 DT denotes The
T4668 109-116 NNS denotes filters
T4670 117-121 VBD denotes were
T4669 122-128 VBN denotes probed
T4671 129-132 IN denotes for
T4672 133-137 DT denotes each
T4674 138-142 NN denotes Acdp
T4673 143-147 NN denotes gene
T4675 148-152 IN denotes with
T4676 153-154 DT denotes a
T4678 155-158 NN denotes PCR
T4677 159-167 NN denotes fragment
T4679 168-169 -LRB- denotes (
T4681 169-175 IN denotes around
T4682 176-179 CD denotes 350
T4680 180-182 NN denotes bp
T4683 182-183 -RRB- denotes )
T4684 184-188 IN denotes from
T4685 189-193 JJ denotes last
T4686 194-198 NN denotes exon
T4687 199-202 CC denotes and
T4688 203-206 DT denotes the
T4689 207-208 CD denotes 3
T4691 208-209 SYM denotes '
T4692 210-222 JJ denotes untranslated
T4690 223-229 NN denotes region
T4693 230-237 VBN denotes labeled
T4694 238-242 IN denotes with
T4695 243-244 NN denotes α
T4697 244-245 HYPH denotes -
T4696 245-248 NN denotes 32P
T4698 249-253 NN denotes dCTP
T4699 254-259 VBG denotes using
T4700 260-263 DT denotes the
T4702 264-270 JJ denotes random
T4703 271-277 NN denotes primer
T4704 278-287 NN denotes extension
T4701 288-294 NN denotes system
T4705 295-296 -LRB- denotes (
T4707 296-300 NNP denotes Life
T4706 301-313 NNP denotes Technologies
T4708 313-314 -RRB- denotes )
T4709 314-315 . denotes .
T4710 315-358 sentence denotes Hybridizations were carried out overnight.
T4711 316-330 NNS denotes Hybridizations
T4713 331-335 VBD denotes were
T4712 336-343 VBN denotes carried
T4714 344-347 RP denotes out
T4715 348-357 RB denotes overnight
T4716 357-358 . denotes .
T4717 358-534 sentence denotes The filters were washed twice with washing buffer I (2×SSC, 0.1% SDS) at 42°C for 15-min, and then washed twice with washing buffer II (0.25×SSC, 0.1% SDS) at 65°C for 15-min.
T4718 359-362 DT denotes The
T4719 363-370 NNS denotes filters
T4721 371-375 VBD denotes were
T4720 376-382 VBN denotes washed
T4722 383-388 RB denotes twice
T4723 389-393 IN denotes with
T4724 394-401 VBG denotes washing
T4725 402-408 NN denotes buffer
T4726 409-410 CD denotes I
T4727 411-412 -LRB- denotes (
T4729 412-413 CD denotes 2
T4731 413-414 SYM denotes ×
T4730 414-417 NN denotes SSC
T4732 417-419 , denotes ,
T4733 419-422 CD denotes 0.1
T4734 422-423 NN denotes %
T4728 424-427 NN denotes SDS
T4735 427-428 -RRB- denotes )
T4736 429-431 IN denotes at
T4737 432-434 CD denotes 42
T4738 434-436 NN denotes °C
T4739 437-440 IN denotes for
T4740 441-443 CD denotes 15
T4742 443-444 HYPH denotes -
T4741 444-447 NN denotes min
T4743 447-449 , denotes ,
T4744 449-452 CC denotes and
T4745 453-457 RB denotes then
T4746 458-464 VBD denotes washed
T4747 465-470 RB denotes twice
T4748 471-475 IN denotes with
T4749 476-483 NN denotes washing
T4750 484-490 NN denotes buffer
T4751 491-493 CD denotes II
T4752 494-495 -LRB- denotes (
T4754 495-499 CD denotes 0.25
T4756 499-500 SYM denotes ×
T4755 500-503 NN denotes SSC
T4757 503-505 , denotes ,
T4758 505-508 CD denotes 0.1
T4759 508-509 NN denotes %
T4753 510-513 NN denotes SDS
T4760 513-514 -RRB- denotes )
T4761 515-517 IN denotes at
T4762 518-520 CD denotes 65
T4763 520-522 NN denotes °C
T4764 523-526 IN denotes for
T4765 527-529 CD denotes 15
T4767 529-530 HYPH denotes -
T4766 530-533 NN denotes min
T4768 533-534 . denotes .
T4769 534-608 sentence denotes Washed filters were exposed to X-ray films for overnight or longer [3,4].
T4770 535-541 JJ denotes Washed
T4771 542-549 NNS denotes filters
T4773 550-554 VBD denotes were
T4772 555-562 VBN denotes exposed
T4774 563-565 IN denotes to
T4775 566-571 NN denotes X-ray
T4776 572-577 NNS denotes films
T4777 578-581 IN denotes for
T4778 582-591 NN denotes overnight
T4779 592-594 CC denotes or
T4780 595-601 RBR denotes longer
T4781 602-603 -LRB- denotes [
T4783 603-604 CD denotes 3
T4784 604-605 , denotes ,
T4782 605-606 CD denotes 4
T4785 606-607 -RRB- denotes ]
T4786 607-608 . denotes .
R2776 T4651 T4652 amod Multiple,filters
R2777 T4652 T4656 nsubjpass filters,purchased
R2778 T4653 T4652 compound Choice,filters
R2779 T4654 T4655 compound Northern,Blot
R2780 T4655 T4652 compound Blot,filters
R2781 T4657 T4652 acl containing,filters
R2782 T4658 T4659 nummod 12,tissues
R2783 T4659 T4657 dobj tissues,containing
R2784 T4660 T4659 amod different,tissues
R2785 T4661 T4659 compound mouse,tissues
R2786 T4662 T4656 auxpass were,purchased
R2787 T4663 T4656 prep from,purchased
R2788 T4664 T4663 pobj Origene,from
R2789 T4665 T4656 punct .,purchased
R2790 T4667 T4668 det The,filters
R2791 T4668 T4669 nsubjpass filters,probed
R2792 T4670 T4669 auxpass were,probed
R2793 T4671 T4669 prep for,probed
R2794 T4672 T4673 det each,gene
R2795 T4673 T4671 pobj gene,for
R2796 T4674 T4673 compound Acdp,gene
R2797 T4675 T4669 prep with,probed
R2798 T4676 T4677 det a,fragment
R2799 T4677 T4675 pobj fragment,with
R2800 T4678 T4677 compound PCR,fragment
R2801 T4679 T4680 punct (,bp
R2802 T4680 T4677 parataxis bp,fragment
R2803 T4681 T4682 quantmod around,350
R2804 T4682 T4680 nummod 350,bp
R2805 T4683 T4680 punct ),bp
R2806 T4684 T4677 prep from,fragment
R2807 T4685 T4686 amod last,exon
R2808 T4686 T4684 pobj exon,from
R2809 T4687 T4669 cc and,probed
R2810 T4688 T4689 det the,3
R2811 T4689 T4690 nummod 3,region
R2812 T4690 T4693 nsubj region,labeled
R2813 T4691 T4689 punct ',3
R2814 T4692 T4689 amod untranslated,3
R2815 T4693 T4669 conj labeled,probed
R2816 T4694 T4693 prep with,labeled
R2817 T4695 T4696 compound α,32P
R2818 T4696 T4698 compound 32P,dCTP
R2819 T4697 T4696 punct -,32P
R2820 T4698 T4694 pobj dCTP,with
R2821 T4699 T4693 advcl using,labeled
R2822 T4700 T4701 det the,system
R2823 T4701 T4699 dobj system,using
R2824 T4702 T4701 amod random,system
R2825 T4703 T4701 compound primer,system
R2826 T4704 T4701 compound extension,system
R2827 T4705 T4706 punct (,Technologies
R2828 T4706 T4701 parataxis Technologies,system
R2829 T4707 T4706 compound Life,Technologies
R2830 T4708 T4706 punct ),Technologies
R2831 T4709 T4693 punct .,labeled
R2832 T4711 T4712 nsubjpass Hybridizations,carried
R2833 T4713 T4712 auxpass were,carried
R2834 T4714 T4712 prt out,carried
R2835 T4715 T4712 advmod overnight,carried
R2836 T4716 T4712 punct .,carried
R2837 T4718 T4719 det The,filters
R2838 T4719 T4720 nsubjpass filters,washed
R2839 T4721 T4720 auxpass were,washed
R2840 T4722 T4720 advmod twice,washed
R2841 T4723 T4720 prep with,washed
R2842 T4724 T4725 amod washing,buffer
R2843 T4725 T4723 pobj buffer,with
R2844 T4726 T4725 nummod I,buffer
R2845 T4727 T4728 punct (,SDS
R2846 T4728 T4725 parataxis SDS,buffer
R2847 T4729 T4730 nummod 2,SSC
R2848 T4730 T4728 dep SSC,SDS
R2849 T4731 T4730 punct ×,SSC
R2850 T4732 T4728 punct ", ",SDS
R2851 T4733 T4734 nummod 0.1,%
R2852 T4734 T4728 compound %,SDS
R2853 T4735 T4728 punct ),SDS
R2854 T4736 T4720 prep at,washed
R2855 T4737 T4738 nummod 42,°C
R2856 T4738 T4736 pobj °C,at
R2857 T4739 T4720 prep for,washed
R2858 T4740 T4741 nummod 15,min
R2859 T4741 T4739 pobj min,for
R2860 T4742 T4741 punct -,min
R2861 T4743 T4720 punct ", ",washed
R2862 T4744 T4720 cc and,washed
R2863 T4745 T4746 advmod then,washed
R2864 T4746 T4720 conj washed,washed
R2865 T4747 T4746 advmod twice,washed
R2866 T4748 T4746 prep with,washed
R2867 T4749 T4750 compound washing,buffer
R2868 T4750 T4748 pobj buffer,with
R2869 T4751 T4750 nummod II,buffer
R2870 T4752 T4753 punct (,SDS
R2871 T4753 T4750 parataxis SDS,buffer
R2872 T4754 T4755 nummod 0.25,SSC
R2873 T4755 T4753 dep SSC,SDS
R2874 T4756 T4755 punct ×,SSC
R2875 T4757 T4753 punct ", ",SDS
R2876 T4758 T4759 nummod 0.1,%
R2877 T4759 T4753 compound %,SDS
R2878 T4760 T4753 punct ),SDS
R2879 T4761 T4746 prep at,washed
R2880 T4762 T4763 nummod 65,°C
R2881 T4763 T4761 pobj °C,at
R2882 T4764 T4746 prep for,washed
R2883 T4765 T4766 nummod 15,min
R2884 T4766 T4764 pobj min,for
R2885 T4767 T4766 punct -,min
R2886 T4768 T4720 punct .,washed
R2887 T4770 T4771 amod Washed,filters
R2888 T4771 T4772 nsubjpass filters,exposed
R2889 T4773 T4772 auxpass were,exposed
R2890 T4774 T4772 prep to,exposed
R2891 T4775 T4776 compound X-ray,films
R2892 T4776 T4774 pobj films,to
R2893 T4777 T4772 prep for,exposed
R2894 T4778 T4777 pcomp overnight,for
R2895 T4779 T4778 cc or,overnight
R2896 T4780 T4778 conj longer,overnight
R2897 T4781 T4782 punct [,4
R2898 T4782 T4772 parataxis 4,exposed
R2899 T4783 T4782 nummod 3,4
R2900 T4784 T4782 punct ",",4
R2901 T4785 T4782 punct ],4
R2902 T4786 T4772 punct .,exposed