| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T8074 |
0-5 |
NNS |
denotes |
Cells |
| T8076 |
5-7 |
, |
denotes |
, |
| T8077 |
7-16 |
JJ |
denotes |
untreated |
| T8078 |
17-19 |
CC |
denotes |
or |
| T8079 |
20-27 |
VBN |
denotes |
treated |
| T8080 |
28-31 |
IN |
denotes |
for |
| T8081 |
32-34 |
CD |
denotes |
24 |
| T8082 |
35-36 |
NN |
denotes |
h |
| T8083 |
37-41 |
IN |
denotes |
with |
| T8084 |
42-45 |
CD |
denotes |
0.5 |
| T8085 |
46-48 |
NN |
denotes |
μg |
| T8087 |
48-49 |
SYM |
denotes |
/ |
| T8088 |
49-51 |
NN |
denotes |
ml |
| T8086 |
52-62 |
NN |
denotes |
adriamycin |
| T8089 |
62-64 |
, |
denotes |
, |
| T8090 |
64-68 |
VBD |
denotes |
were |
| T8075 |
69-74 |
VBN |
denotes |
lysed |
| T8091 |
75-77 |
IN |
denotes |
on |
| T8092 |
78-81 |
DT |
denotes |
the |
| T8093 |
82-86 |
NN |
denotes |
dish |
| T8094 |
87-89 |
IN |
denotes |
in |
| T8095 |
90-91 |
DT |
denotes |
a |
| T8096 |
92-98 |
NN |
denotes |
buffer |
| T8097 |
99-109 |
VBG |
denotes |
consisting |
| T8098 |
110-112 |
IN |
denotes |
of |
| T8099 |
113-115 |
CD |
denotes |
50 |
| T8100 |
116-118 |
NN |
denotes |
mM |
| T8101 |
119-123 |
NN |
denotes |
Tris |
| T8102 |
124-125 |
-LRB- |
denotes |
( |
| T8103 |
125-127 |
NN |
denotes |
pH |
| T8104 |
128-131 |
CD |
denotes |
8.0 |
| T8105 |
131-132 |
-RRB- |
denotes |
) |
| T8106 |
132-134 |
, |
denotes |
, |
| T8107 |
134-135 |
CD |
denotes |
5 |
| T8108 |
136-138 |
NN |
denotes |
mM |
| T8109 |
139-143 |
NN |
denotes |
EDTA |
| T8110 |
143-145 |
, |
denotes |
, |
| T8111 |
145-148 |
CD |
denotes |
150 |
| T8112 |
149-151 |
NN |
denotes |
mM |
| T8113 |
152-156 |
NN |
denotes |
NaCl |
| T8114 |
156-158 |
, |
denotes |
, |
| T8115 |
158-161 |
CD |
denotes |
0.5 |
| T8116 |
161-162 |
NN |
denotes |
% |
| T8118 |
163-170 |
NN |
denotes |
Nonidet |
| T8119 |
171-172 |
NN |
denotes |
P |
| T8120 |
172-173 |
HYPH |
denotes |
- |
| T8117 |
173-175 |
NN |
denotes |
40 |
| T8121 |
175-177 |
, |
denotes |
, |
| T8122 |
177-178 |
CD |
denotes |
1 |
| T8123 |
179-181 |
NN |
denotes |
mM |
| T8124 |
182-186 |
NN |
denotes |
PMSF |
| T8125 |
186-188 |
, |
denotes |
, |
| T8126 |
188-189 |
CD |
denotes |
1 |
| T8127 |
190-192 |
NN |
denotes |
mM |
| T8129 |
193-199 |
NN |
denotes |
sodium |
| T8128 |
200-208 |
NN |
denotes |
vanadate |
| T8130 |
208-210 |
, |
denotes |
, |
| T8131 |
210-212 |
CD |
denotes |
10 |
| T8132 |
213-215 |
NN |
denotes |
mM |
| T8133 |
216-219 |
NNP |
denotes |
NaF |
| T8134 |
220-223 |
CC |
denotes |
and |
| T8135 |
224-232 |
NN |
denotes |
Complete |
| T8136 |
233-237 |
NN |
denotes |
Mini |
| T8138 |
238-246 |
NN |
denotes |
Protease |
| T8137 |
247-257 |
NNS |
denotes |
Inhibitors |
| T8139 |
258-259 |
-LRB- |
denotes |
( |
| T8141 |
259-264 |
NNP |
denotes |
Roche |
| T8140 |
265-276 |
NNP |
denotes |
Diagnostics |
| T8142 |
276-277 |
-RRB- |
denotes |
) |
| T8143 |
278-280 |
IN |
denotes |
at |
| T8144 |
281-282 |
CD |
denotes |
4 |
| T8145 |
282-284 |
NNS |
denotes |
°C |
| T8146 |
285-288 |
IN |
denotes |
for |
| T8147 |
289-291 |
CD |
denotes |
30 |
| T8148 |
292-295 |
NN |
denotes |
min |
| T8149 |
295-296 |
. |
denotes |
. |
| T8150 |
296-358 |
sentence |
denotes |
Lysates were scraped, then spun at 6000× g at 4°C for 10 min. |
| T8151 |
297-304 |
NNS |
denotes |
Lysates |
| T8153 |
305-309 |
VBD |
denotes |
were |
| T8152 |
310-317 |
VBN |
denotes |
scraped |
| T8154 |
317-319 |
, |
denotes |
, |
| T8155 |
319-323 |
RB |
denotes |
then |
| T8156 |
324-328 |
VBN |
denotes |
spun |
| T8157 |
329-331 |
IN |
denotes |
at |
| T8158 |
332-336 |
CD |
denotes |
6000 |
| T8160 |
336-337 |
SYM |
denotes |
× |
| T8159 |
338-339 |
NN |
denotes |
g |
| T8161 |
340-342 |
IN |
denotes |
at |
| T8162 |
343-344 |
CD |
denotes |
4 |
| T8163 |
344-346 |
NNS |
denotes |
°C |
| T8164 |
347-350 |
IN |
denotes |
for |
| T8165 |
351-353 |
CD |
denotes |
10 |
| T8166 |
354-357 |
NN |
denotes |
min |
| T8167 |
357-358 |
. |
denotes |
. |
| T8168 |
358-450 |
sentence |
denotes |
Protein concentration in the supernatant was determined using the Bio-Rad DC protein assay. |
| T8169 |
359-366 |
NN |
denotes |
Protein |
| T8170 |
367-380 |
NN |
denotes |
concentration |
| T8172 |
381-383 |
IN |
denotes |
in |
| T8173 |
384-387 |
DT |
denotes |
the |
| T8174 |
388-399 |
NN |
denotes |
supernatant |
| T8175 |
400-403 |
VBD |
denotes |
was |
| T8171 |
404-414 |
VBN |
denotes |
determined |
| T8176 |
415-420 |
VBG |
denotes |
using |
| T8177 |
421-424 |
DT |
denotes |
the |
| T8179 |
425-428 |
NN |
denotes |
Bio |
| T8181 |
428-429 |
HYPH |
denotes |
- |
| T8180 |
429-432 |
NN |
denotes |
Rad |
| T8182 |
433-435 |
NN |
denotes |
DC |
| T8183 |
436-443 |
NN |
denotes |
protein |
| T8178 |
444-449 |
NN |
denotes |
assay |
| T8184 |
449-450 |
. |
denotes |
. |
| T8185 |
450-605 |
sentence |
denotes |
Lysates were separated on single percentage SDS/PAGE gels, then electrophoretically transferred to poly(vinylidene difluoride), using standard procedures. |
| T8186 |
451-458 |
NNS |
denotes |
Lysates |
| T8188 |
459-463 |
VBD |
denotes |
were |
| T8187 |
464-473 |
VBN |
denotes |
separated |
| T8189 |
474-476 |
IN |
denotes |
on |
| T8190 |
477-483 |
JJ |
denotes |
single |
| T8191 |
484-494 |
NN |
denotes |
percentage |
| T8193 |
495-498 |
NN |
denotes |
SDS |
| T8195 |
498-499 |
HYPH |
denotes |
/ |
| T8194 |
499-503 |
NN |
denotes |
PAGE |
| T8192 |
504-508 |
NNS |
denotes |
gels |
| T8196 |
508-510 |
, |
denotes |
, |
| T8197 |
510-514 |
RB |
denotes |
then |
| T8199 |
515-534 |
RB |
denotes |
electrophoretically |
| T8198 |
535-546 |
VBN |
denotes |
transferred |
| T8200 |
547-549 |
IN |
denotes |
to |
| T8201 |
550-554 |
NN |
denotes |
poly |
| T8202 |
554-555 |
-LRB- |
denotes |
( |
| T8204 |
555-565 |
NN |
denotes |
vinylidene |
| T8203 |
566-576 |
NN |
denotes |
difluoride |
| T8205 |
576-577 |
-RRB- |
denotes |
) |
| T8206 |
577-579 |
, |
denotes |
, |
| T8207 |
579-584 |
VBG |
denotes |
using |
| T8208 |
585-593 |
JJ |
denotes |
standard |
| T8209 |
594-604 |
NNS |
denotes |
procedures |
| T8210 |
604-605 |
. |
denotes |
. |
| T8211 |
605-610 |
NNS |
denotes |
Blots |
| T8213 |
605-824 |
sentence |
denotes |
Blots were incubated in 5% non-fat dried milk in TBST (0.02 M Tris, pH 7.6/0.35 M NaCl/0.1% Tween-20) for 1 h at room temperature before probing with primary antibodies against p53 (CM-5, Novacastra) and -actin (Sigma). |
| T8214 |
611-615 |
VBD |
denotes |
were |
| T8212 |
616-625 |
VBN |
denotes |
incubated |
| T8215 |
626-628 |
IN |
denotes |
in |
| T8216 |
629-630 |
CD |
denotes |
5 |
| T8217 |
630-631 |
NN |
denotes |
% |
| T8219 |
632-639 |
JJ |
denotes |
non-fat |
| T8220 |
640-645 |
VBN |
denotes |
dried |
| T8218 |
646-650 |
NN |
denotes |
milk |
| T8221 |
651-653 |
IN |
denotes |
in |
| T8222 |
654-658 |
NN |
denotes |
TBST |
| T8223 |
659-660 |
-LRB- |
denotes |
( |
| T8225 |
660-664 |
CD |
denotes |
0.02 |
| T8226 |
665-666 |
NN |
denotes |
M |
| T8227 |
667-671 |
NN |
denotes |
Tris |
| T8228 |
671-673 |
, |
denotes |
, |
| T8229 |
673-675 |
NN |
denotes |
pH |
| T8230 |
676-679 |
CD |
denotes |
7.6 |
| T8231 |
679-680 |
HYPH |
denotes |
/ |
| T8232 |
680-684 |
CD |
denotes |
0.35 |
| T8233 |
685-686 |
NN |
denotes |
M |
| T8234 |
687-691 |
NN |
denotes |
NaCl |
| T8235 |
691-692 |
HYPH |
denotes |
/ |
| T8236 |
692-695 |
CD |
denotes |
0.1 |
| T8237 |
695-696 |
NN |
denotes |
% |
| T8224 |
697-702 |
NN |
denotes |
Tween |
| T8238 |
702-703 |
HYPH |
denotes |
- |
| T8239 |
703-705 |
CD |
denotes |
20 |
| T8240 |
705-706 |
-RRB- |
denotes |
) |
| T8241 |
707-710 |
IN |
denotes |
for |
| T8242 |
711-712 |
CD |
denotes |
1 |
| T8243 |
713-714 |
NN |
denotes |
h |
| T8244 |
715-717 |
IN |
denotes |
at |
| T8245 |
718-722 |
NN |
denotes |
room |
| T8246 |
723-734 |
NN |
denotes |
temperature |
| T8247 |
735-741 |
IN |
denotes |
before |
| T8248 |
742-749 |
VBG |
denotes |
probing |
| T8249 |
750-754 |
IN |
denotes |
with |
| T8250 |
755-762 |
JJ |
denotes |
primary |
| T8251 |
763-773 |
NNS |
denotes |
antibodies |
| T8252 |
774-781 |
IN |
denotes |
against |
| T8253 |
782-785 |
NN |
denotes |
p53 |
| T8254 |
786-787 |
-LRB- |
denotes |
( |
| T8256 |
787-789 |
NN |
denotes |
CM |
| T8257 |
789-790 |
HYPH |
denotes |
- |
| T8258 |
790-791 |
CD |
denotes |
5 |
| T8259 |
791-793 |
, |
denotes |
, |
| T8255 |
793-803 |
NNP |
denotes |
Novacastra |
| T8260 |
803-804 |
-RRB- |
denotes |
) |
| T8261 |
805-808 |
CC |
denotes |
and |
| T8262 |
809-810 |
HYPH |
denotes |
- |
| T8263 |
810-815 |
NN |
denotes |
actin |
| T8264 |
816-817 |
-LRB- |
denotes |
( |
| T8265 |
817-822 |
NNP |
denotes |
Sigma |
| T8266 |
822-823 |
-RRB- |
denotes |
) |
| T8267 |
823-824 |
. |
denotes |
. |
| T8268 |
824-930 |
sentence |
denotes |
Secondary antibodies used include peroxidase-conjugated goat anti-mouse IgG and anti-rabbit IgG (Pierce). |
| T8269 |
825-834 |
JJ |
denotes |
Secondary |
| T8270 |
835-845 |
NNS |
denotes |
antibodies |
| T8272 |
846-850 |
VBN |
denotes |
used |
| T8271 |
851-858 |
VBP |
denotes |
include |
| T8273 |
859-869 |
NN |
denotes |
peroxidase |
| T8275 |
869-870 |
HYPH |
denotes |
- |
| T8274 |
870-880 |
VBN |
denotes |
conjugated |
| T8277 |
881-885 |
NN |
denotes |
goat |
| T8278 |
886-896 |
NN |
denotes |
anti-mouse |
| T8276 |
897-900 |
NN |
denotes |
IgG |
| T8279 |
901-904 |
CC |
denotes |
and |
| T8280 |
905-916 |
NN |
denotes |
anti-rabbit |
| T8281 |
917-920 |
NN |
denotes |
IgG |
| T8282 |
921-922 |
-LRB- |
denotes |
( |
| T8283 |
922-928 |
NNP |
denotes |
Pierce |
| T8284 |
928-929 |
-RRB- |
denotes |
) |
| T8285 |
929-930 |
. |
denotes |
. |
| T8286 |
930-1047 |
sentence |
denotes |
Probed blots were incubated with Pierce Supersignal West Pico chemiluminescent substrate and exposed to X-ray films. |
| T8287 |
931-937 |
VBN |
denotes |
Probed |
| T8288 |
938-943 |
NNS |
denotes |
blots |
| T8290 |
944-948 |
VBD |
denotes |
were |
| T8289 |
949-958 |
VBN |
denotes |
incubated |
| T8291 |
959-963 |
IN |
denotes |
with |
| T8292 |
964-970 |
NNP |
denotes |
Pierce |
| T8294 |
971-982 |
NNP |
denotes |
Supersignal |
| T8295 |
983-987 |
NNP |
denotes |
West |
| T8296 |
988-992 |
NNP |
denotes |
Pico |
| T8297 |
993-1009 |
JJ |
denotes |
chemiluminescent |
| T8293 |
1010-1019 |
NN |
denotes |
substrate |
| T8298 |
1020-1023 |
CC |
denotes |
and |
| T8299 |
1024-1031 |
VBN |
denotes |
exposed |
| T8300 |
1032-1034 |
IN |
denotes |
to |
| T8301 |
1035-1040 |
NN |
denotes |
X-ray |
| T8302 |
1041-1046 |
NNS |
denotes |
films |
| T8303 |
1046-1047 |
. |
denotes |
. |
| R2375 |
T8074 |
T8075 |
nsubjpass |
Cells,lysed |
| R2376 |
T8076 |
T8074 |
punct |
", ",Cells |
| R2377 |
T8077 |
T8074 |
amod |
untreated,Cells |
| R2378 |
T8078 |
T8077 |
cc |
or,untreated |
| R2379 |
T8079 |
T8077 |
conj |
treated,untreated |
| R2380 |
T8080 |
T8079 |
prep |
for,treated |
| R2381 |
T8081 |
T8082 |
nummod |
24,h |
| R2382 |
T8082 |
T8080 |
pobj |
h,for |
| R2383 |
T8083 |
T8079 |
prep |
with,treated |
| R2384 |
T8084 |
T8085 |
nummod |
0.5,μg |
| R2385 |
T8085 |
T8086 |
nmod |
μg,adriamycin |
| R2386 |
T8086 |
T8083 |
pobj |
adriamycin,with |
| R2387 |
T8087 |
T8088 |
punct |
/,ml |
| R2388 |
T8088 |
T8085 |
prep |
ml,μg |
| R2389 |
T8089 |
T8075 |
punct |
", ",lysed |
| R2390 |
T8090 |
T8075 |
auxpass |
were,lysed |
| R2391 |
T8091 |
T8075 |
prep |
on,lysed |
| R2392 |
T8092 |
T8093 |
det |
the,dish |
| R2393 |
T8093 |
T8091 |
pobj |
dish,on |
| R2394 |
T8094 |
T8075 |
prep |
in,lysed |
| R2395 |
T8095 |
T8096 |
det |
a,buffer |
| R2396 |
T8096 |
T8094 |
pobj |
buffer,in |
| R2397 |
T8097 |
T8096 |
acl |
consisting,buffer |
| R2398 |
T8098 |
T8097 |
prep |
of,consisting |
| R2399 |
T8099 |
T8100 |
nummod |
50,mM |
| R2400 |
T8100 |
T8101 |
compound |
mM,Tris |
| R2401 |
T8101 |
T8098 |
pobj |
Tris,of |
| R2402 |
T8102 |
T8103 |
punct |
(,pH |
| R2403 |
T8103 |
T8101 |
parataxis |
pH,Tris |
| R2404 |
T8104 |
T8103 |
nummod |
8.0,pH |
| R2405 |
T8105 |
T8103 |
punct |
),pH |
| R2406 |
T8106 |
T8101 |
punct |
", ",Tris |
| R2407 |
T8107 |
T8108 |
nummod |
5,mM |
| R2408 |
T8108 |
T8109 |
compound |
mM,EDTA |
| R2409 |
T8109 |
T8101 |
conj |
EDTA,Tris |
| R2410 |
T8110 |
T8109 |
punct |
", ",EDTA |
| R2411 |
T8111 |
T8112 |
nummod |
150,mM |
| R2412 |
T8112 |
T8113 |
compound |
mM,NaCl |
| R2413 |
T8113 |
T8109 |
conj |
NaCl,EDTA |
| R2414 |
T8114 |
T8113 |
punct |
", ",NaCl |
| R2415 |
T8115 |
T8116 |
nummod |
0.5,% |
| R2416 |
T8116 |
T8117 |
compound |
%,40 |
| R2417 |
T8117 |
T8113 |
conj |
40,NaCl |
| R2418 |
T8118 |
T8117 |
compound |
Nonidet,40 |
| R2419 |
T8119 |
T8117 |
compound |
P,40 |
| R2420 |
T8120 |
T8117 |
punct |
-,40 |
| R2421 |
T8121 |
T8117 |
punct |
", ",40 |
| R2422 |
T8122 |
T8123 |
nummod |
1,mM |
| R2423 |
T8123 |
T8124 |
compound |
mM,PMSF |
| R2424 |
T8124 |
T8117 |
conj |
PMSF,40 |
| R2425 |
T8125 |
T8124 |
punct |
", ",PMSF |
| R2426 |
T8126 |
T8127 |
nummod |
1,mM |
| R2427 |
T8127 |
T8128 |
compound |
mM,vanadate |
| R2428 |
T8128 |
T8124 |
conj |
vanadate,PMSF |
| R2429 |
T8129 |
T8128 |
compound |
sodium,vanadate |
| R2430 |
T8130 |
T8128 |
punct |
", ",vanadate |
| R2431 |
T8131 |
T8132 |
nummod |
10,mM |
| R2432 |
T8132 |
T8133 |
compound |
mM,NaF |
| R2433 |
T8133 |
T8128 |
conj |
NaF,vanadate |
| R2434 |
T8134 |
T8133 |
cc |
and,NaF |
| R2435 |
T8135 |
T8136 |
compound |
Complete,Mini |
| R2436 |
T8136 |
T8137 |
compound |
Mini,Inhibitors |
| R2437 |
T8137 |
T8133 |
conj |
Inhibitors,NaF |
| R2438 |
T8138 |
T8137 |
compound |
Protease,Inhibitors |
| R2439 |
T8139 |
T8140 |
punct |
(,Diagnostics |
| R2440 |
T8140 |
T8137 |
parataxis |
Diagnostics,Inhibitors |
| R2441 |
T8141 |
T8140 |
compound |
Roche,Diagnostics |
| R2442 |
T8142 |
T8140 |
punct |
),Diagnostics |
| R2443 |
T8143 |
T8075 |
prep |
at,lysed |
| R2444 |
T8144 |
T8145 |
nummod |
4,°C |
| R2445 |
T8145 |
T8143 |
pobj |
°C,at |
| R2446 |
T8146 |
T8075 |
prep |
for,lysed |
| R2447 |
T8147 |
T8148 |
nummod |
30,min |
| R2448 |
T8148 |
T8146 |
pobj |
min,for |
| R2449 |
T8149 |
T8075 |
punct |
.,lysed |
| R2450 |
T8151 |
T8152 |
nsubjpass |
Lysates,scraped |
| R2451 |
T8153 |
T8152 |
auxpass |
were,scraped |
| R2452 |
T8154 |
T8152 |
punct |
", ",scraped |
| R2453 |
T8155 |
T8156 |
advmod |
then,spun |
| R2454 |
T8156 |
T8152 |
dep |
spun,scraped |
| R2455 |
T8157 |
T8156 |
prep |
at,spun |
| R2456 |
T8158 |
T8159 |
nummod |
6000,g |
| R2457 |
T8159 |
T8157 |
pobj |
g,at |
| R2458 |
T8160 |
T8159 |
punct |
×,g |
| R2459 |
T8161 |
T8156 |
prep |
at,spun |
| R2460 |
T8162 |
T8163 |
nummod |
4,°C |
| R2461 |
T8163 |
T8161 |
pobj |
°C,at |
| R2462 |
T8164 |
T8156 |
prep |
for,spun |
| R2463 |
T8165 |
T8166 |
nummod |
10,min |
| R2464 |
T8166 |
T8164 |
pobj |
min,for |
| R2465 |
T8167 |
T8152 |
punct |
.,scraped |
| R2466 |
T8169 |
T8170 |
compound |
Protein,concentration |
| R2467 |
T8170 |
T8171 |
nsubjpass |
concentration,determined |
| R2468 |
T8172 |
T8170 |
prep |
in,concentration |
| R2469 |
T8173 |
T8174 |
det |
the,supernatant |
| R2470 |
T8174 |
T8172 |
pobj |
supernatant,in |
| R2471 |
T8175 |
T8171 |
auxpass |
was,determined |
| R2472 |
T8176 |
T8171 |
advcl |
using,determined |
| R2473 |
T8177 |
T8178 |
det |
the,assay |
| R2474 |
T8178 |
T8176 |
dobj |
assay,using |
| R2475 |
T8179 |
T8180 |
compound |
Bio,Rad |
| R2476 |
T8180 |
T8178 |
compound |
Rad,assay |
| R2477 |
T8181 |
T8180 |
punct |
-,Rad |
| R2478 |
T8182 |
T8178 |
compound |
DC,assay |
| R2479 |
T8183 |
T8178 |
compound |
protein,assay |
| R2480 |
T8184 |
T8171 |
punct |
.,determined |
| R2481 |
T8186 |
T8187 |
nsubjpass |
Lysates,separated |
| R2482 |
T8188 |
T8187 |
auxpass |
were,separated |
| R2483 |
T8189 |
T8187 |
prep |
on,separated |
| R2484 |
T8190 |
T8191 |
amod |
single,percentage |
| R2485 |
T8191 |
T8192 |
compound |
percentage,gels |
| R2486 |
T8192 |
T8189 |
pobj |
gels,on |
| R2487 |
T8193 |
T8194 |
compound |
SDS,PAGE |
| R2488 |
T8194 |
T8192 |
compound |
PAGE,gels |
| R2489 |
T8195 |
T8194 |
punct |
/,PAGE |
| R2490 |
T8196 |
T8187 |
punct |
", ",separated |
| R2491 |
T8197 |
T8198 |
advmod |
then,transferred |
| R2492 |
T8198 |
T8187 |
dep |
transferred,separated |
| R2493 |
T8199 |
T8198 |
advmod |
electrophoretically,transferred |
| R2494 |
T8200 |
T8198 |
prep |
to,transferred |
| R2495 |
T8201 |
T8200 |
pobj |
poly,to |
| R2496 |
T8202 |
T8203 |
punct |
(,difluoride |
| R2497 |
T8203 |
T8201 |
parataxis |
difluoride,poly |
| R2498 |
T8204 |
T8203 |
compound |
vinylidene,difluoride |
| R2499 |
T8205 |
T8203 |
punct |
),difluoride |
| R2500 |
T8206 |
T8198 |
punct |
", ",transferred |
| R2501 |
T8207 |
T8198 |
advcl |
using,transferred |
| R2502 |
T8208 |
T8209 |
amod |
standard,procedures |
| R2503 |
T8209 |
T8207 |
dobj |
procedures,using |
| R2504 |
T8210 |
T8187 |
punct |
.,separated |
| R2505 |
T8211 |
T8212 |
nsubjpass |
Blots,incubated |
| R2506 |
T8214 |
T8212 |
auxpass |
were,incubated |
| R2507 |
T8215 |
T8212 |
prep |
in,incubated |
| R2508 |
T8216 |
T8217 |
nummod |
5,% |
| R2509 |
T8217 |
T8218 |
nmod |
%,milk |
| R2510 |
T8218 |
T8215 |
pobj |
milk,in |
| R2511 |
T8219 |
T8218 |
amod |
non-fat,milk |
| R2512 |
T8220 |
T8218 |
amod |
dried,milk |
| R2513 |
T8221 |
T8212 |
prep |
in,incubated |
| R2514 |
T8222 |
T8221 |
pobj |
TBST,in |
| R2515 |
T8223 |
T8224 |
punct |
(,Tween |
| R2516 |
T8224 |
T8222 |
parataxis |
Tween,TBST |
| R2517 |
T8225 |
T8226 |
nummod |
0.02,M |
| R2518 |
T8226 |
T8227 |
compound |
M,Tris |
| R2519 |
T8227 |
T8224 |
dep |
Tris,Tween |
| R2520 |
T8228 |
T8224 |
punct |
", ",Tween |
| R2521 |
T8229 |
T8224 |
nmod |
pH,Tween |
| R2522 |
T8230 |
T8229 |
nummod |
7.6,pH |
| R2523 |
T8231 |
T8224 |
punct |
/,Tween |
| R2524 |
T8232 |
T8233 |
nummod |
0.35,M |
| R2525 |
T8233 |
T8234 |
nmod |
M,NaCl |
| R2526 |
T8234 |
T8224 |
nmod |
NaCl,Tween |
| R2527 |
T8235 |
T8224 |
punct |
/,Tween |
| R2528 |
T8236 |
T8237 |
nummod |
0.1,% |
| R2529 |
T8237 |
T8224 |
compound |
%,Tween |
| R2530 |
T8238 |
T8224 |
punct |
-,Tween |
| R2531 |
T8239 |
T8224 |
nummod |
20,Tween |
| R2532 |
T8240 |
T8224 |
punct |
),Tween |
| R2533 |
T8241 |
T8212 |
prep |
for,incubated |
| R2534 |
T8242 |
T8243 |
nummod |
1,h |
| R2535 |
T8243 |
T8241 |
pobj |
h,for |
| R2536 |
T8244 |
T8212 |
prep |
at,incubated |
| R2537 |
T8245 |
T8246 |
compound |
room,temperature |
| R2538 |
T8246 |
T8244 |
pobj |
temperature,at |
| R2539 |
T8247 |
T8212 |
prep |
before,incubated |
| R2540 |
T8248 |
T8247 |
pcomp |
probing,before |
| R2541 |
T8249 |
T8248 |
prep |
with,probing |
| R2542 |
T8250 |
T8251 |
amod |
primary,antibodies |
| R2543 |
T8251 |
T8249 |
pobj |
antibodies,with |
| R2544 |
T8252 |
T8251 |
prep |
against,antibodies |
| R2545 |
T8253 |
T8252 |
pobj |
p53,against |
| R2546 |
T8254 |
T8255 |
punct |
(,Novacastra |
| R2547 |
T8255 |
T8253 |
parataxis |
Novacastra,p53 |
| R2548 |
T8256 |
T8255 |
dep |
CM,Novacastra |
| R2549 |
T8257 |
T8256 |
punct |
-,CM |
| R2550 |
T8258 |
T8256 |
nummod |
5,CM |
| R2551 |
T8259 |
T8255 |
punct |
", ",Novacastra |
| R2552 |
T8260 |
T8255 |
punct |
),Novacastra |
| R2553 |
T8261 |
T8253 |
cc |
and,p53 |
| R2554 |
T8262 |
T8263 |
punct |
-,actin |
| R2555 |
T8263 |
T8253 |
conj |
actin,p53 |
| R2556 |
T8264 |
T8265 |
punct |
(,Sigma |
| R2557 |
T8265 |
T8263 |
parataxis |
Sigma,actin |
| R2558 |
T8266 |
T8265 |
punct |
),Sigma |
| R2559 |
T8267 |
T8212 |
punct |
.,incubated |
| R2560 |
T8269 |
T8270 |
amod |
Secondary,antibodies |
| R2561 |
T8270 |
T8271 |
nsubj |
antibodies,include |
| R2562 |
T8272 |
T8270 |
acl |
used,antibodies |
| R2563 |
T8273 |
T8274 |
npadvmod |
peroxidase,conjugated |
| R2564 |
T8274 |
T8276 |
amod |
conjugated,IgG |
| R2565 |
T8275 |
T8274 |
punct |
-,conjugated |
| R2566 |
T8276 |
T8271 |
dobj |
IgG,include |
| R2567 |
T8277 |
T8276 |
compound |
goat,IgG |
| R2568 |
T8278 |
T8276 |
compound |
anti-mouse,IgG |
| R2569 |
T8279 |
T8276 |
cc |
and,IgG |
| R2570 |
T8280 |
T8281 |
compound |
anti-rabbit,IgG |
| R2571 |
T8281 |
T8276 |
conj |
IgG,IgG |
| R2572 |
T8282 |
T8283 |
punct |
(,Pierce |
| R2573 |
T8283 |
T8281 |
parataxis |
Pierce,IgG |
| R2574 |
T8284 |
T8283 |
punct |
),Pierce |
| R2575 |
T8285 |
T8271 |
punct |
.,include |
| R2576 |
T8287 |
T8288 |
amod |
Probed,blots |
| R2577 |
T8288 |
T8289 |
nsubjpass |
blots,incubated |
| R2578 |
T8290 |
T8289 |
auxpass |
were,incubated |
| R2579 |
T8291 |
T8289 |
prep |
with,incubated |
| R2580 |
T8292 |
T8293 |
nmod |
Pierce,substrate |
| R2581 |
T8293 |
T8291 |
pobj |
substrate,with |
| R2582 |
T8294 |
T8295 |
nmod |
Supersignal,West |
| R2583 |
T8295 |
T8293 |
nmod |
West,substrate |
| R2584 |
T8296 |
T8293 |
nmod |
Pico,substrate |
| R2585 |
T8297 |
T8293 |
amod |
chemiluminescent,substrate |
| R2586 |
T8298 |
T8289 |
cc |
and,incubated |
| R2587 |
T8299 |
T8289 |
conj |
exposed,incubated |
| R2588 |
T8300 |
T8299 |
prep |
to,exposed |
| R2589 |
T8301 |
T8302 |
compound |
X-ray,films |
| R2590 |
T8302 |
T8300 |
pobj |
films,to |
| R2591 |
T8303 |
T8289 |
punct |
.,incubated |
