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PMC:1564426 / 54546-55129 JSONTXT 4 Projects

Annnotations TAB TSV DIC JSON TextAE

Id Subject Object Predicate Lexical cue
T28760 0-4 NN denotes Skin
T28761 5-17 NN denotes permeability
T28762 18-23 NN denotes assay
T28763 23-24 . denotes .
T28764 24-253 sentence denotes Unfixed and freshly isolated E18.5 embryos were rinsed in PBS and then submerged in X-gal reaction mix at pH 4.5 (100 mM NaPO4, 1.3 mM MgCl2, 3 mM K3Fe(CN)6, 3 mM K4Fe(CN)6, and 1 mg/ml X-gal) at room temperature overnight [36].
T28765 25-32 JJ denotes Unfixed
T28767 33-36 CC denotes and
T28768 37-44 RB denotes freshly
T28769 45-53 VBN denotes isolated
T28770 54-59 NN denotes E18.5
T28766 60-67 NNS denotes embryos
T28772 68-72 VBD denotes were
T28771 73-79 VBN denotes rinsed
T28773 80-82 IN denotes in
T28774 83-86 NNS denotes PBS
T28775 87-90 CC denotes and
T28776 91-95 RB denotes then
T28777 96-105 VBD denotes submerged
T28778 106-108 IN denotes in
T28779 109-110 NN denotes X
T28781 110-111 HYPH denotes -
T28780 111-114 NN denotes gal
T28783 115-123 NN denotes reaction
T28782 124-127 NN denotes mix
T28784 128-130 IN denotes at
T28785 131-133 NN denotes pH
T28786 134-137 CD denotes 4.5
T28787 138-139 -LRB- denotes (
T28789 139-142 CD denotes 100
T28790 143-145 NNS denotes mM
T28788 146-151 NN denotes NaPO4
T28791 151-153 , denotes ,
T28792 153-156 CD denotes 1.3
T28793 157-159 NNS denotes mM
T28794 160-165 NN denotes MgCl2
T28795 165-167 , denotes ,
T28796 167-168 CD denotes 3
T28797 169-171 NN denotes mM
T28798 172-176 NN denotes K3Fe
T28799 176-177 -LRB- denotes (
T28800 177-179 NN denotes CN
T28801 179-180 -RRB- denotes )
T28802 180-181 CD denotes 6
T28803 181-183 , denotes ,
T28804 183-184 CD denotes 3
T28805 185-187 NN denotes mM
T28806 188-192 NN denotes K4Fe
T28807 192-193 -LRB- denotes (
T28808 193-195 NN denotes CN
T28809 195-196 -RRB- denotes )
T28810 196-197 CD denotes 6
T28811 197-199 , denotes ,
T28812 199-202 CC denotes and
T28813 203-204 CD denotes 1
T28814 205-207 NN denotes mg
T28816 207-208 SYM denotes /
T28817 208-210 NN denotes ml
T28818 211-212 NN denotes X
T28819 212-213 HYPH denotes -
T28815 213-216 NN denotes gal
T28820 216-217 -RRB- denotes )
T28821 218-220 IN denotes at
T28822 221-225 NN denotes room
T28823 226-237 NN denotes temperature
T28824 238-247 RB denotes overnight
T28825 248-249 -LRB- denotes [
T28826 249-251 CD denotes 36
T28827 251-252 -RRB- denotes ]
T28828 252-253 . denotes .
T28829 253-427 sentence denotes At this pH in the absence of epidermal barrier, the solution penetrates epidermis and an endogenous β-galactosidase–like activity catalyzes production of a blue precipitate.
T28830 254-256 IN denotes At
T28832 257-261 DT denotes this
T28833 262-264 NN denotes pH
T28834 265-267 IN denotes in
T28835 268-271 DT denotes the
T28836 272-279 NN denotes absence
T28837 280-282 IN denotes of
T28838 283-292 JJ denotes epidermal
T28839 293-300 NN denotes barrier
T28840 300-302 , denotes ,
T28841 302-305 DT denotes the
T28842 306-314 NN denotes solution
T28831 315-325 VBZ denotes penetrates
T28843 326-335 NN denotes epidermis
T28844 336-339 CC denotes and
T28845 340-342 DT denotes an
T28847 343-353 JJ denotes endogenous
T28848 354-355 JJ denotes β
T28850 355-356 HYPH denotes -
T28849 356-369 NN denotes galactosidase
T28852 369-370 HYPH denotes –
T28851 370-374 JJ denotes like
T28846 375-383 NN denotes activity
T28853 384-393 VBZ denotes catalyzes
T28854 394-404 NN denotes production
T28855 405-407 IN denotes of
T28856 408-409 DT denotes a
T28858 410-414 JJ denotes blue
T28857 415-426 NN denotes precipitate
T28859 426-427 . denotes .
T28860 427-583 sentence denotes After staining, embryos were washed twice in PBS, fixed overnight at 4 °C in 4% paraformaldehyde, and were photographed using a 35 mm Nikon digital camera.
T28861 428-433 IN denotes After
T28863 434-442 NN denotes staining
T28864 442-444 , denotes ,
T28865 444-451 NNS denotes embryos
T28866 452-456 VBD denotes were
T28862 457-463 VBN denotes washed
T28867 464-469 RB denotes twice
T28868 470-472 IN denotes in
T28869 473-476 NNP denotes PBS
T28870 476-478 , denotes ,
T28871 478-483 VBN denotes fixed
T28872 484-493 RB denotes overnight
T28873 494-496 IN denotes at
T28874 497-498 CD denotes 4
T28875 499-501 NNS denotes °C
T28876 502-504 IN denotes in
T28877 505-506 CD denotes 4
T28878 506-507 NN denotes %
T28879 508-524 NN denotes paraformaldehyde
T28880 524-526 , denotes ,
T28881 526-529 CC denotes and
T28882 530-534 VBD denotes were
T28883 535-547 VBN denotes photographed
T28884 548-553 VBG denotes using
T28885 554-555 DT denotes a
T28887 556-558 CD denotes 35
T28888 559-561 NN denotes mm
T28889 562-567 NNP denotes Nikon
T28890 568-575 JJ denotes digital
T28886 576-582 NN denotes camera
T28891 582-583 . denotes .
R8738 T28760 T28761 compound Skin,permeability
R8739 T28761 T28762 compound permeability,assay
R8740 T28763 T28762 punct .,assay
R8741 T28765 T28766 amod Unfixed,embryos
R8742 T28766 T28771 nsubjpass embryos,rinsed
R8743 T28767 T28765 cc and,Unfixed
R8744 T28768 T28769 advmod freshly,isolated
R8745 T28769 T28765 conj isolated,Unfixed
R8746 T28770 T28766 compound E18.5,embryos
R8747 T28772 T28771 auxpass were,rinsed
R8748 T28773 T28771 prep in,rinsed
R8749 T28774 T28773 pobj PBS,in
R8750 T28775 T28771 cc and,rinsed
R8751 T28776 T28777 advmod then,submerged
R8752 T28777 T28771 conj submerged,rinsed
R8753 T28778 T28777 prep in,submerged
R8754 T28779 T28780 compound X,gal
R8755 T28780 T28782 compound gal,mix
R8756 T28781 T28780 punct -,gal
R8757 T28782 T28778 pobj mix,in
R8758 T28783 T28782 compound reaction,mix
R8759 T28784 T28782 prep at,mix
R8760 T28785 T28784 pobj pH,at
R8761 T28786 T28785 nummod 4.5,pH
R8762 T28787 T28788 punct (,NaPO4
R8763 T28788 T28782 parataxis NaPO4,mix
R8764 T28789 T28790 nummod 100,mM
R8765 T28790 T28788 compound mM,NaPO4
R8766 T28791 T28788 punct ", ",NaPO4
R8767 T28792 T28793 nummod 1.3,mM
R8768 T28793 T28794 compound mM,MgCl2
R8769 T28794 T28788 conj MgCl2,NaPO4
R8770 T28795 T28794 punct ", ",MgCl2
R8771 T28796 T28797 nummod 3,mM
R8772 T28797 T28798 compound mM,K3Fe
R8773 T28798 T28794 conj K3Fe,MgCl2
R8774 T28799 T28800 punct (,CN
R8775 T28800 T28798 parataxis CN,K3Fe
R8776 T28801 T28800 punct ),CN
R8777 T28802 T28798 nummod 6,K3Fe
R8778 T28803 T28798 punct ", ",K3Fe
R8779 T28804 T28805 nummod 3,mM
R8780 T28805 T28806 compound mM,K4Fe
R8781 T28806 T28798 conj K4Fe,K3Fe
R8782 T28807 T28808 punct (,CN
R8783 T28808 T28806 parataxis CN,K4Fe
R8784 T28809 T28808 punct ),CN
R8785 T28810 T28806 nummod 6,K4Fe
R8786 T28811 T28806 punct ", ",K4Fe
R8787 T28812 T28806 cc and,K4Fe
R8788 T28813 T28814 nummod 1,mg
R8789 T28814 T28815 nmod mg,gal
R8790 T28815 T28806 conj gal,K4Fe
R8791 T28816 T28817 punct /,ml
R8792 T28817 T28814 prep ml,mg
R8793 T28818 T28815 compound X,gal
R8794 T28819 T28815 punct -,gal
R8795 T28820 T28815 punct ),gal
R8796 T28821 T28777 prep at,submerged
R8797 T28822 T28823 compound room,temperature
R8798 T28823 T28821 pobj temperature,at
R8799 T28824 T28777 advmod overnight,submerged
R8800 T28825 T28826 punct [,36
R8801 T28826 T28777 parataxis 36,submerged
R8802 T28827 T28826 punct ],36
R8803 T28828 T28771 punct .,rinsed
R8804 T28830 T28831 prep At,penetrates
R8805 T28832 T28833 det this,pH
R8806 T28833 T28830 pobj pH,At
R8807 T28834 T28831 prep in,penetrates
R8808 T28835 T28836 det the,absence
R8809 T28836 T28834 pobj absence,in
R8810 T28837 T28836 prep of,absence
R8811 T28838 T28839 amod epidermal,barrier
R8812 T28839 T28837 pobj barrier,of
R8813 T28840 T28831 punct ", ",penetrates
R8814 T28841 T28842 det the,solution
R8815 T28842 T28831 nsubj solution,penetrates
R8816 T28843 T28831 dobj epidermis,penetrates
R8817 T28844 T28831 cc and,penetrates
R8818 T28845 T28846 det an,activity
R8819 T28846 T28853 nsubj activity,catalyzes
R8820 T28847 T28846 amod endogenous,activity
R8821 T28848 T28849 amod β,galactosidase
R8822 T28849 T28851 npadvmod galactosidase,like
R8823 T28850 T28849 punct -,galactosidase
R8824 T28851 T28846 amod like,activity
R8825 T28852 T28851 punct –,like
R8826 T28853 T28831 conj catalyzes,penetrates
R8827 T28854 T28853 dobj production,catalyzes
R8828 T28855 T28854 prep of,production
R8829 T28856 T28857 det a,precipitate
R8830 T28857 T28855 pobj precipitate,of
R8831 T28858 T28857 amod blue,precipitate
R8832 T28859 T28831 punct .,penetrates
R8833 T28861 T28862 prep After,washed
R8834 T28863 T28861 pobj staining,After
R8835 T28864 T28862 punct ", ",washed
R8836 T28865 T28862 nsubjpass embryos,washed
R8837 T28866 T28862 auxpass were,washed
R8838 T28867 T28862 advmod twice,washed
R8839 T28868 T28862 prep in,washed
R8840 T28869 T28868 pobj PBS,in
R8841 T28870 T28862 punct ", ",washed
R8842 T28871 T28862 dep fixed,washed
R8843 T28872 T28871 advmod overnight,fixed
R8844 T28873 T28871 prep at,fixed
R8845 T28874 T28875 nummod 4,°C
R8846 T28875 T28873 pobj °C,at
R8847 T28876 T28871 prep in,fixed
R8848 T28877 T28878 nummod 4,%
R8849 T28878 T28879 compound %,paraformaldehyde
R8850 T28879 T28876 pobj paraformaldehyde,in
R8851 T28880 T28862 punct ", ",washed
R8852 T28881 T28862 cc and,washed
R8853 T28882 T28883 auxpass were,photographed
R8854 T28883 T28862 conj photographed,washed
R8855 T28884 T28883 advcl using,photographed
R8856 T28885 T28886 det a,camera
R8857 T28886 T28884 dobj camera,using
R8858 T28887 T28888 nummod 35,mm
R8859 T28888 T28886 nmod mm,camera
R8860 T28889 T28886 nmod Nikon,camera
R8861 T28890 T28886 amod digital,camera
R8862 T28891 T28862 punct .,washed