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PMC:1440874 / 26877-31668 JSONTXT 5 Projects

Annnotations TAB TSV DIC JSON TextAE

Id Subject Object Predicate Lexical cue
T5495 0-13 NN denotes Trophectoderm
T5496 14-21 NN denotes Failure
T5497 22-24 IN denotes in
T5498 25-33 JJ denotes Atrxnull
T5499 34-41 NNS denotes Embryos
T5500 41-266 sentence denotes Whole-mount observation of 8.5 dpc embryos revealed that, in contrast to the basically normal although delayed morphology of the embryo itself, the extraembryonic tissues of Atrxnull conceptuses appeared highly disorganised.
T5501 42-47 JJ denotes Whole
T5503 47-48 HYPH denotes -
T5502 48-53 NN denotes mount
T5504 54-65 NN denotes observation
T5506 66-68 IN denotes of
T5507 69-72 CD denotes 8.5
T5508 73-76 NN denotes dpc
T5509 77-84 NNS denotes embryos
T5505 85-93 VBD denotes revealed
T5510 94-98 IN denotes that
T5512 98-100 , denotes ,
T5513 100-102 IN denotes in
T5514 103-111 NN denotes contrast
T5515 112-114 IN denotes to
T5516 115-118 DT denotes the
T5518 119-128 RB denotes basically
T5519 129-135 JJ denotes normal
T5520 136-144 IN denotes although
T5521 145-152 VBN denotes delayed
T5517 153-163 NN denotes morphology
T5522 164-166 IN denotes of
T5523 167-170 DT denotes the
T5524 171-177 NN denotes embryo
T5525 178-184 PRP denotes itself
T5526 184-186 , denotes ,
T5527 186-189 DT denotes the
T5529 190-204 JJ denotes extraembryonic
T5528 205-212 NNS denotes tissues
T5530 213-215 IN denotes of
T5531 216-224 JJ denotes Atrxnull
T5532 225-236 NNS denotes conceptuses
T5511 237-245 VBD denotes appeared
T5533 246-252 RB denotes highly
T5534 253-265 JJ denotes disorganised
T5535 265-266 . denotes .
T5536 266-518 sentence denotes When embryos were removed from deciduas, the surrounding trophectoderm layer appeared dramatically reduced in Atrxnull embryos relative to wild-type littermates, and the underlying ectoplacental cone appeared reduced and abnormally shaped (Figure 7A).
T5537 267-271 WRB denotes When
T5539 272-279 NNS denotes embryos
T5540 280-284 VBD denotes were
T5538 285-292 VBN denotes removed
T5542 293-297 IN denotes from
T5543 298-306 NNS denotes deciduas
T5544 306-308 , denotes ,
T5545 308-311 DT denotes the
T5547 312-323 VBG denotes surrounding
T5548 324-337 NN denotes trophectoderm
T5546 338-343 NN denotes layer
T5541 344-352 VBD denotes appeared
T5549 353-365 RB denotes dramatically
T5550 366-373 JJ denotes reduced
T5551 374-376 IN denotes in
T5552 377-385 JJ denotes Atrxnull
T5553 386-393 NNS denotes embryos
T5554 394-402 JJ denotes relative
T5555 403-405 IN denotes to
T5556 406-410 JJ denotes wild
T5558 410-411 HYPH denotes -
T5557 411-415 NN denotes type
T5559 416-427 NNS denotes littermates
T5560 427-429 , denotes ,
T5561 429-432 CC denotes and
T5562 433-436 DT denotes the
T5564 437-447 VBG denotes underlying
T5565 448-461 JJ denotes ectoplacental
T5563 462-466 NN denotes cone
T5566 467-475 VBD denotes appeared
T5567 476-483 JJ denotes reduced
T5568 484-487 CC denotes and
T5569 488-498 RB denotes abnormally
T5570 499-505 VBN denotes shaped
T5571 506-507 -LRB- denotes (
T5573 507-513 NN denotes Figure
T5572 514-516 NN denotes 7A
T5574 516-517 -RRB- denotes )
T5575 517-518 . denotes .
T5576 518-713 sentence denotes Vacated deciduas surrounding 8.5 dpc wild-type and Atrxnull embryos were bisected and analysed by WMISH for expression of placental lactogen-1 (Pl-1), a marker of terminally differentiated TGCs.
T5577 519-526 VBN denotes Vacated
T5578 527-535 NNS denotes deciduas
T5580 536-547 VBG denotes surrounding
T5581 548-551 CD denotes 8.5
T5582 552-555 NN denotes dpc
T5584 556-560 JJ denotes wild
T5586 560-561 HYPH denotes -
T5585 561-565 NN denotes type
T5587 566-569 CC denotes and
T5588 570-578 JJ denotes Atrxnull
T5583 579-586 NNS denotes embryos
T5589 587-591 VBD denotes were
T5579 592-600 VBN denotes bisected
T5590 601-604 CC denotes and
T5591 605-613 VBN denotes analysed
T5592 614-616 IN denotes by
T5593 617-622 NN denotes WMISH
T5594 623-626 IN denotes for
T5595 627-637 NN denotes expression
T5596 638-640 IN denotes of
T5597 641-650 JJ denotes placental
T5598 651-659 NN denotes lactogen
T5599 659-660 HYPH denotes -
T5600 660-661 CD denotes 1
T5601 662-663 -LRB- denotes (
T5602 663-665 NN denotes Pl
T5603 665-666 HYPH denotes -
T5604 666-667 CD denotes 1
T5605 667-668 -RRB- denotes )
T5606 668-670 , denotes ,
T5607 670-671 DT denotes a
T5608 672-678 NN denotes marker
T5609 679-681 IN denotes of
T5610 682-692 RB denotes terminally
T5611 693-707 VBN denotes differentiated
T5612 708-712 NNS denotes TGCs
T5613 712-713 . denotes .
T5614 713-901 sentence denotes The number of Pl-1-expressing cells attached to the decidual wall after removal of the embryo is an indication of the density of trophoblast cells surrounding each implantation site [17].
T5615 714-717 DT denotes The
T5616 718-724 NN denotes number
T5618 725-727 IN denotes of
T5619 728-730 NN denotes Pl
T5621 730-731 HYPH denotes -
T5622 731-732 CD denotes 1
T5623 732-733 HYPH denotes -
T5620 733-743 VBG denotes expressing
T5624 744-749 NNS denotes cells
T5625 750-758 VBN denotes attached
T5626 759-761 IN denotes to
T5627 762-765 DT denotes the
T5629 766-774 JJ denotes decidual
T5628 775-779 NN denotes wall
T5630 780-785 IN denotes after
T5631 786-793 NN denotes removal
T5632 794-796 IN denotes of
T5633 797-800 DT denotes the
T5634 801-807 NN denotes embryo
T5617 808-810 VBZ denotes is
T5635 811-813 DT denotes an
T5636 814-824 NN denotes indication
T5637 825-827 IN denotes of
T5638 828-831 DT denotes the
T5639 832-839 NN denotes density
T5640 840-842 IN denotes of
T5641 843-854 NN denotes trophoblast
T5642 855-860 NNS denotes cells
T5643 861-872 VBG denotes surrounding
T5644 873-877 DT denotes each
T5646 878-890 NN denotes implantation
T5645 891-895 NN denotes site
T5647 896-897 -LRB- denotes [
T5648 897-899 CD denotes 17
T5649 899-900 -RRB- denotes ]
T5650 900-901 . denotes .
T5651 901-1260 sentence denotes We found that the population of Pl-1-expressing cells was depleted in the decidual implantation sites containing Atrxnull embryos relative to those containing wild-type littermates (Figure 7B); this was also apparent at 7.5 dpc, as determined by immunohistochemical staining of paraffin sections of embryos in deciduas with an anti-Pl-1 antibody (Figure 7C).
T5652 902-904 PRP denotes We
T5653 905-910 VBD denotes found
T5655 911-915 IN denotes that
T5657 916-919 DT denotes the
T5658 920-930 NN denotes population
T5659 931-933 IN denotes of
T5660 934-936 NN denotes Pl
T5662 936-937 HYPH denotes -
T5663 937-938 CD denotes 1
T5664 938-939 HYPH denotes -
T5661 939-949 VBG denotes expressing
T5665 950-955 NNS denotes cells
T5666 956-959 VBD denotes was
T5656 960-968 VBN denotes depleted
T5667 969-971 IN denotes in
T5668 972-975 DT denotes the
T5670 976-984 JJ denotes decidual
T5671 985-997 NN denotes implantation
T5669 998-1003 NNS denotes sites
T5672 1004-1014 VBG denotes containing
T5673 1015-1023 JJ denotes Atrxnull
T5674 1024-1031 NNS denotes embryos
T5675 1032-1040 JJ denotes relative
T5676 1041-1043 IN denotes to
T5677 1044-1049 DT denotes those
T5678 1050-1060 VBG denotes containing
T5679 1061-1065 JJ denotes wild
T5681 1065-1066 HYPH denotes -
T5680 1066-1070 NN denotes type
T5682 1071-1082 NNS denotes littermates
T5683 1083-1084 -LRB- denotes (
T5685 1084-1090 NN denotes Figure
T5684 1091-1093 NN denotes 7B
T5686 1093-1094 -RRB- denotes )
T5687 1094-1095 : denotes ;
T5688 1096-1100 DT denotes this
T5654 1101-1104 VBD denotes was
T5689 1105-1109 RB denotes also
T5690 1110-1118 JJ denotes apparent
T5691 1119-1121 IN denotes at
T5692 1122-1125 CD denotes 7.5
T5693 1126-1129 NN denotes dpc
T5694 1129-1131 , denotes ,
T5695 1131-1133 IN denotes as
T5696 1134-1144 VBN denotes determined
T5697 1145-1147 IN denotes by
T5698 1148-1167 JJ denotes immunohistochemical
T5699 1168-1176 NN denotes staining
T5700 1177-1179 IN denotes of
T5701 1180-1188 NN denotes paraffin
T5702 1189-1197 NNS denotes sections
T5703 1198-1200 IN denotes of
T5704 1201-1208 NNS denotes embryos
T5705 1209-1211 IN denotes in
T5706 1212-1220 NNS denotes deciduas
T5707 1221-1225 IN denotes with
T5708 1226-1228 DT denotes an
T5710 1229-1236 JJ denotes anti-Pl
T5712 1236-1237 HYPH denotes -
T5711 1237-1238 CD denotes 1
T5709 1239-1247 NN denotes antibody
T5713 1248-1249 -LRB- denotes (
T5715 1249-1255 NN denotes Figure
T5714 1256-1258 NN denotes 7C
T5716 1258-1259 -RRB- denotes )
T5717 1259-1260 . denotes .
T5718 1260-1431 sentence denotes A TGC deficiency in the absence of Atrx is consistent with the observation that Atrx is highly expressed in giant cells surrounding wild-type 7.5 dpc embryos (Figure 5E).
T5719 1261-1262 DT denotes A
T5721 1263-1266 NN denotes TGC
T5720 1267-1277 NN denotes deficiency
T5723 1278-1280 IN denotes in
T5724 1281-1284 DT denotes the
T5725 1285-1292 NN denotes absence
T5726 1293-1295 IN denotes of
T5727 1296-1300 NN denotes Atrx
T5722 1301-1303 VBZ denotes is
T5728 1304-1314 JJ denotes consistent
T5729 1315-1319 IN denotes with
T5730 1320-1323 DT denotes the
T5731 1324-1335 NN denotes observation
T5732 1336-1340 IN denotes that
T5734 1341-1345 NN denotes Atrx
T5733 1346-1348 VBZ denotes is
T5735 1349-1355 RB denotes highly
T5736 1356-1365 JJ denotes expressed
T5737 1366-1368 IN denotes in
T5738 1369-1374 JJ denotes giant
T5739 1375-1380 NNS denotes cells
T5740 1381-1392 VBG denotes surrounding
T5741 1393-1397 JJ denotes wild
T5743 1397-1398 HYPH denotes -
T5742 1398-1402 NN denotes type
T5745 1403-1406 CD denotes 7.5
T5746 1407-1410 NN denotes dpc
T5744 1411-1418 NNS denotes embryos
T5747 1419-1420 -LRB- denotes (
T5749 1420-1426 NN denotes Figure
T5748 1427-1429 NN denotes 5E
T5750 1429-1430 -RRB- denotes )
T5751 1430-1431 . denotes .
T5752 1431-3573 sentence denotes Figure 7 Trophectoderm Defect in Atrxnull Embryos (A) 8.5 dpc embryos were dissected from surrounding decidual tissue and observed in whole mount. The genotype of each (indicated above) was determined by PCR using DNA extracted from whole embryos after photography. In the left image, the wild-type female (three-somite stage, left) is surrounded by trophoblast (t) while the trophoblast component surrounding the Atrxnull males (at headfold/presomite [middle] and two-somite stages [right], respectively) is severely depleted. In the right image, the trophoblast has been dissected away from the embryonic region of the wild-type embryo, to reveal the small, abnormally shaped ectoplacental cone (epc) of the mutant littermates. (B) WMISH to detect expression of Pl-1 (a marker of TGCs) at the implantation sites in vacated deciduas that had contained 8.5 dpc wild-type (Atrx WT/WT) or Atrxnull (Atrx Δ18Δneo/Y) embryos. The genotype was determined by PCR using DNA extracted from whole embryos. TGCs are stained with Pl-1. (C) Paraffin sections of wild-type or Atrxnull 7.5 dpc embryos (dissected in their deciduas) were stained with an anti-Pl-1 antibody. The presence or absence of Atrx in each embryo was determined by staining adjacent sections with the anti-ATRX antibody (H-300) as in Figure 5 (unpublished data). (D) Examples of 5-d blastocyst outgrowth cultures. Extensive trophoblast outgrowing from the inner cell mass (icm) was observed in all genotypes. The Atrx genotype and sex of the blastocyst indicated were determined by PCR. To investigate whether the trophoblast defect was restricted to the production of secondary TGCs (produced by diploid precursors in the ectoplacental cone and derived originally from the polar trophectoderm overlying the inner cell mass of the blastocyst) or also affected the production of primary TGCs (resulting from differentiation of the mural trophectoderm of the blastocyst), blastocysts (3.5 dpc) from crosses between Atrx WT/flox females and GATA1-Cre homozygous transgenic males (Atrx WT/Y;GATA1-Cre +/+) were cultured in vitro for 5 d to monitor outgrowth of the primary trophoblast.
T5753 2979-2981 TO denotes To
T5754 2982-2993 VB denotes investigate
T5756 2994-3001 IN denotes whether
T5758 3002-3005 DT denotes the
T5760 3006-3017 NN denotes trophoblast
T5759 3018-3024 NN denotes defect
T5761 3025-3028 VBD denotes was
T5757 3029-3039 VBN denotes restricted
T5762 3040-3042 IN denotes to
T5763 3043-3046 DT denotes the
T5764 3047-3057 NN denotes production
T5765 3058-3060 IN denotes of
T5766 3061-3070 JJ denotes secondary
T5767 3071-3075 NNS denotes TGCs
T5768 3076-3077 -LRB- denotes (
T5769 3077-3085 VBN denotes produced
T5770 3086-3088 IN denotes by
T5771 3089-3096 NN denotes diploid
T5772 3097-3107 NNS denotes precursors
T5773 3108-3110 IN denotes in
T5774 3111-3114 DT denotes the
T5776 3115-3128 JJ denotes ectoplacental
T5775 3129-3133 NN denotes cone
T5777 3134-3137 CC denotes and
T5778 3138-3145 VBN denotes derived
T5779 3146-3156 RB denotes originally
T5780 3157-3161 IN denotes from
T5781 3162-3165 DT denotes the
T5783 3166-3171 JJ denotes polar
T5782 3172-3185 NN denotes trophectoderm
T5784 3186-3195 VBG denotes overlying
T5785 3196-3199 DT denotes the
T5787 3200-3205 JJ denotes inner
T5788 3206-3210 NN denotes cell
T5786 3211-3215 NN denotes mass
T5789 3216-3218 IN denotes of
T5790 3219-3222 DT denotes the
T5791 3223-3233 NN denotes blastocyst
T5792 3233-3234 -RRB- denotes )
T5793 3235-3237 CC denotes or
T5794 3238-3242 RB denotes also
T5795 3243-3251 VBD denotes affected
T5796 3252-3255 DT denotes the
T5797 3256-3266 NN denotes production
T5798 3267-3269 IN denotes of
T5799 3270-3277 JJ denotes primary
T5800 3278-3282 NNS denotes TGCs
T5801 3283-3284 -LRB- denotes (
T5802 3284-3293 VBG denotes resulting
T5803 3294-3298 IN denotes from
T5804 3299-3314 NN denotes differentiation
T5805 3315-3317 IN denotes of
T5806 3318-3321 DT denotes the
T5808 3322-3327 JJ denotes mural
T5807 3328-3341 NN denotes trophectoderm
T5809 3342-3344 IN denotes of
T5810 3345-3348 DT denotes the
T5811 3349-3359 NN denotes blastocyst
T5812 3359-3360 -RRB- denotes )
T5813 3360-3362 , denotes ,
T5814 3362-3373 NNS denotes blastocysts
T5815 3374-3375 -LRB- denotes (
T5817 3375-3378 CD denotes 3.5
T5816 3379-3382 NN denotes dpc
T5818 3382-3383 -RRB- denotes )
T5819 3384-3388 IN denotes from
T5820 3389-3396 NNS denotes crosses
T5821 3397-3404 IN denotes between
T5822 3405-3412 NN denotes Atrx WT
T5824 3412-3413 HYPH denotes /
T5823 3413-3417 NN denotes flox
T5825 3418-3425 NNS denotes females
T5826 3426-3429 CC denotes and
T5827 3430-3435 NN denotes GATA1
T5829 3435-3436 HYPH denotes -
T5828 3436-3439 NN denotes Cre
T5830 3440-3450 JJ denotes homozygous
T5832 3451-3461 JJ denotes transgenic
T5831 3462-3467 NNS denotes males
T5833 3468-3469 -LRB- denotes (
T5834 3469-3476 NN denotes Atrx WT
T5836 3476-3477 HYPH denotes /
T5835 3477-3478 NN denotes Y
T5837 3478-3479 : denotes ;
T5838 3479-3484 NN denotes GATA1
T5840 3484-3485 HYPH denotes -
T5839 3485-3488 NN denotes Cre
T5841 3489-3490 SYM denotes +
T5842 3490-3491 HYPH denotes /
T5843 3491-3492 SYM denotes +
T5844 3492-3493 -RRB- denotes )
T5845 3494-3498 VBD denotes were
T5755 3499-3507 VBN denotes cultured
T5846 3508-3510 FW denotes in
T5847 3511-3516 FW denotes vitro
T5848 3517-3520 IN denotes for
T5849 3521-3522 CD denotes 5
T5850 3523-3524 NNS denotes d
T5851 3525-3527 TO denotes to
T5852 3528-3535 VB denotes monitor
T5853 3536-3545 NN denotes outgrowth
T5854 3546-3548 IN denotes of
T5855 3549-3552 DT denotes the
T5857 3553-3560 JJ denotes primary
T5856 3561-3572 NN denotes trophoblast
T5858 3572-3573 . denotes .
T5859 3573-3748 sentence denotes After 5 d, individual blastocyst cultures were scored for the extent of primary trophoblast outgrowth, and the Atrx genotype and sex of the blastocyst were determined by PCR.
T5860 3574-3579 IN denotes After
T5862 3580-3581 CD denotes 5
T5863 3582-3583 NNS denotes d
T5864 3583-3585 , denotes ,
T5865 3585-3595 JJ denotes individual
T5867 3596-3606 NN denotes blastocyst
T5866 3607-3615 NNS denotes cultures
T5868 3616-3620 VBD denotes were
T5861 3621-3627 VBN denotes scored
T5869 3628-3631 IN denotes for
T5870 3632-3635 DT denotes the
T5871 3636-3642 NN denotes extent
T5872 3643-3645 IN denotes of
T5873 3646-3653 JJ denotes primary
T5875 3654-3665 NN denotes trophoblast
T5874 3666-3675 NN denotes outgrowth
T5876 3675-3677 , denotes ,
T5877 3677-3680 CC denotes and
T5878 3681-3684 DT denotes the
T5880 3685-3689 NN denotes Atrx
T5879 3690-3698 NN denotes genotype
T5882 3699-3702 CC denotes and
T5883 3703-3706 NN denotes sex
T5884 3707-3709 IN denotes of
T5885 3710-3713 DT denotes the
T5886 3714-3724 NN denotes blastocyst
T5887 3725-3729 VBD denotes were
T5881 3730-3740 VBN denotes determined
T5888 3741-3743 IN denotes by
T5889 3744-3747 NN denotes PCR
T5890 3747-3748 . denotes .
T5891 3748-3921 sentence denotes Most blastocysts hatched from the zona pellucida within 24 h, and trophoblast cells spreading out from the inner cell mass could usually be detected within 48 h of culture.
T5892 3749-3753 JJS denotes Most
T5893 3754-3765 NNS denotes blastocysts
T5894 3766-3773 VBN denotes hatched
T5895 3774-3778 IN denotes from
T5896 3779-3782 DT denotes the
T5898 3783-3787 NN denotes zona
T5897 3788-3797 NN denotes pellucida
T5899 3798-3804 IN denotes within
T5900 3805-3807 CD denotes 24
T5901 3808-3809 NNS denotes h
T5902 3809-3811 , denotes ,
T5903 3811-3814 CC denotes and
T5904 3815-3826 NN denotes trophoblast
T5905 3827-3832 NNS denotes cells
T5907 3833-3842 VBG denotes spreading
T5908 3843-3846 RP denotes out
T5909 3847-3851 IN denotes from
T5910 3852-3855 DT denotes the
T5912 3856-3861 JJ denotes inner
T5913 3862-3866 NN denotes cell
T5911 3867-3871 NN denotes mass
T5914 3872-3877 MD denotes could
T5915 3878-3885 RB denotes usually
T5916 3886-3888 VB denotes be
T5906 3889-3897 VBN denotes detected
T5917 3898-3904 IN denotes within
T5918 3905-3907 CD denotes 48
T5919 3908-3909 NNS denotes h
T5920 3910-3912 IN denotes of
T5921 3913-3920 NN denotes culture
T5922 3920-3921 . denotes .
T5923 3921-4284 sentence denotes No difference was observed in the rate or extent of trophoblast outgrowth over 5 d of culture between Atrxnull blastocysts (Atrx null/Y, n = 6) and blastocysts bearing an Atrx WT allele (Atrx WT/WT, n = 6; Atrx WT/null, n = 6; Atrx WT/Y, n = 6) (examples shown in Figure 7D), suggesting that the defect specifically involves the secondary giant cell compartment.
T5924 3922-3924 DT denotes No
T5925 3925-3935 NN denotes difference
T5927 3936-3939 VBD denotes was
T5926 3940-3948 VBN denotes observed
T5928 3949-3951 IN denotes in
T5929 3952-3955 DT denotes the
T5930 3956-3960 NN denotes rate
T5931 3961-3963 CC denotes or
T5932 3964-3970 NN denotes extent
T5933 3971-3973 IN denotes of
T5934 3974-3985 NN denotes trophoblast
T5935 3986-3995 NN denotes outgrowth
T5936 3996-4000 IN denotes over
T5937 4001-4002 CD denotes 5
T5938 4003-4004 NNS denotes d
T5939 4005-4007 IN denotes of
T5940 4008-4015 NN denotes culture
T5941 4016-4023 IN denotes between
T5942 4024-4032 JJ denotes Atrxnull
T5943 4033-4044 NNS denotes blastocysts
T5944 4045-4046 -LRB- denotes (
T5946 4046-4055 JJ denotes Atrx null
T5947 4055-4056 HYPH denotes /
T5945 4056-4057 NN denotes Y
T5948 4057-4059 , denotes ,
T5949 4059-4060 NN denotes n
T5951 4061-4062 SYM denotes =
T5950 4063-4064 CD denotes 6
T5952 4064-4065 -RRB- denotes )
T5953 4066-4069 CC denotes and
T5954 4070-4081 NNS denotes blastocysts
T5955 4082-4089 VBG denotes bearing
T5956 4090-4092 DT denotes an
T5958 4093-4100 NN denotes Atrx WT
T5957 4101-4107 NN denotes allele
T5959 4108-4109 -LRB- denotes (
T5961 4109-4116 NN denotes Atrx WT
T5963 4116-4117 HYPH denotes /
T5962 4117-4119 NN denotes WT
T5965 4119-4121 , denotes ,
T5966 4121-4122 NN denotes n
T5967 4123-4124 SYM denotes =
T5964 4125-4126 CD denotes 6
T5968 4126-4127 : denotes ;
T5969 4128-4135 NN denotes Atrx WT
T5971 4135-4136 HYPH denotes /
T5970 4136-4140 NN denotes null
T5973 4140-4142 , denotes ,
T5974 4142-4143 NN denotes n
T5975 4144-4145 SYM denotes =
T5972 4146-4147 CD denotes 6
T5976 4147-4148 : denotes ;
T5977 4149-4156 NN denotes Atrx WT
T5979 4156-4157 HYPH denotes /
T5978 4157-4158 NN denotes Y
T5980 4158-4160 , denotes ,
T5981 4160-4161 NN denotes n
T5982 4162-4163 SYM denotes =
T5960 4164-4165 CD denotes 6
T5983 4165-4166 -RRB- denotes )
T5984 4167-4168 -LRB- denotes (
T5986 4168-4176 NNS denotes examples
T5985 4177-4182 VBN denotes shown
T5987 4183-4185 IN denotes in
T5988 4186-4192 NN denotes Figure
T5989 4193-4195 NN denotes 7D
T5990 4195-4196 -RRB- denotes )
T5991 4196-4198 , denotes ,
T5992 4198-4208 VBG denotes suggesting
T5993 4209-4213 IN denotes that
T5995 4214-4217 DT denotes the
T5996 4218-4224 NN denotes defect
T5997 4225-4237 RB denotes specifically
T5994 4238-4246 VBZ denotes involves
T5998 4247-4250 DT denotes the
T6000 4251-4260 JJ denotes secondary
T6001 4261-4266 JJ denotes giant
T6002 4267-4271 NN denotes cell
T5999 4272-4283 NN denotes compartment
T6003 4283-4284 . denotes .
T6004 4284-4400 sentence denotes This is consistent with the observation that Atrxnull conceptuses implant successfully and survive to gastrulation.
T6005 4285-4289 DT denotes This
T6006 4290-4292 VBZ denotes is
T6007 4293-4303 JJ denotes consistent
T6008 4304-4308 IN denotes with
T6009 4309-4312 DT denotes the
T6010 4313-4324 NN denotes observation
T6011 4325-4329 IN denotes that
T6013 4330-4338 JJ denotes Atrxnull
T6014 4339-4350 NNS denotes conceptuses
T6012 4351-4358 VBP denotes implant
T6015 4359-4371 RB denotes successfully
T6016 4372-4375 CC denotes and
T6017 4376-4383 VBP denotes survive
T6018 4384-4386 IN denotes to
T6019 4387-4399 NN denotes gastrulation
T6020 4399-4400 . denotes .
T6021 4400-4546 sentence denotes Taken together, these data suggest that loss of Atrx results in a defect in formation of the secondary trophoblast that is apparent from 7.5 dpc.
T6022 4401-4406 VBN denotes Taken
T6024 4407-4415 RB denotes together
T6025 4415-4417 , denotes ,
T6026 4417-4422 DT denotes these
T6027 4423-4427 NNS denotes data
T6023 4428-4435 VBP denotes suggest
T6028 4436-4440 IN denotes that
T6030 4441-4445 NN denotes loss
T6031 4446-4448 IN denotes of
T6032 4449-4453 NN denotes Atrx
T6029 4454-4461 VBZ denotes results
T6033 4462-4464 IN denotes in
T6034 4465-4466 DT denotes a
T6035 4467-4473 NN denotes defect
T6036 4474-4476 IN denotes in
T6037 4477-4486 NN denotes formation
T6038 4487-4489 IN denotes of
T6039 4490-4493 DT denotes the
T6041 4494-4503 JJ denotes secondary
T6040 4504-4515 NN denotes trophoblast
T6042 4516-4520 WDT denotes that
T6043 4521-4523 VBZ denotes is
T6044 4524-4532 JJ denotes apparent
T6045 4533-4537 IN denotes from
T6046 4538-4541 CD denotes 7.5
T6047 4542-4545 NN denotes dpc
T6048 4545-4546 . denotes .
T6049 4546-4791 sentence denotes Despite initiating normal organisation in the embryo proper, Atrxnull conceptuses exhibit a proliferative defect by 7.5 dpc and die by around 9.5 dpc, probably due to a nutritional deficit resulting from failure to develop a normal trophoblast.
T6050 4547-4554 IN denotes Despite
T6052 4555-4565 VBG denotes initiating
T6053 4566-4572 JJ denotes normal
T6054 4573-4585 NN denotes organisation
T6055 4586-4588 IN denotes in
T6056 4589-4592 DT denotes the
T6057 4593-4599 NN denotes embryo
T6058 4600-4606 JJ denotes proper
T6059 4606-4608 , denotes ,
T6060 4608-4616 JJ denotes Atrxnull
T6061 4617-4628 NNS denotes conceptuses
T6051 4629-4636 VBP denotes exhibit
T6062 4637-4638 DT denotes a
T6064 4639-4652 JJ denotes proliferative
T6063 4653-4659 NN denotes defect
T6065 4660-4662 IN denotes by
T6066 4663-4666 CD denotes 7.5
T6067 4667-4670 NN denotes dpc
T6068 4671-4674 CC denotes and
T6069 4675-4678 VBP denotes die
T6070 4679-4681 IN denotes by
T6071 4682-4688 IN denotes around
T6072 4689-4692 CD denotes 9.5
T6073 4693-4696 NN denotes dpc
T6074 4696-4698 , denotes ,
T6075 4698-4706 RB denotes probably
T6076 4707-4710 IN denotes due
T6077 4711-4713 IN denotes to
T6078 4714-4715 DT denotes a
T6080 4716-4727 JJ denotes nutritional
T6079 4728-4735 NN denotes deficit
T6081 4736-4745 VBG denotes resulting
T6082 4746-4750 IN denotes from
T6083 4751-4758 NN denotes failure
T6084 4759-4761 TO denotes to
T6085 4762-4769 VB denotes develop
T6086 4770-4771 DT denotes a
T6088 4772-4778 JJ denotes normal
T6087 4779-4790 NN denotes trophoblast
T6089 4790-4791 . denotes .
R3285 T5495 T5496 compound Trophectoderm,Failure
R3286 T5497 T5496 prep in,Failure
R3287 T5498 T5499 amod Atrxnull,Embryos
R3288 T5499 T5497 pobj Embryos,in
R3289 T5501 T5502 amod Whole,mount
R3290 T5502 T5504 compound mount,observation
R3291 T5503 T5502 punct -,mount
R3292 T5504 T5505 nsubj observation,revealed
R3293 T5506 T5504 prep of,observation
R3294 T5507 T5508 nummod 8.5,dpc
R3295 T5508 T5509 compound dpc,embryos
R3296 T5509 T5506 pobj embryos,of
R3297 T5510 T5511 mark that,appeared
R3298 T5511 T5505 ccomp appeared,revealed
R3299 T5512 T5511 punct ", ",appeared
R3300 T5513 T5511 prep in,appeared
R3301 T5514 T5513 pobj contrast,in
R3302 T5515 T5514 prep to,contrast
R3303 T5516 T5517 det the,morphology
R3304 T5517 T5515 pobj morphology,to
R3305 T5518 T5519 advmod basically,normal
R3306 T5519 T5517 amod normal,morphology
R3307 T5520 T5521 mark although,delayed
R3308 T5521 T5519 parataxis delayed,normal
R3309 T5522 T5517 prep of,morphology
R3310 T5523 T5524 det the,embryo
R3311 T5524 T5522 pobj embryo,of
R3312 T5525 T5524 appos itself,embryo
R3313 T5526 T5511 punct ", ",appeared
R3314 T5527 T5528 det the,tissues
R3315 T5528 T5511 nsubj tissues,appeared
R3316 T5529 T5528 amod extraembryonic,tissues
R3317 T5530 T5528 prep of,tissues
R3318 T5531 T5532 amod Atrxnull,conceptuses
R3319 T5532 T5530 pobj conceptuses,of
R3320 T5533 T5534 advmod highly,disorganised
R3321 T5534 T5511 oprd disorganised,appeared
R3322 T5535 T5505 punct .,revealed
R3323 T5537 T5538 advmod When,removed
R3324 T5538 T5541 advcl removed,appeared
R3325 T5539 T5538 nsubjpass embryos,removed
R3326 T5540 T5538 auxpass were,removed
R3327 T5542 T5538 prep from,removed
R3328 T5543 T5542 pobj deciduas,from
R3329 T5544 T5541 punct ", ",appeared
R3330 T5545 T5546 det the,layer
R3331 T5546 T5541 nsubj layer,appeared
R3332 T5547 T5546 amod surrounding,layer
R3333 T5548 T5546 compound trophectoderm,layer
R3334 T5549 T5550 advmod dramatically,reduced
R3335 T5550 T5541 oprd reduced,appeared
R3336 T5551 T5541 prep in,appeared
R3337 T5552 T5553 amod Atrxnull,embryos
R3338 T5553 T5551 pobj embryos,in
R3339 T5554 T5541 advcl relative,appeared
R3340 T5555 T5554 prep to,relative
R3341 T5556 T5557 amod wild,type
R3342 T5557 T5559 compound type,littermates
R3343 T5558 T5557 punct -,type
R3344 T5559 T5555 pobj littermates,to
R3345 T5560 T5541 punct ", ",appeared
R3346 T5561 T5541 cc and,appeared
R3347 T5562 T5563 det the,cone
R3348 T5563 T5566 nsubj cone,appeared
R3349 T5564 T5563 amod underlying,cone
R3350 T5565 T5563 amod ectoplacental,cone
R3351 T5566 T5541 conj appeared,appeared
R3352 T5567 T5566 oprd reduced,appeared
R3353 T5568 T5567 cc and,reduced
R3354 T5569 T5570 advmod abnormally,shaped
R3355 T5570 T5567 conj shaped,reduced
R3356 T5571 T5572 punct (,7A
R3357 T5572 T5566 parataxis 7A,appeared
R3358 T5573 T5572 compound Figure,7A
R3359 T5574 T5572 punct ),7A
R3360 T5575 T5566 punct .,appeared
R3361 T5577 T5578 amod Vacated,deciduas
R3362 T5578 T5579 nsubjpass deciduas,bisected
R3363 T5580 T5578 acl surrounding,deciduas
R3364 T5581 T5582 nummod 8.5,dpc
R3365 T5582 T5583 nmod dpc,embryos
R3366 T5583 T5580 dobj embryos,surrounding
R3367 T5584 T5585 amod wild,type
R3368 T5585 T5583 nmod type,embryos
R3369 T5586 T5585 punct -,type
R3370 T5587 T5585 cc and,type
R3371 T5588 T5585 conj Atrxnull,type
R3372 T5589 T5579 auxpass were,bisected
R3373 T5590 T5579 cc and,bisected
R3374 T5591 T5579 conj analysed,bisected
R3375 T5592 T5591 prep by,analysed
R3376 T5593 T5592 pobj WMISH,by
R3377 T5594 T5591 prep for,analysed
R3378 T5595 T5594 pobj expression,for
R3379 T5596 T5595 prep of,expression
R3380 T5597 T5598 amod placental,lactogen
R3381 T5598 T5596 pobj lactogen,of
R3382 T5599 T5598 punct -,lactogen
R3383 T5600 T5598 nummod 1,lactogen
R3384 T5601 T5598 punct (,lactogen
R3385 T5602 T5598 appos Pl,lactogen
R3386 T5603 T5602 punct -,Pl
R3387 T5604 T5602 nummod 1,Pl
R3388 T5605 T5598 punct ),lactogen
R3389 T5606 T5598 punct ", ",lactogen
R3390 T5607 T5608 det a,marker
R3391 T5608 T5598 appos marker,lactogen
R3392 T5609 T5608 prep of,marker
R3393 T5610 T5611 advmod terminally,differentiated
R3394 T5611 T5612 amod differentiated,TGCs
R3395 T5612 T5609 pobj TGCs,of
R3396 T5613 T5579 punct .,bisected
R3397 T5615 T5616 det The,number
R3398 T5616 T5617 nsubj number,is
R3399 T5618 T5616 prep of,number
R3400 T5619 T5620 npadvmod Pl,expressing
R3401 T5620 T5624 amod expressing,cells
R3402 T5621 T5619 punct -,Pl
R3403 T5622 T5619 nummod 1,Pl
R3404 T5623 T5620 punct -,expressing
R3405 T5624 T5618 pobj cells,of
R3406 T5625 T5624 acl attached,cells
R3407 T5626 T5625 prep to,attached
R3408 T5627 T5628 det the,wall
R3409 T5628 T5626 pobj wall,to
R3410 T5629 T5628 amod decidual,wall
R3411 T5630 T5625 prep after,attached
R3412 T5631 T5630 pobj removal,after
R3413 T5632 T5631 prep of,removal
R3414 T5633 T5634 det the,embryo
R3415 T5634 T5632 pobj embryo,of
R3416 T5635 T5636 det an,indication
R3417 T5636 T5617 attr indication,is
R3418 T5637 T5636 prep of,indication
R3419 T5638 T5639 det the,density
R3420 T5639 T5637 pobj density,of
R3421 T5640 T5639 prep of,density
R3422 T5641 T5642 compound trophoblast,cells
R3423 T5642 T5640 pobj cells,of
R3424 T5643 T5642 acl surrounding,cells
R3425 T5644 T5645 det each,site
R3426 T5645 T5643 dobj site,surrounding
R3427 T5646 T5645 compound implantation,site
R3428 T5647 T5648 punct [,17
R3429 T5648 T5617 parataxis 17,is
R3430 T5649 T5648 punct ],17
R3431 T5650 T5617 punct .,is
R3432 T5652 T5653 nsubj We,found
R3433 T5653 T5654 ccomp found,was
R3434 T5655 T5656 mark that,depleted
R3435 T5656 T5653 ccomp depleted,found
R3436 T5657 T5658 det the,population
R3437 T5658 T5656 nsubjpass population,depleted
R3438 T5659 T5658 prep of,population
R3439 T5660 T5661 npadvmod Pl,expressing
R3440 T5661 T5665 amod expressing,cells
R3441 T5662 T5660 punct -,Pl
R3442 T5663 T5660 nummod 1,Pl
R3443 T5664 T5661 punct -,expressing
R3444 T5665 T5659 pobj cells,of
R3445 T5666 T5656 auxpass was,depleted
R3446 T5667 T5656 prep in,depleted
R3447 T5668 T5669 det the,sites
R3448 T5669 T5667 pobj sites,in
R3449 T5670 T5669 amod decidual,sites
R3450 T5671 T5669 compound implantation,sites
R3451 T5672 T5669 acl containing,sites
R3452 T5673 T5674 amod Atrxnull,embryos
R3453 T5674 T5672 dobj embryos,containing
R3454 T5675 T5656 advcl relative,depleted
R3455 T5676 T5675 prep to,relative
R3456 T5677 T5676 pobj those,to
R3457 T5678 T5677 acl containing,those
R3458 T5679 T5680 amod wild,type
R3459 T5680 T5682 compound type,littermates
R3460 T5681 T5680 punct -,type
R3461 T5682 T5678 dobj littermates,containing
R3462 T5683 T5684 punct (,7B
R3463 T5684 T5653 parataxis 7B,found
R3464 T5685 T5684 compound Figure,7B
R3465 T5686 T5684 punct ),7B
R3466 T5687 T5654 punct ;,was
R3467 T5688 T5654 nsubj this,was
R3468 T5689 T5654 advmod also,was
R3469 T5690 T5654 acomp apparent,was
R3470 T5691 T5654 prep at,was
R3471 T5692 T5693 nummod 7.5,dpc
R3472 T5693 T5691 pobj dpc,at
R3473 T5694 T5654 punct ", ",was
R3474 T5695 T5696 mark as,determined
R3475 T5696 T5654 advcl determined,was
R3476 T5697 T5696 prep by,determined
R3477 T5698 T5699 amod immunohistochemical,staining
R3478 T5699 T5697 pobj staining,by
R3479 T5700 T5699 prep of,staining
R3480 T5701 T5702 compound paraffin,sections
R3481 T5702 T5700 pobj sections,of
R3482 T5703 T5702 prep of,sections
R3483 T5704 T5703 pobj embryos,of
R3484 T5705 T5704 prep in,embryos
R3485 T5706 T5705 pobj deciduas,in
R3486 T5707 T5699 prep with,staining
R3487 T5708 T5709 det an,antibody
R3488 T5709 T5707 pobj antibody,with
R3489 T5710 T5711 amod anti-Pl,1
R3490 T5711 T5709 nummod 1,antibody
R3491 T5712 T5711 punct -,1
R3492 T5713 T5714 punct (,7C
R3493 T5714 T5654 parataxis 7C,was
R3494 T5715 T5714 compound Figure,7C
R3495 T5716 T5714 punct ),7C
R3496 T5717 T5654 punct .,was
R3497 T5719 T5720 det A,deficiency
R3498 T5720 T5722 nsubj deficiency,is
R3499 T5721 T5720 compound TGC,deficiency
R3500 T5723 T5722 prep in,is
R3501 T5724 T5725 det the,absence
R3502 T5725 T5723 pobj absence,in
R3503 T5726 T5725 prep of,absence
R3504 T5727 T5726 pobj Atrx,of
R3505 T5728 T5722 acomp consistent,is
R3506 T5729 T5728 prep with,consistent
R3507 T5730 T5731 det the,observation
R3508 T5731 T5729 pobj observation,with
R3509 T5732 T5733 mark that,is
R3510 T5733 T5731 acl is,observation
R3511 T5734 T5733 nsubj Atrx,is
R3512 T5735 T5736 advmod highly,expressed
R3513 T5736 T5733 acomp expressed,is
R3514 T5737 T5733 prep in,is
R3515 T5738 T5739 amod giant,cells
R3516 T5739 T5737 pobj cells,in
R3517 T5740 T5739 acl surrounding,cells
R3518 T5741 T5742 amod wild,type
R3519 T5742 T5744 nmod type,embryos
R3520 T5743 T5742 punct -,type
R3521 T5744 T5740 dobj embryos,surrounding
R3522 T5745 T5746 nummod 7.5,dpc
R3523 T5746 T5744 compound dpc,embryos
R3524 T5747 T5748 punct (,5E
R3525 T5748 T5722 parataxis 5E,is
R3526 T5749 T5748 compound Figure,5E
R3527 T5750 T5748 punct ),5E
R3528 T5751 T5722 punct .,is
R3529 T5753 T5754 aux To,investigate
R3530 T5754 T5755 advcl investigate,cultured
R3531 T5756 T5757 mark whether,restricted
R3532 T5757 T5754 ccomp restricted,investigate
R3533 T5758 T5759 det the,defect
R3534 T5759 T5757 nsubjpass defect,restricted
R3535 T5760 T5759 compound trophoblast,defect
R3536 T5761 T5757 auxpass was,restricted
R3537 T5762 T5757 prep to,restricted
R3538 T5763 T5764 det the,production
R3539 T5764 T5762 pobj production,to
R3540 T5765 T5764 prep of,production
R3541 T5766 T5767 amod secondary,TGCs
R3542 T5767 T5765 pobj TGCs,of
R3543 T5768 T5767 punct (,TGCs
R3544 T5769 T5767 acl produced,TGCs
R3545 T5770 T5769 agent by,produced
R3546 T5771 T5772 compound diploid,precursors
R3547 T5772 T5770 pobj precursors,by
R3548 T5773 T5769 prep in,produced
R3549 T5774 T5775 det the,cone
R3550 T5775 T5773 pobj cone,in
R3551 T5776 T5775 amod ectoplacental,cone
R3552 T5777 T5769 cc and,produced
R3553 T5778 T5769 conj derived,produced
R3554 T5779 T5778 advmod originally,derived
R3555 T5780 T5778 prep from,derived
R3556 T5781 T5782 det the,trophectoderm
R3557 T5782 T5780 pobj trophectoderm,from
R3558 T5783 T5782 amod polar,trophectoderm
R3559 T5784 T5782 acl overlying,trophectoderm
R3560 T5785 T5786 det the,mass
R3561 T5786 T5784 dobj mass,overlying
R3562 T5787 T5786 amod inner,mass
R3563 T5788 T5786 compound cell,mass
R3564 T5789 T5786 prep of,mass
R3565 T5790 T5791 det the,blastocyst
R3566 T5791 T5789 pobj blastocyst,of
R3567 T5792 T5757 punct ),restricted
R3568 T5793 T5757 cc or,restricted
R3569 T5794 T5795 advmod also,affected
R3570 T5795 T5757 conj affected,restricted
R3571 T5796 T5797 det the,production
R3572 T5797 T5795 dobj production,affected
R3573 T5798 T5797 prep of,production
R3574 T5799 T5800 amod primary,TGCs
R3575 T5800 T5798 pobj TGCs,of
R3576 T5801 T5800 punct (,TGCs
R3577 T5802 T5800 acl resulting,TGCs
R3578 T5803 T5802 prep from,resulting
R3579 T5804 T5803 pobj differentiation,from
R3580 T5805 T5804 prep of,differentiation
R3581 T5806 T5807 det the,trophectoderm
R3582 T5807 T5805 pobj trophectoderm,of
R3583 T5808 T5807 amod mural,trophectoderm
R3584 T5809 T5807 prep of,trophectoderm
R3585 T5810 T5811 det the,blastocyst
R3586 T5811 T5809 pobj blastocyst,of
R3587 T5812 T5755 punct ),cultured
R3588 T5813 T5755 punct ", ",cultured
R3589 T5814 T5755 nsubjpass blastocysts,cultured
R3590 T5815 T5816 punct (,dpc
R3591 T5816 T5814 parataxis dpc,blastocysts
R3592 T5817 T5816 nummod 3.5,dpc
R3593 T5818 T5816 punct ),dpc
R3594 T5819 T5814 prep from,blastocysts
R3595 T5820 T5819 pobj crosses,from
R3596 T5821 T5820 prep between,crosses
R3597 T5822 T5823 compound Atrx WT,flox
R3598 T5823 T5825 compound flox,females
R3599 T5824 T5823 punct /,flox
R3600 T5825 T5821 pobj females,between
R3601 T5826 T5825 cc and,females
R3602 T5827 T5828 compound GATA1,Cre
R3603 T5828 T5830 npadvmod Cre,homozygous
R3604 T5829 T5828 punct -,Cre
R3605 T5830 T5831 amod homozygous,males
R3606 T5831 T5825 conj males,females
R3607 T5832 T5831 amod transgenic,males
R3608 T5833 T5825 punct (,females
R3609 T5834 T5835 compound Atrx WT,Y
R3610 T5835 T5825 appos Y,females
R3611 T5836 T5835 punct /,Y
R3612 T5837 T5835 punct ;,Y
R3613 T5838 T5839 compound GATA1,Cre
R3614 T5839 T5835 appos Cre,Y
R3615 T5840 T5839 punct -,Cre
R3616 T5841 T5839 punct +,Cre
R3617 T5842 T5839 punct /,Cre
R3618 T5843 T5839 punct +,Cre
R3619 T5844 T5755 punct ),cultured
R3620 T5845 T5755 auxpass were,cultured
R3621 T5846 T5847 advmod in,vitro
R3622 T5847 T5755 advmod vitro,cultured
R3623 T5848 T5755 prep for,cultured
R3624 T5849 T5850 nummod 5,d
R3625 T5850 T5848 pobj d,for
R3626 T5851 T5852 aux to,monitor
R3627 T5852 T5755 advcl monitor,cultured
R3628 T5853 T5852 dobj outgrowth,monitor
R3629 T5854 T5853 prep of,outgrowth
R3630 T5855 T5856 det the,trophoblast
R3631 T5856 T5854 pobj trophoblast,of
R3632 T5857 T5856 amod primary,trophoblast
R3633 T5858 T5755 punct .,cultured
R3634 T5860 T5861 prep After,scored
R3635 T5862 T5863 nummod 5,d
R3636 T5863 T5860 pobj d,After
R3637 T5864 T5861 punct ", ",scored
R3638 T5865 T5866 amod individual,cultures
R3639 T5866 T5861 nsubjpass cultures,scored
R3640 T5867 T5866 compound blastocyst,cultures
R3641 T5868 T5861 auxpass were,scored
R3642 T5869 T5861 prep for,scored
R3643 T5870 T5871 det the,extent
R3644 T5871 T5869 pobj extent,for
R3645 T5872 T5871 prep of,extent
R3646 T5873 T5874 amod primary,outgrowth
R3647 T5874 T5872 pobj outgrowth,of
R3648 T5875 T5874 compound trophoblast,outgrowth
R3649 T5876 T5861 punct ", ",scored
R3650 T5877 T5861 cc and,scored
R3651 T5878 T5879 det the,genotype
R3652 T5879 T5881 nsubjpass genotype,determined
R3653 T5880 T5879 compound Atrx,genotype
R3654 T5881 T5861 conj determined,scored
R3655 T5882 T5879 cc and,genotype
R3656 T5883 T5879 conj sex,genotype
R3657 T5884 T5883 prep of,sex
R3658 T5885 T5886 det the,blastocyst
R3659 T5886 T5884 pobj blastocyst,of
R3660 T5887 T5881 auxpass were,determined
R3661 T5888 T5881 prep by,determined
R3662 T5889 T5888 pobj PCR,by
R3663 T5890 T5861 punct .,scored
R3664 T5892 T5893 amod Most,blastocysts
R3665 T5893 T5894 nsubj blastocysts,hatched
R3666 T5895 T5894 prep from,hatched
R3667 T5896 T5897 det the,pellucida
R3668 T5897 T5895 pobj pellucida,from
R3669 T5898 T5897 compound zona,pellucida
R3670 T5899 T5894 prep within,hatched
R3671 T5900 T5901 nummod 24,h
R3672 T5901 T5899 pobj h,within
R3673 T5902 T5894 punct ", ",hatched
R3674 T5903 T5894 cc and,hatched
R3675 T5904 T5905 compound trophoblast,cells
R3676 T5905 T5906 nsubjpass cells,detected
R3677 T5906 T5894 conj detected,hatched
R3678 T5907 T5905 acl spreading,cells
R3679 T5908 T5907 prt out,spreading
R3680 T5909 T5907 prep from,spreading
R3681 T5910 T5911 det the,mass
R3682 T5911 T5909 pobj mass,from
R3683 T5912 T5911 amod inner,mass
R3684 T5913 T5911 compound cell,mass
R3685 T5914 T5906 aux could,detected
R3686 T5915 T5906 advmod usually,detected
R3687 T5916 T5906 auxpass be,detected
R3688 T5917 T5906 prep within,detected
R3689 T5918 T5919 nummod 48,h
R3690 T5919 T5917 pobj h,within
R3691 T5920 T5919 prep of,h
R3692 T5921 T5920 pobj culture,of
R3693 T5922 T5906 punct .,detected
R3694 T5924 T5925 det No,difference
R3695 T5925 T5926 nsubjpass difference,observed
R3696 T5927 T5926 auxpass was,observed
R3697 T5928 T5926 prep in,observed
R3698 T5929 T5930 det the,rate
R3699 T5930 T5928 pobj rate,in
R3700 T5931 T5930 cc or,rate
R3701 T5932 T5930 conj extent,rate
R3702 T5933 T5930 prep of,rate
R3703 T5934 T5935 compound trophoblast,outgrowth
R3704 T5935 T5933 pobj outgrowth,of
R3705 T5936 T5926 prep over,observed
R3706 T5937 T5938 nummod 5,d
R3707 T5938 T5936 pobj d,over
R3708 T5939 T5938 prep of,d
R3709 T5940 T5939 pobj culture,of
R3710 T5941 T5926 prep between,observed
R3711 T5942 T5943 amod Atrxnull,blastocysts
R3712 T5943 T5941 pobj blastocysts,between
R3713 T5944 T5945 punct (,Y
R3714 T5945 T5943 parataxis Y,blastocysts
R3715 T5946 T5945 amod Atrx null,Y
R3716 T5947 T5945 punct /,Y
R3717 T5948 T5945 punct ", ",Y
R3718 T5949 T5950 nsubj n,6
R3719 T5950 T5945 ccomp 6,Y
R3720 T5951 T5950 punct =,6
R3721 T5952 T5945 punct ),Y
R3722 T5953 T5943 cc and,blastocysts
R3723 T5954 T5943 conj blastocysts,blastocysts
R3724 T5955 T5954 acl bearing,blastocysts
R3725 T5956 T5957 det an,allele
R3726 T5957 T5955 dobj allele,bearing
R3727 T5958 T5957 compound Atrx WT,allele
R3728 T5959 T5960 punct (,6
R3729 T5960 T5954 parataxis 6,blastocysts
R3730 T5961 T5962 compound Atrx WT,WT
R3731 T5962 T5964 dep WT,6
R3732 T5963 T5962 punct /,WT
R3733 T5964 T5960 dep 6,6
R3734 T5965 T5964 punct ", ",6
R3735 T5966 T5964 nsubj n,6
R3736 T5967 T5964 punct =,6
R3737 T5968 T5960 punct ;,6
R3738 T5969 T5970 compound Atrx WT,null
R3739 T5970 T5972 dep null,6
R3740 T5971 T5970 punct /,null
R3741 T5972 T5960 dep 6,6
R3742 T5973 T5972 punct ", ",6
R3743 T5974 T5972 nsubj n,6
R3744 T5975 T5972 punct =,6
R3745 T5976 T5960 punct ;,6
R3746 T5977 T5978 compound Atrx WT,Y
R3747 T5978 T5960 dep Y,6
R3748 T5979 T5978 punct /,Y
R3749 T5980 T5960 punct ", ",6
R3750 T5981 T5960 nsubj n,6
R3751 T5982 T5960 punct =,6
R3752 T5983 T5960 punct ),6
R3753 T5984 T5985 punct (,shown
R3754 T5985 T5954 parataxis shown,blastocysts
R3755 T5986 T5985 nsubj examples,shown
R3756 T5987 T5985 prep in,shown
R3757 T5988 T5989 compound Figure,7D
R3758 T5989 T5987 pobj 7D,in
R3759 T5990 T5985 punct ),shown
R3760 T5991 T5926 punct ", ",observed
R3761 T5992 T5926 advcl suggesting,observed
R3762 T5993 T5994 mark that,involves
R3763 T5994 T5992 ccomp involves,suggesting
R3764 T5995 T5996 det the,defect
R3765 T5996 T5994 nsubj defect,involves
R3766 T5997 T5994 advmod specifically,involves
R3767 T5998 T5999 det the,compartment
R3768 T5999 T5994 dobj compartment,involves
R3769 T6000 T5999 amod secondary,compartment
R3770 T6001 T5999 amod giant,compartment
R3771 T6002 T5999 compound cell,compartment
R3772 T6003 T5926 punct .,observed
R3773 T6005 T6006 nsubj This,is
R3774 T6007 T6006 acomp consistent,is
R3775 T6008 T6007 prep with,consistent
R3776 T6009 T6010 det the,observation
R3777 T6010 T6008 pobj observation,with
R3778 T6011 T6012 mark that,implant
R3779 T6012 T6010 acl implant,observation
R3780 T6013 T6014 amod Atrxnull,conceptuses
R3781 T6014 T6012 nsubj conceptuses,implant
R3782 T6015 T6012 advmod successfully,implant
R3783 T6016 T6012 cc and,implant
R3784 T6017 T6012 conj survive,implant
R3785 T6018 T6017 prep to,survive
R3786 T6019 T6018 pobj gastrulation,to
R3787 T6020 T6006 punct .,is
R3788 T6022 T6023 advcl Taken,suggest
R3789 T6024 T6022 advmod together,Taken
R3790 T6025 T6023 punct ", ",suggest
R3791 T6026 T6027 det these,data
R3792 T6027 T6023 nsubj data,suggest
R3793 T6028 T6029 mark that,results
R3794 T6029 T6023 ccomp results,suggest
R3795 T6030 T6029 nsubj loss,results
R3796 T6031 T6030 prep of,loss
R3797 T6032 T6031 pobj Atrx,of
R3798 T6033 T6029 prep in,results
R3799 T6034 T6035 det a,defect
R3800 T6035 T6033 pobj defect,in
R3801 T6036 T6035 prep in,defect
R3802 T6037 T6036 pobj formation,in
R3803 T6038 T6037 prep of,formation
R3804 T6039 T6040 det the,trophoblast
R3805 T6040 T6038 pobj trophoblast,of
R3806 T6041 T6040 amod secondary,trophoblast
R3807 T6042 T6043 dep that,is
R3808 T6043 T6035 relcl is,defect
R3809 T6044 T6043 acomp apparent,is
R3810 T6045 T6043 prep from,is
R3811 T6046 T6047 nummod 7.5,dpc
R3812 T6047 T6045 pobj dpc,from
R3813 T6048 T6023 punct .,suggest
R3814 T6050 T6051 prep Despite,exhibit
R3815 T6052 T6050 pcomp initiating,Despite
R3816 T6053 T6054 amod normal,organisation
R3817 T6054 T6052 dobj organisation,initiating
R3818 T6055 T6052 prep in,initiating
R3819 T6056 T6057 det the,embryo
R3820 T6057 T6055 pobj embryo,in
R3821 T6058 T6057 amod proper,embryo
R3822 T6059 T6051 punct ", ",exhibit
R3823 T6060 T6061 amod Atrxnull,conceptuses
R3824 T6061 T6051 nsubj conceptuses,exhibit
R3825 T6062 T6063 det a,defect
R3826 T6063 T6051 dobj defect,exhibit
R3827 T6064 T6063 amod proliferative,defect
R3828 T6065 T6051 prep by,exhibit
R3829 T6066 T6067 nummod 7.5,dpc
R3830 T6067 T6065 pobj dpc,by
R3831 T6068 T6051 cc and,exhibit
R3832 T6069 T6051 conj die,exhibit
R3833 T6070 T6069 prep by,die
R3834 T6071 T6072 quantmod around,9.5
R3835 T6072 T6073 nummod 9.5,dpc
R3836 T6073 T6070 pobj dpc,by
R3837 T6074 T6051 punct ", ",exhibit
R3838 T6075 T6076 advmod probably,due
R3839 T6076 T6051 prep due,exhibit
R3840 T6077 T6076 pcomp to,due
R3841 T6078 T6079 det a,deficit
R3842 T6079 T6076 pobj deficit,due
R3843 T6080 T6079 amod nutritional,deficit
R3844 T6081 T6079 acl resulting,deficit
R3845 T6082 T6081 prep from,resulting
R3846 T6083 T6082 pobj failure,from
R3847 T6084 T6085 aux to,develop
R3848 T6085 T6083 acl develop,failure
R3849 T6086 T6087 det a,trophoblast
R3850 T6087 T6085 dobj trophoblast,develop
R3851 T6088 T6087 amod normal,trophoblast
R3852 T6089 T6051 punct .,exhibit