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PMC:1564426 / 6613-8199 JSONTXT 4 Projects

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Id Subject Object Predicate Lexical cue
T4605 0-2 TO denotes To
T4606 3-14 VB denotes investigate
T4608 15-18 DT denotes the
T4609 19-23 NN denotes role
T4610 24-26 IN denotes of
T4611 27-30 NN denotes Apc
T4612 31-33 IN denotes in
T4613 34-45 NN denotes development
T4614 46-48 IN denotes of
T4615 49-53 NN denotes skin
T4616 54-57 CC denotes and
T4617 58-61 PRP$ denotes its
T4618 62-72 NNS denotes appendages
T4619 72-74 , denotes ,
T4620 74-76 PRP denotes we
T4607 77-81 VBD denotes used
T4621 82-85 DT denotes the
T4623 86-89 NN denotes Cre
T4625 89-90 HYPH denotes /
T4624 90-94 NN denotes loxP
T4622 95-105 NN denotes technology
T4626 106-108 TO denotes to
T4627 109-118 VB denotes introduce
T4628 119-120 DT denotes a
T4630 121-132 JJ denotes conditional
T4629 133-141 NN denotes mutation
T4631 142-144 IN denotes of
T4632 145-148 DT denotes the
T4634 149-152 NN denotes Apc
T4633 153-157 NN denotes gene
T4635 158-160 IN denotes in
T4636 161-165 NNS denotes mice
T4637 165-166 . denotes .
T4638 166-466 sentence denotes We constructed embryonic stem (ES) cells and mice carrying an Apc allele harboring both a pair of loxP sites flanking Apc exon 14 and a pair of FLP recognition target (FRT) sites flanking PGK-neomycin selection cassette by recombineering [12,13] (Figure 1A, ApcCKON allele, N for neomycin cassette).
T4639 167-169 PRP denotes We
T4640 170-181 VBD denotes constructed
T4641 182-191 JJ denotes embryonic
T4642 192-196 NN denotes stem
T4644 197-198 -LRB- denotes (
T4645 198-200 NN denotes ES
T4646 200-201 -RRB- denotes )
T4643 202-207 NNS denotes cells
T4647 208-211 CC denotes and
T4648 212-216 NNS denotes mice
T4649 217-225 VBG denotes carrying
T4650 226-228 DT denotes an
T4652 229-232 NN denotes Apc
T4651 233-239 NN denotes allele
T4653 240-249 VBG denotes harboring
T4654 250-254 CC denotes both
T4656 255-256 DT denotes a
T4655 257-261 NN denotes pair
T4657 262-264 IN denotes of
T4658 265-269 NN denotes loxP
T4659 270-275 NNS denotes sites
T4660 276-284 VBG denotes flanking
T4661 285-288 NN denotes Apc
T4662 289-293 NN denotes exon
T4663 294-296 CD denotes 14
T4664 297-300 CC denotes and
T4665 301-302 DT denotes a
T4666 303-307 NN denotes pair
T4667 308-310 IN denotes of
T4668 311-314 NN denotes FLP
T4670 315-326 NN denotes recognition
T4669 327-333 NN denotes target
T4672 334-335 -LRB- denotes (
T4673 335-338 NN denotes FRT
T4674 338-339 -RRB- denotes )
T4671 340-345 NNS denotes sites
T4675 346-354 VBG denotes flanking
T4676 355-358 NN denotes PGK
T4678 358-359 HYPH denotes -
T4677 359-367 NN denotes neomycin
T4680 368-377 NN denotes selection
T4679 378-386 NN denotes cassette
T4681 387-389 IN denotes by
T4682 390-404 VBG denotes recombineering
T4683 405-406 -LRB- denotes [
T4685 406-408 CD denotes 12
T4686 408-409 , denotes ,
T4684 409-411 CD denotes 13
T4687 411-412 -RRB- denotes ]
T4688 413-414 -LRB- denotes (
T4690 414-420 NN denotes Figure
T4691 421-423 NN denotes 1A
T4692 423-425 , denotes ,
T4693 425-432 NN denotes ApcCKON
T4694 433-439 NN denotes allele
T4695 439-441 , denotes ,
T4689 441-442 NN denotes N
T4696 443-446 IN denotes for
T4697 447-455 NN denotes neomycin
T4698 456-464 NN denotes cassette
T4699 464-465 -RRB- denotes )
T4700 465-466 . denotes .
T4701 466-587 sentence denotes A PGK-neomycin cassette was inserted in the same transcriptional orientation as Apc in intron 14 of the endogenous gene.
T4702 467-468 DT denotes A
T4704 469-472 NN denotes PGK
T4706 472-473 HYPH denotes -
T4705 473-481 NN denotes neomycin
T4703 482-490 NN denotes cassette
T4708 491-494 VBD denotes was
T4707 495-503 VBN denotes inserted
T4709 504-506 IN denotes in
T4710 507-510 DT denotes the
T4712 511-515 JJ denotes same
T4713 516-531 JJ denotes transcriptional
T4711 532-543 NN denotes orientation
T4714 544-546 IN denotes as
T4715 547-550 NN denotes Apc
T4716 551-553 IN denotes in
T4717 554-560 NN denotes intron
T4718 561-563 CD denotes 14
T4719 564-566 IN denotes of
T4720 567-570 DT denotes the
T4722 571-581 JJ denotes endogenous
T4721 582-586 NN denotes gene
T4723 586-587 . denotes .
T4724 587-665 sentence denotes The loxP and FRT sites were used to aid unidirectional recombination [12,13].
T4725 588-591 DT denotes The
T4727 592-596 NN denotes loxP
T4728 597-600 CC denotes and
T4729 601-604 NN denotes FRT
T4726 605-610 NNS denotes sites
T4731 611-615 VBD denotes were
T4730 616-620 VBN denotes used
T4732 621-623 TO denotes to
T4733 624-627 VB denotes aid
T4734 628-642 JJ denotes unidirectional
T4735 643-656 NN denotes recombination
T4736 657-658 -LRB- denotes [
T4738 658-660 CD denotes 12
T4739 660-661 , denotes ,
T4737 661-663 CD denotes 13
T4740 663-664 -RRB- denotes ]
T4741 664-665 . denotes .
T4742 665-815 sentence denotes Two mouse lines containing the same modification were generated from two independent ES clones to ensure that these two lines behave in the same way.
T4743 666-669 CD denotes Two
T4745 670-675 NN denotes mouse
T4744 676-681 NNS denotes lines
T4747 682-692 VBG denotes containing
T4748 693-696 DT denotes the
T4750 697-701 JJ denotes same
T4749 702-714 NN denotes modification
T4751 715-719 VBD denotes were
T4746 720-729 VBN denotes generated
T4752 730-734 IN denotes from
T4753 735-738 CD denotes two
T4755 739-750 JJ denotes independent
T4756 751-753 NN denotes ES
T4754 754-760 NNS denotes clones
T4757 761-763 TO denotes to
T4758 764-770 VB denotes ensure
T4759 771-775 IN denotes that
T4761 776-781 DT denotes these
T4763 782-785 CD denotes two
T4762 786-791 NNS denotes lines
T4760 792-798 VBP denotes behave
T4764 799-801 IN denotes in
T4765 802-805 DT denotes the
T4767 806-810 JJ denotes same
T4766 811-814 NN denotes way
T4768 814-815 . denotes .
T4769 815-1067 sentence denotes These ApcCKON/+ mice were crossed with FLPe-deleter to generate ApcCKO/+ mice that were heterozygous for the final Apc conditional (ApcCKO) allele that removed the PGK-neomycin cassette and contains only the loxP sites in the introns flanking exon 14.
T4770 816-821 DT denotes These
T4772 822-829 NN denotes ApcCKON
T4773 829-830 HYPH denotes /
T4774 830-831 SYM denotes +
T4771 832-836 NNS denotes mice
T4776 837-841 VBD denotes were
T4775 842-849 VBN denotes crossed
T4777 850-854 IN denotes with
T4778 855-859 NN denotes FLPe
T4780 859-860 HYPH denotes -
T4779 860-867 NN denotes deleter
T4781 868-870 TO denotes to
T4782 871-879 VB denotes generate
T4783 880-886 NN denotes ApcCKO
T4785 886-887 HYPH denotes /
T4786 887-888 SYM denotes +
T4784 889-893 NNS denotes mice
T4787 894-898 WDT denotes that
T4788 899-903 VBD denotes were
T4789 904-916 JJ denotes heterozygous
T4790 917-920 IN denotes for
T4791 921-924 DT denotes the
T4793 925-930 JJ denotes final
T4794 931-934 NN denotes Apc
T4795 935-946 JJ denotes conditional
T4796 947-948 -LRB- denotes (
T4797 948-954 NN denotes ApcCKO
T4798 954-955 -RRB- denotes )
T4792 956-962 NN denotes allele
T4799 963-967 WDT denotes that
T4800 968-975 VBD denotes removed
T4801 976-979 DT denotes the
T4803 980-983 NN denotes PGK
T4805 983-984 HYPH denotes -
T4804 984-992 NN denotes neomycin
T4802 993-1001 NN denotes cassette
T4806 1002-1005 CC denotes and
T4807 1006-1014 VBZ denotes contains
T4808 1015-1019 RB denotes only
T4810 1020-1023 DT denotes the
T4811 1024-1028 NN denotes loxP
T4809 1029-1034 NNS denotes sites
T4812 1035-1037 IN denotes in
T4813 1038-1041 DT denotes the
T4814 1042-1049 NNS denotes introns
T4815 1050-1058 VBG denotes flanking
T4816 1059-1063 NN denotes exon
T4817 1064-1066 CD denotes 14
T4818 1066-1067 . denotes .
T4819 1067-1248 sentence denotes To assess the effect of deleting exon 14 in mice, both lines of ApcCKO/+ mice were crossed with the Cre-deleter to generate the germline knockout line of Apc, designated ApcΔ580/+.
T4820 1068-1070 TO denotes To
T4821 1071-1077 VB denotes assess
T4823 1078-1081 DT denotes the
T4824 1082-1088 NN denotes effect
T4825 1089-1091 IN denotes of
T4826 1092-1100 VBG denotes deleting
T4827 1101-1105 NN denotes exon
T4828 1106-1108 CD denotes 14
T4829 1109-1111 IN denotes in
T4830 1112-1116 NNS denotes mice
T4831 1116-1118 , denotes ,
T4832 1118-1122 DT denotes both
T4833 1123-1128 NNS denotes lines
T4834 1129-1131 IN denotes of
T4835 1132-1138 NN denotes ApcCKO
T4837 1138-1139 HYPH denotes /
T4838 1139-1140 SYM denotes +
T4836 1141-1145 NNS denotes mice
T4839 1146-1150 VBD denotes were
T4822 1151-1158 VBN denotes crossed
T4840 1159-1163 IN denotes with
T4841 1164-1167 DT denotes the
T4843 1168-1171 NN denotes Cre
T4844 1171-1172 HYPH denotes -
T4842 1172-1179 NN denotes deleter
T4845 1180-1182 TO denotes to
T4846 1183-1191 VB denotes generate
T4847 1192-1195 DT denotes the
T4849 1196-1204 NN denotes germline
T4850 1205-1213 NN denotes knockout
T4848 1214-1218 NN denotes line
T4851 1219-1221 IN denotes of
T4852 1222-1225 NNP denotes Apc
T4853 1225-1227 , denotes ,
T4854 1227-1237 VBN denotes designated
T4855 1238-1245 NN denotes ApcΔ580
T4856 1245-1246 HYPH denotes /
T4857 1246-1247 SYM denotes +
T4858 1247-1248 . denotes .
T4859 1248-1303 sentence denotes The mutant allele (ApcΔ580) lacks exon 14 (Figure 1A).
T4860 1249-1252 DT denotes The
T4862 1253-1259 JJ denotes mutant
T4861 1260-1266 NN denotes allele
T4864 1267-1268 -LRB- denotes (
T4865 1268-1275 NN denotes ApcΔ580
T4866 1275-1276 -RRB- denotes )
T4863 1277-1282 VBZ denotes lacks
T4867 1283-1287 NN denotes exon
T4868 1288-1290 CD denotes 14
T4869 1291-1292 -LRB- denotes (
T4871 1292-1298 NN denotes Figure
T4870 1299-1301 NN denotes 1A
T4872 1301-1302 -RRB- denotes )
T4873 1302-1303 . denotes .
T4874 1303-1586 sentence denotes The transcript from loss of exon 14 results in a shift in the normal reading frame, resulting in a premature chain termination codon which, if utilized, would result in a truncated polypeptide that is 605 aa in length, of which the first 580 aa correspond to the normal Apc protein.
T4875 1304-1307 DT denotes The
T4876 1308-1318 NN denotes transcript
T4878 1319-1323 IN denotes from
T4879 1324-1328 NN denotes loss
T4880 1329-1331 IN denotes of
T4881 1332-1336 NN denotes exon
T4882 1337-1339 CD denotes 14
T4877 1340-1347 VBZ denotes results
T4883 1348-1350 IN denotes in
T4884 1351-1352 DT denotes a
T4885 1353-1358 NN denotes shift
T4886 1359-1361 IN denotes in
T4887 1362-1365 DT denotes the
T4889 1366-1372 JJ denotes normal
T4890 1373-1380 NN denotes reading
T4888 1381-1386 NN denotes frame
T4891 1386-1388 , denotes ,
T4892 1388-1397 VBG denotes resulting
T4893 1398-1400 IN denotes in
T4894 1401-1402 DT denotes a
T4896 1403-1412 JJ denotes premature
T4897 1413-1418 NN denotes chain
T4898 1419-1430 NN denotes termination
T4895 1431-1436 NN denotes codon
T4899 1437-1442 WDT denotes which
T4901 1442-1444 , denotes ,
T4902 1444-1446 IN denotes if
T4903 1447-1455 VBN denotes utilized
T4904 1455-1457 , denotes ,
T4905 1457-1462 MD denotes would
T4900 1463-1469 VB denotes result
T4906 1470-1472 IN denotes in
T4907 1473-1474 DT denotes a
T4909 1475-1484 VBN denotes truncated
T4908 1485-1496 NN denotes polypeptide
T4910 1497-1501 WDT denotes that
T4911 1502-1504 VBZ denotes is
T4912 1505-1508 CD denotes 605
T4913 1509-1511 NNS denotes aa
T4914 1512-1514 IN denotes in
T4915 1515-1521 NN denotes length
T4916 1521-1523 , denotes ,
T4917 1523-1525 IN denotes of
T4919 1526-1531 WDT denotes which
T4920 1532-1535 DT denotes the
T4922 1536-1541 JJ denotes first
T4923 1542-1545 CD denotes 580
T4921 1546-1548 NNS denotes aa
T4918 1549-1559 VBP denotes correspond
T4924 1560-1562 IN denotes to
T4925 1563-1566 DT denotes the
T4927 1567-1573 JJ denotes normal
T4928 1574-1577 NN denotes Apc
T4926 1578-1585 NN denotes protein
T4929 1585-1586 . denotes .
R1000 T4684 T4640 parataxis 13,constructed
R1001 T4685 T4684 nummod 12,13
R1002 T4686 T4684 punct ",",13
R1003 T4687 T4684 punct ],13
R1004 T4688 T4689 punct (,N
R1005 T4689 T4640 parataxis N,constructed
R1006 T4690 T4691 compound Figure,1A
R1007 T4691 T4689 dep 1A,N
R1008 T4692 T4689 punct ", ",N
R1009 T4693 T4694 compound ApcCKON,allele
R1010 T4694 T4689 dep allele,N
R1011 T4695 T4689 punct ", ",N
R1012 T4696 T4689 prep for,N
R1013 T4697 T4698 compound neomycin,cassette
R1014 T4698 T4696 pobj cassette,for
R1015 T4699 T4689 punct ),N
R1016 T4700 T4640 punct .,constructed
R1017 T4702 T4703 det A,cassette
R1018 T4703 T4707 nsubjpass cassette,inserted
R1019 T4704 T4705 compound PGK,neomycin
R1020 T4705 T4703 compound neomycin,cassette
R1021 T4706 T4705 punct -,neomycin
R1022 T4708 T4707 auxpass was,inserted
R1023 T4709 T4707 prep in,inserted
R1024 T4710 T4711 det the,orientation
R1025 T4711 T4709 pobj orientation,in
R1026 T4712 T4711 amod same,orientation
R1027 T4713 T4711 amod transcriptional,orientation
R1028 T4714 T4711 prep as,orientation
R1029 T4715 T4714 pobj Apc,as
R1030 T4716 T4707 prep in,inserted
R1031 T4717 T4716 pobj intron,in
R1032 T4718 T4717 nummod 14,intron
R1033 T4719 T4717 prep of,intron
R1034 T4720 T4721 det the,gene
R1035 T4721 T4719 pobj gene,of
R1036 T4722 T4721 amod endogenous,gene
R1037 T4723 T4707 punct .,inserted
R1038 T4725 T4726 det The,sites
R1039 T4726 T4730 nsubjpass sites,used
R1040 T4727 T4726 nmod loxP,sites
R1041 T4728 T4727 cc and,loxP
R1042 T4729 T4727 conj FRT,loxP
R1043 T4731 T4730 auxpass were,used
R1044 T4732 T4733 aux to,aid
R1045 T4733 T4730 advcl aid,used
R1046 T4734 T4735 amod unidirectional,recombination
R1047 T4735 T4733 dobj recombination,aid
R1048 T4736 T4737 punct [,13
R1049 T4737 T4730 parataxis 13,used
R1050 T4738 T4737 nummod 12,13
R1051 T4739 T4737 punct ",",13
R1052 T4740 T4737 punct ],13
R1053 T4741 T4730 punct .,used
R1054 T4743 T4744 nummod Two,lines
R1055 T4744 T4746 nsubjpass lines,generated
R1056 T4745 T4744 compound mouse,lines
R1057 T4747 T4744 acl containing,lines
R1058 T4748 T4749 det the,modification
R1059 T4749 T4747 dobj modification,containing
R1060 T4750 T4749 amod same,modification
R1061 T4751 T4746 auxpass were,generated
R1062 T4752 T4746 prep from,generated
R1063 T4753 T4754 nummod two,clones
R1064 T4754 T4752 pobj clones,from
R1065 T4755 T4754 amod independent,clones
R1066 T4756 T4754 compound ES,clones
R1067 T4757 T4758 aux to,ensure
R1068 T4758 T4746 advcl ensure,generated
R1069 T4759 T4760 mark that,behave
R1070 T4760 T4758 ccomp behave,ensure
R1071 T4761 T4762 det these,lines
R1072 T4762 T4760 nsubj lines,behave
R1073 T4763 T4762 nummod two,lines
R1074 T4764 T4760 prep in,behave
R1075 T4765 T4766 det the,way
R1076 T4766 T4764 pobj way,in
R1077 T4767 T4766 amod same,way
R1078 T4768 T4746 punct .,generated
R1079 T4770 T4771 det These,mice
R1080 T4771 T4775 nsubjpass mice,crossed
R1081 T4772 T4771 nmod ApcCKON,mice
R1082 T4773 T4772 punct /,ApcCKON
R1083 T4774 T4772 punct +,ApcCKON
R1084 T4776 T4775 auxpass were,crossed
R1085 T4777 T4775 prep with,crossed
R1086 T4778 T4779 compound FLPe,deleter
R1087 T4779 T4777 pobj deleter,with
R1088 T4780 T4779 punct -,deleter
R1089 T4781 T4782 aux to,generate
R1090 T4782 T4775 advcl generate,crossed
R1091 T4783 T4784 nmod ApcCKO,mice
R1092 T4784 T4782 dobj mice,generate
R1093 T4785 T4783 punct /,ApcCKO
R1094 T4786 T4783 punct +,ApcCKO
R1095 T4787 T4788 dep that,were
R1096 T4788 T4784 relcl were,mice
R1097 T4789 T4788 acomp heterozygous,were
R1098 T4790 T4789 prep for,heterozygous
R1099 T4791 T4792 det the,allele
R1100 T4792 T4790 pobj allele,for
R1101 T4793 T4792 amod final,allele
R1102 T4794 T4795 npadvmod Apc,conditional
R1103 T4795 T4792 amod conditional,allele
R1104 T4796 T4797 punct (,ApcCKO
R1105 T4797 T4795 parataxis ApcCKO,conditional
R1106 T4798 T4797 punct ),ApcCKO
R1107 T4799 T4800 dep that,removed
R1108 T4800 T4792 relcl removed,allele
R1109 T4801 T4802 det the,cassette
R1110 T4802 T4800 dobj cassette,removed
R1111 T4803 T4804 compound PGK,neomycin
R1112 T4804 T4802 compound neomycin,cassette
R1113 T4805 T4804 punct -,neomycin
R1114 T4806 T4800 cc and,removed
R1115 T4807 T4800 conj contains,removed
R1116 T4808 T4809 advmod only,sites
R1117 T4809 T4807 dobj sites,contains
R1118 T4810 T4809 det the,sites
R1119 T4811 T4809 compound loxP,sites
R1120 T4812 T4807 prep in,contains
R1121 T4813 T4814 det the,introns
R1122 T4814 T4812 pobj introns,in
R1123 T4815 T4814 acl flanking,introns
R1124 T4816 T4815 dobj exon,flanking
R1125 T4817 T4816 nummod 14,exon
R1126 T4818 T4775 punct .,crossed
R1127 T4820 T4821 aux To,assess
R1128 T4821 T4822 advcl assess,crossed
R1129 T4823 T4824 det the,effect
R1130 T4824 T4821 dobj effect,assess
R1131 T4825 T4824 prep of,effect
R1132 T4826 T4825 pcomp deleting,of
R1133 T4827 T4826 dobj exon,deleting
R1134 T4828 T4827 nummod 14,exon
R1135 T4829 T4821 prep in,assess
R1136 T4830 T4829 pobj mice,in
R1137 T4831 T4822 punct ", ",crossed
R1138 T4832 T4833 det both,lines
R1139 T4833 T4822 nsubjpass lines,crossed
R1140 T4834 T4833 prep of,lines
R1141 T4835 T4836 nmod ApcCKO,mice
R1142 T4836 T4834 pobj mice,of
R1143 T4837 T4835 punct /,ApcCKO
R1144 T4838 T4835 punct +,ApcCKO
R1145 T4839 T4822 auxpass were,crossed
R1146 T4840 T4822 prep with,crossed
R1147 T4841 T4842 det the,deleter
R1148 T4842 T4840 pobj deleter,with
R1149 T4843 T4842 compound Cre,deleter
R1150 T4844 T4842 punct -,deleter
R1151 T4845 T4846 aux to,generate
R1152 T4846 T4822 advcl generate,crossed
R1153 T4847 T4848 det the,line
R1154 T4848 T4846 dobj line,generate
R1155 T4849 T4850 compound germline,knockout
R1156 T4850 T4848 compound knockout,line
R1157 T4851 T4848 prep of,line
R1158 T4852 T4851 pobj Apc,of
R1159 T4853 T4848 punct ", ",line
R1160 T4854 T4848 acl designated,line
R1161 T4855 T4854 oprd ApcΔ580,designated
R1162 T4856 T4855 punct /,ApcΔ580
R1163 T4857 T4855 punct +,ApcΔ580
R1164 T4858 T4822 punct .,crossed
R1165 T4860 T4861 det The,allele
R1166 T4861 T4863 nsubj allele,lacks
R1167 T4862 T4861 amod mutant,allele
R1168 T4864 T4861 punct (,allele
R1169 T4865 T4861 appos ApcΔ580,allele
R1170 T4866 T4863 punct ),lacks
R1171 T4867 T4863 dobj exon,lacks
R1172 T4868 T4867 nummod 14,exon
R1173 T4869 T4870 punct (,1A
R1174 T4870 T4863 parataxis 1A,lacks
R1175 T4871 T4870 compound Figure,1A
R1176 T4872 T4870 punct ),1A
R1177 T4873 T4863 punct .,lacks
R1178 T4875 T4876 det The,transcript
R1179 T4876 T4877 nsubj transcript,results
R1180 T4878 T4876 prep from,transcript
R1181 T4879 T4878 pobj loss,from
R1182 T4880 T4879 prep of,loss
R1183 T4881 T4880 pobj exon,of
R1184 T4882 T4881 nummod 14,exon
R1185 T4883 T4877 prep in,results
R1186 T4884 T4885 det a,shift
R1187 T4885 T4883 pobj shift,in
R1188 T4886 T4885 prep in,shift
R1189 T4887 T4888 det the,frame
R1190 T4888 T4886 pobj frame,in
R1191 T4889 T4888 amod normal,frame
R1192 T4890 T4888 compound reading,frame
R1193 T4891 T4877 punct ", ",results
R1194 T4892 T4877 advcl resulting,results
R1195 T4893 T4892 prep in,resulting
R1196 T4894 T4895 det a,codon
R1197 T4895 T4893 pobj codon,in
R1198 T4896 T4895 amod premature,codon
R1199 T4897 T4898 compound chain,termination
R1200 T4898 T4895 compound termination,codon
R1201 T4899 T4900 dep which,result
R1202 T4900 T4895 relcl result,codon
R1203 T4901 T4900 punct ", ",result
R1204 T4902 T4903 mark if,utilized
R1205 T4903 T4900 advcl utilized,result
R1206 T4904 T4900 punct ", ",result
R1207 T4905 T4900 aux would,result
R1208 T4906 T4900 prep in,result
R1209 T4907 T4908 det a,polypeptide
R1210 T4908 T4906 pobj polypeptide,in
R1211 T4909 T4908 amod truncated,polypeptide
R1212 T4910 T4911 dep that,is
R1213 T4911 T4908 relcl is,polypeptide
R1214 T4912 T4913 nummod 605,aa
R1215 T4913 T4911 attr aa,is
R1216 T4914 T4913 prep in,aa
R1217 T4915 T4914 pobj length,in
R1218 T4916 T4913 punct ", ",aa
R1219 T4917 T4918 prep of,correspond
R1220 T4918 T4913 relcl correspond,aa
R1221 T4919 T4917 pobj which,of
R1222 T4920 T4921 det the,aa
R1223 T4921 T4918 nsubj aa,correspond
R1224 T4922 T4921 amod first,aa
R1225 T4923 T4921 nummod 580,aa
R1226 T4924 T4918 prep to,correspond
R1227 T4925 T4926 det the,protein
R1228 T4926 T4924 pobj protein,to
R1229 T4927 T4926 amod normal,protein
R1230 T4928 T4926 compound Apc,protein
R1231 T4929 T4877 punct .,results
R924 T4605 T4606 aux To,investigate
R925 T4606 T4607 advcl investigate,used
R926 T4608 T4609 det the,role
R927 T4609 T4606 dobj role,investigate
R928 T4610 T4609 prep of,role
R929 T4611 T4610 pobj Apc,of
R930 T4612 T4609 prep in,role
R931 T4613 T4612 pobj development,in
R932 T4614 T4613 prep of,development
R933 T4615 T4614 pobj skin,of
R934 T4616 T4615 cc and,skin
R935 T4617 T4618 poss its,appendages
R936 T4618 T4615 conj appendages,skin
R937 T4619 T4607 punct ", ",used
R938 T4620 T4607 nsubj we,used
R939 T4621 T4622 det the,technology
R940 T4622 T4607 dobj technology,used
R941 T4623 T4624 compound Cre,loxP
R942 T4624 T4622 compound loxP,technology
R943 T4625 T4624 punct /,loxP
R944 T4626 T4627 aux to,introduce
R945 T4627 T4607 advcl introduce,used
R946 T4628 T4629 det a,mutation
R947 T4629 T4627 dobj mutation,introduce
R948 T4630 T4629 amod conditional,mutation
R949 T4631 T4629 prep of,mutation
R950 T4632 T4633 det the,gene
R951 T4633 T4631 pobj gene,of
R952 T4634 T4633 compound Apc,gene
R953 T4635 T4627 prep in,introduce
R954 T4636 T4635 pobj mice,in
R955 T4637 T4607 punct .,used
R956 T4639 T4640 nsubj We,constructed
R957 T4641 T4642 amod embryonic,stem
R958 T4642 T4643 nmod stem,cells
R959 T4643 T4640 dobj cells,constructed
R960 T4644 T4642 punct (,stem
R961 T4645 T4642 appos ES,stem
R962 T4646 T4643 punct ),cells
R963 T4647 T4643 cc and,cells
R964 T4648 T4643 conj mice,cells
R965 T4649 T4648 acl carrying,mice
R966 T4650 T4651 det an,allele
R967 T4651 T4649 dobj allele,carrying
R968 T4652 T4651 compound Apc,allele
R969 T4653 T4651 acl harboring,allele
R970 T4654 T4655 preconj both,pair
R971 T4655 T4653 dobj pair,harboring
R972 T4656 T4655 det a,pair
R973 T4657 T4655 prep of,pair
R974 T4658 T4659 compound loxP,sites
R975 T4659 T4657 pobj sites,of
R976 T4660 T4655 acl flanking,pair
R977 T4661 T4662 compound Apc,exon
R978 T4662 T4660 dobj exon,flanking
R979 T4663 T4662 nummod 14,exon
R980 T4664 T4655 cc and,pair
R981 T4665 T4666 det a,pair
R982 T4666 T4655 conj pair,pair
R983 T4667 T4666 prep of,pair
R984 T4668 T4669 nmod FLP,target
R985 T4669 T4671 nmod target,sites
R986 T4670 T4669 nmod recognition,target
R987 T4671 T4667 pobj sites,of
R988 T4672 T4669 punct (,target
R989 T4673 T4669 appos FRT,target
R990 T4674 T4671 punct ),sites
R991 T4675 T4666 acl flanking,pair
R992 T4676 T4677 compound PGK,neomycin
R993 T4677 T4679 compound neomycin,cassette
R994 T4678 T4677 punct -,neomycin
R995 T4679 T4675 dobj cassette,flanking
R996 T4680 T4679 compound selection,cassette
R997 T4681 T4640 prep by,constructed
R998 T4682 T4681 pobj recombineering,by
R999 T4683 T4684 punct [,13