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PMC:1564426 / 18675-20927 JSONTXT 3 Projects

Annnotations TAB TSV DIC JSON TextAE

Id Subject Object Predicate Lexical cue
T9607 0-8 NN denotes Genotype
T9609 8-9 HYPH denotes -
T9610 10-13 CC denotes and
T9611 14-20 NN denotes Tissue
T9612 20-21 HYPH denotes -
T9608 21-29 JJ denotes Specific
T9613 30-40 NN denotes Expression
T9614 41-43 IN denotes of
T9615 44-47 DT denotes the
T9617 48-57 VBN denotes Truncated
T9618 58-61 NN denotes Apc
T9616 62-73 NNS denotes Transcripts
T9619 73-207 sentence denotes To assess the molecular effects of the K14-cre–mediated recombination, we screened for the presence of deleted Apc (ApcΔ580) alleles.
T9620 74-76 TO denotes To
T9621 77-83 VB denotes assess
T9623 84-87 DT denotes the
T9625 88-97 JJ denotes molecular
T9624 98-105 NNS denotes effects
T9626 106-108 IN denotes of
T9627 109-112 DT denotes the
T9629 113-116 NN denotes K14
T9631 116-117 HYPH denotes -
T9630 117-120 NN denotes cre
T9633 120-121 HYPH denotes –
T9632 121-129 JJ denotes mediated
T9628 130-143 NN denotes recombination
T9634 143-145 , denotes ,
T9635 145-147 PRP denotes we
T9622 148-156 VBD denotes screened
T9636 157-160 IN denotes for
T9637 161-164 DT denotes the
T9638 165-173 NN denotes presence
T9639 174-176 IN denotes of
T9640 177-184 VBN denotes deleted
T9641 185-188 NN denotes Apc
T9643 189-190 -LRB- denotes (
T9644 190-197 NN denotes ApcΔ580
T9645 197-198 -RRB- denotes )
T9642 199-206 NNS denotes alleles
T9646 206-207 . denotes .
T9647 207-359 sentence denotes Genomic DNA was extracted from liver, thymus, and skin from all 4 possible genotypes: K14-cre; ApcCKO/CKO, K14-cre; ApcCKO/+, ApcCKO/CKO, and ApcCKO/+.
T9648 208-215 JJ denotes Genomic
T9649 216-219 NN denotes DNA
T9651 220-223 VBD denotes was
T9650 224-233 VBN denotes extracted
T9652 234-238 IN denotes from
T9653 239-244 NN denotes liver
T9654 244-246 , denotes ,
T9655 246-252 NN denotes thymus
T9656 252-254 , denotes ,
T9657 254-257 CC denotes and
T9658 258-262 NN denotes skin
T9659 263-267 IN denotes from
T9660 268-271 DT denotes all
T9662 272-273 CD denotes 4
T9663 274-282 JJ denotes possible
T9661 283-292 NNS denotes genotypes
T9664 292-294 : denotes :
T9665 294-297 NN denotes K14
T9667 297-298 HYPH denotes -
T9666 298-301 NN denotes cre
T9668 301-302 : denotes ;
T9669 303-309 NN denotes ApcCKO
T9671 309-310 HYPH denotes /
T9670 310-313 NN denotes CKO
T9672 313-315 , denotes ,
T9673 315-318 NN denotes K14
T9675 318-319 HYPH denotes -
T9674 319-322 NN denotes cre
T9676 322-323 : denotes ;
T9677 324-330 NN denotes ApcCKO
T9678 330-331 HYPH denotes /
T9679 331-332 SYM denotes +
T9680 332-334 , denotes ,
T9681 334-340 NN denotes ApcCKO
T9683 340-341 HYPH denotes /
T9682 341-344 NN denotes CKO
T9684 344-346 , denotes ,
T9685 346-349 CC denotes and
T9686 350-356 NN denotes ApcCKO
T9687 356-357 HYPH denotes /
T9688 357-358 SYM denotes +
T9689 358-359 . denotes .
T9690 359-525 sentence denotes Genotyping on genomic DNA from these tissues showed that the ApcΔ580 allele (500-bp product) was detected only from the skin and thymus of the K14-cre–positive mice.
T9691 360-370 VBG denotes Genotyping
T9693 371-373 IN denotes on
T9694 374-381 JJ denotes genomic
T9695 382-385 NN denotes DNA
T9696 386-390 IN denotes from
T9697 391-396 DT denotes these
T9698 397-404 NNS denotes tissues
T9692 405-411 VBD denotes showed
T9699 412-416 IN denotes that
T9701 417-420 DT denotes the
T9703 421-428 NN denotes ApcΔ580
T9702 429-435 NN denotes allele
T9704 436-437 -LRB- denotes (
T9706 437-440 CD denotes 500
T9708 440-441 HYPH denotes -
T9707 441-443 NN denotes bp
T9705 444-451 NN denotes product
T9709 451-452 -RRB- denotes )
T9710 453-456 VBD denotes was
T9700 457-465 VBN denotes detected
T9711 466-470 RB denotes only
T9712 471-475 IN denotes from
T9713 476-479 DT denotes the
T9714 480-484 NN denotes skin
T9715 485-488 CC denotes and
T9716 489-495 NN denotes thymus
T9717 496-498 IN denotes of
T9718 499-502 DT denotes the
T9720 503-506 NN denotes K14
T9722 506-507 HYPH denotes -
T9721 507-510 NN denotes cre
T9724 510-511 HYPH denotes –
T9723 511-519 JJ denotes positive
T9719 520-524 NNS denotes mice
T9725 524-525 . denotes .
T9726 525-710 sentence denotes The presence of mutant Apc allele in the thymus of K14-cre; ApcCKO/+ mice was consistently much less than the DNA from the skin of the same animal or other tissues from the KA mutants.
T9727 526-529 DT denotes The
T9728 530-538 NN denotes presence
T9730 539-541 IN denotes of
T9731 542-548 JJ denotes mutant
T9733 549-552 NN denotes Apc
T9732 553-559 NN denotes allele
T9734 560-562 IN denotes in
T9735 563-566 DT denotes the
T9736 567-573 NN denotes thymus
T9737 574-576 IN denotes of
T9738 577-580 NN denotes K14
T9740 580-581 HYPH denotes -
T9739 581-584 NN denotes cre
T9742 584-585 : denotes ;
T9743 586-592 NN denotes ApcCKO
T9744 592-593 HYPH denotes /
T9745 593-594 SYM denotes +
T9741 595-599 NNS denotes mice
T9729 600-603 VBD denotes was
T9746 604-616 RB denotes consistently
T9747 617-621 RB denotes much
T9748 622-626 JJR denotes less
T9749 627-631 IN denotes than
T9750 632-635 DT denotes the
T9751 636-639 NN denotes DNA
T9752 640-644 IN denotes from
T9753 645-648 DT denotes the
T9754 649-653 NN denotes skin
T9755 654-656 IN denotes of
T9756 657-660 DT denotes the
T9758 661-665 JJ denotes same
T9757 666-672 NN denotes animal
T9759 673-675 CC denotes or
T9760 676-681 JJ denotes other
T9761 682-689 NNS denotes tissues
T9762 690-694 IN denotes from
T9763 695-698 DT denotes the
T9765 699-701 NN denotes KA
T9764 702-709 NNS denotes mutants
T9766 709-710 . denotes .
T9767 710-996 sentence denotes In addition, this product was not detected at all in either the liver of K14-cre–positive or in any of the K14-cre–negative mouse tissues samples, establishing that Cre-mediated recombination has taken place in the tissue-specific manner in the mice that inherited K14-cre (Figure 3A).
T9768 711-713 IN denotes In
T9770 714-722 NN denotes addition
T9771 722-724 , denotes ,
T9772 724-728 DT denotes this
T9773 729-736 NN denotes product
T9774 737-740 VBD denotes was
T9775 741-744 RB denotes not
T9769 745-753 VBN denotes detected
T9776 754-756 RB denotes at
T9777 757-760 RB denotes all
T9778 761-763 IN denotes in
T9779 764-770 CC denotes either
T9781 771-774 DT denotes the
T9780 775-780 NN denotes liver
T9782 781-783 IN denotes of
T9783 784-787 NN denotes K14
T9785 787-788 HYPH denotes -
T9784 788-791 NN denotes cre
T9787 791-792 HYPH denotes –
T9786 792-800 JJ denotes positive
T9788 801-803 CC denotes or
T9789 804-806 IN denotes in
T9790 807-810 DT denotes any
T9791 811-813 IN denotes of
T9792 814-817 DT denotes the
T9794 818-821 NN denotes K14
T9796 821-822 HYPH denotes -
T9795 822-825 NN denotes cre
T9797 825-826 HYPH denotes –
T9793 826-834 JJ denotes negative
T9798 835-840 NN denotes mouse
T9800 841-848 NNS denotes tissues
T9799 849-856 NNS denotes samples
T9801 856-858 , denotes ,
T9802 858-870 VBG denotes establishing
T9803 871-875 IN denotes that
T9805 876-879 NN denotes Cre
T9807 879-880 HYPH denotes -
T9806 880-888 JJ denotes mediated
T9808 889-902 NN denotes recombination
T9809 903-906 VBZ denotes has
T9804 907-912 VBN denotes taken
T9810 913-918 NN denotes place
T9811 919-921 IN denotes in
T9812 922-925 DT denotes the
T9814 926-932 NN denotes tissue
T9816 932-933 HYPH denotes -
T9815 933-941 JJ denotes specific
T9813 942-948 NN denotes manner
T9817 949-951 IN denotes in
T9818 952-955 DT denotes the
T9819 956-960 NNS denotes mice
T9820 961-965 WDT denotes that
T9821 966-975 VBD denotes inherited
T9822 976-979 NN denotes K14
T9824 979-980 HYPH denotes -
T9823 980-983 NN denotes cre
T9825 984-985 -LRB- denotes (
T9827 985-991 NN denotes Figure
T9826 992-994 NN denotes 3A
T9828 994-995 -RRB- denotes )
T9829 995-996 . denotes .
T9830 996-1718 sentence denotes Figure 3 Tissue-Specific Detection and Expression of Deleted Apc Alleles (A) Tissue-specific genotyping PCR. Only genomic DNA samples from the skin (S) and thymus (T), but not liver (L) of mice positive for K14-cre show the presence of deleted ApcΔ580 allele. (B) Genotype- and tissue-specific expression of the truncated Apc transcripts. A representative gel of RT-PCR using primers F546 and R721, showing that only RNA from the skin and thymus but not liver of mice positive for K14-cre have transcripts from both wild-type (528 bp) and deleted (313 bp) Apc alleles. Apc transcripts were also analyzed by RT-PCR with primers spanning exon 14 (Figure 1A) using total RNA isolated from the corresponding tissue samples.
T9831 1568-1571 NN denotes Apc
T9832 1572-1583 NNS denotes transcripts
T9834 1584-1588 VBD denotes were
T9835 1589-1593 RB denotes also
T9833 1594-1602 VBN denotes analyzed
T9836 1603-1605 IN denotes by
T9837 1606-1608 NN denotes RT
T9839 1608-1609 HYPH denotes -
T9838 1609-1612 NN denotes PCR
T9840 1613-1617 IN denotes with
T9841 1618-1625 NNS denotes primers
T9842 1626-1634 VBG denotes spanning
T9843 1635-1639 NN denotes exon
T9844 1640-1642 CD denotes 14
T9845 1643-1644 -LRB- denotes (
T9847 1644-1650 NN denotes Figure
T9846 1651-1653 NN denotes 1A
T9848 1653-1654 -RRB- denotes )
T9849 1655-1660 VBG denotes using
T9850 1661-1666 JJ denotes total
T9851 1667-1670 NN denotes RNA
T9852 1671-1679 VBN denotes isolated
T9853 1680-1684 IN denotes from
T9854 1685-1688 DT denotes the
T9856 1689-1702 VBG denotes corresponding
T9857 1703-1709 NN denotes tissue
T9855 1710-1717 NNS denotes samples
T9858 1717-1718 . denotes .
T9859 1718-1904 sentence denotes We detected the expected RT-PCR product (313 bp) from the truncated Apc (ApcΔ580) allele only in the tissues where Cre recombinase is known to be expressed in the K14-cre–positive mice.
T9860 1719-1721 PRP denotes We
T9861 1722-1730 VBD denotes detected
T9862 1731-1734 DT denotes the
T9864 1735-1743 VBN denotes expected
T9865 1744-1746 NN denotes RT
T9867 1746-1747 HYPH denotes -
T9866 1747-1750 NN denotes PCR
T9863 1751-1758 NN denotes product
T9868 1759-1760 -LRB- denotes (
T9870 1760-1763 CD denotes 313
T9869 1764-1766 NN denotes bp
T9871 1766-1767 -RRB- denotes )
T9872 1768-1772 IN denotes from
T9873 1773-1776 DT denotes the
T9875 1777-1786 VBN denotes truncated
T9876 1787-1790 NN denotes Apc
T9877 1791-1792 -LRB- denotes (
T9878 1792-1799 NN denotes ApcΔ580
T9879 1799-1800 -RRB- denotes )
T9874 1801-1807 NN denotes allele
T9880 1808-1812 RB denotes only
T9881 1813-1815 IN denotes in
T9882 1816-1819 DT denotes the
T9883 1820-1827 NNS denotes tissues
T9884 1828-1833 WRB denotes where
T9886 1834-1837 NN denotes Cre
T9887 1838-1849 NN denotes recombinase
T9888 1850-1852 VBZ denotes is
T9885 1853-1858 VBN denotes known
T9889 1859-1861 TO denotes to
T9891 1862-1864 VB denotes be
T9890 1865-1874 VBN denotes expressed
T9892 1875-1877 IN denotes in
T9893 1878-1881 DT denotes the
T9895 1882-1885 NN denotes K14
T9897 1885-1886 HYPH denotes -
T9896 1886-1889 NN denotes cre
T9899 1889-1890 HYPH denotes –
T9898 1890-1898 JJ denotes positive
T9894 1899-1903 NNS denotes mice
T9900 1903-1904 . denotes .
T9901 1904-2252 sentence denotes However, this product was not detected in either the K14-cre–negative mouse tissues samples or the liver of K14-cre–positive mice, and only the product from the wild-type allele (528 bp) was detected from these RNA samples, further confirming that Cre-mediated recombination has taken place in the tissue- and genotype-specific manner (Figure 3B).
T9902 1905-1912 RB denotes However
T9904 1912-1914 , denotes ,
T9905 1914-1918 DT denotes this
T9906 1919-1926 NN denotes product
T9907 1927-1930 VBD denotes was
T9908 1931-1934 RB denotes not
T9903 1935-1943 VBN denotes detected
T9909 1944-1946 IN denotes in
T9910 1947-1953 CC denotes either
T9912 1954-1957 DT denotes the
T9913 1958-1961 NN denotes K14
T9915 1961-1962 HYPH denotes -
T9914 1962-1965 NN denotes cre
T9917 1965-1966 HYPH denotes –
T9916 1966-1974 JJ denotes negative
T9918 1975-1980 NN denotes mouse
T9919 1981-1988 NNS denotes tissues
T9911 1989-1996 NNS denotes samples
T9920 1997-1999 CC denotes or
T9921 2000-2003 DT denotes the
T9922 2004-2009 NN denotes liver
T9923 2010-2012 IN denotes of
T9924 2013-2016 NN denotes K14
T9926 2016-2017 HYPH denotes -
T9925 2017-2020 NN denotes cre
T9928 2020-2021 HYPH denotes –
T9927 2021-2029 JJ denotes positive
T9929 2030-2034 NNS denotes mice
T9930 2034-2036 , denotes ,
T9931 2036-2039 CC denotes and
T9932 2040-2044 RB denotes only
T9934 2045-2048 DT denotes the
T9933 2049-2056 NN denotes product
T9936 2057-2061 IN denotes from
T9937 2062-2065 DT denotes the
T9939 2066-2070 JJ denotes wild
T9941 2070-2071 HYPH denotes -
T9940 2071-2075 NN denotes type
T9938 2076-2082 NN denotes allele
T9942 2083-2084 -LRB- denotes (
T9944 2084-2087 CD denotes 528
T9943 2088-2090 NN denotes bp
T9945 2090-2091 -RRB- denotes )
T9946 2092-2095 VBD denotes was
T9935 2096-2104 VBN denotes detected
T9947 2105-2109 IN denotes from
T9948 2110-2115 DT denotes these
T9950 2116-2119 NN denotes RNA
T9949 2120-2127 NNS denotes samples
T9951 2127-2129 , denotes ,
T9952 2129-2136 RB denotes further
T9953 2137-2147 VBG denotes confirming
T9954 2148-2152 IN denotes that
T9956 2153-2156 NN denotes Cre
T9958 2156-2157 HYPH denotes -
T9957 2157-2165 JJ denotes mediated
T9959 2166-2179 NN denotes recombination
T9960 2180-2183 VBZ denotes has
T9955 2184-2189 VBN denotes taken
T9961 2190-2195 NN denotes place
T9962 2196-2198 IN denotes in
T9963 2199-2202 DT denotes the
T9965 2203-2209 NN denotes tissue
T9967 2209-2210 HYPH denotes -
T9968 2211-2214 CC denotes and
T9969 2215-2223 NN denotes genotype
T9970 2223-2224 HYPH denotes -
T9966 2224-2232 JJ denotes specific
T9964 2233-2239 NN denotes manner
T9971 2240-2241 -LRB- denotes (
T9973 2241-2247 NN denotes Figure
T9972 2248-2250 NN denotes 3B
T9974 2250-2251 -RRB- denotes )
T9975 2251-2252 . denotes .
R2681 T9607 T9608 npadvmod Genotype,Specific
R2682 T9608 T9613 amod Specific,Expression
R2683 T9609 T9607 punct -,Genotype
R2684 T9610 T9607 cc and,Genotype
R2685 T9611 T9607 conj Tissue,Genotype
R2686 T9612 T9608 punct -,Specific
R2687 T9614 T9613 prep of,Expression
R2688 T9615 T9616 det the,Transcripts
R2689 T9616 T9614 pobj Transcripts,of
R2690 T9617 T9618 amod Truncated,Apc
R2691 T9618 T9616 compound Apc,Transcripts
R2692 T9620 T9621 aux To,assess
R2693 T9621 T9622 advcl assess,screened
R2694 T9623 T9624 det the,effects
R2695 T9624 T9621 dobj effects,assess
R2696 T9625 T9624 amod molecular,effects
R2697 T9626 T9624 prep of,effects
R2698 T9627 T9628 det the,recombination
R2699 T9628 T9626 pobj recombination,of
R2700 T9629 T9630 compound K14,cre
R2701 T9630 T9632 npadvmod cre,mediated
R2702 T9631 T9630 punct -,cre
R2703 T9632 T9628 amod mediated,recombination
R2704 T9633 T9632 punct –,mediated
R2705 T9634 T9622 punct ", ",screened
R2706 T9635 T9622 nsubj we,screened
R2707 T9636 T9622 prep for,screened
R2708 T9637 T9638 det the,presence
R2709 T9638 T9636 pobj presence,for
R2710 T9639 T9638 prep of,presence
R2711 T9640 T9641 amod deleted,Apc
R2712 T9641 T9642 nmod Apc,alleles
R2713 T9642 T9639 pobj alleles,of
R2714 T9643 T9641 punct (,Apc
R2715 T9644 T9641 appos ApcΔ580,Apc
R2716 T9645 T9642 punct ),alleles
R2717 T9646 T9622 punct .,screened
R2718 T9648 T9649 amod Genomic,DNA
R2719 T9649 T9650 nsubjpass DNA,extracted
R2720 T9651 T9650 auxpass was,extracted
R2721 T9652 T9650 prep from,extracted
R2722 T9653 T9652 pobj liver,from
R2723 T9654 T9653 punct ", ",liver
R2724 T9655 T9653 conj thymus,liver
R2725 T9656 T9655 punct ", ",thymus
R2726 T9657 T9655 cc and,thymus
R2727 T9658 T9655 conj skin,thymus
R2728 T9659 T9653 prep from,liver
R2729 T9660 T9661 det all,genotypes
R2730 T9661 T9659 pobj genotypes,from
R2731 T9662 T9661 nummod 4,genotypes
R2732 T9663 T9661 amod possible,genotypes
R2733 T9664 T9661 punct : ,genotypes
R2734 T9665 T9666 compound K14,cre
R2735 T9666 T9661 appos cre,genotypes
R2736 T9667 T9666 punct -,cre
R2737 T9668 T9666 punct ;,cre
R2738 T9669 T9670 compound ApcCKO,CKO
R2739 T9670 T9666 appos CKO,cre
R2740 T9671 T9670 punct /,CKO
R2741 T9672 T9666 punct ", ",cre
R2742 T9673 T9674 compound K14,cre
R2743 T9674 T9666 conj cre,cre
R2744 T9675 T9674 punct -,cre
R2745 T9676 T9674 punct ;,cre
R2746 T9677 T9674 appos ApcCKO,cre
R2747 T9678 T9677 punct /,ApcCKO
R2748 T9679 T9677 punct +,ApcCKO
R2749 T9680 T9674 punct ", ",cre
R2750 T9681 T9682 compound ApcCKO,CKO
R2751 T9682 T9674 conj CKO,cre
R2752 T9683 T9682 punct /,CKO
R2753 T9684 T9682 punct ", ",CKO
R2754 T9685 T9682 cc and,CKO
R2755 T9686 T9682 conj ApcCKO,CKO
R2756 T9687 T9686 punct /,ApcCKO
R2757 T9688 T9686 punct +,ApcCKO
R2758 T9689 T9650 punct .,extracted
R2759 T9691 T9692 nsubj Genotyping,showed
R2760 T9693 T9691 prep on,Genotyping
R2761 T9694 T9695 amod genomic,DNA
R2762 T9695 T9693 pobj DNA,on
R2763 T9696 T9695 prep from,DNA
R2764 T9697 T9698 det these,tissues
R2765 T9698 T9696 pobj tissues,from
R2766 T9699 T9700 mark that,detected
R2767 T9700 T9692 ccomp detected,showed
R2768 T9701 T9702 det the,allele
R2769 T9702 T9700 nsubjpass allele,detected
R2770 T9703 T9702 compound ApcΔ580,allele
R2771 T9704 T9705 punct (,product
R2772 T9705 T9702 parataxis product,allele
R2773 T9706 T9707 nummod 500,bp
R2774 T9707 T9705 compound bp,product
R2775 T9708 T9707 punct -,bp
R2776 T9709 T9705 punct ),product
R2777 T9710 T9700 auxpass was,detected
R2778 T9711 T9712 advmod only,from
R2779 T9712 T9700 prep from,detected
R2780 T9713 T9714 det the,skin
R2781 T9714 T9712 pobj skin,from
R2782 T9715 T9714 cc and,skin
R2783 T9716 T9714 conj thymus,skin
R2784 T9717 T9714 prep of,skin
R2785 T9718 T9719 det the,mice
R2786 T9719 T9717 pobj mice,of
R2787 T9720 T9721 compound K14,cre
R2788 T9721 T9723 npadvmod cre,positive
R2789 T9722 T9721 punct -,cre
R2790 T9723 T9719 amod positive,mice
R2791 T9724 T9723 punct –,positive
R2792 T9725 T9692 punct .,showed
R2793 T9727 T9728 det The,presence
R2794 T9728 T9729 nsubj presence,was
R2795 T9730 T9728 prep of,presence
R2796 T9731 T9732 amod mutant,allele
R2797 T9732 T9730 pobj allele,of
R2798 T9733 T9732 compound Apc,allele
R2799 T9734 T9732 prep in,allele
R2800 T9735 T9736 det the,thymus
R2801 T9736 T9734 pobj thymus,in
R2802 T9737 T9736 prep of,thymus
R2803 T9738 T9739 nmod K14,cre
R2804 T9739 T9741 nmod cre,mice
R2805 T9740 T9739 punct -,cre
R2806 T9741 T9737 pobj mice,of
R2807 T9742 T9739 punct ;,cre
R2808 T9743 T9739 appos ApcCKO,cre
R2809 T9744 T9743 punct /,ApcCKO
R2810 T9745 T9743 punct +,ApcCKO
R2811 T9746 T9729 advmod consistently,was
R2812 T9747 T9748 advmod much,less
R2813 T9748 T9729 acomp less,was
R2814 T9749 T9748 prep than,less
R2815 T9750 T9751 det the,DNA
R2816 T9751 T9749 pobj DNA,than
R2817 T9752 T9751 prep from,DNA
R2818 T9753 T9754 det the,skin
R2819 T9754 T9752 pobj skin,from
R2820 T9755 T9754 prep of,skin
R2821 T9756 T9757 det the,animal
R2822 T9757 T9755 pobj animal,of
R2823 T9758 T9757 amod same,animal
R2824 T9759 T9751 cc or,DNA
R2825 T9760 T9761 amod other,tissues
R2826 T9761 T9751 conj tissues,DNA
R2827 T9762 T9761 prep from,tissues
R2828 T9763 T9764 det the,mutants
R2829 T9764 T9762 pobj mutants,from
R2830 T9765 T9764 compound KA,mutants
R2831 T9766 T9729 punct .,was
R2832 T9768 T9769 prep In,detected
R2833 T9770 T9768 pobj addition,In
R2834 T9771 T9769 punct ", ",detected
R2835 T9772 T9773 det this,product
R2836 T9773 T9769 nsubjpass product,detected
R2837 T9774 T9769 auxpass was,detected
R2838 T9775 T9769 neg not,detected
R2839 T9776 T9777 advmod at,all
R2840 T9777 T9769 advmod all,detected
R2841 T9778 T9769 prep in,detected
R2842 T9779 T9780 preconj either,liver
R2843 T9780 T9778 pobj liver,in
R2844 T9781 T9780 det the,liver
R2845 T9782 T9780 prep of,liver
R2846 T9783 T9784 compound K14,cre
R2847 T9784 T9786 npadvmod cre,positive
R2848 T9785 T9784 punct -,cre
R2849 T9786 T9782 amod positive,of
R2850 T9787 T9786 punct –,positive
R2851 T9788 T9778 cc or,in
R2852 T9789 T9778 conj in,in
R2853 T9790 T9789 pobj any,in
R2854 T9791 T9790 prep of,any
R2855 T9792 T9793 det the,negative
R2856 T9793 T9791 pobj negative,of
R2857 T9794 T9795 compound K14,cre
R2858 T9795 T9793 npadvmod cre,negative
R2859 T9796 T9795 punct -,cre
R2860 T9797 T9793 punct –,negative
R2861 T9798 T9799 compound mouse,samples
R2862 T9799 T9789 pobj samples,in
R2863 T9800 T9799 compound tissues,samples
R2864 T9801 T9769 punct ", ",detected
R2865 T9802 T9769 advcl establishing,detected
R2866 T9803 T9804 mark that,taken
R2867 T9804 T9802 ccomp taken,establishing
R2868 T9805 T9806 npadvmod Cre,mediated
R2869 T9806 T9808 amod mediated,recombination
R2870 T9807 T9806 punct -,mediated
R2871 T9808 T9804 nsubj recombination,taken
R2872 T9809 T9804 aux has,taken
R2873 T9810 T9804 dobj place,taken
R2874 T9811 T9804 prep in,taken
R2875 T9812 T9813 det the,manner
R2876 T9813 T9811 pobj manner,in
R2877 T9814 T9815 npadvmod tissue,specific
R2878 T9815 T9813 amod specific,manner
R2879 T9816 T9815 punct -,specific
R2880 T9817 T9804 prep in,taken
R2881 T9818 T9819 det the,mice
R2882 T9819 T9817 pobj mice,in
R2883 T9820 T9821 dep that,inherited
R2884 T9821 T9819 relcl inherited,mice
R2885 T9822 T9823 compound K14,cre
R2886 T9823 T9821 dobj cre,inherited
R2887 T9824 T9823 punct -,cre
R2888 T9825 T9826 punct (,3A
R2889 T9826 T9769 parataxis 3A,detected
R2890 T9827 T9826 compound Figure,3A
R2891 T9828 T9826 punct ),3A
R2892 T9829 T9769 punct .,detected
R2893 T9831 T9832 compound Apc,transcripts
R2894 T9832 T9833 nsubjpass transcripts,analyzed
R2895 T9834 T9833 auxpass were,analyzed
R2896 T9835 T9833 advmod also,analyzed
R2897 T9836 T9833 prep by,analyzed
R2898 T9837 T9838 compound RT,PCR
R2899 T9838 T9836 pobj PCR,by
R2900 T9839 T9838 punct -,PCR
R2901 T9840 T9838 prep with,PCR
R2902 T9841 T9840 pobj primers,with
R2903 T9842 T9841 acl spanning,primers
R2904 T9843 T9842 dobj exon,spanning
R2905 T9844 T9843 nummod 14,exon
R2906 T9845 T9846 punct (,1A
R2907 T9846 T9833 parataxis 1A,analyzed
R2908 T9847 T9846 compound Figure,1A
R2909 T9848 T9846 punct ),1A
R2910 T9849 T9833 advcl using,analyzed
R2911 T9850 T9851 amod total,RNA
R2912 T9851 T9849 dobj RNA,using
R2913 T9852 T9851 acl isolated,RNA
R2914 T9853 T9852 prep from,isolated
R2915 T9854 T9855 det the,samples
R2916 T9855 T9853 pobj samples,from
R2917 T9856 T9855 amod corresponding,samples
R2918 T9857 T9855 compound tissue,samples
R2919 T9858 T9833 punct .,analyzed
R2920 T9860 T9861 nsubj We,detected
R2921 T9862 T9863 det the,product
R2922 T9863 T9861 dobj product,detected
R2923 T9864 T9863 amod expected,product
R2924 T9865 T9866 compound RT,PCR
R2925 T9866 T9863 compound PCR,product
R2926 T9867 T9866 punct -,PCR
R2927 T9868 T9869 punct (,bp
R2928 T9869 T9863 parataxis bp,product
R2929 T9870 T9869 nummod 313,bp
R2930 T9871 T9869 punct ),bp
R2931 T9872 T9863 prep from,product
R2932 T9873 T9874 det the,allele
R2933 T9874 T9872 pobj allele,from
R2934 T9875 T9876 amod truncated,Apc
R2935 T9876 T9874 nmod Apc,allele
R2936 T9877 T9876 punct (,Apc
R2937 T9878 T9876 appos ApcΔ580,Apc
R2938 T9879 T9874 punct ),allele
R2939 T9880 T9881 advmod only,in
R2940 T9881 T9861 prep in,detected
R2941 T9882 T9883 det the,tissues
R2942 T9883 T9881 pobj tissues,in
R2943 T9884 T9885 advmod where,known
R2944 T9885 T9883 advcl known,tissues
R2945 T9886 T9887 compound Cre,recombinase
R2946 T9887 T9885 nsubjpass recombinase,known
R2947 T9888 T9885 auxpass is,known
R2948 T9889 T9890 aux to,expressed
R2949 T9890 T9885 xcomp expressed,known
R2950 T9891 T9890 auxpass be,expressed
R2951 T9892 T9890 prep in,expressed
R2952 T9893 T9894 det the,mice
R2953 T9894 T9892 pobj mice,in
R2954 T9895 T9896 compound K14,cre
R2955 T9896 T9898 npadvmod cre,positive
R2956 T9897 T9896 punct -,cre
R2957 T9898 T9894 amod positive,mice
R2958 T9899 T9898 punct –,positive
R2959 T9900 T9861 punct .,detected
R2960 T9902 T9903 advmod However,detected
R2961 T9904 T9903 punct ", ",detected
R2962 T9905 T9906 det this,product
R2963 T9906 T9903 nsubjpass product,detected
R2964 T9907 T9903 auxpass was,detected
R2965 T9908 T9903 neg not,detected
R2966 T9909 T9903 prep in,detected
R2967 T9910 T9911 preconj either,samples
R2968 T9911 T9909 pobj samples,in
R2969 T9912 T9911 det the,samples
R2970 T9913 T9914 compound K14,cre
R2971 T9914 T9916 npadvmod cre,negative
R2972 T9915 T9914 punct -,cre
R2973 T9916 T9911 amod negative,samples
R2974 T9917 T9916 punct –,negative
R2975 T9918 T9919 compound mouse,tissues
R2976 T9919 T9911 compound tissues,samples
R2977 T9920 T9911 cc or,samples
R2978 T9921 T9922 det the,liver
R2979 T9922 T9911 conj liver,samples
R2980 T9923 T9922 prep of,liver
R2981 T9924 T9925 compound K14,cre
R2982 T9925 T9927 npadvmod cre,positive
R2983 T9926 T9925 punct -,cre
R2984 T9927 T9929 amod positive,mice
R2985 T9928 T9927 punct –,positive
R2986 T9929 T9923 pobj mice,of
R2987 T9930 T9903 punct ", ",detected
R2988 T9931 T9903 cc and,detected
R2989 T9932 T9933 advmod only,product
R2990 T9933 T9935 nsubjpass product,detected
R2991 T9934 T9933 det the,product
R2992 T9935 T9903 conj detected,detected
R2993 T9936 T9933 prep from,product
R2994 T9937 T9938 det the,allele
R2995 T9938 T9936 pobj allele,from
R2996 T9939 T9940 amod wild,type
R2997 T9940 T9938 compound type,allele
R2998 T9941 T9940 punct -,type
R2999 T9942 T9943 punct (,bp
R3000 T9943 T9933 parataxis bp,product
R3001 T9944 T9943 nummod 528,bp
R3002 T9945 T9943 punct ),bp
R3003 T9946 T9935 auxpass was,detected
R3004 T9947 T9935 prep from,detected
R3005 T9948 T9949 det these,samples
R3006 T9949 T9947 pobj samples,from
R3007 T9950 T9949 compound RNA,samples
R3008 T9951 T9935 punct ", ",detected
R3009 T9952 T9953 advmod further,confirming
R3010 T9953 T9935 advcl confirming,detected
R3011 T9954 T9955 mark that,taken
R3012 T9955 T9953 ccomp taken,confirming
R3013 T9956 T9957 npadvmod Cre,mediated
R3014 T9957 T9959 amod mediated,recombination
R3015 T9958 T9957 punct -,mediated
R3016 T9959 T9955 nsubj recombination,taken
R3017 T9960 T9955 aux has,taken
R3018 T9961 T9955 dobj place,taken
R3019 T9962 T9955 prep in,taken
R3020 T9963 T9964 det the,manner
R3021 T9964 T9962 pobj manner,in
R3022 T9965 T9966 npadvmod tissue,specific
R3023 T9966 T9964 amod specific,manner
R3024 T9967 T9965 punct -,tissue
R3025 T9968 T9965 cc and,tissue
R3026 T9969 T9965 conj genotype,tissue
R3027 T9970 T9966 punct -,specific
R3028 T9971 T9972 punct (,3B
R3029 T9972 T9935 parataxis 3B,detected
R3030 T9973 T9972 compound Figure,3B
R3031 T9974 T9972 punct ),3B
R3032 T9975 T9903 punct .,detected