PMC:1435744 / 7013-31435 JSON TXT 4 Projects

Annnotations TAB TSV DIC JSON TextAE

Id	Subject	Object	Predicate	Lexical cue
T5546	9-16	NN	denotes	Cloning
T5547	17-19	IN	denotes	of
T5548	20-25	NN	denotes	mouse
T5550	26-28	NN	denotes	mr
T5551	28-29	HYPH	denotes	-
T5549	29-30	NN	denotes	s
T5552	30-338	sentence	denotes	In order to identify novel mouse genes preferentially expressed in the developing retina, we screened the National Institute for Biotechnology Information (NCBI) database, UniGene, using Digital Differential Display (DDD) and found EST fragments which are frequently present in mouse retinal cDNA libraries.
T5553	31-33	IN	denotes	In
T5555	34-39	NN	denotes	order
T5556	40-42	TO	denotes	to
T5557	43-51	VB	denotes	identify
T5558	52-57	JJ	denotes	novel
T5560	58-63	NN	denotes	mouse
T5559	64-69	NNS	denotes	genes
T5561	70-84	RB	denotes	preferentially
T5562	85-94	VBN	denotes	expressed
T5563	95-97	IN	denotes	in
T5564	98-101	DT	denotes	the
T5566	102-112	VBG	denotes	developing
T5565	113-119	NN	denotes	retina
T5567	119-121	,	denotes	,
T5568	121-123	PRP	denotes	we
T5554	124-132	VBD	denotes	screened
T5569	133-136	DT	denotes	the
T5571	137-145	NNP	denotes	National
T5572	146-155	NNP	denotes	Institute
T5573	156-159	IN	denotes	for
T5574	160-173	NNP	denotes	Biotechnology
T5575	174-185	NNP	denotes	Information
T5576	186-187	-LRB-	denotes	(
T5577	187-191	NNP	denotes	NCBI
T5578	191-192	-RRB-	denotes	)
T5570	193-201	NN	denotes	database
T5579	201-203	,	denotes	,
T5580	203-210	NNP	denotes	UniGene
T5581	210-212	,	denotes	,
T5582	212-217	VBG	denotes	using
T5583	218-225	NNP	denotes	Digital
T5585	226-238	NNP	denotes	Differential
T5584	239-246	NNP	denotes	Display
T5586	247-248	-LRB-	denotes	(
T5587	248-251	NNP	denotes	DDD
T5588	251-252	-RRB-	denotes	)
T5589	253-256	CC	denotes	and
T5590	257-262	VBD	denotes	found
T5591	263-266	NN	denotes	EST
T5592	267-276	NNS	denotes	fragments
T5593	277-282	WDT	denotes	which
T5594	283-286	VBP	denotes	are
T5595	287-297	RB	denotes	frequently
T5596	298-305	JJ	denotes	present
T5597	306-308	IN	denotes	in
T5598	309-314	NN	denotes	mouse
T5600	315-322	JJ	denotes	retinal
T5601	323-327	NN	denotes	cDNA
T5599	328-337	NNS	denotes	libraries
T5602	337-338	.	denotes	.
T5603	338-460	sentence	denotes	We found that one clone in these cDNAs encodes a protein containing a SAM domain related to that of polyhomeotic protein.
T5604	339-341	PRP	denotes	We
T5605	342-347	VBD	denotes	found
T5606	348-352	IN	denotes	that
T5608	353-356	CD	denotes	one
T5609	357-362	NN	denotes	clone
T5610	363-365	IN	denotes	in
T5611	366-371	DT	denotes	these
T5612	372-377	NNS	denotes	cDNAs
T5607	378-385	VBZ	denotes	encodes
T5613	386-387	DT	denotes	a
T5614	388-395	NN	denotes	protein
T5615	396-406	VBG	denotes	containing
T5616	407-408	DT	denotes	a
T5618	409-412	NN	denotes	SAM
T5617	413-419	NN	denotes	domain
T5619	420-427	VBN	denotes	related
T5620	428-430	IN	denotes	to
T5621	431-435	DT	denotes	that
T5622	436-438	IN	denotes	of
T5623	439-451	JJ	denotes	polyhomeotic
T5624	452-459	NN	denotes	protein
T5625	459-460	.	denotes	.
T5626	460-599	sentence	denotes	A PCR fragment corresponding to this mouse clone was used to screen a mouse P0-P3 retinal cDNA library to obtain a full-length cDNA clone.
T5627	461-462	DT	denotes	A
T5629	463-466	NN	denotes	PCR
T5628	467-475	NN	denotes	fragment
T5631	476-489	VBG	denotes	corresponding
T5632	490-492	IN	denotes	to
T5633	493-497	DT	denotes	this
T5635	498-503	NN	denotes	mouse
T5634	504-509	NN	denotes	clone
T5636	510-513	VBD	denotes	was
T5630	514-518	VBN	denotes	used
T5637	519-521	TO	denotes	to
T5638	522-528	VB	denotes	screen
T5639	529-530	DT	denotes	a
T5641	531-536	NN	denotes	mouse
T5642	537-539	NN	denotes	P0
T5644	539-540	SYM	denotes	-
T5643	540-542	NN	denotes	P3
T5645	543-550	JJ	denotes	retinal
T5646	551-555	NN	denotes	cDNA
T5640	556-563	NN	denotes	library
T5647	564-566	TO	denotes	to
T5648	567-573	VB	denotes	obtain
T5649	574-575	DT	denotes	a
T5651	576-580	JJ	denotes	full
T5653	580-581	HYPH	denotes	-
T5652	581-587	NN	denotes	length
T5654	588-592	NN	denotes	cDNA
T5650	593-598	NN	denotes	clone
T5655	598-599	.	denotes	.
T5656	599-698	sentence	denotes	Sequence analysis showed that this cDNA was a novel gene encoding a SAM domain-containing protein.
T5657	600-608	NN	denotes	Sequence
T5658	609-617	NN	denotes	analysis
T5659	618-624	VBD	denotes	showed
T5660	625-629	IN	denotes	that
T5662	630-634	DT	denotes	this
T5663	635-639	NN	denotes	cDNA
T5661	640-643	VBD	denotes	was
T5664	644-645	DT	denotes	a
T5666	646-651	JJ	denotes	novel
T5665	652-656	NN	denotes	gene
T5667	657-665	VBG	denotes	encoding
T5668	666-667	DT	denotes	a
T5670	668-671	NN	denotes	SAM
T5671	672-678	NN	denotes	domain
T5673	678-679	HYPH	denotes	-
T5672	679-689	VBG	denotes	containing
T5669	690-697	NN	denotes	protein
T5674	697-698	.	denotes	.
T5675	698-770	sentence	denotes	We referred to this protein as mr-s (major retinal SAM domain protein).
T5676	699-701	PRP	denotes	We
T5677	702-710	VBD	denotes	referred
T5678	711-713	IN	denotes	to
T5679	714-718	DT	denotes	this
T5680	719-726	NN	denotes	protein
T5681	727-729	IN	denotes	as
T5682	730-732	NN	denotes	mr
T5684	732-733	HYPH	denotes	-
T5683	733-734	NN	denotes	s
T5685	735-736	-LRB-	denotes	(
T5686	736-741	JJ	denotes	major
T5688	742-749	JJ	denotes	retinal
T5689	750-753	NN	denotes	SAM
T5690	754-760	NN	denotes	domain
T5687	761-768	NN	denotes	protein
T5691	768-769	-RRB-	denotes	)
T5692	769-770	.	denotes	.
T5693	770-883	sentence	denotes	As shown in Fig. 1A, a translation initiation codon is present in the same open reading frame as the SAM domain.
T5694	771-773	IN	denotes	As
T5695	774-779	VBN	denotes	shown
T5697	780-782	IN	denotes	in
T5698	783-787	NN	denotes	Fig.
T5699	788-790	NN	denotes	1A
T5700	790-792	,	denotes	,
T5701	792-793	DT	denotes	a
T5703	794-805	NN	denotes	translation
T5704	806-816	NN	denotes	initiation
T5702	817-822	NN	denotes	codon
T5696	823-825	VBZ	denotes	is
T5705	826-833	JJ	denotes	present
T5706	834-836	IN	denotes	in
T5707	837-840	DT	denotes	the
T5709	841-845	JJ	denotes	same
T5710	846-850	JJ	denotes	open
T5711	851-858	NN	denotes	reading
T5708	859-864	NN	denotes	frame
T5712	865-867	IN	denotes	as
T5713	868-871	DT	denotes	the
T5715	872-875	NN	denotes	SAM
T5714	876-882	NN	denotes	domain
T5716	882-883	.	denotes	.
T5717	883-1040	sentence	denotes	This initiation site shows similarity to the consensus sequence proposed by Kozak [32] including the presence of the highly conserved purine at position -3.
T5718	884-888	DT	denotes	This
T5720	889-899	NN	denotes	initiation
T5719	900-904	NN	denotes	site
T5721	905-910	VBZ	denotes	shows
T5722	911-921	NN	denotes	similarity
T5723	922-924	IN	denotes	to
T5724	925-928	DT	denotes	the
T5726	929-938	NN	denotes	consensus
T5725	939-947	NN	denotes	sequence
T5727	948-956	VBN	denotes	proposed
T5728	957-959	IN	denotes	by
T5729	960-965	NN	denotes	Kozak
T5730	966-967	-LRB-	denotes	[
T5731	967-969	CD	denotes	32
T5732	969-970	-RRB-	denotes	]
T5733	971-980	VBG	denotes	including
T5734	981-984	DT	denotes	the
T5735	985-993	NN	denotes	presence
T5736	994-996	IN	denotes	of
T5737	997-1000	DT	denotes	the
T5739	1001-1007	RB	denotes	highly
T5740	1008-1017	VBN	denotes	conserved
T5738	1018-1024	NN	denotes	purine
T5741	1025-1027	IN	denotes	at
T5742	1028-1036	NN	denotes	position
T5743	1037-1038	SYM	denotes	-
T5744	1038-1039	CD	denotes	3
T5745	1039-1040	.	denotes	.
T5746	1040-1115	sentence	denotes	The stop codon of the predicted mr-s protein is also indicated in Fig. 1A.
T5747	1041-1044	DT	denotes	The
T5749	1045-1049	NN	denotes	stop
T5748	1050-1055	NN	denotes	codon
T5751	1056-1058	IN	denotes	of
T5752	1059-1062	DT	denotes	the
T5754	1063-1072	VBN	denotes	predicted
T5755	1073-1075	NN	denotes	mr
T5757	1075-1076	HYPH	denotes	-
T5756	1076-1077	NN	denotes	s
T5753	1078-1085	NN	denotes	protein
T5758	1086-1088	VBZ	denotes	is
T5759	1089-1093	RB	denotes	also
T5750	1094-1103	VBN	denotes	indicated
T5760	1104-1106	IN	denotes	in
T5761	1107-1111	NN	denotes	Fig.
T5762	1112-1114	NN	denotes	1A
T5763	1114-1115	.	denotes	.
T5764	1115-1282	sentence	denotes	The amino acid sequence of the SAM domain of mr-s protein (Fig. 1A, boxed sequence) exhibits homology with SAM domains of EphB2, EphA4, MPH1, TEL and Smaug (Fig. 1B).
T5765	1116-1119	DT	denotes	The
T5767	1120-1125	NN	denotes	amino
T5768	1126-1130	NN	denotes	acid
T5766	1131-1139	NN	denotes	sequence
T5770	1140-1142	IN	denotes	of
T5771	1143-1146	DT	denotes	the
T5773	1147-1150	NN	denotes	SAM
T5772	1151-1157	NN	denotes	domain
T5774	1158-1160	IN	denotes	of
T5775	1161-1163	NN	denotes	mr
T5777	1163-1164	HYPH	denotes	-
T5776	1164-1165	NN	denotes	s
T5778	1166-1173	NN	denotes	protein
T5779	1174-1175	-LRB-	denotes	(
T5781	1175-1179	NN	denotes	Fig.
T5780	1180-1182	NN	denotes	1A
T5782	1182-1184	,	denotes	,
T5783	1184-1189	VBN	denotes	boxed
T5784	1190-1198	NN	denotes	sequence
T5785	1198-1199	-RRB-	denotes	)
T5769	1200-1208	VBZ	denotes	exhibits
T5786	1209-1217	NN	denotes	homology
T5787	1218-1222	IN	denotes	with
T5788	1223-1226	NN	denotes	SAM
T5789	1227-1234	NNS	denotes	domains
T5790	1235-1237	IN	denotes	of
T5791	1238-1243	NN	denotes	EphB2
T5792	1243-1245	,	denotes	,
T5793	1245-1250	NN	denotes	EphA4
T5794	1250-1252	,	denotes	,
T5795	1252-1256	NN	denotes	MPH1
T5796	1256-1258	,	denotes	,
T5797	1258-1261	NN	denotes	TEL
T5798	1262-1265	CC	denotes	and
T5799	1266-1271	NN	denotes	Smaug
T5800	1272-1273	-LRB-	denotes	(
T5802	1273-1277	NN	denotes	Fig.
T5801	1278-1280	NN	denotes	1B
T5803	1280-1281	-RRB-	denotes	)
T5804	1281-1282	.	denotes	.
T5805	1282-1411	sentence	denotes	By phylogenetic analysis, the SAM domain of mr-s is most closely related to that of Mph1/Rae28, a mouse homolog of ph (Fig. 1C).
T5806	1283-1285	IN	denotes	By
T5808	1286-1298	JJ	denotes	phylogenetic
T5809	1299-1307	NN	denotes	analysis
T5810	1307-1309	,	denotes	,
T5811	1309-1312	DT	denotes	the
T5813	1313-1316	NN	denotes	SAM
T5812	1317-1323	NN	denotes	domain
T5814	1324-1326	IN	denotes	of
T5815	1327-1329	NN	denotes	mr
T5817	1329-1330	HYPH	denotes	-
T5816	1330-1331	NN	denotes	s
T5818	1332-1334	VBZ	denotes	is
T5819	1335-1339	RBS	denotes	most
T5820	1340-1347	RB	denotes	closely
T5807	1348-1355	VBN	denotes	related
T5821	1356-1358	IN	denotes	to
T5822	1359-1363	DT	denotes	that
T5823	1364-1366	IN	denotes	of
T5824	1367-1371	NN	denotes	Mph1
T5826	1371-1372	HYPH	denotes	/
T5825	1372-1377	NN	denotes	Rae28
T5827	1377-1379	,	denotes	,
T5828	1379-1380	DT	denotes	a
T5830	1381-1386	NN	denotes	mouse
T5829	1387-1394	NN	denotes	homolog
T5831	1395-1397	IN	denotes	of
T5832	1398-1400	NN	denotes	ph
T5833	1401-1402	-LRB-	denotes	(
T5835	1402-1406	NN	denotes	Fig.
T5834	1407-1409	NN	denotes	1C
T5836	1409-1410	-RRB-	denotes	)
T5837	1410-1411	.	denotes	.
T5838	1411-1565	sentence	denotes	Mouse mr-s protein is conserved in rat, human, chick and zebrafish, which display 91%, 70%, 36% and 26% identity with mouse mr-s, respectively (Fig. 1D).
T5839	1412-1417	NN	denotes	Mouse
T5841	1418-1420	NN	denotes	mr
T5843	1420-1421	HYPH	denotes	-
T5842	1421-1422	NN	denotes	s
T5840	1423-1430	NN	denotes	protein
T5845	1431-1433	VBZ	denotes	is
T5844	1434-1443	VBN	denotes	conserved
T5846	1444-1446	IN	denotes	in
T5847	1447-1450	NN	denotes	rat
T5848	1450-1452	,	denotes	,
T5849	1452-1457	JJ	denotes	human
T5850	1457-1459	,	denotes	,
T5851	1459-1464	NN	denotes	chick
T5852	1465-1468	CC	denotes	and
T5853	1469-1478	NN	denotes	zebrafish
T5854	1478-1480	,	denotes	,
T5855	1480-1485	WDT	denotes	which
T5856	1486-1493	VBP	denotes	display
T5857	1494-1496	CD	denotes	91
T5858	1496-1497	NN	denotes	%
T5860	1497-1499	,	denotes	,
T5861	1499-1501	CD	denotes	70
T5862	1501-1502	NN	denotes	%
T5863	1502-1504	,	denotes	,
T5864	1504-1506	CD	denotes	36
T5865	1506-1507	NN	denotes	%
T5866	1508-1511	CC	denotes	and
T5867	1512-1514	CD	denotes	26
T5868	1514-1515	NN	denotes	%
T5859	1516-1524	NN	denotes	identity
T5869	1525-1529	IN	denotes	with
T5870	1530-1535	NN	denotes	mouse
T5872	1536-1538	NN	denotes	mr
T5873	1538-1539	HYPH	denotes	-
T5871	1539-1540	NN	denotes	s
T5874	1540-1542	,	denotes	,
T5875	1542-1554	RB	denotes	respectively
T5876	1555-1556	-LRB-	denotes	(
T5878	1556-1560	NN	denotes	Fig.
T5877	1561-1563	NN	denotes	1D
T5879	1563-1564	-RRB-	denotes	)
T5880	1564-1565	.	denotes	.
T5881	1565-1736	sentence	denotes	The SAM domains of rat, human, chick and zebrafish mr-s protein are highly conserved and display 96%, 90%, 76% and 72% identity with the SAM domain of mouse mr-s protein.
T5882	1566-1569	DT	denotes	The
T5884	1570-1573	NN	denotes	SAM
T5883	1574-1581	NNS	denotes	domains
T5886	1582-1584	IN	denotes	of
T5887	1585-1588	NN	denotes	rat
T5889	1588-1590	,	denotes	,
T5890	1590-1595	JJ	denotes	human
T5891	1595-1597	,	denotes	,
T5892	1597-1602	NN	denotes	chick
T5893	1603-1606	CC	denotes	and
T5894	1607-1616	NN	denotes	zebrafish
T5895	1617-1619	NN	denotes	mr
T5897	1619-1620	HYPH	denotes	-
T5896	1620-1621	NN	denotes	s
T5888	1622-1629	NN	denotes	protein
T5898	1630-1633	VBP	denotes	are
T5899	1634-1640	RB	denotes	highly
T5885	1641-1650	VBN	denotes	conserved
T5900	1651-1654	CC	denotes	and
T5901	1655-1662	VBP	denotes	display
T5902	1663-1665	CD	denotes	96
T5903	1665-1666	NN	denotes	%
T5905	1666-1668	,	denotes	,
T5906	1668-1670	CD	denotes	90
T5907	1670-1671	NN	denotes	%
T5908	1671-1673	,	denotes	,
T5909	1673-1675	CD	denotes	76
T5910	1675-1676	NN	denotes	%
T5911	1677-1680	CC	denotes	and
T5912	1681-1683	CD	denotes	72
T5913	1683-1684	NN	denotes	%
T5904	1685-1693	NN	denotes	identity
T5914	1694-1698	IN	denotes	with
T5915	1699-1702	DT	denotes	the
T5917	1703-1706	NN	denotes	SAM
T5916	1707-1713	NN	denotes	domain
T5918	1714-1716	IN	denotes	of
T5919	1717-1722	NN	denotes	mouse
T5921	1723-1725	NN	denotes	mr
T5923	1725-1726	HYPH	denotes	-
T5922	1726-1727	NN	denotes	s
T5920	1728-1735	NN	denotes	protein
T5924	1735-1736	.	denotes	.
T5925	1736-1884	sentence	denotes	The chromosomal localizations of mouse and human mr-s genes were determined by searching the mouse and human genome databases (NCBI), respectively.
T5926	1737-1740	DT	denotes	The
T5928	1741-1752	JJ	denotes	chromosomal
T5927	1753-1766	NNS	denotes	localizations
T5930	1767-1769	IN	denotes	of
T5931	1770-1775	NN	denotes	mouse
T5933	1776-1779	CC	denotes	and
T5934	1780-1785	JJ	denotes	human
T5935	1786-1788	NN	denotes	mr
T5937	1788-1789	HYPH	denotes	-
T5936	1789-1790	NN	denotes	s
T5932	1791-1796	NNS	denotes	genes
T5938	1797-1801	VBD	denotes	were
T5929	1802-1812	VBN	denotes	determined
T5939	1813-1815	IN	denotes	by
T5940	1816-1825	VBG	denotes	searching
T5941	1826-1829	DT	denotes	the
T5943	1830-1835	NN	denotes	mouse
T5944	1836-1839	CC	denotes	and
T5945	1840-1845	JJ	denotes	human
T5946	1846-1852	NN	denotes	genome
T5942	1853-1862	NNS	denotes	databases
T5947	1863-1864	-LRB-	denotes	(
T5948	1864-1868	NN	denotes	NCBI
T5949	1868-1869	-RRB-	denotes	)
T5950	1869-1871	,	denotes	,
T5951	1871-1883	RB	denotes	respectively
T5952	1883-1884	.	denotes	.
T5953	1884-1972	sentence	denotes	Mouse mr-s is mapped to chromosome 4E2, and human MR-S is mapped to chromosome 1p36.33.
T5954	1885-1890	NN	denotes	Mouse
T5956	1891-1893	NN	denotes	mr
T5957	1893-1894	HYPH	denotes	-
T5955	1894-1895	NN	denotes	s
T5959	1896-1898	VBZ	denotes	is
T5958	1899-1905	VBN	denotes	mapped
T5960	1906-1908	IN	denotes	to
T5961	1909-1919	NN	denotes	chromosome
T5962	1920-1923	NN	denotes	4E2
T5963	1923-1925	,	denotes	,
T5964	1925-1928	CC	denotes	and
T5965	1929-1934	JJ	denotes	human
T5967	1935-1937	NN	denotes	MR
T5968	1937-1938	HYPH	denotes	-
T5966	1938-1939	NN	denotes	S
T5970	1940-1942	VBZ	denotes	is
T5969	1943-1949	VBN	denotes	mapped
T5971	1950-1952	IN	denotes	to
T5972	1953-1963	NN	denotes	chromosome
T5973	1964-1971	NN	denotes	1p36.33
T5974	1971-1972	.	denotes	.
T5975	1972-2035	sentence	denotes	LCA is the most common cause of inherited childhood blindness.
T5976	1973-1976	NN	denotes	LCA
T5977	1977-1979	VBZ	denotes	is
T5978	1980-1983	DT	denotes	the
T5980	1984-1988	RBS	denotes	most
T5981	1989-1995	JJ	denotes	common
T5979	1996-2001	NN	denotes	cause
T5982	2002-2004	IN	denotes	of
T5983	2005-2014	VBN	denotes	inherited
T5985	2015-2024	NN	denotes	childhood
T5984	2025-2034	NN	denotes	blindness
T5986	2034-2035	.	denotes	.
T5987	2035-2136	sentence	denotes	Human MR-S maps in the vicinity region of the LCA9, recently identified as a new locus for LCA [33].
T5988	2036-2041	JJ	denotes	Human
T5990	2042-2044	NN	denotes	MR
T5991	2044-2045	HYPH	denotes	-
T5989	2045-2046	NN	denotes	S
T5992	2047-2051	VBZ	denotes	maps
T5993	2052-2054	IN	denotes	in
T5994	2055-2058	DT	denotes	the
T5996	2059-2067	NN	denotes	vicinity
T5995	2068-2074	NN	denotes	region
T5997	2075-2077	IN	denotes	of
T5998	2078-2081	DT	denotes	the
T5999	2082-2086	NN	denotes	LCA9
T6000	2086-2088	,	denotes	,
T6001	2088-2096	RB	denotes	recently
T6002	2097-2107	VBN	denotes	identified
T6003	2108-2110	IN	denotes	as
T6004	2111-2112	DT	denotes	a
T6006	2113-2116	JJ	denotes	new
T6005	2117-2122	NN	denotes	locus
T6007	2123-2126	IN	denotes	for
T6008	2127-2130	NN	denotes	LCA
T6009	2131-2132	-LRB-	denotes	[
T6010	2132-2134	CD	denotes	33
T6011	2134-2135	-RRB-	denotes	]
T6012	2135-2136	.	denotes	.
T28701	2147-2149	NN	denotes	mr
T28703	2149-2150	HYPH	denotes	-
T28702	2150-2151	NN	denotes	s
T28704	2152-2162	NN	denotes	nucleotide
T28706	2163-2166	CC	denotes	and
T28707	2167-2172	NN	denotes	amino
T28708	2173-2177	NN	denotes	acid
T28705	2178-2187	NNS	denotes	sequences
T28709	2187-2188	.	denotes	.
T28710	2188-2235	sentence	denotes	(A) mr-s nucleotide and amino acids sequences.
T28711	2189-2190	-LRB-	denotes	(
T28712	2190-2191	LS	denotes	A
T28714	2191-2192	-RRB-	denotes	)
T28715	2193-2195	NN	denotes	mr
T28717	2195-2196	HYPH	denotes	-
T28716	2196-2197	NN	denotes	s
T28718	2198-2208	NN	denotes	nucleotide
T28719	2209-2212	CC	denotes	and
T28720	2213-2218	NN	denotes	amino
T28721	2219-2224	NNS	denotes	acids
T28713	2225-2234	NNS	denotes	sequences
T28722	2234-2235	.	denotes	.
T28723	2235-2350	sentence	denotes	Boxed amino acids are the SAM domain sequence and the dashed box indicates a putative nuclear localization signal.
T28724	2236-2241	VBN	denotes	Boxed
T28726	2242-2247	NN	denotes	amino
T28725	2248-2253	NNS	denotes	acids
T28727	2254-2257	VBP	denotes	are
T28728	2258-2261	DT	denotes	the
T28730	2262-2265	NN	denotes	SAM
T28731	2266-2272	NN	denotes	domain
T28729	2273-2281	NN	denotes	sequence
T28732	2282-2285	CC	denotes	and
T28733	2286-2289	DT	denotes	the
T28735	2290-2296	JJ	denotes	dashed
T28734	2297-2300	NN	denotes	box
T28736	2301-2310	VBZ	denotes	indicates
T28737	2311-2312	DT	denotes	a
T28739	2313-2321	JJ	denotes	putative
T28740	2322-2329	JJ	denotes	nuclear
T28741	2330-2342	NN	denotes	localization
T28738	2343-2349	NN	denotes	signal
T28742	2349-2350	.	denotes	.
T28743	2350-2421	sentence	denotes	The underline indicates a putative polyadenylation termination signal.
T28744	2351-2354	DT	denotes	The
T28745	2355-2364	NN	denotes	underline
T28746	2365-2374	VBZ	denotes	indicates
T28747	2375-2376	DT	denotes	a
T28749	2377-2385	JJ	denotes	putative
T28750	2386-2401	NN	denotes	polyadenylation
T28751	2402-2413	NN	denotes	termination
T28748	2414-2420	NN	denotes	signal
T28752	2420-2421	.	denotes	.
T28753	2421-2495	sentence	denotes	(B) Alignment of SAM domain sequences for SAM domain-containing proteins.
T28754	2422-2423	-LRB-	denotes	(
T28755	2423-2424	LS	denotes	B
T28757	2424-2425	-RRB-	denotes	)
T28756	2426-2435	NN	denotes	Alignment
T28758	2436-2438	IN	denotes	of
T28759	2439-2442	NN	denotes	SAM
T28760	2443-2449	NN	denotes	domain
T28761	2450-2459	NNS	denotes	sequences
T28762	2460-2463	IN	denotes	for
T28763	2464-2467	NN	denotes	SAM
T28764	2468-2474	NN	denotes	domain
T28766	2474-2475	HYPH	denotes	-
T28765	2475-2485	VBG	denotes	containing
T28767	2486-2494	NN	denotes	proteins
T28768	2494-2495	.	denotes	.
T28769	2495-2536	sentence	denotes	The five alpha helices are marked H1-H5.
T28770	2496-2499	DT	denotes	The
T28772	2500-2504	CD	denotes	five
T28773	2505-2510	NN	denotes	alpha
T28771	2511-2518	NNS	denotes	helices
T28775	2519-2522	VBP	denotes	are
T28774	2523-2529	VBN	denotes	marked
T28776	2530-2532	NN	denotes	H1
T28777	2532-2533	SYM	denotes	-
T28778	2533-2535	NN	denotes	H5
T28779	2535-2536	.	denotes	.
T28780	2536-2660	sentence	denotes	Conserved amino acid residues are shown with a dark shadow and functionally similar residues are shown with a light shadow.
T28781	2537-2546	VBN	denotes	Conserved
T28783	2547-2552	NN	denotes	amino
T28784	2553-2557	NN	denotes	acid
T28782	2558-2566	NNS	denotes	residues
T28786	2567-2570	VBP	denotes	are
T28785	2571-2576	VBN	denotes	shown
T28787	2577-2581	IN	denotes	with
T28788	2582-2583	DT	denotes	a
T28790	2584-2588	JJ	denotes	dark
T28789	2589-2595	NN	denotes	shadow
T28791	2596-2599	CC	denotes	and
T28792	2600-2612	RB	denotes	functionally
T28793	2613-2620	JJ	denotes	similar
T28794	2621-2629	NNS	denotes	residues
T28796	2630-2633	VBP	denotes	are
T28795	2634-2639	VBN	denotes	shown
T28797	2640-2644	IN	denotes	with
T28798	2645-2646	DT	denotes	a
T28800	2647-2652	JJ	denotes	light
T28799	2653-2659	NN	denotes	shadow
T28801	2659-2660	.	denotes	.
T28802	2660-2730	sentence	denotes	The sites that were targeted for mutagenesis are indicated by arrows.
T28803	2661-2664	DT	denotes	The
T28804	2665-2670	NNS	denotes	sites
T28806	2671-2675	WDT	denotes	that
T28808	2676-2680	VBD	denotes	were
T28807	2681-2689	VBN	denotes	targeted
T28809	2690-2693	IN	denotes	for
T28810	2694-2705	NN	denotes	mutagenesis
T28811	2706-2709	VBP	denotes	are
T28805	2710-2719	VBN	denotes	indicated
T28812	2720-2722	IN	denotes	by
T28813	2723-2729	NNS	denotes	arrows
T28814	2729-2730	.	denotes	.
T28815	2730-2787	sentence	denotes	(C) Phylogenetic tree of SAM domain-containing proteins.
T28816	2731-2732	-LRB-	denotes	(
T28817	2732-2733	LS	denotes	C
T28819	2733-2734	-RRB-	denotes	)
T28820	2735-2747	JJ	denotes	Phylogenetic
T28818	2748-2752	NN	denotes	tree
T28821	2753-2755	IN	denotes	of
T28822	2756-2759	NN	denotes	SAM
T28823	2760-2766	NN	denotes	domain
T28825	2766-2767	HYPH	denotes	-
T28824	2767-2777	VBG	denotes	containing
T28826	2778-2786	NN	denotes	proteins
T28827	2786-2787	.	denotes	.
T28828	2787-2871	sentence	denotes	Amino acid sequences were analyzed by the neighbor-joining method in MacVector 7.2.
T28829	2788-2793	NN	denotes	Amino
T28830	2794-2798	NN	denotes	acid
T28831	2799-2808	NNS	denotes	sequences
T28833	2809-2813	VBD	denotes	were
T28832	2814-2822	VBN	denotes	analyzed
T28834	2823-2825	IN	denotes	by
T28835	2826-2829	DT	denotes	the
T28837	2830-2838	NN	denotes	neighbor
T28839	2838-2839	HYPH	denotes	-
T28838	2839-2846	VBG	denotes	joining
T28836	2847-2853	NN	denotes	method
T28840	2854-2856	IN	denotes	in
T28841	2857-2866	NNP	denotes	MacVector
T28842	2867-2870	CD	denotes	7.2
T28843	2870-2871	.	denotes	.
T28844	2871-2937	sentence	denotes	Branch lengths reflect the mean number of substitutions per site.
T28845	2872-2878	NN	denotes	Branch
T28846	2879-2886	NNS	denotes	lengths
T28847	2887-2894	VBP	denotes	reflect
T28848	2895-2898	DT	denotes	the
T28850	2899-2903	NN	denotes	mean
T28849	2904-2910	NN	denotes	number
T28851	2911-2913	IN	denotes	of
T28852	2914-2927	NNS	denotes	substitutions
T28853	2928-2931	IN	denotes	per
T28854	2932-2936	NN	denotes	site
T28855	2936-2937	.	denotes	.
T28856	2937-3048	sentence	denotes	(D) Schematic comparison of the amino acid sequences for mouse, rat, human, chick and zebrafish mr-s proteins.
T28857	2938-2939	-LRB-	denotes	(
T28858	2939-2940	LS	denotes	D
T28860	2940-2941	-RRB-	denotes	)
T28861	2942-2951	JJ	denotes	Schematic
T28859	2952-2962	NN	denotes	comparison
T28862	2963-2965	IN	denotes	of
T28863	2966-2969	DT	denotes	the
T28865	2970-2975	NN	denotes	amino
T28866	2976-2980	NN	denotes	acid
T28864	2981-2990	NNS	denotes	sequences
T28867	2991-2994	IN	denotes	for
T28868	2995-3000	NN	denotes	mouse
T28870	3000-3002	,	denotes	,
T28871	3002-3005	NN	denotes	rat
T28872	3005-3007	,	denotes	,
T28873	3007-3012	JJ	denotes	human
T28874	3012-3014	,	denotes	,
T28875	3014-3019	NN	denotes	chick
T28876	3020-3023	CC	denotes	and
T28877	3024-3033	NN	denotes	zebrafish
T28878	3034-3036	NN	denotes	mr
T28880	3036-3037	HYPH	denotes	-
T28879	3037-3038	NN	denotes	s
T28869	3039-3047	NN	denotes	proteins
T28881	3047-3048	.	denotes	.
T28882	3048-3168	sentence	denotes	The percent similarity of the SAM domains and other regions to the corresponding regions of the mouse protein is shown.
T28883	3049-3052	DT	denotes	The
T28885	3053-3060	NN	denotes	percent
T28884	3061-3071	NN	denotes	similarity
T28887	3072-3074	IN	denotes	of
T28888	3075-3078	DT	denotes	the
T28890	3079-3082	NN	denotes	SAM
T28889	3083-3090	NNS	denotes	domains
T28891	3091-3094	CC	denotes	and
T28892	3095-3100	JJ	denotes	other
T28893	3101-3108	NNS	denotes	regions
T28894	3109-3111	IN	denotes	to
T28895	3112-3115	DT	denotes	the
T28897	3116-3129	VBG	denotes	corresponding
T28896	3130-3137	NNS	denotes	regions
T28898	3138-3140	IN	denotes	of
T28899	3141-3144	DT	denotes	the
T28901	3145-3150	NN	denotes	mouse
T28900	3151-3158	NN	denotes	protein
T28902	3159-3161	VBZ	denotes	is
T28886	3162-3167	VBN	denotes	shown
T28903	3167-3168	.	denotes	.
T28904	3168-3242	sentence	denotes	Overall sequence similarity with the mouse protein is shown on the right.
T28905	3169-3176	JJ	denotes	Overall
T28907	3177-3185	NN	denotes	sequence
T28906	3186-3196	NN	denotes	similarity
T28909	3197-3201	IN	denotes	with
T28910	3202-3205	DT	denotes	the
T28912	3206-3211	NN	denotes	mouse
T28911	3212-3219	NN	denotes	protein
T28913	3220-3222	VBZ	denotes	is
T28908	3223-3228	VBN	denotes	shown
T28914	3229-3231	IN	denotes	on
T28915	3232-3235	DT	denotes	the
T28916	3236-3241	NN	denotes	right
T28917	3241-3242	.	denotes	.
T7365	3244-3254	NN	denotes	Expression
T7366	3255-3257	IN	denotes	of
T7367	3258-3260	NN	denotes	mr
T7369	3260-3261	HYPH	denotes	-
T7368	3261-3262	NN	denotes	s
T7370	3263-3265	IN	denotes	in
T7371	3266-3269	DT	denotes	the
T7373	3270-3280	VBG	denotes	developing
T7372	3281-3287	NN	denotes	retina
T7374	3288-3291	CC	denotes	and
T7375	3292-3295	DT	denotes	the
T7377	3296-3302	JJ	denotes	pineal
T7376	3303-3308	NN	denotes	gland
T7378	3308-3411	sentence	denotes	To investigate mr-s expression, we first performed whole mount in situ hybridization in mouse embryos.
T7379	3309-3311	TO	denotes	To
T7380	3312-3323	VB	denotes	investigate
T7382	3324-3326	NN	denotes	mr
T7384	3326-3327	HYPH	denotes	-
T7383	3327-3328	NN	denotes	s
T7385	3329-3339	NN	denotes	expression
T7386	3339-3341	,	denotes	,
T7387	3341-3343	PRP	denotes	we
T7388	3344-3349	RB	denotes	first
T7381	3350-3359	VBD	denotes	performed
T7389	3360-3365	JJ	denotes	whole
T7390	3366-3371	NN	denotes	mount
T7392	3372-3374	FW	denotes	in
T7393	3375-3379	FW	denotes	situ
T7391	3380-3393	NN	denotes	hybridization
T7394	3394-3396	IN	denotes	in
T7395	3397-3402	NN	denotes	mouse
T7396	3403-3410	NNS	denotes	embryos
T7397	3410-3411	.	denotes	.
T7398	3411-3510	sentence	denotes	No hybridization signal was detected at E9.5, E10.5 and E11.5 with an mr-s probe (data not shown).
T7399	3412-3414	DT	denotes	No
T7401	3415-3428	NN	denotes	hybridization
T7400	3429-3435	NN	denotes	signal
T7403	3436-3439	VBD	denotes	was
T7402	3440-3448	VBN	denotes	detected
T7404	3449-3451	IN	denotes	at
T7405	3452-3456	NN	denotes	E9.5
T7406	3456-3458	,	denotes	,
T7407	3458-3463	NN	denotes	E10.5
T7408	3464-3467	CC	denotes	and
T7409	3468-3473	NN	denotes	E11.5
T7410	3474-3478	IN	denotes	with
T7411	3479-3481	DT	denotes	an
T7413	3482-3484	NN	denotes	mr
T7415	3484-3485	HYPH	denotes	-
T7414	3485-3486	NN	denotes	s
T7412	3487-3492	NN	denotes	probe
T7416	3493-3494	-LRB-	denotes	(
T7418	3494-3498	NNS	denotes	data
T7419	3499-3502	RB	denotes	not
T7417	3503-3508	VBN	denotes	shown
T7420	3508-3509	-RRB-	denotes	)
T7421	3509-3510	.	denotes	.
T7422	3510-3634	sentence	denotes	We then investigated the expression of the mr-s gene in the developing retina by section in situ hybridization (Fig. 1A–G).
T7423	3511-3513	PRP	denotes	We
T7425	3514-3518	RB	denotes	then
T7424	3519-3531	VBD	denotes	investigated
T7426	3532-3535	DT	denotes	the
T7427	3536-3546	NN	denotes	expression
T7428	3547-3549	IN	denotes	of
T7429	3550-3553	DT	denotes	the
T7431	3554-3556	NN	denotes	mr
T7433	3556-3557	HYPH	denotes	-
T7432	3557-3558	NN	denotes	s
T7430	3559-3563	NN	denotes	gene
T7434	3564-3566	IN	denotes	in
T7435	3567-3570	DT	denotes	the
T7437	3571-3581	VBG	denotes	developing
T7436	3582-3588	NN	denotes	retina
T7438	3589-3591	IN	denotes	by
T7439	3592-3599	NN	denotes	section
T7441	3600-3602	FW	denotes	in
T7442	3603-3607	FW	denotes	situ
T7440	3608-3621	NN	denotes	hybridization
T7443	3622-3623	-LRB-	denotes	(
T7445	3623-3627	NN	denotes	Fig.
T7444	3628-3630	NN	denotes	1A
T7446	3630-3631	SYM	denotes	–
T7447	3631-3632	NN	denotes	G
T7448	3632-3633	-RRB-	denotes	)
T7449	3633-3634	.	denotes	.
T7450	3634-3712	sentence	denotes	No significant signal was detected in the developing retina at E13 (Fig. 2A).
T7451	3635-3637	DT	denotes	No
T7453	3638-3649	JJ	denotes	significant
T7452	3650-3656	NN	denotes	signal
T7455	3657-3660	VBD	denotes	was
T7454	3661-3669	VBN	denotes	detected
T7456	3670-3672	IN	denotes	in
T7457	3673-3676	DT	denotes	the
T7459	3677-3687	VBG	denotes	developing
T7458	3688-3694	NN	denotes	retina
T7460	3695-3697	IN	denotes	at
T7461	3698-3701	NN	denotes	E13
T7462	3702-3703	-LRB-	denotes	(
T7464	3703-3707	NN	denotes	Fig.
T7463	3708-3710	NN	denotes	2A
T7465	3710-3711	-RRB-	denotes	)
T7466	3711-3712	.	denotes	.
T7467	3712-3861	sentence	denotes	A weak signal was initially detected in the outer aspect of the neuroblastic layer (NBL), a presumptive photoreceptor layer at E18 (Fig. 2B, arrow).
T7468	3713-3714	DT	denotes	A
T7470	3715-3719	JJ	denotes	weak
T7469	3720-3726	NN	denotes	signal
T7472	3727-3730	VBD	denotes	was
T7473	3731-3740	RB	denotes	initially
T7471	3741-3749	VBN	denotes	detected
T7474	3750-3752	IN	denotes	in
T7475	3753-3756	DT	denotes	the
T7477	3757-3762	JJ	denotes	outer
T7476	3763-3769	NN	denotes	aspect
T7478	3770-3772	IN	denotes	of
T7479	3773-3776	DT	denotes	the
T7481	3777-3789	JJ	denotes	neuroblastic
T7480	3790-3795	NN	denotes	layer
T7482	3796-3797	-LRB-	denotes	(
T7483	3797-3800	NN	denotes	NBL
T7484	3800-3801	-RRB-	denotes	)
T7485	3801-3803	,	denotes	,
T7486	3803-3804	DT	denotes	a
T7488	3805-3816	JJ	denotes	presumptive
T7489	3817-3830	NN	denotes	photoreceptor
T7487	3831-3836	NN	denotes	layer
T7490	3837-3839	IN	denotes	at
T7491	3840-3843	NN	denotes	E18
T7492	3844-3845	-LRB-	denotes	(
T7494	3845-3849	NN	denotes	Fig.
T7493	3850-3852	NN	denotes	2B
T7495	3852-3854	,	denotes	,
T7496	3854-3859	NN	denotes	arrow
T7497	3859-3860	-RRB-	denotes	)
T7498	3860-3861	.	denotes	.
T7499	3861-3957	sentence	denotes	At P3, an mr-s transcript was clearly detected in the developing photoreceptor layer (Fig. 2E).
T7500	3862-3864	IN	denotes	At
T7502	3865-3867	NN	denotes	P3
T7503	3867-3869	,	denotes	,
T7504	3869-3871	DT	denotes	an
T7506	3872-3874	NN	denotes	mr
T7508	3874-3875	HYPH	denotes	-
T7507	3875-3876	NN	denotes	s
T7505	3877-3887	NN	denotes	transcript
T7509	3888-3891	VBD	denotes	was
T7510	3892-3899	RB	denotes	clearly
T7501	3900-3908	VBN	denotes	detected
T7511	3909-3911	IN	denotes	in
T7512	3912-3915	DT	denotes	the
T7514	3916-3926	VBG	denotes	developing
T7515	3927-3940	NN	denotes	photoreceptor
T7513	3941-3946	NN	denotes	layer
T7516	3947-3948	-LRB-	denotes	(
T7518	3948-3952	NN	denotes	Fig.
T7517	3953-3955	NN	denotes	2E
T7519	3955-3956	-RRB-	denotes	)
T7520	3956-3957	.	denotes	.
T7521	3957-4030	sentence	denotes	At P6, mr-s showed peak expression in the photoreceptor layer (Fig. 2F).
T7522	3958-3960	IN	denotes	At
T7524	3961-3963	NN	denotes	P6
T7525	3963-3965	,	denotes	,
T7526	3965-3967	NN	denotes	mr
T7528	3967-3968	HYPH	denotes	-
T7527	3968-3969	NN	denotes	s
T7523	3970-3976	VBD	denotes	showed
T7529	3977-3981	NN	denotes	peak
T7530	3982-3992	NN	denotes	expression
T7531	3993-3995	IN	denotes	in
T7532	3996-3999	DT	denotes	the
T7534	4000-4013	NN	denotes	photoreceptor
T7533	4014-4019	NN	denotes	layer
T7535	4020-4021	-LRB-	denotes	(
T7537	4021-4025	NN	denotes	Fig.
T7536	4026-4028	NN	denotes	2F
T7538	4028-4029	-RRB-	denotes	)
T7539	4029-4030	.	denotes	.
T7540	4030-4165	sentence	denotes	This pattern correlates with the rapid increase in cells expressing rhodopsin and other phototransduction genes between P6-P8 [34-37].
T7541	4031-4035	DT	denotes	This
T7542	4036-4043	NN	denotes	pattern
T7543	4044-4054	VBZ	denotes	correlates
T7544	4055-4059	IN	denotes	with
T7545	4060-4063	DT	denotes	the
T7547	4064-4069	JJ	denotes	rapid
T7546	4070-4078	NN	denotes	increase
T7548	4079-4081	IN	denotes	in
T7549	4082-4087	NNS	denotes	cells
T7550	4088-4098	VBG	denotes	expressing
T7551	4099-4108	NN	denotes	rhodopsin
T7552	4109-4112	CC	denotes	and
T7553	4113-4118	JJ	denotes	other
T7555	4119-4136	NN	denotes	phototransduction
T7554	4137-4142	NNS	denotes	genes
T7556	4143-4150	IN	denotes	between
T7557	4151-4153	NN	denotes	P6
T7558	4153-4154	SYM	denotes	-
T7559	4154-4156	NN	denotes	P8
T7560	4157-4158	-LRB-	denotes	[
T7561	4158-4160	CD	denotes	34
T7562	4160-4161	SYM	denotes	-
T7563	4161-4163	CD	denotes	37
T7564	4163-4164	-RRB-	denotes	]
T7565	4164-4165	.	denotes	.
T7566	4165-4300	sentence	denotes	At P9, the intensity of the mr-s signal was significantly reduced but was localized to the outer nuclear layer (ONL) (data not shown).
T7567	4166-4168	IN	denotes	At
T7569	4169-4171	NN	denotes	P9
T7570	4171-4173	,	denotes	,
T7571	4173-4176	DT	denotes	the
T7572	4177-4186	NN	denotes	intensity
T7573	4187-4189	IN	denotes	of
T7574	4190-4193	DT	denotes	the
T7576	4194-4196	NN	denotes	mr
T7578	4196-4197	HYPH	denotes	-
T7577	4197-4198	NN	denotes	s
T7575	4199-4205	NN	denotes	signal
T7579	4206-4209	VBD	denotes	was
T7580	4210-4223	RB	denotes	significantly
T7568	4224-4231	VBN	denotes	reduced
T7581	4232-4235	CC	denotes	but
T7582	4236-4239	VBD	denotes	was
T7583	4240-4249	VBN	denotes	localized
T7584	4250-4252	IN	denotes	to
T7585	4253-4256	DT	denotes	the
T7587	4257-4262	JJ	denotes	outer
T7588	4263-4270	JJ	denotes	nuclear
T7586	4271-4276	NN	denotes	layer
T7589	4277-4278	-LRB-	denotes	(
T7590	4278-4281	NN	denotes	ONL
T7591	4281-4282	-RRB-	denotes	)
T7592	4283-4284	-LRB-	denotes	(
T7594	4284-4288	NNS	denotes	data
T7595	4289-4292	RB	denotes	not
T7593	4293-4298	VBN	denotes	shown
T7596	4298-4299	-RRB-	denotes	)
T7597	4299-4300	.	denotes	.
T7598	4300-4399	sentence	denotes	Faint expression of mr-s mRNA was observed in mature photoreceptors in the adult retina (Fig. 2G).
T7599	4301-4306	NN	denotes	Faint
T7600	4307-4317	NN	denotes	expression
T7602	4318-4320	IN	denotes	of
T7603	4321-4323	NN	denotes	mr
T7605	4323-4324	HYPH	denotes	-
T7604	4324-4325	NN	denotes	s
T7606	4326-4330	NN	denotes	mRNA
T7607	4331-4334	VBD	denotes	was
T7601	4335-4343	VBN	denotes	observed
T7608	4344-4346	IN	denotes	in
T7609	4347-4353	JJ	denotes	mature
T7610	4354-4368	NNS	denotes	photoreceptors
T7611	4369-4371	IN	denotes	in
T7612	4372-4375	DT	denotes	the
T7614	4376-4381	JJ	denotes	adult
T7613	4382-4388	NN	denotes	retina
T7615	4389-4390	-LRB-	denotes	(
T7617	4390-4394	NN	denotes	Fig.
T7616	4395-4397	NN	denotes	2G
T7618	4397-4398	-RRB-	denotes	)
T7619	4398-4399	.	denotes	.
T7620	4399-4400	sentence	denotes
T29458	4410-4412	NN	denotes	mr
T29460	4412-4413	HYPH	denotes	-
T29459	4413-4414	NN	denotes	s
T29461	4415-4425	NN	denotes	expression
T29462	4426-4428	IN	denotes	in
T29463	4429-4439	VBG	denotes	developing
T29465	4440-4445	NN	denotes	mouse
T29464	4446-4452	NN	denotes	retina
T29466	4453-4456	CC	denotes	and
T29467	4457-4463	JJ	denotes	pineal
T29468	4464-4469	NN	denotes	gland
T29469	4469-4470	.	denotes	.
T29470	4470-4532	sentence	denotes	(A-G) mr-s expression during development of the mouse retina.
T29471	4471-4472	-LRB-	denotes	(
T29472	4472-4473	LS	denotes	A
T29474	4473-4474	SYM	denotes	-
T29475	4474-4475	LS	denotes	G
T29476	4475-4476	-RRB-	denotes	)
T29477	4477-4479	NN	denotes	mr
T29479	4479-4480	HYPH	denotes	-
T29478	4480-4481	NN	denotes	s
T29473	4482-4492	NN	denotes	expression
T29480	4493-4499	IN	denotes	during
T29481	4500-4511	NN	denotes	development
T29482	4512-4514	IN	denotes	of
T29483	4515-4518	DT	denotes	the
T29485	4519-4524	NN	denotes	mouse
T29484	4525-4531	NN	denotes	retina
T29486	4531-4532	.	denotes	.
T29487	4532-4602	sentence	denotes	The in situ hybridization signal of mr-s was not detected at E13 (A).
T29488	4533-4536	DT	denotes	The
T29490	4537-4539	FW	denotes	in
T29491	4540-4544	FW	denotes	situ
T29492	4545-4558	NN	denotes	hybridization
T29489	4559-4565	NN	denotes	signal
T29494	4566-4568	IN	denotes	of
T29495	4569-4571	NN	denotes	mr
T29497	4571-4572	HYPH	denotes	-
T29496	4572-4573	NN	denotes	s
T29498	4574-4577	VBD	denotes	was
T29499	4578-4581	RB	denotes	not
T29493	4582-4590	VBN	denotes	detected
T29500	4591-4593	IN	denotes	at
T29501	4594-4597	NN	denotes	E13
T29502	4598-4599	-LRB-	denotes	(
T29503	4599-4600	NN	denotes	A
T29504	4600-4601	-RRB-	denotes	)
T29505	4601-4602	.	denotes	.
T29506	4602-4679	sentence	denotes	The signal (arrow) was first detected in the outer aspect of NBL at E18 (B).
T29507	4603-4606	DT	denotes	The
T29508	4607-4613	NN	denotes	signal
T29510	4614-4615	-LRB-	denotes	(
T29511	4615-4620	NN	denotes	arrow
T29512	4620-4621	-RRB-	denotes	)
T29513	4622-4625	VBD	denotes	was
T29514	4626-4631	RB	denotes	first
T29509	4632-4640	VBN	denotes	detected
T29515	4641-4643	IN	denotes	in
T29516	4644-4647	DT	denotes	the
T29518	4648-4653	JJ	denotes	outer
T29517	4654-4660	NN	denotes	aspect
T29519	4661-4663	IN	denotes	of
T29520	4664-4667	NN	denotes	NBL
T29521	4668-4670	IN	denotes	at
T29522	4671-4674	NN	denotes	E18
T29523	4675-4676	-LRB-	denotes	(
T29524	4676-4677	NN	denotes	B
T29525	4677-4678	-RRB-	denotes	)
T29526	4678-4679	.	denotes	.
T29527	4679-4809	sentence	denotes	A strong mr-s signal was detected in outer layer of the retina at P3-P6, and then the signal decreased in the adult retina (E-G).
T29528	4680-4681	DT	denotes	A
T29530	4682-4688	JJ	denotes	strong
T29531	4689-4691	NN	denotes	mr
T29533	4691-4692	HYPH	denotes	-
T29532	4692-4693	NN	denotes	s
T29529	4694-4700	NN	denotes	signal
T29535	4701-4704	VBD	denotes	was
T29534	4705-4713	VBN	denotes	detected
T29536	4714-4716	IN	denotes	in
T29537	4717-4722	JJ	denotes	outer
T29538	4723-4728	NN	denotes	layer
T29539	4729-4731	IN	denotes	of
T29540	4732-4735	DT	denotes	the
T29541	4736-4742	NN	denotes	retina
T29542	4743-4745	IN	denotes	at
T29543	4746-4748	NN	denotes	P3
T29544	4748-4749	SYM	denotes	-
T29545	4749-4751	NN	denotes	P6
T29546	4751-4753	,	denotes	,
T29547	4753-4756	CC	denotes	and
T29548	4757-4761	RB	denotes	then
T29550	4762-4765	DT	denotes	the
T29551	4766-4772	NN	denotes	signal
T29549	4773-4782	VBD	denotes	decreased
T29552	4783-4785	IN	denotes	in
T29553	4786-4789	DT	denotes	the
T29555	4790-4795	JJ	denotes	adult
T29554	4796-4802	NN	denotes	retina
T29556	4803-4804	-LRB-	denotes	(
T29557	4804-4805	NN	denotes	E
T29558	4805-4806	SYM	denotes	-
T29559	4806-4807	NN	denotes	G
T29560	4807-4808	-RRB-	denotes	)
T29561	4808-4809	.	denotes	.
T29562	4809-4866	sentence	denotes	Control with the sense probe in E18 retina is shown (C).
T29563	4810-4817	NN	denotes	Control
T29565	4818-4822	IN	denotes	with
T29566	4823-4826	DT	denotes	the
T29568	4827-4832	NN	denotes	sense
T29567	4833-4838	NN	denotes	probe
T29569	4839-4841	IN	denotes	in
T29570	4842-4845	NN	denotes	E18
T29571	4846-4852	NN	denotes	retina
T29572	4853-4855	VBZ	denotes	is
T29564	4856-4861	VBN	denotes	shown
T29573	4862-4863	-LRB-	denotes	(
T29574	4863-4864	NN	denotes	C
T29575	4864-4865	-RRB-	denotes	)
T29576	4865-4866	.	denotes	.
T29577	4866-4885	sentence	denotes	Scale bar, 100 μm.
T29578	4867-4872	NN	denotes	Scale
T29579	4873-4876	NN	denotes	bar
T29580	4876-4878	,	denotes	,
T29581	4878-4881	CD	denotes	100
T29582	4882-4884	NN	denotes	μm
T29583	4884-4885	.	denotes	.
T29584	4885-4954	sentence	denotes	(H) Northern blot analysis of mr-s expression in adult mouse organs.
T29585	4886-4887	-LRB-	denotes	(
T29586	4887-4888	LS	denotes	H
T29588	4888-4889	-RRB-	denotes	)
T29589	4890-4898	NNP	denotes	Northern
T29590	4899-4903	NN	denotes	blot
T29587	4904-4912	NN	denotes	analysis
T29591	4913-4915	IN	denotes	of
T29592	4916-4918	NN	denotes	mr
T29594	4918-4919	HYPH	denotes	-
T29593	4919-4920	NN	denotes	s
T29595	4921-4931	NN	denotes	expression
T29596	4932-4934	IN	denotes	in
T29597	4935-4940	JJ	denotes	adult
T29599	4941-4946	NN	denotes	mouse
T29598	4947-4953	NNS	denotes	organs
T29600	4953-4954	.	denotes	.
T29601	4954-5002	sentence	denotes	The arrow corresponds to 2.2kb mr-s transcript.
T29602	4955-4958	DT	denotes	The
T29603	4959-4964	NN	denotes	arrow
T29604	4965-4976	VBZ	denotes	corresponds
T29605	4977-4979	IN	denotes	to
T29606	4980-4985	NN	denotes	2.2kb
T29608	4986-4988	NN	denotes	mr
T29610	4988-4989	HYPH	denotes	-
T29609	4989-4990	NN	denotes	s
T29607	4991-5001	NN	denotes	transcript
T29611	5001-5002	.	denotes	.
T29612	5002-5230	sentence	denotes	(I) RT-PCR analysis of total RNAs extracted from E13 whole embryo, P0 whole body (except for the eye), P7 retina, P7 pineal gland, P7 brain, P7 liver, adult retina, adult pineal gland, adult brain and adult liver, respectively.
T29613	5003-5004	-LRB-	denotes	(
T29614	5004-5005	LS	denotes	I
T29616	5005-5006	-RRB-	denotes	)
T29617	5007-5009	NN	denotes	RT
T29619	5009-5010	HYPH	denotes	-
T29618	5010-5013	NN	denotes	PCR
T29615	5014-5022	NN	denotes	analysis
T29620	5023-5025	IN	denotes	of
T29621	5026-5031	JJ	denotes	total
T29622	5032-5036	NNS	denotes	RNAs
T29623	5037-5046	VBN	denotes	extracted
T29624	5047-5051	IN	denotes	from
T29625	5052-5055	NN	denotes	E13
T29627	5056-5061	JJ	denotes	whole
T29626	5062-5068	NN	denotes	embryo
T29628	5068-5070	,	denotes	,
T29629	5070-5072	NN	denotes	P0
T29631	5073-5078	JJ	denotes	whole
T29630	5079-5083	NN	denotes	body
T29632	5084-5085	-LRB-	denotes	(
T29633	5085-5091	IN	denotes	except
T29634	5092-5095	IN	denotes	for
T29635	5096-5099	DT	denotes	the
T29636	5100-5103	NN	denotes	eye
T29637	5103-5104	-RRB-	denotes	)
T29638	5104-5106	,	denotes	,
T29639	5106-5108	NN	denotes	P7
T29640	5109-5115	NN	denotes	retina
T29641	5115-5117	,	denotes	,
T29642	5117-5119	NN	denotes	P7
T29644	5120-5126	JJ	denotes	pineal
T29643	5127-5132	NN	denotes	gland
T29645	5132-5134	,	denotes	,
T29646	5134-5136	NN	denotes	P7
T29647	5137-5142	NN	denotes	brain
T29648	5142-5144	,	denotes	,
T29649	5144-5146	NN	denotes	P7
T29650	5147-5152	NN	denotes	liver
T29651	5152-5154	,	denotes	,
T29652	5154-5159	JJ	denotes	adult
T29653	5160-5166	NN	denotes	retina
T29654	5166-5168	,	denotes	,
T29655	5168-5173	JJ	denotes	adult
T29657	5174-5180	JJ	denotes	pineal
T29656	5181-5186	NN	denotes	gland
T29658	5186-5188	,	denotes	,
T29659	5188-5193	JJ	denotes	adult
T29660	5194-5199	NN	denotes	brain
T29661	5200-5203	CC	denotes	and
T29662	5204-5209	JJ	denotes	adult
T29663	5210-5215	NN	denotes	liver
T29664	5215-5217	,	denotes	,
T29665	5217-5229	RB	denotes	respectively
T29666	5229-5230	.	denotes	.
T29667	5230-5385	sentence	denotes	RPE, retinal pigment epithelium; NR, neural retina; NBL, neuroblastic layer; GCL, ganglion cell layer; ONL, outer nuclear layer; INL, inner nuclear layer.
T29668	5231-5234	NN	denotes	RPE
T29669	5234-5236	,	denotes	,
T29670	5236-5243	JJ	denotes	retinal
T29672	5244-5251	NN	denotes	pigment
T29671	5252-5262	NN	denotes	epithelium
T29673	5262-5263	:	denotes	;
T29674	5264-5266	NN	denotes	NR
T29675	5266-5268	,	denotes	,
T29676	5268-5274	JJ	denotes	neural
T29677	5275-5281	NN	denotes	retina
T29678	5281-5282	:	denotes	;
T29679	5283-5286	NN	denotes	NBL
T29680	5286-5288	,	denotes	,
T29681	5288-5300	JJ	denotes	neuroblastic
T29682	5301-5306	NN	denotes	layer
T29683	5306-5307	:	denotes	;
T29684	5308-5311	NN	denotes	GCL
T29685	5311-5313	,	denotes	,
T29686	5313-5321	NN	denotes	ganglion
T29687	5322-5326	NN	denotes	cell
T29688	5327-5332	NN	denotes	layer
T29689	5332-5333	:	denotes	;
T29690	5334-5337	NN	denotes	ONL
T29691	5337-5339	,	denotes	,
T29692	5339-5344	JJ	denotes	outer
T29694	5345-5352	JJ	denotes	nuclear
T29693	5353-5358	NN	denotes	layer
T29695	5358-5359	:	denotes	;
T29696	5360-5363	NN	denotes	INL
T29697	5363-5365	,	denotes	,
T29698	5365-5370	JJ	denotes	inner
T29700	5371-5378	JJ	denotes	nuclear
T29699	5379-5384	NN	denotes	layer
T29701	5384-5385	.	denotes	.
T7622	5386-5387	DT	denotes	T
T7621	5386-5393	sentence	denotes	To dete
T7623	5387-5388	NN	denotes	o
T7624	5389-5393	VBN	denotes	dete
T7625	5393-5394	sentence	denotes	r
T7626	5393-5394	NN	denotes	r
T7629	5394-5395	NN	denotes	m
T7627	5394-5410	sentence	denotes	mine the tissue
T7628	5395-5397	NN	denotes	in
T7630	5397-5398	VBN	denotes	e
T7631	5399-5400	JJ	denotes	t
T7632	5400-5402	DT	denotes	he
T7633	5403-5405	NN	denotes	ti
T7635	5405-5406	NN	denotes	s
T7634	5406-5407	VBD	denotes	s
T7637	5407-5408	JJ	denotes	u
T7636	5408-5409	NN	denotes	e
T7640	5410-5411	NN	denotes	s
T7638	5410-5413	sentence	denotes	spe
T7639	5411-5413	NN	denotes	pe
T7642	5413-5414	NN	denotes	c
T7641	5413-5414	sentence	denotes	c
T7644	5414-5415	NN	denotes	i
T7643	5414-5417	sentence	denotes	ifi
T7645	5415-5416	IN	denotes	f
T7646	5416-5417	NN	denotes	i
T7648	5417-5418	VBZ	denotes	c
T7647	5417-5420	sentence	denotes	cit
T7649	5418-5420	NN	denotes	it
T7651	5420-5421	NN	denotes	y
T7650	5420-5427	sentence	denotes	y of mr
T7652	5422-5424	IN	denotes	of
T7653	5425-5426	IN	denotes	m
T7654	5426-5427	NN	denotes	r
T7658	5427-5428	HYPH	denotes	-
T7655	5427-5548	sentence	denotes	-s expression, the expression of the mr-s gene in various adult tissues was examined by Northern hybridization (Fig. 2H).
T7657	5428-5429	NN	denotes	s
T7659	5430-5440	NN	denotes	expression
T7660	5440-5442	,	denotes	,
T7661	5442-5445	DT	denotes	the
T7662	5446-5456	NN	denotes	expression
T7663	5457-5459	IN	denotes	of
T7664	5460-5463	DT	denotes	the
T7666	5464-5466	NN	denotes	mr
T7668	5466-5467	HYPH	denotes	-
T7667	5467-5468	NN	denotes	s
T7665	5469-5473	NN	denotes	gene
T7669	5474-5476	IN	denotes	in
T7670	5477-5484	JJ	denotes	various
T7672	5485-5490	JJ	denotes	adult
T7671	5491-5498	NNS	denotes	tissues
T7673	5499-5502	VBD	denotes	was
T7656	5503-5511	VBN	denotes	examined
T7674	5512-5514	IN	denotes	by
T7675	5515-5523	NNP	denotes	Northern
T7676	5524-5537	NN	denotes	hybridization
T7677	5538-5539	-LRB-	denotes	(
T7679	5539-5543	NN	denotes	Fig.
T7678	5544-5546	NN	denotes	2H
T7680	5546-5547	-RRB-	denotes	)
T7681	5547-5548	.	denotes	.
T7682	5548-5587	sentence	denotes	As a control, P7 retinal RNA was used.
T7683	5549-5551	IN	denotes	As
T7685	5552-5553	DT	denotes	a
T7686	5554-5561	NN	denotes	control
T7687	5561-5563	,	denotes	,
T7688	5563-5565	NN	denotes	P7
T7690	5566-5573	NN	denotes	retinal
T7689	5574-5577	NN	denotes	RNA
T7691	5578-5581	VBD	denotes	was
T7684	5582-5586	VBN	denotes	used
T7692	5586-5587	.	denotes	.
T7693	5587-5677	sentence	denotes	Four bands corresponding to 7.2-kb, 4.0-kb, 2.5-kb and 2.2-kb were detected in P7 retina.
T7694	5588-5592	CD	denotes	Four
T7695	5593-5598	NNS	denotes	bands
T7697	5599-5612	VBG	denotes	corresponding
T7698	5613-5615	IN	denotes	to
T7699	5616-5619	CD	denotes	7.2
T7701	5619-5620	HYPH	denotes	-
T7700	5620-5622	NN	denotes	kb
T7702	5622-5624	,	denotes	,
T7703	5624-5627	CD	denotes	4.0
T7705	5627-5628	HYPH	denotes	-
T7704	5628-5630	NN	denotes	kb
T7706	5630-5632	,	denotes	,
T7707	5632-5635	CD	denotes	2.5
T7709	5635-5636	HYPH	denotes	-
T7708	5636-5638	NN	denotes	kb
T7710	5639-5642	CC	denotes	and
T7711	5643-5646	CD	denotes	2.2
T7713	5646-5647	HYPH	denotes	-
T7712	5647-5649	NN	denotes	kb
T7714	5650-5654	VBD	denotes	were
T7696	5655-5663	VBN	denotes	detected
T7715	5664-5666	IN	denotes	in
T7716	5667-5669	NN	denotes	P7
T7717	5670-5676	NN	denotes	retina
T7718	5676-5677	.	denotes	.
T7719	5677-5746	sentence	denotes	The 2.2-kb band corresponds to the cDNA characterized in this study.
T7720	5678-5681	DT	denotes	The
T7722	5682-5685	CD	denotes	2.2
T7724	5685-5686	HYPH	denotes	-
T7723	5686-5688	NN	denotes	kb
T7721	5689-5693	NN	denotes	band
T7725	5694-5705	VBZ	denotes	corresponds
T7726	5706-5708	IN	denotes	to
T7727	5709-5712	DT	denotes	the
T7728	5713-5717	NN	denotes	cDNA
T7729	5718-5731	VBN	denotes	characterized
T7730	5732-5734	IN	denotes	in
T7731	5735-5739	DT	denotes	this
T7732	5740-5745	NN	denotes	study
T7733	5745-5746	.	denotes	.
T7734	5746-5839	sentence	denotes	The larger bands, possibly alternative spliced transcripts, have not yet been characterized.
T7735	5747-5750	DT	denotes	The
T7737	5751-5757	JJR	denotes	larger
T7736	5758-5763	NNS	denotes	bands
T7739	5763-5765	,	denotes	,
T7740	5765-5773	RB	denotes	possibly
T7742	5774-5785	JJ	denotes	alternative
T7743	5786-5793	VBN	denotes	spliced
T7741	5794-5805	NNS	denotes	transcripts
T7744	5805-5807	,	denotes	,
T7745	5807-5811	VBP	denotes	have
T7746	5812-5815	RB	denotes	not
T7747	5816-5819	RB	denotes	yet
T7748	5820-5824	VBN	denotes	been
T7738	5825-5838	VBN	denotes	characterized
T7749	5838-5839	.	denotes	.
T7750	5839-6013	sentence	denotes	The mr-s probe did not detect a band in the adult tissues examined, indicating that these tissues do not express mr-s at a level comparable to that of the developing retina.
T7751	5840-5843	DT	denotes	The
T7753	5844-5846	NN	denotes	mr
T7755	5846-5847	HYPH	denotes	-
T7754	5847-5848	NN	denotes	s
T7752	5849-5854	NN	denotes	probe
T7757	5855-5858	VBD	denotes	did
T7758	5859-5862	RB	denotes	not
T7756	5863-5869	VB	denotes	detect
T7759	5870-5871	DT	denotes	a
T7760	5872-5876	NN	denotes	band
T7761	5877-5879	IN	denotes	in
T7762	5880-5883	DT	denotes	the
T7764	5884-5889	JJ	denotes	adult
T7763	5890-5897	NNS	denotes	tissues
T7765	5898-5906	VBN	denotes	examined
T7766	5906-5908	,	denotes	,
T7767	5908-5918	VBG	denotes	indicating
T7768	5919-5923	IN	denotes	that
T7770	5924-5929	DT	denotes	these
T7771	5930-5937	NNS	denotes	tissues
T7772	5938-5940	VBP	denotes	do
T7773	5941-5944	RB	denotes	not
T7769	5945-5952	VB	denotes	express
T7774	5953-5955	NN	denotes	mr
T7776	5955-5956	HYPH	denotes	-
T7775	5956-5957	NN	denotes	s
T7777	5958-5960	IN	denotes	at
T7778	5961-5962	DT	denotes	a
T7779	5963-5968	NN	denotes	level
T7780	5969-5979	JJ	denotes	comparable
T7781	5980-5982	IN	denotes	to
T7782	5983-5987	DT	denotes	that
T7783	5988-5990	IN	denotes	of
T7784	5991-5994	DT	denotes	the
T7786	5995-6005	VBG	denotes	developing
T7785	6006-6012	NN	denotes	retina
T7787	6012-6013	.	denotes	.
T7788	6013-6123	sentence	denotes	Previous reports revealed that many photoreceptor-specific genes are also expressed in the pineal gland [38].
T7789	6014-6022	JJ	denotes	Previous
T7790	6023-6030	NNS	denotes	reports
T7791	6031-6039	VBD	denotes	revealed
T7792	6040-6044	IN	denotes	that
T7794	6045-6049	JJ	denotes	many
T7796	6050-6063	NN	denotes	photoreceptor
T7798	6063-6064	HYPH	denotes	-
T7797	6064-6072	JJ	denotes	specific
T7795	6073-6078	NNS	denotes	genes
T7799	6079-6082	VBP	denotes	are
T7800	6083-6087	RB	denotes	also
T7793	6088-6097	VBN	denotes	expressed
T7801	6098-6100	IN	denotes	in
T7802	6101-6104	DT	denotes	the
T7804	6105-6111	JJ	denotes	pineal
T7803	6112-6117	NN	denotes	gland
T7805	6118-6119	-LRB-	denotes	[
T7806	6119-6121	CD	denotes	38
T7807	6121-6122	-RRB-	denotes	]
T7808	6122-6123	.	denotes	.
T7809	6123-6313	sentence	denotes	We examined the expression of mr-s transcripts in the whole embryo, whole body, retina, pineal gland, brain, liver and other organs at various stages by RT-PCR (Fig. 2I and data not shown).
T7810	6124-6126	PRP	denotes	We
T7811	6127-6135	VBD	denotes	examined
T7812	6136-6139	DT	denotes	the
T7813	6140-6150	NN	denotes	expression
T7814	6151-6153	IN	denotes	of
T7815	6154-6156	NN	denotes	mr
T7817	6156-6157	HYPH	denotes	-
T7816	6157-6158	NN	denotes	s
T7818	6159-6170	NNS	denotes	transcripts
T7819	6171-6173	IN	denotes	in
T7820	6174-6177	DT	denotes	the
T7822	6178-6183	JJ	denotes	whole
T7821	6184-6190	NN	denotes	embryo
T7823	6190-6192	,	denotes	,
T7824	6192-6197	JJ	denotes	whole
T7825	6198-6202	NN	denotes	body
T7826	6202-6204	,	denotes	,
T7827	6204-6210	NN	denotes	retina
T7828	6210-6212	,	denotes	,
T7829	6212-6218	JJ	denotes	pineal
T7830	6219-6224	NN	denotes	gland
T7831	6224-6226	,	denotes	,
T7832	6226-6231	NN	denotes	brain
T7833	6231-6233	,	denotes	,
T7834	6233-6238	NN	denotes	liver
T7835	6239-6242	CC	denotes	and
T7836	6243-6248	JJ	denotes	other
T7837	6249-6255	NNS	denotes	organs
T7838	6256-6258	IN	denotes	at
T7839	6259-6266	JJ	denotes	various
T7840	6267-6273	NNS	denotes	stages
T7841	6274-6276	IN	denotes	by
T7842	6277-6279	NN	denotes	RT
T7844	6279-6280	HYPH	denotes	-
T7843	6280-6283	NN	denotes	PCR
T7845	6284-6285	-LRB-	denotes	(
T7847	6285-6289	NN	denotes	Fig.
T7846	6290-6292	NN	denotes	2I
T7848	6293-6296	CC	denotes	and
T7849	6297-6301	NNS	denotes	data
T7851	6302-6305	RB	denotes	not
T7850	6306-6311	VBN	denotes	shown
T7852	6311-6312	-RRB-	denotes	)
T7853	6312-6313	.	denotes	.
T7854	6313-6434	sentence	denotes	We amplified PCR fragments of 292 bp and 452 bp with primer pairs for genes encoding mouse mr-s and G3PDH, respectively.
T7855	6314-6316	PRP	denotes	We
T7856	6317-6326	VBD	denotes	amplified
T7857	6327-6330	NN	denotes	PCR
T7858	6331-6340	NNS	denotes	fragments
T7859	6341-6343	IN	denotes	of
T7860	6344-6347	CD	denotes	292
T7861	6348-6350	NN	denotes	bp
T7862	6351-6354	CC	denotes	and
T7863	6355-6358	CD	denotes	452
T7864	6359-6361	NN	denotes	bp
T7865	6362-6366	IN	denotes	with
T7866	6367-6373	NN	denotes	primer
T7867	6374-6379	NNS	denotes	pairs
T7868	6380-6383	IN	denotes	for
T7869	6384-6389	NNS	denotes	genes
T7870	6390-6398	VBG	denotes	encoding
T7871	6399-6404	NN	denotes	mouse
T7873	6405-6407	NN	denotes	mr
T7874	6407-6408	HYPH	denotes	-
T7872	6408-6409	NN	denotes	s
T7875	6410-6413	CC	denotes	and
T7876	6414-6419	NN	denotes	G3PDH
T7877	6419-6421	,	denotes	,
T7878	6421-6433	RB	denotes	respectively
T7879	6433-6434	.	denotes	.
T7880	6434-6523	sentence	denotes	In E13 whole embryo and P0 whole body (except for the eye), no mr-s signal was detected.
T7881	6435-6437	IN	denotes	In
T7883	6438-6441	NN	denotes	E13
T7885	6442-6447	JJ	denotes	whole
T7884	6448-6454	NN	denotes	embryo
T7886	6455-6458	CC	denotes	and
T7887	6459-6461	NN	denotes	P0
T7889	6462-6467	JJ	denotes	whole
T7888	6468-6472	NN	denotes	body
T7890	6473-6474	-LRB-	denotes	(
T7891	6474-6480	IN	denotes	except
T7892	6481-6484	IN	denotes	for
T7893	6485-6488	DT	denotes	the
T7894	6489-6492	NN	denotes	eye
T7895	6492-6493	-RRB-	denotes	)
T7896	6493-6495	,	denotes	,
T7897	6495-6497	DT	denotes	no
T7899	6498-6500	NN	denotes	mr
T7901	6500-6501	HYPH	denotes	-
T7900	6501-6502	NN	denotes	s
T7898	6503-6509	NN	denotes	signal
T7902	6510-6513	VBD	denotes	was
T7882	6514-6522	VBN	denotes	detected
T7903	6522-6523	.	denotes	.
T7904	6523-6605	sentence	denotes	As expected, we observed that mr-s is expressed in the P7 and adult pineal gland.
T7905	6524-6526	IN	denotes	As
T7906	6527-6535	VBN	denotes	expected
T7908	6535-6537	,	denotes	,
T7909	6537-6539	PRP	denotes	we
T7907	6540-6548	VBD	denotes	observed
T7910	6549-6553	IN	denotes	that
T7912	6554-6556	NN	denotes	mr
T7914	6556-6557	HYPH	denotes	-
T7913	6557-6558	NN	denotes	s
T7915	6559-6561	VBZ	denotes	is
T7911	6562-6571	VBN	denotes	expressed
T7916	6572-6574	IN	denotes	in
T7917	6575-6578	DT	denotes	the
T7919	6579-6581	NN	denotes	P7
T7920	6582-6585	CC	denotes	and
T7921	6586-6591	JJ	denotes	adult
T7922	6592-6598	JJ	denotes	pineal
T7918	6599-6604	NN	denotes	gland
T7923	6604-6605	.	denotes	.
T7924	6605-6745	sentence	denotes	In the P7 and adult brain, liver and several other organs, the RT-PCR amplified band of mr-s was not detected (Fig. 2I and data not shown).
T7925	6606-6608	IN	denotes	In
T7927	6609-6612	DT	denotes	the
T7929	6613-6615	NN	denotes	P7
T7930	6616-6619	CC	denotes	and
T7931	6620-6625	JJ	denotes	adult
T7928	6626-6631	NN	denotes	brain
T7932	6631-6633	,	denotes	,
T7933	6633-6638	NN	denotes	liver
T7934	6639-6642	CC	denotes	and
T7935	6643-6650	JJ	denotes	several
T7937	6651-6656	JJ	denotes	other
T7936	6657-6663	NNS	denotes	organs
T7938	6663-6665	,	denotes	,
T7939	6665-6668	DT	denotes	the
T7941	6669-6671	NN	denotes	RT
T7943	6671-6672	HYPH	denotes	-
T7942	6672-6675	NN	denotes	PCR
T7944	6676-6685	VBN	denotes	amplified
T7940	6686-6690	NN	denotes	band
T7945	6691-6693	IN	denotes	of
T7946	6694-6696	NN	denotes	mr
T7948	6696-6697	HYPH	denotes	-
T7947	6697-6698	NN	denotes	s
T7949	6699-6702	VBD	denotes	was
T7950	6703-6706	RB	denotes	not
T7926	6707-6715	VBN	denotes	detected
T7951	6716-6717	-LRB-	denotes	(
T7953	6717-6721	NN	denotes	Fig.
T7952	6722-6724	NN	denotes	2I
T7954	6725-6728	CC	denotes	and
T7955	6729-6733	NNS	denotes	data
T7957	6734-6737	RB	denotes	not
T7956	6738-6743	VBN	denotes	shown
T7958	6743-6744	-RRB-	denotes	)
T7959	6744-6745	.	denotes	.
T7960	6745-6849	sentence	denotes	Our data showed that mr-s is predominantly expressed in developing photoreceptors and the pineal gland.
T7961	6746-6749	PRP$	denotes	Our
T7962	6750-6754	NNS	denotes	data
T7963	6755-6761	VBD	denotes	showed
T7964	6762-6766	IN	denotes	that
T7966	6767-6769	NN	denotes	mr
T7968	6769-6770	HYPH	denotes	-
T7967	6770-6771	NN	denotes	s
T7969	6772-6774	VBZ	denotes	is
T7970	6775-6788	RB	denotes	predominantly
T7965	6789-6798	VBN	denotes	expressed
T7971	6799-6801	IN	denotes	in
T7972	6802-6812	VBG	denotes	developing
T7973	6813-6827	NNS	denotes	photoreceptors
T7974	6828-6831	CC	denotes	and
T7975	6832-6835	DT	denotes	the
T7977	6836-6842	JJ	denotes	pineal
T7976	6843-6848	NN	denotes	gland
T7978	6848-6849	.	denotes	.
T9715	6851-6861	NN	denotes	Regulation
T9716	6862-6864	IN	denotes	of
T9717	6865-6867	NN	denotes	mr
T9719	6867-6868	HYPH	denotes	-
T9718	6868-6869	NN	denotes	s
T9720	6870-6883	NN	denotes	transcription
T9721	6884-6886	IN	denotes	by
T9722	6887-6890	NN	denotes	Crx
T9724	6891-6902	NN	denotes	homeodomain
T9723	6903-6910	NN	denotes	protein
T9725	6910-7022	sentence	denotes	Transcription factors Otx2 and Crx are known to be key regulators of retinal photoreceptor development [3,8,9].
T9726	6911-6924	NN	denotes	Transcription
T9727	6925-6932	NNS	denotes	factors
T9729	6933-6937	NN	denotes	Otx2
T9730	6938-6941	CC	denotes	and
T9731	6942-6945	NN	denotes	Crx
T9732	6946-6949	VBP	denotes	are
T9728	6950-6955	VBN	denotes	known
T9733	6956-6958	TO	denotes	to
T9734	6959-6961	VB	denotes	be
T9735	6962-6965	JJ	denotes	key
T9736	6966-6976	NNS	denotes	regulators
T9737	6977-6979	IN	denotes	of
T9738	6980-6987	JJ	denotes	retinal
T9740	6988-7001	NN	denotes	photoreceptor
T9739	7002-7013	NN	denotes	development
T9741	7014-7015	-LRB-	denotes	[
T9743	7015-7016	CD	denotes	3
T9744	7016-7017	,	denotes	,
T9745	7017-7018	CD	denotes	8
T9746	7018-7019	,	denotes	,
T9742	7019-7020	CD	denotes	9
T9747	7020-7021	-RRB-	denotes	]
T9748	7021-7022	.	denotes	.
T9749	7022-7230	sentence	denotes	Although both Otx2 and Crx are expressed in developing photoreceptor cells in the embryonic retina, only Crx is highly expressed in the postnatal photoreceptors, suggesting that mr-s may be regulated by Crx.
T9750	7023-7031	IN	denotes	Although
T9752	7032-7036	CC	denotes	both
T9753	7037-7041	NN	denotes	Otx2
T9754	7042-7045	CC	denotes	and
T9755	7046-7049	NN	denotes	Crx
T9756	7050-7053	VBP	denotes	are
T9751	7054-7063	VBN	denotes	expressed
T9758	7064-7066	IN	denotes	in
T9759	7067-7077	VBG	denotes	developing
T9761	7078-7091	NN	denotes	photoreceptor
T9760	7092-7097	NNS	denotes	cells
T9762	7098-7100	IN	denotes	in
T9763	7101-7104	DT	denotes	the
T9765	7105-7114	JJ	denotes	embryonic
T9764	7115-7121	NN	denotes	retina
T9766	7121-7123	,	denotes	,
T9767	7123-7127	RB	denotes	only
T9768	7128-7131	NN	denotes	Crx
T9769	7132-7134	VBZ	denotes	is
T9770	7135-7141	RB	denotes	highly
T9757	7142-7151	VBN	denotes	expressed
T9771	7152-7154	IN	denotes	in
T9772	7155-7158	DT	denotes	the
T9774	7159-7168	JJ	denotes	postnatal
T9773	7169-7183	NNS	denotes	photoreceptors
T9775	7183-7185	,	denotes	,
T9776	7185-7195	VBG	denotes	suggesting
T9777	7196-7200	IN	denotes	that
T9779	7201-7203	NN	denotes	mr
T9781	7203-7204	HYPH	denotes	-
T9780	7204-7205	NN	denotes	s
T9782	7206-7209	MD	denotes	may
T9783	7210-7212	VB	denotes	be
T9778	7213-7222	VBN	denotes	regulated
T9784	7223-7225	IN	denotes	by
T9785	7226-7229	NN	denotes	Crx
T9786	7229-7230	.	denotes	.
T9787	7230-7377	sentence	denotes	To test whether Crx regulates mr-s transcription, we performed in situ hybridization of mr-s mRNA on wild-type and Crx KO P5 retinas (Fig. 3A, B).
T9788	7231-7233	TO	denotes	To
T9789	7234-7238	VB	denotes	test
T9791	7239-7246	IN	denotes	whether
T9793	7247-7250	NN	denotes	Crx
T9792	7251-7260	VBZ	denotes	regulates
T9794	7261-7263	NN	denotes	mr
T9796	7263-7264	HYPH	denotes	-
T9795	7264-7265	NN	denotes	s
T9797	7266-7279	NN	denotes	transcription
T9798	7279-7281	,	denotes	,
T9799	7281-7283	PRP	denotes	we
T9790	7284-7293	VBD	denotes	performed
T9800	7294-7296	FW	denotes	in
T9801	7297-7301	FW	denotes	situ
T9802	7302-7315	NN	denotes	hybridization
T9803	7316-7318	IN	denotes	of
T9804	7319-7321	NN	denotes	mr
T9806	7321-7322	HYPH	denotes	-
T9805	7322-7323	NN	denotes	s
T9807	7324-7328	NN	denotes	mRNA
T9808	7329-7331	IN	denotes	on
T9809	7332-7336	JJ	denotes	wild
T9811	7336-7337	HYPH	denotes	-
T9810	7337-7341	NN	denotes	type
T9813	7342-7345	CC	denotes	and
T9814	7346-7349	NN	denotes	Crx
T9815	7350-7352	NN	denotes	KO
T9816	7353-7355	NN	denotes	P5
T9812	7356-7363	NNS	denotes	retinas
T9817	7364-7365	-LRB-	denotes	(
T9819	7365-7369	NN	denotes	Fig.
T9820	7370-7372	NN	denotes	3A
T9821	7372-7374	,	denotes	,
T9818	7374-7375	NN	denotes	B
T9822	7375-7376	-RRB-	denotes	)
T9823	7376-7377	.	denotes	.
T9824	7377-7455	sentence	denotes	In the Crx KO retina, the mr-s transcript was dramatically reduced (Fig. 3B).
T9825	7378-7380	IN	denotes	In
T9827	7381-7384	DT	denotes	the
T9829	7385-7388	NN	denotes	Crx
T9830	7389-7391	NN	denotes	KO
T9828	7392-7398	NN	denotes	retina
T9831	7398-7400	,	denotes	,
T9832	7400-7403	DT	denotes	the
T9834	7404-7406	NN	denotes	mr
T9836	7406-7407	HYPH	denotes	-
T9835	7407-7408	NN	denotes	s
T9833	7409-7419	NN	denotes	transcript
T9837	7420-7423	VBD	denotes	was
T9838	7424-7436	RB	denotes	dramatically
T9826	7437-7444	VBN	denotes	reduced
T9839	7445-7446	-LRB-	denotes	(
T9841	7446-7450	NN	denotes	Fig.
T9840	7451-7453	NN	denotes	3B
T9842	7453-7454	-RRB-	denotes	)
T9843	7454-7455	.	denotes	.
T9844	7455-7521	sentence	denotes	This indicates that Crx is essential for transactivation of mr-s.
T9845	7456-7460	DT	denotes	This
T9846	7461-7470	VBZ	denotes	indicates
T9847	7471-7475	IN	denotes	that
T9849	7476-7479	NN	denotes	Crx
T9848	7480-7482	VBZ	denotes	is
T9850	7483-7492	JJ	denotes	essential
T9851	7493-7496	IN	denotes	for
T9852	7497-7512	NN	denotes	transactivation
T9853	7513-7515	IN	denotes	of
T9854	7516-7518	NN	denotes	mr
T9856	7518-7519	HYPH	denotes	-
T9855	7519-7520	NN	denotes	s
T9857	7520-7521	.	denotes	.
T9858	7521-7738	sentence	denotes	Moreover, to test whether the transcription of mr-s is also regulated by Crx in the pineal gland, RT-PCR analysis was used to compare transcriptional levels of mr-s in P28 wild-type and Crx KO pineal gland (Fig. 3C).
T9859	7522-7530	RB	denotes	Moreover
T9861	7530-7532	,	denotes	,
T9862	7532-7534	TO	denotes	to
T9863	7535-7539	VB	denotes	test
T9864	7540-7547	IN	denotes	whether
T9866	7548-7551	DT	denotes	the
T9867	7552-7565	NN	denotes	transcription
T9868	7566-7568	IN	denotes	of
T9869	7569-7571	NN	denotes	mr
T9871	7571-7572	HYPH	denotes	-
T9870	7572-7573	NN	denotes	s
T9872	7574-7576	VBZ	denotes	is
T9873	7577-7581	RB	denotes	also
T9865	7582-7591	VBN	denotes	regulated
T9874	7592-7594	IN	denotes	by
T9875	7595-7598	NN	denotes	Crx
T9876	7599-7601	IN	denotes	in
T9877	7602-7605	DT	denotes	the
T9879	7606-7612	JJ	denotes	pineal
T9878	7613-7618	NN	denotes	gland
T9880	7618-7620	,	denotes	,
T9881	7620-7622	NN	denotes	RT
T9883	7622-7623	HYPH	denotes	-
T9882	7623-7626	NN	denotes	PCR
T9884	7627-7635	NN	denotes	analysis
T9885	7636-7639	VBD	denotes	was
T9860	7640-7644	VBN	denotes	used
T9886	7645-7647	TO	denotes	to
T9887	7648-7655	VB	denotes	compare
T9888	7656-7671	JJ	denotes	transcriptional
T9889	7672-7678	NNS	denotes	levels
T9890	7679-7681	IN	denotes	of
T9891	7682-7684	NN	denotes	mr
T9893	7684-7685	HYPH	denotes	-
T9892	7685-7686	NN	denotes	s
T9894	7687-7689	IN	denotes	in
T9895	7690-7693	NN	denotes	P28
T9897	7694-7698	JJ	denotes	wild
T9899	7698-7699	HYPH	denotes	-
T9898	7699-7703	NN	denotes	type
T9900	7704-7707	CC	denotes	and
T9901	7708-7711	NN	denotes	Crx
T9902	7712-7714	NN	denotes	KO
T9903	7715-7721	JJ	denotes	pineal
T9896	7722-7727	NN	denotes	gland
T9904	7728-7729	-LRB-	denotes	(
T9906	7729-7733	NN	denotes	Fig.
T9905	7734-7736	NN	denotes	3C
T9907	7736-7737	-RRB-	denotes	)
T9908	7737-7738	.	denotes	.
T9909	7738-7843	sentence	denotes	The results revealed that mr-s transcription was significantly downregulated in the Crx KO pineal gland.
T9910	7739-7742	DT	denotes	The
T9911	7743-7750	NNS	denotes	results
T9912	7751-7759	VBD	denotes	revealed
T9913	7760-7764	IN	denotes	that
T9915	7765-7767	NN	denotes	mr
T9917	7767-7768	HYPH	denotes	-
T9916	7768-7769	NN	denotes	s
T9918	7770-7783	NN	denotes	transcription
T9919	7784-7787	VBD	denotes	was
T9920	7788-7801	RB	denotes	significantly
T9914	7802-7815	VBN	denotes	downregulated
T9921	7816-7818	IN	denotes	in
T9922	7819-7822	DT	denotes	the
T9924	7823-7826	NN	denotes	Crx
T9925	7827-7829	NN	denotes	KO
T9926	7830-7836	JJ	denotes	pineal
T9923	7837-7842	NN	denotes	gland
T9927	7842-7843	.	denotes	.
T9928	7843-7974	sentence	denotes	Taken together, these data indicate that mr-s transcription is regulated by Crx in the developing photoreceptors and pineal gland.
T9929	7844-7849	VBN	denotes	Taken
T9931	7850-7858	RB	denotes	together
T9932	7858-7860	,	denotes	,
T9933	7860-7865	DT	denotes	these
T9934	7866-7870	NNS	denotes	data
T9930	7871-7879	VBP	denotes	indicate
T9935	7880-7884	IN	denotes	that
T9937	7885-7887	NN	denotes	mr
T9939	7887-7888	HYPH	denotes	-
T9938	7888-7889	NN	denotes	s
T9940	7890-7903	NN	denotes	transcription
T9941	7904-7906	VBZ	denotes	is
T9936	7907-7916	VBN	denotes	regulated
T9942	7917-7919	IN	denotes	by
T9943	7920-7923	NN	denotes	Crx
T9944	7924-7926	IN	denotes	in
T9945	7927-7930	DT	denotes	the
T9947	7931-7941	VBG	denotes	developing
T9946	7942-7956	NNS	denotes	photoreceptors
T9948	7957-7960	CC	denotes	and
T9949	7961-7967	JJ	denotes	pineal
T9950	7968-7973	NN	denotes	gland
T9951	7973-7974	.	denotes	.
T9952	7974-7975	sentence	denotes
T30251	7985-7988	DT	denotes	The
T30252	7989-8002	NN	denotes	transcription
T30254	8003-8005	IN	denotes	of
T30255	8006-8008	NN	denotes	mr
T30257	8008-8009	HYPH	denotes	-
T30256	8009-8010	NN	denotes	s
T30258	8011-8013	VBZ	denotes	is
T30253	8014-8023	VBN	denotes	regulated
T30259	8024-8026	IN	denotes	by
T30260	8027-8030	NN	denotes	Crx
T30261	8030-8031	.	denotes	.
T30262	8031-8154	sentence	denotes	(A, B) In situ hybridization using a probe for mouse mr-s was performed on the wild-type (A) and Crx KO retinas (B) at P5.
T30263	8032-8033	-LRB-	denotes	(
T30264	8033-8034	LS	denotes	A
T30266	8034-8036	,	denotes	,
T30267	8036-8037	LS	denotes	B
T30268	8037-8038	-RRB-	denotes	)
T30269	8039-8041	FW	denotes	In
T30270	8042-8046	FW	denotes	situ
T30271	8047-8060	NN	denotes	hybridization
T30272	8061-8066	VBG	denotes	using
T30273	8067-8068	DT	denotes	a
T30274	8069-8074	NN	denotes	probe
T30275	8075-8078	IN	denotes	for
T30276	8079-8084	NN	denotes	mouse
T30278	8085-8087	NN	denotes	mr
T30279	8087-8088	HYPH	denotes	-
T30277	8088-8089	NN	denotes	s
T30280	8090-8093	VBD	denotes	was
T30265	8094-8103	VBN	denotes	performed
T30281	8104-8106	IN	denotes	on
T30282	8107-8110	DT	denotes	the
T30284	8111-8115	JJ	denotes	wild
T30286	8115-8116	HYPH	denotes	-
T30285	8116-8120	NN	denotes	type
T30287	8121-8122	-LRB-	denotes	(
T30288	8122-8123	NN	denotes	A
T30289	8123-8124	-RRB-	denotes	)
T30290	8125-8128	CC	denotes	and
T30291	8129-8132	NN	denotes	Crx
T30292	8133-8135	NN	denotes	KO
T30283	8136-8143	NNS	denotes	retinas
T30293	8144-8145	-LRB-	denotes	(
T30294	8145-8146	NN	denotes	B
T30295	8146-8147	-RRB-	denotes	)
T30296	8148-8150	IN	denotes	at
T30297	8151-8153	NN	denotes	P5
T30298	8153-8154	.	denotes	.
T30299	8154-8220	sentence	denotes	mr-s expression was drastically reduced in the Crx KO retina (B).
T30300	8155-8157	NN	denotes	mr
T30302	8157-8158	HYPH	denotes	-
T30301	8158-8159	NN	denotes	s
T30303	8160-8170	NN	denotes	expression
T30305	8171-8174	VBD	denotes	was
T30306	8175-8186	RB	denotes	drastically
T30304	8187-8194	VBN	denotes	reduced
T30307	8195-8197	IN	denotes	in
T30308	8198-8201	DT	denotes	the
T30310	8202-8205	NN	denotes	Crx
T30311	8206-8208	NN	denotes	KO
T30309	8209-8215	NN	denotes	retina
T30312	8216-8217	-LRB-	denotes	(
T30313	8217-8218	NN	denotes	B
T30314	8218-8219	-RRB-	denotes	)
T30315	8219-8220	.	denotes	.
T30316	8220-8239	sentence	denotes	Scale bar, 100 μm.
T30317	8221-8226	NN	denotes	Scale
T30318	8227-8230	NN	denotes	bar
T30319	8230-8232	,	denotes	,
T30320	8232-8235	CD	denotes	100
T30321	8236-8238	NN	denotes	μm
T30322	8238-8239	.	denotes	.
T30323	8239-8340	sentence	denotes	(C) RT-PCR analysis of total RNAs extracted from the pineal glands of P5 wild-type and Crx KO mouse.
T30324	8240-8241	-LRB-	denotes	(
T30325	8241-8242	LS	denotes	C
T30327	8242-8243	-RRB-	denotes	)
T30328	8244-8246	NN	denotes	RT
T30330	8246-8247	HYPH	denotes	-
T30329	8247-8250	NN	denotes	PCR
T30326	8251-8259	NN	denotes	analysis
T30331	8260-8262	IN	denotes	of
T30332	8263-8268	JJ	denotes	total
T30333	8269-8273	NNS	denotes	RNAs
T30334	8274-8283	VBN	denotes	extracted
T30335	8284-8288	IN	denotes	from
T30336	8289-8292	DT	denotes	the
T30338	8293-8299	JJ	denotes	pineal
T30337	8300-8306	NNS	denotes	glands
T30339	8307-8309	IN	denotes	of
T30340	8310-8312	NN	denotes	P5
T30342	8313-8317	JJ	denotes	wild
T30344	8317-8318	HYPH	denotes	-
T30343	8318-8322	NN	denotes	type
T30345	8323-8326	CC	denotes	and
T30346	8327-8330	NN	denotes	Crx
T30347	8331-8333	NN	denotes	KO
T30341	8334-8339	NN	denotes	mouse
T30348	8339-8340	.	denotes	.
T30349	8340-8463	sentence	denotes	The upper and lower lanes show PCR products amplified by the primer pairs specific for mr-s and G3PDH cDNAs, respectively.
T30350	8341-8344	DT	denotes	The
T30352	8345-8350	JJ	denotes	upper
T30353	8351-8354	CC	denotes	and
T30354	8355-8360	JJ	denotes	lower
T30351	8361-8366	NNS	denotes	lanes
T30355	8367-8371	VBP	denotes	show
T30356	8372-8375	NN	denotes	PCR
T30357	8376-8384	NNS	denotes	products
T30358	8385-8394	VBN	denotes	amplified
T30359	8395-8397	IN	denotes	by
T30360	8398-8401	DT	denotes	the
T30362	8402-8408	NN	denotes	primer
T30361	8409-8414	NNS	denotes	pairs
T30363	8415-8423	JJ	denotes	specific
T30364	8424-8427	IN	denotes	for
T30365	8428-8430	NN	denotes	mr
T30367	8430-8431	HYPH	denotes	-
T30366	8431-8432	NN	denotes	s
T30368	8433-8436	CC	denotes	and
T30369	8437-8442	NN	denotes	G3PDH
T30370	8443-8448	NNS	denotes	cDNAs
T30371	8448-8450	,	denotes	,
T30372	8450-8462	RB	denotes	respectively
T30373	8462-8463	.	denotes	.
T30374	8463-8507	sentence	denotes	Water was used for control RT-PCR reaction.
T30375	8464-8469	NN	denotes	Water
T30377	8470-8473	VBD	denotes	was
T30376	8474-8478	VBN	denotes	used
T30378	8479-8482	IN	denotes	for
T30379	8483-8490	NN	denotes	control
T30381	8491-8493	NN	denotes	RT
T30383	8493-8494	HYPH	denotes	-
T30382	8494-8497	NN	denotes	PCR
T30380	8498-8506	NN	denotes	reaction
T30384	8506-8507	.	denotes	.
T30385	8507-8552	sentence	denotes	(D-F) Crx transactivates mr-s transcription.
T30386	8508-8509	-LRB-	denotes	(
T30387	8509-8510	LS	denotes	D
T30389	8510-8511	SYM	denotes	-
T30390	8511-8512	LS	denotes	F
T30391	8512-8513	-RRB-	denotes	)
T30392	8514-8517	NN	denotes	Crx
T30388	8518-8532	VBZ	denotes	transactivates
T30393	8533-8535	NN	denotes	mr
T30395	8535-8536	HYPH	denotes	-
T30394	8536-8537	NN	denotes	s
T30396	8538-8551	NN	denotes	transcription
T30397	8551-8552	.	denotes	.
T30398	8552-8606	sentence	denotes	Reporter plasmids for the luciferase assay are shown.
T30399	8553-8561	NN	denotes	Reporter
T30400	8562-8570	NNS	denotes	plasmids
T30402	8571-8574	IN	denotes	for
T30403	8575-8578	DT	denotes	the
T30405	8579-8589	NN	denotes	luciferase
T30404	8590-8595	NN	denotes	assay
T30406	8596-8599	VBP	denotes	are
T30401	8600-8605	VBN	denotes	shown
T30407	8605-8606	.	denotes	.
T30408	8606-8650	sentence	denotes	Blue boxes represent Crx binding sites (D).
T30409	8607-8611	JJ	denotes	Blue
T30410	8612-8617	NNS	denotes	boxes
T30411	8618-8627	VBP	denotes	represent
T30412	8628-8631	NN	denotes	Crx
T30414	8632-8639	NN	denotes	binding
T30413	8640-8645	NNS	denotes	sites
T30415	8646-8647	-LRB-	denotes	(
T30416	8647-8648	NN	denotes	D
T30417	8648-8649	-RRB-	denotes	)
T30418	8649-8650	.	denotes	.
T30419	8650-8783	sentence	denotes	Relative activity of the luciferase is indicated when Pro1.2k was co-transfected with Crx, Otx2, Nrl, and Crx+Nrl, respectively (E).
T30420	8651-8659	JJ	denotes	Relative
T30421	8660-8668	NN	denotes	activity
T30423	8669-8671	IN	denotes	of
T30424	8672-8675	DT	denotes	the
T30425	8676-8686	NN	denotes	luciferase
T30426	8687-8689	VBZ	denotes	is
T30422	8690-8699	VBN	denotes	indicated
T30427	8700-8704	WRB	denotes	when
T30429	8705-8712	NN	denotes	Pro1.2k
T30430	8713-8716	VBD	denotes	was
T30428	8717-8731	VBN	denotes	co-transfected
T30431	8732-8736	IN	denotes	with
T30432	8737-8740	NN	denotes	Crx
T30433	8740-8742	,	denotes	,
T30434	8742-8746	NN	denotes	Otx2
T30435	8746-8748	,	denotes	,
T30436	8748-8751	NN	denotes	Nrl
T30437	8751-8753	,	denotes	,
T30438	8753-8756	CC	denotes	and
T30439	8757-8760	NN	denotes	Crx
T30441	8760-8761	SYM	denotes	+
T30440	8761-8764	NN	denotes	Nrl
T30442	8764-8766	,	denotes	,
T30443	8766-8778	RB	denotes	respectively
T30444	8779-8780	-LRB-	denotes	(
T30445	8780-8781	NN	denotes	E
T30446	8781-8782	-RRB-	denotes	)
T30447	8782-8783	.	denotes	.
T30448	8783-8970	sentence	denotes	Fold activation is indicated when Pro1.2k, mut1259, mut198, mut72 and mut all were co-transfected with the Crx expression vector (Crx+) or the empty vector (Crx-) into HEK293T cells (F).
T30449	8784-8788	NN	denotes	Fold
T30450	8789-8799	NN	denotes	activation
T30452	8800-8802	VBZ	denotes	is
T30451	8803-8812	VBN	denotes	indicated
T30453	8813-8817	WRB	denotes	when
T30455	8818-8825	NN	denotes	Pro1.2k
T30456	8825-8827	,	denotes	,
T30457	8827-8834	NN	denotes	mut1259
T30458	8834-8836	,	denotes	,
T30459	8836-8842	NN	denotes	mut198
T30460	8842-8844	,	denotes	,
T30461	8844-8849	NN	denotes	mut72
T30462	8850-8853	CC	denotes	and
T30463	8854-8857	NN	denotes	mut
T30464	8858-8861	NN	denotes	all
T30465	8862-8866	VBD	denotes	were
T30454	8867-8881	VBN	denotes	co-transfected
T30466	8882-8886	IN	denotes	with
T30467	8887-8890	DT	denotes	the
T30469	8891-8894	NN	denotes	Crx
T30470	8895-8905	NN	denotes	expression
T30468	8906-8912	NN	denotes	vector
T30471	8913-8914	-LRB-	denotes	(
T30472	8914-8917	NN	denotes	Crx
T30473	8917-8918	SYM	denotes	+
T30474	8918-8919	-RRB-	denotes	)
T30475	8920-8922	CC	denotes	or
T30476	8923-8926	DT	denotes	the
T30478	8927-8932	JJ	denotes	empty
T30477	8933-8939	NN	denotes	vector
T30479	8940-8941	-LRB-	denotes	(
T30480	8941-8944	NN	denotes	Crx
T30481	8944-8945	SYM	denotes	-
T30482	8945-8946	-RRB-	denotes	)
T30483	8947-8951	IN	denotes	into
T30484	8952-8959	NN	denotes	HEK293T
T30485	8960-8965	NNS	denotes	cells
T30486	8966-8967	-LRB-	denotes	(
T30487	8967-8968	NN	denotes	F
T30488	8968-8969	-RRB-	denotes	)
T30489	8969-8970	.	denotes	.
T30490	8970-9015	sentence	denotes	Error bars represent standard error of mean.
T30491	8971-8976	NN	denotes	Error
T30492	8977-8981	NNS	denotes	bars
T30493	8982-8991	VBP	denotes	represent
T30494	8992-9000	JJ	denotes	standard
T30495	9001-9006	NN	denotes	error
T30496	9007-9009	IN	denotes	of
T30497	9010-9014	NN	denotes	mean
T30498	9014-9015	.	denotes	.
T9954	9016-9017	DT	denotes	T
T9953	9016-9022	sentence	denotes	To fur
T9955	9017-9018	NN	denotes	o
T9957	9019-9020	IN	denotes	f
T9956	9020-9021	VBN	denotes	u
T9958	9021-9022	NN	denotes	r
T9960	9022-9023	FW	denotes	t
T9959	9022-9030	sentence	denotes	ther exa
T9961	9023-9024	NN	denotes	h
T9962	9024-9025	NN	denotes	e
T9963	9025-9026	IN	denotes	r
T9964	9027-9028	NN	denotes	e
T9966	9028-9029	NN	denotes	x
T9965	9029-9030	NNS	denotes	a
T9968	9030-9031	NN	denotes	m
T9967	9030-9035	sentence	denotes	mine
T9969	9031-9033	NN	denotes	in
T9970	9033-9034	VBN	denotes	e
T9971	9035-9036	sentence	denotes	w
T9972	9035-9036	JJ	denotes	w
T9974	9036-9038	DT	denotes	he
T9973	9036-9043	sentence	denotes	hether
T9975	9038-9039	NNS	denotes	t
T9976	9039-9041	DT	denotes	he
T9977	9041-9042	NN	denotes	r
T9980	9043-9044	NN	denotes	C
T9978	9043-9045	sentence	denotes	Cr
T9979	9044-9045	NN	denotes	r
T9983	9045-9046	NN	denotes	x
T9981	9045-9049	sentence	denotes	x re
T9982	9047-9049	VBZ	denotes	re
T9984	9049-9050	sentence	denotes	g
T9985	9049-9050	NN	denotes	g
T9987	9050-9051	NN	denotes	u
T9986	9050-9055	sentence	denotes	ulate
T9988	9051-9052	NN	denotes	l
T9989	9052-9053	CC	denotes	a
T9990	9053-9055	RB	denotes	te
T9992	9055-9056	VBZ	denotes	s
T9991	9055-9064	sentence	denotes	s mr-s tr
T9994	9057-9058	NN	denotes	m
T9995	9058-9059	VBD	denotes	r
T9993	9059-9061	VBN	denotes	-s
T9996	9062-9063	IN	denotes	t
T9997	9063-9064	NN	denotes	r
T9999	9064-9065	NNS	denotes	a
T9998	9064-9067	sentence	denotes	ans
T10000	9065-9066	VBP	denotes	n
T10001	9066-9067	JJ	denotes	s
T10004	9067-9075	NN	denotes	cription
T10002	9067-9307	sentence	denotes	cription directly or not, we next performed a luciferase assay using the 1.2-kb proximal promoter region of mr-s fused to a luciferase gene as a luciferase reporter (Fig. 3D, Pro1.2k) and the Crx, Otx2, Nrl expression vectors, respectively.
T10005	9076-9084	RB	denotes	directly
T10006	9085-9087	CC	denotes	or
T10007	9088-9091	RB	denotes	not
T10008	9091-9093	,	denotes	,
T10009	9093-9095	PRP	denotes	we
T10010	9096-9100	RB	denotes	next
T10003	9101-9110	VBD	denotes	performed
T10011	9111-9112	DT	denotes	a
T10013	9113-9123	NN	denotes	luciferase
T10012	9124-9129	NN	denotes	assay
T10014	9130-9135	VBG	denotes	using
T10015	9136-9139	DT	denotes	the
T10017	9140-9143	CD	denotes	1.2
T10019	9143-9144	HYPH	denotes	-
T10018	9144-9146	NN	denotes	kb
T10020	9147-9155	JJ	denotes	proximal
T10021	9156-9164	NN	denotes	promoter
T10016	9165-9171	NN	denotes	region
T10022	9172-9174	IN	denotes	of
T10023	9175-9177	NN	denotes	mr
T10025	9177-9178	HYPH	denotes	-
T10024	9178-9179	NN	denotes	s
T10026	9180-9185	VBN	denotes	fused
T10027	9186-9188	IN	denotes	to
T10028	9189-9190	DT	denotes	a
T10030	9191-9201	NN	denotes	luciferase
T10029	9202-9206	NN	denotes	gene
T10031	9207-9209	IN	denotes	as
T10032	9210-9211	DT	denotes	a
T10034	9212-9222	NN	denotes	luciferase
T10033	9223-9231	NN	denotes	reporter
T10035	9232-9233	-LRB-	denotes	(
T10037	9233-9237	NN	denotes	Fig.
T10036	9238-9240	NN	denotes	3D
T10038	9240-9242	,	denotes	,
T10039	9242-9249	NN	denotes	Pro1.2k
T10040	9249-9250	-RRB-	denotes	)
T10041	9251-9254	CC	denotes	and
T10042	9255-9258	DT	denotes	the
T10044	9259-9262	NN	denotes	Crx
T10046	9262-9264	,	denotes	,
T10047	9264-9268	NN	denotes	Otx2
T10048	9268-9270	,	denotes	,
T10045	9270-9273	NN	denotes	Nrl
T10049	9274-9284	NN	denotes	expression
T10043	9285-9292	NNS	denotes	vectors
T10050	9292-9294	,	denotes	,
T10051	9294-9306	RB	denotes	respectively
T10052	9306-9307	.	denotes	.
T10053	9307-9404	sentence	denotes	This 1.2-kb region of the mr-s upstream sequence contains three Crx binding consensus sequences.
T10054	9308-9312	DT	denotes	This
T10056	9313-9316	CD	denotes	1.2
T10058	9316-9317	HYPH	denotes	-
T10057	9317-9319	NN	denotes	kb
T10055	9320-9326	NN	denotes	region
T10060	9327-9329	IN	denotes	of
T10061	9330-9333	DT	denotes	the
T10063	9334-9336	NN	denotes	mr
T10065	9336-9337	HYPH	denotes	-
T10064	9337-9338	NN	denotes	s
T10066	9339-9347	JJ	denotes	upstream
T10062	9348-9356	NN	denotes	sequence
T10059	9357-9365	VBZ	denotes	contains
T10067	9366-9371	CD	denotes	three
T10069	9372-9375	NN	denotes	Crx
T10070	9376-9383	NN	denotes	binding
T10071	9384-9393	NN	denotes	consensus
T10068	9394-9403	NNS	denotes	sequences
T10072	9403-9404	.	denotes	.
T10073	9404-9665	sentence	denotes	As shown in Fig. 3E, the luciferase activity was significantly upregulated when the Crx or Otx2 expression vector was co-introduced with Pro1.2k into HEK293T cells, while the luciferase activity was not altered when the Nrl expression vector was co-introduced.
T10074	9405-9407	IN	denotes	As
T10075	9408-9413	VBN	denotes	shown
T10077	9414-9416	IN	denotes	in
T10078	9417-9421	NN	denotes	Fig.
T10079	9422-9424	NN	denotes	3E
T10080	9424-9426	,	denotes	,
T10081	9426-9429	DT	denotes	the
T10083	9430-9440	NN	denotes	luciferase
T10082	9441-9449	NN	denotes	activity
T10084	9450-9453	VBD	denotes	was
T10085	9454-9467	RB	denotes	significantly
T10076	9468-9479	VBN	denotes	upregulated
T10086	9480-9484	WRB	denotes	when
T10088	9485-9488	DT	denotes	the
T10090	9489-9492	NN	denotes	Crx
T10091	9493-9495	CC	denotes	or
T10092	9496-9500	NN	denotes	Otx2
T10093	9501-9511	NN	denotes	expression
T10089	9512-9518	NN	denotes	vector
T10094	9519-9522	VBD	denotes	was
T10087	9523-9536	VBN	denotes	co-introduced
T10095	9537-9541	IN	denotes	with
T10096	9542-9549	NN	denotes	Pro1.2k
T10097	9550-9554	IN	denotes	into
T10098	9555-9562	NN	denotes	HEK293T
T10099	9563-9568	NNS	denotes	cells
T10100	9568-9570	,	denotes	,
T10101	9570-9575	IN	denotes	while
T10103	9576-9579	DT	denotes	the
T10105	9580-9590	NN	denotes	luciferase
T10104	9591-9599	NN	denotes	activity
T10106	9600-9603	VBD	denotes	was
T10107	9604-9607	RB	denotes	not
T10102	9608-9615	VBN	denotes	altered
T10108	9616-9620	WRB	denotes	when
T10110	9621-9624	DT	denotes	the
T10112	9625-9628	NN	denotes	Nrl
T10113	9629-9639	NN	denotes	expression
T10111	9640-9646	NN	denotes	vector
T10114	9647-9650	VBD	denotes	was
T10109	9651-9664	VBN	denotes	co-introduced
T10115	9664-9665	.	denotes	.
T10116	9665-9817	sentence	denotes	A previous report suggested that the transcriptional activity of Crx is augmented with Nrl when the rhodopsin promoter was used as a reporter gene [6].
T10117	9666-9667	DT	denotes	A
T10119	9668-9676	JJ	denotes	previous
T10118	9677-9683	NN	denotes	report
T10120	9684-9693	VBD	denotes	suggested
T10121	9694-9698	IN	denotes	that
T10123	9699-9702	DT	denotes	the
T10125	9703-9718	JJ	denotes	transcriptional
T10124	9719-9727	NN	denotes	activity
T10126	9728-9730	IN	denotes	of
T10127	9731-9734	NN	denotes	Crx
T10128	9735-9737	VBZ	denotes	is
T10122	9738-9747	VBN	denotes	augmented
T10129	9748-9752	IN	denotes	with
T10130	9753-9756	NN	denotes	Nrl
T10131	9757-9761	WRB	denotes	when
T10133	9762-9765	DT	denotes	the
T10135	9766-9775	NN	denotes	rhodopsin
T10134	9776-9784	NN	denotes	promoter
T10136	9785-9788	VBD	denotes	was
T10132	9789-9793	VBN	denotes	used
T10137	9794-9796	IN	denotes	as
T10138	9797-9798	DT	denotes	a
T10140	9799-9807	NN	denotes	reporter
T10139	9808-9812	NN	denotes	gene
T10141	9813-9814	-LRB-	denotes	[
T10142	9814-9815	CD	denotes	6
T10143	9815-9816	-RRB-	denotes	]
T10144	9816-9817	.	denotes	.
T10145	9817-10066	sentence	denotes	On the other hand, our present data showed that the luciferase gene expression was not upregulated when both Crx and Nrl expression vectors were co-introduced with Pro1.2k compared to the activity when the Crx only expression vector was introduced.
T10146	9818-9820	IN	denotes	On
T10148	9821-9824	DT	denotes	the
T10150	9825-9830	JJ	denotes	other
T10149	9831-9835	NN	denotes	hand
T10151	9835-9837	,	denotes	,
T10152	9837-9840	PRP$	denotes	our
T10154	9841-9848	JJ	denotes	present
T10153	9849-9853	NNS	denotes	data
T10147	9854-9860	VBD	denotes	showed
T10155	9861-9865	IN	denotes	that
T10157	9866-9869	DT	denotes	the
T10159	9870-9880	NN	denotes	luciferase
T10160	9881-9885	NN	denotes	gene
T10158	9886-9896	NN	denotes	expression
T10161	9897-9900	VBD	denotes	was
T10162	9901-9904	RB	denotes	not
T10156	9905-9916	VBN	denotes	upregulated
T10163	9917-9921	WRB	denotes	when
T10165	9922-9926	CC	denotes	both
T10166	9927-9930	NN	denotes	Crx
T10168	9931-9934	CC	denotes	and
T10169	9935-9938	NN	denotes	Nrl
T10170	9939-9949	NN	denotes	expression
T10167	9950-9957	NNS	denotes	vectors
T10171	9958-9962	VBD	denotes	were
T10164	9963-9976	VBN	denotes	co-introduced
T10172	9977-9981	IN	denotes	with
T10173	9982-9989	NN	denotes	Pro1.2k
T10174	9990-9998	VBN	denotes	compared
T10175	9999-10001	IN	denotes	to
T10176	10002-10005	DT	denotes	the
T10177	10006-10014	NN	denotes	activity
T10178	10015-10019	WRB	denotes	when
T10180	10020-10023	DT	denotes	the
T10182	10024-10027	NN	denotes	Crx
T10183	10028-10032	JJ	denotes	only
T10184	10033-10043	NN	denotes	expression
T10181	10044-10050	NN	denotes	vector
T10185	10051-10054	VBD	denotes	was
T10179	10055-10065	VBN	denotes	introduced
T10186	10065-10066	.	denotes	.
T10187	10066-10190	sentence	denotes	This may be due to cell type differences because a cell type of retinal/pineal origin was not used in our luciferase assay.
T10188	10067-10071	DT	denotes	This
T10190	10072-10075	MD	denotes	may
T10189	10076-10078	VB	denotes	be
T10191	10079-10082	IN	denotes	due
T10192	10083-10085	IN	denotes	to
T10193	10086-10090	NN	denotes	cell
T10194	10091-10095	NN	denotes	type
T10195	10096-10107	NNS	denotes	differences
T10196	10108-10115	IN	denotes	because
T10198	10116-10117	DT	denotes	a
T10200	10118-10122	NN	denotes	cell
T10199	10123-10127	NN	denotes	type
T10201	10128-10130	IN	denotes	of
T10202	10131-10138	JJ	denotes	retinal
T10204	10138-10139	HYPH	denotes	/
T10203	10139-10145	JJ	denotes	pineal
T10205	10146-10152	NN	denotes	origin
T10206	10153-10156	VBD	denotes	was
T10207	10157-10160	RB	denotes	not
T10197	10161-10165	VBN	denotes	used
T10208	10166-10168	IN	denotes	in
T10209	10169-10172	PRP$	denotes	our
T10211	10173-10183	NN	denotes	luciferase
T10210	10184-10189	NN	denotes	assay
T10212	10189-10190	.	denotes	.
T10213	10190-10336	sentence	denotes	In addition, our present data showed that Otx2, which is reported to have the same binding consensus as Crx, also transactivated mr-s expression.
T10214	10191-10193	IN	denotes	In
T10216	10194-10202	NN	denotes	addition
T10217	10202-10204	,	denotes	,
T10218	10204-10207	PRP$	denotes	our
T10220	10208-10215	JJ	denotes	present
T10219	10216-10220	NNS	denotes	data
T10215	10221-10227	VBD	denotes	showed
T10221	10228-10232	IN	denotes	that
T10223	10233-10237	NN	denotes	Otx2
T10224	10237-10239	,	denotes	,
T10225	10239-10244	WDT	denotes	which
T10227	10245-10247	VBZ	denotes	is
T10226	10248-10256	VBN	denotes	reported
T10228	10257-10259	TO	denotes	to
T10229	10260-10264	VB	denotes	have
T10230	10265-10268	DT	denotes	the
T10232	10269-10273	JJ	denotes	same
T10233	10274-10281	NN	denotes	binding
T10231	10282-10291	NN	denotes	consensus
T10234	10292-10294	IN	denotes	as
T10235	10295-10298	NN	denotes	Crx
T10236	10298-10300	,	denotes	,
T10237	10300-10304	RB	denotes	also
T10222	10305-10319	VBN	denotes	transactivated
T10238	10320-10322	NN	denotes	mr
T10240	10322-10323	HYPH	denotes	-
T10239	10323-10324	NN	denotes	s
T10241	10325-10335	NN	denotes	expression
T10242	10335-10336	.	denotes	.
T10243	10336-10419	sentence	denotes	As shown in Fig. 2A–F, the expression pattern of mr-s correlates with that of Crx.
T10244	10337-10339	IN	denotes	As
T10245	10340-10345	VBN	denotes	shown
T10247	10346-10348	IN	denotes	in
T10248	10349-10353	NN	denotes	Fig.
T10249	10354-10356	NN	denotes	2A
T10250	10356-10357	SYM	denotes	–
T10251	10357-10358	NN	denotes	F
T10252	10358-10360	,	denotes	,
T10253	10360-10363	DT	denotes	the
T10255	10364-10374	NN	denotes	expression
T10254	10375-10382	NN	denotes	pattern
T10256	10383-10385	IN	denotes	of
T10257	10386-10388	NN	denotes	mr
T10259	10388-10389	HYPH	denotes	-
T10258	10389-10390	NN	denotes	s
T10246	10391-10401	VBZ	denotes	correlates
T10260	10402-10406	IN	denotes	with
T10261	10407-10411	DT	denotes	that
T10262	10412-10414	IN	denotes	of
T10263	10415-10418	NN	denotes	Crx
T10264	10418-10419	.	denotes	.
T10265	10419-10524	sentence	denotes	In contrast, the transcripts of Otx2 are mainly detected in the photoreceptor layer at embryonic stages.
T10266	10420-10422	IN	denotes	In
T10268	10423-10431	NN	denotes	contrast
T10269	10431-10433	,	denotes	,
T10270	10433-10436	DT	denotes	the
T10271	10437-10448	NNS	denotes	transcripts
T10272	10449-10451	IN	denotes	of
T10273	10452-10456	NN	denotes	Otx2
T10274	10457-10460	VBP	denotes	are
T10275	10461-10467	RB	denotes	mainly
T10267	10468-10476	VBN	denotes	detected
T10276	10477-10479	IN	denotes	in
T10277	10480-10483	DT	denotes	the
T10279	10484-10497	NN	denotes	photoreceptor
T10278	10498-10503	NN	denotes	layer
T10280	10504-10506	IN	denotes	at
T10281	10507-10516	JJ	denotes	embryonic
T10282	10517-10523	NNS	denotes	stages
T10283	10523-10524	.	denotes	.
T10284	10524-10609	sentence	denotes	Therefore, we concluded that mr-s transcription is directly regulated mainly by Crx.
T10285	10525-10534	RB	denotes	Therefore
T10287	10534-10536	,	denotes	,
T10288	10536-10538	PRP	denotes	we
T10286	10539-10548	VBD	denotes	concluded
T10289	10549-10553	IN	denotes	that
T10291	10554-10556	NN	denotes	mr
T10293	10556-10557	HYPH	denotes	-
T10292	10557-10558	NN	denotes	s
T10294	10559-10572	NN	denotes	transcription
T10295	10573-10575	VBZ	denotes	is
T10296	10576-10584	RB	denotes	directly
T10290	10585-10594	VBN	denotes	regulated
T10297	10595-10601	RB	denotes	mainly
T10298	10602-10604	IN	denotes	by
T10299	10605-10608	NN	denotes	Crx
T10300	10608-10609	.	denotes	.
T10301	10609-10756	sentence	denotes	We also constructed reporter vectors in which mutations were introduced at the three Crx binding sites (Fig. 3D, mut1259, mut198, mut72, mut all).
T10302	10610-10612	PRP	denotes	We
T10304	10613-10617	RB	denotes	also
T10303	10618-10629	VBD	denotes	constructed
T10305	10630-10638	NN	denotes	reporter
T10306	10639-10646	NNS	denotes	vectors
T10307	10647-10649	IN	denotes	in
T10309	10650-10655	WDT	denotes	which
T10310	10656-10665	NNS	denotes	mutations
T10311	10666-10670	VBD	denotes	were
T10308	10671-10681	VBN	denotes	introduced
T10312	10682-10684	IN	denotes	at
T10313	10685-10688	DT	denotes	the
T10315	10689-10694	CD	denotes	three
T10316	10695-10698	NN	denotes	Crx
T10317	10699-10706	NN	denotes	binding
T10314	10707-10712	NNS	denotes	sites
T10318	10713-10714	-LRB-	denotes	(
T10320	10714-10718	NN	denotes	Fig.
T10321	10719-10721	NN	denotes	3D
T10322	10721-10723	,	denotes	,
T10319	10723-10730	NN	denotes	mut1259
T10323	10730-10732	,	denotes	,
T10324	10732-10738	NN	denotes	mut198
T10325	10738-10740	,	denotes	,
T10326	10740-10745	NN	denotes	mut72
T10327	10745-10747	,	denotes	,
T10328	10747-10750	NN	denotes	mut
T10329	10751-10754	NN	denotes	all
T10330	10754-10755	-RRB-	denotes	)
T10331	10755-10756	.	denotes	.
T10332	10756-10870	sentence	denotes	Then the Crx expression vector was co-introduced with mut1259, mut198, mut72 and mut all, respectively (Fig. 3F).
T10333	10757-10761	RB	denotes	Then
T10335	10762-10765	DT	denotes	the
T10337	10766-10769	NN	denotes	Crx
T10338	10770-10780	NN	denotes	expression
T10336	10781-10787	NN	denotes	vector
T10339	10788-10791	VBD	denotes	was
T10334	10792-10805	VBN	denotes	co-introduced
T10340	10806-10810	IN	denotes	with
T10341	10811-10818	NN	denotes	mut1259
T10342	10818-10820	,	denotes	,
T10343	10820-10826	NN	denotes	mut198
T10344	10826-10828	,	denotes	,
T10345	10828-10833	NN	denotes	mut72
T10346	10834-10837	CC	denotes	and
T10347	10838-10841	NN	denotes	mut
T10348	10842-10845	NN	denotes	all
T10349	10845-10847	,	denotes	,
T10350	10847-10859	RB	denotes	respectively
T10351	10860-10861	-LRB-	denotes	(
T10353	10861-10865	NN	denotes	Fig.
T10352	10866-10868	NN	denotes	3F
T10354	10868-10869	-RRB-	denotes	)
T10355	10869-10870	.	denotes	.
T10356	10870-10965	sentence	denotes	The transactivaton activity by Crx was clearly reduced when mut198 or mut72 was co-introduced.
T10357	10871-10874	DT	denotes	The
T10359	10875-10889	NN	denotes	transactivaton
T10358	10890-10898	NN	denotes	activity
T10361	10899-10901	IN	denotes	by
T10362	10902-10905	NN	denotes	Crx
T10363	10906-10909	VBD	denotes	was
T10364	10910-10917	RB	denotes	clearly
T10360	10918-10925	VBN	denotes	reduced
T10365	10926-10930	WRB	denotes	when
T10367	10931-10937	NN	denotes	mut198
T10368	10938-10940	CC	denotes	or
T10369	10941-10946	NN	denotes	mut72
T10370	10947-10950	VBD	denotes	was
T10366	10951-10964	VBN	denotes	co-introduced
T10371	10964-10965	.	denotes	.
T10372	10965-11070	sentence	denotes	On the other hand, when mut1259 was co-transfected, the transactivation activity by Crx was not altered.
T10373	10966-10968	IN	denotes	On
T10375	10969-10972	DT	denotes	the
T10377	10973-10978	JJ	denotes	other
T10376	10979-10983	NN	denotes	hand
T10378	10983-10985	,	denotes	,
T10379	10985-10989	WRB	denotes	when
T10381	10990-10997	NN	denotes	mut1259
T10382	10998-11001	VBD	denotes	was
T10380	11002-11016	VBN	denotes	co-transfected
T10383	11016-11018	,	denotes	,
T10384	11018-11021	DT	denotes	the
T10386	11022-11037	NN	denotes	transactivation
T10385	11038-11046	NN	denotes	activity
T10387	11047-11049	IN	denotes	by
T10388	11050-11053	NN	denotes	Crx
T10389	11054-11057	VBD	denotes	was
T10390	11058-11061	RB	denotes	not
T10374	11062-11069	VBN	denotes	altered
T10391	11069-11070	.	denotes	.
T10392	11070-11252	sentence	denotes	These results suggest that the Crx binding sites 72-bp and 198-bp upstream from the transcription initiation site are crucial for the direct regulation of mr-s transcription by Crx.
T10393	11071-11076	DT	denotes	These
T10394	11077-11084	NNS	denotes	results
T10395	11085-11092	VBP	denotes	suggest
T10396	11093-11097	IN	denotes	that
T10398	11098-11101	DT	denotes	the
T10400	11102-11105	NN	denotes	Crx
T10401	11106-11113	NN	denotes	binding
T10399	11114-11119	NNS	denotes	sites
T10402	11120-11122	CD	denotes	72
T10404	11122-11123	HYPH	denotes	-
T10403	11123-11125	NN	denotes	bp
T10406	11126-11129	CC	denotes	and
T10407	11130-11133	CD	denotes	198
T10409	11133-11134	HYPH	denotes	-
T10408	11134-11136	NN	denotes	bp
T10405	11137-11145	RB	denotes	upstream
T10410	11146-11150	IN	denotes	from
T10411	11151-11154	DT	denotes	the
T10413	11155-11168	NN	denotes	transcription
T10414	11169-11179	NN	denotes	initiation
T10412	11180-11184	NN	denotes	site
T10397	11185-11188	VBP	denotes	are
T10415	11189-11196	JJ	denotes	crucial
T10416	11197-11200	IN	denotes	for
T10417	11201-11204	DT	denotes	the
T10419	11205-11211	JJ	denotes	direct
T10418	11212-11222	NN	denotes	regulation
T10420	11223-11225	IN	denotes	of
T10421	11226-11228	NN	denotes	mr
T10423	11228-11229	HYPH	denotes	-
T10422	11229-11230	NN	denotes	s
T10424	11231-11244	NN	denotes	transcription
T10425	11245-11247	IN	denotes	by
T10426	11248-11251	NN	denotes	Crx
T10427	11251-11252	.	denotes	.
T12462	11254-11258	NN	denotes	Self
T12464	11258-11259	HYPH	denotes	-
T12463	11259-11270	NN	denotes	association
T12465	11271-11273	IN	denotes	of
T12466	11274-11276	NN	denotes	mr
T12468	11276-11277	HYPH	denotes	-
T12467	11277-11278	NN	denotes	s
T12469	11279-11286	NN	denotes	protein
T12470	11286-11368	sentence	denotes	SAM domains are known to function as protein-protein interaction modules [15-17].
T12471	11287-11290	NN	denotes	SAM
T12472	11291-11298	NNS	denotes	domains
T12474	11299-11302	VBP	denotes	are
T12473	11303-11308	VBN	denotes	known
T12475	11309-11311	TO	denotes	to
T12476	11312-11320	VB	denotes	function
T12477	11321-11323	IN	denotes	as
T12478	11324-11331	NN	denotes	protein
T12480	11331-11332	HYPH	denotes	-
T12479	11332-11339	NN	denotes	protein
T12482	11340-11351	NN	denotes	interaction
T12481	11352-11359	NNS	denotes	modules
T12483	11360-11361	-LRB-	denotes	[
T12484	11361-11363	CD	denotes	15
T12485	11363-11364	SYM	denotes	-
T12486	11364-11366	CD	denotes	17
T12487	11366-11367	-RRB-	denotes	]
T12488	11367-11368	.	denotes	.
T12489	11368-11514	sentence	denotes	Although SAM domains can bind to various non-SAM domain-containing proteins, many homo-SAM and hetero-SAM domain interactions have been reported.
T12490	11369-11377	IN	denotes	Although
T12492	11378-11381	NN	denotes	SAM
T12493	11382-11389	NNS	denotes	domains
T12494	11390-11393	MD	denotes	can
T12491	11394-11398	VB	denotes	bind
T12496	11399-11401	IN	denotes	to
T12497	11402-11409	JJ	denotes	various
T12499	11410-11417	JJ	denotes	non-SAM
T12500	11418-11424	NN	denotes	domain
T12502	11424-11425	HYPH	denotes	-
T12501	11425-11435	VBG	denotes	containing
T12498	11436-11444	NN	denotes	proteins
T12503	11444-11446	,	denotes	,
T12504	11446-11450	JJ	denotes	many
T12506	11451-11459	NN	denotes	homo-SAM
T12507	11460-11463	CC	denotes	and
T12508	11464-11474	NN	denotes	hetero-SAM
T12509	11475-11481	NN	denotes	domain
T12505	11482-11494	NNS	denotes	interactions
T12510	11495-11499	VBP	denotes	have
T12511	11500-11504	VBN	denotes	been
T12495	11505-11513	VBN	denotes	reported
T12512	11513-11514	.	denotes	.
T12513	11514-11715	sentence	denotes	To investigate whether the SAM domain of the mr-s protein can also function as a protein-protein interaction module, we performed yeast two-hybrid screening using full-length mr-s protein as the bait.
T12514	11515-11517	TO	denotes	To
T12515	11518-11529	VB	denotes	investigate
T12517	11530-11537	IN	denotes	whether
T12519	11538-11541	DT	denotes	the
T12521	11542-11545	NN	denotes	SAM
T12520	11546-11552	NN	denotes	domain
T12522	11553-11555	IN	denotes	of
T12523	11556-11559	DT	denotes	the
T12525	11560-11562	NN	denotes	mr
T12527	11562-11563	HYPH	denotes	-
T12526	11563-11564	NN	denotes	s
T12524	11565-11572	NN	denotes	protein
T12528	11573-11576	MD	denotes	can
T12529	11577-11581	RB	denotes	also
T12518	11582-11590	VB	denotes	function
T12530	11591-11593	IN	denotes	as
T12531	11594-11595	DT	denotes	a
T12533	11596-11603	NN	denotes	protein
T12535	11603-11604	HYPH	denotes	-
T12534	11604-11611	NN	denotes	protein
T12536	11612-11623	NN	denotes	interaction
T12532	11624-11630	NN	denotes	module
T12537	11630-11632	,	denotes	,
T12538	11632-11634	PRP	denotes	we
T12516	11635-11644	VBD	denotes	performed
T12539	11645-11650	NN	denotes	yeast
T12541	11651-11654	CD	denotes	two
T12543	11654-11655	HYPH	denotes	-
T12542	11655-11661	NN	denotes	hybrid
T12540	11662-11671	NN	denotes	screening
T12544	11672-11677	VBG	denotes	using
T12545	11678-11682	JJ	denotes	full
T12547	11682-11683	HYPH	denotes	-
T12546	11683-11689	NN	denotes	length
T12549	11690-11692	NN	denotes	mr
T12551	11692-11693	HYPH	denotes	-
T12550	11693-11694	NN	denotes	s
T12548	11695-11702	NN	denotes	protein
T12552	11703-11705	IN	denotes	as
T12553	11706-11709	DT	denotes	the
T12554	11710-11714	NN	denotes	bait
T12555	11714-11715	.	denotes	.
T12556	11715-11874	sentence	denotes	Using this bait, we screened the transcriptional activator fusion protein library in which mouse P0-P3 retinal cDNAs were fused to the GAL4 activation domain.
T12557	11716-11721	VBG	denotes	Using
T12559	11722-11726	DT	denotes	this
T12560	11727-11731	NN	denotes	bait
T12561	11731-11733	,	denotes	,
T12562	11733-11735	PRP	denotes	we
T12558	11736-11744	VBD	denotes	screened
T12563	11745-11748	DT	denotes	the
T12565	11749-11764	JJ	denotes	transcriptional
T12566	11765-11774	NN	denotes	activator
T12568	11775-11781	NN	denotes	fusion
T12567	11782-11789	NN	denotes	protein
T12564	11790-11797	NN	denotes	library
T12569	11798-11800	IN	denotes	in
T12571	11801-11806	WDT	denotes	which
T12572	11807-11812	NN	denotes	mouse
T12574	11813-11815	NN	denotes	P0
T12575	11815-11816	SYM	denotes	-
T12576	11816-11818	NN	denotes	P3
T12577	11819-11826	JJ	denotes	retinal
T12573	11827-11832	NNS	denotes	cDNAs
T12578	11833-11837	VBD	denotes	were
T12570	11838-11843	VBN	denotes	fused
T12579	11844-11846	IN	denotes	to
T12580	11847-11850	DT	denotes	the
T12582	11851-11855	NN	denotes	GAL4
T12583	11856-11866	NN	denotes	activation
T12581	11867-11873	NN	denotes	domain
T12584	11873-11874	.	denotes	.
T12585	11874-11987	sentence	denotes	The most frequent positive clones (5 out of 28) were cDNA fragments containing the SAM domain of mr-s (Fig. 4A).
T12586	11875-11878	DT	denotes	The
T12588	11879-11883	RBS	denotes	most
T12589	11884-11892	JJ	denotes	frequent
T12590	11893-11901	JJ	denotes	positive
T12587	11902-11908	NNS	denotes	clones
T12592	11909-11910	-LRB-	denotes	(
T12594	11910-11911	CD	denotes	5
T12595	11912-11915	IN	denotes	out
T12596	11916-11918	IN	denotes	of
T12593	11919-11921	CD	denotes	28
T12597	11921-11922	-RRB-	denotes	)
T12591	11923-11927	VBD	denotes	were
T12598	11928-11932	NN	denotes	cDNA
T12599	11933-11942	NNS	denotes	fragments
T12600	11943-11953	VBG	denotes	containing
T12601	11954-11957	DT	denotes	the
T12603	11958-11961	NN	denotes	SAM
T12602	11962-11968	NN	denotes	domain
T12604	11969-11971	IN	denotes	of
T12605	11972-11974	NN	denotes	mr
T12607	11974-11975	HYPH	denotes	-
T12606	11975-11976	NN	denotes	s
T12608	11977-11978	-LRB-	denotes	(
T12610	11978-11982	NN	denotes	Fig.
T12609	11983-11985	NN	denotes	4A
T12611	11985-11986	-RRB-	denotes	)
T12612	11986-11987	.	denotes	.
T12613	11987-12090	sentence	denotes	This result strongly suggests that mr-s protein self-associates through SAM domain-containing regions.
T12614	11988-11992	DT	denotes	This
T12615	11993-11999	NN	denotes	result
T12617	12000-12008	RB	denotes	strongly
T12616	12009-12017	VBZ	denotes	suggests
T12618	12018-12022	IN	denotes	that
T12620	12023-12025	NN	denotes	mr
T12622	12025-12026	HYPH	denotes	-
T12621	12026-12027	NN	denotes	s
T12623	12028-12035	NN	denotes	protein
T12624	12036-12040	NN	denotes	self
T12625	12040-12041	HYPH	denotes	-
T12619	12041-12051	VBZ	denotes	associates
T12626	12052-12059	IN	denotes	through
T12627	12060-12063	NN	denotes	SAM
T12628	12064-12070	NN	denotes	domain
T12630	12070-12071	HYPH	denotes	-
T12629	12071-12081	VBG	denotes	containing
T12631	12082-12089	NNS	denotes	regions
T12632	12089-12090	.	denotes	.
T12633	12090-12162	sentence	denotes	We then directly tested this self-association of mr-s protein in yeast.
T12634	12091-12093	PRP	denotes	We
T12636	12094-12098	RB	denotes	then
T12637	12099-12107	RB	denotes	directly
T12635	12108-12114	VBN	denotes	tested
T12638	12115-12119	DT	denotes	this
T12640	12120-12124	NN	denotes	self
T12641	12124-12125	HYPH	denotes	-
T12639	12125-12136	NN	denotes	association
T12642	12137-12139	IN	denotes	of
T12643	12140-12142	NN	denotes	mr
T12645	12142-12143	HYPH	denotes	-
T12644	12143-12144	NN	denotes	s
T12646	12145-12152	NN	denotes	protein
T12647	12153-12155	IN	denotes	in
T12648	12156-12161	NN	denotes	yeast
T12649	12161-12162	.	denotes	.
T12650	12162-12315	sentence	denotes	We fused full-length or truncated portions of the mr-s protein to the DNA-binding domain of the yeast transcription factor GAL4 to make bait constructs.
T12651	12163-12165	PRP	denotes	We
T12652	12166-12171	VBD	denotes	fused
T12653	12172-12176	JJ	denotes	full
T12655	12176-12177	HYPH	denotes	-
T12654	12177-12183	NN	denotes	length
T12657	12184-12186	CC	denotes	or
T12658	12187-12196	VBN	denotes	truncated
T12656	12197-12205	NNS	denotes	portions
T12659	12206-12208	IN	denotes	of
T12660	12209-12212	DT	denotes	the
T12662	12213-12215	NN	denotes	mr
T12664	12215-12216	HYPH	denotes	-
T12663	12216-12217	NN	denotes	s
T12661	12218-12225	NN	denotes	protein
T12665	12226-12228	IN	denotes	to
T12666	12229-12232	DT	denotes	the
T12668	12233-12236	NN	denotes	DNA
T12670	12236-12237	HYPH	denotes	-
T12669	12237-12244	VBG	denotes	binding
T12667	12245-12251	NN	denotes	domain
T12671	12252-12254	IN	denotes	of
T12672	12255-12258	DT	denotes	the
T12674	12259-12264	NN	denotes	yeast
T12675	12265-12278	NN	denotes	transcription
T12673	12279-12285	NN	denotes	factor
T12676	12286-12290	NN	denotes	GAL4
T12677	12291-12293	TO	denotes	to
T12678	12294-12298	VB	denotes	make
T12679	12299-12303	NN	denotes	bait
T12680	12304-12314	NNS	denotes	constructs
T12681	12314-12315	.	denotes	.
T12682	12315-12459	sentence	denotes	We fused full-length or truncated portions of the mr-s protein to the GAL4 transcriptional activation domain to make prey constructs (Fig. 4B).
T12683	12316-12318	PRP	denotes	We
T12684	12319-12324	VBD	denotes	fused
T12685	12325-12329	JJ	denotes	full
T12687	12329-12330	HYPH	denotes	-
T12686	12330-12336	NN	denotes	length
T12689	12337-12339	CC	denotes	or
T12690	12340-12349	VBN	denotes	truncated
T12688	12350-12358	NNS	denotes	portions
T12691	12359-12361	IN	denotes	of
T12692	12362-12365	DT	denotes	the
T12694	12366-12368	NN	denotes	mr
T12696	12368-12369	HYPH	denotes	-
T12695	12369-12370	NN	denotes	s
T12693	12371-12378	NN	denotes	protein
T12697	12379-12381	IN	denotes	to
T12698	12382-12385	DT	denotes	the
T12700	12386-12390	NN	denotes	GAL4
T12702	12391-12406	JJ	denotes	transcriptional
T12701	12407-12417	NN	denotes	activation
T12699	12418-12424	NN	denotes	domain
T12703	12425-12427	TO	denotes	to
T12704	12428-12432	VB	denotes	make
T12705	12433-12437	NN	denotes	prey
T12706	12438-12448	NNS	denotes	constructs
T12707	12449-12450	-LRB-	denotes	(
T12709	12450-12454	NN	denotes	Fig.
T12708	12455-12457	NN	denotes	4B
T12710	12457-12458	-RRB-	denotes	)
T12711	12458-12459	.	denotes	.
T12712	12459-12580	sentence	denotes	These constructs were transformed into yeast that contain a transgene with GAL4 binding sites upstream of the lacZ gene.
T12713	12460-12465	DT	denotes	These
T12714	12466-12476	NNS	denotes	constructs
T12716	12477-12481	VBD	denotes	were
T12715	12482-12493	VBN	denotes	transformed
T12717	12494-12498	IN	denotes	into
T12718	12499-12504	NN	denotes	yeast
T12719	12505-12509	WDT	denotes	that
T12720	12510-12517	VBP	denotes	contain
T12721	12518-12519	DT	denotes	a
T12722	12520-12529	NN	denotes	transgene
T12723	12530-12534	IN	denotes	with
T12724	12535-12539	NN	denotes	GAL4
T12725	12540-12547	NN	denotes	binding
T12726	12548-12553	NNS	denotes	sites
T12727	12554-12562	RB	denotes	upstream
T12728	12563-12565	IN	denotes	of
T12729	12566-12569	DT	denotes	the
T12731	12570-12574	NN	denotes	lacZ
T12730	12575-12579	NN	denotes	gene
T12732	12579-12580	.	denotes	.
T12733	12580-12719	sentence	denotes	We found that the full-length mr-s bait construct induced lacZ expression with the full-length mr-s prey construct (Fig. 4B, full × full).
T12734	12581-12583	PRP	denotes	We
T12735	12584-12589	VBD	denotes	found
T12736	12590-12594	IN	denotes	that
T12738	12595-12598	DT	denotes	the
T12740	12599-12603	JJ	denotes	full
T12742	12603-12604	HYPH	denotes	-
T12741	12604-12610	NN	denotes	length
T12743	12611-12613	NN	denotes	mr
T12745	12613-12614	HYPH	denotes	-
T12744	12614-12615	NN	denotes	s
T12746	12616-12620	NN	denotes	bait
T12739	12621-12630	NN	denotes	construct
T12737	12631-12638	VBD	denotes	induced
T12747	12639-12643	NN	denotes	lacZ
T12748	12644-12654	NN	denotes	expression
T12749	12655-12659	IN	denotes	with
T12750	12660-12663	DT	denotes	the
T12752	12664-12668	JJ	denotes	full
T12754	12668-12669	HYPH	denotes	-
T12753	12669-12675	NN	denotes	length
T12755	12676-12678	NN	denotes	mr
T12757	12678-12679	HYPH	denotes	-
T12756	12679-12680	NN	denotes	s
T12758	12681-12685	NN	denotes	prey
T12751	12686-12695	NN	denotes	construct
T12759	12696-12697	-LRB-	denotes	(
T12761	12697-12701	NN	denotes	Fig.
T12762	12702-12704	NN	denotes	4B
T12763	12704-12706	,	denotes	,
T12764	12706-12710	JJ	denotes	full
T12765	12711-12712	SYM	denotes	×
T12760	12713-12717	JJ	denotes	full
T12766	12717-12718	-RRB-	denotes	)
T12767	12718-12719	.	denotes	.
T12768	12719-12872	sentence	denotes	The N-terminus 400 amino acid (aa) stretch of mr-s, which does not contain a SAM domain, does weakly activate transcription of lacZ (Fig. 4B, full × N).
T12769	12720-12723	DT	denotes	The
T12771	12724-12725	NN	denotes	N
T12773	12725-12726	HYPH	denotes	-
T12772	12726-12734	NN	denotes	terminus
T12774	12735-12738	CD	denotes	400
T12775	12739-12744	NN	denotes	amino
T12776	12745-12749	NN	denotes	acid
T12777	12750-12751	-LRB-	denotes	(
T12778	12751-12753	NN	denotes	aa
T12779	12753-12754	-RRB-	denotes	)
T12770	12755-12762	NN	denotes	stretch
T12781	12763-12765	IN	denotes	of
T12782	12766-12768	NN	denotes	mr
T12784	12768-12769	HYPH	denotes	-
T12783	12769-12770	NN	denotes	s
T12785	12770-12772	,	denotes	,
T12786	12772-12777	WDT	denotes	which
T12788	12778-12782	VBZ	denotes	does
T12789	12783-12786	RB	denotes	not
T12787	12787-12794	VB	denotes	contain
T12790	12795-12796	DT	denotes	a
T12792	12797-12800	NN	denotes	SAM
T12791	12801-12807	NN	denotes	domain
T12793	12807-12809	,	denotes	,
T12794	12809-12813	VBZ	denotes	does
T12795	12814-12820	RB	denotes	weakly
T12780	12821-12829	VB	denotes	activate
T12796	12830-12843	NN	denotes	transcription
T12797	12844-12846	IN	denotes	of
T12798	12847-12851	NN	denotes	lacZ
T12799	12852-12853	-LRB-	denotes	(
T12801	12853-12857	NN	denotes	Fig.
T12802	12858-12860	NN	denotes	4B
T12803	12860-12862	,	denotes	,
T12804	12862-12866	JJ	denotes	full
T12805	12867-12868	SYM	denotes	×
T12800	12869-12870	NN	denotes	N
T12806	12870-12871	-RRB-	denotes	)
T12807	12871-12872	.	denotes	.
T12808	12872-13024	sentence	denotes	The N-terminus 400 aa stretch of mr-s was able to induce transcription of lacZ weakly with the same N-terminus 400 aa stretch of mr-s (Fig. 4B, N × N).
T12809	12873-12876	DT	denotes	The
T12811	12877-12878	NN	denotes	N
T12813	12878-12879	HYPH	denotes	-
T12812	12879-12887	NN	denotes	terminus
T12814	12888-12891	CD	denotes	400
T12815	12892-12894	NN	denotes	aa
T12810	12895-12902	NN	denotes	stretch
T12817	12903-12905	IN	denotes	of
T12818	12906-12908	NN	denotes	mr
T12820	12908-12909	HYPH	denotes	-
T12819	12909-12910	NN	denotes	s
T12816	12911-12914	VBD	denotes	was
T12821	12915-12919	JJ	denotes	able
T12822	12920-12922	TO	denotes	to
T12823	12923-12929	VB	denotes	induce
T12824	12930-12943	NN	denotes	transcription
T12825	12944-12946	IN	denotes	of
T12826	12947-12951	NN	denotes	lacZ
T12827	12952-12958	RB	denotes	weakly
T12828	12959-12963	IN	denotes	with
T12829	12964-12967	DT	denotes	the
T12831	12968-12972	JJ	denotes	same
T12832	12973-12974	NN	denotes	N
T12834	12974-12975	HYPH	denotes	-
T12833	12975-12983	NN	denotes	terminus
T12835	12984-12987	CD	denotes	400
T12836	12988-12990	NN	denotes	aa
T12830	12991-12998	NN	denotes	stretch
T12837	12999-13001	IN	denotes	of
T12838	13002-13004	NN	denotes	mr
T12840	13004-13005	HYPH	denotes	-
T12839	13005-13006	NN	denotes	s
T12841	13007-13008	-LRB-	denotes	(
T12843	13008-13012	NN	denotes	Fig.
T12844	13013-13015	NN	denotes	4B
T12845	13015-13017	,	denotes	,
T12846	13017-13018	NN	denotes	N
T12847	13019-13020	SYM	denotes	×
T12842	13021-13022	NN	denotes	N
T12848	13022-13023	-RRB-	denotes	)
T12849	13023-13024	.	denotes	.
T12850	13024-13291	sentence	denotes	Although the N-terminus 400 aa mr-s protein weakly activates lacZ transcription with the same N-terminus portion, a much stronger activation of lacZ expression was observed with a C-terminus portion encoding the 391–542 aa stretch of mr-s (Fig. 4B, full × C, C × C).
T12851	13025-13033	IN	denotes	Although
T12853	13034-13037	DT	denotes	the
T12855	13038-13039	NN	denotes	N
T12857	13039-13040	HYPH	denotes	-
T12856	13040-13048	NN	denotes	terminus
T12858	13049-13052	CD	denotes	400
T12859	13053-13055	NN	denotes	aa
T12860	13056-13058	NN	denotes	mr
T12862	13058-13059	HYPH	denotes	-
T12861	13059-13060	NN	denotes	s
T12854	13061-13068	NN	denotes	protein
T12863	13069-13075	RB	denotes	weakly
T12852	13076-13085	VBZ	denotes	activates
T12865	13086-13090	NN	denotes	lacZ
T12866	13091-13104	NN	denotes	transcription
T12867	13105-13109	IN	denotes	with
T12868	13110-13113	DT	denotes	the
T12870	13114-13118	JJ	denotes	same
T12871	13119-13120	NN	denotes	N
T12873	13120-13121	HYPH	denotes	-
T12872	13121-13129	NN	denotes	terminus
T12869	13130-13137	NN	denotes	portion
T12874	13137-13139	,	denotes	,
T12875	13139-13140	DT	denotes	a
T12877	13141-13145	RB	denotes	much
T12878	13146-13154	JJR	denotes	stronger
T12876	13155-13165	NN	denotes	activation
T12879	13166-13168	IN	denotes	of
T12880	13169-13173	NN	denotes	lacZ
T12881	13174-13184	NN	denotes	expression
T12882	13185-13188	VBD	denotes	was
T12864	13189-13197	VBN	denotes	observed
T12883	13198-13202	IN	denotes	with
T12884	13203-13204	DT	denotes	a
T12886	13205-13206	NN	denotes	C
T12888	13206-13207	HYPH	denotes	-
T12887	13207-13215	NN	denotes	terminus
T12885	13216-13223	NN	denotes	portion
T12889	13224-13232	VBG	denotes	encoding
T12890	13233-13236	DT	denotes	the
T12892	13237-13240	CD	denotes	391
T12894	13240-13241	SYM	denotes	–
T12895	13241-13244	CD	denotes	542
T12893	13245-13247	NN	denotes	aa
T12891	13248-13255	NN	denotes	stretch
T12896	13256-13258	IN	denotes	of
T12897	13259-13261	NN	denotes	mr
T12899	13261-13262	HYPH	denotes	-
T12898	13262-13263	NN	denotes	s
T12900	13264-13265	-LRB-	denotes	(
T12902	13265-13269	NN	denotes	Fig.
T12903	13270-13272	NN	denotes	4B
T12904	13272-13274	,	denotes	,
T12905	13274-13278	JJ	denotes	full
T12906	13279-13280	SYM	denotes	×
T12901	13281-13282	NN	denotes	C
T12907	13282-13284	,	denotes	,
T12908	13284-13285	NN	denotes	C
T12910	13286-13287	SYM	denotes	×
T12909	13288-13289	NN	denotes	C
T12911	13289-13290	-RRB-	denotes	)
T12912	13290-13291	.	denotes	.
T12913	13291-13458	sentence	denotes	Our GAL4 assay indicated that the signal when the full-length mr-s was present in both the bait and prey contexts was weaker than when isolated SAM domains were used.
T12914	13292-13295	PRP$	denotes	Our
T12916	13296-13300	NN	denotes	GAL4
T12915	13301-13306	NN	denotes	assay
T12917	13307-13316	VBD	denotes	indicated
T12918	13317-13321	IN	denotes	that
T12920	13322-13325	DT	denotes	the
T12921	13326-13332	NN	denotes	signal
T12922	13333-13337	WRB	denotes	when
T12924	13338-13341	DT	denotes	the
T12926	13342-13346	JJ	denotes	full
T12928	13346-13347	HYPH	denotes	-
T12927	13347-13353	NN	denotes	length
T12929	13354-13356	NN	denotes	mr
T12930	13356-13357	HYPH	denotes	-
T12925	13357-13358	NN	denotes	s
T12923	13359-13362	VBD	denotes	was
T12931	13363-13370	JJ	denotes	present
T12932	13371-13373	IN	denotes	in
T12933	13374-13378	CC	denotes	both
T12935	13379-13382	DT	denotes	the
T12936	13383-13387	NN	denotes	bait
T12937	13388-13391	CC	denotes	and
T12938	13392-13396	NN	denotes	prey
T12934	13397-13405	NNS	denotes	contexts
T12919	13406-13409	VBD	denotes	was
T12939	13410-13416	JJR	denotes	weaker
T12940	13417-13421	IN	denotes	than
T12941	13422-13426	WRB	denotes	when
T12943	13427-13435	JJ	denotes	isolated
T12945	13436-13439	NN	denotes	SAM
T12944	13440-13447	NNS	denotes	domains
T12946	13448-13452	VBD	denotes	were
T12942	13453-13457	VBN	denotes	used
T12947	13457-13458	.	denotes	.
T12948	13458-13583	sentence	denotes	This may simply reflect the tendency for the small fusion proteins to enter the yeast nucleus and occupy GAL4 binding sites.
T12949	13459-13463	DT	denotes	This
T12951	13464-13467	MD	denotes	may
T12952	13468-13474	RB	denotes	simply
T12950	13475-13482	VB	denotes	reflect
T12953	13483-13486	DT	denotes	the
T12954	13487-13495	NN	denotes	tendency
T12955	13496-13499	IN	denotes	for
T12957	13500-13503	DT	denotes	the
T12959	13504-13509	JJ	denotes	small
T12960	13510-13516	NN	denotes	fusion
T12958	13517-13525	NN	denotes	proteins
T12961	13526-13528	TO	denotes	to
T12956	13529-13534	VB	denotes	enter
T12962	13535-13538	DT	denotes	the
T12964	13539-13544	NN	denotes	yeast
T12963	13545-13552	NN	denotes	nucleus
T12965	13553-13556	CC	denotes	and
T12966	13557-13563	VB	denotes	occupy
T12967	13564-13568	NN	denotes	GAL4
T12968	13569-13576	NN	denotes	binding
T12969	13577-13582	NNS	denotes	sites
T12970	13582-13583	.	denotes	.
T12971	13583-13716	sentence	denotes	Alternatively, the SAM domain may be less accessible for interaction in the full-length protein context as previously reported [39].
T12972	13584-13597	RB	denotes	Alternatively
T12974	13597-13599	,	denotes	,
T12975	13599-13602	DT	denotes	the
T12977	13603-13606	NN	denotes	SAM
T12976	13607-13613	NN	denotes	domain
T12978	13614-13617	MD	denotes	may
T12973	13618-13620	VB	denotes	be
T12979	13621-13625	RBR	denotes	less
T12980	13626-13636	JJ	denotes	accessible
T12981	13637-13640	IN	denotes	for
T12982	13641-13652	NN	denotes	interaction
T12983	13653-13655	IN	denotes	in
T12984	13656-13659	DT	denotes	the
T12986	13660-13664	JJ	denotes	full
T12988	13664-13665	HYPH	denotes	-
T12987	13665-13671	NN	denotes	length
T12989	13672-13679	NN	denotes	protein
T12985	13680-13687	NN	denotes	context
T12990	13688-13690	IN	denotes	as
T12992	13691-13701	RB	denotes	previously
T12991	13702-13710	VBN	denotes	reported
T12993	13711-13712	-LRB-	denotes	[
T12994	13712-13714	CD	denotes	39
T12995	13714-13715	-RRB-	denotes	]
T12996	13715-13716	.	denotes	.
T12997	13716-13717	sentence	denotes
T30892	13727-13734	NN	denotes	Summary
T30893	13735-13737	IN	denotes	of
T30894	13738-13743	NN	denotes	yeast
T30896	13744-13747	CD	denotes	two
T30898	13747-13748	HYPH	denotes	-
T30897	13748-13754	NN	denotes	hybrid
T30895	13755-13764	NN	denotes	screening
T30899	13765-13768	CC	denotes	and
T30900	13769-13773	NN	denotes	GAL4
T30901	13774-13779	NN	denotes	assay
T30902	13779-13780	.	denotes	.
T30903	13780-13885	sentence	denotes	(A) Full-length mr-s as a bait used in the yeast two-hybrid screening and positive clones are indicated.
T30904	13781-13782	-LRB-	denotes	(
T30905	13782-13783	LS	denotes	A
T30907	13783-13784	-RRB-	denotes	)
T30908	13785-13789	JJ	denotes	Full
T30910	13789-13790	HYPH	denotes	-
T30909	13790-13796	NN	denotes	length
T30912	13797-13799	NN	denotes	mr
T30913	13799-13800	HYPH	denotes	-
T30911	13800-13801	NN	denotes	s
T30914	13802-13804	IN	denotes	as
T30915	13805-13806	DT	denotes	a
T30916	13807-13811	NN	denotes	bait
T30917	13812-13816	VBN	denotes	used
T30918	13817-13819	IN	denotes	in
T30919	13820-13823	DT	denotes	the
T30921	13824-13829	NN	denotes	yeast
T30922	13830-13833	CD	denotes	two
T30924	13833-13834	HYPH	denotes	-
T30923	13834-13840	NN	denotes	hybrid
T30920	13841-13850	NN	denotes	screening
T30925	13851-13854	CC	denotes	and
T30926	13855-13863	JJ	denotes	positive
T30927	13864-13870	NNS	denotes	clones
T30928	13871-13874	VBP	denotes	are
T30906	13875-13884	VBN	denotes	indicated
T30929	13884-13885	.	denotes	.
T30930	13885-13968	sentence	denotes	Note that all of five mr-s clones identified in the screening contain SAM domains.
T30931	13886-13890	VB	denotes	Note
T30932	13891-13895	IN	denotes	that
T30934	13896-13899	DT	denotes	all
T30935	13900-13902	IN	denotes	of
T30936	13903-13907	CD	denotes	five
T30938	13908-13910	NN	denotes	mr
T30940	13910-13911	HYPH	denotes	-
T30939	13911-13912	NN	denotes	s
T30937	13913-13919	NNS	denotes	clones
T30941	13920-13930	VBN	denotes	identified
T30942	13931-13933	IN	denotes	in
T30943	13934-13937	DT	denotes	the
T30944	13938-13947	NN	denotes	screening
T30933	13948-13955	VBP	denotes	contain
T30945	13956-13959	NN	denotes	SAM
T30946	13960-13967	NNS	denotes	domains
T30947	13967-13968	.	denotes	.
T30948	13968-14048	sentence	denotes	(B) Schematic diagram of the mr-s fusion proteins used in the yeast GAL4 assay.
T30949	13969-13970	-LRB-	denotes	(
T30950	13970-13971	LS	denotes	B
T30952	13971-13972	-RRB-	denotes	)
T30953	13973-13982	JJ	denotes	Schematic
T30951	13983-13990	NN	denotes	diagram
T30954	13991-13993	IN	denotes	of
T30955	13994-13997	DT	denotes	the
T30957	13998-14000	NN	denotes	mr
T30959	14000-14001	HYPH	denotes	-
T30958	14001-14002	NN	denotes	s
T30960	14003-14009	NN	denotes	fusion
T30956	14010-14018	NN	denotes	proteins
T30961	14019-14023	VBN	denotes	used
T30962	14024-14026	IN	denotes	in
T30963	14027-14030	DT	denotes	the
T30965	14031-14036	NN	denotes	yeast
T30966	14037-14041	NN	denotes	GAL4
T30964	14042-14047	NN	denotes	assay
T30967	14047-14048	.	denotes	.
T30968	14048-14099	sentence	denotes	Black boxes represent the position of SAM domains.
T30969	14049-14054	JJ	denotes	Black
T30970	14055-14060	NNS	denotes	boxes
T30971	14061-14070	VBP	denotes	represent
T30972	14071-14074	DT	denotes	the
T30973	14075-14083	NN	denotes	position
T30974	14084-14086	IN	denotes	of
T30975	14087-14090	NN	denotes	SAM
T30976	14091-14098	NNS	denotes	domains
T30977	14098-14099	.	denotes	.
T30978	14099-14172	sentence	denotes	Relative levels of LacZ expression are shown on the right, respectively.
T30979	14100-14108	JJ	denotes	Relative
T30980	14109-14115	NNS	denotes	levels
T30982	14116-14118	IN	denotes	of
T30983	14119-14123	NN	denotes	LacZ
T30984	14124-14134	NN	denotes	expression
T30985	14135-14138	VBP	denotes	are
T30981	14139-14144	VBN	denotes	shown
T30986	14145-14147	IN	denotes	on
T30987	14148-14151	DT	denotes	the
T30988	14152-14157	NN	denotes	right
T30989	14157-14159	,	denotes	,
T30990	14159-14171	RB	denotes	respectively
T30991	14171-14172	.	denotes	.
T30992	14172-14316	sentence	denotes	Note; ++ indicates an intense blue signal visible after 12hr of incubation at 37°C, + indicates a blue signal visible after 24hr of incubation.
T30993	14173-14177	NN	denotes	Note
T30995	14177-14178	:	denotes	;
T30996	14179-14181	SYM	denotes	++
T30997	14182-14191	VBZ	denotes	indicates
T30998	14192-14194	DT	denotes	an
T31000	14195-14202	JJ	denotes	intense
T31001	14203-14207	JJ	denotes	blue
T30999	14208-14214	NN	denotes	signal
T31002	14215-14222	JJ	denotes	visible
T31003	14223-14228	IN	denotes	after
T31004	14229-14233	NN	denotes	12hr
T31005	14234-14236	IN	denotes	of
T31006	14237-14247	NN	denotes	incubation
T31007	14248-14250	IN	denotes	at
T31008	14251-14253	CD	denotes	37
T31009	14253-14255	NN	denotes	°C
T31010	14255-14257	,	denotes	,
T31011	14257-14258	SYM	denotes	+
T30994	14259-14268	VBZ	denotes	indicates
T31012	14269-14270	DT	denotes	a
T31014	14271-14275	JJ	denotes	blue
T31013	14276-14282	NN	denotes	signal
T31015	14283-14290	JJ	denotes	visible
T31016	14291-14296	IN	denotes	after
T31017	14297-14301	NN	denotes	24hr
T31018	14302-14304	IN	denotes	of
T31019	14305-14315	NN	denotes	incubation
T31020	14315-14316	.	denotes	.
T31021	14316-14493	sentence	denotes	BD, binding domain; AD, activation domain; full, full-length mr-s; N, N-terminal portion of mr-s (amino acids 1 to 400); C, C-terminal portion of mr-s (amino acids 391 to 542).
T31022	14317-14319	NN	denotes	BD
T31023	14319-14321	,	denotes	,
T31024	14321-14328	NN	denotes	binding
T31025	14329-14335	NN	denotes	domain
T31026	14335-14336	:	denotes	;
T31027	14337-14339	NN	denotes	AD
T31028	14339-14341	,	denotes	,
T31029	14341-14351	NN	denotes	activation
T31030	14352-14358	NN	denotes	domain
T31031	14358-14359	:	denotes	;
T31032	14360-14364	JJ	denotes	full
T31034	14364-14366	,	denotes	,
T31035	14366-14370	JJ	denotes	full
T31037	14370-14371	HYPH	denotes	-
T31036	14371-14377	NN	denotes	length
T31038	14378-14380	NN	denotes	mr
T31039	14380-14381	HYPH	denotes	-
T31033	14381-14382	NN	denotes	s
T31040	14382-14383	:	denotes	;
T31041	14384-14385	NN	denotes	N
T31042	14385-14387	,	denotes	,
T31043	14387-14388	NN	denotes	N
T31045	14388-14389	HYPH	denotes	-
T31044	14389-14397	JJ	denotes	terminal
T31046	14398-14405	NN	denotes	portion
T31047	14406-14408	IN	denotes	of
T31048	14409-14411	NN	denotes	mr
T31050	14411-14412	HYPH	denotes	-
T31049	14412-14413	NN	denotes	s
T31051	14414-14415	-LRB-	denotes	(
T31053	14415-14420	NN	denotes	amino
T31054	14421-14426	NNS	denotes	acids
T31052	14427-14428	CD	denotes	1
T31055	14429-14431	IN	denotes	to
T31056	14432-14435	CD	denotes	400
T31057	14435-14436	-RRB-	denotes	)
T31058	14436-14437	:	denotes	;
T31059	14438-14439	NN	denotes	C
T31060	14439-14441	,	denotes	,
T31061	14441-14442	NN	denotes	C
T31063	14442-14443	HYPH	denotes	-
T31062	14443-14451	JJ	denotes	terminal
T31064	14452-14459	NN	denotes	portion
T31065	14460-14462	IN	denotes	of
T31066	14463-14465	NN	denotes	mr
T31068	14465-14466	HYPH	denotes	-
T31067	14466-14467	NN	denotes	s
T31069	14468-14469	-LRB-	denotes	(
T31071	14469-14474	NN	denotes	amino
T31072	14475-14480	NNS	denotes	acids
T31070	14481-14484	CD	denotes	391
T31073	14485-14487	IN	denotes	to
T31074	14488-14491	CD	denotes	542
T31075	14491-14492	-RRB-	denotes	)
T31076	14492-14493	.	denotes	.
T12999	14494-14496	TO	denotes	To
T12998	14494-14740	sentence	denotes	To confirm self-association of the mr-s protein in mammalian cells, we next performed co-immunoprecipitation studies in HEK293T cells by co-transfection of HA-tagged full-length/truncated mr-s and Flag-tagged full-length/truncated mr-s (Fig. 5A).
T13000	14497-14504	VB	denotes	confirm
T13002	14505-14509	NN	denotes	self
T13004	14509-14510	HYPH	denotes	-
T13003	14510-14521	NN	denotes	association
T13005	14522-14524	IN	denotes	of
T13006	14525-14528	DT	denotes	the
T13008	14529-14531	NN	denotes	mr
T13010	14531-14532	HYPH	denotes	-
T13009	14532-14533	NN	denotes	s
T13007	14534-14541	NN	denotes	protein
T13011	14542-14544	IN	denotes	in
T13012	14545-14554	JJ	denotes	mammalian
T13013	14555-14560	NNS	denotes	cells
T13014	14560-14562	,	denotes	,
T13015	14562-14564	PRP	denotes	we
T13016	14565-14569	RB	denotes	next
T13001	14570-14579	VBD	denotes	performed
T13017	14580-14602	NN	denotes	co-immunoprecipitation
T13018	14603-14610	NNS	denotes	studies
T13019	14611-14613	IN	denotes	in
T13020	14614-14621	NN	denotes	HEK293T
T13021	14622-14627	NNS	denotes	cells
T13022	14628-14630	IN	denotes	by
T13023	14631-14646	NN	denotes	co-transfection
T13024	14647-14649	IN	denotes	of
T13025	14650-14652	NN	denotes	HA
T13027	14652-14653	HYPH	denotes	-
T13026	14653-14659	VBN	denotes	tagged
T13029	14660-14664	JJ	denotes	full
T13031	14664-14665	HYPH	denotes	-
T13030	14665-14671	NN	denotes	length
T13033	14671-14672	HYPH	denotes	/
T13032	14672-14681	VBN	denotes	truncated
T13034	14682-14684	NN	denotes	mr
T13035	14684-14685	HYPH	denotes	-
T13028	14685-14686	NN	denotes	s
T13036	14687-14690	CC	denotes	and
T13037	14691-14695	NN	denotes	Flag
T13039	14695-14696	HYPH	denotes	-
T13038	14696-14702	VBN	denotes	tagged
T13041	14703-14707	JJ	denotes	full
T13043	14707-14708	HYPH	denotes	-
T13042	14708-14714	NN	denotes	length
T13045	14714-14715	HYPH	denotes	/
T13044	14715-14724	VBN	denotes	truncated
T13046	14725-14727	NN	denotes	mr
T13047	14727-14728	HYPH	denotes	-
T13040	14728-14729	NN	denotes	s
T13048	14730-14731	-LRB-	denotes	(
T13050	14731-14735	NN	denotes	Fig.
T13049	14736-14738	NN	denotes	5A
T13051	14738-14739	-RRB-	denotes	)
T13052	14739-14740	.	denotes	.
T13053	14740-14827	sentence	denotes	As a negative control, we constructed Flag-tagged Sonic hedgehog (Shh) (lane 2 and 7).
T13054	14741-14743	IN	denotes	As
T13056	14744-14745	DT	denotes	a
T13058	14746-14754	JJ	denotes	negative
T13057	14755-14762	NN	denotes	control
T13059	14762-14764	,	denotes	,
T13060	14764-14766	PRP	denotes	we
T13055	14767-14778	VBD	denotes	constructed
T13061	14779-14783	NN	denotes	Flag
T13063	14783-14784	HYPH	denotes	-
T13062	14784-14790	VBN	denotes	tagged
T13065	14791-14796	JJ	denotes	Sonic
T13064	14797-14805	NN	denotes	hedgehog
T13066	14806-14807	-LRB-	denotes	(
T13067	14807-14810	NN	denotes	Shh
T13068	14810-14811	-RRB-	denotes	)
T13069	14812-14813	-LRB-	denotes	(
T13071	14813-14817	NN	denotes	lane
T13070	14818-14819	CD	denotes	2
T13072	14820-14823	CC	denotes	and
T13073	14824-14825	CD	denotes	7
T13074	14825-14826	-RRB-	denotes	)
T13075	14826-14827	.	denotes	.
T13076	14827-15118	sentence	denotes	In accordance with the result of the yeast two-hybrid GAL4 assay, HA-tagged full-length mr-s (full-HA) was co-immunoprecipitated with Flag-tagged full-length mr-s (Flag-mrs) and the Flag-tagged C-terminus portion containing the SAM domain (Flag-SAM), respectively (Fig 5B, lane3 and lane5).
T13077	14828-14830	IN	denotes	In
T13079	14831-14841	NN	denotes	accordance
T13080	14842-14846	IN	denotes	with
T13081	14847-14850	DT	denotes	the
T13082	14851-14857	NN	denotes	result
T13083	14858-14860	IN	denotes	of
T13084	14861-14864	DT	denotes	the
T13086	14865-14870	NN	denotes	yeast
T13087	14871-14874	CD	denotes	two
T13089	14874-14875	HYPH	denotes	-
T13088	14875-14881	NN	denotes	hybrid
T13090	14882-14886	NN	denotes	GAL4
T13085	14887-14892	NN	denotes	assay
T13091	14892-14894	,	denotes	,
T13092	14894-14896	NN	denotes	HA
T13094	14896-14897	HYPH	denotes	-
T13093	14897-14903	VBN	denotes	tagged
T13096	14904-14908	JJ	denotes	full
T13098	14908-14909	HYPH	denotes	-
T13097	14909-14915	NN	denotes	length
T13099	14916-14918	NN	denotes	mr
T13100	14918-14919	HYPH	denotes	-
T13095	14919-14920	NN	denotes	s
T13101	14921-14922	-LRB-	denotes	(
T13102	14922-14926	JJ	denotes	full
T13104	14926-14927	HYPH	denotes	-
T13103	14927-14929	NN	denotes	HA
T13105	14929-14930	-RRB-	denotes	)
T13106	14931-14934	VBD	denotes	was
T13078	14935-14956	VBN	denotes	co-immunoprecipitated
T13107	14957-14961	IN	denotes	with
T13108	14962-14966	NN	denotes	Flag
T13110	14966-14967	HYPH	denotes	-
T13109	14967-14973	VBN	denotes	tagged
T13112	14974-14978	JJ	denotes	full
T13114	14978-14979	HYPH	denotes	-
T13113	14979-14985	NN	denotes	length
T13115	14986-14988	NN	denotes	mr
T13116	14988-14989	HYPH	denotes	-
T13111	14989-14990	NN	denotes	s
T13117	14991-14992	-LRB-	denotes	(
T13118	14992-14996	NN	denotes	Flag
T13120	14996-14997	HYPH	denotes	-
T13119	14997-15000	NN	denotes	mrs
T13121	15000-15001	-RRB-	denotes	)
T13122	15002-15005	CC	denotes	and
T13123	15006-15009	DT	denotes	the
T13125	15010-15014	NN	denotes	Flag
T13127	15014-15015	HYPH	denotes	-
T13126	15015-15021	VBN	denotes	tagged
T13128	15022-15023	NN	denotes	C
T13130	15023-15024	HYPH	denotes	-
T13129	15024-15032	NN	denotes	terminus
T13124	15033-15040	NN	denotes	portion
T13131	15041-15051	VBG	denotes	containing
T13132	15052-15055	DT	denotes	the
T13134	15056-15059	NN	denotes	SAM
T13133	15060-15066	NN	denotes	domain
T13135	15067-15068	-LRB-	denotes	(
T13136	15068-15072	NN	denotes	Flag
T13138	15072-15073	HYPH	denotes	-
T13137	15073-15076	NN	denotes	SAM
T13139	15076-15077	-RRB-	denotes	)
T13140	15077-15079	,	denotes	,
T13141	15079-15091	RB	denotes	respectively
T13142	15092-15093	-LRB-	denotes	(
T13144	15093-15096	NN	denotes	Fig
T13145	15097-15099	NN	denotes	5B
T13146	15099-15101	,	denotes	,
T13143	15101-15106	NN	denotes	lane3
T13147	15107-15110	CC	denotes	and
T13148	15111-15116	NN	denotes	lane5
T13149	15116-15117	-RRB-	denotes	)
T13150	15117-15118	.	denotes	.
T13151	15118-15270	sentence	denotes	We also found a weak co-immunoprecipitation band in co-transfection of full-HA and Flag-tagged N-terminus portion of mr-s (Flag-ΔSAM, Fig. 5B, lane 4).
T13152	15119-15121	PRP	denotes	We
T13154	15122-15126	RB	denotes	also
T13153	15127-15132	VBD	denotes	found
T13155	15133-15134	DT	denotes	a
T13157	15135-15139	JJ	denotes	weak
T13158	15140-15162	NN	denotes	co-immunoprecipitation
T13156	15163-15167	NN	denotes	band
T13159	15168-15170	IN	denotes	in
T13160	15171-15186	NN	denotes	co-transfection
T13161	15187-15189	IN	denotes	of
T13162	15190-15194	JJ	denotes	full
T13164	15194-15195	HYPH	denotes	-
T13163	15195-15197	NN	denotes	HA
T13165	15198-15201	CC	denotes	and
T13166	15202-15206	NN	denotes	Flag
T13168	15206-15207	HYPH	denotes	-
T13167	15207-15213	VBN	denotes	tagged
T13170	15214-15215	NN	denotes	N
T13172	15215-15216	HYPH	denotes	-
T13171	15216-15224	NN	denotes	terminus
T13169	15225-15232	NN	denotes	portion
T13173	15233-15235	IN	denotes	of
T13174	15236-15238	NN	denotes	mr
T13176	15238-15239	HYPH	denotes	-
T13175	15239-15240	NN	denotes	s
T13177	15241-15242	-LRB-	denotes	(
T13178	15242-15246	NN	denotes	Flag
T13180	15246-15247	HYPH	denotes	-
T13179	15247-15251	NN	denotes	ΔSAM
T13181	15251-15253	,	denotes	,
T13183	15253-15257	NN	denotes	Fig.
T13184	15258-15260	NN	denotes	5B
T13185	15260-15262	,	denotes	,
T13182	15262-15266	NN	denotes	lane
T13186	15267-15268	CD	denotes	4
T13187	15268-15269	-RRB-	denotes	)
T13188	15269-15270	.	denotes	.
T13189	15270-15507	sentence	denotes	When ΔSAM-HA and Flag-tagged deletion mutants were co-transfected, ΔSAM-HA was co-immunoprecipitated with Flag-mrs and Flag-ΔSAM (Fig. 5B, lane 8 and lane 9), while ΔSAM-HA was not co-immunoprecipitated with Flag-SAM (Fig. 5B, lane 10).
T13190	15271-15275	WRB	denotes	When
T13192	15276-15280	NN	denotes	ΔSAM
T13194	15280-15281	HYPH	denotes	-
T13193	15281-15283	NN	denotes	HA
T13195	15284-15287	CC	denotes	and
T13196	15288-15292	NN	denotes	Flag
T13198	15292-15293	HYPH	denotes	-
T13197	15293-15299	VBN	denotes	tagged
T13200	15300-15308	NN	denotes	deletion
T13199	15309-15316	NNS	denotes	mutants
T13201	15317-15321	VBD	denotes	were
T13191	15322-15336	VBN	denotes	co-transfected
T13203	15336-15338	,	denotes	,
T13204	15338-15342	NN	denotes	ΔSAM
T13206	15342-15343	HYPH	denotes	-
T13205	15343-15345	NN	denotes	HA
T13207	15346-15349	VBD	denotes	was
T13202	15350-15371	VBN	denotes	co-immunoprecipitated
T13208	15372-15376	IN	denotes	with
T13209	15377-15381	NN	denotes	Flag
T13211	15381-15382	HYPH	denotes	-
T13210	15382-15385	NN	denotes	mrs
T13212	15386-15389	CC	denotes	and
T13213	15390-15394	NN	denotes	Flag
T13215	15394-15395	HYPH	denotes	-
T13214	15395-15399	NN	denotes	ΔSAM
T13216	15400-15401	-LRB-	denotes	(
T13218	15401-15405	NN	denotes	Fig.
T13217	15406-15408	NN	denotes	5B
T13219	15408-15410	,	denotes	,
T13220	15410-15414	NN	denotes	lane
T13221	15415-15416	CD	denotes	8
T13222	15417-15420	CC	denotes	and
T13223	15421-15425	NN	denotes	lane
T13224	15426-15427	CD	denotes	9
T13225	15427-15428	-RRB-	denotes	)
T13226	15428-15430	,	denotes	,
T13227	15430-15435	IN	denotes	while
T13229	15436-15440	NN	denotes	ΔSAM
T13231	15440-15441	HYPH	denotes	-
T13230	15441-15443	NN	denotes	HA
T13232	15444-15447	VBD	denotes	was
T13233	15448-15451	RB	denotes	not
T13228	15452-15473	VBN	denotes	co-immunoprecipitated
T13234	15474-15478	IN	denotes	with
T13235	15479-15483	NN	denotes	Flag
T13237	15483-15484	HYPH	denotes	-
T13236	15484-15487	NN	denotes	SAM
T13238	15488-15489	-LRB-	denotes	(
T13240	15489-15493	NN	denotes	Fig.
T13239	15494-15496	NN	denotes	5B
T13241	15496-15498	,	denotes	,
T13242	15498-15502	NN	denotes	lane
T13243	15503-15505	CD	denotes	10
T13244	15505-15506	-RRB-	denotes	)
T13245	15506-15507	.	denotes	.
T13246	15507-16433	sentence	denotes	Figure 5 The mr-s protein can self-associate in mammalian cells. (A) Schematic drawing of the constructs used for immunoprecipitation assay. HA-tagged or Flag-tagged full-length (amino acids 1 to 542), ΔSAM (amino acids 1 to 400) and SAM (amino acids 401 to 542) regions were inserted into pcDNA3 vector, respectively. (B) The constructs indicated above were transfected into HEK293T cells. Each lane was co-immunoprecipitated by anti-Flag antibody and detected by anti-HA antibody. Input protein lysates are shown in the lower panels. (C) Flag-tagged two site-directed mr-s mutants, Flag-W404A and Flag-G453A were generated and co-transfected with full-HA. Each lane was co-immunoprecipitated by anti-HA antibody and detected by anti-Flag antibody. To investigate whether the mr-s protein self-associates mainly through the SAM domain, two site-directed mutations were generated in the SAM domain of mr-s (Fig. 1B, arrows).
T31407	15518-15521	DT	denotes	The
T31409	15522-15524	NN	denotes	mr
T31411	15524-15525	HYPH	denotes	-
T31410	15525-15526	NN	denotes	s
T31408	15527-15534	NN	denotes	protein
T31413	15535-15538	MD	denotes	can
T31414	15539-15543	NN	denotes	self
T31415	15543-15544	HYPH	denotes	-
T31412	15544-15553	VB	denotes	associate
T31416	15554-15556	IN	denotes	in
T31417	15557-15566	JJ	denotes	mammalian
T31418	15567-15572	NNS	denotes	cells
T31419	15572-15573	.	denotes	.
T31420	15573-15649	sentence	denotes	(A) Schematic drawing of the constructs used for immunoprecipitation assay.
T31421	15574-15575	-LRB-	denotes	(
T31422	15575-15576	LS	denotes	A
T31424	15576-15577	-RRB-	denotes	)
T31425	15578-15587	JJ	denotes	Schematic
T31423	15588-15595	NN	denotes	drawing
T31426	15596-15598	IN	denotes	of
T31427	15599-15602	DT	denotes	the
T31428	15603-15613	NNS	denotes	constructs
T31429	15614-15618	VBN	denotes	used
T31430	15619-15622	IN	denotes	for
T31431	15623-15642	NN	denotes	immunoprecipitation
T31432	15643-15648	NN	denotes	assay
T31433	15648-15649	.	denotes	.
T31434	15649-15827	sentence	denotes	HA-tagged or Flag-tagged full-length (amino acids 1 to 542), ΔSAM (amino acids 1 to 400) and SAM (amino acids 401 to 542) regions were inserted into pcDNA3 vector, respectively.
T31435	15650-15652	NN	denotes	HA
T31437	15652-15653	HYPH	denotes	-
T31436	15653-15659	VBN	denotes	tagged
T31439	15660-15662	CC	denotes	or
T31440	15663-15667	NN	denotes	Flag
T31442	15667-15668	HYPH	denotes	-
T31441	15668-15674	VBN	denotes	tagged
T31443	15675-15679	JJ	denotes	full
T31445	15679-15680	HYPH	denotes	-
T31444	15680-15686	NN	denotes	length
T31446	15687-15688	-LRB-	denotes	(
T31448	15688-15693	NN	denotes	amino
T31449	15694-15699	NNS	denotes	acids
T31447	15700-15701	CD	denotes	1
T31450	15702-15704	IN	denotes	to
T31451	15705-15708	CD	denotes	542
T31452	15708-15709	-RRB-	denotes	)
T31453	15709-15711	,	denotes	,
T31454	15711-15715	NN	denotes	ΔSAM
T31455	15716-15717	-LRB-	denotes	(
T31457	15717-15722	NN	denotes	amino
T31458	15723-15728	NNS	denotes	acids
T31456	15729-15730	CD	denotes	1
T31459	15731-15733	IN	denotes	to
T31460	15734-15737	CD	denotes	400
T31461	15737-15738	-RRB-	denotes	)
T31462	15739-15742	CC	denotes	and
T31463	15743-15746	NN	denotes	SAM
T31464	15747-15748	-LRB-	denotes	(
T31466	15748-15753	NN	denotes	amino
T31467	15754-15759	NNS	denotes	acids
T31465	15760-15763	CD	denotes	401
T31468	15764-15766	IN	denotes	to
T31469	15767-15770	CD	denotes	542
T31470	15770-15771	-RRB-	denotes	)
T31438	15772-15779	NNS	denotes	regions
T31472	15780-15784	VBD	denotes	were
T31471	15785-15793	VBN	denotes	inserted
T31473	15794-15798	IN	denotes	into
T31474	15799-15805	NN	denotes	pcDNA3
T31475	15806-15812	NN	denotes	vector
T31476	15812-15814	,	denotes	,
T31477	15814-15826	RB	denotes	respectively
T31478	15826-15827	.	denotes	.
T31479	15827-15899	sentence	denotes	(B) The constructs indicated above were transfected into HEK293T cells.
T31480	15828-15829	-LRB-	denotes	(
T31481	15829-15830	LS	denotes	B
T31483	15830-15831	-RRB-	denotes	)
T31484	15832-15835	DT	denotes	The
T31485	15836-15846	NNS	denotes	constructs
T31486	15847-15856	VBN	denotes	indicated
T31487	15857-15862	RB	denotes	above
T31488	15863-15867	VBD	denotes	were
T31482	15868-15879	VBN	denotes	transfected
T31489	15880-15884	IN	denotes	into
T31490	15885-15892	NN	denotes	HEK293T
T31491	15893-15898	NNS	denotes	cells
T31492	15898-15899	.	denotes	.
T31493	15899-15991	sentence	denotes	Each lane was co-immunoprecipitated by anti-Flag antibody and detected by anti-HA antibody.
T31494	15900-15904	DT	denotes	Each
T31495	15905-15909	NN	denotes	lane
T31497	15910-15913	VBD	denotes	was
T31496	15914-15935	VBN	denotes	co-immunoprecipitated
T31498	15936-15938	IN	denotes	by
T31499	15939-15948	JJ	denotes	anti-Flag
T31500	15949-15957	NN	denotes	antibody
T31501	15958-15961	CC	denotes	and
T31502	15962-15970	VBN	denotes	detected
T31503	15971-15973	IN	denotes	by
T31504	15974-15981	JJ	denotes	anti-HA
T31505	15982-15990	NN	denotes	antibody
T31506	15990-15991	.	denotes	.
T31507	15991-16044	sentence	denotes	Input protein lysates are shown in the lower panels.
T31508	15992-15997	NN	denotes	Input
T31510	15998-16005	NN	denotes	protein
T31509	16006-16013	NNS	denotes	lysates
T31512	16014-16017	VBP	denotes	are
T31511	16018-16023	VBN	denotes	shown
T31513	16024-16026	IN	denotes	in
T31514	16027-16030	DT	denotes	the
T31516	16031-16036	JJ	denotes	lower
T31515	16037-16043	NNS	denotes	panels
T31517	16043-16044	.	denotes	.
T31518	16044-16166	sentence	denotes	(C) Flag-tagged two site-directed mr-s mutants, Flag-W404A and Flag-G453A were generated and co-transfected with full-HA.
T31519	16045-16046	-LRB-	denotes	(
T31520	16046-16047	LS	denotes	C
T31522	16047-16048	-RRB-	denotes	)
T31523	16049-16053	NN	denotes	Flag
T31525	16053-16054	HYPH	denotes	-
T31524	16054-16060	VBN	denotes	tagged
T31527	16061-16064	CD	denotes	two
T31528	16065-16069	NN	denotes	site
T31530	16069-16070	HYPH	denotes	-
T31529	16070-16078	VBN	denotes	directed
T31531	16079-16081	NN	denotes	mr
T31533	16081-16082	HYPH	denotes	-
T31532	16082-16083	NN	denotes	s
T31526	16084-16091	NNS	denotes	mutants
T31534	16091-16093	,	denotes	,
T31535	16093-16097	NN	denotes	Flag
T31537	16097-16098	HYPH	denotes	-
T31536	16098-16103	NN	denotes	W404A
T31538	16104-16107	CC	denotes	and
T31539	16108-16112	NN	denotes	Flag
T31541	16112-16113	HYPH	denotes	-
T31540	16113-16118	NN	denotes	G453A
T31542	16119-16123	VBD	denotes	were
T31521	16124-16133	VBN	denotes	generated
T31543	16134-16137	CC	denotes	and
T31544	16138-16152	VBN	denotes	co-transfected
T31545	16153-16157	IN	denotes	with
T31546	16158-16162	JJ	denotes	full
T31548	16162-16163	HYPH	denotes	-
T31547	16163-16165	NN	denotes	HA
T31549	16165-16166	.	denotes	.
T31550	16166-16258	sentence	denotes	Each lane was co-immunoprecipitated by anti-HA antibody and detected by anti-Flag antibody.
T31551	16167-16171	DT	denotes	Each
T31552	16172-16176	NN	denotes	lane
T31554	16177-16180	VBD	denotes	was
T31553	16181-16202	VBN	denotes	co-immunoprecipitated
T31555	16203-16205	IN	denotes	by
T31556	16206-16213	JJ	denotes	anti-HA
T31557	16214-16222	NN	denotes	antibody
T31558	16223-16226	CC	denotes	and
T31559	16227-16235	VBN	denotes	detected
T31560	16236-16238	IN	denotes	by
T31561	16239-16248	JJ	denotes	anti-Flag
T31562	16249-16257	NN	denotes	antibody
T31563	16257-16258	.	denotes	.
T13247	16259-16261	TO	denotes	To
T13248	16262-16273	VB	denotes	investigate
T13250	16274-16281	IN	denotes	whether
T13252	16282-16285	DT	denotes	the
T13254	16286-16288	NN	denotes	mr
T13256	16288-16289	HYPH	denotes	-
T13255	16289-16290	NN	denotes	s
T13253	16291-16298	NN	denotes	protein
T13257	16299-16303	NN	denotes	self
T13258	16303-16304	HYPH	denotes	-
T13251	16304-16314	VBZ	denotes	associates
T13259	16315-16321	RB	denotes	mainly
T13260	16322-16329	IN	denotes	through
T13261	16330-16333	DT	denotes	the
T13263	16334-16337	NN	denotes	SAM
T13262	16338-16344	NN	denotes	domain
T13264	16344-16346	,	denotes	,
T13265	16346-16349	CD	denotes	two
T13267	16350-16354	NN	denotes	site
T13269	16354-16355	HYPH	denotes	-
T13268	16355-16363	VBN	denotes	directed
T13266	16364-16373	NNS	denotes	mutations
T13270	16374-16378	VBD	denotes	were
T13249	16379-16388	VBN	denotes	generated
T13271	16389-16391	IN	denotes	in
T13272	16392-16395	DT	denotes	the
T13274	16396-16399	NN	denotes	SAM
T13273	16400-16406	NN	denotes	domain
T13275	16407-16409	IN	denotes	of
T13276	16410-16412	NN	denotes	mr
T13278	16412-16413	HYPH	denotes	-
T13277	16413-16414	NN	denotes	s
T13279	16415-16416	-LRB-	denotes	(
T13281	16416-16420	NN	denotes	Fig.
T13282	16421-16423	NN	denotes	1B
T13283	16423-16425	,	denotes	,
T13280	16425-16431	NNS	denotes	arrows
T13284	16431-16432	-RRB-	denotes	)
T13285	16432-16433	.	denotes	.
T13286	16433-16691	sentence	denotes	These mutations alter residues that are conserved in the SAM domain of ph and previous report indicates that these mutations of ph-SAM cause significant reduction in binding activity to the other SAM domain-containing protein, Sex comb on midleg (Scm) (41).
T13287	16434-16439	DT	denotes	These
T13288	16440-16449	NNS	denotes	mutations
T13289	16450-16455	VBP	denotes	alter
T13290	16456-16464	NNS	denotes	residues
T13291	16465-16469	WDT	denotes	that
T13293	16470-16473	VBP	denotes	are
T13292	16474-16483	VBN	denotes	conserved
T13294	16484-16486	IN	denotes	in
T13295	16487-16490	DT	denotes	the
T13297	16491-16494	NN	denotes	SAM
T13296	16495-16501	NN	denotes	domain
T13298	16502-16504	IN	denotes	of
T13299	16505-16507	NN	denotes	ph
T13300	16508-16511	CC	denotes	and
T13301	16512-16520	JJ	denotes	previous
T13302	16521-16527	NN	denotes	report
T13303	16528-16537	VBZ	denotes	indicates
T13304	16538-16542	IN	denotes	that
T13306	16543-16548	DT	denotes	these
T13307	16549-16558	NNS	denotes	mutations
T13308	16559-16561	IN	denotes	of
T13309	16562-16564	NN	denotes	ph
T13311	16564-16565	HYPH	denotes	-
T13310	16565-16568	NN	denotes	SAM
T13305	16569-16574	VBP	denotes	cause
T13312	16575-16586	JJ	denotes	significant
T13313	16587-16596	NN	denotes	reduction
T13314	16597-16599	IN	denotes	in
T13315	16600-16607	NN	denotes	binding
T13316	16608-16616	NN	denotes	activity
T13317	16617-16619	IN	denotes	to
T13318	16620-16623	DT	denotes	the
T13320	16624-16629	JJ	denotes	other
T13321	16630-16633	NN	denotes	SAM
T13322	16634-16640	NN	denotes	domain
T13324	16640-16641	HYPH	denotes	-
T13323	16641-16651	VBG	denotes	containing
T13319	16652-16659	NN	denotes	protein
T13325	16659-16661	,	denotes	,
T13326	16661-16664	NN	denotes	Sex
T13327	16665-16669	NN	denotes	comb
T13328	16670-16672	IN	denotes	on
T13329	16673-16679	NN	denotes	midleg
T13330	16680-16681	-LRB-	denotes	(
T13331	16681-16684	NN	denotes	Scm
T13332	16684-16685	-RRB-	denotes	)
T13333	16686-16687	-LRB-	denotes	(
T13334	16687-16689	CD	denotes	41
T13335	16689-16690	-RRB-	denotes	)
T13336	16690-16691	.	denotes	.
T13337	16691-16888	sentence	denotes	Based on this result, we introduced two types of site-directed mutations, which correspond to the mutations introduced in ph protein, into Flag-tagged full-length mr-s (Flag-W404A and Flag-G453A).
T13338	16692-16697	VBN	denotes	Based
T13340	16698-16700	IN	denotes	on
T13341	16701-16705	DT	denotes	this
T13342	16706-16712	NN	denotes	result
T13343	16712-16714	,	denotes	,
T13344	16714-16716	PRP	denotes	we
T13339	16717-16727	VBD	denotes	introduced
T13345	16728-16731	CD	denotes	two
T13346	16732-16737	NNS	denotes	types
T13347	16738-16740	IN	denotes	of
T13348	16741-16745	NN	denotes	site
T13350	16745-16746	HYPH	denotes	-
T13349	16746-16754	VBN	denotes	directed
T13351	16755-16764	NNS	denotes	mutations
T13352	16764-16766	,	denotes	,
T13353	16766-16771	WDT	denotes	which
T13354	16772-16782	VBP	denotes	correspond
T13355	16783-16785	IN	denotes	to
T13356	16786-16789	DT	denotes	the
T13357	16790-16799	NNS	denotes	mutations
T13358	16800-16810	VBN	denotes	introduced
T13359	16811-16813	IN	denotes	in
T13360	16814-16816	NN	denotes	ph
T13361	16817-16824	NN	denotes	protein
T13362	16824-16826	,	denotes	,
T13363	16826-16830	IN	denotes	into
T13364	16831-16835	NN	denotes	Flag
T13366	16835-16836	HYPH	denotes	-
T13365	16836-16842	VBN	denotes	tagged
T13368	16843-16847	JJ	denotes	full
T13370	16847-16848	HYPH	denotes	-
T13369	16848-16854	NN	denotes	length
T13371	16855-16857	NN	denotes	mr
T13372	16857-16858	HYPH	denotes	-
T13367	16858-16859	NN	denotes	s
T13373	16860-16861	-LRB-	denotes	(
T13374	16861-16865	NN	denotes	Flag
T13376	16865-16866	HYPH	denotes	-
T13375	16866-16871	NN	denotes	W404A
T13377	16872-16875	CC	denotes	and
T13378	16876-16880	NN	denotes	Flag
T13380	16880-16881	HYPH	denotes	-
T13379	16881-16886	NN	denotes	G453A
T13381	16886-16887	-RRB-	denotes	)
T13382	16887-16888	.	denotes	.
T13383	16888-17046	sentence	denotes	We found that Flag-W404A binding activity was significantly reduced and Flag-G453A binding activity was also slightly reduced compared to Flag-mrs (Fig. 5C).
T13384	16889-16891	PRP	denotes	We
T13385	16892-16897	VBD	denotes	found
T13386	16898-16902	IN	denotes	that
T13388	16903-16907	NN	denotes	Flag
T13390	16907-16908	HYPH	denotes	-
T13389	16908-16913	NN	denotes	W404A
T13391	16914-16921	NN	denotes	binding
T13392	16922-16930	NN	denotes	activity
T13393	16931-16934	VBD	denotes	was
T13394	16935-16948	RB	denotes	significantly
T13387	16949-16956	VBN	denotes	reduced
T13395	16957-16960	CC	denotes	and
T13396	16961-16965	NN	denotes	Flag
T13398	16965-16966	HYPH	denotes	-
T13397	16966-16971	NN	denotes	G453A
T13399	16972-16979	NN	denotes	binding
T13400	16980-16988	NN	denotes	activity
T13402	16989-16992	VBD	denotes	was
T13403	16993-16997	RB	denotes	also
T13404	16998-17006	RB	denotes	slightly
T13401	17007-17014	VBN	denotes	reduced
T13405	17015-17023	VBN	denotes	compared
T13406	17024-17026	IN	denotes	to
T13407	17027-17031	NN	denotes	Flag
T13409	17031-17032	HYPH	denotes	-
T13408	17032-17035	NN	denotes	mrs
T13410	17036-17037	-LRB-	denotes	(
T13412	17037-17041	NN	denotes	Fig.
T13411	17042-17044	NN	denotes	5C
T13413	17044-17045	-RRB-	denotes	)
T13414	17045-17046	.	denotes	.
T13415	17046-17245	sentence	denotes	These results, together with yeast two-hybrid GAL4 assay, indicate that the mr-s protein self-associates strongly through its SAM domain and weakly through the N-terminus portion lacking SAM domain.
T13416	17047-17052	DT	denotes	These
T13417	17053-17060	NNS	denotes	results
T13419	17060-17062	,	denotes	,
T13420	17062-17070	RB	denotes	together
T13421	17071-17075	IN	denotes	with
T13422	17076-17081	NN	denotes	yeast
T13424	17082-17085	CD	denotes	two
T13426	17085-17086	HYPH	denotes	-
T13425	17086-17092	NN	denotes	hybrid
T13427	17093-17097	NN	denotes	GAL4
T13423	17098-17103	NN	denotes	assay
T13428	17103-17105	,	denotes	,
T13418	17105-17113	VBP	denotes	indicate
T13429	17114-17118	IN	denotes	that
T13431	17119-17122	DT	denotes	the
T13433	17123-17125	NN	denotes	mr
T13435	17125-17126	HYPH	denotes	-
T13434	17126-17127	NN	denotes	s
T13432	17128-17135	NN	denotes	protein
T13436	17136-17140	NN	denotes	self
T13437	17140-17141	HYPH	denotes	-
T13430	17141-17151	VBZ	denotes	associates
T13438	17152-17160	RB	denotes	strongly
T13439	17161-17168	IN	denotes	through
T13440	17169-17172	PRP$	denotes	its
T13442	17173-17176	NN	denotes	SAM
T13441	17177-17183	NN	denotes	domain
T13443	17184-17187	CC	denotes	and
T13444	17188-17194	RB	denotes	weakly
T13445	17195-17202	IN	denotes	through
T13446	17203-17206	DT	denotes	the
T13448	17207-17208	NN	denotes	N
T13450	17208-17209	HYPH	denotes	-
T13449	17209-17217	NN	denotes	terminus
T13447	17218-17225	NN	denotes	portion
T13451	17226-17233	VBG	denotes	lacking
T13452	17234-17237	NN	denotes	SAM
T13453	17238-17244	NN	denotes	domain
T13454	17244-17245	.	denotes	.
T13863	17247-17250	DT	denotes	The
T13865	17251-17262	JJ	denotes	subcellular
T13864	17263-17275	NN	denotes	localization
T13866	17276-17278	IN	denotes	of
T13867	17279-17281	NN	denotes	mr
T13869	17281-17282	HYPH	denotes	-
T13868	17282-17283	NN	denotes	s
T13870	17284-17291	NN	denotes	protein
T13871	17292-17294	IN	denotes	in
T13872	17295-17304	JJ	denotes	mammalian
T13873	17305-17310	NNS	denotes	cells
T13874	17310-17468	sentence	denotes	The putative nuclear localization signal at the N-terminus of the mr-s protein (Fig. 1A, dashed box) suggests that the mr-s protein localizes in the nucleus.
T13875	17311-17314	DT	denotes	The
T13877	17315-17323	JJ	denotes	putative
T13878	17324-17331	JJ	denotes	nuclear
T13879	17332-17344	NN	denotes	localization
T13876	17345-17351	NN	denotes	signal
T13881	17352-17354	IN	denotes	at
T13882	17355-17358	DT	denotes	the
T13884	17359-17360	NN	denotes	N
T13885	17360-17361	HYPH	denotes	-
T13883	17361-17369	NN	denotes	terminus
T13886	17370-17372	IN	denotes	of
T13887	17373-17376	DT	denotes	the
T13889	17377-17379	NN	denotes	mr
T13891	17379-17380	HYPH	denotes	-
T13890	17380-17381	NN	denotes	s
T13888	17382-17389	NN	denotes	protein
T13892	17390-17391	-LRB-	denotes	(
T13894	17391-17395	NN	denotes	Fig.
T13895	17396-17398	NN	denotes	1A
T13896	17398-17400	,	denotes	,
T13897	17400-17406	JJ	denotes	dashed
T13893	17407-17410	NN	denotes	box
T13898	17410-17411	-RRB-	denotes	)
T13880	17412-17420	VBZ	denotes	suggests
T13899	17421-17425	IN	denotes	that
T13901	17426-17429	DT	denotes	the
T13903	17430-17432	NN	denotes	mr
T13905	17432-17433	HYPH	denotes	-
T13904	17433-17434	NN	denotes	s
T13902	17435-17442	NN	denotes	protein
T13900	17443-17452	VBZ	denotes	localizes
T13906	17453-17455	IN	denotes	in
T13907	17456-17459	DT	denotes	the
T13908	17460-17467	NN	denotes	nucleus
T13909	17467-17468	.	denotes	.
T13910	17468-17610	sentence	denotes	To determine the subcellular localization of mr-s in mammalian cells, we introduced an HA-tagged full-length mr-s plasmid into HEK293T cells.
T13911	17469-17471	TO	denotes	To
T13912	17472-17481	VB	denotes	determine
T13914	17482-17485	DT	denotes	the
T13916	17486-17497	JJ	denotes	subcellular
T13915	17498-17510	NN	denotes	localization
T13917	17511-17513	IN	denotes	of
T13918	17514-17516	NN	denotes	mr
T13920	17516-17517	HYPH	denotes	-
T13919	17517-17518	NN	denotes	s
T13921	17519-17521	IN	denotes	in
T13922	17522-17531	JJ	denotes	mammalian
T13923	17532-17537	NNS	denotes	cells
T13924	17537-17539	,	denotes	,
T13925	17539-17541	PRP	denotes	we
T13913	17542-17552	VBD	denotes	introduced
T13926	17553-17555	DT	denotes	an
T13928	17556-17558	NN	denotes	HA
T13930	17558-17559	HYPH	denotes	-
T13929	17559-17565	VBN	denotes	tagged
T13931	17566-17570	JJ	denotes	full
T13933	17570-17571	HYPH	denotes	-
T13932	17571-17577	NN	denotes	length
T13934	17578-17580	NN	denotes	mr
T13936	17580-17581	HYPH	denotes	-
T13935	17581-17582	NN	denotes	s
T13927	17583-17590	NN	denotes	plasmid
T13937	17591-17595	IN	denotes	into
T13938	17596-17603	NN	denotes	HEK293T
T13939	17604-17609	NNS	denotes	cells
T13940	17609-17610	.	denotes	.
T13941	17610-17722	sentence	denotes	Confocal microscopy showed that the mr-s protein localized mainly in the nucleus of HEK293T cells (Fig. 6A, B).
T13942	17611-17619	JJ	denotes	Confocal
T13943	17620-17630	NN	denotes	microscopy
T13944	17631-17637	VBD	denotes	showed
T13945	17638-17642	IN	denotes	that
T13947	17643-17646	DT	denotes	the
T13949	17647-17649	NN	denotes	mr
T13951	17649-17650	HYPH	denotes	-
T13950	17650-17651	NN	denotes	s
T13948	17652-17659	NN	denotes	protein
T13946	17660-17669	VBN	denotes	localized
T13952	17670-17676	RB	denotes	mainly
T13953	17677-17679	IN	denotes	in
T13954	17680-17683	DT	denotes	the
T13955	17684-17691	NN	denotes	nucleus
T13956	17692-17694	IN	denotes	of
T13957	17695-17702	NN	denotes	HEK293T
T13958	17703-17708	NNS	denotes	cells
T13959	17709-17710	-LRB-	denotes	(
T13961	17710-17714	NN	denotes	Fig.
T13962	17715-17717	NN	denotes	6A
T13963	17717-17719	,	denotes	,
T13960	17719-17720	NN	denotes	B
T13964	17720-17721	-RRB-	denotes	)
T13965	17721-17722	.	denotes	.
T13966	17722-17840	sentence	denotes	To confirm the precise localization of the mr-s protein, full-length mr-s was co-immunostained with DAPI (Fig. 6C–E).
T13967	17723-17725	TO	denotes	To
T13968	17726-17733	VB	denotes	confirm
T13970	17734-17737	DT	denotes	the
T13972	17738-17745	JJ	denotes	precise
T13971	17746-17758	NN	denotes	localization
T13973	17759-17761	IN	denotes	of
T13974	17762-17765	DT	denotes	the
T13976	17766-17768	NN	denotes	mr
T13978	17768-17769	HYPH	denotes	-
T13977	17769-17770	NN	denotes	s
T13975	17771-17778	NN	denotes	protein
T13979	17778-17780	,	denotes	,
T13980	17780-17784	JJ	denotes	full
T13982	17784-17785	HYPH	denotes	-
T13981	17785-17791	NN	denotes	length
T13984	17792-17794	NN	denotes	mr
T13985	17794-17795	HYPH	denotes	-
T13983	17795-17796	NN	denotes	s
T13986	17797-17800	VBD	denotes	was
T13969	17801-17817	VBN	denotes	co-immunostained
T13987	17818-17822	IN	denotes	with
T13988	17823-17827	NN	denotes	DAPI
T13989	17828-17829	-LRB-	denotes	(
T13991	17829-17833	NN	denotes	Fig.
T13990	17834-17836	NN	denotes	6C
T13992	17836-17837	SYM	denotes	–
T13993	17837-17838	NN	denotes	E
T13994	17838-17839	-RRB-	denotes	)
T13995	17839-17840	.	denotes	.
T13996	17840-18046	sentence	denotes	These data showed that the mr-s protein localizes preferentially to the nucleus in mammalian cells, supporting the idea that the mr-s protein is involved in transcriptional regulation as are ph and/or TEL.
T13997	17841-17846	DT	denotes	These
T13998	17847-17851	NNS	denotes	data
T13999	17852-17858	VBD	denotes	showed
T14000	17859-17863	IN	denotes	that
T14002	17864-17867	DT	denotes	the
T14004	17868-17870	NN	denotes	mr
T14006	17870-17871	HYPH	denotes	-
T14005	17871-17872	NN	denotes	s
T14003	17873-17880	NN	denotes	protein
T14001	17881-17890	VBZ	denotes	localizes
T14007	17891-17905	RB	denotes	preferentially
T14008	17906-17908	IN	denotes	to
T14009	17909-17912	DT	denotes	the
T14010	17913-17920	NN	denotes	nucleus
T14011	17921-17923	IN	denotes	in
T14012	17924-17933	JJ	denotes	mammalian
T14013	17934-17939	NNS	denotes	cells
T14014	17939-17941	,	denotes	,
T14015	17941-17951	VBG	denotes	supporting
T14016	17952-17955	DT	denotes	the
T14017	17956-17960	NN	denotes	idea
T14018	17961-17965	IN	denotes	that
T14020	17966-17969	DT	denotes	the
T14022	17970-17972	NN	denotes	mr
T14024	17972-17973	HYPH	denotes	-
T14023	17973-17974	NN	denotes	s
T14021	17975-17982	NN	denotes	protein
T14025	17983-17985	VBZ	denotes	is
T14019	17986-17994	VBN	denotes	involved
T14026	17995-17997	IN	denotes	in
T14027	17998-18013	JJ	denotes	transcriptional
T14028	18014-18024	NN	denotes	regulation
T14029	18025-18027	IN	denotes	as
T14030	18028-18031	VBP	denotes	are
T14031	18032-18034	NN	denotes	ph
T14032	18035-18038	CC	denotes	and
T14033	18038-18039	HYPH	denotes	/
T14034	18039-18041	CC	denotes	or
T14035	18042-18045	NN	denotes	TEL
T14036	18045-18046	.	denotes	.
T31729	18057-18068	JJ	denotes	Subcellular
T31730	18069-18081	NN	denotes	localization
T31731	18082-18084	IN	denotes	of
T31732	18085-18087	NN	denotes	mr
T31734	18087-18088	HYPH	denotes	-
T31733	18088-18089	NN	denotes	s
T31735	18090-18092	IN	denotes	in
T31736	18093-18102	JJ	denotes	mammalian
T31737	18103-18108	NNS	denotes	cells
T31738	18108-18109	.	denotes	.
T31739	18109-18214	sentence	denotes	(A, B) HA-tagged full-length mr-s was transfected into HEK293T (B) and detected by anti-HA antibody (A).
T31740	18110-18111	-LRB-	denotes	(
T31741	18111-18112	LS	denotes	A
T31743	18112-18114	,	denotes	,
T31744	18114-18115	LS	denotes	B
T31745	18115-18116	-RRB-	denotes	)
T31746	18117-18119	NN	denotes	HA
T31748	18119-18120	HYPH	denotes	-
T31747	18120-18126	VBN	denotes	tagged
T31750	18127-18131	JJ	denotes	full
T31752	18131-18132	HYPH	denotes	-
T31751	18132-18138	NN	denotes	length
T31753	18139-18141	NN	denotes	mr
T31754	18141-18142	HYPH	denotes	-
T31749	18142-18143	NN	denotes	s
T31755	18144-18147	VBD	denotes	was
T31742	18148-18159	VBN	denotes	transfected
T31756	18160-18164	IN	denotes	into
T31757	18165-18172	NN	denotes	HEK293T
T31758	18173-18174	-LRB-	denotes	(
T31759	18174-18175	NN	denotes	B
T31760	18175-18176	-RRB-	denotes	)
T31761	18177-18180	CC	denotes	and
T31762	18181-18189	VBN	denotes	detected
T31763	18190-18192	IN	denotes	by
T31764	18193-18200	JJ	denotes	anti-HA
T31765	18201-18209	NN	denotes	antibody
T31766	18210-18211	-LRB-	denotes	(
T31767	18211-18212	NN	denotes	A
T31768	18212-18213	-RRB-	denotes	)
T31769	18213-18214	.	denotes	.
T31770	18214-18232	sentence	denotes	Scale bar, 20 μm.
T31771	18215-18220	NN	denotes	Scale
T31772	18221-18224	NN	denotes	bar
T31773	18224-18226	,	denotes	,
T31774	18226-18228	CD	denotes	20
T31775	18229-18231	NN	denotes	μm
T31776	18231-18232	.	denotes	.
T31777	18232-18325	sentence	denotes	(C-E) HEK293T cells immunostained with anti-HA antibody (C), DAPI (D), and merged image (E).
T31778	18233-18234	-LRB-	denotes	(
T31779	18234-18235	LS	denotes	C
T31781	18235-18236	SYM	denotes	-
T31782	18236-18237	LS	denotes	E
T31783	18237-18238	-RRB-	denotes	)
T31784	18239-18246	NN	denotes	HEK293T
T31780	18247-18252	NNS	denotes	cells
T31785	18253-18266	VBN	denotes	immunostained
T31786	18267-18271	IN	denotes	with
T31787	18272-18279	JJ	denotes	anti-HA
T31788	18280-18288	NN	denotes	antibody
T31789	18289-18290	-LRB-	denotes	(
T31790	18290-18291	NN	denotes	C
T31791	18291-18292	-RRB-	denotes	)
T31792	18292-18294	,	denotes	,
T31793	18294-18298	NN	denotes	DAPI
T31794	18299-18300	-LRB-	denotes	(
T31795	18300-18301	NN	denotes	D
T31796	18301-18302	-RRB-	denotes	)
T31797	18302-18304	,	denotes	,
T31798	18304-18307	CC	denotes	and
T31799	18308-18314	VBN	denotes	merged
T31800	18315-18320	NN	denotes	image
T31801	18321-18322	-LRB-	denotes	(
T31802	18322-18323	NN	denotes	E
T31803	18323-18324	-RRB-	denotes	)
T31804	18324-18325	.	denotes	.
T31805	18325-18343	sentence	denotes	Scale bar, 50 μm.
T31806	18326-18331	NN	denotes	Scale
T31807	18332-18335	NN	denotes	bar
T31808	18335-18337	,	denotes	,
T31809	18337-18339	CD	denotes	50
T31810	18340-18342	NN	denotes	μm
T31811	18342-18343	.	denotes	.
T16058	18345-18348	DT	denotes	The
T16060	18349-18353	NN	denotes	GAL4
T16062	18353-18354	HYPH	denotes	-
T16063	18354-18356	NN	denotes	mr
T16065	18356-18357	HYPH	denotes	-
T16064	18357-18358	NN	denotes	s
T16061	18359-18365	NN	denotes	fusion
T16059	18366-18373	NN	denotes	protein
T16066	18374-18383	VBZ	denotes	functions
T16067	18384-18386	IN	denotes	as
T16068	18387-18402	JJ	denotes	transcriptional
T16069	18403-18412	NN	denotes	repressor
T16070	18412-18512	sentence	denotes	A member of PcG proteins, ph, does not contain an obvious sequence-specific DNA binding motif (16).
T16071	18413-18414	DT	denotes	A
T16072	18415-18421	NN	denotes	member
T16074	18422-18424	IN	denotes	of
T16075	18425-18428	NN	denotes	PcG
T16076	18429-18437	NN	denotes	proteins
T16077	18437-18439	,	denotes	,
T16078	18439-18441	NN	denotes	ph
T16079	18441-18443	,	denotes	,
T16080	18443-18447	VBZ	denotes	does
T16081	18448-18451	RB	denotes	not
T16073	18452-18459	VB	denotes	contain
T16082	18460-18462	DT	denotes	an
T16084	18463-18470	JJ	denotes	obvious
T16085	18471-18479	NN	denotes	sequence
T16087	18479-18480	HYPH	denotes	-
T16086	18480-18488	JJ	denotes	specific
T16088	18489-18492	NN	denotes	DNA
T16089	18493-18500	VBG	denotes	binding
T16083	18501-18506	NN	denotes	motif
T16090	18507-18508	-LRB-	denotes	(
T16091	18508-18510	CD	denotes	16
T16092	18510-18511	-RRB-	denotes	)
T16093	18511-18512	.	denotes	.
T16094	18512-18723	sentence	denotes	Ph functions as a transcriptional repressor through its polymerization and protein-protein interaction with other sequence-specific transcriptional repressors, which can form a higher order chromatin structure.
T16095	18513-18515	NN	denotes	Ph
T16096	18516-18525	VBZ	denotes	functions
T16097	18526-18528	IN	denotes	as
T16098	18529-18530	DT	denotes	a
T16100	18531-18546	JJ	denotes	transcriptional
T16099	18547-18556	NN	denotes	repressor
T16101	18557-18564	IN	denotes	through
T16102	18565-18568	PRP$	denotes	its
T16103	18569-18583	NN	denotes	polymerization
T16104	18584-18587	CC	denotes	and
T16105	18588-18595	NN	denotes	protein
T16107	18595-18596	HYPH	denotes	-
T16106	18596-18603	NN	denotes	protein
T16108	18604-18615	NN	denotes	interaction
T16109	18616-18620	IN	denotes	with
T16110	18621-18626	JJ	denotes	other
T16112	18627-18635	NN	denotes	sequence
T16114	18635-18636	HYPH	denotes	-
T16113	18636-18644	JJ	denotes	specific
T16115	18645-18660	JJ	denotes	transcriptional
T16111	18661-18671	NNS	denotes	repressors
T16116	18671-18673	,	denotes	,
T16117	18673-18678	WDT	denotes	which
T16119	18679-18682	MD	denotes	can
T16118	18683-18687	VB	denotes	form
T16120	18688-18689	DT	denotes	a
T16122	18690-18696	JJR	denotes	higher
T16123	18697-18702	NN	denotes	order
T16124	18703-18712	NN	denotes	chromatin
T16121	18713-18722	NN	denotes	structure
T16125	18722-18723	.	denotes	.
T16126	18723-18790	sentence	denotes	The mr-s protein also does not have an obvious DNA-binding domain.
T16127	18724-18727	DT	denotes	The
T16129	18728-18730	NN	denotes	mr
T16131	18730-18731	HYPH	denotes	-
T16130	18731-18732	NN	denotes	s
T16128	18733-18740	NN	denotes	protein
T16133	18741-18745	RB	denotes	also
T16134	18746-18750	VBZ	denotes	does
T16135	18751-18754	RB	denotes	not
T16132	18755-18759	VB	denotes	have
T16136	18760-18762	DT	denotes	an
T16138	18763-18770	JJ	denotes	obvious
T16139	18771-18774	NN	denotes	DNA
T16141	18774-18775	HYPH	denotes	-
T16140	18775-18782	VBG	denotes	binding
T16137	18783-18789	NN	denotes	domain
T16142	18789-18790	.	denotes	.
T16143	18790-18878	sentence	denotes	To characterize the biochemical activity of mr-s, we next performed a luciferase assay.
T16144	18791-18793	TO	denotes	To
T16145	18794-18806	VB	denotes	characterize
T16147	18807-18810	DT	denotes	the
T16149	18811-18822	JJ	denotes	biochemical
T16148	18823-18831	NN	denotes	activity
T16150	18832-18834	IN	denotes	of
T16151	18835-18837	NN	denotes	mr
T16153	18837-18838	HYPH	denotes	-
T16152	18838-18839	NN	denotes	s
T16154	18839-18841	,	denotes	,
T16155	18841-18843	PRP	denotes	we
T16156	18844-18848	RB	denotes	next
T16146	18849-18858	VBD	denotes	performed
T16157	18859-18860	DT	denotes	a
T16159	18861-18871	NN	denotes	luciferase
T16158	18872-18877	NN	denotes	assay
T16160	18877-18878	.	denotes	.
T16161	18878-19008	sentence	denotes	We generated effector plasmids, which express various deletion constructs of mr-s fused to the GAL4 DNA-binding domain (Fig. 7A).
T16162	18879-18881	PRP	denotes	We
T16163	18882-18891	VBD	denotes	generated
T16164	18892-18900	NN	denotes	effector
T16165	18901-18909	NNS	denotes	plasmids
T16166	18909-18911	,	denotes	,
T16167	18911-18916	WDT	denotes	which
T16168	18917-18924	VBP	denotes	express
T16169	18925-18932	JJ	denotes	various
T16171	18933-18941	NN	denotes	deletion
T16170	18942-18952	NNS	denotes	constructs
T16172	18953-18955	IN	denotes	of
T16173	18956-18958	NN	denotes	mr
T16175	18958-18959	HYPH	denotes	-
T16174	18959-18960	NN	denotes	s
T16176	18961-18966	VBN	denotes	fused
T16177	18967-18969	IN	denotes	to
T16178	18970-18973	DT	denotes	the
T16180	18974-18978	NN	denotes	GAL4
T16181	18979-18982	NN	denotes	DNA
T16183	18982-18983	HYPH	denotes	-
T16182	18983-18990	VBG	denotes	binding
T16179	18991-18997	NN	denotes	domain
T16184	18998-18999	-LRB-	denotes	(
T16186	18999-19003	NN	denotes	Fig.
T16185	19004-19006	NN	denotes	7A
T16187	19006-19007	-RRB-	denotes	)
T16188	19007-19008	.	denotes	.
T16189	19008-19180	sentence	denotes	We first confirmed that full-length mr-s fused to GAL4 DNA binding domain (DBD-mrs) had no effect on the pGL3 promoter plasmid lacking GAL4 binding sites (data not shown).
T16190	19009-19011	PRP	denotes	We
T16192	19012-19017	RB	denotes	first
T16191	19018-19027	VBD	denotes	confirmed
T16193	19028-19032	IN	denotes	that
T16195	19033-19037	JJ	denotes	full
T16197	19037-19038	HYPH	denotes	-
T16196	19038-19044	NN	denotes	length
T16199	19045-19047	NN	denotes	mr
T16200	19047-19048	HYPH	denotes	-
T16198	19048-19049	NN	denotes	s
T16201	19050-19055	VBN	denotes	fused
T16202	19056-19058	IN	denotes	to
T16203	19059-19063	NN	denotes	GAL4
T16205	19064-19067	NN	denotes	DNA
T16206	19068-19075	NN	denotes	binding
T16204	19076-19082	NN	denotes	domain
T16207	19083-19084	-LRB-	denotes	(
T16208	19084-19087	NN	denotes	DBD
T16210	19087-19088	HYPH	denotes	-
T16209	19088-19091	NN	denotes	mrs
T16211	19091-19092	-RRB-	denotes	)
T16194	19093-19096	VBD	denotes	had
T16212	19097-19099	DT	denotes	no
T16213	19100-19106	NN	denotes	effect
T16214	19107-19109	IN	denotes	on
T16215	19110-19113	DT	denotes	the
T16217	19114-19118	NN	denotes	pGL3
T16218	19119-19127	NN	denotes	promoter
T16216	19128-19135	NN	denotes	plasmid
T16219	19136-19143	VBG	denotes	lacking
T16220	19144-19148	NN	denotes	GAL4
T16221	19149-19156	NN	denotes	binding
T16222	19157-19162	NNS	denotes	sites
T16223	19163-19164	-LRB-	denotes	(
T16225	19164-19168	NNS	denotes	data
T16226	19169-19172	RB	denotes	not
T16224	19173-19178	VBN	denotes	shown
T16227	19178-19179	-RRB-	denotes	)
T16228	19179-19180	.	denotes	.
T16229	19180-19327	sentence	denotes	When the 5xGAL4-pGL3 reporter plasmid was co-transfected, DBD-mrs repressed luciferase activity by about 90% in a dose-dependent manner (Fig. 7B).
T16230	19181-19185	WRB	denotes	When
T16232	19186-19189	DT	denotes	the
T16234	19190-19196	NN	denotes	5xGAL4
T16236	19196-19197	HYPH	denotes	-
T16235	19197-19201	NN	denotes	pGL3
T16237	19202-19210	NN	denotes	reporter
T16233	19211-19218	NN	denotes	plasmid
T16238	19219-19222	VBD	denotes	was
T16231	19223-19237	VBN	denotes	co-transfected
T16240	19237-19239	,	denotes	,
T16241	19239-19242	NN	denotes	DBD
T16243	19242-19243	HYPH	denotes	-
T16242	19243-19246	NN	denotes	mrs
T16239	19247-19256	VBN	denotes	repressed
T16244	19257-19267	NN	denotes	luciferase
T16245	19268-19276	NN	denotes	activity
T16246	19277-19279	IN	denotes	by
T16247	19280-19285	IN	denotes	about
T16248	19286-19288	CD	denotes	90
T16249	19288-19289	NN	denotes	%
T16250	19290-19292	IN	denotes	in
T16251	19293-19294	DT	denotes	a
T16253	19295-19299	NN	denotes	dose
T16255	19299-19300	HYPH	denotes	-
T16254	19300-19309	JJ	denotes	dependent
T16252	19310-19316	NN	denotes	manner
T16256	19317-19318	-LRB-	denotes	(
T16258	19318-19322	NN	denotes	Fig.
T16257	19323-19325	NN	denotes	7B
T16259	19325-19326	-RRB-	denotes	)
T16260	19326-19327	.	denotes	.
T16261	19327-19434	sentence	denotes	As a control, GAL4 DNA-binding domain (DBD) had no significant effect on the 5xGAL4-pGL3 reporter plasmid.
T16262	19328-19330	IN	denotes	As
T16264	19331-19332	DT	denotes	a
T16265	19333-19340	NN	denotes	control
T16266	19340-19342	,	denotes	,
T16267	19342-19346	NN	denotes	GAL4
T16269	19347-19350	NN	denotes	DNA
T16271	19350-19351	HYPH	denotes	-
T16270	19351-19358	VBG	denotes	binding
T16268	19359-19365	NN	denotes	domain
T16272	19366-19367	-LRB-	denotes	(
T16273	19367-19370	NN	denotes	DBD
T16274	19370-19371	-RRB-	denotes	)
T16263	19372-19375	VBD	denotes	had
T16275	19376-19378	DT	denotes	no
T16277	19379-19390	JJ	denotes	significant
T16276	19391-19397	NN	denotes	effect
T16278	19398-19400	IN	denotes	on
T16279	19401-19404	DT	denotes	the
T16281	19405-19411	NN	denotes	5xGAL4
T16283	19411-19412	HYPH	denotes	-
T16282	19412-19416	NN	denotes	pGL3
T16284	19417-19425	NN	denotes	reporter
T16280	19426-19433	NN	denotes	plasmid
T16285	19433-19434	.	denotes	.
T16286	19434-19560	sentence	denotes	In addition, we confirmed that full-length mr-s without GAL4 DBD had no effect on the same reporter plasmid (data not shown).
T16287	19435-19437	IN	denotes	In
T16289	19438-19446	NN	denotes	addition
T16290	19446-19448	,	denotes	,
T16291	19448-19450	PRP	denotes	we
T16288	19451-19460	VBD	denotes	confirmed
T16292	19461-19465	IN	denotes	that
T16294	19466-19470	JJ	denotes	full
T16296	19470-19471	HYPH	denotes	-
T16295	19471-19477	NN	denotes	length
T16298	19478-19480	NN	denotes	mr
T16299	19480-19481	HYPH	denotes	-
T16297	19481-19482	NN	denotes	s
T16300	19483-19490	IN	denotes	without
T16301	19491-19495	NN	denotes	GAL4
T16302	19496-19499	NN	denotes	DBD
T16293	19500-19503	VBD	denotes	had
T16303	19504-19506	DT	denotes	no
T16304	19507-19513	NN	denotes	effect
T16305	19514-19516	IN	denotes	on
T16306	19517-19520	DT	denotes	the
T16308	19521-19525	JJ	denotes	same
T16309	19526-19534	NN	denotes	reporter
T16307	19535-19542	NN	denotes	plasmid
T16310	19543-19544	-LRB-	denotes	(
T16312	19544-19548	NNS	denotes	data
T16313	19549-19552	RB	denotes	not
T16311	19553-19558	VBN	denotes	shown
T16314	19558-19559	-RRB-	denotes	)
T16315	19559-19560	.	denotes	.
T16316	19560-19770	sentence	denotes	We next analyzed deletion constructs in which the N-terminus 400 aa stretch of mr-s (amino acids 1 to 400) or the C-terminus portion (amino acids 391 to 542) were fused to the GAL4 DBD (Fig. 7A, DBD-N, DBD-C).
T16317	19561-19563	PRP	denotes	We
T16319	19564-19568	RB	denotes	next
T16318	19569-19577	VBD	denotes	analyzed
T16320	19578-19586	NN	denotes	deletion
T16321	19587-19597	NNS	denotes	constructs
T16322	19598-19600	IN	denotes	in
T16324	19601-19606	WDT	denotes	which
T16325	19607-19610	DT	denotes	the
T16327	19611-19612	NN	denotes	N
T16329	19612-19613	HYPH	denotes	-
T16328	19613-19621	NN	denotes	terminus
T16330	19622-19625	CD	denotes	400
T16331	19626-19628	NN	denotes	aa
T16326	19629-19636	NN	denotes	stretch
T16332	19637-19639	IN	denotes	of
T16333	19640-19642	NN	denotes	mr
T16335	19642-19643	HYPH	denotes	-
T16334	19643-19644	NN	denotes	s
T16336	19645-19646	-LRB-	denotes	(
T16338	19646-19651	NN	denotes	amino
T16339	19652-19657	NNS	denotes	acids
T16337	19658-19659	CD	denotes	1
T16340	19660-19662	IN	denotes	to
T16341	19663-19666	CD	denotes	400
T16342	19666-19667	-RRB-	denotes	)
T16343	19668-19670	CC	denotes	or
T16344	19671-19674	DT	denotes	the
T16346	19675-19676	NN	denotes	C
T16348	19676-19677	HYPH	denotes	-
T16347	19677-19685	NN	denotes	terminus
T16345	19686-19693	NN	denotes	portion
T16349	19694-19695	-LRB-	denotes	(
T16351	19695-19700	NN	denotes	amino
T16352	19701-19706	NNS	denotes	acids
T16350	19707-19710	CD	denotes	391
T16353	19711-19713	IN	denotes	to
T16354	19714-19717	CD	denotes	542
T16355	19717-19718	-RRB-	denotes	)
T16356	19719-19723	VBD	denotes	were
T16323	19724-19729	VBN	denotes	fused
T16357	19730-19732	IN	denotes	to
T16358	19733-19736	DT	denotes	the
T16360	19737-19741	NN	denotes	GAL4
T16359	19742-19745	NN	denotes	DBD
T16361	19746-19747	-LRB-	denotes	(
T16363	19747-19751	NN	denotes	Fig.
T16362	19752-19754	NN	denotes	7A
T16364	19754-19756	,	denotes	,
T16365	19756-19759	NN	denotes	DBD
T16367	19759-19760	HYPH	denotes	-
T16366	19760-19761	NN	denotes	N
T16368	19761-19763	,	denotes	,
T16369	19763-19766	NN	denotes	DBD
T16371	19766-19767	HYPH	denotes	-
T16370	19767-19768	NN	denotes	C
T16372	19768-19769	-RRB-	denotes	)
T16373	19769-19770	.	denotes	.
T16374	19770-19891	sentence	denotes	While DBD-N had no repressive effect on this reporter activity, DBD-C repressed luciferase gene expression by about 65%.
T16375	19771-19776	IN	denotes	While
T16377	19777-19780	NN	denotes	DBD
T16379	19780-19781	HYPH	denotes	-
T16378	19781-19782	NN	denotes	N
T16376	19783-19786	VBD	denotes	had
T16381	19787-19789	DT	denotes	no
T16383	19790-19800	JJ	denotes	repressive
T16382	19801-19807	NN	denotes	effect
T16384	19808-19810	IN	denotes	on
T16385	19811-19815	DT	denotes	this
T16387	19816-19824	NN	denotes	reporter
T16386	19825-19833	NN	denotes	activity
T16388	19833-19835	,	denotes	,
T16389	19835-19838	NN	denotes	DBD
T16391	19838-19839	HYPH	denotes	-
T16390	19839-19840	NN	denotes	C
T16380	19841-19850	VBD	denotes	repressed
T16392	19851-19861	NN	denotes	luciferase
T16394	19862-19866	NN	denotes	gene
T16393	19867-19877	NN	denotes	expression
T16395	19878-19880	IN	denotes	by
T16396	19881-19886	IN	denotes	about
T16397	19887-19889	CD	denotes	65
T16398	19889-19890	NN	denotes	%
T16399	19890-19891	.	denotes	.
T16400	19891-20036	sentence	denotes	This result suggested to us the possibility that DBD-mrs exerts transcriptional repressive activity via self-association through its SAM domain.
T16401	19892-19896	DT	denotes	This
T16402	19897-19903	NN	denotes	result
T16403	19904-19913	VBD	denotes	suggested
T16404	19914-19916	IN	denotes	to
T16405	19917-19919	PRP	denotes	us
T16406	19920-19923	DT	denotes	the
T16407	19924-19935	NN	denotes	possibility
T16408	19936-19940	IN	denotes	that
T16410	19941-19944	NN	denotes	DBD
T16412	19944-19945	HYPH	denotes	-
T16411	19945-19948	NN	denotes	mrs
T16409	19949-19955	VBZ	denotes	exerts
T16413	19956-19971	JJ	denotes	transcriptional
T16415	19972-19982	JJ	denotes	repressive
T16414	19983-19991	NN	denotes	activity
T16416	19992-19995	IN	denotes	via
T16417	19996-20000	NN	denotes	self
T16419	20000-20001	HYPH	denotes	-
T16418	20001-20012	NN	denotes	association
T16420	20013-20020	IN	denotes	through
T16421	20021-20024	PRP$	denotes	its
T16423	20025-20028	NN	denotes	SAM
T16422	20029-20035	NN	denotes	domain
T16424	20035-20036	.	denotes	.
T16425	20036-20264	sentence	denotes	To investigate whether the homophilic association of mr-s is required for transcriptional repression, two site-directed mutants, DBD-W404A and DBD-G453A, either of which may reduce self-binding ability, were analyzed (Fig. 7C).
T16426	20037-20039	TO	denotes	To
T16427	20040-20051	VB	denotes	investigate
T16429	20052-20059	IN	denotes	whether
T16431	20060-20063	DT	denotes	the
T16433	20064-20074	JJ	denotes	homophilic
T16432	20075-20086	NN	denotes	association
T16434	20087-20089	IN	denotes	of
T16435	20090-20092	NN	denotes	mr
T16437	20092-20093	HYPH	denotes	-
T16436	20093-20094	NN	denotes	s
T16438	20095-20097	VBZ	denotes	is
T16430	20098-20106	VBN	denotes	required
T16439	20107-20110	IN	denotes	for
T16440	20111-20126	JJ	denotes	transcriptional
T16441	20127-20137	NN	denotes	repression
T16442	20137-20139	,	denotes	,
T16443	20139-20142	CD	denotes	two
T16445	20143-20147	NN	denotes	site
T16447	20147-20148	HYPH	denotes	-
T16446	20148-20156	VBN	denotes	directed
T16444	20157-20164	NNS	denotes	mutants
T16448	20164-20166	,	denotes	,
T16449	20166-20169	NN	denotes	DBD
T16451	20169-20170	HYPH	denotes	-
T16450	20170-20175	NN	denotes	W404A
T16452	20176-20179	CC	denotes	and
T16453	20180-20183	NN	denotes	DBD
T16455	20183-20184	HYPH	denotes	-
T16454	20184-20189	NN	denotes	G453A
T16456	20189-20191	,	denotes	,
T16457	20191-20197	DT	denotes	either
T16459	20198-20200	IN	denotes	of
T16460	20201-20206	WDT	denotes	which
T16461	20207-20210	MD	denotes	may
T16458	20211-20217	VB	denotes	reduce
T16462	20218-20222	NN	denotes	self
T16464	20222-20223	HYPH	denotes	-
T16463	20223-20230	VBG	denotes	binding
T16465	20231-20238	NN	denotes	ability
T16466	20238-20240	,	denotes	,
T16467	20240-20244	VBD	denotes	were
T16428	20245-20253	VBN	denotes	analyzed
T16468	20254-20255	-LRB-	denotes	(
T16470	20255-20259	NN	denotes	Fig.
T16469	20260-20262	NN	denotes	7C
T16471	20262-20263	-RRB-	denotes	)
T16472	20263-20264	.	denotes	.
T16473	20264-20363	sentence	denotes	Compared to DBD-mrs, DBD-W404A and DBD-G453A had repression activity of 72% and 87%, respectively.
T16474	20265-20273	VBN	denotes	Compared
T16476	20274-20276	IN	denotes	to
T16477	20277-20280	NN	denotes	DBD
T16479	20280-20281	HYPH	denotes	-
T16478	20281-20284	NN	denotes	mrs
T16480	20284-20286	,	denotes	,
T16481	20286-20289	NN	denotes	DBD
T16483	20289-20290	HYPH	denotes	-
T16482	20290-20295	NN	denotes	W404A
T16484	20296-20299	CC	denotes	and
T16485	20300-20303	NN	denotes	DBD
T16487	20303-20304	HYPH	denotes	-
T16486	20304-20309	NN	denotes	G453A
T16475	20310-20313	VBD	denotes	had
T16488	20314-20324	NN	denotes	repression
T16489	20325-20333	NN	denotes	activity
T16490	20334-20336	IN	denotes	of
T16491	20337-20339	CD	denotes	72
T16492	20339-20340	NN	denotes	%
T16493	20341-20344	CC	denotes	and
T16494	20345-20347	CD	denotes	87
T16495	20347-20348	NN	denotes	%
T16496	20348-20350	,	denotes	,
T16497	20350-20362	RB	denotes	respectively
T16498	20362-20363	.	denotes	.
T16499	20363-20550	sentence	denotes	While the ability of mr-s self-association partially correlates with transcriptional repressive activity, these mutants do not compromise the ability to repress transcription critically.
T16500	20364-20369	IN	denotes	While
T16502	20370-20373	DT	denotes	the
T16503	20374-20381	NN	denotes	ability
T16504	20382-20384	IN	denotes	of
T16505	20385-20387	NN	denotes	mr
T16507	20387-20388	HYPH	denotes	-
T16506	20388-20389	NN	denotes	s
T16509	20390-20394	NN	denotes	self
T16510	20394-20395	HYPH	denotes	-
T16508	20395-20406	NN	denotes	association
T16511	20407-20416	RB	denotes	partially
T16501	20417-20427	VBZ	denotes	correlates
T16513	20428-20432	IN	denotes	with
T16514	20433-20448	JJ	denotes	transcriptional
T16516	20449-20459	JJ	denotes	repressive
T16515	20460-20468	NN	denotes	activity
T16517	20468-20470	,	denotes	,
T16518	20470-20475	DT	denotes	these
T16519	20476-20483	NNS	denotes	mutants
T16520	20484-20486	VBP	denotes	do
T16521	20487-20490	RB	denotes	not
T16512	20491-20501	VB	denotes	compromise
T16522	20502-20505	DT	denotes	the
T16523	20506-20513	NN	denotes	ability
T16524	20514-20516	TO	denotes	to
T16525	20517-20524	VB	denotes	repress
T16526	20525-20538	NN	denotes	transcription
T16527	20539-20549	RB	denotes	critically
T16528	20549-20550	.	denotes	.
T16529	20550-20755	sentence	denotes	To determine the regions of mr-s involved in transcriptional repression more precisely, the C-terminus portion was divided into two regions and each was fused to the GAL4 DBD (Fig. 7A, DBD-tail, DBD-SAM).
T16530	20551-20553	TO	denotes	To
T16531	20554-20563	VB	denotes	determine
T16533	20564-20567	DT	denotes	the
T16534	20568-20575	NNS	denotes	regions
T16535	20576-20578	IN	denotes	of
T16536	20579-20581	NN	denotes	mr
T16538	20581-20582	HYPH	denotes	-
T16537	20582-20583	NN	denotes	s
T16539	20584-20592	VBN	denotes	involved
T16540	20593-20595	IN	denotes	in
T16541	20596-20611	JJ	denotes	transcriptional
T16542	20612-20622	NN	denotes	repression
T16543	20623-20627	RBR	denotes	more
T16544	20628-20637	RB	denotes	precisely
T16545	20637-20639	,	denotes	,
T16546	20639-20642	DT	denotes	the
T16548	20643-20644	NN	denotes	C
T16550	20644-20645	HYPH	denotes	-
T16549	20645-20653	NN	denotes	terminus
T16547	20654-20661	NN	denotes	portion
T16551	20662-20665	VBD	denotes	was
T16532	20666-20673	VBN	denotes	divided
T16552	20674-20678	IN	denotes	into
T16553	20679-20682	CD	denotes	two
T16554	20683-20690	NNS	denotes	regions
T16555	20691-20694	CC	denotes	and
T16556	20695-20699	DT	denotes	each
T16558	20700-20703	VBD	denotes	was
T16557	20704-20709	VBN	denotes	fused
T16559	20710-20712	IN	denotes	to
T16560	20713-20716	DT	denotes	the
T16562	20717-20721	NN	denotes	GAL4
T16561	20722-20725	NN	denotes	DBD
T16563	20726-20727	-LRB-	denotes	(
T16565	20727-20731	NN	denotes	Fig.
T16566	20732-20734	NN	denotes	7A
T16567	20734-20736	,	denotes	,
T16568	20736-20739	NN	denotes	DBD
T16570	20739-20740	HYPH	denotes	-
T16569	20740-20744	NN	denotes	tail
T16571	20744-20746	,	denotes	,
T16572	20746-20749	NN	denotes	DBD
T16573	20749-20750	HYPH	denotes	-
T16564	20750-20753	NN	denotes	SAM
T16574	20753-20754	-RRB-	denotes	)
T16575	20754-20755	.	denotes	.
T16576	20755-20876	sentence	denotes	As a consequence, DBD-SAM (amino acids 384 to 462) did not have a repressive effect on the 5xGAL4-pGL3 reporter plasmid.
T16577	20756-20758	IN	denotes	As
T16579	20759-20760	DT	denotes	a
T16580	20761-20772	NN	denotes	consequence
T16581	20772-20774	,	denotes	,
T16582	20774-20777	NN	denotes	DBD
T16584	20777-20778	HYPH	denotes	-
T16583	20778-20781	NN	denotes	SAM
T16585	20782-20783	-LRB-	denotes	(
T16587	20783-20788	NN	denotes	amino
T16588	20789-20794	NNS	denotes	acids
T16586	20795-20798	CD	denotes	384
T16589	20799-20801	IN	denotes	to
T16590	20802-20805	CD	denotes	462
T16591	20805-20806	-RRB-	denotes	)
T16592	20807-20810	VBD	denotes	did
T16593	20811-20814	RB	denotes	not
T16578	20815-20819	VB	denotes	have
T16594	20820-20821	DT	denotes	a
T16596	20822-20832	JJ	denotes	repressive
T16595	20833-20839	NN	denotes	effect
T16597	20840-20842	IN	denotes	on
T16598	20843-20846	DT	denotes	the
T16600	20847-20853	NN	denotes	5xGAL4
T16602	20853-20854	HYPH	denotes	-
T16601	20854-20858	NN	denotes	pGL3
T16603	20859-20867	NN	denotes	reporter
T16599	20868-20875	NN	denotes	plasmid
T16604	20875-20876	.	denotes	.
T16605	20876-21032	sentence	denotes	On the other hand, luciferase activity was repressed by 55% when DBD-tail (amino acids 459 to 542) was co-transfected with this reporter plasmid (Fig. 7D).
T16606	20877-20879	IN	denotes	On
T16608	20880-20883	DT	denotes	the
T16610	20884-20889	JJ	denotes	other
T16609	20890-20894	NN	denotes	hand
T16611	20894-20896	,	denotes	,
T16612	20896-20906	NN	denotes	luciferase
T16613	20907-20915	NN	denotes	activity
T16614	20916-20919	VBD	denotes	was
T16607	20920-20929	VBN	denotes	repressed
T16615	20930-20932	IN	denotes	by
T16616	20933-20935	CD	denotes	55
T16617	20935-20936	NN	denotes	%
T16618	20937-20941	WRB	denotes	when
T16620	20942-20945	NN	denotes	DBD
T16622	20945-20946	HYPH	denotes	-
T16621	20946-20950	NN	denotes	tail
T16623	20951-20952	-LRB-	denotes	(
T16625	20952-20957	NN	denotes	amino
T16626	20958-20963	NNS	denotes	acids
T16624	20964-20967	CD	denotes	459
T16627	20968-20970	IN	denotes	to
T16628	20971-20974	CD	denotes	542
T16629	20974-20975	-RRB-	denotes	)
T16630	20976-20979	VBD	denotes	was
T16619	20980-20994	VBN	denotes	co-transfected
T16631	20995-20999	IN	denotes	with
T16632	21000-21004	DT	denotes	this
T16634	21005-21013	NN	denotes	reporter
T16633	21014-21021	NN	denotes	plasmid
T16635	21022-21023	-LRB-	denotes	(
T16637	21023-21027	NN	denotes	Fig.
T16636	21028-21030	NN	denotes	7D
T16638	21030-21031	-RRB-	denotes	)
T16639	21031-21032	.	denotes	.
T16640	21032-21189	sentence	denotes	To assess the transcriptional repressive activity of mr-s in cells of retinal origin, we performed similar experiments using human Y79 retinoblastoma cells.
T16641	21033-21035	TO	denotes	To
T16642	21036-21042	VB	denotes	assess
T16644	21043-21046	DT	denotes	the
T16646	21047-21062	JJ	denotes	transcriptional
T16647	21063-21073	JJ	denotes	repressive
T16645	21074-21082	NN	denotes	activity
T16648	21083-21085	IN	denotes	of
T16649	21086-21088	NN	denotes	mr
T16651	21088-21089	HYPH	denotes	-
T16650	21089-21090	NN	denotes	s
T16652	21091-21093	IN	denotes	in
T16653	21094-21099	NNS	denotes	cells
T16654	21100-21102	IN	denotes	of
T16655	21103-21110	JJ	denotes	retinal
T16656	21111-21117	NN	denotes	origin
T16657	21117-21119	,	denotes	,
T16658	21119-21121	PRP	denotes	we
T16643	21122-21131	VBD	denotes	performed
T16659	21132-21139	JJ	denotes	similar
T16660	21140-21151	NNS	denotes	experiments
T16661	21152-21157	VBG	denotes	using
T16662	21158-21163	JJ	denotes	human
T16664	21164-21167	NN	denotes	Y79
T16665	21168-21182	NN	denotes	retinoblastoma
T16663	21183-21188	NNS	denotes	cells
T16666	21188-21189	.	denotes	.
T16667	21189-21310	sentence	denotes	The results indicated that DBD-mrs also reduced luciferase activity significantly in Y79 retinoblastoma cells (Fig. 7E).
T16668	21190-21193	DT	denotes	The
T16669	21194-21201	NNS	denotes	results
T16670	21202-21211	VBD	denotes	indicated
T16671	21212-21216	IN	denotes	that
T16673	21217-21220	NN	denotes	DBD
T16675	21220-21221	HYPH	denotes	-
T16674	21221-21224	NN	denotes	mrs
T16676	21225-21229	RB	denotes	also
T16672	21230-21237	VBD	denotes	reduced
T16677	21238-21248	NN	denotes	luciferase
T16678	21249-21257	NN	denotes	activity
T16679	21258-21271	RB	denotes	significantly
T16680	21272-21274	IN	denotes	in
T16681	21275-21278	NN	denotes	Y79
T16683	21279-21293	NN	denotes	retinoblastoma
T16682	21294-21299	NNS	denotes	cells
T16684	21300-21301	-LRB-	denotes	(
T16686	21301-21305	NN	denotes	Fig.
T16685	21306-21308	NN	denotes	7E
T16687	21308-21309	-RRB-	denotes	)
T16688	21309-21310	.	denotes	.
T16689	21310-21428	sentence	denotes	However, luciferase activity was repressed by about 30% in Y79 cells, while it was repressed by 90% in HEK293T cells.
T16690	21311-21318	RB	denotes	However
T16692	21318-21320	,	denotes	,
T16693	21320-21330	NN	denotes	luciferase
T16694	21331-21339	NN	denotes	activity
T16695	21340-21343	VBD	denotes	was
T16691	21344-21353	VBN	denotes	repressed
T16696	21354-21356	IN	denotes	by
T16697	21357-21362	IN	denotes	about
T16698	21363-21365	CD	denotes	30
T16699	21365-21366	NN	denotes	%
T16700	21367-21369	IN	denotes	in
T16701	21370-21373	NN	denotes	Y79
T16702	21374-21379	NNS	denotes	cells
T16703	21379-21381	,	denotes	,
T16704	21381-21386	IN	denotes	while
T16706	21387-21389	PRP	denotes	it
T16707	21390-21393	VBD	denotes	was
T16705	21394-21403	VBN	denotes	repressed
T16708	21404-21406	IN	denotes	by
T16709	21407-21409	CD	denotes	90
T16710	21409-21410	NN	denotes	%
T16711	21411-21413	IN	denotes	in
T16712	21414-21421	NN	denotes	HEK293T
T16713	21422-21427	NNS	denotes	cells
T16714	21427-21428	.	denotes	.
T16715	21428-21517	sentence	denotes	This might be due to the difference in transfection efficiency between these cell lines.
T16716	21429-21433	DT	denotes	This
T16718	21434-21439	MD	denotes	might
T16717	21440-21442	VB	denotes	be
T16719	21443-21446	IN	denotes	due
T16720	21447-21449	IN	denotes	to
T16721	21450-21453	DT	denotes	the
T16722	21454-21464	NN	denotes	difference
T16723	21465-21467	IN	denotes	in
T16724	21468-21480	NN	denotes	transfection
T16725	21481-21491	NN	denotes	efficiency
T16726	21492-21499	IN	denotes	between
T16727	21500-21505	DT	denotes	these
T16729	21506-21510	NN	denotes	cell
T16728	21511-21516	NNS	denotes	lines
T16730	21516-21517	.	denotes	.
T16731	21517-21675	sentence	denotes	Another possibility is that intracellular environment in Y79 cells, a retinoblastoma cell line, is insufficient for recapitulating developing photoreceptors.
T16732	21518-21525	DT	denotes	Another
T16733	21526-21537	NN	denotes	possibility
T16734	21538-21540	VBZ	denotes	is
T16735	21541-21545	IN	denotes	that
T16737	21546-21559	JJ	denotes	intracellular
T16738	21560-21571	NN	denotes	environment
T16739	21572-21574	IN	denotes	in
T16740	21575-21578	NN	denotes	Y79
T16741	21579-21584	NNS	denotes	cells
T16742	21584-21586	,	denotes	,
T16743	21586-21587	DT	denotes	a
T16745	21588-21602	NN	denotes	retinoblastoma
T16746	21603-21607	NN	denotes	cell
T16744	21608-21612	NN	denotes	line
T16747	21612-21614	,	denotes	,
T16736	21614-21616	VBZ	denotes	is
T16748	21617-21629	JJ	denotes	insufficient
T16749	21630-21633	IN	denotes	for
T16750	21634-21648	VBG	denotes	recapitulating
T16751	21649-21659	VBG	denotes	developing
T16752	21660-21674	NNS	denotes	photoreceptors
T16753	21674-21675	.	denotes	.
T16754	21675-21811	sentence	denotes	In this study, we did not address the question whether or not our in vitro data reflect native mr-s transcriptional repression in vivo.
T16755	21676-21678	IN	denotes	In
T16757	21679-21683	DT	denotes	this
T16758	21684-21689	NN	denotes	study
T16759	21689-21691	,	denotes	,
T16760	21691-21693	PRP	denotes	we
T16761	21694-21697	VBD	denotes	did
T16762	21698-21701	RB	denotes	not
T16756	21702-21709	VB	denotes	address
T16763	21710-21713	DT	denotes	the
T16764	21714-21722	NN	denotes	question
T16765	21723-21730	IN	denotes	whether
T16767	21731-21733	CC	denotes	or
T16768	21734-21737	RB	denotes	not
T16769	21738-21741	PRP$	denotes	our
T16771	21742-21744	FW	denotes	in
T16772	21745-21750	FW	denotes	vitro
T16770	21751-21755	NNS	denotes	data
T16766	21756-21763	VBP	denotes	reflect
T16773	21764-21770	JJ	denotes	native
T16775	21771-21773	NN	denotes	mr
T16777	21773-21774	HYPH	denotes	-
T16776	21774-21775	NN	denotes	s
T16778	21776-21791	JJ	denotes	transcriptional
T16774	21792-21802	NN	denotes	repression
T16779	21803-21805	FW	denotes	in
T16780	21806-21810	FW	denotes	vivo
T16781	21810-21811	.	denotes	.
T16782	21811-21988	sentence	denotes	However, these in vitro experiments using HEK293T and Y79 cells strongly support our hypothesis that mr-s functions as a transcriptional repressor in developing photoreceptors.
T16783	21812-21819	RB	denotes	However
T16785	21819-21821	,	denotes	,
T16786	21821-21826	DT	denotes	these
T16788	21827-21829	FW	denotes	in
T16789	21830-21835	FW	denotes	vitro
T16787	21836-21847	NNS	denotes	experiments
T16790	21848-21853	VBG	denotes	using
T16791	21854-21861	NN	denotes	HEK293T
T16793	21862-21865	CC	denotes	and
T16794	21866-21869	NN	denotes	Y79
T16792	21870-21875	NNS	denotes	cells
T16795	21876-21884	RB	denotes	strongly
T16784	21885-21892	VBP	denotes	support
T16796	21893-21896	PRP$	denotes	our
T16797	21897-21907	NN	denotes	hypothesis
T16798	21908-21912	IN	denotes	that
T16800	21913-21915	NN	denotes	mr
T16802	21915-21916	HYPH	denotes	-
T16801	21916-21917	NN	denotes	s
T16799	21918-21927	VBZ	denotes	functions
T16803	21928-21930	IN	denotes	as
T16804	21931-21932	DT	denotes	a
T16806	21933-21948	JJ	denotes	transcriptional
T16805	21949-21958	NN	denotes	repressor
T16807	21959-21961	IN	denotes	in
T16808	21962-21972	VBG	denotes	developing
T16809	21973-21987	NNS	denotes	photoreceptors
T16810	21987-21988	.	denotes	.
T16811	21988-22196	sentence	denotes	Our results suggested that the C-terminal region of mr-s (amino acids 463 to 542) is required for transcriptional repression of mr-s and the SAM domain appears to be dispensable for this repressive activity.
T16812	21989-21992	PRP$	denotes	Our
T16813	21993-22000	NNS	denotes	results
T16814	22001-22010	VBD	denotes	suggested
T16815	22011-22015	IN	denotes	that
T16817	22016-22019	DT	denotes	the
T16819	22020-22021	NN	denotes	C
T16821	22021-22022	HYPH	denotes	-
T16820	22022-22030	JJ	denotes	terminal
T16818	22031-22037	NN	denotes	region
T16822	22038-22040	IN	denotes	of
T16823	22041-22043	NN	denotes	mr
T16825	22043-22044	HYPH	denotes	-
T16824	22044-22045	NN	denotes	s
T16826	22046-22047	-LRB-	denotes	(
T16828	22047-22052	NN	denotes	amino
T16829	22053-22058	NNS	denotes	acids
T16827	22059-22062	CD	denotes	463
T16830	22063-22065	IN	denotes	to
T16831	22066-22069	CD	denotes	542
T16832	22069-22070	-RRB-	denotes	)
T16833	22071-22073	VBZ	denotes	is
T16816	22074-22082	VBN	denotes	required
T16834	22083-22086	IN	denotes	for
T16835	22087-22102	JJ	denotes	transcriptional
T16836	22103-22113	NN	denotes	repression
T16837	22114-22116	IN	denotes	of
T16838	22117-22119	NN	denotes	mr
T16840	22119-22120	HYPH	denotes	-
T16839	22120-22121	NN	denotes	s
T16841	22122-22125	CC	denotes	and
T16842	22126-22129	DT	denotes	the
T16844	22130-22133	NN	denotes	SAM
T16843	22134-22140	NN	denotes	domain
T16845	22141-22148	VBZ	denotes	appears
T16846	22149-22151	TO	denotes	to
T16847	22152-22154	VB	denotes	be
T16848	22155-22166	JJ	denotes	dispensable
T16849	22167-22170	IN	denotes	for
T16850	22171-22175	DT	denotes	this
T16852	22176-22186	JJ	denotes	repressive
T16851	22187-22195	NN	denotes	activity
T16853	22195-22196	.	denotes	.
T16854	22196-22278	sentence	denotes	This C-terminal region of mouse mr-s is highly conserved among species (Fig. 7F).
T16855	22197-22201	DT	denotes	This
T16857	22202-22203	NN	denotes	C
T16859	22203-22204	HYPH	denotes	-
T16858	22204-22212	JJ	denotes	terminal
T16856	22213-22219	NN	denotes	region
T16861	22220-22222	IN	denotes	of
T16862	22223-22228	NN	denotes	mouse
T16864	22229-22231	NN	denotes	mr
T16865	22231-22232	HYPH	denotes	-
T16863	22232-22233	NN	denotes	s
T16866	22234-22236	VBZ	denotes	is
T16867	22237-22243	RB	denotes	highly
T16860	22244-22253	VBN	denotes	conserved
T16868	22254-22259	IN	denotes	among
T16869	22260-22267	NNS	denotes	species
T16870	22268-22269	-LRB-	denotes	(
T16872	22269-22273	NN	denotes	Fig.
T16871	22274-22276	NN	denotes	7F
T16873	22276-22277	-RRB-	denotes	)
T16874	22277-22278	.	denotes	.
T16875	22278-22401	sentence	denotes	The sequence identity of the region was 93%, 73%, 41% and 40% for rat, human, chick and zebrafish, respectively (Fig. 1D).
T16876	22279-22282	DT	denotes	The
T16878	22283-22291	NN	denotes	sequence
T16877	22292-22300	NN	denotes	identity
T16880	22301-22303	IN	denotes	of
T16881	22304-22307	DT	denotes	the
T16882	22308-22314	NN	denotes	region
T16879	22315-22318	VBD	denotes	was
T16883	22319-22321	CD	denotes	93
T16884	22321-22322	NN	denotes	%
T16885	22322-22324	,	denotes	,
T16886	22324-22326	CD	denotes	73
T16887	22326-22327	NN	denotes	%
T16888	22327-22329	,	denotes	,
T16889	22329-22331	CD	denotes	41
T16890	22331-22332	NN	denotes	%
T16891	22333-22336	CC	denotes	and
T16892	22337-22339	CD	denotes	40
T16893	22339-22340	NN	denotes	%
T16894	22341-22344	IN	denotes	for
T16895	22345-22348	NN	denotes	rat
T16896	22348-22350	,	denotes	,
T16897	22350-22355	JJ	denotes	human
T16898	22355-22357	,	denotes	,
T16899	22357-22362	NN	denotes	chick
T16900	22363-22366	CC	denotes	and
T16901	22367-22376	NN	denotes	zebrafish
T16902	22376-22378	,	denotes	,
T16903	22378-22390	RB	denotes	respectively
T16904	22391-22392	-LRB-	denotes	(
T16906	22392-22396	NN	denotes	Fig.
T16905	22397-22399	NN	denotes	1D
T16907	22399-22400	-RRB-	denotes	)
T16908	22400-22401	.	denotes	.
T16909	22401-22544	sentence	denotes	This strongly suggests that the C-terminal region of mouse mr-s functions as a transcriptional repressive domain in photoreceptor development.
T16910	22402-22406	DT	denotes	This
T16912	22407-22415	RB	denotes	strongly
T16911	22416-22424	VBZ	denotes	suggests
T16913	22425-22429	IN	denotes	that
T16915	22430-22433	DT	denotes	the
T16917	22434-22435	NN	denotes	C
T16919	22435-22436	HYPH	denotes	-
T16918	22436-22444	JJ	denotes	terminal
T16916	22445-22451	NN	denotes	region
T16920	22452-22454	IN	denotes	of
T16921	22455-22460	NN	denotes	mouse
T16923	22461-22463	NN	denotes	mr
T16924	22463-22464	HYPH	denotes	-
T16922	22464-22465	NN	denotes	s
T16914	22466-22475	VBZ	denotes	functions
T16925	22476-22478	IN	denotes	as
T16926	22479-22480	DT	denotes	a
T16928	22481-22496	JJ	denotes	transcriptional
T16929	22497-22507	JJ	denotes	repressive
T16927	22508-22514	NN	denotes	domain
T16930	22515-22517	IN	denotes	in
T16931	22518-22531	NN	denotes	photoreceptor
T16932	22532-22543	NN	denotes	development
T16933	22543-22544	.	denotes	.
T16934	22544-22739	sentence	denotes	However, this region does not contain characteristic amino acid motifs and the mechanism through which the region achieves and/or maintains gene repression remains to be clarified in the future.
T16935	22545-22552	RB	denotes	However
T16937	22552-22554	,	denotes	,
T16938	22554-22558	DT	denotes	this
T16939	22559-22565	NN	denotes	region
T16940	22566-22570	VBZ	denotes	does
T16941	22571-22574	RB	denotes	not
T16936	22575-22582	VB	denotes	contain
T16942	22583-22597	JJ	denotes	characteristic
T16943	22598-22603	NN	denotes	amino
T16944	22604-22608	NN	denotes	acid
T16945	22609-22615	NNS	denotes	motifs
T16946	22616-22619	CC	denotes	and
T16947	22620-22623	DT	denotes	the
T16948	22624-22633	NN	denotes	mechanism
T16950	22634-22641	IN	denotes	through
T16952	22642-22647	WDT	denotes	which
T16953	22648-22651	DT	denotes	the
T16954	22652-22658	NN	denotes	region
T16951	22659-22667	VBZ	denotes	achieves
T16955	22668-22671	CC	denotes	and
T16956	22671-22672	HYPH	denotes	/
T16957	22672-22674	CC	denotes	or
T16958	22675-22684	VBZ	denotes	maintains
T16959	22685-22689	NN	denotes	gene
T16960	22690-22700	NN	denotes	repression
T16949	22701-22708	VBZ	denotes	remains
T16961	22709-22711	TO	denotes	to
T16963	22712-22714	VB	denotes	be
T16962	22715-22724	VBN	denotes	clarified
T16964	22725-22727	IN	denotes	in
T16965	22728-22731	DT	denotes	the
T16966	22732-22738	NN	denotes	future
T16967	22738-22739	.	denotes	.
T16968	22739-24422	sentence	denotes	Figure 7 mr-s fused to GAL4 DNA binding domain functions as a transcriptional repressor in HEK293T cells. (A) Schematic drawing of the constructs used for the luciferase assay. 5xGAL4-pGL3 reporter plasmid was co-transfected into HEK293T cells with effector plasmids expressing various deletion mutants fused to GAL4-DBD. (B) Various amounts of DBD, DBD-mrs, DBD-N or DBD-C plasmids were transfected with 0.1 μg of 5xGAL4-pGL3 reporter plasmid. The reporter activity in the presence of the pcDNA3 vector (pcDNA3) was designated as 1. Error bars represent standard error of mean. (C) DBD-W404A and DBD-G453A were co-transfected into HEK293T cells with 5xGAL4-pGL3 reporter plasmid. Fold repression was calculated as the fold decrease in luciferase activity compared with DBD-mrs. Error bars represent standard deviation. (D) Various amounts of DBD-tail or DBD-SAM were transfected with 5xGAL4-pGL3 reporter plasmid. Error bars represent standard error of mean. (E) pcDNA3 or DBD-mrs (5 μg) was co-transfected into Y79 retinoblastoma cells with 0.5 μg of 5xGAL4-pGL3 reporter plasmid. The reporter activity in the presence of pcDNA3 was designated as 1. Error bars represent standard deviation. Asterisk marks statistically significant difference (Student's t test: p < 0.03). (F) Alignment of the C-terminal regions for mouse, rat, human, chick and zebrafish mr-s proteins. Conserved amino acid residues are shown with a dark shadow. Taken together, our findings suggest that DBD-mrs functions as a transcriptional repressor and that the repression activity of mr-s is not due to a homophilic interaction through its SAM domain but to the C-terminal region (amino acids 463 to 542).
T32444	22750-22752	NN	denotes	mr
T32446	22752-22753	HYPH	denotes	-
T32445	22753-22754	NN	denotes	s
T32448	22755-22760	VBN	denotes	fused
T32449	22761-22763	IN	denotes	to
T32450	22764-22768	NN	denotes	GAL4
T32452	22769-22772	NN	denotes	DNA
T32453	22773-22780	VBG	denotes	binding
T32451	22781-22787	NN	denotes	domain
T32447	22788-22797	VBZ	denotes	functions
T32454	22798-22800	IN	denotes	as
T32455	22801-22802	DT	denotes	a
T32457	22803-22818	JJ	denotes	transcriptional
T32456	22819-22828	NN	denotes	repressor
T32458	22829-22831	IN	denotes	in
T32459	22832-22839	NN	denotes	HEK293T
T32460	22840-22845	NNS	denotes	cells
T32461	22845-22846	.	denotes	.
T32462	22846-22917	sentence	denotes	(A) Schematic drawing of the constructs used for the luciferase assay.
T32463	22847-22848	-LRB-	denotes	(
T32464	22848-22849	LS	denotes	A
T32466	22849-22850	-RRB-	denotes	)
T32467	22851-22860	JJ	denotes	Schematic
T32465	22861-22868	NN	denotes	drawing
T32468	22869-22871	IN	denotes	of
T32469	22872-22875	DT	denotes	the
T32470	22876-22886	NNS	denotes	constructs
T32471	22887-22891	VBN	denotes	used
T32472	22892-22895	IN	denotes	for
T32473	22896-22899	DT	denotes	the
T32475	22900-22910	NN	denotes	luciferase
T32474	22911-22916	NN	denotes	assay
T32476	22916-22917	.	denotes	.
T32477	22917-23062	sentence	denotes	5xGAL4-pGL3 reporter plasmid was co-transfected into HEK293T cells with effector plasmids expressing various deletion mutants fused to GAL4-DBD.
T32478	22918-22924	NN	denotes	5xGAL4
T32480	22924-22925	HYPH	denotes	-
T32479	22925-22929	NN	denotes	pGL3
T32482	22930-22938	NN	denotes	reporter
T32481	22939-22946	NN	denotes	plasmid
T32484	22947-22950	VBD	denotes	was
T32483	22951-22965	VBN	denotes	co-transfected
T32485	22966-22970	IN	denotes	into
T32486	22971-22978	NN	denotes	HEK293T
T32487	22979-22984	NNS	denotes	cells
T32488	22985-22989	IN	denotes	with
T32489	22990-22998	NN	denotes	effector
T32490	22999-23007	NNS	denotes	plasmids
T32491	23008-23018	VBG	denotes	expressing
T32492	23019-23026	JJ	denotes	various
T32494	23027-23035	NN	denotes	deletion
T32493	23036-23043	NNS	denotes	mutants
T32495	23044-23049	VBN	denotes	fused
T32496	23050-23052	IN	denotes	to
T32497	23053-23057	NN	denotes	GAL4
T32499	23057-23058	HYPH	denotes	-
T32498	23058-23061	NN	denotes	DBD
T32500	23061-23062	.	denotes	.
T32501	23062-23185	sentence	denotes	(B) Various amounts of DBD, DBD-mrs, DBD-N or DBD-C plasmids were transfected with 0.1 μg of 5xGAL4-pGL3 reporter plasmid.
T32502	23063-23064	-LRB-	denotes	(
T32503	23064-23065	LS	denotes	B
T32505	23065-23066	-RRB-	denotes	)
T32506	23067-23074	JJ	denotes	Various
T32507	23075-23082	NNS	denotes	amounts
T32508	23083-23085	IN	denotes	of
T32509	23086-23089	NN	denotes	DBD
T32511	23089-23091	,	denotes	,
T32512	23091-23094	NN	denotes	DBD
T32514	23094-23095	HYPH	denotes	-
T32513	23095-23098	NN	denotes	mrs
T32515	23098-23100	,	denotes	,
T32516	23100-23103	NN	denotes	DBD
T32518	23103-23104	HYPH	denotes	-
T32517	23104-23105	NN	denotes	N
T32519	23106-23108	CC	denotes	or
T32520	23109-23112	NN	denotes	DBD
T32522	23112-23113	HYPH	denotes	-
T32521	23113-23114	NN	denotes	C
T32510	23115-23123	NNS	denotes	plasmids
T32523	23124-23128	VBD	denotes	were
T32504	23129-23140	VBN	denotes	transfected
T32524	23141-23145	IN	denotes	with
T32525	23146-23149	CD	denotes	0.1
T32526	23150-23152	NN	denotes	μg
T32527	23153-23155	IN	denotes	of
T32528	23156-23162	NN	denotes	5xGAL4
T32530	23162-23163	HYPH	denotes	-
T32529	23163-23167	NN	denotes	pGL3
T32532	23168-23176	NN	denotes	reporter
T32531	23177-23184	NN	denotes	plasmid
T32533	23184-23185	.	denotes	.
T32534	23185-23274	sentence	denotes	The reporter activity in the presence of the pcDNA3 vector (pcDNA3) was designated as 1.
T32535	23186-23189	DT	denotes	The
T32537	23190-23198	NN	denotes	reporter
T32536	23199-23207	NN	denotes	activity
T32539	23208-23210	IN	denotes	in
T32540	23211-23214	DT	denotes	the
T32541	23215-23223	NN	denotes	presence
T32542	23224-23226	IN	denotes	of
T32543	23227-23230	DT	denotes	the
T32545	23231-23237	NN	denotes	pcDNA3
T32544	23238-23244	NN	denotes	vector
T32546	23245-23246	-LRB-	denotes	(
T32547	23246-23252	NN	denotes	pcDNA3
T32548	23252-23253	-RRB-	denotes	)
T32549	23254-23257	VBD	denotes	was
T32538	23258-23268	VBN	denotes	designated
T32550	23269-23271	IN	denotes	as
T32551	23272-23273	CD	denotes	1
T32552	23273-23274	.	denotes	.
T32553	23274-23319	sentence	denotes	Error bars represent standard error of mean.
T32554	23275-23280	NN	denotes	Error
T32555	23281-23285	NNS	denotes	bars
T32556	23286-23295	VBP	denotes	represent
T32557	23296-23304	JJ	denotes	standard
T32558	23305-23310	NN	denotes	error
T32559	23311-23313	IN	denotes	of
T32560	23314-23318	NN	denotes	mean
T32561	23318-23319	.	denotes	.
T32562	23319-23421	sentence	denotes	(C) DBD-W404A and DBD-G453A were co-transfected into HEK293T cells with 5xGAL4-pGL3 reporter plasmid.
T32563	23320-23321	-LRB-	denotes	(
T32564	23321-23322	LS	denotes	C
T32566	23322-23323	-RRB-	denotes	)
T32567	23324-23327	NN	denotes	DBD
T32569	23327-23328	HYPH	denotes	-
T32568	23328-23333	NN	denotes	W404A
T32570	23334-23337	CC	denotes	and
T32571	23338-23341	NN	denotes	DBD
T32573	23341-23342	HYPH	denotes	-
T32572	23342-23347	NN	denotes	G453A
T32574	23348-23352	VBD	denotes	were
T32565	23353-23367	VBN	denotes	co-transfected
T32575	23368-23372	IN	denotes	into
T32576	23373-23380	NN	denotes	HEK293T
T32577	23381-23386	NNS	denotes	cells
T32578	23387-23391	IN	denotes	with
T32579	23392-23398	NN	denotes	5xGAL4
T32581	23398-23399	HYPH	denotes	-
T32580	23399-23403	NN	denotes	pGL3
T32583	23404-23412	NN	denotes	reporter
T32582	23413-23420	NN	denotes	plasmid
T32584	23420-23421	.	denotes	.
T32585	23421-23519	sentence	denotes	Fold repression was calculated as the fold decrease in luciferase activity compared with DBD-mrs.
T32586	23422-23426	NN	denotes	Fold
T32587	23427-23437	NN	denotes	repression
T32589	23438-23441	VBD	denotes	was
T32588	23442-23452	VBN	denotes	calculated
T32590	23453-23455	IN	denotes	as
T32591	23456-23459	DT	denotes	the
T32593	23460-23464	NN	denotes	fold
T32592	23465-23473	NN	denotes	decrease
T32594	23474-23476	IN	denotes	in
T32595	23477-23487	NN	denotes	luciferase
T32596	23488-23496	NN	denotes	activity
T32597	23497-23505	VBN	denotes	compared
T32598	23506-23510	IN	denotes	with
T32599	23511-23514	NN	denotes	DBD
T32601	23514-23515	HYPH	denotes	-
T32600	23515-23518	NN	denotes	mrs
T32602	23518-23519	.	denotes	.
T32603	23519-23560	sentence	denotes	Error bars represent standard deviation.
T32604	23520-23525	NN	denotes	Error
T32605	23526-23530	NNS	denotes	bars
T32606	23531-23540	VBP	denotes	represent
T32607	23541-23549	JJ	denotes	standard
T32608	23550-23559	NN	denotes	deviation
T32609	23559-23560	.	denotes	.
T32610	23560-23655	sentence	denotes	(D) Various amounts of DBD-tail or DBD-SAM were transfected with 5xGAL4-pGL3 reporter plasmid.
T32611	23561-23562	-LRB-	denotes	(
T32612	23562-23563	LS	denotes	D
T32614	23563-23564	-RRB-	denotes	)
T32615	23565-23572	JJ	denotes	Various
T32616	23573-23580	NNS	denotes	amounts
T32617	23581-23583	IN	denotes	of
T32618	23584-23587	NN	denotes	DBD
T32620	23587-23588	HYPH	denotes	-
T32619	23588-23592	NN	denotes	tail
T32621	23593-23595	CC	denotes	or
T32622	23596-23599	NN	denotes	DBD
T32624	23599-23600	HYPH	denotes	-
T32623	23600-23603	NN	denotes	SAM
T32625	23604-23608	VBD	denotes	were
T32613	23609-23620	VBN	denotes	transfected
T32626	23621-23625	IN	denotes	with
T32627	23626-23632	NN	denotes	5xGAL4
T32629	23632-23633	HYPH	denotes	-
T32628	23633-23637	NN	denotes	pGL3
T32631	23638-23646	NN	denotes	reporter
T32630	23647-23654	NN	denotes	plasmid
T32632	23654-23655	.	denotes	.
T32633	23655-23700	sentence	denotes	Error bars represent standard error of mean.
T32634	23656-23661	NN	denotes	Error
T32635	23662-23666	NNS	denotes	bars
T32636	23667-23676	VBP	denotes	represent
T32637	23677-23685	JJ	denotes	standard
T32638	23686-23691	NN	denotes	error
T32639	23692-23694	IN	denotes	of
T32640	23695-23699	NN	denotes	mean
T32641	23699-23700	.	denotes	.
T32642	23700-23823	sentence	denotes	(E) pcDNA3 or DBD-mrs (5 μg) was co-transfected into Y79 retinoblastoma cells with 0.5 μg of 5xGAL4-pGL3 reporter plasmid.
T32643	23701-23702	-LRB-	denotes	(
T32644	23702-23703	LS	denotes	E
T32646	23703-23704	-RRB-	denotes	)
T32647	23705-23711	NN	denotes	pcDNA3
T32648	23712-23714	CC	denotes	or
T32649	23715-23718	NN	denotes	DBD
T32651	23718-23719	HYPH	denotes	-
T32650	23719-23722	NN	denotes	mrs
T32652	23723-23724	-LRB-	denotes	(
T32654	23724-23725	CD	denotes	5
T32653	23726-23728	NN	denotes	μg
T32655	23728-23729	-RRB-	denotes	)
T32656	23730-23733	VBD	denotes	was
T32645	23734-23748	VBN	denotes	co-transfected
T32657	23749-23753	IN	denotes	into
T32658	23754-23757	NN	denotes	Y79
T32660	23758-23772	NN	denotes	retinoblastoma
T32659	23773-23778	NNS	denotes	cells
T32661	23779-23783	IN	denotes	with
T32662	23784-23787	CD	denotes	0.5
T32663	23788-23790	NN	denotes	μg
T32664	23791-23793	IN	denotes	of
T32665	23794-23800	NN	denotes	5xGAL4
T32667	23800-23801	HYPH	denotes	-
T32666	23801-23805	NN	denotes	pGL3
T32669	23806-23814	NN	denotes	reporter
T32668	23815-23822	NN	denotes	plasmid
T32670	23822-23823	.	denotes	.
T32671	23823-23892	sentence	denotes	The reporter activity in the presence of pcDNA3 was designated as 1.
T32672	23824-23827	DT	denotes	The
T32674	23828-23836	NN	denotes	reporter
T32673	23837-23845	NN	denotes	activity
T32676	23846-23848	IN	denotes	in
T32677	23849-23852	DT	denotes	the
T32678	23853-23861	NN	denotes	presence
T32679	23862-23864	IN	denotes	of
T32680	23865-23871	NN	denotes	pcDNA3
T32681	23872-23875	VBD	denotes	was
T32675	23876-23886	VBN	denotes	designated
T32682	23887-23889	IN	denotes	as
T32683	23890-23891	CD	denotes	1
T32684	23891-23892	.	denotes	.
T32685	23892-23933	sentence	denotes	Error bars represent standard deviation.
T32686	23893-23898	NN	denotes	Error
T32687	23899-23903	NNS	denotes	bars
T32688	23904-23913	VBP	denotes	represent
T32689	23914-23922	JJ	denotes	standard
T32690	23923-23932	NN	denotes	deviation
T32691	23932-23933	.	denotes	.
T32692	23933-24015	sentence	denotes	Asterisk marks statistically significant difference (Student's t test: p < 0.03).
T32693	23934-23942	NN	denotes	Asterisk
T32694	23943-23948	VBZ	denotes	marks
T32695	23949-23962	RB	denotes	statistically
T32696	23963-23974	JJ	denotes	significant
T32697	23975-23985	NN	denotes	difference
T32698	23986-23987	-LRB-	denotes	(
T32700	23987-23994	NNP	denotes	Student
T32701	23994-23996	POS	denotes	's
T32702	23997-23998	NN	denotes	t
T32699	23999-24003	NN	denotes	test
T32703	24003-24005	:	denotes	:
T32704	24005-24006	NN	denotes	p
T32706	24007-24008	SYM	denotes	<
T32705	24009-24013	CD	denotes	0.03
T32707	24013-24014	-RRB-	denotes	)
T32708	24014-24015	.	denotes	.
T32709	24015-24113	sentence	denotes	(F) Alignment of the C-terminal regions for mouse, rat, human, chick and zebrafish mr-s proteins.
T32710	24016-24017	-LRB-	denotes	(
T32711	24017-24018	LS	denotes	F
T32713	24018-24019	-RRB-	denotes	)
T32712	24020-24029	NN	denotes	Alignment
T32714	24030-24032	IN	denotes	of
T32715	24033-24036	DT	denotes	the
T32717	24037-24038	NN	denotes	C
T32719	24038-24039	HYPH	denotes	-
T32718	24039-24047	JJ	denotes	terminal
T32716	24048-24055	NNS	denotes	regions
T32720	24056-24059	IN	denotes	for
T32721	24060-24065	NN	denotes	mouse
T32723	24065-24067	,	denotes	,
T32724	24067-24070	NN	denotes	rat
T32725	24070-24072	,	denotes	,
T32726	24072-24077	JJ	denotes	human
T32727	24077-24079	,	denotes	,
T32728	24079-24084	NN	denotes	chick
T32729	24085-24088	CC	denotes	and
T32730	24089-24098	NN	denotes	zebrafish
T32731	24099-24101	NN	denotes	mr
T32733	24101-24102	HYPH	denotes	-
T32732	24102-24103	NN	denotes	s
T32722	24104-24112	NN	denotes	proteins
T32734	24112-24113	.	denotes	.
T32735	24113-24173	sentence	denotes	Conserved amino acid residues are shown with a dark shadow.
T32736	24114-24123	VBN	denotes	Conserved
T32738	24124-24129	NN	denotes	amino
T32739	24130-24134	NN	denotes	acid
T32737	24135-24143	NNS	denotes	residues
T32741	24144-24147	VBP	denotes	are
T32740	24148-24153	VBN	denotes	shown
T32742	24154-24158	IN	denotes	with
T32743	24159-24160	DT	denotes	a
T32745	24161-24165	JJ	denotes	dark
T32744	24166-24172	NN	denotes	shadow
T32746	24172-24173	.	denotes	.
T16969	24174-24179	VBN	denotes	Taken
T16971	24180-24188	RB	denotes	together
T16972	24188-24190	,	denotes	,
T16973	24190-24193	PRP$	denotes	our
T16974	24194-24202	NNS	denotes	findings
T16970	24203-24210	VBP	denotes	suggest
T16975	24211-24215	IN	denotes	that
T16977	24216-24219	NN	denotes	DBD
T16979	24219-24220	HYPH	denotes	-
T16978	24220-24223	NN	denotes	mrs
T16976	24224-24233	VBZ	denotes	functions
T16980	24234-24236	IN	denotes	as
T16981	24237-24238	DT	denotes	a
T16983	24239-24254	JJ	denotes	transcriptional
T16982	24255-24264	NN	denotes	repressor
T16984	24265-24268	CC	denotes	and
T16985	24269-24273	IN	denotes	that
T16987	24274-24277	DT	denotes	the
T16989	24278-24288	NN	denotes	repression
T16988	24289-24297	NN	denotes	activity
T16990	24298-24300	IN	denotes	of
T16991	24301-24303	NN	denotes	mr
T16993	24303-24304	HYPH	denotes	-
T16992	24304-24305	NN	denotes	s
T16986	24306-24308	VBZ	denotes	is
T16994	24309-24312	RB	denotes	not
T16995	24313-24316	IN	denotes	due
T16996	24317-24319	IN	denotes	to
T16997	24320-24321	DT	denotes	a
T16999	24322-24332	JJ	denotes	homophilic
T16998	24333-24344	NN	denotes	interaction
T17000	24345-24352	IN	denotes	through
T17001	24353-24356	PRP$	denotes	its
T17003	24357-24360	NN	denotes	SAM
T17002	24361-24367	NN	denotes	domain
T17004	24368-24371	CC	denotes	but
T17005	24372-24374	IN	denotes	to
T17006	24375-24378	DT	denotes	the
T17008	24379-24380	NN	denotes	C
T17010	24380-24381	HYPH	denotes	-
T17009	24381-24389	JJ	denotes	terminal
T17007	24390-24396	NN	denotes	region
T17011	24397-24398	-LRB-	denotes	(
T17013	24398-24403	NN	denotes	amino
T17014	24404-24409	NNS	denotes	acids
T17012	24410-24413	CD	denotes	463
T17015	24414-24416	IN	denotes	to
T17016	24417-24420	CD	denotes	542
T17017	24420-24421	-RRB-	denotes	)
T17018	24421-24422	.	denotes	.
R1266	T5547	T5546	prep	of,Cloning
R1267	T5548	T5549	compound	mouse,s
R1268	T5549	T5547	pobj	s,of
R1269	T5550	T5549	compound	mr,s
R1270	T5551	T5549	punct	-,s
R1271	T5553	T5554	prep	In,screened
R1272	T5555	T5553	pobj	order,In
R1273	T5556	T5557	aux	to,identify
R1274	T5557	T5555	acl	identify,order
R1275	T5558	T5559	amod	novel,genes
R1276	T5559	T5557	dobj	genes,identify
R1277	T5560	T5559	compound	mouse,genes
R1278	T5561	T5562	advmod	preferentially,expressed
R1279	T5562	T5559	acl	expressed,genes
R1280	T5563	T5562	prep	in,expressed
R1281	T5564	T5565	det	the,retina
R1282	T5565	T5563	pobj	retina,in
R1283	T5566	T5565	amod	developing,retina
R1284	T5567	T5554	punct	", ",screened
R1285	T5568	T5554	nsubj	we,screened
R1286	T5569	T5570	det	the,database
R1287	T5570	T5554	dobj	database,screened
R1288	T5571	T5572	nmod	National,Institute
R1289	T5572	T5570	nmod	Institute,database
R1290	T5573	T5572	prep	for,Institute
R1291	T5574	T5575	compound	Biotechnology,Information
R1292	T5575	T5573	pobj	Information,for
R1293	T5576	T5572	punct	(,Institute
R1294	T5577	T5572	appos	NCBI,Institute
R1295	T5578	T5570	punct	),database
R1296	T5579	T5570	punct	", ",database
R1297	T5580	T5570	appos	UniGene,database
R1298	T5581	T5554	punct	", ",screened
R1299	T5582	T5554	advcl	using,screened
R1300	T5583	T5584	compound	Digital,Display
R1301	T5584	T5582	dobj	Display,using
R1302	T5585	T5584	compound	Differential,Display
R1303	T5586	T5584	punct	(,Display
R1304	T5587	T5584	appos	DDD,Display
R1305	T5588	T5554	punct	),screened
R1306	T5589	T5554	cc	and,screened
R1307	T5590	T5554	conj	found,screened
R1308	T5591	T5592	compound	EST,fragments
R1309	T5592	T5590	dobj	fragments,found
R1310	T5593	T5594	dep	which,are
R1311	T5594	T5592	relcl	are,fragments
R1312	T5595	T5594	advmod	frequently,are
R1313	T5596	T5594	acomp	present,are
R1314	T5597	T5594	prep	in,are
R1315	T5598	T5599	nmod	mouse,libraries
R1316	T5599	T5597	pobj	libraries,in
R1317	T5600	T5599	amod	retinal,libraries
R1318	T5601	T5599	compound	cDNA,libraries
R1319	T5602	T5554	punct	.,screened
R1320	T5604	T5605	nsubj	We,found
R1321	T5606	T5607	mark	that,encodes
R1322	T5607	T5605	ccomp	encodes,found
R1323	T5608	T5609	nummod	one,clone
R1324	T5609	T5607	nsubj	clone,encodes
R1325	T5610	T5609	prep	in,clone
R1326	T5611	T5612	det	these,cDNAs
R1327	T5612	T5610	pobj	cDNAs,in
R1328	T5613	T5614	det	a,protein
R1329	T5614	T5607	dobj	protein,encodes
R1330	T5615	T5614	acl	containing,protein
R1331	T5616	T5617	det	a,domain
R1332	T5617	T5615	dobj	domain,containing
R1333	T5618	T5617	compound	SAM,domain
R1334	T5619	T5617	acl	related,domain
R1335	T5620	T5619	prep	to,related
R1336	T5621	T5620	pobj	that,to
R1337	T5622	T5621	prep	of,that
R1338	T5623	T5624	amod	polyhomeotic,protein
R1339	T5624	T5622	pobj	protein,of
R1340	T5625	T5605	punct	.,found
R1341	T5627	T5628	det	A,fragment
R1342	T5628	T5630	nsubjpass	fragment,used
R1343	T5629	T5628	compound	PCR,fragment
R1344	T5631	T5628	acl	corresponding,fragment
R1345	T5632	T5631	prep	to,corresponding
R1346	T5633	T5634	det	this,clone
R1347	T5634	T5632	pobj	clone,to
R1348	T5635	T5634	compound	mouse,clone
R1349	T5636	T5630	auxpass	was,used
R1350	T5637	T5638	aux	to,screen
R1351	T5638	T5630	advcl	screen,used
R1352	T5639	T5640	det	a,library
R1353	T5640	T5638	dobj	library,screen
R1354	T5641	T5640	nmod	mouse,library
R1355	T5642	T5643	nmod	P0,P3
R1356	T5643	T5640	nmod	P3,library
R1357	T5644	T5643	punct	-,P3
R1358	T5645	T5640	amod	retinal,library
R1359	T5646	T5640	compound	cDNA,library
R1360	T5647	T5648	aux	to,obtain
R1361	T5648	T5638	advcl	obtain,screen
R1362	T5649	T5650	det	a,clone
R1363	T5650	T5648	dobj	clone,obtain
R1364	T5651	T5652	amod	full,length
R1365	T5652	T5650	compound	length,clone
R1366	T5653	T5652	punct	-,length
R1367	T5654	T5650	compound	cDNA,clone
R1368	T5655	T5630	punct	.,used
R1369	T5657	T5658	compound	Sequence,analysis
R1370	T5658	T5659	nsubj	analysis,showed
R1371	T5660	T5661	mark	that,was
R1372	T5661	T5659	ccomp	was,showed
R1373	T5662	T5663	det	this,cDNA
R1374	T5663	T5661	nsubj	cDNA,was
R1375	T5664	T5665	det	a,gene
R1376	T5665	T5661	attr	gene,was
R1377	T5666	T5665	amod	novel,gene
R1378	T5667	T5665	acl	encoding,gene
R1379	T5668	T5669	det	a,protein
R1380	T5669	T5667	dobj	protein,encoding
R1381	T5670	T5671	compound	SAM,domain
R1382	T5671	T5672	npadvmod	domain,containing
R1383	T5672	T5669	amod	containing,protein
R1384	T5673	T5672	punct	-,containing
R1385	T5674	T5659	punct	.,showed
R1386	T5676	T5677	nsubj	We,referred
R1387	T5678	T5677	prep	to,referred
R1388	T5679	T5680	det	this,protein
R1389	T5680	T5678	pobj	protein,to
R1390	T5681	T5677	prep	as,referred
R1391	T5682	T5683	compound	mr,s
R1392	T5683	T5681	pobj	s,as
R1393	T5684	T5683	punct	-,s
R1394	T5685	T5683	punct	(,s
R1395	T5686	T5687	amod	major,protein
R1396	T5687	T5683	appos	protein,s
R1397	T5688	T5687	amod	retinal,protein
R1398	T5689	T5690	compound	SAM,domain
R1399	T5690	T5687	compound	domain,protein
R1400	T5691	T5677	punct	),referred
R1401	T5692	T5677	punct	.,referred
R1402	T5694	T5695	mark	As,shown
R1403	T5695	T5696	advcl	shown,is
R1404	T5697	T5695	prep	in,shown
R1405	T5698	T5699	compound	Fig.,1A
R1406	T5699	T5697	pobj	1A,in
R1407	T5700	T5696	punct	", ",is
R1408	T5701	T5702	det	a,codon
R1409	T5702	T5696	nsubj	codon,is
R1410	T5703	T5702	compound	translation,codon
R1411	T5704	T5702	compound	initiation,codon
R1412	T5705	T5696	acomp	present,is
R1413	T5706	T5696	prep	in,is
R1414	T5707	T5708	det	the,frame
R1415	T5708	T5706	pobj	frame,in
R1416	T5709	T5708	amod	same,frame
R1417	T5710	T5708	amod	open,frame
R1418	T5711	T5708	compound	reading,frame
R1419	T5712	T5708	prep	as,frame
R1420	T5713	T5714	det	the,domain
R1421	T5714	T5712	pobj	domain,as
R1422	T5715	T5714	compound	SAM,domain
R1423	T5716	T5696	punct	.,is
R1424	T5718	T5719	det	This,site
R1425	T5719	T5721	nsubj	site,shows
R1426	T5720	T5719	compound	initiation,site
R1427	T5722	T5721	dobj	similarity,shows
R1428	T5723	T5722	prep	to,similarity
R1429	T5724	T5725	det	the,sequence
R1430	T5725	T5723	pobj	sequence,to
R1431	T5726	T5725	compound	consensus,sequence
R1432	T5727	T5725	acl	proposed,sequence
R1433	T5728	T5727	agent	by,proposed
R1434	T5729	T5728	pobj	Kozak,by
R1435	T5730	T5731	punct	[,32
R1436	T5731	T5729	parataxis	32,Kozak
R1437	T5732	T5731	punct	],32
R1438	T5733	T5725	prep	including,sequence
R1439	T5734	T5735	det	the,presence
R1440	T5735	T5733	pobj	presence,including
R1441	T5736	T5735	prep	of,presence
R1442	T5737	T5738	det	the,purine
R1443	T5738	T5736	pobj	purine,of
R1444	T5739	T5740	advmod	highly,conserved
R1445	T5740	T5738	amod	conserved,purine
R1446	T5741	T5735	prep	at,presence
R1447	T5742	T5741	pobj	position,at
R1448	T5743	T5742	punct	-,position
R1449	T5744	T5742	nummod	3,position
R1450	T5745	T5721	punct	.,shows
R1451	T5747	T5748	det	The,codon
R1452	T5748	T5750	nsubjpass	codon,indicated
R1453	T5749	T5748	compound	stop,codon
R1454	T5751	T5748	prep	of,codon
R1455	T5752	T5753	det	the,protein
R1456	T5753	T5751	pobj	protein,of
R1457	T5754	T5753	amod	predicted,protein
R1458	T5755	T5756	compound	mr,s
R1459	T5756	T5753	compound	s,protein
R1460	T5757	T5756	punct	-,s
R1461	T5758	T5750	auxpass	is,indicated
R1462	T5759	T5750	advmod	also,indicated
R1463	T5760	T5750	prep	in,indicated
R1464	T5761	T5762	compound	Fig.,1A
R1465	T5762	T5760	pobj	1A,in
R1466	T5763	T5750	punct	.,indicated
R1467	T5765	T5766	det	The,sequence
R1468	T5766	T5769	nsubj	sequence,exhibits
R1469	T5767	T5768	compound	amino,acid
R1470	T5768	T5766	compound	acid,sequence
R1471	T5770	T5766	prep	of,sequence
R1472	T5771	T5772	det	the,domain
R1473	T5772	T5770	pobj	domain,of
R1474	T5773	T5772	compound	SAM,domain
R1475	T5774	T5772	prep	of,domain
R1476	T5775	T5776	compound	mr,s
R1477	T5776	T5778	compound	s,protein
R1478	T5777	T5776	punct	-,s
R1479	T5778	T5774	pobj	protein,of
R1480	T5779	T5780	punct	(,1A
R1481	T5780	T5766	parataxis	1A,sequence
R1482	T5781	T5780	compound	Fig.,1A
R1483	T5782	T5780	punct	", ",1A
R1484	T5783	T5784	amod	boxed,sequence
R1485	T5784	T5780	appos	sequence,1A
R1486	T5785	T5780	punct	),1A
R1487	T5786	T5769	dobj	homology,exhibits
R1488	T5787	T5786	prep	with,homology
R1489	T5788	T5789	compound	SAM,domains
R1490	T5789	T5787	pobj	domains,with
R1491	T5790	T5789	prep	of,domains
R1492	T5791	T5790	pobj	EphB2,of
R1493	T5792	T5791	punct	", ",EphB2
R1494	T5793	T5791	conj	EphA4,EphB2
R1495	T5794	T5793	punct	", ",EphA4
R1496	T5795	T5793	conj	MPH1,EphA4
R1497	T5796	T5795	punct	", ",MPH1
R1498	T5797	T5795	conj	TEL,MPH1
R1499	T5798	T5797	cc	and,TEL
R1500	T5799	T5797	conj	Smaug,TEL
R1501	T5800	T5801	punct	(,1B
R1502	T5801	T5769	parataxis	1B,exhibits
R1503	T5802	T5801	compound	Fig.,1B
R1504	T5803	T5801	punct	),1B
R1505	T5804	T5769	punct	.,exhibits
R1506	T5806	T5807	prep	By,related
R1507	T5808	T5809	amod	phylogenetic,analysis
R1508	T5809	T5806	pobj	analysis,By
R1509	T5810	T5807	punct	", ",related
R1510	T5811	T5812	det	the,domain
R1511	T5812	T5807	nsubjpass	domain,related
R1512	T5813	T5812	compound	SAM,domain
R1513	T5814	T5812	prep	of,domain
R1514	T5815	T5816	compound	mr,s
R1515	T5816	T5814	pobj	s,of
R1516	T5817	T5816	punct	-,s
R1517	T5818	T5807	auxpass	is,related
R1518	T5819	T5820	advmod	most,closely
R1519	T5820	T5807	advmod	closely,related
R1520	T5821	T5807	prep	to,related
R1521	T5822	T5821	pobj	that,to
R1522	T5823	T5822	prep	of,that
R1523	T5824	T5825	compound	Mph1,Rae28
R1524	T5825	T5823	pobj	Rae28,of
R1525	T5826	T5825	punct	/,Rae28
R1526	T5827	T5825	punct	", ",Rae28
R1527	T5828	T5829	det	a,homolog
R1528	T5829	T5825	appos	homolog,Rae28
R1529	T5830	T5829	compound	mouse,homolog
R1530	T5831	T5829	prep	of,homolog
R1531	T5832	T5831	pobj	ph,of
R1532	T5833	T5834	punct	(,1C
R1533	T5834	T5807	parataxis	1C,related
R1534	T5835	T5834	compound	Fig.,1C
R1535	T5836	T5834	punct	),1C
R1536	T5837	T5807	punct	.,related
R1537	T5839	T5840	compound	Mouse,protein
R1538	T5840	T5844	nsubjpass	protein,conserved
R1539	T5841	T5842	compound	mr,s
R1540	T5842	T5840	compound	s,protein
R1541	T5843	T5842	punct	-,s
R1542	T5845	T5844	auxpass	is,conserved
R1543	T5846	T5844	prep	in,conserved
R1544	T5847	T5846	pobj	rat,in
R1545	T5848	T5847	punct	", ",rat
R1546	T5849	T5847	amod	human,rat
R1547	T5850	T5847	punct	", ",rat
R1548	T5851	T5847	conj	chick,rat
R1549	T5852	T5851	cc	and,chick
R1550	T5853	T5851	conj	zebrafish,chick
R1551	T5854	T5847	punct	", ",rat
R1552	T5855	T5856	dep	which,display
R1553	T5856	T5847	relcl	display,rat
R1554	T5857	T5858	nummod	91,%
R1555	T5858	T5859	nmod	%,identity
R1556	T5859	T5856	dobj	identity,display
R1557	T5860	T5858	punct	", ",%
R1558	T5861	T5862	nummod	70,%
R1559	T5862	T5858	conj	%,%
R1560	T5863	T5862	punct	", ",%
R1561	T5864	T5865	nummod	36,%
R1562	T5865	T5862	conj	%,%
R1563	T5866	T5865	cc	and,%
R1564	T5867	T5868	nummod	26,%
R1565	T5868	T5865	conj	%,%
R1566	T5869	T5859	prep	with,identity
R1567	T5870	T5871	compound	mouse,s
R1568	T5871	T5869	pobj	s,with
R1569	T5872	T5871	compound	mr,s
R1570	T5873	T5871	punct	-,s
R1571	T5874	T5856	punct	", ",display
R1572	T5875	T5856	advmod	respectively,display
R1573	T5876	T5877	punct	(,1D
R1574	T5877	T5844	parataxis	1D,conserved
R1575	T5878	T5877	compound	Fig.,1D
R1576	T5879	T5877	punct	),1D
R1577	T5880	T5844	punct	.,conserved
R1578	T5882	T5883	det	The,domains
R1579	T5883	T5885	nsubjpass	domains,conserved
R1580	T5884	T5883	compound	SAM,domains
R1581	T5886	T5883	prep	of,domains
R1582	T5887	T5888	nmod	rat,protein
R1583	T5888	T5886	pobj	protein,of
R1584	T5889	T5887	punct	", ",rat
R1585	T5890	T5887	amod	human,rat
R1586	T5891	T5887	punct	", ",rat
R1587	T5892	T5887	conj	chick,rat
R1588	T5893	T5892	cc	and,chick
R1589	T5894	T5892	conj	zebrafish,chick
R1590	T5895	T5896	compound	mr,s
R1591	T5896	T5888	compound	s,protein
R1592	T5897	T5896	punct	-,s
R1593	T5898	T5885	auxpass	are,conserved
R1594	T5899	T5885	advmod	highly,conserved
R1595	T5900	T5885	cc	and,conserved
R1596	T5901	T5885	conj	display,conserved
R1597	T5902	T5903	nummod	96,%
R1598	T5903	T5904	nmod	%,identity
R1599	T5904	T5901	dobj	identity,display
R1600	T5905	T5903	punct	", ",%
R1601	T5906	T5907	nummod	90,%
R1602	T5907	T5903	conj	%,%
R1603	T5908	T5907	punct	", ",%
R1604	T5909	T5910	nummod	76,%
R1605	T5910	T5907	conj	%,%
R1606	T5911	T5910	cc	and,%
R1607	T5912	T5913	nummod	72,%
R1608	T5913	T5910	conj	%,%
R1609	T5914	T5904	prep	with,identity
R1610	T5915	T5916	det	the,domain
R1611	T5916	T5914	pobj	domain,with
R1612	T5917	T5916	compound	SAM,domain
R1613	T5918	T5916	prep	of,domain
R1614	T5919	T5920	compound	mouse,protein
R1615	T5920	T5918	pobj	protein,of
R1616	T5921	T5922	compound	mr,s
R1617	T5922	T5920	compound	s,protein
R1618	T5923	T5922	punct	-,s
R1619	T5924	T5885	punct	.,conserved
R1620	T5926	T5927	det	The,localizations
R1621	T5927	T5929	nsubjpass	localizations,determined
R1622	T5928	T5927	amod	chromosomal,localizations
R1623	T5930	T5927	prep	of,localizations
R1624	T5931	T5932	nmod	mouse,genes
R1625	T5932	T5930	pobj	genes,of
R1626	T5933	T5931	cc	and,mouse
R1627	T5934	T5931	conj	human,mouse
R1628	T5935	T5936	compound	mr,s
R1629	T5936	T5932	compound	s,genes
R1630	T5937	T5936	punct	-,s
R1631	T5938	T5929	auxpass	were,determined
R1632	T5939	T5929	prep	by,determined
R1633	T5940	T5939	pcomp	searching,by
R1634	T5941	T5942	det	the,databases
R1635	T5942	T5940	dobj	databases,searching
R1636	T5943	T5942	nmod	mouse,databases
R1637	T5944	T5943	cc	and,mouse
R1638	T5945	T5943	conj	human,mouse
R1639	T5946	T5942	compound	genome,databases
R1640	T5947	T5948	punct	(,NCBI
R1641	T5948	T5942	parataxis	NCBI,databases
R1642	T5949	T5948	punct	),NCBI
R1643	T5950	T5940	punct	", ",searching
R1644	T5951	T5940	advmod	respectively,searching
R1645	T5952	T5929	punct	.,determined
R1646	T5954	T5955	compound	Mouse,s
R1647	T5955	T5958	nsubjpass	s,mapped
R1648	T5956	T5955	compound	mr,s
R1649	T5957	T5955	punct	-,s
R1650	T5959	T5958	auxpass	is,mapped
R1651	T5960	T5958	prep	to,mapped
R1652	T5961	T5962	compound	chromosome,4E2
R1653	T5962	T5960	pobj	4E2,to
R1654	T5963	T5958	punct	", ",mapped
R1655	T5964	T5958	cc	and,mapped
R1656	T5965	T5966	amod	human,S
R1657	T5966	T5969	nsubjpass	S,mapped
R1658	T5967	T5966	compound	MR,S
R1659	T5968	T5966	punct	-,S
R1660	T5969	T5958	conj	mapped,mapped
R1661	T5970	T5969	auxpass	is,mapped
R1662	T5971	T5969	prep	to,mapped
R1663	T5972	T5973	compound	chromosome,1p36.33
R1664	T5973	T5971	pobj	1p36.33,to
R1665	T5974	T5969	punct	.,mapped
R1666	T5976	T5977	nsubj	LCA,is
R1667	T5978	T5979	det	the,cause
R1668	T5979	T5977	attr	cause,is
R1669	T5980	T5981	advmod	most,common
R1670	T5981	T5979	amod	common,cause
R1671	T5982	T5979	prep	of,cause
R1672	T5983	T5984	amod	inherited,blindness
R1673	T5984	T5982	pobj	blindness,of
R1674	T5985	T5984	compound	childhood,blindness
R1675	T5986	T5977	punct	.,is
R1676	T5988	T5989	amod	Human,S
R1677	T5989	T5992	nsubj	S,maps
R1678	T5990	T5989	compound	MR,S
R1679	T5991	T5989	punct	-,S
R1680	T5993	T5992	prep	in,maps
R1681	T5994	T5995	det	the,region
R1682	T5995	T5993	pobj	region,in
R1683	T5996	T5995	compound	vicinity,region
R1684	T5997	T5995	prep	of,region
R1685	T5998	T5999	det	the,LCA9
R1686	T5999	T5997	pobj	LCA9,of
R1687	T6000	T5999	punct	", ",LCA9
R1688	T6001	T6002	advmod	recently,identified
R1689	T6002	T5999	acl	identified,LCA9
R1690	T6003	T6002	prep	as,identified
R1691	T6004	T6005	det	a,locus
R1692	T6005	T6003	pobj	locus,as
R1693	T6006	T6005	amod	new,locus
R1694	T6007	T6005	prep	for,locus
R1695	T6008	T6007	pobj	LCA,for
R1696	T6009	T6010	punct	[,33
R1697	T6010	T5992	parataxis	33,maps
R1698	T6011	T6010	punct	],33
R1699	T6012	T5992	punct	.,maps
R1702	T7366	T7365	prep	of,Expression
R1703	T7367	T7368	compound	mr,s
R1704	T7368	T7366	pobj	s,of
R1705	T7369	T7368	punct	-,s
R1706	T7370	T7365	prep	in,Expression
R1707	T7371	T7372	det	the,retina
R1708	T7372	T7370	pobj	retina,in
R1709	T7373	T7372	amod	developing,retina
R1710	T7374	T7372	cc	and,retina
R1711	T7375	T7376	det	the,gland
R1712	T7376	T7372	conj	gland,retina
R1713	T7377	T7376	amod	pineal,gland
R1714	T7379	T7380	aux	To,investigate
R1715	T7380	T7381	advcl	investigate,performed
R1716	T7382	T7383	compound	mr,s
R1717	T7383	T7385	compound	s,expression
R1718	T7384	T7383	punct	-,s
R1719	T7385	T7380	dobj	expression,investigate
R1720	T7386	T7381	punct	", ",performed
R1721	T7387	T7381	nsubj	we,performed
R1722	T7388	T7381	advmod	first,performed
R1723	T7389	T7390	amod	whole,mount
R1724	T7390	T7391	nmod	mount,hybridization
R1725	T7391	T7381	dobj	hybridization,performed
R1726	T7392	T7393	advmod	in,situ
R1727	T7393	T7391	amod	situ,hybridization
R1728	T7394	T7381	prep	in,performed
R1729	T7395	T7396	compound	mouse,embryos
R1730	T7396	T7394	pobj	embryos,in
R1731	T7397	T7381	punct	.,performed
R1732	T7399	T7400	det	No,signal
R1733	T7400	T7402	nsubjpass	signal,detected
R1734	T7401	T7400	compound	hybridization,signal
R1735	T7403	T7402	auxpass	was,detected
R1736	T7404	T7402	prep	at,detected
R1737	T7405	T7404	pobj	E9.5,at
R1738	T7406	T7405	punct	", ",E9.5
R1739	T7407	T7405	conj	E10.5,E9.5
R1740	T7408	T7407	cc	and,E10.5
R1741	T7409	T7407	conj	E11.5,E10.5
R1742	T7410	T7402	prep	with,detected
R1743	T7411	T7412	det	an,probe
R1744	T7412	T7410	pobj	probe,with
R1745	T7413	T7414	compound	mr,s
R1746	T7414	T7412	compound	s,probe
R1747	T7415	T7414	punct	-,s
R1748	T7416	T7417	punct	(,shown
R1749	T7417	T7402	parataxis	shown,detected
R1750	T7418	T7417	nsubj	data,shown
R1751	T7419	T7417	neg	not,shown
R1752	T7420	T7417	punct	),shown
R1753	T7421	T7402	punct	.,detected
R1754	T7423	T7424	nsubj	We,investigated
R1755	T7425	T7424	advmod	then,investigated
R1756	T7426	T7427	det	the,expression
R1757	T7427	T7424	dobj	expression,investigated
R1758	T7428	T7427	prep	of,expression
R1759	T7429	T7430	det	the,gene
R1760	T7430	T7428	pobj	gene,of
R1761	T7431	T7432	compound	mr,s
R1762	T7432	T7430	compound	s,gene
R1763	T7433	T7432	punct	-,s
R1764	T7434	T7424	prep	in,investigated
R1765	T7435	T7436	det	the,retina
R1766	T7436	T7434	pobj	retina,in
R1767	T7437	T7436	amod	developing,retina
R1768	T7438	T7424	prep	by,investigated
R1769	T7439	T7440	nmod	section,hybridization
R1770	T7440	T7438	pobj	hybridization,by
R1771	T7441	T7442	advmod	in,situ
R1772	T7442	T7440	amod	situ,hybridization
R1773	T7443	T7444	punct	(,1A
R1774	T7444	T7424	parataxis	1A,investigated
R1775	T7445	T7444	compound	Fig.,1A
R1776	T7446	T7447	punct	–,G
R1777	T7447	T7444	prep	G,1A
R1778	T7448	T7444	punct	),1A
R1779	T7449	T7424	punct	.,investigated
R1780	T7451	T7452	det	No,signal
R1781	T7452	T7454	nsubjpass	signal,detected
R1782	T7453	T7452	amod	significant,signal
R1783	T7455	T7454	auxpass	was,detected
R1784	T7456	T7454	prep	in,detected
R1785	T7457	T7458	det	the,retina
R1786	T7458	T7456	pobj	retina,in
R1787	T7459	T7458	amod	developing,retina
R1788	T7460	T7454	prep	at,detected
R1789	T7461	T7460	pobj	E13,at
R1790	T7462	T7463	punct	(,2A
R1791	T7463	T7454	parataxis	2A,detected
R1792	T7464	T7463	compound	Fig.,2A
R1793	T7465	T7463	punct	),2A
R1794	T7466	T7454	punct	.,detected
R1795	T7468	T7469	det	A,signal
R1796	T7469	T7471	nsubjpass	signal,detected
R1797	T7470	T7469	amod	weak,signal
R1798	T7472	T7471	auxpass	was,detected
R1799	T7473	T7471	advmod	initially,detected
R1800	T7474	T7471	prep	in,detected
R1801	T7475	T7476	det	the,aspect
R1802	T7476	T7474	pobj	aspect,in
R1803	T7477	T7476	amod	outer,aspect
R1804	T7478	T7476	prep	of,aspect
R1805	T7479	T7480	det	the,layer
R1806	T7480	T7478	pobj	layer,of
R1807	T7481	T7480	amod	neuroblastic,layer
R1808	T7482	T7480	punct	(,layer
R1809	T7483	T7480	appos	NBL,layer
R1810	T7484	T7480	punct	),layer
R1811	T7485	T7480	punct	", ",layer
R1812	T7486	T7487	det	a,layer
R1813	T7487	T7480	appos	layer,layer
R1814	T7488	T7487	amod	presumptive,layer
R1815	T7489	T7487	compound	photoreceptor,layer
R1816	T7490	T7471	prep	at,detected
R1817	T7491	T7490	pobj	E18,at
R1818	T7492	T7493	punct	(,2B
R1819	T7493	T7471	parataxis	2B,detected
R1820	T7494	T7493	compound	Fig.,2B
R1821	T7495	T7493	punct	", ",2B
R1822	T7496	T7493	appos	arrow,2B
R1823	T7497	T7493	punct	),2B
R1824	T7498	T7471	punct	.,detected
R1825	T7500	T7501	prep	At,detected
R1826	T7502	T7500	pobj	P3,At
R1827	T7503	T7501	punct	", ",detected
R1828	T7504	T7505	det	an,transcript
R1829	T7505	T7501	nsubjpass	transcript,detected
R1830	T7506	T7507	compound	mr,s
R1831	T7507	T7505	compound	s,transcript
R1832	T7508	T7507	punct	-,s
R1833	T7509	T7501	auxpass	was,detected
R1834	T7510	T7501	advmod	clearly,detected
R1835	T7511	T7501	prep	in,detected
R1836	T7512	T7513	det	the,layer
R1837	T7513	T7511	pobj	layer,in
R1838	T7514	T7513	amod	developing,layer
R1839	T7515	T7513	compound	photoreceptor,layer
R1840	T7516	T7517	punct	(,2E
R1841	T7517	T7501	parataxis	2E,detected
R1842	T7518	T7517	compound	Fig.,2E
R1843	T7519	T7517	punct	),2E
R1844	T7520	T7501	punct	.,detected
R1845	T7522	T7523	prep	At,showed
R1846	T7524	T7522	pobj	P6,At
R1847	T7525	T7523	punct	", ",showed
R1848	T7526	T7527	compound	mr,s
R1849	T7527	T7523	nsubj	s,showed
R1850	T7528	T7527	punct	-,s
R1851	T7529	T7530	compound	peak,expression
R1852	T7530	T7523	dobj	expression,showed
R1853	T7531	T7523	prep	in,showed
R1854	T7532	T7533	det	the,layer
R1855	T7533	T7531	pobj	layer,in
R1856	T7534	T7533	compound	photoreceptor,layer
R1857	T7535	T7536	punct	(,2F
R1858	T7536	T7523	parataxis	2F,showed
R1859	T7537	T7536	compound	Fig.,2F
R1860	T7538	T7536	punct	),2F
R1861	T7539	T7523	punct	.,showed
R1862	T7541	T7542	det	This,pattern
R1863	T7542	T7543	nsubj	pattern,correlates
R1864	T7544	T7543	prep	with,correlates
R1865	T7545	T7546	det	the,increase
R1866	T7546	T7544	pobj	increase,with
R1867	T7547	T7546	amod	rapid,increase
R1868	T7548	T7546	prep	in,increase
R1869	T7549	T7548	pobj	cells,in
R1870	T7550	T7549	acl	expressing,cells
R1871	T7551	T7550	dobj	rhodopsin,expressing
R1872	T7552	T7551	cc	and,rhodopsin
R1873	T7553	T7554	amod	other,genes
R1874	T7554	T7551	conj	genes,rhodopsin
R1875	T7555	T7554	compound	phototransduction,genes
R1876	T7556	T7546	prep	between,increase
R1877	T7557	T7556	pobj	P6,between
R1878	T7558	T7559	punct	-,P8
R1879	T7559	T7557	prep	P8,P6
R1880	T7560	T7561	punct	[,34
R1881	T7561	T7543	parataxis	34,correlates
R1882	T7562	T7563	punct	-,37
R1883	T7563	T7561	prep	37,34
R1884	T7564	T7561	punct	],34
R1885	T7565	T7543	punct	.,correlates
R1886	T7567	T7568	prep	At,reduced
R1887	T7569	T7567	pobj	P9,At
R1888	T7570	T7568	punct	", ",reduced
R1889	T7571	T7572	det	the,intensity
R1890	T7572	T7568	nsubjpass	intensity,reduced
R1891	T7573	T7572	prep	of,intensity
R1892	T7574	T7575	det	the,signal
R1893	T7575	T7573	pobj	signal,of
R1894	T7576	T7577	compound	mr,s
R1895	T7577	T7575	compound	s,signal
R1896	T7578	T7577	punct	-,s
R1897	T7579	T7568	auxpass	was,reduced
R1898	T7580	T7568	advmod	significantly,reduced
R1899	T7581	T7568	cc	but,reduced
R1900	T7582	T7583	auxpass	was,localized
R1901	T7583	T7568	conj	localized,reduced
R1902	T7584	T7583	prep	to,localized
R1903	T7585	T7586	det	the,layer
R1904	T7586	T7584	pobj	layer,to
R1905	T7587	T7586	amod	outer,layer
R1906	T7588	T7586	amod	nuclear,layer
R1907	T7589	T7586	punct	(,layer
R1908	T7590	T7586	appos	ONL,layer
R1909	T7591	T7583	punct	),localized
R1910	T7592	T7593	punct	(,shown
R1911	T7593	T7583	parataxis	shown,localized
R1912	T7594	T7593	nsubj	data,shown
R1913	T7595	T7593	neg	not,shown
R1914	T7596	T7593	punct	),shown
R1915	T7597	T7568	punct	.,reduced
R1916	T7599	T7600	compound	Faint,expression
R1917	T7600	T7601	nsubjpass	expression,observed
R1918	T7602	T7600	prep	of,expression
R1919	T7603	T7604	compound	mr,s
R1920	T7604	T7606	compound	s,mRNA
R1921	T7605	T7604	punct	-,s
R1922	T7606	T7602	pobj	mRNA,of
R1923	T7607	T7601	auxpass	was,observed
R1924	T7608	T7601	prep	in,observed
R1925	T7609	T7610	amod	mature,photoreceptors
R1926	T7610	T7608	pobj	photoreceptors,in
R1927	T7611	T7601	prep	in,observed
R1928	T7612	T7613	det	the,retina
R1929	T7613	T7611	pobj	retina,in
R1930	T7614	T7613	amod	adult,retina
R1931	T7615	T7616	punct	(,2G
R1932	T7616	T7601	parataxis	2G,observed
R1933	T7617	T7616	compound	Fig.,2G
R1934	T7618	T7616	punct	),2G
R1935	T7619	T7601	punct	.,observed
R1936	T7628	T7630	nsubjpass	in,e
R1937	T7632	T7633	det	he,ti
R1938	T7634	T7630	conj	s,e
R1939	T7635	T7634	nsubj	s,s
R1940	T7637	T7636	amod	u,e
R1941	T7640	T7639	compound	s,pe
R1942	T7645	T7644	prep	f,i
R1943	T7646	T7645	pobj	i,f
R1944	T7652	T7651	prep	of,y
R1945	T7654	T7653	pobj	r,m
R1946	T7657	T7659	compound	s,expression
R1947	T7658	T7657	punct	-,s
R1948	T7660	T7656	punct	", ",examined
R1949	T7661	T7662	det	the,expression
R1950	T7662	T7656	nsubjpass	expression,examined
R1951	T7663	T7662	prep	of,expression
R1952	T7664	T7665	det	the,gene
R1953	T7665	T7663	pobj	gene,of
R1954	T7666	T7667	compound	mr,s
R1955	T7667	T7665	compound	s,gene
R1956	T7668	T7667	punct	-,s
R1957	T7669	T7662	prep	in,expression
R1958	T7670	T7671	amod	various,tissues
R1959	T7671	T7669	pobj	tissues,in
R1960	T7672	T7671	amod	adult,tissues
R1961	T7673	T7656	auxpass	was,examined
R1962	T7674	T7656	prep	by,examined
R1963	T7675	T7676	compound	Northern,hybridization
R1964	T7676	T7674	pobj	hybridization,by
R1965	T7677	T7678	punct	(,2H
R1966	T7678	T7656	parataxis	2H,examined
R1967	T7679	T7678	compound	Fig.,2H
R1968	T7680	T7678	punct	),2H
R1969	T7681	T7656	punct	.,examined
R1970	T7683	T7684	prep	As,used
R1971	T7685	T7686	det	a,control
R1972	T7686	T7683	pobj	control,As
R1973	T7687	T7684	punct	", ",used
R1974	T7688	T7689	compound	P7,RNA
R1975	T7689	T7684	nsubjpass	RNA,used
R1976	T7690	T7689	compound	retinal,RNA
R1977	T7691	T7684	auxpass	was,used
R1978	T7692	T7684	punct	.,used
R1979	T7694	T7695	nummod	Four,bands
R1980	T7695	T7696	nsubjpass	bands,detected
R1981	T7697	T7695	acl	corresponding,bands
R1982	T7698	T7697	prep	to,corresponding
R1983	T7699	T7700	nummod	7.2,kb
R1984	T7700	T7698	pobj	kb,to
R1985	T7701	T7700	punct	-,kb
R1986	T7702	T7700	punct	", ",kb
R1987	T7703	T7704	nummod	4.0,kb
R1988	T7704	T7700	conj	kb,kb
R1989	T7705	T7704	punct	-,kb
R1990	T7706	T7704	punct	", ",kb
R1991	T7707	T7708	nummod	2.5,kb
R1992	T7708	T7704	conj	kb,kb
R1993	T7709	T7708	punct	-,kb
R1994	T7710	T7708	cc	and,kb
R1995	T7711	T7712	nummod	2.2,kb
R1996	T7712	T7708	conj	kb,kb
R1997	T7713	T7712	punct	-,kb
R1998	T7714	T7696	auxpass	were,detected
R1999	T7715	T7696	prep	in,detected
R2000	T7716	T7717	compound	P7,retina
R2001	T7717	T7715	pobj	retina,in
R2002	T7718	T7696	punct	.,detected
R2003	T7720	T7721	det	The,band
R2004	T7721	T7725	nsubj	band,corresponds
R2005	T7722	T7723	nummod	2.2,kb
R2006	T7723	T7721	compound	kb,band
R2007	T7724	T7723	punct	-,kb
R2008	T7726	T7725	prep	to,corresponds
R2009	T7727	T7728	det	the,cDNA
R2010	T7728	T7726	pobj	cDNA,to
R2011	T7729	T7728	acl	characterized,cDNA
R2012	T7730	T7729	prep	in,characterized
R2013	T7731	T7732	det	this,study
R2014	T7732	T7730	pobj	study,in
R2015	T7733	T7725	punct	.,corresponds
R2016	T7735	T7736	det	The,bands
R2017	T7736	T7738	nsubjpass	bands,characterized
R2018	T7737	T7736	amod	larger,bands
R2019	T7739	T7736	punct	", ",bands
R2020	T7740	T7741	advmod	possibly,transcripts
R2021	T7741	T7736	appos	transcripts,bands
R2022	T7742	T7743	amod	alternative,spliced
R2023	T7743	T7741	amod	spliced,transcripts
R2024	T7744	T7738	punct	", ",characterized
R2025	T7745	T7738	aux	have,characterized
R2026	T7746	T7738	neg	not,characterized
R2027	T7747	T7738	advmod	yet,characterized
R2028	T7748	T7738	auxpass	been,characterized
R2029	T7749	T7738	punct	.,characterized
R2030	T7751	T7752	det	The,probe
R2031	T7752	T7756	nsubj	probe,detect
R2032	T7753	T7754	compound	mr,s
R2033	T7754	T7752	compound	s,probe
R2034	T7755	T7754	punct	-,s
R2035	T7757	T7756	aux	did,detect
R2036	T7758	T7756	neg	not,detect
R2037	T7759	T7760	det	a,band
R2038	T7760	T7756	dobj	band,detect
R2039	T7761	T7756	prep	in,detect
R2040	T7762	T7763	det	the,tissues
R2041	T7763	T7761	pobj	tissues,in
R2042	T7764	T7763	amod	adult,tissues
R2043	T7765	T7763	acl	examined,tissues
R2044	T7766	T7756	punct	", ",detect
R2045	T7767	T7756	advcl	indicating,detect
R2046	T7768	T7769	mark	that,express
R2047	T7769	T7767	ccomp	express,indicating
R2048	T7770	T7771	det	these,tissues
R2049	T7771	T7769	nsubj	tissues,express
R2050	T7772	T7769	aux	do,express
R2051	T7773	T7769	neg	not,express
R2052	T7774	T7775	compound	mr,s
R2053	T7775	T7769	dobj	s,express
R2054	T7776	T7775	punct	-,s
R2055	T7777	T7769	prep	at,express
R2056	T7778	T7779	det	a,level
R2057	T7779	T7777	pobj	level,at
R2058	T7780	T7779	amod	comparable,level
R2059	T7781	T7780	prep	to,comparable
R2060	T7782	T7781	pobj	that,to
R2061	T7783	T7782	prep	of,that
R2062	T7784	T7785	det	the,retina
R2063	T7785	T7783	pobj	retina,of
R2064	T7786	T7785	amod	developing,retina
R2065	T7787	T7756	punct	.,detect
R2066	T7789	T7790	amod	Previous,reports
R2067	T7790	T7791	nsubj	reports,revealed
R2068	T7792	T7793	mark	that,expressed
R2069	T7793	T7791	ccomp	expressed,revealed
R2070	T7794	T7795	amod	many,genes
R2071	T7795	T7793	nsubjpass	genes,expressed
R2072	T7796	T7797	npadvmod	photoreceptor,specific
R2073	T7797	T7795	amod	specific,genes
R2074	T7798	T7797	punct	-,specific
R2075	T7799	T7793	auxpass	are,expressed
R2076	T7800	T7793	advmod	also,expressed
R2077	T7801	T7793	prep	in,expressed
R2078	T7802	T7803	det	the,gland
R2079	T7803	T7801	pobj	gland,in
R2080	T7804	T7803	amod	pineal,gland
R2081	T7805	T7806	punct	[,38
R2082	T7806	T7791	parataxis	38,revealed
R2083	T7807	T7806	punct	],38
R2084	T7808	T7791	punct	.,revealed
R2085	T7810	T7811	nsubj	We,examined
R2086	T7812	T7813	det	the,expression
R2087	T7813	T7811	dobj	expression,examined
R2088	T7814	T7813	prep	of,expression
R2089	T7815	T7816	compound	mr,s
R2090	T7816	T7818	compound	s,transcripts
R2091	T7817	T7816	punct	-,s
R2092	T7818	T7814	pobj	transcripts,of
R2093	T7819	T7813	prep	in,expression
R2094	T7820	T7821	det	the,embryo
R2095	T7821	T7819	pobj	embryo,in
R2096	T7822	T7821	amod	whole,embryo
R2097	T7823	T7821	punct	", ",embryo
R2098	T7824	T7825	amod	whole,body
R2099	T7825	T7821	conj	body,embryo
R2100	T7826	T7825	punct	", ",body
R2101	T7827	T7825	conj	retina,body
R2102	T7828	T7827	punct	", ",retina
R2103	T7829	T7830	amod	pineal,gland
R2104	T7830	T7827	conj	gland,retina
R2105	T7831	T7830	punct	", ",gland
R2106	T7832	T7830	conj	brain,gland
R2107	T7833	T7832	punct	", ",brain
R2108	T7834	T7832	conj	liver,brain
R2109	T7835	T7834	cc	and,liver
R2110	T7836	T7837	amod	other,organs
R2111	T7837	T7834	conj	organs,liver
R2112	T7838	T7811	prep	at,examined
R2113	T7839	T7840	amod	various,stages
R2114	T7840	T7838	pobj	stages,at
R2115	T7841	T7811	prep	by,examined
R2116	T7842	T7843	compound	RT,PCR
R2117	T7843	T7841	pobj	PCR,by
R2118	T7844	T7843	punct	-,PCR
R2119	T7845	T7846	punct	(,2I
R2120	T7846	T7811	parataxis	2I,examined
R2121	T7847	T7846	compound	Fig.,2I
R2122	T7848	T7846	cc	and,2I
R2123	T7849	T7850	nsubj	data,shown
R2124	T7850	T7846	conj	shown,2I
R2125	T7851	T7850	neg	not,shown
R2126	T7852	T7846	punct	),2I
R2127	T7853	T7811	punct	.,examined
R2128	T7855	T7856	nsubj	We,amplified
R2129	T7857	T7858	compound	PCR,fragments
R2130	T7858	T7856	dobj	fragments,amplified
R2131	T7859	T7858	prep	of,fragments
R2132	T7860	T7861	nummod	292,bp
R2133	T7861	T7859	pobj	bp,of
R2134	T7862	T7861	cc	and,bp
R2135	T7863	T7864	nummod	452,bp
R2136	T7864	T7861	conj	bp,bp
R2137	T7865	T7856	prep	with,amplified
R2138	T7866	T7867	compound	primer,pairs
R2139	T7867	T7865	pobj	pairs,with
R2140	T7868	T7867	prep	for,pairs
R2141	T7869	T7868	pobj	genes,for
R2142	T7870	T7869	acl	encoding,genes
R2143	T7871	T7872	compound	mouse,s
R2144	T7872	T7870	dobj	s,encoding
R2145	T7873	T7872	compound	mr,s
R2146	T7874	T7872	punct	-,s
R2147	T7875	T7872	cc	and,s
R2148	T7876	T7872	conj	G3PDH,s
R2149	T7877	T7870	punct	", ",encoding
R2150	T7878	T7870	advmod	respectively,encoding
R2151	T7879	T7856	punct	.,amplified
R2152	T7881	T7882	prep	In,detected
R2153	T7883	T7884	nmod	E13,embryo
R2154	T7884	T7881	pobj	embryo,In
R2155	T7885	T7884	amod	whole,embryo
R2156	T7886	T7884	cc	and,embryo
R2157	T7887	T7888	nmod	P0,body
R2158	T7888	T7884	conj	body,embryo
R2159	T7889	T7888	amod	whole,body
R2160	T7890	T7888	punct	(,body
R2161	T7891	T7888	prep	except,body
R2162	T7892	T7891	prep	for,except
R2163	T7893	T7894	det	the,eye
R2164	T7894	T7892	pobj	eye,for
R2165	T7895	T7882	punct	),detected
R2166	T7896	T7882	punct	", ",detected
R2167	T7897	T7898	det	no,signal
R2168	T7898	T7882	nsubjpass	signal,detected
R2169	T7899	T7900	compound	mr,s
R2170	T7900	T7898	compound	s,signal
R2171	T7901	T7900	punct	-,s
R2172	T7902	T7882	auxpass	was,detected
R2173	T7903	T7882	punct	.,detected
R2174	T7905	T7906	mark	As,expected
R2175	T7906	T7907	advcl	expected,observed
R2176	T7908	T7907	punct	", ",observed
R2177	T7909	T7907	nsubj	we,observed
R2178	T7910	T7911	mark	that,expressed
R2179	T7911	T7907	ccomp	expressed,observed
R2180	T7912	T7913	compound	mr,s
R2181	T7913	T7911	nsubjpass	s,expressed
R2182	T7914	T7913	punct	-,s
R2183	T7915	T7911	auxpass	is,expressed
R2184	T7916	T7911	prep	in,expressed
R2185	T7917	T7918	det	the,gland
R2186	T7918	T7916	pobj	gland,in
R2187	T7919	T7918	nmod	P7,gland
R2188	T7920	T7919	cc	and,P7
R2189	T7921	T7919	conj	adult,P7
R2190	T7922	T7918	amod	pineal,gland
R2191	T7923	T7907	punct	.,observed
R2192	T7925	T7926	prep	In,detected
R2193	T7927	T7928	det	the,brain
R2194	T7928	T7925	pobj	brain,In
R2195	T7929	T7928	nmod	P7,brain
R2196	T7930	T7929	cc	and,P7
R2197	T7931	T7929	conj	adult,P7
R2198	T7932	T7928	punct	", ",brain
R2199	T7933	T7928	conj	liver,brain
R2200	T7934	T7933	cc	and,liver
R2201	T7935	T7936	amod	several,organs
R2202	T7936	T7933	conj	organs,liver
R2203	T7937	T7936	amod	other,organs
R2204	T7938	T7926	punct	", ",detected
R2205	T7939	T7940	det	the,band
R2206	T7940	T7926	nsubjpass	band,detected
R2207	T7941	T7942	compound	RT,PCR
R2208	T7942	T7944	npadvmod	PCR,amplified
R2209	T7943	T7942	punct	-,PCR
R2210	T7944	T7940	amod	amplified,band
R2211	T7945	T7940	prep	of,band
R2212	T7946	T7947	compound	mr,s
R2213	T7947	T7945	pobj	s,of
R2214	T7948	T7947	punct	-,s
R2215	T7949	T7926	auxpass	was,detected
R2216	T7950	T7926	neg	not,detected
R2217	T7951	T7952	punct	(,2I
R2218	T7952	T7926	parataxis	2I,detected
R2219	T7953	T7952	compound	Fig.,2I
R2220	T7954	T7952	cc	and,2I
R2221	T7955	T7956	nsubj	data,shown
R2222	T7956	T7952	conj	shown,2I
R2223	T7957	T7956	neg	not,shown
R2224	T7958	T7952	punct	),2I
R2225	T7959	T7926	punct	.,detected
R2226	T7961	T7962	poss	Our,data
R2227	T7962	T7963	nsubj	data,showed
R2228	T7964	T7965	mark	that,expressed
R2229	T7965	T7963	ccomp	expressed,showed
R2230	T7966	T7967	compound	mr,s
R2231	T7967	T7965	nsubjpass	s,expressed
R2232	T7968	T7967	punct	-,s
R2233	T7969	T7965	auxpass	is,expressed
R2234	T7970	T7965	advmod	predominantly,expressed
R2235	T7971	T7965	prep	in,expressed
R2236	T7972	T7973	amod	developing,photoreceptors
R2237	T7973	T7971	pobj	photoreceptors,in
R2238	T7974	T7973	cc	and,photoreceptors
R2239	T7975	T7976	det	the,gland
R2240	T7976	T7973	conj	gland,photoreceptors
R2241	T7977	T7976	amod	pineal,gland
R2242	T7978	T7963	punct	.,showed
R2243	T9716	T9715	prep	of,Regulation
R2244	T9717	T9718	compound	mr,s
R2245	T9718	T9720	compound	s,transcription
R2246	T9719	T9718	punct	-,s
R2247	T9720	T9716	pobj	transcription,of
R2248	T9721	T9715	prep	by,Regulation
R2249	T9722	T9723	compound	Crx,protein
R2250	T9723	T9721	pobj	protein,by
R2251	T9724	T9723	compound	homeodomain,protein
R2252	T9726	T9727	compound	Transcription,factors
R2253	T9727	T9728	nsubjpass	factors,known
R2254	T9729	T9727	appos	Otx2,factors
R2255	T9730	T9729	cc	and,Otx2
R2256	T9731	T9729	conj	Crx,Otx2
R2257	T9732	T9728	auxpass	are,known
R2258	T9733	T9734	aux	to,be
R2259	T9734	T9728	xcomp	be,known
R2260	T9735	T9736	amod	key,regulators
R2261	T9736	T9734	attr	regulators,be
R2262	T9737	T9736	prep	of,regulators
R2263	T9738	T9739	amod	retinal,development
R2264	T9739	T9737	pobj	development,of
R2265	T9740	T9739	compound	photoreceptor,development
R2266	T9741	T9742	punct	[,9
R2267	T9742	T9728	parataxis	9,known
R2268	T9743	T9742	nummod	3,9
R2269	T9744	T9742	punct	",",9
R2270	T9745	T9742	nummod	8,9
R2271	T9746	T9742	punct	",",9
R2272	T9747	T9742	punct	],9
R2273	T9748	T9728	punct	.,known
R2274	T9750	T9751	mark	Although,expressed
R2275	T9751	T9757	advcl	expressed,expressed
R2276	T9752	T9753	preconj	both,Otx2
R2277	T9753	T9751	nsubjpass	Otx2,expressed
R2278	T9754	T9753	cc	and,Otx2
R2279	T9755	T9753	conj	Crx,Otx2
R2280	T9756	T9751	auxpass	are,expressed
R2281	T9758	T9751	prep	in,expressed
R2282	T9759	T9760	amod	developing,cells
R2283	T9760	T9758	pobj	cells,in
R2284	T9761	T9760	compound	photoreceptor,cells
R2285	T9762	T9751	prep	in,expressed
R2286	T9763	T9764	det	the,retina
R2287	T9764	T9762	pobj	retina,in
R2288	T9765	T9764	amod	embryonic,retina
R2289	T9766	T9757	punct	", ",expressed
R2290	T9767	T9768	advmod	only,Crx
R2291	T9768	T9757	nsubjpass	Crx,expressed
R2292	T9769	T9757	auxpass	is,expressed
R2293	T9770	T9757	advmod	highly,expressed
R2294	T9771	T9757	prep	in,expressed
R2295	T9772	T9773	det	the,photoreceptors
R2296	T9773	T9771	pobj	photoreceptors,in
R2297	T9774	T9773	amod	postnatal,photoreceptors
R2298	T9775	T9757	punct	", ",expressed
R2299	T9776	T9757	advcl	suggesting,expressed
R2300	T9777	T9778	mark	that,regulated
R2301	T9778	T9776	ccomp	regulated,suggesting
R2302	T9779	T9780	compound	mr,s
R2303	T9780	T9778	nsubjpass	s,regulated
R2304	T9781	T9780	punct	-,s
R2305	T9782	T9778	aux	may,regulated
R2306	T9783	T9778	auxpass	be,regulated
R2307	T9784	T9778	agent	by,regulated
R2308	T9785	T9784	pobj	Crx,by
R2309	T9786	T9757	punct	.,expressed
R2310	T9788	T9789	aux	To,test
R2311	T9789	T9790	advcl	test,performed
R2312	T9791	T9792	mark	whether,regulates
R2313	T9792	T9789	ccomp	regulates,test
R2314	T9793	T9792	nsubj	Crx,regulates
R2315	T9794	T9795	compound	mr,s
R2316	T9795	T9797	compound	s,transcription
R2317	T9796	T9795	punct	-,s
R2318	T9797	T9792	dobj	transcription,regulates
R2319	T9798	T9790	punct	", ",performed
R2320	T9799	T9790	nsubj	we,performed
R2321	T9800	T9801	advmod	in,situ
R2322	T9801	T9802	amod	situ,hybridization
R2323	T9802	T9790	dobj	hybridization,performed
R2324	T9803	T9802	prep	of,hybridization
R2325	T9804	T9805	compound	mr,s
R2326	T9805	T9807	compound	s,mRNA
R2327	T9806	T9805	punct	-,s
R2328	T9807	T9803	pobj	mRNA,of
R2329	T9808	T9790	prep	on,performed
R2330	T9809	T9810	amod	wild,type
R2331	T9810	T9812	nmod	type,retinas
R2332	T9811	T9810	punct	-,type
R2333	T9812	T9808	pobj	retinas,on
R2334	T9813	T9810	cc	and,type
R2335	T9814	T9815	compound	Crx,KO
R2336	T9815	T9810	conj	KO,type
R2337	T9816	T9812	compound	P5,retinas
R2338	T9817	T9818	punct	(,B
R2339	T9818	T9790	parataxis	B,performed
R2340	T9819	T9818	nmod	Fig.,B
R2341	T9820	T9818	nmod	3A,B
R2342	T9821	T9818	punct	", ",B
R2343	T9822	T9818	punct	),B
R2344	T9823	T9790	punct	.,performed
R2345	T9825	T9826	prep	In,reduced
R2346	T9827	T9828	det	the,retina
R2347	T9828	T9825	pobj	retina,In
R2348	T9829	T9830	compound	Crx,KO
R2349	T9830	T9828	compound	KO,retina
R2350	T9831	T9826	punct	", ",reduced
R2351	T9832	T9833	det	the,transcript
R2352	T9833	T9826	nsubjpass	transcript,reduced
R2353	T9834	T9835	compound	mr,s
R2354	T9835	T9833	compound	s,transcript
R2355	T9836	T9835	punct	-,s
R2356	T9837	T9826	auxpass	was,reduced
R2357	T9838	T9826	advmod	dramatically,reduced
R2358	T9839	T9840	punct	(,3B
R2359	T9840	T9826	parataxis	3B,reduced
R2360	T9841	T9840	compound	Fig.,3B
R2361	T9842	T9840	punct	),3B
R2362	T9843	T9826	punct	.,reduced
R2363	T9845	T9846	nsubj	This,indicates
R2364	T9847	T9848	mark	that,is
R2365	T9848	T9846	ccomp	is,indicates
R2366	T9849	T9848	nsubj	Crx,is
R2367	T9850	T9848	acomp	essential,is
R2368	T9851	T9850	prep	for,essential
R2369	T9852	T9851	pobj	transactivation,for
R2370	T9853	T9852	prep	of,transactivation
R2371	T9854	T9855	compound	mr,s
R2372	T9855	T9853	pobj	s,of
R2373	T9856	T9855	punct	-,s
R2374	T9857	T9846	punct	.,indicates
R2375	T9859	T9860	advmod	Moreover,used
R2376	T9861	T9860	punct	", ",used
R2377	T9862	T9863	aux	to,test
R2378	T9863	T9860	advcl	test,used
R2379	T9864	T9865	mark	whether,regulated
R2380	T9865	T9863	ccomp	regulated,test
R2381	T9866	T9867	det	the,transcription
R2382	T9867	T9865	nsubjpass	transcription,regulated
R2383	T9868	T9867	prep	of,transcription
R2384	T9869	T9870	compound	mr,s
R2385	T9870	T9868	pobj	s,of
R2386	T9871	T9870	punct	-,s
R2387	T9872	T9865	auxpass	is,regulated
R2388	T9873	T9865	advmod	also,regulated
R2389	T9874	T9865	agent	by,regulated
R2390	T9875	T9874	pobj	Crx,by
R2391	T9876	T9865	prep	in,regulated
R2392	T9877	T9878	det	the,gland
R2393	T9878	T9876	pobj	gland,in
R2394	T9879	T9878	amod	pineal,gland
R2395	T9880	T9860	punct	", ",used
R2396	T9881	T9882	compound	RT,PCR
R2397	T9882	T9884	compound	PCR,analysis
R2398	T9883	T9882	punct	-,PCR
R2399	T9884	T9860	nsubjpass	analysis,used
R2400	T9885	T9860	auxpass	was,used
R2401	T9886	T9887	aux	to,compare
R2402	T9887	T9860	advcl	compare,used
R2403	T9888	T9889	amod	transcriptional,levels
R2404	T9889	T9887	dobj	levels,compare
R2405	T9890	T9889	prep	of,levels
R2406	T9891	T9892	compound	mr,s
R2407	T9892	T9890	pobj	s,of
R2408	T9893	T9892	punct	-,s
R2409	T9894	T9887	prep	in,compare
R2410	T9895	T9896	nmod	P28,gland
R2411	T9896	T9894	pobj	gland,in
R2412	T9897	T9898	amod	wild,type
R2413	T9898	T9896	nmod	type,gland
R2414	T9899	T9898	punct	-,type
R2415	T9900	T9898	cc	and,type
R2416	T9901	T9902	compound	Crx,KO
R2417	T9902	T9898	conj	KO,type
R2418	T9903	T9896	amod	pineal,gland
R2419	T9904	T9905	punct	(,3C
R2420	T9905	T9860	parataxis	3C,used
R2421	T9906	T9905	compound	Fig.,3C
R2422	T9907	T9905	punct	),3C
R2423	T9908	T9860	punct	.,used
R2424	T9910	T9911	det	The,results
R2425	T9911	T9912	nsubj	results,revealed
R2426	T9913	T9914	mark	that,downregulated
R2427	T9914	T9912	ccomp	downregulated,revealed
R2428	T9915	T9916	compound	mr,s
R2429	T9916	T9918	compound	s,transcription
R2430	T9917	T9916	punct	-,s
R2431	T9918	T9914	nsubjpass	transcription,downregulated
R2432	T9919	T9914	auxpass	was,downregulated
R2433	T9920	T9914	advmod	significantly,downregulated
R2434	T9921	T9914	prep	in,downregulated
R2435	T9922	T9923	det	the,gland
R2436	T9923	T9921	pobj	gland,in
R2437	T9924	T9925	nmod	Crx,KO
R2438	T9925	T9923	nmod	KO,gland
R2439	T9926	T9923	amod	pineal,gland
R2440	T9927	T9912	punct	.,revealed
R2441	T9929	T9930	advcl	Taken,indicate
R2442	T9931	T9929	advmod	together,Taken
R2443	T9932	T9930	punct	", ",indicate
R2444	T9933	T9934	det	these,data
R2445	T9934	T9930	nsubj	data,indicate
R2446	T9935	T9936	mark	that,regulated
R2447	T9936	T9930	ccomp	regulated,indicate
R2448	T9937	T9938	compound	mr,s
R2449	T9938	T9940	compound	s,transcription
R2450	T9939	T9938	punct	-,s
R2451	T9940	T9936	nsubjpass	transcription,regulated
R2452	T9941	T9936	auxpass	is,regulated
R2453	T9942	T9936	agent	by,regulated
R2454	T9943	T9942	pobj	Crx,by
R2455	T9944	T9936	prep	in,regulated
R2456	T9945	T9946	det	the,photoreceptors
R2457	T9946	T9944	pobj	photoreceptors,in
R2458	T9947	T9946	amod	developing,photoreceptors
R2459	T9948	T9946	cc	and,photoreceptors
R2460	T9949	T9950	amod	pineal,gland
R2461	T9950	T9946	conj	gland,photoreceptors
R2462	T9951	T9930	punct	.,indicate
R2463	T9954	T9955	det	T,o
R2464	T9955	T9956	nsubjpass	o,u
R2465	T9957	T9955	prep	f,o
R2466	T9960	T9961	amod	t,h
R2467	T9963	T9962	prep	r,e
R2468	T9969	T9970	nsubjpass	in,e
R2469	T9980	T9979	compound	C,r
R2470	T9983	T9982	nsubj	x,re
R2471	T9989	T9988	cc	a,l
R2472	T9995	T9993	auxpass	r,-s
R2473	T9996	T9993	prep	t,-s
R2474	T9999	T10000	nsubj	a,n
R2475	T10008	T10003	punct	", ",performed
R2476	T10009	T10003	nsubj	we,performed
R2477	T10010	T10003	advmod	next,performed
R2478	T10011	T10012	det	a,assay
R2479	T10012	T10003	dobj	assay,performed
R2480	T10013	T10012	compound	luciferase,assay
R2481	T10014	T10003	advcl	using,performed
R2482	T10015	T10016	det	the,region
R2483	T10016	T10014	dobj	region,using
R2484	T10017	T10018	nummod	1.2,kb
R2485	T10018	T10016	nmod	kb,region
R2486	T10019	T10018	punct	-,kb
R2487	T10020	T10016	amod	proximal,region
R2488	T10021	T10016	compound	promoter,region
R2489	T10022	T10016	prep	of,region
R2490	T10023	T10024	compound	mr,s
R2491	T10024	T10022	pobj	s,of
R2492	T10025	T10024	punct	-,s
R2493	T10026	T10016	acl	fused,region
R2494	T10027	T10026	prep	to,fused
R2495	T10028	T10029	det	a,gene
R2496	T10029	T10027	pobj	gene,to
R2497	T10030	T10029	compound	luciferase,gene
R2498	T10031	T10029	prep	as,gene
R2499	T10032	T10033	det	a,reporter
R2500	T10033	T10031	pobj	reporter,as
R2501	T10034	T10033	compound	luciferase,reporter
R2502	T10035	T10036	punct	(,3D
R2503	T10036	T10033	parataxis	3D,reporter
R2504	T10037	T10036	compound	Fig.,3D
R2505	T10038	T10036	punct	", ",3D
R2506	T10039	T10036	appos	Pro1.2k,3D
R2507	T10040	T10036	punct	),3D
R2508	T10041	T10029	cc	and,gene
R2509	T10042	T10043	det	the,vectors
R2510	T10043	T10029	conj	vectors,gene
R2511	T10044	T10045	nmod	Crx,Nrl
R2512	T10045	T10043	compound	Nrl,vectors
R2513	T10046	T10045	punct	", ",Nrl
R2514	T10047	T10045	nmod	Otx2,Nrl
R2515	T10048	T10045	punct	", ",Nrl
R2516	T10049	T10043	compound	expression,vectors
R2517	T10050	T10026	punct	", ",fused
R2518	T10051	T10026	advmod	respectively,fused
R2519	T10052	T10003	punct	.,performed
R2520	T10054	T10055	det	This,region
R2521	T10055	T10059	nsubj	region,contains
R2522	T10056	T10057	nummod	1.2,kb
R2523	T10057	T10055	compound	kb,region
R2524	T10058	T10057	punct	-,kb
R2525	T10060	T10055	prep	of,region
R2526	T10061	T10062	det	the,sequence
R2527	T10062	T10060	pobj	sequence,of
R2528	T10063	T10064	nmod	mr,s
R2529	T10064	T10062	nmod	s,sequence
R2530	T10065	T10064	punct	-,s
R2531	T10066	T10062	amod	upstream,sequence
R2532	T10067	T10068	nummod	three,sequences
R2533	T10068	T10059	dobj	sequences,contains
R2534	T10069	T10070	compound	Crx,binding
R2535	T10070	T10068	compound	binding,sequences
R2536	T10071	T10068	compound	consensus,sequences
R2537	T10072	T10059	punct	.,contains
R2538	T10074	T10075	mark	As,shown
R2539	T10075	T10076	advcl	shown,upregulated
R2540	T10077	T10075	prep	in,shown
R2541	T10078	T10079	compound	Fig.,3E
R2542	T10079	T10077	pobj	3E,in
R2543	T10080	T10076	punct	", ",upregulated
R2544	T10081	T10082	det	the,activity
R2545	T10082	T10076	nsubjpass	activity,upregulated
R2546	T10083	T10082	compound	luciferase,activity
R2547	T10084	T10076	auxpass	was,upregulated
R2548	T10085	T10076	advmod	significantly,upregulated
R2549	T10086	T10087	advmod	when,co-introduced
R2550	T10087	T10076	advcl	co-introduced,upregulated
R2551	T10088	T10089	det	the,vector
R2552	T10089	T10087	nsubjpass	vector,co-introduced
R2553	T10090	T10089	nmod	Crx,vector
R2554	T10091	T10090	cc	or,Crx
R2555	T10092	T10090	conj	Otx2,Crx
R2556	T10093	T10089	compound	expression,vector
R2557	T10094	T10087	auxpass	was,co-introduced
R2558	T10095	T10087	prep	with,co-introduced
R2559	T10096	T10095	pobj	Pro1.2k,with
R2560	T10097	T10087	prep	into,co-introduced
R2561	T10098	T10099	compound	HEK293T,cells
R2562	T10099	T10097	pobj	cells,into
R2563	T10100	T10087	punct	", ",co-introduced
R2564	T10101	T10102	mark	while,altered
R2565	T10102	T10087	advcl	altered,co-introduced
R2566	T10103	T10104	det	the,activity
R2567	T10104	T10102	nsubjpass	activity,altered
R2568	T10105	T10104	compound	luciferase,activity
R2569	T10106	T10102	auxpass	was,altered
R2570	T10107	T10102	neg	not,altered
R2571	T10108	T10109	advmod	when,co-introduced
R2572	T10109	T10102	advcl	co-introduced,altered
R2573	T10110	T10111	det	the,vector
R2574	T10111	T10109	nsubjpass	vector,co-introduced
R2575	T10112	T10111	compound	Nrl,vector
R2576	T10113	T10111	compound	expression,vector
R2577	T10114	T10109	auxpass	was,co-introduced
R2578	T10115	T10076	punct	.,upregulated
R2579	T10117	T10118	det	A,report
R2580	T10118	T10120	nsubj	report,suggested
R2581	T10119	T10118	amod	previous,report
R2582	T10121	T10122	mark	that,augmented
R2583	T10122	T10120	ccomp	augmented,suggested
R2584	T10123	T10124	det	the,activity
R2585	T10124	T10122	nsubjpass	activity,augmented
R2586	T10125	T10124	amod	transcriptional,activity
R2587	T10126	T10124	prep	of,activity
R2588	T10127	T10126	pobj	Crx,of
R2589	T10128	T10122	auxpass	is,augmented
R2590	T10129	T10122	prep	with,augmented
R2591	T10130	T10129	pobj	Nrl,with
R2592	T10131	T10132	advmod	when,used
R2593	T10132	T10122	advcl	used,augmented
R2594	T10133	T10134	det	the,promoter
R2595	T10134	T10132	nsubjpass	promoter,used
R2596	T10135	T10134	compound	rhodopsin,promoter
R2597	T10136	T10132	auxpass	was,used
R2598	T10137	T10132	prep	as,used
R2599	T10138	T10139	det	a,gene
R2600	T10139	T10137	pobj	gene,as
R2601	T10140	T10139	compound	reporter,gene
R2602	T10141	T10142	punct	[,6
R2603	T10142	T10120	parataxis	6,suggested
R2604	T10143	T10142	punct	],6
R2605	T10144	T10120	punct	.,suggested
R2606	T10146	T10147	prep	On,showed
R2607	T10148	T10149	det	the,hand
R2608	T10149	T10146	pobj	hand,On
R2609	T10150	T10149	amod	other,hand
R2610	T10151	T10147	punct	", ",showed
R2611	T10152	T10153	poss	our,data
R2612	T10153	T10147	nsubj	data,showed
R2613	T10154	T10153	amod	present,data
R2614	T10155	T10156	mark	that,upregulated
R2615	T10156	T10147	ccomp	upregulated,showed
R2616	T10157	T10158	det	the,expression
R2617	T10158	T10156	nsubjpass	expression,upregulated
R2618	T10159	T10158	compound	luciferase,expression
R2619	T10160	T10158	compound	gene,expression
R2620	T10161	T10156	auxpass	was,upregulated
R2621	T10162	T10156	neg	not,upregulated
R2622	T10163	T10164	advmod	when,co-introduced
R2623	T10164	T10156	advcl	co-introduced,upregulated
R2624	T10165	T10166	preconj	both,Crx
R2625	T10166	T10167	nmod	Crx,vectors
R2626	T10167	T10164	nsubjpass	vectors,co-introduced
R2627	T10168	T10166	cc	and,Crx
R2628	T10169	T10166	conj	Nrl,Crx
R2629	T10170	T10167	compound	expression,vectors
R2630	T10171	T10164	auxpass	were,co-introduced
R2631	T10172	T10164	prep	with,co-introduced
R2632	T10173	T10172	pobj	Pro1.2k,with
R2633	T10174	T10156	prep	compared,upregulated
R2634	T10175	T10174	prep	to,compared
R2635	T10176	T10177	det	the,activity
R2636	T10177	T10175	pobj	activity,to
R2637	T10178	T10179	advmod	when,introduced
R2638	T10179	T10177	relcl	introduced,activity
R2639	T10180	T10181	det	the,vector
R2640	T10181	T10179	nsubjpass	vector,introduced
R2641	T10182	T10181	nmod	Crx,vector
R2642	T10183	T10182	amod	only,Crx
R2643	T10184	T10181	compound	expression,vector
R2644	T10185	T10179	auxpass	was,introduced
R2645	T10186	T10147	punct	.,showed
R2646	T10188	T10189	nsubj	This,be
R2647	T10190	T10189	aux	may,be
R2648	T10191	T10189	prep	due,be
R2649	T10192	T10191	pcomp	to,due
R2650	T10193	T10194	compound	cell,type
R2651	T10194	T10195	compound	type,differences
R2652	T10195	T10191	pobj	differences,due
R2653	T10196	T10197	mark	because,used
R2654	T10197	T10189	advcl	used,be
R2655	T10198	T10199	det	a,type
R2656	T10199	T10197	nsubjpass	type,used
R2657	T10200	T10199	compound	cell,type
R2658	T10201	T10199	prep	of,type
R2659	T10202	T10203	amod	retinal,pineal
R2660	T10203	T10205	amod	pineal,origin
R2661	T10204	T10203	punct	/,pineal
R2662	T10205	T10201	pobj	origin,of
R2663	T10206	T10197	auxpass	was,used
R2664	T10207	T10197	neg	not,used
R2665	T10208	T10197	prep	in,used
R2666	T10209	T10210	poss	our,assay
R2667	T10210	T10208	pobj	assay,in
R2668	T10211	T10210	compound	luciferase,assay
R2669	T10212	T10189	punct	.,be
R2670	T10214	T10215	prep	In,showed
R2671	T10216	T10214	pobj	addition,In
R2672	T10217	T10215	punct	", ",showed
R2673	T10218	T10219	poss	our,data
R2674	T10219	T10215	nsubj	data,showed
R2675	T10220	T10219	amod	present,data
R2676	T10221	T10222	mark	that,transactivated
R2677	T10222	T10215	ccomp	transactivated,showed
R2678	T10223	T10222	nsubj	Otx2,transactivated
R2679	T10224	T10223	punct	", ",Otx2
R2680	T10225	T10226	dep	which,reported
R2681	T10226	T10223	relcl	reported,Otx2
R2682	T10227	T10226	auxpass	is,reported
R2683	T10228	T10229	aux	to,have
R2684	T10229	T10226	xcomp	have,reported
R2685	T10230	T10231	det	the,consensus
R2686	T10231	T10229	dobj	consensus,have
R2687	T10232	T10231	amod	same,consensus
R2688	T10233	T10231	compound	binding,consensus
R2689	T10234	T10231	prep	as,consensus
R2690	T10235	T10234	pobj	Crx,as
R2691	T10236	T10222	punct	", ",transactivated
R2692	T10237	T10222	advmod	also,transactivated
R2693	T10238	T10239	compound	mr,s
R2694	T10239	T10241	compound	s,expression
R2695	T10240	T10239	punct	-,s
R2696	T10241	T10222	dobj	expression,transactivated
R2697	T10242	T10215	punct	.,showed
R2698	T10244	T10245	mark	As,shown
R2699	T10245	T10246	advcl	shown,correlates
R2700	T10247	T10245	prep	in,shown
R2701	T10248	T10249	compound	Fig.,2A
R2702	T10249	T10247	pobj	2A,in
R2703	T10250	T10251	punct	–,F
R2704	T10251	T10249	prep	F,2A
R2705	T10252	T10246	punct	", ",correlates
R2706	T10253	T10254	det	the,pattern
R2707	T10254	T10246	nsubj	pattern,correlates
R2708	T10255	T10254	compound	expression,pattern
R2709	T10256	T10254	prep	of,pattern
R2710	T10257	T10258	compound	mr,s
R2711	T10258	T10256	pobj	s,of
R2712	T10259	T10258	punct	-,s
R2713	T10260	T10246	prep	with,correlates
R2714	T10261	T10260	pobj	that,with
R2715	T10262	T10261	prep	of,that
R2716	T10263	T10262	pobj	Crx,of
R2717	T10264	T10246	punct	.,correlates
R2718	T10266	T10267	prep	In,detected
R2719	T10268	T10266	pobj	contrast,In
R2720	T10269	T10267	punct	", ",detected
R2721	T10270	T10271	det	the,transcripts
R2722	T10271	T10267	nsubjpass	transcripts,detected
R2723	T10272	T10271	prep	of,transcripts
R2724	T10273	T10272	pobj	Otx2,of
R2725	T10274	T10267	auxpass	are,detected
R2726	T10275	T10267	advmod	mainly,detected
R2727	T10276	T10267	prep	in,detected
R2728	T10277	T10278	det	the,layer
R2729	T10278	T10276	pobj	layer,in
R2730	T10279	T10278	compound	photoreceptor,layer
R2731	T10280	T10267	prep	at,detected
R2732	T10281	T10282	amod	embryonic,stages
R2733	T10282	T10280	pobj	stages,at
R2734	T10283	T10267	punct	.,detected
R2735	T10285	T10286	advmod	Therefore,concluded
R2736	T10287	T10286	punct	", ",concluded
R2737	T10288	T10286	nsubj	we,concluded
R2738	T10289	T10290	mark	that,regulated
R2739	T10290	T10286	ccomp	regulated,concluded
R2740	T10291	T10292	compound	mr,s
R2741	T10292	T10294	compound	s,transcription
R2742	T10293	T10292	punct	-,s
R2743	T10294	T10290	nsubjpass	transcription,regulated
R2744	T10295	T10290	auxpass	is,regulated
R2745	T10296	T10290	advmod	directly,regulated
R2746	T10297	T10298	advmod	mainly,by
R2747	T10298	T10290	agent	by,regulated
R2748	T10299	T10298	pobj	Crx,by
R2749	T10300	T10286	punct	.,concluded
R2750	T10302	T10303	nsubj	We,constructed
R2751	T10304	T10303	advmod	also,constructed
R2752	T10305	T10306	compound	reporter,vectors
R2753	T10306	T10303	dobj	vectors,constructed
R2754	T10307	T10308	prep	in,introduced
R2755	T10308	T10306	relcl	introduced,vectors
R2756	T10309	T10307	pobj	which,in
R2757	T10310	T10308	nsubjpass	mutations,introduced
R2758	T10311	T10308	auxpass	were,introduced
R2759	T10312	T10308	prep	at,introduced
R2760	T10313	T10314	det	the,sites
R2761	T10314	T10312	pobj	sites,at
R2762	T10315	T10314	nummod	three,sites
R2763	T10316	T10317	compound	Crx,binding
R2764	T10317	T10314	compound	binding,sites
R2765	T10318	T10319	punct	(,mut1259
R2766	T10319	T10303	parataxis	mut1259,constructed
R2767	T10320	T10321	compound	Fig.,3D
R2768	T10321	T10319	dep	3D,mut1259
R2769	T10322	T10319	punct	", ",mut1259
R2770	T10323	T10319	punct	", ",mut1259
R2771	T10324	T10319	appos	mut198,mut1259
R2772	T10325	T10319	punct	", ",mut1259
R2773	T10326	T10319	appos	mut72,mut1259
R2774	T10327	T10319	punct	", ",mut1259
R2775	T10328	T10329	compound	mut,all
R2776	T10329	T10319	appos	all,mut1259
R2777	T10330	T10319	punct	),mut1259
R2778	T10331	T10303	punct	.,constructed
R2779	T10333	T10334	advmod	Then,co-introduced
R2780	T10335	T10336	det	the,vector
R2781	T10336	T10334	nsubjpass	vector,co-introduced
R2782	T10337	T10336	compound	Crx,vector
R2783	T10338	T10336	compound	expression,vector
R2784	T10339	T10334	auxpass	was,co-introduced
R2785	T10340	T10334	prep	with,co-introduced
R2786	T10341	T10340	pobj	mut1259,with
R2787	T10342	T10341	punct	", ",mut1259
R2788	T10343	T10341	conj	mut198,mut1259
R2789	T10344	T10343	punct	", ",mut198
R2790	T10345	T10343	conj	mut72,mut198
R2791	T10346	T10345	cc	and,mut72
R2792	T10347	T10348	compound	mut,all
R2793	T10348	T10345	conj	all,mut72
R2794	T10349	T10334	punct	", ",co-introduced
R2795	T10350	T10334	advmod	respectively,co-introduced
R2796	T10351	T10352	punct	(,3F
R2797	T10352	T10334	parataxis	3F,co-introduced
R2798	T10353	T10352	compound	Fig.,3F
R2799	T10354	T10352	punct	),3F
R2800	T10355	T10334	punct	.,co-introduced
R2801	T10357	T10358	det	The,activity
R2802	T10358	T10360	nsubjpass	activity,reduced
R2803	T10359	T10358	compound	transactivaton,activity
R2804	T10361	T10358	prep	by,activity
R2805	T10362	T10361	pobj	Crx,by
R2806	T10363	T10360	auxpass	was,reduced
R2807	T10364	T10360	advmod	clearly,reduced
R2808	T10365	T10366	advmod	when,co-introduced
R2809	T10366	T10360	advcl	co-introduced,reduced
R2810	T10367	T10366	nsubjpass	mut198,co-introduced
R2811	T10368	T10367	cc	or,mut198
R2812	T10369	T10367	conj	mut72,mut198
R2813	T10370	T10366	auxpass	was,co-introduced
R2814	T10371	T10360	punct	.,reduced
R2815	T10373	T10374	prep	On,altered
R2816	T10375	T10376	det	the,hand
R2817	T10376	T10373	pobj	hand,On
R2818	T10377	T10376	amod	other,hand
R2819	T10378	T10374	punct	", ",altered
R2820	T10379	T10380	advmod	when,co-transfected
R2821	T10380	T10374	advcl	co-transfected,altered
R2822	T10381	T10380	nsubjpass	mut1259,co-transfected
R2823	T10382	T10380	auxpass	was,co-transfected
R2824	T10383	T10374	punct	", ",altered
R2825	T10384	T10385	det	the,activity
R2826	T10385	T10374	nsubjpass	activity,altered
R2827	T10386	T10385	compound	transactivation,activity
R2828	T10387	T10385	prep	by,activity
R2829	T10388	T10387	pobj	Crx,by
R2830	T10389	T10374	auxpass	was,altered
R2831	T10390	T10374	neg	not,altered
R2832	T10391	T10374	punct	.,altered
R2833	T10393	T10394	det	These,results
R2834	T10394	T10395	nsubj	results,suggest
R2835	T10396	T10397	mark	that,are
R2836	T10397	T10395	ccomp	are,suggest
R2837	T10398	T10399	det	the,sites
R2838	T10399	T10397	nsubj	sites,are
R2839	T10400	T10401	compound	Crx,binding
R2840	T10401	T10399	compound	binding,sites
R2841	T10402	T10403	nummod	72,bp
R2842	T10403	T10405	npadvmod	bp,upstream
R2843	T10404	T10403	punct	-,bp
R2844	T10405	T10399	advmod	upstream,sites
R2845	T10406	T10403	cc	and,bp
R2846	T10407	T10408	nummod	198,bp
R2847	T10408	T10403	conj	bp,bp
R2848	T10409	T10408	punct	-,bp
R2849	T10410	T10405	prep	from,upstream
R2850	T10411	T10412	det	the,site
R2851	T10412	T10410	pobj	site,from
R2852	T10413	T10412	compound	transcription,site
R2853	T10414	T10412	compound	initiation,site
R2854	T10415	T10397	acomp	crucial,are
R2855	T10416	T10415	prep	for,crucial
R2856	T10417	T10418	det	the,regulation
R2857	T10418	T10416	pobj	regulation,for
R2858	T10419	T10418	amod	direct,regulation
R2859	T10420	T10418	prep	of,regulation
R2860	T10421	T10422	compound	mr,s
R2861	T10422	T10424	compound	s,transcription
R2862	T10423	T10422	punct	-,s
R2863	T10424	T10420	pobj	transcription,of
R2864	T10425	T10418	prep	by,regulation
R2865	T10426	T10425	pobj	Crx,by
R2866	T10427	T10395	punct	.,suggest
R2867	T12462	T12463	compound	Self,association
R2868	T12464	T12463	punct	-,association
R2869	T12465	T12463	prep	of,association
R2870	T12466	T12467	compound	mr,s
R2871	T12467	T12469	compound	s,protein
R2872	T12468	T12467	punct	-,s
R2873	T12469	T12465	pobj	protein,of
R2874	T12471	T12472	compound	SAM,domains
R2875	T12472	T12473	nsubjpass	domains,known
R2876	T12474	T12473	auxpass	are,known
R2877	T12475	T12476	aux	to,function
R2878	T12476	T12473	xcomp	function,known
R2879	T12477	T12476	prep	as,function
R2880	T12478	T12479	compound	protein,protein
R2881	T12479	T12481	compound	protein,modules
R2882	T12480	T12479	punct	-,protein
R2883	T12481	T12477	pobj	modules,as
R2884	T12482	T12481	compound	interaction,modules
R2885	T12483	T12484	punct	[,15
R2886	T12484	T12473	parataxis	15,known
R2887	T12485	T12486	punct	-,17
R2888	T12486	T12484	prep	17,15
R2889	T12487	T12484	punct	],15
R2890	T12488	T12473	punct	.,known
R2891	T12490	T12491	mark	Although,bind
R2892	T12491	T12495	advcl	bind,reported
R2893	T12492	T12493	compound	SAM,domains
R2894	T12493	T12491	nsubj	domains,bind
R2895	T12494	T12491	aux	can,bind
R2896	T12496	T12491	prep	to,bind
R2897	T12497	T12498	amod	various,proteins
R2898	T12498	T12496	pobj	proteins,to
R2899	T12499	T12500	amod	non-SAM,domain
R2900	T12500	T12501	npadvmod	domain,containing
R2901	T12501	T12498	amod	containing,proteins
R2902	T12502	T12501	punct	-,containing
R2903	T12503	T12495	punct	", ",reported
R2904	T12504	T12505	amod	many,interactions
R2905	T12505	T12495	nsubjpass	interactions,reported
R2906	T12506	T12505	nmod	homo-SAM,interactions
R2907	T12507	T12506	cc	and,homo-SAM
R2908	T12508	T12506	conj	hetero-SAM,homo-SAM
R2909	T12509	T12505	compound	domain,interactions
R2910	T12510	T12495	aux	have,reported
R2911	T12511	T12495	auxpass	been,reported
R2912	T12512	T12495	punct	.,reported
R2913	T12514	T12515	aux	To,investigate
R2914	T12515	T12516	advcl	investigate,performed
R2915	T12517	T12518	mark	whether,function
R2916	T12518	T12515	ccomp	function,investigate
R2917	T12519	T12520	det	the,domain
R2918	T12520	T12518	nsubj	domain,function
R2919	T12521	T12520	compound	SAM,domain
R2920	T12522	T12520	prep	of,domain
R2921	T12523	T12524	det	the,protein
R2922	T12524	T12522	pobj	protein,of
R2923	T12525	T12526	compound	mr,s
R2924	T12526	T12524	compound	s,protein
R2925	T12527	T12526	punct	-,s
R2926	T12528	T12518	aux	can,function
R2927	T12529	T12518	advmod	also,function
R2928	T12530	T12518	prep	as,function
R2929	T12531	T12532	det	a,module
R2930	T12532	T12530	pobj	module,as
R2931	T12533	T12534	compound	protein,protein
R2932	T12534	T12532	compound	protein,module
R2933	T12535	T12534	punct	-,protein
R2934	T12536	T12532	compound	interaction,module
R2935	T12537	T12516	punct	", ",performed
R2936	T12538	T12516	nsubj	we,performed
R2937	T12539	T12540	nmod	yeast,screening
R2938	T12540	T12516	dobj	screening,performed
R2939	T12541	T12542	nummod	two,hybrid
R2940	T12542	T12540	compound	hybrid,screening
R2941	T12543	T12542	punct	-,hybrid
R2942	T12544	T12516	advcl	using,performed
R2943	T12545	T12546	amod	full,length
R2944	T12546	T12548	compound	length,protein
R2945	T12547	T12546	punct	-,length
R2946	T12548	T12544	dobj	protein,using
R2947	T12549	T12550	compound	mr,s
R2948	T12550	T12548	compound	s,protein
R2949	T12551	T12550	punct	-,s
R2950	T12552	T12544	prep	as,using
R2951	T12553	T12554	det	the,bait
R2952	T12554	T12552	pobj	bait,as
R2953	T12555	T12516	punct	.,performed
R2954	T12557	T12558	advcl	Using,screened
R2955	T12559	T12560	det	this,bait
R2956	T12560	T12557	dobj	bait,Using
R2957	T12561	T12558	punct	", ",screened
R2958	T12562	T12558	nsubj	we,screened
R2959	T12563	T12564	det	the,library
R2960	T12564	T12558	dobj	library,screened
R2961	T12565	T12566	amod	transcriptional,activator
R2962	T12566	T12567	compound	activator,protein
R2963	T12567	T12564	compound	protein,library
R2964	T12568	T12567	compound	fusion,protein
R2965	T12569	T12570	prep	in,fused
R2966	T12570	T12564	advcl	fused,library
R2967	T12571	T12569	pobj	which,in
R2968	T12572	T12573	nmod	mouse,cDNAs
R2969	T12573	T12570	nsubjpass	cDNAs,fused
R2970	T12574	T12573	nmod	P0,cDNAs
R2971	T12575	T12576	punct	-,P3
R2972	T12576	T12574	prep	P3,P0
R2973	T12577	T12573	amod	retinal,cDNAs
R2974	T12578	T12570	auxpass	were,fused
R2975	T12579	T12570	prep	to,fused
R2976	T12580	T12581	det	the,domain
R2977	T12581	T12579	pobj	domain,to
R2978	T12582	T12581	compound	GAL4,domain
R2979	T12583	T12581	compound	activation,domain
R2980	T12584	T12558	punct	.,screened
R2981	T12586	T12587	det	The,clones
R2982	T12587	T12591	nsubj	clones,were
R2983	T12588	T12589	advmod	most,frequent
R2984	T12589	T12587	amod	frequent,clones
R2985	T12590	T12587	amod	positive,clones
R2986	T12592	T12593	punct	(,28
R2987	T12593	T12587	parataxis	28,clones
R2988	T12594	T12593	quantmod	5,28
R2989	T12595	T12593	quantmod	out,28
R2990	T12596	T12593	quantmod	of,28
R2991	T12597	T12593	punct	),28
R2992	T12598	T12599	compound	cDNA,fragments
R2993	T12599	T12591	attr	fragments,were
R2994	T12600	T12599	acl	containing,fragments
R2995	T12601	T12602	det	the,domain
R2996	T12602	T12600	dobj	domain,containing
R2997	T12603	T12602	compound	SAM,domain
R2998	T12604	T12602	prep	of,domain
R2999	T12605	T12606	compound	mr,s
R3000	T12606	T12604	pobj	s,of
R3001	T12607	T12606	punct	-,s
R3002	T12608	T12609	punct	(,4A
R3003	T12609	T12591	parataxis	4A,were
R3004	T12610	T12609	compound	Fig.,4A
R3005	T12611	T12609	punct	),4A
R3006	T12612	T12591	punct	.,were
R3007	T12614	T12615	det	This,result
R3008	T12615	T12616	nsubj	result,suggests
R3009	T12617	T12616	advmod	strongly,suggests
R3010	T12618	T12619	mark	that,associates
R3011	T12619	T12616	ccomp	associates,suggests
R3012	T12620	T12621	compound	mr,s
R3013	T12621	T12623	compound	s,protein
R3014	T12622	T12621	punct	-,s
R3015	T12623	T12619	nsubj	protein,associates
R3016	T12624	T12619	dep	self,associates
R3017	T12625	T12619	punct	-,associates
R3018	T12626	T12619	prep	through,associates
R3019	T12627	T12628	compound	SAM,domain
R3020	T12628	T12629	npadvmod	domain,containing
R3021	T12629	T12631	amod	containing,regions
R3022	T12630	T12629	punct	-,containing
R3023	T12631	T12626	pobj	regions,through
R3024	T12632	T12616	punct	.,suggests
R3025	T12634	T12635	nsubj	We,tested
R3026	T12636	T12635	advmod	then,tested
R3027	T12637	T12635	advmod	directly,tested
R3028	T12638	T12639	det	this,association
R3029	T12639	T12635	dobj	association,tested
R3030	T12640	T12639	compound	self,association
R3031	T12641	T12639	punct	-,association
R3032	T12642	T12639	prep	of,association
R3033	T12643	T12644	compound	mr,s
R3034	T12644	T12646	compound	s,protein
R3035	T12645	T12644	punct	-,s
R3036	T12646	T12642	pobj	protein,of
R3037	T12647	T12635	prep	in,tested
R3038	T12648	T12647	pobj	yeast,in
R3039	T12649	T12635	punct	.,tested
R3040	T12651	T12652	nsubj	We,fused
R3041	T12653	T12654	amod	full,length
R3042	T12654	T12656	nmod	length,portions
R3043	T12655	T12654	punct	-,length
R3044	T12656	T12652	dobj	portions,fused
R3045	T12657	T12654	cc	or,length
R3046	T12658	T12654	conj	truncated,length
R3047	T12659	T12656	prep	of,portions
R3048	T12660	T12661	det	the,protein
R3049	T12661	T12659	pobj	protein,of
R3050	T12662	T12663	compound	mr,s
R3051	T12663	T12661	compound	s,protein
R3052	T12664	T12663	punct	-,s
R3053	T12665	T12652	prep	to,fused
R3054	T12666	T12667	det	the,domain
R3055	T12667	T12665	pobj	domain,to
R3056	T12668	T12669	npadvmod	DNA,binding
R3057	T12669	T12667	amod	binding,domain
R3058	T12670	T12669	punct	-,binding
R3059	T12671	T12667	prep	of,domain
R3060	T12672	T12673	det	the,factor
R3061	T12673	T12671	pobj	factor,of
R3062	T12674	T12673	compound	yeast,factor
R3063	T12675	T12673	compound	transcription,factor
R3064	T12676	T12673	appos	GAL4,factor
R3065	T12677	T12678	aux	to,make
R3066	T12678	T12652	advcl	make,fused
R3067	T12679	T12680	compound	bait,constructs
R3068	T12680	T12678	dobj	constructs,make
R3069	T12681	T12652	punct	.,fused
R3070	T12683	T12684	nsubj	We,fused
R3071	T12685	T12686	amod	full,length
R3072	T12686	T12688	nmod	length,portions
R3073	T12687	T12686	punct	-,length
R3074	T12688	T12684	dobj	portions,fused
R3075	T12689	T12686	cc	or,length
R3076	T12690	T12686	conj	truncated,length
R3077	T12691	T12688	prep	of,portions
R3078	T12692	T12693	det	the,protein
R3079	T12693	T12691	pobj	protein,of
R3080	T12694	T12695	compound	mr,s
R3081	T12695	T12693	compound	s,protein
R3082	T12696	T12695	punct	-,s
R3083	T12697	T12684	prep	to,fused
R3084	T12698	T12699	det	the,domain
R3085	T12699	T12697	pobj	domain,to
R3086	T12700	T12701	nmod	GAL4,activation
R3087	T12701	T12699	compound	activation,domain
R3088	T12702	T12701	amod	transcriptional,activation
R3089	T12703	T12704	aux	to,make
R3090	T12704	T12684	advcl	make,fused
R3091	T12705	T12706	compound	prey,constructs
R3092	T12706	T12704	dobj	constructs,make
R3093	T12707	T12708	punct	(,4B
R3094	T12708	T12684	parataxis	4B,fused
R3095	T12709	T12708	compound	Fig.,4B
R3096	T12710	T12708	punct	),4B
R3097	T12711	T12684	punct	.,fused
R3098	T12713	T12714	det	These,constructs
R3099	T12714	T12715	nsubjpass	constructs,transformed
R3100	T12716	T12715	auxpass	were,transformed
R3101	T12717	T12715	prep	into,transformed
R3102	T12718	T12717	pobj	yeast,into
R3103	T12719	T12720	dep	that,contain
R3104	T12720	T12718	relcl	contain,yeast
R3105	T12721	T12722	det	a,transgene
R3106	T12722	T12720	dobj	transgene,contain
R3107	T12723	T12722	prep	with,transgene
R3108	T12724	T12725	compound	GAL4,binding
R3109	T12725	T12726	compound	binding,sites
R3110	T12726	T12723	pobj	sites,with
R3111	T12727	T12726	advmod	upstream,sites
R3112	T12728	T12727	prep	of,upstream
R3113	T12729	T12730	det	the,gene
R3114	T12730	T12728	pobj	gene,of
R3115	T12731	T12730	compound	lacZ,gene
R3116	T12732	T12715	punct	.,transformed
R3117	T12734	T12735	nsubj	We,found
R3118	T12736	T12737	mark	that,induced
R3119	T12737	T12735	ccomp	induced,found
R3120	T12738	T12739	det	the,construct
R3121	T12739	T12737	nsubj	construct,induced
R3122	T12740	T12741	amod	full,length
R3123	T12741	T12739	compound	length,construct
R3124	T12742	T12741	punct	-,length
R3125	T12743	T12744	compound	mr,s
R3126	T12744	T12739	compound	s,construct
R3127	T12745	T12744	punct	-,s
R3128	T12746	T12739	compound	bait,construct
R3129	T12747	T12748	compound	lacZ,expression
R3130	T12748	T12737	dobj	expression,induced
R3131	T12749	T12737	prep	with,induced
R3132	T12750	T12751	det	the,construct
R3133	T12751	T12749	pobj	construct,with
R3134	T12752	T12753	amod	full,length
R3135	T12753	T12751	compound	length,construct
R3136	T12754	T12753	punct	-,length
R3137	T12755	T12756	compound	mr,s
R3138	T12756	T12751	compound	s,construct
R3139	T12757	T12756	punct	-,s
R3140	T12758	T12751	compound	prey,construct
R3141	T12759	T12760	punct	(,full
R3142	T12760	T12735	parataxis	full,found
R3143	T12761	T12762	compound	Fig.,4B
R3144	T12762	T12760	dep	4B,full
R3145	T12763	T12760	punct	", ",full
R3146	T12764	T12760	amod	full,full
R3147	T12765	T12760	punct	×,full
R3148	T12766	T12760	punct	),full
R3149	T12767	T12735	punct	.,found
R3150	T12769	T12770	det	The,stretch
R3151	T12770	T12780	nsubj	stretch,activate
R3152	T12771	T12772	nmod	N,terminus
R3153	T12772	T12770	nmod	terminus,stretch
R3154	T12773	T12772	punct	-,terminus
R3155	T12774	T12770	nummod	400,stretch
R3156	T12775	T12776	nmod	amino,acid
R3157	T12776	T12770	nmod	acid,stretch
R3158	T12777	T12776	punct	(,acid
R3159	T12778	T12776	appos	aa,acid
R3160	T12779	T12770	punct	),stretch
R3161	T12781	T12770	prep	of,stretch
R3162	T12782	T12783	compound	mr,s
R3163	T12783	T12781	pobj	s,of
R3164	T12784	T12783	punct	-,s
R3165	T12785	T12770	punct	", ",stretch
R3166	T12786	T12787	dep	which,contain
R3167	T12787	T12770	relcl	contain,stretch
R3168	T12788	T12787	aux	does,contain
R3169	T12789	T12787	neg	not,contain
R3170	T12790	T12791	det	a,domain
R3171	T12791	T12787	dobj	domain,contain
R3172	T12792	T12791	compound	SAM,domain
R3173	T12793	T12780	punct	", ",activate
R3174	T12794	T12780	aux	does,activate
R3175	T12795	T12780	advmod	weakly,activate
R3176	T12796	T12780	dobj	transcription,activate
R3177	T12797	T12796	prep	of,transcription
R3178	T12798	T12797	pobj	lacZ,of
R3179	T12799	T12800	punct	(,N
R3180	T12800	T12780	parataxis	N,activate
R3181	T12801	T12802	compound	Fig.,4B
R3182	T12802	T12800	dep	4B,N
R3183	T12803	T12800	punct	", ",N
R3184	T12804	T12800	amod	full,N
R3185	T12805	T12800	punct	×,N
R3186	T12806	T12800	punct	),N
R3187	T12807	T12780	punct	.,activate
R3188	T12809	T12810	det	The,stretch
R3189	T12810	T12816	nsubj	stretch,was
R3190	T12811	T12812	nmod	N,terminus
R3191	T12812	T12810	nmod	terminus,stretch
R3192	T12813	T12812	punct	-,terminus
R3193	T12814	T12815	nummod	400,aa
R3194	T12815	T12810	compound	aa,stretch
R3195	T12817	T12810	prep	of,stretch
R3196	T12818	T12819	compound	mr,s
R3197	T12819	T12817	pobj	s,of
R3198	T12820	T12819	punct	-,s
R3199	T12821	T12816	acomp	able,was
R3200	T12822	T12823	aux	to,induce
R3201	T12823	T12821	xcomp	induce,able
R3202	T12824	T12823	dobj	transcription,induce
R3203	T12825	T12824	prep	of,transcription
R3204	T12826	T12825	pobj	lacZ,of
R3205	T12827	T12823	advmod	weakly,induce
R3206	T12828	T12823	prep	with,induce
R3207	T12829	T12830	det	the,stretch
R3208	T12830	T12828	pobj	stretch,with
R3209	T12831	T12830	amod	same,stretch
R3210	T12832	T12833	nmod	N,terminus
R3211	T12833	T12830	nmod	terminus,stretch
R3212	T12834	T12833	punct	-,terminus
R3213	T12835	T12836	nummod	400,aa
R3214	T12836	T12830	compound	aa,stretch
R3215	T12837	T12830	prep	of,stretch
R3216	T12838	T12839	compound	mr,s
R3217	T12839	T12837	pobj	s,of
R3218	T12840	T12839	punct	-,s
R3219	T12841	T12842	punct	(,N
R3220	T12842	T12816	parataxis	N,was
R3221	T12843	T12844	compound	Fig.,4B
R3222	T12844	T12842	dep	4B,N
R3223	T12845	T12842	punct	", ",N
R3224	T12846	T12842	nmod	N,N
R3225	T12847	T12842	punct	×,N
R3226	T12848	T12842	punct	),N
R3227	T12849	T12816	punct	.,was
R3228	T12851	T12852	mark	Although,activates
R3229	T12852	T12864	advcl	activates,observed
R3230	T12853	T12854	det	the,protein
R3231	T12854	T12852	nsubj	protein,activates
R3232	T12855	T12856	nmod	N,terminus
R3233	T12856	T12854	nmod	terminus,protein
R3234	T12857	T12856	punct	-,terminus
R3235	T12858	T12859	nummod	400,aa
R3236	T12859	T12854	compound	aa,protein
R3237	T12860	T12861	compound	mr,s
R3238	T12861	T12854	compound	s,protein
R3239	T12862	T12861	punct	-,s
R3240	T12863	T12852	advmod	weakly,activates
R3241	T12865	T12866	compound	lacZ,transcription
R3242	T12866	T12852	dobj	transcription,activates
R3243	T12867	T12852	prep	with,activates
R3244	T12868	T12869	det	the,portion
R3245	T12869	T12867	pobj	portion,with
R3246	T12870	T12869	amod	same,portion
R3247	T12871	T12872	compound	N,terminus
R3248	T12872	T12869	compound	terminus,portion
R3249	T12873	T12872	punct	-,terminus
R3250	T12874	T12864	punct	", ",observed
R3251	T12875	T12876	det	a,activation
R3252	T12876	T12864	nsubjpass	activation,observed
R3253	T12877	T12878	advmod	much,stronger
R3254	T12878	T12876	amod	stronger,activation
R3255	T12879	T12876	prep	of,activation
R3256	T12880	T12881	compound	lacZ,expression
R3257	T12881	T12879	pobj	expression,of
R3258	T12882	T12864	auxpass	was,observed
R3259	T12883	T12864	prep	with,observed
R3260	T12884	T12885	det	a,portion
R3261	T12885	T12883	pobj	portion,with
R3262	T12886	T12887	compound	C,terminus
R3263	T12887	T12885	compound	terminus,portion
R3264	T12888	T12887	punct	-,terminus
R3265	T12889	T12885	acl	encoding,portion
R3266	T12890	T12891	det	the,stretch
R3267	T12891	T12889	dobj	stretch,encoding
R3268	T12892	T12893	nummod	391,aa
R3269	T12893	T12891	compound	aa,stretch
R3270	T12894	T12895	punct	–,542
R3271	T12895	T12892	prep	542,391
R3272	T12896	T12891	prep	of,stretch
R3273	T12897	T12898	compound	mr,s
R3274	T12898	T12896	pobj	s,of
R3275	T12899	T12898	punct	-,s
R3276	T12900	T12901	punct	(,C
R3277	T12901	T12864	parataxis	C,observed
R3278	T12902	T12903	compound	Fig.,4B
R3279	T12903	T12901	dep	4B,C
R3280	T12904	T12901	punct	", ",C
R3281	T12905	T12901	amod	full,C
R3282	T12906	T12901	punct	×,C
R3283	T12907	T12901	punct	", ",C
R3284	T12908	T12909	nmod	C,C
R3285	T12909	T12901	appos	C,C
R3286	T12910	T12909	punct	×,C
R3287	T12911	T12901	punct	),C
R3288	T12912	T12864	punct	.,observed
R3289	T12914	T12915	poss	Our,assay
R3290	T12915	T12917	nsubj	assay,indicated
R3291	T12916	T12915	compound	GAL4,assay
R3292	T12918	T12919	mark	that,was
R3293	T12919	T12917	ccomp	was,indicated
R3294	T12920	T12921	det	the,signal
R3295	T12921	T12919	nsubj	signal,was
R3296	T12922	T12923	advmod	when,was
R3297	T12923	T12921	relcl	was,signal
R3298	T12924	T12925	det	the,s
R3299	T12925	T12923	nsubj	s,was
R3300	T12926	T12927	amod	full,length
R3301	T12927	T12925	compound	length,s
R3302	T12928	T12927	punct	-,length
R3303	T12929	T12925	compound	mr,s
R3304	T12930	T12925	punct	-,s
R3305	T12931	T12923	acomp	present,was
R3306	T12932	T12923	prep	in,was
R3307	T12933	T12934	preconj	both,contexts
R3308	T12934	T12932	pobj	contexts,in
R3309	T12935	T12934	det	the,contexts
R3310	T12936	T12934	nmod	bait,contexts
R3311	T12937	T12936	cc	and,bait
R3312	T12938	T12936	conj	prey,bait
R3313	T12939	T12919	acomp	weaker,was
R3314	T12940	T12939	prep	than,weaker
R3315	T12941	T12942	advmod	when,used
R3316	T12942	T12940	pcomp	used,than
R3317	T12943	T12944	amod	isolated,domains
R3318	T12944	T12942	nsubjpass	domains,used
R3319	T12945	T12944	compound	SAM,domains
R3320	T12946	T12942	auxpass	were,used
R3321	T12947	T12917	punct	.,indicated
R3322	T12949	T12950	nsubj	This,reflect
R3323	T12951	T12950	aux	may,reflect
R3324	T12952	T12950	advmod	simply,reflect
R3325	T12953	T12954	det	the,tendency
R3326	T12954	T12950	dobj	tendency,reflect
R3327	T12955	T12956	mark	for,enter
R3328	T12956	T12954	advcl	enter,tendency
R3329	T12957	T12958	det	the,proteins
R3330	T12958	T12956	nsubj	proteins,enter
R3331	T12959	T12958	amod	small,proteins
R3332	T12960	T12958	compound	fusion,proteins
R3333	T12961	T12956	aux	to,enter
R3334	T12962	T12963	det	the,nucleus
R3335	T12963	T12956	dobj	nucleus,enter
R3336	T12964	T12963	compound	yeast,nucleus
R3337	T12965	T12956	cc	and,enter
R3338	T12966	T12956	conj	occupy,enter
R3339	T12967	T12968	compound	GAL4,binding
R3340	T12968	T12969	compound	binding,sites
R3341	T12969	T12966	dobj	sites,occupy
R3342	T12970	T12950	punct	.,reflect
R3343	T12972	T12973	advmod	Alternatively,be
R3344	T12974	T12973	punct	", ",be
R3345	T12975	T12976	det	the,domain
R3346	T12976	T12973	nsubj	domain,be
R3347	T12977	T12976	compound	SAM,domain
R3348	T12978	T12973	aux	may,be
R3349	T12979	T12980	advmod	less,accessible
R3350	T12980	T12973	acomp	accessible,be
R3351	T12981	T12980	prep	for,accessible
R3352	T12982	T12981	pobj	interaction,for
R3353	T12983	T12973	prep	in,be
R3354	T12984	T12985	det	the,context
R3355	T12985	T12983	pobj	context,in
R3356	T12986	T12987	amod	full,length
R3357	T12987	T12985	compound	length,context
R3358	T12988	T12987	punct	-,length
R3359	T12989	T12985	compound	protein,context
R3360	T12990	T12991	mark	as,reported
R3361	T12991	T12973	advcl	reported,be
R3362	T12992	T12991	advmod	previously,reported
R3363	T12993	T12994	punct	[,39
R3364	T12994	T12973	parataxis	39,be
R3365	T12995	T12994	punct	],39
R3366	T12996	T12973	punct	.,be
R3367	T12999	T13000	aux	To,confirm
R3368	T13000	T13001	advcl	confirm,performed
R3369	T13002	T13003	compound	self,association
R3370	T13003	T13000	dobj	association,confirm
R3371	T13004	T13003	punct	-,association
R3372	T13005	T13003	prep	of,association
R3373	T13006	T13007	det	the,protein
R3374	T13007	T13005	pobj	protein,of
R3375	T13008	T13009	compound	mr,s
R3376	T13009	T13007	compound	s,protein
R3377	T13010	T13009	punct	-,s
R3378	T13011	T13000	prep	in,confirm
R3379	T13012	T13013	amod	mammalian,cells
R3380	T13013	T13011	pobj	cells,in
R3381	T13014	T13001	punct	", ",performed
R3382	T13015	T13001	nsubj	we,performed
R3383	T13016	T13001	advmod	next,performed
R3384	T13017	T13018	compound	co-immunoprecipitation,studies
R3385	T13018	T13001	dobj	studies,performed
R3386	T13019	T13001	prep	in,performed
R3387	T13020	T13021	compound	HEK293T,cells
R3388	T13021	T13019	pobj	cells,in
R3389	T13022	T13001	prep	by,performed
R3390	T13023	T13022	pobj	co-transfection,by
R3391	T13024	T13023	prep	of,co-transfection
R3392	T13025	T13026	npadvmod	HA,tagged
R3393	T13026	T13028	amod	tagged,s
R3394	T13027	T13026	punct	-,tagged
R3395	T13028	T13024	pobj	s,of
R3396	T13029	T13030	amod	full,length
R3397	T13030	T13032	dep	length,truncated
R3398	T13031	T13030	punct	-,length
R3399	T13032	T13028	amod	truncated,s
R3400	T13033	T13032	punct	/,truncated
R3401	T13034	T13028	compound	mr,s
R3402	T13035	T13028	punct	-,s
R3403	T13036	T13028	cc	and,s
R3404	T13037	T13038	npadvmod	Flag,tagged
R3405	T13038	T13040	amod	tagged,s
R3406	T13039	T13038	punct	-,tagged
R3407	T13040	T13028	conj	s,s
R3408	T13041	T13042	amod	full,length
R3409	T13042	T13044	dep	length,truncated
R3410	T13043	T13042	punct	-,length
R3411	T13044	T13040	amod	truncated,s
R3412	T13045	T13044	punct	/,truncated
R3413	T13046	T13040	compound	mr,s
R3414	T13047	T13040	punct	-,s
R3415	T13048	T13049	punct	(,5A
R3416	T13049	T13001	parataxis	5A,performed
R3417	T13050	T13049	compound	Fig.,5A
R3418	T13051	T13049	punct	),5A
R3419	T13052	T13001	punct	.,performed
R3420	T13054	T13055	prep	As,constructed
R3421	T13056	T13057	det	a,control
R3422	T13057	T13054	pobj	control,As
R3423	T13058	T13057	amod	negative,control
R3424	T13059	T13055	punct	", ",constructed
R3425	T13060	T13055	nsubj	we,constructed
R3426	T13061	T13062	npadvmod	Flag,tagged
R3427	T13062	T13064	amod	tagged,hedgehog
R3428	T13063	T13062	punct	-,tagged
R3429	T13064	T13055	dobj	hedgehog,constructed
R3430	T13065	T13064	amod	Sonic,hedgehog
R3431	T13066	T13064	punct	(,hedgehog
R3432	T13067	T13064	appos	Shh,hedgehog
R3433	T13068	T13055	punct	),constructed
R3434	T13069	T13070	punct	(,2
R3435	T13070	T13055	parataxis	2,constructed
R3436	T13071	T13070	nmod	lane,2
R3437	T13072	T13070	cc	and,2
R3438	T13073	T13070	conj	7,2
R3439	T13074	T13070	punct	),2
R3440	T13075	T13055	punct	.,constructed
R3441	T13077	T13078	prep	In,co-immunoprecipitated
R3442	T13079	T13077	pobj	accordance,In
R3443	T13080	T13079	prep	with,accordance
R3444	T13081	T13082	det	the,result
R3445	T13082	T13080	pobj	result,with
R3446	T13083	T13082	prep	of,result
R3447	T13084	T13085	det	the,assay
R3448	T13085	T13083	pobj	assay,of
R3449	T13086	T13085	nmod	yeast,assay
R3450	T13087	T13088	nummod	two,hybrid
R3451	T13088	T13085	compound	hybrid,assay
R3452	T13089	T13088	punct	-,hybrid
R3453	T13090	T13085	compound	GAL4,assay
R3454	T13091	T13078	punct	", ",co-immunoprecipitated
R3455	T13092	T13093	npadvmod	HA,tagged
R3456	T13093	T13095	amod	tagged,s
R3457	T13094	T13093	punct	-,tagged
R3458	T13095	T13078	nsubjpass	s,co-immunoprecipitated
R3459	T13096	T13097	amod	full,length
R3460	T13097	T13095	compound	length,s
R3461	T13098	T13097	punct	-,length
R3462	T13099	T13095	compound	mr,s
R3463	T13100	T13095	punct	-,s
R3464	T13101	T13095	punct	(,s
R3465	T13102	T13103	amod	full,HA
R3466	T13103	T13095	appos	HA,s
R3467	T13104	T13103	punct	-,HA
R3468	T13105	T13078	punct	),co-immunoprecipitated
R3469	T13106	T13078	auxpass	was,co-immunoprecipitated
R3470	T13107	T13078	prep	with,co-immunoprecipitated
R3471	T13108	T13109	npadvmod	Flag,tagged
R3472	T13109	T13111	amod	tagged,s
R3473	T13110	T13109	punct	-,tagged
R3474	T13111	T13107	pobj	s,with
R3475	T13112	T13113	amod	full,length
R3476	T13113	T13111	compound	length,s
R3477	T13114	T13113	punct	-,length
R3478	T13115	T13111	compound	mr,s
R3479	T13116	T13111	punct	-,s
R3480	T13117	T13111	punct	(,s
R3481	T13118	T13119	compound	Flag,mrs
R3482	T13119	T13111	appos	mrs,s
R3483	T13120	T13119	punct	-,mrs
R3484	T13121	T13111	punct	),s
R3485	T13122	T13111	cc	and,s
R3486	T13123	T13124	det	the,portion
R3487	T13124	T13111	conj	portion,s
R3488	T13125	T13126	npadvmod	Flag,tagged
R3489	T13126	T13124	amod	tagged,portion
R3490	T13127	T13126	punct	-,tagged
R3491	T13128	T13129	compound	C,terminus
R3492	T13129	T13124	compound	terminus,portion
R3493	T13130	T13129	punct	-,terminus
R3494	T13131	T13124	acl	containing,portion
R3495	T13132	T13133	det	the,domain
R3496	T13133	T13131	dobj	domain,containing
R3497	T13134	T13133	compound	SAM,domain
R3498	T13135	T13124	punct	(,portion
R3499	T13136	T13137	compound	Flag,SAM
R3500	T13137	T13124	appos	SAM,portion
R3501	T13138	T13137	punct	-,SAM
R3502	T13139	T13078	punct	),co-immunoprecipitated
R3503	T13140	T13078	punct	", ",co-immunoprecipitated
R3504	T13141	T13078	advmod	respectively,co-immunoprecipitated
R3505	T13142	T13143	punct	(,lane3
R3506	T13143	T13078	parataxis	lane3,co-immunoprecipitated
R3507	T13144	T13145	compound	Fig,5B
R3508	T13145	T13143	dep	5B,lane3
R3509	T13146	T13143	punct	", ",lane3
R3510	T13147	T13143	cc	and,lane3
R3511	T13148	T13143	conj	lane5,lane3
R3512	T13149	T13143	punct	),lane3
R3513	T13150	T13078	punct	.,co-immunoprecipitated
R3514	T13152	T13153	nsubj	We,found
R3515	T13154	T13153	advmod	also,found
R3516	T13155	T13156	det	a,band
R3517	T13156	T13153	dobj	band,found
R3518	T13157	T13156	amod	weak,band
R3519	T13158	T13156	compound	co-immunoprecipitation,band
R3520	T13159	T13153	prep	in,found
R3521	T13160	T13159	pobj	co-transfection,in
R3522	T13161	T13160	prep	of,co-transfection
R3523	T13162	T13163	amod	full,HA
R3524	T13163	T13161	pobj	HA,of
R3525	T13164	T13163	punct	-,HA
R3526	T13165	T13163	cc	and,HA
R3527	T13166	T13167	npadvmod	Flag,tagged
R3528	T13167	T13169	amod	tagged,portion
R3529	T13236	T13234	pobj	SAM,with
R3530	T13168	T13167	punct	-,tagged
R3531	T13237	T13236	punct	-,SAM
R3532	T13238	T13239	punct	(,5B
R3533	T13169	T13163	conj	portion,HA
R3534	T13239	T13228	parataxis	5B,co-immunoprecipitated
R3535	T13240	T13239	compound	Fig.,5B
R3536	T13170	T13171	compound	N,terminus
R3537	T13241	T13239	punct	", ",5B
R3538	T13242	T13239	appos	lane,5B
R3539	T13243	T13242	nummod	10,lane
R3540	T13171	T13169	compound	terminus,portion
R3541	T13244	T13239	punct	),5B
R3542	T13245	T13202	punct	.,co-immunoprecipitated
R3543	T13172	T13171	punct	-,terminus
R3544	T13173	T13169	prep	of,portion
R3545	T13174	T13175	compound	mr,s
R3546	T13175	T13173	pobj	s,of
R3547	T13176	T13175	punct	-,s
R3548	T13177	T13169	punct	(,portion
R3549	T13247	T13248	aux	To,investigate
R3550	T13178	T13179	compound	Flag,ΔSAM
R3551	T13248	T13249	advcl	investigate,generated
R3552	T13179	T13169	appos	ΔSAM,portion
R3553	T13250	T13251	mark	whether,associates
R3554	T13251	T13248	ccomp	associates,investigate
R3555	T13180	T13179	punct	-,ΔSAM
R3556	T13252	T13253	det	the,protein
R3557	T13253	T13251	nsubj	protein,associates
R3558	T13254	T13255	compound	mr,s
R3559	T13181	T13182	punct	", ",lane
R3560	T13255	T13253	compound	s,protein
R3561	T13256	T13255	punct	-,s
R3562	T13182	T13153	parataxis	lane,found
R3563	T13257	T13251	dep	self,associates
R3564	T13258	T13251	punct	-,associates
R3565	T13259	T13251	advmod	mainly,associates
R3566	T13183	T13184	compound	Fig.,5B
R3567	T13260	T13251	prep	through,associates
R3568	T13261	T13262	det	the,domain
R3569	T13262	T13260	pobj	domain,through
R3570	T13184	T13182	dep	5B,lane
R3571	T13263	T13262	compound	SAM,domain
R3572	T13264	T13249	punct	", ",generated
R3573	T13185	T13182	punct	", ",lane
R3574	T13265	T13266	nummod	two,mutations
R3575	T13266	T13249	nsubjpass	mutations,generated
R3576	T13267	T13268	npadvmod	site,directed
R3577	T13186	T13182	nummod	4,lane
R3578	T13268	T13266	amod	directed,mutations
R3579	T13269	T13268	punct	-,directed
R3580	T13270	T13249	auxpass	were,generated
R3581	T13187	T13182	punct	),lane
R3582	T13271	T13249	prep	in,generated
R3583	T13272	T13273	det	the,domain
R3584	T13273	T13271	pobj	domain,in
R3585	T13188	T13153	punct	.,found
R3586	T13274	T13273	compound	SAM,domain
R3587	T13275	T13273	prep	of,domain
R3588	T13276	T13277	compound	mr,s
R3589	T13190	T13191	advmod	When,co-transfected
R3590	T13191	T13202	advcl	co-transfected,co-immunoprecipitated
R3591	T13277	T13275	pobj	s,of
R3592	T13192	T13193	compound	ΔSAM,HA
R3593	T13278	T13277	punct	-,s
R3594	T13279	T13280	punct	(,arrows
R3595	T13280	T13249	parataxis	arrows,generated
R3596	T13281	T13282	compound	Fig.,1B
R3597	T13282	T13280	dep	1B,arrows
R3598	T13193	T13191	nsubjpass	HA,co-transfected
R3599	T13283	T13280	punct	", ",arrows
R3600	T13194	T13193	punct	-,HA
R3601	T13195	T13193	cc	and,HA
R3602	T13284	T13280	punct	),arrows
R3603	T13196	T13197	npadvmod	Flag,tagged
R3604	T13285	T13249	punct	.,generated
R3605	T13197	T13199	amod	tagged,mutants
R3606	T13287	T13288	det	These,mutations
R3607	T13288	T13289	nsubj	mutations,alter
R3608	T13198	T13197	punct	-,tagged
R3609	T13290	T13289	dobj	residues,alter
R3610	T13291	T13292	dep	that,conserved
R3611	T13199	T13193	conj	mutants,HA
R3612	T13292	T13290	relcl	conserved,residues
R3613	T13293	T13292	auxpass	are,conserved
R3614	T13294	T13292	prep	in,conserved
R3615	T13200	T13199	compound	deletion,mutants
R3616	T13295	T13296	det	the,domain
R3617	T13296	T13294	pobj	domain,in
R3618	T13297	T13296	compound	SAM,domain
R3619	T13201	T13191	auxpass	were,co-transfected
R3620	T13298	T13296	prep	of,domain
R3621	T13299	T13298	pobj	ph,of
R3622	T13300	T13289	cc	and,alter
R3623	T13203	T13202	punct	", ",co-immunoprecipitated
R3624	T13301	T13302	amod	previous,report
R3625	T13302	T13303	nsubj	report,indicates
R3626	T13303	T13289	conj	indicates,alter
R3627	T13304	T13305	mark	that,cause
R3628	T13204	T13205	compound	ΔSAM,HA
R3629	T13305	T13303	ccomp	cause,indicates
R3630	T13306	T13307	det	these,mutations
R3631	T13205	T13202	nsubjpass	HA,co-immunoprecipitated
R3632	T13307	T13305	nsubj	mutations,cause
R3633	T13308	T13307	prep	of,mutations
R3634	T13206	T13205	punct	-,HA
R3635	T13309	T13310	compound	ph,SAM
R3636	T13310	T13308	pobj	SAM,of
R3637	T13311	T13310	punct	-,SAM
R3638	T13207	T13202	auxpass	was,co-immunoprecipitated
R3639	T13312	T13313	amod	significant,reduction
R3640	T13313	T13305	dobj	reduction,cause
R3641	T13314	T13313	prep	in,reduction
R3642	T13208	T13202	prep	with,co-immunoprecipitated
R3643	T13315	T13316	compound	binding,activity
R3644	T13316	T13314	pobj	activity,in
R3645	T13317	T13316	prep	to,activity
R3646	T13209	T13210	compound	Flag,mrs
R3647	T13318	T13319	det	the,protein
R3648	T13319	T13317	pobj	protein,to
R3649	T13320	T13319	amod	other,protein
R3650	T13321	T13322	compound	SAM,domain
R3651	T13322	T13323	npadvmod	domain,containing
R3652	T13210	T13208	pobj	mrs,with
R3653	T13323	T13319	amod	containing,protein
R3654	T13324	T13323	punct	-,containing
R3655	T13325	T13319	punct	", ",protein
R3656	T13326	T13327	compound	Sex,comb
R3657	T13211	T13210	punct	-,mrs
R3658	T13327	T13319	appos	comb,protein
R3659	T13328	T13327	prep	on,comb
R3660	T13329	T13328	pobj	midleg,on
R3661	T13212	T13210	cc	and,mrs
R3662	T13330	T13327	punct	(,comb
R3663	T13331	T13327	appos	Scm,comb
R3664	T13213	T13214	compound	Flag,ΔSAM
R3665	T13332	T13305	punct	),cause
R3666	T13333	T13334	punct	(,41
R3667	T13334	T13305	parataxis	41,cause
R3668	T13214	T13210	conj	ΔSAM,mrs
R3669	T13335	T13334	punct	),41
R3670	T13336	T13303	punct	.,indicates
R3671	T13215	T13214	punct	-,ΔSAM
R3672	T13338	T13339	prep	Based,introduced
R3673	T13216	T13217	punct	(,5B
R3674	T13340	T13338	prep	on,Based
R3675	T13341	T13342	det	this,result
R3676	T13342	T13340	pobj	result,on
R3677	T13217	T13202	parataxis	5B,co-immunoprecipitated
R3678	T13343	T13339	punct	", ",introduced
R3679	T13344	T13339	nsubj	we,introduced
R3680	T13345	T13346	nummod	two,types
R3681	T13218	T13217	compound	Fig.,5B
R3682	T13346	T13339	dobj	types,introduced
R3683	T13347	T13346	prep	of,types
R3684	T13348	T13349	npadvmod	site,directed
R3685	T13219	T13217	punct	", ",5B
R3686	T13349	T13351	amod	directed,mutations
R3687	T13350	T13349	punct	-,directed
R3688	T13220	T13217	appos	lane,5B
R3689	T13351	T13347	pobj	mutations,of
R3690	T13352	T13346	punct	", ",types
R3691	T13221	T13220	nummod	8,lane
R3692	T13353	T13354	dep	which,correspond
R3693	T13354	T13346	relcl	correspond,types
R3694	T13355	T13354	prep	to,correspond
R3695	T13222	T13220	cc	and,lane
R3696	T13356	T13357	det	the,mutations
R3697	T13357	T13355	pobj	mutations,to
R3698	T13358	T13357	acl	introduced,mutations
R3699	T13223	T13220	conj	lane,lane
R3700	T13359	T13358	prep	in,introduced
R3701	T13360	T13361	compound	ph,protein
R3702	T13361	T13359	pobj	protein,in
R3703	T13224	T13223	nummod	9,lane
R3704	T13362	T13358	punct	", ",introduced
R3705	T13225	T13217	punct	),5B
R3706	T13363	T13358	prep	into,introduced
R3707	T13364	T13365	npadvmod	Flag,tagged
R3708	T13365	T13367	amod	tagged,s
R3709	T13226	T13202	punct	", ",co-immunoprecipitated
R3710	T13366	T13365	punct	-,tagged
R3711	T13367	T13363	pobj	s,into
R3712	T13227	T13228	mark	while,co-immunoprecipitated
R3713	T13368	T13369	amod	full,length
R3714	T13369	T13367	compound	length,s
R3715	T13370	T13369	punct	-,length
R3716	T13228	T13202	advcl	co-immunoprecipitated,co-immunoprecipitated
R3717	T13371	T13367	compound	mr,s
R3718	T13372	T13367	punct	-,s
R3719	T13373	T13346	punct	(,types
R3720	T13229	T13230	compound	ΔSAM,HA
R3721	T13374	T13375	compound	Flag,W404A
R3722	T13375	T13346	appos	W404A,types
R3723	T13376	T13375	punct	-,W404A
R3724	T13230	T13228	nsubjpass	HA,co-immunoprecipitated
R3725	T13377	T13375	cc	and,W404A
R3726	T13378	T13379	compound	Flag,G453A
R3727	T13379	T13375	conj	G453A,W404A
R3728	T13231	T13230	punct	-,HA
R3729	T13380	T13379	punct	-,G453A
R3730	T13381	T13339	punct	),introduced
R3731	T13382	T13339	punct	.,introduced
R3732	T13232	T13228	auxpass	was,co-immunoprecipitated
R3733	T13384	T13385	nsubj	We,found
R3734	T13233	T13228	neg	not,co-immunoprecipitated
R3735	T13234	T13228	prep	with,co-immunoprecipitated
R3736	T13386	T13387	mark	that,reduced
R3737	T13387	T13385	ccomp	reduced,found
R3738	T13388	T13389	compound	Flag,W404A
R3739	T13235	T13236	compound	Flag,SAM
R3740	T13389	T13391	compound	W404A,binding
R3741	T13390	T13389	punct	-,W404A
R3742	T13391	T13392	compound	binding,activity
R3743	T13392	T13387	nsubjpass	activity,reduced
R3744	T13393	T13387	auxpass	was,reduced
R3745	T13394	T13387	advmod	significantly,reduced
R3746	T13395	T13385	cc	and,found
R3747	T13396	T13397	compound	Flag,G453A
R3748	T13397	T13399	compound	G453A,binding
R3749	T13398	T13397	punct	-,G453A
R3750	T13399	T13400	compound	binding,activity
R3751	T13400	T13401	nsubjpass	activity,reduced
R3752	T13401	T13385	conj	reduced,found
R3753	T13402	T13401	auxpass	was,reduced
R3754	T13403	T13401	advmod	also,reduced
R3755	T13404	T13401	advmod	slightly,reduced
R3756	T13405	T13401	prep	compared,reduced
R3757	T13406	T13405	prep	to,compared
R3758	T13407	T13408	compound	Flag,mrs
R3759	T13408	T13406	pobj	mrs,to
R3760	T13409	T13408	punct	-,mrs
R3761	T13410	T13411	punct	(,5C
R3762	T13411	T13401	parataxis	5C,reduced
R3763	T13412	T13411	compound	Fig.,5C
R3764	T13413	T13411	punct	),5C
R3765	T13414	T13401	punct	.,reduced
R3766	T13416	T13417	det	These,results
R3767	T13417	T13418	nsubj	results,indicate
R3768	T13419	T13418	punct	", ",indicate
R3769	T13420	T13418	advmod	together,indicate
R3770	T13421	T13420	prep	with,together
R3771	T13422	T13423	nmod	yeast,assay
R3772	T13423	T13421	pobj	assay,with
R3773	T13424	T13425	nummod	two,hybrid
R3774	T13425	T13423	compound	hybrid,assay
R3775	T13426	T13425	punct	-,hybrid
R3776	T13427	T13423	compound	GAL4,assay
R3777	T13428	T13418	punct	", ",indicate
R3778	T13429	T13430	mark	that,associates
R3779	T13430	T13418	ccomp	associates,indicate
R3780	T13431	T13432	det	the,protein
R3781	T13432	T13430	nsubj	protein,associates
R3782	T13433	T13434	compound	mr,s
R3783	T13434	T13432	compound	s,protein
R3784	T13435	T13434	punct	-,s
R3785	T13436	T13430	dep	self,associates
R3786	T13437	T13430	punct	-,associates
R3787	T13438	T13430	advmod	strongly,associates
R3788	T13439	T13430	prep	through,associates
R3789	T13440	T13441	poss	its,domain
R3790	T13441	T13439	pobj	domain,through
R3791	T13442	T13441	compound	SAM,domain
R3792	T13443	T13430	cc	and,associates
R3793	T13444	T13430	conj	weakly,associates
R3794	T13445	T13444	prep	through,weakly
R3795	T13446	T13447	det	the,portion
R3796	T13447	T13445	pobj	portion,through
R3797	T13448	T13449	compound	N,terminus
R3798	T13449	T13447	compound	terminus,portion
R3799	T13450	T13449	punct	-,terminus
R3800	T13451	T13447	acl	lacking,portion
R3801	T13452	T13453	compound	SAM,domain
R3802	T13453	T13451	dobj	domain,lacking
R3803	T13454	T13418	punct	.,indicate
R3806	T13863	T13864	det	The,localization
R3807	T13865	T13864	amod	subcellular,localization
R3808	T13866	T13864	prep	of,localization
R3809	T13867	T13868	compound	mr,s
R3810	T13868	T13870	compound	s,protein
R3811	T13869	T13868	punct	-,s
R3812	T13870	T13866	pobj	protein,of
R3813	T13871	T13864	prep	in,localization
R3814	T13872	T13873	amod	mammalian,cells
R3815	T13873	T13871	pobj	cells,in
R3816	T13875	T13876	det	The,signal
R3817	T13876	T13880	nsubj	signal,suggests
R3818	T13877	T13876	amod	putative,signal
R3819	T13878	T13879	amod	nuclear,localization
R3820	T13879	T13876	compound	localization,signal
R3821	T13881	T13876	prep	at,signal
R3822	T13882	T13883	det	the,terminus
R3823	T13883	T13881	pobj	terminus,at
R3824	T13884	T13883	compound	N,terminus
R3825	T13885	T13883	punct	-,terminus
R3826	T13886	T13883	prep	of,terminus
R3827	T13887	T13888	det	the,protein
R3828	T13888	T13886	pobj	protein,of
R3829	T13889	T13890	compound	mr,s
R3830	T13890	T13888	compound	s,protein
R3831	T13891	T13890	punct	-,s
R3832	T13892	T13893	punct	(,box
R3833	T13893	T13876	parataxis	box,signal
R3834	T13894	T13895	compound	Fig.,1A
R3835	T13895	T13893	dep	1A,box
R3836	T13896	T13893	punct	", ",box
R3837	T13897	T13893	amod	dashed,box
R3838	T13898	T13893	punct	),box
R3839	T13899	T13900	mark	that,localizes
R3840	T13900	T13880	ccomp	localizes,suggests
R3841	T13901	T13902	det	the,protein
R3842	T13902	T13900	nsubj	protein,localizes
R3843	T13903	T13904	compound	mr,s
R3844	T13904	T13902	compound	s,protein
R3845	T13905	T13904	punct	-,s
R3846	T13906	T13900	prep	in,localizes
R3847	T13907	T13908	det	the,nucleus
R3848	T13908	T13906	pobj	nucleus,in
R3849	T13909	T13880	punct	.,suggests
R3850	T13911	T13912	aux	To,determine
R3851	T13912	T13913	advcl	determine,introduced
R3852	T13914	T13915	det	the,localization
R3853	T13915	T13912	dobj	localization,determine
R3854	T13916	T13915	amod	subcellular,localization
R3855	T13917	T13915	prep	of,localization
R3856	T13918	T13919	compound	mr,s
R3857	T13919	T13917	pobj	s,of
R3858	T13920	T13919	punct	-,s
R3859	T13921	T13912	prep	in,determine
R3860	T13922	T13923	amod	mammalian,cells
R3861	T13923	T13921	pobj	cells,in
R3862	T13924	T13913	punct	", ",introduced
R3863	T13925	T13913	nsubj	we,introduced
R3864	T13926	T13927	det	an,plasmid
R3865	T13927	T13913	dobj	plasmid,introduced
R3866	T13928	T13929	npadvmod	HA,tagged
R3867	T13929	T13927	amod	tagged,plasmid
R3868	T13930	T13929	punct	-,tagged
R3869	T13931	T13932	amod	full,length
R3870	T13932	T13927	compound	length,plasmid
R3871	T13933	T13932	punct	-,length
R3872	T13934	T13935	compound	mr,s
R3873	T13935	T13927	compound	s,plasmid
R3874	T13936	T13935	punct	-,s
R3875	T13937	T13913	prep	into,introduced
R3876	T13938	T13939	compound	HEK293T,cells
R3877	T13939	T13937	pobj	cells,into
R3878	T13940	T13913	punct	.,introduced
R3879	T13942	T13943	amod	Confocal,microscopy
R3880	T13943	T13944	nsubj	microscopy,showed
R3881	T13945	T13946	mark	that,localized
R3882	T13946	T13944	ccomp	localized,showed
R3883	T13947	T13948	det	the,protein
R3884	T13948	T13946	nsubj	protein,localized
R3885	T13949	T13950	compound	mr,s
R3886	T13950	T13948	compound	s,protein
R3887	T13951	T13950	punct	-,s
R3888	T13952	T13953	advmod	mainly,in
R3889	T13953	T13946	prep	in,localized
R3890	T13954	T13955	det	the,nucleus
R3891	T13955	T13953	pobj	nucleus,in
R3892	T13956	T13955	prep	of,nucleus
R3893	T13957	T13958	compound	HEK293T,cells
R3894	T13958	T13956	pobj	cells,of
R3895	T13959	T13960	punct	(,B
R3896	T13960	T13944	parataxis	B,showed
R3897	T13961	T13960	nmod	Fig.,B
R3898	T13962	T13960	nmod	6A,B
R3899	T13963	T13960	punct	", ",B
R3900	T13964	T13960	punct	),B
R3901	T13965	T13944	punct	.,showed
R3902	T13967	T13968	aux	To,confirm
R3903	T13968	T13969	advcl	confirm,co-immunostained
R3904	T13970	T13971	det	the,localization
R3905	T13971	T13968	dobj	localization,confirm
R3906	T13972	T13971	amod	precise,localization
R3907	T13973	T13971	prep	of,localization
R3908	T13974	T13975	det	the,protein
R3909	T13975	T13973	pobj	protein,of
R3910	T13976	T13977	compound	mr,s
R3911	T13977	T13975	compound	s,protein
R3912	T13978	T13977	punct	-,s
R3913	T13979	T13969	punct	", ",co-immunostained
R3914	T13980	T13981	amod	full,length
R3915	T13981	T13983	compound	length,s
R3916	T13982	T13981	punct	-,length
R3917	T13983	T13969	nsubjpass	s,co-immunostained
R3918	T13984	T13983	compound	mr,s
R3919	T13985	T13983	punct	-,s
R3920	T13986	T13969	auxpass	was,co-immunostained
R3921	T13987	T13969	prep	with,co-immunostained
R3922	T13988	T13987	pobj	DAPI,with
R3923	T13989	T13990	punct	(,6C
R3924	T13990	T13969	parataxis	6C,co-immunostained
R3925	T13991	T13990	compound	Fig.,6C
R3926	T13992	T13993	punct	–,E
R3927	T13993	T13990	prep	E,6C
R3928	T13994	T13990	punct	),6C
R3929	T13995	T13969	punct	.,co-immunostained
R3930	T13997	T13998	det	These,data
R3931	T13998	T13999	nsubj	data,showed
R3932	T14000	T14001	mark	that,localizes
R3933	T14001	T13999	ccomp	localizes,showed
R3934	T14002	T14003	det	the,protein
R3935	T14003	T14001	nsubj	protein,localizes
R3936	T14004	T14005	compound	mr,s
R3937	T14005	T14003	compound	s,protein
R3938	T14006	T14005	punct	-,s
R3939	T14007	T14001	advmod	preferentially,localizes
R3940	T14008	T14001	prep	to,localizes
R3941	T14009	T14010	det	the,nucleus
R3942	T14010	T14008	pobj	nucleus,to
R3943	T14011	T14001	prep	in,localizes
R3944	T14012	T14013	amod	mammalian,cells
R3945	T14013	T14011	pobj	cells,in
R3946	T14014	T14001	punct	", ",localizes
R3947	T14015	T14001	advcl	supporting,localizes
R3948	T14016	T14017	det	the,idea
R3949	T14017	T14015	dobj	idea,supporting
R3950	T14018	T14019	mark	that,involved
R3951	T14019	T14017	acl	involved,idea
R3952	T14020	T14021	det	the,protein
R3953	T14021	T14019	nsubjpass	protein,involved
R3954	T14022	T14023	compound	mr,s
R3955	T14023	T14021	compound	s,protein
R3956	T14024	T14023	punct	-,s
R3957	T14025	T14019	auxpass	is,involved
R3958	T14026	T14019	prep	in,involved
R3959	T14027	T14028	amod	transcriptional,regulation
R3960	T14028	T14026	pobj	regulation,in
R3961	T14029	T14030	mark	as,are
R3962	T14030	T14019	advcl	are,involved
R3963	T14031	T14030	nsubj	ph,are
R3964	T14032	T14031	cc	and,ph
R3965	T14033	T14032	punct	/,and
R3966	T14034	T14032	cc	or,and
R3967	T14035	T14031	conj	TEL,ph
R3968	T14036	T13999	punct	.,showed
R3969	T16058	T16059	det	The,protein
R3970	T16059	T16066	nsubj	protein,functions
R3971	T16060	T16061	nmod	GAL4,fusion
R3972	T16061	T16059	compound	fusion,protein
R3973	T16062	T16060	punct	-,GAL4
R3974	T16063	T16064	compound	mr,s
R3975	T16064	T16060	appos	s,GAL4
R3976	T16065	T16064	punct	-,s
R3977	T16067	T16066	prep	as,functions
R3978	T16068	T16069	amod	transcriptional,repressor
R3979	T16069	T16067	pobj	repressor,as
R3980	T16071	T16072	det	A,member
R3981	T16072	T16073	nsubj	member,contain
R3982	T16074	T16072	prep	of,member
R3983	T16075	T16076	compound	PcG,proteins
R3984	T16076	T16074	pobj	proteins,of
R3985	T16077	T16072	punct	", ",member
R3986	T16078	T16072	appos	ph,member
R3987	T16079	T16073	punct	", ",contain
R3988	T16080	T16073	aux	does,contain
R3989	T16081	T16073	neg	not,contain
R3990	T16082	T16083	det	an,motif
R3991	T16083	T16073	dobj	motif,contain
R3992	T16084	T16083	amod	obvious,motif
R3993	T16085	T16086	npadvmod	sequence,specific
R3994	T16086	T16083	amod	specific,motif
R3995	T16087	T16086	punct	-,specific
R3996	T16088	T16089	npadvmod	DNA,binding
R3997	T16089	T16083	amod	binding,motif
R3998	T16090	T16091	punct	(,16
R3999	T16091	T16073	parataxis	16,contain
R4000	T16092	T16091	punct	),16
R4001	T16093	T16073	punct	.,contain
R4002	T16095	T16096	nsubj	Ph,functions
R4003	T16097	T16096	prep	as,functions
R4004	T16098	T16099	det	a,repressor
R4005	T16099	T16097	pobj	repressor,as
R4006	T16100	T16099	amod	transcriptional,repressor
R4007	T16101	T16096	prep	through,functions
R4008	T16102	T16103	poss	its,polymerization
R4009	T16103	T16101	pobj	polymerization,through
R4010	T16104	T16103	cc	and,polymerization
R4011	T16105	T16106	compound	protein,protein
R4012	T16106	T16108	compound	protein,interaction
R4013	T16107	T16106	punct	-,protein
R4014	T16108	T16103	conj	interaction,polymerization
R4015	T16109	T16108	prep	with,interaction
R4016	T16110	T16111	amod	other,repressors
R4017	T16111	T16109	pobj	repressors,with
R4018	T16112	T16113	npadvmod	sequence,specific
R4019	T16113	T16111	amod	specific,repressors
R4020	T16114	T16113	punct	-,specific
R4021	T16115	T16111	amod	transcriptional,repressors
R4022	T16116	T16111	punct	", ",repressors
R4023	T16117	T16118	dep	which,form
R4024	T16118	T16111	relcl	form,repressors
R4025	T16119	T16118	aux	can,form
R4026	T16120	T16121	det	a,structure
R4027	T16121	T16118	dobj	structure,form
R4028	T16122	T16123	amod	higher,order
R4029	T16123	T16121	compound	order,structure
R4030	T16124	T16121	compound	chromatin,structure
R4031	T16125	T16096	punct	.,functions
R4032	T16127	T16128	det	The,protein
R4033	T16128	T16132	nsubj	protein,have
R4034	T16129	T16130	compound	mr,s
R4035	T16130	T16128	compound	s,protein
R4036	T16131	T16130	punct	-,s
R4037	T16133	T16132	advmod	also,have
R4038	T16134	T16132	aux	does,have
R4039	T16135	T16132	neg	not,have
R4040	T16136	T16137	det	an,domain
R4041	T16137	T16132	dobj	domain,have
R4042	T16138	T16137	amod	obvious,domain
R4043	T16139	T16140	npadvmod	DNA,binding
R4044	T16140	T16137	amod	binding,domain
R4045	T16141	T16140	punct	-,binding
R4046	T16142	T16132	punct	.,have
R4047	T16144	T16145	aux	To,characterize
R4048	T16145	T16146	advcl	characterize,performed
R4049	T16147	T16148	det	the,activity
R4050	T16148	T16145	dobj	activity,characterize
R4051	T16149	T16148	amod	biochemical,activity
R4052	T16150	T16148	prep	of,activity
R4053	T16151	T16152	compound	mr,s
R4054	T16152	T16150	pobj	s,of
R4055	T16153	T16152	punct	-,s
R4056	T16154	T16146	punct	", ",performed
R4057	T16155	T16146	nsubj	we,performed
R4058	T16156	T16146	advmod	next,performed
R4059	T16157	T16158	det	a,assay
R4060	T16158	T16146	dobj	assay,performed
R4061	T16159	T16158	compound	luciferase,assay
R4062	T16160	T16146	punct	.,performed
R4063	T16162	T16163	nsubj	We,generated
R4064	T16164	T16165	compound	effector,plasmids
R4065	T16165	T16163	dobj	plasmids,generated
R4066	T16166	T16165	punct	", ",plasmids
R4067	T16167	T16168	dep	which,express
R4068	T16168	T16165	relcl	express,plasmids
R4069	T16169	T16170	amod	various,constructs
R4070	T16170	T16168	dobj	constructs,express
R4071	T16171	T16170	compound	deletion,constructs
R4072	T16172	T16170	prep	of,constructs
R4073	T16173	T16174	compound	mr,s
R4074	T16174	T16172	pobj	s,of
R4075	T16175	T16174	punct	-,s
R4076	T16176	T16170	acl	fused,constructs
R4077	T16177	T16176	prep	to,fused
R4078	T16178	T16179	det	the,domain
R4079	T16179	T16177	pobj	domain,to
R4080	T16180	T16179	nmod	GAL4,domain
R4081	T16181	T16182	npadvmod	DNA,binding
R4082	T16182	T16179	amod	binding,domain
R4083	T16183	T16182	punct	-,binding
R4084	T16184	T16185	punct	(,7A
R4085	T16185	T16163	parataxis	7A,generated
R4086	T16186	T16185	compound	Fig.,7A
R4087	T16187	T16185	punct	),7A
R4088	T16188	T16163	punct	.,generated
R4089	T16190	T16191	nsubj	We,confirmed
R4090	T16192	T16191	advmod	first,confirmed
R4091	T16193	T16194	mark	that,had
R4092	T16194	T16191	ccomp	had,confirmed
R4093	T16195	T16196	amod	full,length
R4094	T16196	T16198	compound	length,s
R4095	T16197	T16196	punct	-,length
R4096	T16198	T16194	nsubj	s,had
R4097	T16199	T16198	compound	mr,s
R4098	T16200	T16198	punct	-,s
R4099	T16201	T16198	acl	fused,s
R4100	T16202	T16201	prep	to,fused
R4101	T16203	T16204	compound	GAL4,domain
R4102	T16204	T16202	pobj	domain,to
R4103	T16205	T16206	compound	DNA,binding
R4104	T16206	T16204	compound	binding,domain
R4105	T16207	T16204	punct	(,domain
R4106	T16208	T16209	compound	DBD,mrs
R4107	T16209	T16204	appos	mrs,domain
R4108	T16210	T16209	punct	-,mrs
R4109	T16211	T16194	punct	),had
R4110	T16212	T16213	det	no,effect
R4111	T16213	T16194	dobj	effect,had
R4112	T16214	T16213	prep	on,effect
R4113	T16215	T16216	det	the,plasmid
R4114	T16216	T16214	pobj	plasmid,on
R4115	T16217	T16216	compound	pGL3,plasmid
R4116	T16218	T16216	compound	promoter,plasmid
R4117	T16219	T16216	acl	lacking,plasmid
R4118	T16220	T16221	compound	GAL4,binding
R4119	T16221	T16222	compound	binding,sites
R4120	T16222	T16219	dobj	sites,lacking
R4121	T16223	T16224	punct	(,shown
R4122	T16224	T16191	parataxis	shown,confirmed
R4123	T16225	T16224	nsubj	data,shown
R4124	T16226	T16224	neg	not,shown
R4125	T16227	T16224	punct	),shown
R4126	T16228	T16191	punct	.,confirmed
R4127	T16230	T16231	advmod	When,co-transfected
R4128	T16231	T16239	advcl	co-transfected,repressed
R4129	T16232	T16233	det	the,plasmid
R4130	T16233	T16231	nsubjpass	plasmid,co-transfected
R4131	T16234	T16235	compound	5xGAL4,pGL3
R4132	T16235	T16233	compound	pGL3,plasmid
R4133	T16236	T16235	punct	-,pGL3
R4134	T16237	T16233	compound	reporter,plasmid
R4135	T16238	T16231	auxpass	was,co-transfected
R4136	T16240	T16239	punct	", ",repressed
R4137	T16241	T16242	compound	DBD,mrs
R4138	T16242	T16239	nsubj	mrs,repressed
R4139	T16243	T16242	punct	-,mrs
R4140	T16244	T16245	compound	luciferase,activity
R4141	T16245	T16239	dobj	activity,repressed
R4142	T16246	T16239	prep	by,repressed
R4143	T16247	T16248	quantmod	about,90
R4144	T16248	T16249	nummod	90,%
R4145	T16249	T16246	pobj	%,by
R4146	T16250	T16239	prep	in,repressed
R4147	T16251	T16252	det	a,manner
R4148	T16252	T16250	pobj	manner,in
R4149	T16253	T16254	npadvmod	dose,dependent
R4150	T16254	T16252	amod	dependent,manner
R4151	T16255	T16254	punct	-,dependent
R4152	T16256	T16257	punct	(,7B
R4153	T16257	T16239	parataxis	7B,repressed
R4154	T16258	T16257	compound	Fig.,7B
R4155	T16259	T16257	punct	),7B
R4156	T16260	T16239	punct	.,repressed
R4157	T16262	T16263	prep	As,had
R4158	T16264	T16265	det	a,control
R4159	T16265	T16262	pobj	control,As
R4160	T16266	T16263	punct	", ",had
R4161	T16267	T16268	nmod	GAL4,domain
R4162	T16268	T16263	nsubj	domain,had
R4163	T16269	T16270	npadvmod	DNA,binding
R4164	T16270	T16268	amod	binding,domain
R4165	T16271	T16270	punct	-,binding
R4166	T16272	T16268	punct	(,domain
R4167	T16273	T16268	appos	DBD,domain
R4168	T16274	T16263	punct	),had
R4169	T16275	T16276	det	no,effect
R4170	T16276	T16263	dobj	effect,had
R4171	T16277	T16276	amod	significant,effect
R4172	T16278	T16276	prep	on,effect
R4173	T16279	T16280	det	the,plasmid
R4174	T16280	T16278	pobj	plasmid,on
R4175	T16281	T16282	compound	5xGAL4,pGL3
R4176	T16282	T16280	compound	pGL3,plasmid
R4177	T16283	T16282	punct	-,pGL3
R4178	T16284	T16280	compound	reporter,plasmid
R4179	T16285	T16263	punct	.,had
R4180	T16287	T16288	prep	In,confirmed
R4181	T16289	T16287	pobj	addition,In
R4182	T16290	T16288	punct	", ",confirmed
R4183	T16291	T16288	nsubj	we,confirmed
R4184	T16292	T16293	mark	that,had
R4185	T16293	T16288	ccomp	had,confirmed
R4186	T16294	T16295	amod	full,length
R4187	T16295	T16297	compound	length,s
R4188	T16296	T16295	punct	-,length
R4189	T16297	T16293	nsubj	s,had
R4190	T16298	T16297	compound	mr,s
R4191	T16299	T16297	punct	-,s
R4192	T16300	T16297	prep	without,s
R4193	T16301	T16302	compound	GAL4,DBD
R4194	T16302	T16300	pobj	DBD,without
R4195	T16303	T16304	det	no,effect
R4196	T16304	T16293	dobj	effect,had
R4197	T16305	T16304	prep	on,effect
R4198	T16306	T16307	det	the,plasmid
R4199	T16307	T16305	pobj	plasmid,on
R4200	T16308	T16307	amod	same,plasmid
R4201	T16309	T16307	compound	reporter,plasmid
R4202	T16310	T16311	punct	(,shown
R4203	T16311	T16288	parataxis	shown,confirmed
R4204	T16312	T16311	nsubj	data,shown
R4205	T16313	T16311	neg	not,shown
R4206	T16314	T16311	punct	),shown
R4207	T16315	T16288	punct	.,confirmed
R4208	T16317	T16318	nsubj	We,analyzed
R4209	T16319	T16318	advmod	next,analyzed
R4210	T16320	T16321	compound	deletion,constructs
R4211	T16321	T16318	dobj	constructs,analyzed
R4212	T16322	T16323	prep	in,fused
R4213	T16323	T16321	relcl	fused,constructs
R4214	T16324	T16322	pobj	which,in
R4215	T16325	T16326	det	the,stretch
R4216	T16326	T16323	nsubjpass	stretch,fused
R4217	T16327	T16328	nmod	N,terminus
R4218	T16328	T16326	nmod	terminus,stretch
R4219	T16329	T16328	punct	-,terminus
R4220	T16330	T16331	nummod	400,aa
R4221	T16331	T16326	compound	aa,stretch
R4222	T16332	T16326	prep	of,stretch
R4223	T16333	T16334	compound	mr,s
R4224	T16334	T16332	pobj	s,of
R4225	T16335	T16334	punct	-,s
R4226	T16336	T16337	punct	(,1
R4227	T16337	T16326	parataxis	1,stretch
R4228	T16338	T16337	nmod	amino,1
R4229	T16339	T16337	nmod	acids,1
R4230	T16340	T16337	prep	to,1
R4231	T16341	T16340	pobj	400,to
R4232	T16342	T16337	punct	),1
R4233	T16343	T16326	cc	or,stretch
R4234	T16344	T16345	det	the,portion
R4235	T16345	T16326	conj	portion,stretch
R4236	T16346	T16347	compound	C,terminus
R4237	T16347	T16345	compound	terminus,portion
R4238	T16348	T16347	punct	-,terminus
R4239	T16349	T16350	punct	(,391
R4240	T16350	T16345	parataxis	391,portion
R4241	T16351	T16350	nmod	amino,391
R4242	T16352	T16350	nmod	acids,391
R4243	T16353	T16350	prep	to,391
R4244	T16354	T16353	pobj	542,to
R4245	T16355	T16350	punct	),391
R4246	T16356	T16323	auxpass	were,fused
R4247	T16357	T16323	prep	to,fused
R4248	T16358	T16359	det	the,DBD
R4249	T16359	T16357	pobj	DBD,to
R4250	T16360	T16359	compound	GAL4,DBD
R4251	T16361	T16362	punct	(,7A
R4252	T16362	T16323	parataxis	7A,fused
R4253	T16363	T16362	compound	Fig.,7A
R4254	T16364	T16362	punct	", ",7A
R4255	T16365	T16366	compound	DBD,N
R4256	T16366	T16362	appos	N,7A
R4257	T16367	T16366	punct	-,N
R4258	T16368	T16362	punct	", ",7A
R4259	T16369	T16370	compound	DBD,C
R4260	T16370	T16362	appos	C,7A
R4261	T16371	T16370	punct	-,C
R4262	T16372	T16362	punct	),7A
R4263	T16373	T16318	punct	.,analyzed
R4264	T16375	T16376	mark	While,had
R4265	T16376	T16380	advcl	had,repressed
R4266	T16377	T16378	compound	DBD,N
R4267	T16378	T16376	nsubj	N,had
R4268	T16379	T16378	punct	-,N
R4269	T16381	T16382	det	no,effect
R4270	T16382	T16376	dobj	effect,had
R4271	T16383	T16382	amod	repressive,effect
R4272	T16384	T16382	prep	on,effect
R4273	T16385	T16386	det	this,activity
R4274	T16386	T16384	pobj	activity,on
R4275	T16387	T16386	compound	reporter,activity
R4276	T16388	T16380	punct	", ",repressed
R4277	T16389	T16390	compound	DBD,C
R4278	T16390	T16380	nsubj	C,repressed
R4279	T16391	T16390	punct	-,C
R4280	T16392	T16393	compound	luciferase,expression
R4281	T16393	T16380	dobj	expression,repressed
R4282	T16394	T16393	compound	gene,expression
R4283	T16395	T16380	prep	by,repressed
R4284	T16396	T16397	quantmod	about,65
R4285	T16397	T16398	nummod	65,%
R4286	T16398	T16395	pobj	%,by
R4287	T16399	T16380	punct	.,repressed
R4288	T16401	T16402	det	This,result
R4289	T16402	T16403	nsubj	result,suggested
R4290	T16404	T16403	prep	to,suggested
R4291	T16405	T16404	pobj	us,to
R4292	T16406	T16407	det	the,possibility
R4293	T16407	T16403	dobj	possibility,suggested
R4294	T16408	T16409	mark	that,exerts
R4295	T16409	T16407	acl	exerts,possibility
R4296	T16410	T16411	compound	DBD,mrs
R4297	T16411	T16409	nsubj	mrs,exerts
R4298	T16412	T16411	punct	-,mrs
R4299	T16413	T16414	amod	transcriptional,activity
R4300	T16414	T16409	dobj	activity,exerts
R4301	T16415	T16414	amod	repressive,activity
R4302	T16416	T16409	prep	via,exerts
R4303	T16417	T16418	compound	self,association
R4304	T16418	T16416	pobj	association,via
R4305	T16419	T16418	punct	-,association
R4306	T16420	T16409	prep	through,exerts
R4307	T16421	T16422	poss	its,domain
R4308	T16422	T16420	pobj	domain,through
R4309	T16423	T16422	compound	SAM,domain
R4310	T16424	T16403	punct	.,suggested
R4311	T16426	T16427	aux	To,investigate
R4312	T16427	T16428	advcl	investigate,analyzed
R4313	T16429	T16430	mark	whether,required
R4314	T16430	T16427	ccomp	required,investigate
R4315	T16431	T16432	det	the,association
R4316	T16432	T16430	nsubjpass	association,required
R4317	T16433	T16432	amod	homophilic,association
R4318	T16434	T16432	prep	of,association
R4319	T16435	T16436	compound	mr,s
R4320	T16436	T16434	pobj	s,of
R4321	T16437	T16436	punct	-,s
R4322	T16438	T16430	auxpass	is,required
R4323	T16439	T16430	prep	for,required
R4324	T16440	T16441	amod	transcriptional,repression
R4325	T16441	T16439	pobj	repression,for
R4326	T16442	T16428	punct	", ",analyzed
R4327	T16443	T16444	nummod	two,mutants
R4328	T16444	T16428	nsubjpass	mutants,analyzed
R4329	T16445	T16446	npadvmod	site,directed
R4330	T16446	T16444	amod	directed,mutants
R4331	T16447	T16446	punct	-,directed
R4332	T16448	T16444	punct	", ",mutants
R4333	T16449	T16450	compound	DBD,W404A
R4334	T16450	T16444	appos	W404A,mutants
R4335	T16451	T16450	punct	-,W404A
R4336	T16452	T16450	cc	and,W404A
R4337	T16453	T16454	compound	DBD,G453A
R4338	T16454	T16450	conj	G453A,W404A
R4339	T16455	T16454	punct	-,G453A
R4340	T16456	T16444	punct	", ",mutants
R4341	T16457	T16458	dep	either,reduce
R4342	T16458	T16444	relcl	reduce,mutants
R4343	T16459	T16457	prep	of,either
R4344	T16460	T16459	pobj	which,of
R4345	T16461	T16458	aux	may,reduce
R4346	T16462	T16463	npadvmod	self,binding
R4347	T16463	T16465	amod	binding,ability
R4348	T16464	T16463	punct	-,binding
R4349	T16465	T16458	dobj	ability,reduce
R4350	T16466	T16428	punct	", ",analyzed
R4351	T16467	T16428	auxpass	were,analyzed
R4352	T16468	T16469	punct	(,7C
R4353	T16469	T16428	parataxis	7C,analyzed
R4354	T16470	T16469	compound	Fig.,7C
R4355	T16471	T16469	punct	),7C
R4356	T16472	T16428	punct	.,analyzed
R4357	T16474	T16475	prep	Compared,had
R4358	T16476	T16474	prep	to,Compared
R4359	T16477	T16478	compound	DBD,mrs
R4360	T16478	T16476	pobj	mrs,to
R4361	T16479	T16478	punct	-,mrs
R4362	T16480	T16475	punct	", ",had
R4363	T16481	T16482	compound	DBD,W404A
R4364	T16482	T16475	nsubj	W404A,had
R4365	T16483	T16482	punct	-,W404A
R4366	T16484	T16482	cc	and,W404A
R4367	T16485	T16486	compound	DBD,G453A
R4368	T16486	T16482	conj	G453A,W404A
R4369	T16487	T16486	punct	-,G453A
R4370	T16488	T16489	compound	repression,activity
R4371	T16489	T16475	dobj	activity,had
R4372	T16490	T16489	prep	of,activity
R4373	T16491	T16492	nummod	72,%
R4374	T16492	T16490	pobj	%,of
R4375	T16493	T16492	cc	and,%
R4376	T16494	T16495	nummod	87,%
R4377	T16495	T16492	conj	%,%
R4378	T16496	T16475	punct	", ",had
R4379	T16497	T16475	advmod	respectively,had
R4380	T16498	T16475	punct	.,had
R4381	T16500	T16501	mark	While,correlates
R4382	T16501	T16512	advcl	correlates,compromise
R4383	T16502	T16503	det	the,ability
R4384	T16503	T16501	nsubj	ability,correlates
R4385	T16504	T16503	prep	of,ability
R4386	T16505	T16506	compound	mr,s
R4387	T16506	T16508	compound	s,association
R4388	T16507	T16506	punct	-,s
R4389	T16508	T16504	pobj	association,of
R4390	T16509	T16508	compound	self,association
R4391	T16510	T16508	punct	-,association
R4392	T16511	T16501	advmod	partially,correlates
R4393	T16513	T16501	prep	with,correlates
R4394	T16514	T16515	amod	transcriptional,activity
R4395	T16515	T16513	pobj	activity,with
R4396	T16516	T16515	amod	repressive,activity
R4397	T16517	T16512	punct	", ",compromise
R4398	T16518	T16519	det	these,mutants
R4399	T16519	T16512	nsubj	mutants,compromise
R4400	T16520	T16512	aux	do,compromise
R4401	T16521	T16512	neg	not,compromise
R4402	T16522	T16523	det	the,ability
R4403	T16523	T16512	dobj	ability,compromise
R4404	T16524	T16525	aux	to,repress
R4405	T16525	T16523	acl	repress,ability
R4406	T16526	T16525	dobj	transcription,repress
R4407	T16527	T16525	advmod	critically,repress
R4408	T16528	T16512	punct	.,compromise
R4409	T16530	T16531	aux	To,determine
R4410	T16531	T16532	advcl	determine,divided
R4411	T16533	T16534	det	the,regions
R4412	T16534	T16531	dobj	regions,determine
R4413	T16535	T16534	prep	of,regions
R4414	T16536	T16537	compound	mr,s
R4415	T16537	T16535	pobj	s,of
R4416	T16538	T16537	punct	-,s
R4417	T16539	T16534	acl	involved,regions
R4418	T16540	T16539	prep	in,involved
R4419	T16541	T16542	amod	transcriptional,repression
R4420	T16542	T16540	pobj	repression,in
R4421	T16543	T16544	advmod	more,precisely
R4422	T16544	T16531	advmod	precisely,determine
R4423	T16545	T16532	punct	", ",divided
R4424	T16546	T16547	det	the,portion
R4425	T16547	T16532	nsubjpass	portion,divided
R4426	T16548	T16549	compound	C,terminus
R4427	T16549	T16547	compound	terminus,portion
R4428	T16550	T16549	punct	-,terminus
R4429	T16551	T16532	auxpass	was,divided
R4430	T16552	T16532	prep	into,divided
R4431	T16553	T16554	nummod	two,regions
R4432	T16554	T16552	pobj	regions,into
R4433	T16555	T16532	cc	and,divided
R4434	T16556	T16557	nsubjpass	each,fused
R4435	T16557	T16532	conj	fused,divided
R4436	T16558	T16557	auxpass	was,fused
R4437	T16559	T16557	prep	to,fused
R4438	T16560	T16561	det	the,DBD
R4439	T16561	T16559	pobj	DBD,to
R4440	T16562	T16561	compound	GAL4,DBD
R4441	T16563	T16564	punct	(,SAM
R4442	T16564	T16557	parataxis	SAM,fused
R4443	T16565	T16566	compound	Fig.,7A
R4444	T16566	T16564	dep	7A,SAM
R4445	T16567	T16564	punct	", ",SAM
R4446	T16568	T16569	compound	DBD,tail
R4447	T16569	T16564	dep	tail,SAM
R4448	T16570	T16569	punct	-,tail
R4449	T16571	T16564	punct	", ",SAM
R4450	T16572	T16564	compound	DBD,SAM
R4451	T16573	T16564	punct	-,SAM
R4452	T16574	T16564	punct	),SAM
R4453	T16575	T16532	punct	.,divided
R4454	T16577	T16578	prep	As,have
R4455	T16579	T16580	det	a,consequence
R4456	T16580	T16577	pobj	consequence,As
R4457	T16581	T16578	punct	", ",have
R4458	T16582	T16583	compound	DBD,SAM
R4459	T16583	T16578	nsubj	SAM,have
R4460	T16584	T16583	punct	-,SAM
R4461	T16585	T16586	punct	(,384
R4462	T16586	T16583	parataxis	384,SAM
R4463	T16587	T16586	nmod	amino,384
R4464	T16588	T16586	nmod	acids,384
R4465	T16589	T16586	prep	to,384
R4466	T16590	T16589	pobj	462,to
R4467	T16591	T16586	punct	),384
R4468	T16592	T16578	aux	did,have
R4469	T16593	T16578	neg	not,have
R4470	T16594	T16595	det	a,effect
R4471	T16595	T16578	dobj	effect,have
R4472	T16596	T16595	amod	repressive,effect
R4473	T16597	T16595	prep	on,effect
R4474	T16598	T16599	det	the,plasmid
R4475	T16599	T16597	pobj	plasmid,on
R4476	T16600	T16601	compound	5xGAL4,pGL3
R4477	T16601	T16599	compound	pGL3,plasmid
R4478	T16602	T16601	punct	-,pGL3
R4479	T16603	T16599	compound	reporter,plasmid
R4480	T16604	T16578	punct	.,have
R4481	T16606	T16607	prep	On,repressed
R4482	T16608	T16609	det	the,hand
R4483	T16609	T16606	pobj	hand,On
R4484	T16610	T16609	amod	other,hand
R4485	T16611	T16607	punct	", ",repressed
R4486	T16612	T16613	compound	luciferase,activity
R4487	T16613	T16607	nsubjpass	activity,repressed
R4488	T16614	T16607	auxpass	was,repressed
R4489	T16615	T16607	prep	by,repressed
R4490	T16616	T16617	nummod	55,%
R4491	T16617	T16615	pobj	%,by
R4492	T16618	T16619	advmod	when,co-transfected
R4493	T16619	T16607	advcl	co-transfected,repressed
R4494	T16620	T16621	compound	DBD,tail
R4495	T16621	T16619	nsubjpass	tail,co-transfected
R4496	T16622	T16621	punct	-,tail
R4497	T16623	T16624	punct	(,459
R4498	T16624	T16621	parataxis	459,tail
R4499	T16625	T16624	nmod	amino,459
R4500	T16626	T16624	nmod	acids,459
R4501	T16627	T16624	prep	to,459
R4502	T16628	T16627	pobj	542,to
R4503	T16629	T16624	punct	),459
R4504	T16630	T16619	auxpass	was,co-transfected
R4505	T16631	T16619	prep	with,co-transfected
R4506	T16632	T16633	det	this,plasmid
R4507	T16633	T16631	pobj	plasmid,with
R4508	T16634	T16633	compound	reporter,plasmid
R4509	T16635	T16636	punct	(,7D
R4510	T16636	T16619	parataxis	7D,co-transfected
R4511	T16637	T16636	compound	Fig.,7D
R4512	T16638	T16636	punct	),7D
R4513	T16639	T16607	punct	.,repressed
R4514	T16641	T16642	aux	To,assess
R4515	T16642	T16643	advcl	assess,performed
R4516	T16644	T16645	det	the,activity
R4517	T16645	T16642	dobj	activity,assess
R4518	T16646	T16645	amod	transcriptional,activity
R4519	T16647	T16645	amod	repressive,activity
R4520	T16648	T16645	prep	of,activity
R4521	T16649	T16650	compound	mr,s
R4522	T16650	T16648	pobj	s,of
R4523	T16651	T16650	punct	-,s
R4524	T16652	T16642	prep	in,assess
R4525	T16653	T16652	pobj	cells,in
R4526	T16654	T16653	prep	of,cells
R4527	T16655	T16656	amod	retinal,origin
R4528	T16656	T16654	pobj	origin,of
R4529	T16657	T16643	punct	", ",performed
R4530	T16658	T16643	nsubj	we,performed
R4531	T16659	T16660	amod	similar,experiments
R4532	T16660	T16643	dobj	experiments,performed
R4533	T16661	T16643	advcl	using,performed
R4534	T16662	T16663	amod	human,cells
R4535	T16663	T16661	dobj	cells,using
R4536	T16664	T16663	compound	Y79,cells
R4537	T16665	T16663	compound	retinoblastoma,cells
R4538	T16666	T16643	punct	.,performed
R4539	T16668	T16669	det	The,results
R4540	T16669	T16670	nsubj	results,indicated
R4541	T16671	T16672	mark	that,reduced
R4542	T16672	T16670	ccomp	reduced,indicated
R4543	T16673	T16674	compound	DBD,mrs
R4544	T16674	T16672	nsubj	mrs,reduced
R4545	T16675	T16674	punct	-,mrs
R4546	T16676	T16672	advmod	also,reduced
R4547	T16677	T16678	compound	luciferase,activity
R4548	T16678	T16672	dobj	activity,reduced
R4549	T16679	T16672	advmod	significantly,reduced
R4550	T16680	T16672	prep	in,reduced
R4551	T16681	T16682	compound	Y79,cells
R4552	T16682	T16680	pobj	cells,in
R4553	T16683	T16682	compound	retinoblastoma,cells
R4554	T16684	T16685	punct	(,7E
R4555	T16685	T16672	parataxis	7E,reduced
R4556	T16686	T16685	compound	Fig.,7E
R4557	T16687	T16685	punct	),7E
R4558	T16688	T16670	punct	.,indicated
R4559	T16690	T16691	advmod	However,repressed
R4560	T16692	T16691	punct	", ",repressed
R4561	T16693	T16694	compound	luciferase,activity
R4562	T16694	T16691	nsubjpass	activity,repressed
R4563	T16695	T16691	auxpass	was,repressed
R4564	T16696	T16691	prep	by,repressed
R4565	T16697	T16698	quantmod	about,30
R4566	T16698	T16699	nummod	30,%
R4567	T16699	T16696	pobj	%,by
R4568	T16700	T16691	prep	in,repressed
R4569	T16701	T16702	compound	Y79,cells
R4570	T16702	T16700	pobj	cells,in
R4571	T16703	T16691	punct	", ",repressed
R4572	T16704	T16705	mark	while,repressed
R4573	T16705	T16691	advcl	repressed,repressed
R4574	T16706	T16705	nsubjpass	it,repressed
R4575	T16707	T16705	auxpass	was,repressed
R4576	T16708	T16705	prep	by,repressed
R4577	T16709	T16710	nummod	90,%
R4578	T16710	T16708	pobj	%,by
R4579	T16711	T16705	prep	in,repressed
R4580	T16712	T16713	compound	HEK293T,cells
R4581	T16713	T16711	pobj	cells,in
R4582	T16714	T16691	punct	.,repressed
R4583	T16716	T16717	nsubj	This,be
R4584	T16718	T16717	aux	might,be
R4585	T16719	T16717	prep	due,be
R4586	T16720	T16719	pcomp	to,due
R4587	T16721	T16722	det	the,difference
R4588	T16722	T16719	pobj	difference,due
R4589	T16723	T16722	prep	in,difference
R4590	T16724	T16725	compound	transfection,efficiency
R4591	T16725	T16723	pobj	efficiency,in
R4592	T16726	T16722	prep	between,difference
R4593	T16727	T16728	det	these,lines
R4594	T16728	T16726	pobj	lines,between
R4595	T16729	T16728	compound	cell,lines
R4596	T16730	T16717	punct	.,be
R4597	T16732	T16733	det	Another,possibility
R4598	T16733	T16734	nsubj	possibility,is
R4599	T16735	T16736	mark	that,is
R4600	T16736	T16734	ccomp	is,is
R4601	T16737	T16738	amod	intracellular,environment
R4602	T16738	T16736	nsubj	environment,is
R4603	T16739	T16738	prep	in,environment
R4604	T16740	T16741	compound	Y79,cells
R4605	T16741	T16739	pobj	cells,in
R4606	T16742	T16741	punct	", ",cells
R4607	T16743	T16744	det	a,line
R4608	T16744	T16741	appos	line,cells
R4609	T16745	T16744	compound	retinoblastoma,line
R4610	T16746	T16744	compound	cell,line
R4611	T16747	T16736	punct	", ",is
R4612	T16748	T16736	acomp	insufficient,is
R4613	T16749	T16748	prep	for,insufficient
R4614	T16750	T16749	pcomp	recapitulating,for
R4615	T16751	T16752	amod	developing,photoreceptors
R4616	T16752	T16750	dobj	photoreceptors,recapitulating
R4617	T16753	T16734	punct	.,is
R4618	T16755	T16756	prep	In,address
R4619	T16757	T16758	det	this,study
R4620	T16758	T16755	pobj	study,In
R4621	T16759	T16756	punct	", ",address
R4622	T16760	T16756	nsubj	we,address
R4623	T16761	T16756	aux	did,address
R4624	T16762	T16756	neg	not,address
R4625	T16763	T16764	det	the,question
R4626	T16764	T16756	dobj	question,address
R4627	T16765	T16766	mark	whether,reflect
R4628	T16766	T16764	acl	reflect,question
R4629	T16767	T16766	cc	or,reflect
R4630	T16768	T16766	neg	not,reflect
R4631	T16769	T16770	poss	our,data
R4632	T16770	T16766	nsubj	data,reflect
R4633	T16771	T16772	advmod	in,vitro
R4634	T16772	T16770	amod	vitro,data
R4635	T16773	T16774	amod	native,repression
R4636	T16774	T16766	dobj	repression,reflect
R4637	T16775	T16776	nmod	mr,s
R4638	T16776	T16774	nmod	s,repression
R4639	T16777	T16776	punct	-,s
R4640	T16778	T16774	amod	transcriptional,repression
R4641	T16779	T16780	advmod	in,vivo
R4642	T16780	T16766	advmod	vivo,reflect
R4643	T16781	T16756	punct	.,address
R4644	T16783	T16784	advmod	However,support
R4645	T16785	T16784	punct	", ",support
R4646	T16786	T16787	det	these,experiments
R4647	T16787	T16784	nsubj	experiments,support
R4648	T16788	T16789	advmod	in,vitro
R4649	T16789	T16787	amod	vitro,experiments
R4650	T16790	T16787	acl	using,experiments
R4651	T16791	T16792	nmod	HEK293T,cells
R4652	T16792	T16790	dobj	cells,using
R4653	T16793	T16791	cc	and,HEK293T
R4654	T16794	T16791	conj	Y79,HEK293T
R4655	T16795	T16784	advmod	strongly,support
R4656	T16796	T16797	poss	our,hypothesis
R4657	T16797	T16784	dobj	hypothesis,support
R4658	T16798	T16799	mark	that,functions
R4659	T16799	T16797	acl	functions,hypothesis
R4660	T16800	T16801	compound	mr,s
R4661	T16801	T16799	nsubj	s,functions
R4662	T16802	T16801	punct	-,s
R4663	T16803	T16799	prep	as,functions
R4664	T16804	T16805	det	a,repressor
R4665	T16805	T16803	pobj	repressor,as
R4666	T16806	T16805	amod	transcriptional,repressor
R4667	T16807	T16799	prep	in,functions
R4668	T16808	T16809	amod	developing,photoreceptors
R4669	T16809	T16807	pobj	photoreceptors,in
R4670	T16810	T16784	punct	.,support
R4671	T16812	T16813	poss	Our,results
R4672	T16813	T16814	nsubj	results,suggested
R4673	T16815	T16816	mark	that,required
R4674	T16816	T16814	ccomp	required,suggested
R4675	T16817	T16818	det	the,region
R4676	T16818	T16816	nsubjpass	region,required
R4677	T16819	T16820	npadvmod	C,terminal
R4678	T16820	T16818	amod	terminal,region
R4679	T16821	T16820	punct	-,terminal
R4680	T16822	T16818	prep	of,region
R4681	T16823	T16824	compound	mr,s
R4682	T16824	T16822	pobj	s,of
R4683	T16825	T16824	punct	-,s
R4684	T16826	T16827	punct	(,463
R4685	T16827	T16818	parataxis	463,region
R4686	T16828	T16827	nmod	amino,463
R4687	T16829	T16827	nmod	acids,463
R4688	T16830	T16827	prep	to,463
R4689	T16831	T16830	pobj	542,to
R4690	T16832	T16827	punct	),463
R4691	T16833	T16816	auxpass	is,required
R4692	T16834	T16816	prep	for,required
R4693	T16835	T16836	amod	transcriptional,repression
R4694	T16836	T16834	pobj	repression,for
R4695	T16837	T16836	prep	of,repression
R4696	T16838	T16839	compound	mr,s
R4697	T16839	T16837	pobj	s,of
R4698	T16840	T16839	punct	-,s
R4699	T16841	T16816	cc	and,required
R4700	T16842	T16843	det	the,domain
R4701	T16843	T16845	nsubj	domain,appears
R4702	T16844	T16843	compound	SAM,domain
R4703	T16845	T16816	conj	appears,required
R4704	T16846	T16847	aux	to,be
R4705	T16847	T16845	xcomp	be,appears
R4706	T16848	T16847	acomp	dispensable,be
R4707	T16849	T16848	prep	for,dispensable
R4708	T16850	T16851	det	this,activity
R4709	T16851	T16849	pobj	activity,for
R4710	T16852	T16851	amod	repressive,activity
R4711	T16853	T16814	punct	.,suggested
R4712	T16855	T16856	det	This,region
R4713	T16856	T16860	nsubjpass	region,conserved
R4714	T16857	T16858	npadvmod	C,terminal
R4715	T16858	T16856	amod	terminal,region
R4716	T16859	T16858	punct	-,terminal
R4717	T16861	T16856	prep	of,region
R4718	T16862	T16863	compound	mouse,s
R4719	T16863	T16861	pobj	s,of
R4720	T16864	T16863	compound	mr,s
R4721	T16865	T16863	punct	-,s
R4722	T16866	T16860	auxpass	is,conserved
R4723	T16867	T16860	advmod	highly,conserved
R4724	T16868	T16860	prep	among,conserved
R4725	T16869	T16868	pobj	species,among
R4726	T16870	T16871	punct	(,7F
R4727	T16871	T16860	parataxis	7F,conserved
R4728	T16872	T16871	compound	Fig.,7F
R4729	T16873	T16871	punct	),7F
R4730	T16874	T16860	punct	.,conserved
R4731	T16876	T16877	det	The,identity
R4732	T16877	T16879	nsubj	identity,was
R4733	T16878	T16877	compound	sequence,identity
R4734	T16880	T16877	prep	of,identity
R4735	T16881	T16882	det	the,region
R4736	T16882	T16880	pobj	region,of
R4737	T16883	T16884	nummod	93,%
R4738	T16884	T16879	attr	%,was
R4739	T16885	T16884	punct	", ",%
R4740	T16886	T16887	nummod	73,%
R4741	T16887	T16884	conj	%,%
R4742	T16888	T16887	punct	", ",%
R4743	T16889	T16890	nummod	41,%
R4744	T16890	T16887	conj	%,%
R4745	T16891	T16890	cc	and,%
R4746	T16892	T16893	nummod	40,%
R4747	T16893	T16890	conj	%,%
R4748	T16894	T16884	prep	for,%
R4749	T16895	T16894	pobj	rat,for
R4750	T16896	T16895	punct	", ",rat
R4751	T16897	T16895	amod	human,rat
R4752	T16898	T16895	punct	", ",rat
R4753	T16899	T16895	conj	chick,rat
R4754	T16900	T16899	cc	and,chick
R4755	T16901	T16899	conj	zebrafish,chick
R4756	T16902	T16884	punct	", ",%
R4757	T16903	T16884	advmod	respectively,%
R4758	T16904	T16905	punct	(,1D
R4759	T16905	T16879	parataxis	1D,was
R4760	T16906	T16905	compound	Fig.,1D
R4761	T16907	T16905	punct	),1D
R4762	T16908	T16879	punct	.,was
R4763	T16910	T16911	nsubj	This,suggests
R4764	T16912	T16911	advmod	strongly,suggests
R4765	T16913	T16914	mark	that,functions
R4766	T16914	T16911	ccomp	functions,suggests
R4767	T16915	T16916	det	the,region
R4768	T16916	T16914	nsubj	region,functions
R4769	T16917	T16918	npadvmod	C,terminal
R4770	T16918	T16916	amod	terminal,region
R4771	T16919	T16918	punct	-,terminal
R4772	T16920	T16916	prep	of,region
R4773	T16921	T16922	compound	mouse,s
R4774	T16922	T16920	pobj	s,of
R4775	T16923	T16922	compound	mr,s
R4776	T16924	T16922	punct	-,s
R4777	T16925	T16914	prep	as,functions
R4778	T16926	T16927	det	a,domain
R4779	T16927	T16925	pobj	domain,as
R4780	T16928	T16927	amod	transcriptional,domain
R4781	T16929	T16927	amod	repressive,domain
R4782	T16930	T16927	prep	in,domain
R4783	T16931	T16932	compound	photoreceptor,development
R4784	T16932	T16930	pobj	development,in
R4785	T16933	T16911	punct	.,suggests
R4786	T16935	T16936	advmod	However,contain
R4787	T16937	T16936	punct	", ",contain
R4788	T16938	T16939	det	this,region
R4789	T16939	T16936	nsubj	region,contain
R4790	T16940	T16936	aux	does,contain
R4791	T16941	T16936	neg	not,contain
R4792	T16942	T16936	amod	characteristic,contain
R4793	T16943	T16944	compound	amino,acid
R4794	T16944	T16936	dobj	acid,contain
R4795	T16945	T16936	dep	motifs,contain
R4796	T16946	T16936	cc	and,contain
R4797	T16947	T16948	det	the,mechanism
R4798	T16948	T16949	nsubj	mechanism,remains
R4799	T16949	T16936	conj	remains,contain
R4800	T16950	T16951	prep	through,achieves
R4801	T16951	T16948	relcl	achieves,mechanism
R4802	T16952	T16950	pobj	which,through
R4803	T16953	T16954	det	the,region
R4804	T16954	T16951	nsubj	region,achieves
R4805	T16955	T16951	cc	and,achieves
R4806	T16956	T16955	punct	/,and
R4807	T16957	T16955	cc	or,and
R4808	T16958	T16951	conj	maintains,achieves
R4809	T16959	T16960	compound	gene,repression
R4810	T16960	T16958	dobj	repression,maintains
R4811	T16961	T16962	aux	to,clarified
R4812	T16962	T16949	xcomp	clarified,remains
R4813	T16963	T16962	auxpass	be,clarified
R4814	T16964	T16962	prep	in,clarified
R4815	T16965	T16966	det	the,future
R4816	T16966	T16964	pobj	future,in
R4817	T16967	T16936	punct	.,contain
R4818	T16969	T16970	advcl	Taken,suggest
R4819	T16971	T16969	advmod	together,Taken
R4820	T16972	T16970	punct	", ",suggest
R4821	T16973	T16974	poss	our,findings
R4822	T16974	T16970	nsubj	findings,suggest
R4823	T16975	T16976	mark	that,functions
R4824	T16976	T16970	advcl	functions,suggest
R4825	T16977	T16978	compound	DBD,mrs
R4826	T16978	T16976	nsubj	mrs,functions
R4827	T16979	T16978	punct	-,mrs
R4828	T16980	T16976	prep	as,functions
R4829	T16981	T16982	det	a,repressor
R4830	T16982	T16980	pobj	repressor,as
R4831	T16983	T16982	amod	transcriptional,repressor
R4832	T16984	T16976	cc	and,functions
R4833	T16985	T16986	mark	that,is
R4834	T16986	T16976	conj	is,functions
R4835	T16987	T16988	det	the,activity
R4836	T16988	T16986	nsubj	activity,is
R4837	T16989	T16988	compound	repression,activity
R4838	T16990	T16988	prep	of,activity
R4839	T16991	T16992	compound	mr,s
R4840	T16992	T16990	pobj	s,of
R4841	T16993	T16992	punct	-,s
R4842	T16994	T16986	neg	not,is
R4843	T16995	T16986	prep	due,is
R4844	T16996	T16995	prep	to,due
R4845	T16997	T16998	det	a,interaction
R4846	T16998	T16996	pobj	interaction,to
R4847	T16999	T16998	amod	homophilic,interaction
R4848	T17000	T16998	prep	through,interaction
R4849	T17001	T17002	poss	its,domain
R4850	T17002	T17000	pobj	domain,through
R4851	T17003	T17002	compound	SAM,domain
R4852	T17004	T16986	cc	but,is
R4853	T17005	T16986	conj	to,is
R4854	T17006	T17007	det	the,region
R4855	T17007	T17005	pobj	region,to
R4856	T17008	T17009	npadvmod	C,terminal
R4857	T17009	T17007	amod	terminal,region
R4858	T17010	T17009	punct	-,terminal
R4859	T17011	T17012	punct	(,463
R4860	T17012	T17007	parataxis	463,region
R4861	T17013	T17012	nmod	amino,463
R4862	T17014	T17012	nmod	acids,463
R4863	T17015	T17012	prep	to,463
R4864	T17016	T17015	pobj	542,to
R4865	T17017	T17012	punct	),463
R4866	T17018	T16970	punct	.,suggest
R8157	T28701	T28702	nmod	mr,s
R8158	T28702	T28704	nmod	s,nucleotide
R8159	T28703	T28702	punct	-,s
R8160	T28704	T28705	nmod	nucleotide,sequences
R8161	T28706	T28704	cc	and,nucleotide
R8162	T28707	T28708	compound	amino,acid
R8163	T28708	T28704	conj	acid,nucleotide
R8164	T28709	T28705	punct	.,sequences
R8165	T28711	T28712	punct	(,A
R8166	T28712	T28713	meta	A,sequences
R8167	T28714	T28712	punct	),A
R8168	T28715	T28716	nmod	mr,s
R8169	T28716	T28718	nmod	s,nucleotide
R8170	T28717	T28716	punct	-,s
R8171	T28718	T28713	nmod	nucleotide,sequences
R8172	T28719	T28718	cc	and,nucleotide
R8173	T28720	T28721	compound	amino,acids
R8174	T28721	T28718	conj	acids,nucleotide
R8175	T28722	T28713	punct	.,sequences
R8176	T28724	T28725	amod	Boxed,acids
R8177	T28725	T28727	nsubj	acids,are
R8178	T28726	T28725	compound	amino,acids
R8179	T28728	T28729	det	the,sequence
R8180	T28729	T28727	attr	sequence,are
R8181	T28730	T28731	compound	SAM,domain
R8182	T28731	T28729	compound	domain,sequence
R8183	T28732	T28727	cc	and,are
R8184	T28733	T28734	det	the,box
R8185	T28734	T28736	nsubj	box,indicates
R8186	T28735	T28734	amod	dashed,box
R8187	T28736	T28727	conj	indicates,are
R8188	T28737	T28738	det	a,signal
R8189	T28738	T28736	dobj	signal,indicates
R8190	T28739	T28738	amod	putative,signal
R8191	T28740	T28741	amod	nuclear,localization
R8192	T28741	T28738	compound	localization,signal
R8193	T28742	T28736	punct	.,indicates
R8194	T28744	T28745	det	The,underline
R8195	T28745	T28746	nsubj	underline,indicates
R8196	T28747	T28748	det	a,signal
R8197	T28748	T28746	dobj	signal,indicates
R8198	T28749	T28748	amod	putative,signal
R8199	T28750	T28751	compound	polyadenylation,termination
R8200	T28751	T28748	compound	termination,signal
R8201	T28752	T28746	punct	.,indicates
R8202	T28754	T28755	punct	(,B
R8203	T28755	T28756	meta	B,Alignment
R8204	T28757	T28755	punct	),B
R8205	T28758	T28756	prep	of,Alignment
R8206	T28759	T28760	compound	SAM,domain
R8207	T28760	T28761	compound	domain,sequences
R8208	T28761	T28758	pobj	sequences,of
R8209	T28762	T28761	prep	for,sequences
R8210	T28763	T28764	compound	SAM,domain
R8211	T28764	T28765	npadvmod	domain,containing
R8212	T28765	T28767	amod	containing,proteins
R8213	T28766	T28765	punct	-,containing
R8214	T28767	T28762	pobj	proteins,for
R8215	T28768	T28756	punct	.,Alignment
R8216	T28770	T28771	det	The,helices
R8217	T28771	T28774	nsubjpass	helices,marked
R8218	T28772	T28771	nummod	five,helices
R8219	T28773	T28771	compound	alpha,helices
R8220	T28775	T28774	auxpass	are,marked
R8221	T28776	T28774	oprd	H1,marked
R8222	T28777	T28778	punct	-,H5
R8223	T28778	T28776	prep	H5,H1
R8224	T28779	T28774	punct	.,marked
R8225	T28781	T28782	amod	Conserved,residues
R8226	T28782	T28785	nsubjpass	residues,shown
R8227	T28783	T28784	compound	amino,acid
R8228	T28784	T28782	compound	acid,residues
R8229	T28786	T28785	auxpass	are,shown
R8230	T28787	T28785	prep	with,shown
R8231	T28788	T28789	det	a,shadow
R8232	T28789	T28787	pobj	shadow,with
R8233	T28790	T28789	amod	dark,shadow
R8234	T28791	T28785	cc	and,shown
R8235	T28792	T28793	advmod	functionally,similar
R8236	T28793	T28794	amod	similar,residues
R8237	T28794	T28795	nsubjpass	residues,shown
R8238	T28795	T28785	conj	shown,shown
R8239	T28796	T28795	auxpass	are,shown
R8240	T28797	T28795	prep	with,shown
R8241	T28798	T28799	det	a,shadow
R8242	T28799	T28797	pobj	shadow,with
R8243	T28800	T28799	amod	light,shadow
R8244	T28801	T28795	punct	.,shown
R8245	T28803	T28804	det	The,sites
R8246	T28804	T28805	nsubjpass	sites,indicated
R8247	T28806	T28807	dep	that,targeted
R8248	T28807	T28804	relcl	targeted,sites
R8249	T28808	T28807	auxpass	were,targeted
R8250	T28809	T28807	prep	for,targeted
R8251	T28810	T28809	pobj	mutagenesis,for
R8252	T28811	T28805	auxpass	are,indicated
R8253	T28812	T28805	prep	by,indicated
R8254	T28813	T28812	pobj	arrows,by
R8255	T28814	T28805	punct	.,indicated
R8256	T28816	T28817	punct	(,C
R8257	T28817	T28818	meta	C,tree
R8258	T28819	T28817	punct	),C
R8259	T28820	T28818	amod	Phylogenetic,tree
R8260	T28821	T28818	prep	of,tree
R8261	T28822	T28823	compound	SAM,domain
R8262	T28823	T28824	npadvmod	domain,containing
R8263	T28824	T28826	amod	containing,proteins
R8264	T28825	T28824	punct	-,containing
R8265	T28826	T28821	pobj	proteins,of
R8266	T28827	T28818	punct	.,tree
R8267	T28829	T28830	compound	Amino,acid
R8268	T28830	T28831	compound	acid,sequences
R8269	T28831	T28832	nsubjpass	sequences,analyzed
R8270	T28833	T28832	auxpass	were,analyzed
R8271	T28834	T28832	prep	by,analyzed
R8272	T28835	T28836	det	the,method
R8273	T28836	T28834	pobj	method,by
R8274	T28837	T28838	npadvmod	neighbor,joining
R8275	T28838	T28836	amod	joining,method
R8276	T28839	T28838	punct	-,joining
R8277	T28840	T28832	prep	in,analyzed
R8278	T28841	T28840	pobj	MacVector,in
R8279	T28842	T28841	nummod	7.2,MacVector
R8280	T28843	T28832	punct	.,analyzed
R8281	T28845	T28846	compound	Branch,lengths
R8282	T28846	T28847	nsubj	lengths,reflect
R8283	T28848	T28849	det	the,number
R8284	T28849	T28847	dobj	number,reflect
R8285	T28850	T28849	compound	mean,number
R8286	T28851	T28849	prep	of,number
R8287	T28852	T28851	pobj	substitutions,of
R8288	T28853	T28852	prep	per,substitutions
R8289	T28854	T28853	pobj	site,per
R8290	T28855	T28847	punct	.,reflect
R8291	T28857	T28858	punct	(,D
R8292	T28858	T28859	meta	D,comparison
R8293	T28860	T28858	punct	),D
R8294	T28861	T28859	amod	Schematic,comparison
R8295	T28862	T28859	prep	of,comparison
R8296	T28863	T28864	det	the,sequences
R8297	T28864	T28862	pobj	sequences,of
R8298	T28865	T28866	compound	amino,acid
R8299	T28866	T28864	compound	acid,sequences
R8300	T28867	T28864	prep	for,sequences
R8301	T28868	T28869	nmod	mouse,proteins
R8302	T28869	T28867	pobj	proteins,for
R8303	T28870	T28868	punct	", ",mouse
R8304	T28871	T28868	conj	rat,mouse
R8305	T28872	T28871	punct	", ",rat
R8306	T28873	T28871	amod	human,rat
R8307	T28874	T28871	punct	", ",rat
R8308	T28875	T28871	conj	chick,rat
R8309	T28876	T28875	cc	and,chick
R8310	T28877	T28875	conj	zebrafish,chick
R8311	T28878	T28879	compound	mr,s
R8312	T28879	T28869	compound	s,proteins
R8313	T28880	T28879	punct	-,s
R8314	T28881	T28859	punct	.,comparison
R8315	T28883	T28884	det	The,similarity
R8316	T28884	T28886	nsubjpass	similarity,shown
R8317	T28885	T28884	compound	percent,similarity
R8318	T28887	T28884	prep	of,similarity
R8319	T28888	T28889	det	the,domains
R8320	T28889	T28887	pobj	domains,of
R8321	T28890	T28889	compound	SAM,domains
R8322	T28891	T28889	cc	and,domains
R8323	T28892	T28893	amod	other,regions
R8324	T28893	T28889	conj	regions,domains
R8325	T28894	T28884	prep	to,similarity
R8326	T28895	T28896	det	the,regions
R8327	T28896	T28894	pobj	regions,to
R8328	T28897	T28896	amod	corresponding,regions
R8329	T28898	T28896	prep	of,regions
R8330	T28899	T28900	det	the,protein
R8331	T28900	T28898	pobj	protein,of
R8332	T28901	T28900	compound	mouse,protein
R8333	T28902	T28886	auxpass	is,shown
R8334	T28903	T28886	punct	.,shown
R8335	T28905	T28906	amod	Overall,similarity
R8336	T28906	T28908	nsubjpass	similarity,shown
R8337	T28907	T28906	compound	sequence,similarity
R8338	T28909	T28906	prep	with,similarity
R8339	T28910	T28911	det	the,protein
R8340	T28911	T28909	pobj	protein,with
R8341	T28912	T28911	compound	mouse,protein
R8342	T28913	T28908	auxpass	is,shown
R8343	T28914	T28908	prep	on,shown
R8344	T28915	T28916	det	the,right
R8345	T28916	T28914	pobj	right,on
R8346	T28917	T28908	punct	.,shown
R8351	T29458	T29459	compound	mr,s
R8352	T29459	T29461	compound	s,expression
R8353	T29460	T29459	punct	-,s
R8354	T29462	T29461	prep	in,expression
R8355	T29463	T29464	amod	developing,retina
R8356	T29464	T29462	pobj	retina,in
R8357	T29465	T29464	compound	mouse,retina
R8358	T29466	T29464	cc	and,retina
R8359	T29467	T29468	amod	pineal,gland
R8360	T29468	T29464	conj	gland,retina
R8361	T29469	T29461	punct	.,expression
R8362	T29471	T29472	punct	(,A
R8363	T29472	T29473	meta	A,expression
R8364	T29474	T29472	punct	-,A
R8365	T29475	T29472	dep	G,A
R8366	T29476	T29472	punct	),A
R8367	T29477	T29478	compound	mr,s
R8368	T29478	T29473	compound	s,expression
R8369	T29479	T29478	punct	-,s
R8370	T29480	T29473	prep	during,expression
R8371	T29481	T29480	pobj	development,during
R8372	T29482	T29481	prep	of,development
R8373	T29483	T29484	det	the,retina
R8374	T29484	T29482	pobj	retina,of
R8375	T29485	T29484	compound	mouse,retina
R8376	T29486	T29473	punct	.,expression
R8377	T29488	T29489	det	The,signal
R8378	T29489	T29493	nsubjpass	signal,detected
R8379	T29490	T29491	advmod	in,situ
R8380	T29491	T29489	amod	situ,signal
R8381	T29492	T29489	compound	hybridization,signal
R8382	T29494	T29489	prep	of,signal
R8383	T29495	T29496	compound	mr,s
R8384	T29496	T29494	pobj	s,of
R8385	T29497	T29496	punct	-,s
R8386	T29498	T29493	auxpass	was,detected
R8387	T29499	T29493	neg	not,detected
R8388	T29500	T29493	prep	at,detected
R8389	T29501	T29500	pobj	E13,at
R8390	T29502	T29503	punct	(,A
R8391	T29503	T29493	parataxis	A,detected
R8392	T29504	T29503	punct	),A
R8393	T29505	T29493	punct	.,detected
R8394	T29507	T29508	det	The,signal
R8395	T29508	T29509	nsubjpass	signal,detected
R8396	T29510	T29511	punct	(,arrow
R8397	T29511	T29508	parataxis	arrow,signal
R8398	T29512	T29511	punct	),arrow
R8399	T29513	T29509	auxpass	was,detected
R8400	T29514	T29509	advmod	first,detected
R8401	T29515	T29509	prep	in,detected
R8402	T29516	T29517	det	the,aspect
R8403	T29517	T29515	pobj	aspect,in
R8404	T29518	T29517	amod	outer,aspect
R8405	T29519	T29517	prep	of,aspect
R8406	T29520	T29519	pobj	NBL,of
R8407	T29521	T29509	prep	at,detected
R8408	T29522	T29521	pobj	E18,at
R8409	T29523	T29524	punct	(,B
R8410	T29524	T29509	parataxis	B,detected
R8411	T29525	T29524	punct	),B
R8412	T29526	T29509	punct	.,detected
R8413	T29528	T29529	det	A,signal
R8414	T29529	T29534	nsubjpass	signal,detected
R8415	T29530	T29529	amod	strong,signal
R8416	T29531	T29532	compound	mr,s
R8417	T29532	T29529	compound	s,signal
R8418	T29533	T29532	punct	-,s
R8419	T29535	T29534	auxpass	was,detected
R8420	T29536	T29534	prep	in,detected
R8421	T29537	T29538	amod	outer,layer
R8422	T29538	T29536	pobj	layer,in
R8423	T29539	T29538	prep	of,layer
R8424	T29540	T29541	det	the,retina
R8425	T29541	T29539	pobj	retina,of
R8426	T29542	T29534	prep	at,detected
R8427	T29543	T29542	pobj	P3,at
R8428	T29544	T29545	punct	-,P6
R8429	T29545	T29543	prep	P6,P3
R8430	T29546	T29534	punct	", ",detected
R8431	T29547	T29534	cc	and,detected
R8432	T29548	T29549	advmod	then,decreased
R8433	T29549	T29534	conj	decreased,detected
R8434	T29550	T29551	det	the,signal
R8435	T29551	T29549	nsubj	signal,decreased
R8436	T29552	T29549	prep	in,decreased
R8437	T29553	T29554	det	the,retina
R8438	T29554	T29552	pobj	retina,in
R8439	T29555	T29554	amod	adult,retina
R8440	T29556	T29557	punct	(,E
R8441	T29557	T29549	parataxis	E,decreased
R8442	T29558	T29559	punct	-,G
R8443	T29559	T29557	prep	G,E
R8444	T29560	T29557	punct	),E
R8445	T29561	T29549	punct	.,decreased
R8446	T29563	T29564	nsubjpass	Control,shown
R8447	T29565	T29563	prep	with,Control
R8448	T29566	T29567	det	the,probe
R8449	T29567	T29565	pobj	probe,with
R8450	T29568	T29567	compound	sense,probe
R8451	T29569	T29563	prep	in,Control
R8452	T29570	T29571	compound	E18,retina
R8453	T29571	T29569	pobj	retina,in
R8454	T29572	T29564	auxpass	is,shown
R8455	T29573	T29574	punct	(,C
R8456	T29574	T29564	parataxis	C,shown
R8457	T29575	T29574	punct	),C
R8458	T29576	T29564	punct	.,shown
R8459	T29578	T29579	compound	Scale,bar
R8460	T29580	T29579	punct	", ",bar
R8461	T29581	T29582	nummod	100,μm
R8462	T29582	T29579	appos	μm,bar
R8463	T29583	T29579	punct	.,bar
R8464	T29585	T29586	punct	(,H
R8465	T29586	T29587	meta	H,analysis
R8466	T29588	T29586	punct	),H
R8467	T29589	T29590	compound	Northern,blot
R8468	T29590	T29587	compound	blot,analysis
R8469	T29591	T29587	prep	of,analysis
R8470	T29592	T29593	compound	mr,s
R8471	T29593	T29595	compound	s,expression
R8472	T29594	T29593	punct	-,s
R8473	T29595	T29591	pobj	expression,of
R8474	T29596	T29595	prep	in,expression
R8475	T29597	T29598	amod	adult,organs
R8476	T29598	T29596	pobj	organs,in
R8477	T29599	T29598	compound	mouse,organs
R8478	T29600	T29587	punct	.,analysis
R8479	T29602	T29603	det	The,arrow
R8480	T29603	T29604	nsubj	arrow,corresponds
R8481	T29605	T29604	prep	to,corresponds
R8482	T29606	T29607	compound	2.2kb,transcript
R8483	T29607	T29605	pobj	transcript,to
R8484	T29608	T29609	compound	mr,s
R8485	T29609	T29607	compound	s,transcript
R8486	T29610	T29609	punct	-,s
R8487	T29611	T29604	punct	.,corresponds
R8488	T29613	T29614	punct	(,I
R8489	T29614	T29615	meta	I,analysis
R8490	T29616	T29614	punct	),I
R8491	T29617	T29618	compound	RT,PCR
R8492	T29618	T29615	compound	PCR,analysis
R8493	T29619	T29618	punct	-,PCR
R8494	T29620	T29615	prep	of,analysis
R8495	T29621	T29622	amod	total,RNAs
R8496	T29622	T29620	pobj	RNAs,of
R8497	T29623	T29622	acl	extracted,RNAs
R8498	T29624	T29623	prep	from,extracted
R8499	T29625	T29626	nmod	E13,embryo
R8500	T29626	T29624	pobj	embryo,from
R8501	T29627	T29626	amod	whole,embryo
R8502	T29628	T29626	punct	", ",embryo
R8503	T29629	T29630	nmod	P0,body
R8504	T29630	T29626	conj	body,embryo
R8505	T29631	T29630	amod	whole,body
R8506	T29632	T29630	punct	(,body
R8507	T29633	T29630	prep	except,body
R8508	T29634	T29633	prep	for,except
R8509	T29635	T29636	det	the,eye
R8510	T29636	T29634	pobj	eye,for
R8511	T29637	T29630	punct	),body
R8512	T29638	T29630	punct	", ",body
R8513	T29639	T29640	compound	P7,retina
R8514	T29640	T29630	conj	retina,body
R8515	T29641	T29640	punct	", ",retina
R8516	T29642	T29643	nmod	P7,gland
R8517	T29643	T29640	conj	gland,retina
R8518	T29644	T29643	amod	pineal,gland
R8519	T29645	T29643	punct	", ",gland
R8520	T29646	T29647	compound	P7,brain
R8521	T29647	T29643	conj	brain,gland
R8522	T29648	T29647	punct	", ",brain
R8523	T29649	T29650	compound	P7,liver
R8524	T29650	T29647	conj	liver,brain
R8525	T29651	T29650	punct	", ",liver
R8526	T29652	T29653	amod	adult,retina
R8527	T29653	T29650	conj	retina,liver
R8528	T29654	T29653	punct	", ",retina
R8529	T29655	T29656	amod	adult,gland
R8530	T29656	T29653	conj	gland,retina
R8531	T29657	T29656	amod	pineal,gland
R8532	T29658	T29656	punct	", ",gland
R8533	T29659	T29660	amod	adult,brain
R8534	T29660	T29656	conj	brain,gland
R8535	T29661	T29660	cc	and,brain
R8536	T29662	T29663	amod	adult,liver
R8537	T29663	T29660	conj	liver,brain
R8538	T29664	T29623	punct	", ",extracted
R8539	T29665	T29623	advmod	respectively,extracted
R8540	T29666	T29615	punct	.,analysis
R8541	T29669	T29668	punct	", ",RPE
R8542	T29670	T29671	amod	retinal,epithelium
R8543	T29671	T29668	appos	epithelium,RPE
R8544	T29672	T29671	compound	pigment,epithelium
R8545	T29673	T29668	punct	;,RPE
R8546	T29674	T29668	appos	NR,RPE
R8547	T29675	T29674	punct	", ",NR
R8548	T29676	T29677	amod	neural,retina
R8549	T29677	T29674	appos	retina,NR
R8550	T29678	T29668	punct	;,RPE
R8551	T29679	T29668	appos	NBL,RPE
R8552	T29680	T29679	punct	", ",NBL
R8553	T29681	T29682	amod	neuroblastic,layer
R8554	T29682	T29679	appos	layer,NBL
R8555	T29683	T29668	punct	;,RPE
R8556	T29684	T29668	appos	GCL,RPE
R8557	T29685	T29684	punct	", ",GCL
R8558	T29686	T29687	compound	ganglion,cell
R8559	T29687	T29688	compound	cell,layer
R8560	T29688	T29684	appos	layer,GCL
R8561	T29689	T29668	punct	;,RPE
R8562	T29690	T29668	appos	ONL,RPE
R8563	T29691	T29690	punct	", ",ONL
R8564	T29692	T29693	amod	outer,layer
R8565	T29693	T29690	appos	layer,ONL
R8566	T29694	T29693	amod	nuclear,layer
R8567	T29695	T29668	punct	;,RPE
R8568	T29696	T29668	appos	INL,RPE
R8569	T29697	T29696	punct	", ",INL
R8570	T29698	T29699	amod	inner,layer
R8571	T29699	T29696	appos	layer,INL
R8572	T29700	T29699	amod	nuclear,layer
R8573	T29701	T29668	punct	.,RPE
R8574	T30251	T30252	det	The,transcription
R8575	T30252	T30253	nsubjpass	transcription,regulated
R8576	T30254	T30252	prep	of,transcription
R8577	T30255	T30256	compound	mr,s
R8578	T30256	T30254	pobj	s,of
R8579	T30257	T30256	punct	-,s
R8580	T30258	T30253	auxpass	is,regulated
R8581	T30259	T30253	agent	by,regulated
R8582	T30260	T30259	pobj	Crx,by
R8583	T30261	T30253	punct	.,regulated
R8584	T30263	T30264	punct	(,A
R8585	T30264	T30265	meta	A,performed
R8586	T30266	T30264	punct	", ",A
R8587	T30267	T30264	dep	B,A
R8588	T30268	T30264	punct	),A
R8589	T30269	T30270	advmod	In,situ
R8590	T30270	T30271	amod	situ,hybridization
R8591	T30271	T30265	nsubjpass	hybridization,performed
R8592	T30272	T30271	acl	using,hybridization
R8593	T30273	T30274	det	a,probe
R8594	T30274	T30272	dobj	probe,using
R8595	T30275	T30274	prep	for,probe
R8596	T30276	T30277	compound	mouse,s
R8597	T30277	T30275	pobj	s,for
R8598	T30278	T30277	compound	mr,s
R8599	T30279	T30277	punct	-,s
R8600	T30280	T30265	auxpass	was,performed
R8601	T30281	T30265	prep	on,performed
R8602	T30282	T30283	det	the,retinas
R8603	T30283	T30281	pobj	retinas,on
R8604	T30284	T30285	amod	wild,type
R8605	T30285	T30283	nmod	type,retinas
R8606	T30286	T30285	punct	-,type
R8607	T30287	T30288	punct	(,A
R8608	T30288	T30285	parataxis	A,type
R8609	T30289	T30288	punct	),A
R8610	T30290	T30285	cc	and,type
R8611	T30291	T30292	compound	Crx,KO
R8612	T30292	T30285	conj	KO,type
R8613	T30293	T30294	punct	(,B
R8614	T30294	T30283	parataxis	B,retinas
R8615	T30295	T30294	punct	),B
R8616	T30296	T30265	prep	at,performed
R8617	T30297	T30296	pobj	P5,at
R8618	T30298	T30265	punct	.,performed
R8619	T30300	T30301	compound	mr,s
R8620	T30301	T30303	compound	s,expression
R8621	T30302	T30301	punct	-,s
R8622	T30303	T30304	nsubjpass	expression,reduced
R8623	T30305	T30304	auxpass	was,reduced
R8624	T30306	T30304	advmod	drastically,reduced
R8625	T30307	T30304	prep	in,reduced
R8626	T30308	T30309	det	the,retina
R8627	T30309	T30307	pobj	retina,in
R8628	T30310	T30311	compound	Crx,KO
R8629	T30311	T30309	compound	KO,retina
R8630	T30312	T30313	punct	(,B
R8631	T30313	T30309	parataxis	B,retina
R8632	T30314	T30313	punct	),B
R8633	T30315	T30304	punct	.,reduced
R8634	T30317	T30318	compound	Scale,bar
R8635	T30319	T30318	punct	", ",bar
R8636	T30320	T30321	nummod	100,μm
R8637	T30321	T30318	appos	μm,bar
R8638	T30322	T30318	punct	.,bar
R8639	T30324	T30325	punct	(,C
R8640	T30325	T30326	meta	C,analysis
R8641	T30327	T30325	punct	),C
R8642	T30328	T30329	compound	RT,PCR
R8643	T30329	T30326	compound	PCR,analysis
R8644	T30330	T30329	punct	-,PCR
R8645	T30331	T30326	prep	of,analysis
R8646	T30332	T30333	amod	total,RNAs
R8647	T30333	T30331	pobj	RNAs,of
R8648	T30334	T30333	acl	extracted,RNAs
R8649	T30335	T30334	prep	from,extracted
R8650	T30336	T30337	det	the,glands
R8651	T30337	T30335	pobj	glands,from
R8652	T30338	T30337	amod	pineal,glands
R8653	T30339	T30337	prep	of,glands
R8654	T30340	T30341	nmod	P5,mouse
R8655	T30341	T30339	pobj	mouse,of
R8656	T30342	T30343	amod	wild,type
R8657	T30343	T30341	nmod	type,mouse
R8658	T30344	T30343	punct	-,type
R8659	T30345	T30343	cc	and,type
R8660	T30346	T30347	compound	Crx,KO
R8661	T30347	T30343	conj	KO,type
R8662	T30348	T30326	punct	.,analysis
R8663	T30350	T30351	det	The,lanes
R8664	T30351	T30355	nsubj	lanes,show
R8665	T30352	T30351	amod	upper,lanes
R8666	T30353	T30352	cc	and,upper
R8667	T30354	T30352	conj	lower,upper
R8668	T30356	T30357	compound	PCR,products
R8669	T30357	T30355	dobj	products,show
R8670	T30358	T30357	acl	amplified,products
R8671	T30359	T30358	agent	by,amplified
R8672	T30360	T30361	det	the,pairs
R8673	T30361	T30359	pobj	pairs,by
R8674	T30362	T30361	compound	primer,pairs
R8675	T30363	T30361	amod	specific,pairs
R8676	T30364	T30363	prep	for,specific
R8677	T30365	T30366	compound	mr,s
R8678	T30366	T30364	pobj	s,for
R8679	T30367	T30366	punct	-,s
R8680	T30368	T30366	cc	and,s
R8681	T30369	T30370	compound	G3PDH,cDNAs
R8682	T30370	T30366	conj	cDNAs,s
R8683	T30371	T30358	punct	", ",amplified
R8684	T30372	T30358	advmod	respectively,amplified
R8685	T30373	T30355	punct	.,show
R8686	T30375	T30376	nsubjpass	Water,used
R8687	T30377	T30376	auxpass	was,used
R8688	T30378	T30376	prep	for,used
R8689	T30379	T30380	compound	control,reaction
R8690	T30380	T30378	pobj	reaction,for
R8691	T30381	T30382	compound	RT,PCR
R8692	T30382	T30380	compound	PCR,reaction
R8693	T30383	T30382	punct	-,PCR
R8694	T30384	T30376	punct	.,used
R8695	T30386	T30387	punct	(,D
R8696	T30387	T30388	meta	D,transactivates
R8697	T30389	T30387	punct	-,D
R8698	T30390	T30387	dep	F,D
R8699	T30391	T30387	punct	),D
R8700	T30392	T30388	nsubj	Crx,transactivates
R8701	T30393	T30394	compound	mr,s
R8702	T30394	T30396	compound	s,transcription
R8703	T30395	T30394	punct	-,s
R8704	T30396	T30388	dobj	transcription,transactivates
R8705	T30397	T30388	punct	.,transactivates
R8706	T30399	T30400	compound	Reporter,plasmids
R8707	T30400	T30401	nsubjpass	plasmids,shown
R8708	T30402	T30400	prep	for,plasmids
R8709	T30403	T30404	det	the,assay
R8710	T30404	T30402	pobj	assay,for
R8711	T30405	T30404	compound	luciferase,assay
R8712	T30406	T30401	auxpass	are,shown
R8713	T30407	T30401	punct	.,shown
R8714	T30409	T30410	amod	Blue,boxes
R8715	T30410	T30411	nsubj	boxes,represent
R8716	T30412	T30413	compound	Crx,sites
R8717	T30413	T30411	dobj	sites,represent
R8718	T30414	T30413	compound	binding,sites
R8719	T30415	T30416	punct	(,D
R8720	T30416	T30411	parataxis	D,represent
R8721	T30417	T30416	punct	),D
R8722	T30418	T30411	punct	.,represent
R8723	T30420	T30421	amod	Relative,activity
R8724	T30421	T30422	nsubjpass	activity,indicated
R8725	T30423	T30421	prep	of,activity
R8726	T30424	T30425	det	the,luciferase
R8727	T30425	T30423	pobj	luciferase,of
R8728	T30426	T30422	auxpass	is,indicated
R8729	T30427	T30428	advmod	when,co-transfected
R8730	T30428	T30422	advcl	co-transfected,indicated
R8731	T30429	T30428	nsubjpass	Pro1.2k,co-transfected
R8732	T30430	T30428	auxpass	was,co-transfected
R8733	T30431	T30428	prep	with,co-transfected
R8734	T30432	T30431	pobj	Crx,with
R8735	T30433	T30432	punct	", ",Crx
R8736	T30434	T30432	conj	Otx2,Crx
R8737	T30435	T30434	punct	", ",Otx2
R8738	T30436	T30434	conj	Nrl,Otx2
R8739	T30437	T30436	punct	", ",Nrl
R8740	T30438	T30436	cc	and,Nrl
R8741	T30439	T30440	nmod	Crx,Nrl
R8742	T30440	T30436	conj	Nrl,Nrl
R8743	T30441	T30440	punct	+,Nrl
R8744	T30442	T30428	punct	", ",co-transfected
R8745	T30443	T30428	advmod	respectively,co-transfected
R8746	T30444	T30445	punct	(,E
R8747	T30445	T30428	parataxis	E,co-transfected
R8748	T30446	T30445	punct	),E
R8749	T30447	T30422	punct	.,indicated
R8750	T30449	T30450	compound	Fold,activation
R8751	T30450	T30451	nsubjpass	activation,indicated
R8752	T30452	T30451	auxpass	is,indicated
R8753	T30453	T30454	advmod	when,co-transfected
R8754	T30454	T30451	advcl	co-transfected,indicated
R8755	T30455	T30454	nsubjpass	Pro1.2k,co-transfected
R8756	T30456	T30455	punct	", ",Pro1.2k
R8757	T30457	T30455	conj	mut1259,Pro1.2k
R8758	T30458	T30457	punct	", ",mut1259
R8759	T30459	T30457	conj	mut198,mut1259
R8760	T30460	T30459	punct	", ",mut198
R8761	T30461	T30459	conj	mut72,mut198
R8762	T30462	T30461	cc	and,mut72
R8763	T30463	T30464	compound	mut,all
R8764	T30464	T30461	conj	all,mut72
R8765	T30465	T30454	auxpass	were,co-transfected
R8766	T30466	T30454	prep	with,co-transfected
R8767	T30467	T30468	det	the,vector
R8768	T30468	T30466	pobj	vector,with
R8769	T30469	T30468	compound	Crx,vector
R8770	T30470	T30468	compound	expression,vector
R8771	T30471	T30472	punct	(,Crx
R8772	T30472	T30468	parataxis	Crx,vector
R8773	T30473	T30472	punct	+,Crx
R8774	T30474	T30472	punct	),Crx
R8775	T30475	T30468	cc	or,vector
R8776	T30476	T30477	det	the,vector
R8777	T30477	T30468	conj	vector,vector
R8778	T30478	T30477	amod	empty,vector
R8779	T30479	T30480	punct	(,Crx
R8780	T30480	T30477	parataxis	Crx,vector
R8781	T30481	T30480	punct	-,Crx
R8782	T30482	T30480	punct	),Crx
R8783	T30483	T30454	prep	into,co-transfected
R8784	T30484	T30485	compound	HEK293T,cells
R8785	T30485	T30483	pobj	cells,into
R8786	T30486	T30487	punct	(,F
R8787	T30487	T30451	parataxis	F,indicated
R8788	T30488	T30487	punct	),F
R8789	T30489	T30451	punct	.,indicated
R8790	T30491	T30492	compound	Error,bars
R8791	T30492	T30493	nsubj	bars,represent
R8792	T30494	T30495	amod	standard,error
R8793	T30495	T30493	dobj	error,represent
R8794	T30496	T30495	prep	of,error
R8795	T30497	T30496	pobj	mean,of
R8796	T30498	T30493	punct	.,represent
R8797	T30893	T30892	prep	of,Summary
R8798	T30894	T30895	nmod	yeast,screening
R8799	T30895	T30893	pobj	screening,of
R8800	T30896	T30897	nummod	two,hybrid
R8801	T30897	T30895	compound	hybrid,screening
R8802	T30898	T30897	punct	-,hybrid
R8803	T30899	T30895	cc	and,screening
R8804	T30900	T30901	compound	GAL4,assay
R8805	T30901	T30895	conj	assay,screening
R8806	T30902	T30892	punct	.,Summary
R8807	T30904	T30905	punct	(,A
R8808	T30905	T30906	meta	A,indicated
R8809	T30907	T30905	punct	),A
R8810	T30908	T30909	amod	Full,length
R8811	T30909	T30911	compound	length,s
R8812	T30910	T30909	punct	-,length
R8813	T30911	T30906	nsubjpass	s,indicated
R8814	T30912	T30911	compound	mr,s
R8815	T30913	T30911	punct	-,s
R8816	T30914	T30911	prep	as,s
R8817	T30915	T30916	det	a,bait
R8818	T30916	T30914	pobj	bait,as
R8819	T30917	T30916	acl	used,bait
R8820	T30918	T30917	prep	in,used
R8821	T30919	T30920	det	the,screening
R8822	T30920	T30918	pobj	screening,in
R8823	T30921	T30920	nmod	yeast,screening
R8824	T30922	T30923	nummod	two,hybrid
R8825	T30923	T30920	compound	hybrid,screening
R8826	T30924	T30923	punct	-,hybrid
R8827	T30925	T30911	cc	and,s
R8828	T30926	T30927	amod	positive,clones
R8829	T30927	T30911	conj	clones,s
R8830	T30928	T30906	auxpass	are,indicated
R8831	T30929	T30906	punct	.,indicated
R8832	T30932	T30933	mark	that,contain
R8833	T30933	T30931	ccomp	contain,Note
R8834	T30934	T30933	nsubj	all,contain
R8835	T30935	T30934	prep	of,all
R8836	T30936	T30937	nummod	five,clones
R8837	T30937	T30935	pobj	clones,of
R8838	T30938	T30939	compound	mr,s
R8839	T30939	T30937	compound	s,clones
R8840	T30940	T30939	punct	-,s
R8841	T30941	T30937	acl	identified,clones
R8842	T30942	T30941	prep	in,identified
R8843	T30943	T30944	det	the,screening
R8844	T30944	T30942	pobj	screening,in
R8845	T30945	T30946	compound	SAM,domains
R8846	T30946	T30933	dobj	domains,contain
R8847	T30947	T30931	punct	.,Note
R8848	T30949	T30950	punct	(,B
R8849	T30950	T30951	meta	B,diagram
R8850	T30952	T30950	punct	),B
R8851	T30953	T30951	amod	Schematic,diagram
R8852	T30954	T30951	prep	of,diagram
R8853	T30955	T30956	det	the,proteins
R8854	T30956	T30954	pobj	proteins,of
R8855	T30957	T30958	compound	mr,s
R8856	T30958	T30956	compound	s,proteins
R8857	T30959	T30958	punct	-,s
R8858	T30960	T30956	compound	fusion,proteins
R8859	T30961	T30956	acl	used,proteins
R8860	T30962	T30961	prep	in,used
R8861	T30963	T30964	det	the,assay
R8862	T30964	T30962	pobj	assay,in
R8863	T30965	T30964	compound	yeast,assay
R8864	T30966	T30964	compound	GAL4,assay
R8865	T30967	T30951	punct	.,diagram
R8866	T30969	T30970	amod	Black,boxes
R8867	T30970	T30971	nsubj	boxes,represent
R8868	T30972	T30973	det	the,position
R8869	T30973	T30971	dobj	position,represent
R8870	T30974	T30973	prep	of,position
R8871	T30975	T30976	compound	SAM,domains
R8872	T30976	T30974	pobj	domains,of
R8873	T30977	T30971	punct	.,represent
R8874	T30979	T30980	amod	Relative,levels
R8875	T30980	T30981	nsubjpass	levels,shown
R8876	T30982	T30980	prep	of,levels
R8877	T30983	T30984	compound	LacZ,expression
R8878	T30984	T30982	pobj	expression,of
R8879	T30985	T30981	auxpass	are,shown
R8880	T30986	T30981	prep	on,shown
R8881	T30987	T30988	det	the,right
R8882	T30988	T30986	pobj	right,on
R8883	T30989	T30981	punct	", ",shown
R8884	T30990	T30981	advmod	respectively,shown
R8885	T30991	T30981	punct	.,shown
R8886	T30993	T30994	dep	Note,indicates
R8887	T30995	T30994	punct	;,indicates
R8888	T30996	T30997	nsubj	++,indicates
R8889	T30997	T30994	ccomp	indicates,indicates
R8890	T30998	T30999	det	an,signal
R8891	T30999	T30997	dobj	signal,indicates
R8892	T31000	T30999	amod	intense,signal
R8893	T31001	T30999	amod	blue,signal
R8894	T31002	T30999	relcl	visible,signal
R8895	T31003	T31002	prep	after,visible
R8896	T31004	T31003	pobj	12hr,after
R8897	T31005	T31004	prep	of,12hr
R8898	T31006	T31005	pobj	incubation,of
R8899	T31007	T31006	prep	at,incubation
R8900	T31008	T31009	nummod	37,°C
R8901	T31009	T31007	pobj	°C,at
R8902	T31010	T30994	punct	", ",indicates
R8903	T31011	T30994	nsubj	+,indicates
R8904	T31012	T31013	det	a,signal
R8905	T31013	T30994	dobj	signal,indicates
R8906	T31014	T31013	amod	blue,signal
R8907	T31015	T31013	relcl	visible,signal
R8908	T31016	T31015	prep	after,visible
R8909	T31017	T31016	pobj	24hr,after
R8910	T31018	T31017	prep	of,24hr
R8911	T31019	T31018	pobj	incubation,of
R8912	T31020	T30994	punct	.,indicates
R8913	T31023	T31022	punct	", ",BD
R8914	T31024	T31025	compound	binding,domain
R8915	T31025	T31022	appos	domain,BD
R8916	T31026	T31022	punct	;,BD
R8917	T31027	T31022	appos	AD,BD
R8918	T31028	T31027	punct	", ",AD
R8919	T31029	T31030	compound	activation,domain
R8920	T31030	T31027	appos	domain,AD
R8921	T31031	T31022	punct	;,BD
R8922	T31032	T31033	amod	full,s
R8923	T31033	T31022	appos	s,BD
R8924	T31034	T31033	punct	", ",s
R8925	T31035	T31036	amod	full,length
R8926	T31036	T31033	compound	length,s
R8927	T31037	T31036	punct	-,length
R8928	T31038	T31033	compound	mr,s
R8929	T31039	T31033	punct	-,s
R8930	T31040	T31022	punct	;,BD
R8931	T31041	T31022	appos	N,BD
R8932	T31042	T31041	punct	", ",N
R8933	T31043	T31044	npadvmod	N,terminal
R8934	T31044	T31046	amod	terminal,portion
R8935	T31045	T31044	punct	-,terminal
R8936	T31046	T31041	appos	portion,N
R8937	T31047	T31046	prep	of,portion
R8938	T31048	T31049	compound	mr,s
R8939	T31049	T31047	pobj	s,of
R8940	T31050	T31049	punct	-,s
R8941	T31051	T31052	punct	(,1
R8942	T31052	T31046	parataxis	1,portion
R8943	T31053	T31052	nmod	amino,1
R8944	T31054	T31052	nmod	acids,1
R8945	T31055	T31052	prep	to,1
R8946	T31056	T31055	pobj	400,to
R8947	T31057	T31052	punct	),1
R8948	T31058	T31022	punct	;,BD
R8949	T31059	T31022	appos	C,BD
R8950	T31060	T31059	punct	", ",C
R8951	T31061	T31062	npadvmod	C,terminal
R8952	T31062	T31064	amod	terminal,portion
R8953	T31063	T31062	punct	-,terminal
R8954	T31064	T31059	appos	portion,C
R8955	T31065	T31064	prep	of,portion
R8956	T31066	T31067	compound	mr,s
R8957	T31067	T31065	pobj	s,of
R8958	T31068	T31067	punct	-,s
R8959	T31069	T31070	punct	(,391
R8960	T31070	T31064	parataxis	391,portion
R8961	T31071	T31070	nmod	amino,391
R8962	T31072	T31070	nmod	acids,391
R8963	T31073	T31070	prep	to,391
R8964	T31074	T31073	pobj	542,to
R8965	T31075	T31070	punct	),391
R8966	T31076	T31022	punct	.,BD
R8967	T31407	T31408	det	The,protein
R8968	T31408	T31412	nsubj	protein,associate
R8969	T31409	T31410	compound	mr,s
R8970	T31410	T31408	compound	s,protein
R8971	T31411	T31410	punct	-,s
R8972	T31413	T31412	aux	can,associate
R8973	T31414	T31412	dep	self,associate
R8974	T31415	T31412	punct	-,associate
R8975	T31416	T31412	prep	in,associate
R8976	T31417	T31418	amod	mammalian,cells
R8977	T31418	T31416	pobj	cells,in
R8978	T31419	T31412	punct	.,associate
R8979	T31421	T31422	punct	(,A
R8980	T31422	T31423	meta	A,drawing
R8981	T31424	T31422	punct	),A
R8982	T31425	T31423	amod	Schematic,drawing
R8983	T31426	T31423	prep	of,drawing
R8984	T31427	T31428	det	the,constructs
R8985	T31428	T31426	pobj	constructs,of
R8986	T31429	T31428	acl	used,constructs
R8987	T31430	T31429	prep	for,used
R8988	T31431	T31432	compound	immunoprecipitation,assay
R8989	T31432	T31430	pobj	assay,for
R8990	T31433	T31423	punct	.,drawing
R8991	T31435	T31436	npadvmod	HA,tagged
R8992	T31436	T31438	amod	tagged,regions
R8993	T31437	T31436	punct	-,tagged
R8994	T31438	T31471	nsubjpass	regions,inserted
R8995	T31439	T31436	cc	or,tagged
R8996	T31440	T31441	npadvmod	Flag,tagged
R8997	T31441	T31436	conj	tagged,tagged
R8998	T31442	T31441	punct	-,tagged
R8999	T31443	T31444	amod	full,length
R9000	T31444	T31438	nmod	length,regions
R9001	T31445	T31444	punct	-,length
R9002	T31446	T31447	punct	(,1
R9003	T31447	T31444	parataxis	1,length
R9004	T31448	T31447	nmod	amino,1
R9005	T31449	T31447	nmod	acids,1
R9006	T31450	T31447	prep	to,1
R9007	T31451	T31450	pobj	542,to
R9008	T31452	T31447	punct	),1
R9009	T31453	T31438	punct	", ",regions
R9010	T31454	T31438	nmod	ΔSAM,regions
R9011	T31455	T31456	punct	(,1
R9012	T31456	T31454	parataxis	1,ΔSAM
R9013	T31457	T31456	nmod	amino,1
R9014	T31458	T31456	nmod	acids,1
R9015	T31459	T31456	prep	to,1
R9016	T31460	T31459	pobj	400,to
R9017	T31461	T31456	punct	),1
R9018	T31462	T31454	cc	and,ΔSAM
R9019	T31463	T31454	conj	SAM,ΔSAM
R9020	T31464	T31465	punct	(,401
R9021	T31465	T31463	parataxis	401,SAM
R9022	T31466	T31465	nmod	amino,401
R9023	T31467	T31465	nmod	acids,401
R9024	T31468	T31465	prep	to,401
R9025	T31469	T31468	pobj	542,to
R9026	T31470	T31465	punct	),401
R9027	T31472	T31471	auxpass	were,inserted
R9028	T31473	T31471	prep	into,inserted
R9029	T31474	T31475	compound	pcDNA3,vector
R9030	T31475	T31473	pobj	vector,into
R9031	T31476	T31471	punct	", ",inserted
R9032	T31477	T31471	advmod	respectively,inserted
R9033	T31478	T31471	punct	.,inserted
R9034	T31480	T31481	punct	(,B
R9035	T31481	T31482	meta	B,transfected
R9036	T31483	T31481	punct	),B
R9037	T31484	T31485	det	The,constructs
R9038	T31485	T31482	nsubjpass	constructs,transfected
R9039	T31486	T31485	acl	indicated,constructs
R9040	T31487	T31486	advmod	above,indicated
R9041	T31488	T31482	auxpass	were,transfected
R9042	T31489	T31482	prep	into,transfected
R9043	T31490	T31491	compound	HEK293T,cells
R9044	T31491	T31489	pobj	cells,into
R9045	T31492	T31482	punct	.,transfected
R9046	T31494	T31495	det	Each,lane
R9047	T31495	T31496	nsubjpass	lane,co-immunoprecipitated
R9048	T31497	T31496	auxpass	was,co-immunoprecipitated
R9049	T31498	T31496	agent	by,co-immunoprecipitated
R9050	T31499	T31500	amod	anti-Flag,antibody
R9051	T31500	T31498	pobj	antibody,by
R9052	T31501	T31496	cc	and,co-immunoprecipitated
R9053	T31502	T31496	conj	detected,co-immunoprecipitated
R9054	T31503	T31502	agent	by,detected
R9055	T31504	T31505	amod	anti-HA,antibody
R9056	T31505	T31503	pobj	antibody,by
R9057	T31506	T31496	punct	.,co-immunoprecipitated
R9058	T31508	T31509	compound	Input,lysates
R9059	T31509	T31511	nsubjpass	lysates,shown
R9060	T31510	T31509	compound	protein,lysates
R9061	T31512	T31511	auxpass	are,shown
R9062	T31513	T31511	prep	in,shown
R9063	T31514	T31515	det	the,panels
R9064	T31515	T31513	pobj	panels,in
R9065	T31516	T31515	amod	lower,panels
R9066	T31517	T31511	punct	.,shown
R9067	T31519	T31520	punct	(,C
R9068	T31520	T31521	meta	C,generated
R9069	T31522	T31520	punct	),C
R9070	T31523	T31524	npadvmod	Flag,tagged
R9071	T31524	T31526	amod	tagged,mutants
R9072	T31525	T31524	punct	-,tagged
R9073	T31526	T31521	nsubjpass	mutants,generated
R9074	T31527	T31528	nummod	two,site
R9075	T31528	T31529	npadvmod	site,directed
R9076	T31529	T31526	amod	directed,mutants
R9077	T31530	T31529	punct	-,directed
R9078	T31531	T31532	compound	mr,s
R9079	T31532	T31526	compound	s,mutants
R9080	T31533	T31532	punct	-,s
R9081	T31534	T31526	punct	", ",mutants
R9082	T31535	T31536	compound	Flag,W404A
R9083	T31536	T31526	appos	W404A,mutants
R9084	T31537	T31536	punct	-,W404A
R9085	T31538	T31536	cc	and,W404A
R9086	T31539	T31540	compound	Flag,G453A
R9087	T31540	T31536	conj	G453A,W404A
R9088	T31541	T31540	punct	-,G453A
R9089	T31542	T31521	auxpass	were,generated
R9090	T31543	T31521	cc	and,generated
R9091	T31544	T31521	conj	co-transfected,generated
R9092	T31545	T31544	prep	with,co-transfected
R9093	T31546	T31547	amod	full,HA
R9094	T31547	T31545	pobj	HA,with
R9095	T31548	T31547	punct	-,HA
R9096	T31549	T31521	punct	.,generated
R9097	T31551	T31552	det	Each,lane
R9098	T31552	T31553	nsubjpass	lane,co-immunoprecipitated
R9099	T31554	T31553	auxpass	was,co-immunoprecipitated
R9100	T31555	T31553	agent	by,co-immunoprecipitated
R9101	T31556	T31557	amod	anti-HA,antibody
R9102	T31557	T31555	pobj	antibody,by
R9103	T31558	T31553	cc	and,co-immunoprecipitated
R9104	T31559	T31553	conj	detected,co-immunoprecipitated
R9105	T31560	T31559	agent	by,detected
R9106	T31561	T31562	amod	anti-Flag,antibody
R9107	T31562	T31560	pobj	antibody,by
R9108	T31563	T31553	punct	.,co-immunoprecipitated
R9109	T31729	T31730	amod	Subcellular,localization
R9110	T31731	T31730	prep	of,localization
R9111	T31732	T31733	compound	mr,s
R9112	T31733	T31731	pobj	s,of
R9113	T31734	T31733	punct	-,s
R9114	T31735	T31730	prep	in,localization
R9115	T31736	T31737	amod	mammalian,cells
R9116	T31737	T31735	pobj	cells,in
R9117	T31738	T31730	punct	.,localization
R9118	T31740	T31741	punct	(,A
R9119	T31741	T31742	meta	A,transfected
R9120	T31743	T31741	punct	", ",A
R9121	T31744	T31741	dep	B,A
R9122	T31745	T31741	punct	),A
R9123	T31746	T31747	npadvmod	HA,tagged
R9124	T31747	T31749	amod	tagged,s
R9125	T31748	T31747	punct	-,tagged
R9126	T31749	T31742	nsubjpass	s,transfected
R9127	T31750	T31751	amod	full,length
R9128	T31751	T31749	compound	length,s
R9129	T31752	T31751	punct	-,length
R9130	T31753	T31749	compound	mr,s
R9131	T31754	T31749	punct	-,s
R9132	T31755	T31742	auxpass	was,transfected
R9133	T31756	T31742	prep	into,transfected
R9134	T31757	T31756	pobj	HEK293T,into
R9135	T31758	T31759	punct	(,B
R9136	T31759	T31742	parataxis	B,transfected
R9137	T31760	T31759	punct	),B
R9138	T31761	T31742	cc	and,transfected
R9139	T31762	T31742	conj	detected,transfected
R9140	T31763	T31762	agent	by,detected
R9141	T31764	T31765	amod	anti-HA,antibody
R9142	T31765	T31763	pobj	antibody,by
R9143	T31766	T31767	punct	(,A
R9144	T31767	T31762	parataxis	A,detected
R9145	T31768	T31767	punct	),A
R9146	T31769	T31742	punct	.,transfected
R9147	T31771	T31772	compound	Scale,bar
R9148	T31773	T31772	punct	", ",bar
R9149	T31774	T31775	nummod	20,μm
R9150	T31775	T31772	appos	μm,bar
R9151	T31776	T31772	punct	.,bar
R9152	T31778	T31779	punct	(,C
R9153	T31779	T31780	meta	C,cells
R9154	T31781	T31779	punct	-,C
R9155	T31782	T31779	dep	E,C
R9156	T31783	T31779	punct	),C
R9157	T31784	T31780	compound	HEK293T,cells
R9158	T31785	T31780	acl	immunostained,cells
R9159	T31786	T31785	prep	with,immunostained
R9160	T31787	T31788	amod	anti-HA,antibody
R9161	T31788	T31786	pobj	antibody,with
R9162	T31789	T31790	punct	(,C
R9163	T31790	T31780	parataxis	C,cells
R9164	T31791	T31790	punct	),C
R9165	T31792	T31780	punct	", ",cells
R9166	T31793	T31780	conj	DAPI,cells
R9167	T31794	T31795	punct	(,D
R9168	T31795	T31793	parataxis	D,DAPI
R9169	T31796	T31795	punct	),D
R9170	T31797	T31793	punct	", ",DAPI
R9171	T31798	T31793	cc	and,DAPI
R9172	T31799	T31800	amod	merged,image
R9173	T31800	T31793	conj	image,DAPI
R9174	T31801	T31802	punct	(,E
R9175	T31802	T31800	parataxis	E,image
R9176	T31803	T31802	punct	),E
R9177	T31804	T31800	punct	.,image
R9178	T31806	T31807	compound	Scale,bar
R9179	T31808	T31807	punct	", ",bar
R9180	T31809	T31810	nummod	50,μm
R9181	T31810	T31807	appos	μm,bar
R9182	T31811	T31807	punct	.,bar
R9183	T32444	T32445	compound	mr,s
R9184	T32445	T32447	nsubj	s,functions
R9185	T32446	T32445	punct	-,s
R9186	T32448	T32445	acl	fused,s
R9187	T32449	T32448	prep	to,fused
R9188	T32450	T32451	nmod	GAL4,domain
R9189	T32451	T32449	pobj	domain,to
R9190	T32452	T32453	npadvmod	DNA,binding
R9191	T32453	T32451	amod	binding,domain
R9192	T32454	T32447	prep	as,functions
R9193	T32455	T32456	det	a,repressor
R9194	T32456	T32454	pobj	repressor,as
R9195	T32457	T32456	amod	transcriptional,repressor
R9196	T32458	T32447	prep	in,functions
R9197	T32459	T32460	compound	HEK293T,cells
R9198	T32460	T32458	pobj	cells,in
R9199	T32461	T32447	punct	.,functions
R9200	T32463	T32464	punct	(,A
R9201	T32464	T32465	meta	A,drawing
R9202	T32466	T32464	punct	),A
R9203	T32467	T32465	amod	Schematic,drawing
R9204	T32468	T32465	prep	of,drawing
R9205	T32469	T32470	det	the,constructs
R9206	T32470	T32468	pobj	constructs,of
R9207	T32471	T32470	acl	used,constructs
R9208	T32472	T32471	prep	for,used
R9209	T32473	T32474	det	the,assay
R9210	T32474	T32472	pobj	assay,for
R9211	T32475	T32474	compound	luciferase,assay
R9212	T32476	T32465	punct	.,drawing
R9213	T32478	T32479	compound	5xGAL4,pGL3
R9214	T32479	T32481	compound	pGL3,plasmid
R9215	T32480	T32479	punct	-,pGL3
R9216	T32481	T32483	nsubjpass	plasmid,co-transfected
R9217	T32482	T32481	compound	reporter,plasmid
R9218	T32484	T32483	auxpass	was,co-transfected
R9219	T32485	T32483	prep	into,co-transfected
R9220	T32486	T32487	compound	HEK293T,cells
R9221	T32487	T32485	pobj	cells,into
R9222	T32488	T32487	prep	with,cells
R9223	T32489	T32490	compound	effector,plasmids
R9224	T32490	T32488	pobj	plasmids,with
R9225	T32491	T32490	acl	expressing,plasmids
R9226	T32492	T32493	amod	various,mutants
R9227	T32493	T32491	dobj	mutants,expressing
R9228	T32494	T32493	compound	deletion,mutants
R9229	T32495	T32493	acl	fused,mutants
R9230	T32496	T32495	prep	to,fused
R9231	T32497	T32498	compound	GAL4,DBD
R9232	T32498	T32496	pobj	DBD,to
R9233	T32499	T32498	punct	-,DBD
R9234	T32500	T32483	punct	.,co-transfected
R9235	T32502	T32503	punct	(,B
R9236	T32503	T32504	meta	B,transfected
R9237	T32505	T32503	punct	),B
R9238	T32506	T32507	amod	Various,amounts
R9239	T32507	T32504	nsubjpass	amounts,transfected
R9240	T32508	T32507	prep	of,amounts
R9241	T32509	T32510	nmod	DBD,plasmids
R9242	T32510	T32508	pobj	plasmids,of
R9243	T32511	T32509	punct	", ",DBD
R9244	T32512	T32513	compound	DBD,mrs
R9245	T32513	T32509	conj	mrs,DBD
R9246	T32514	T32513	punct	-,mrs
R9247	T32515	T32513	punct	", ",mrs
R9248	T32516	T32517	compound	DBD,N
R9249	T32517	T32513	conj	N,mrs
R9250	T32518	T32517	punct	-,N
R9251	T32519	T32517	cc	or,N
R9252	T32520	T32521	compound	DBD,C
R9253	T32521	T32517	conj	C,N
R9254	T32522	T32521	punct	-,C
R9255	T32523	T32504	auxpass	were,transfected
R9256	T32524	T32504	prep	with,transfected
R9257	T32525	T32526	nummod	0.1,μg
R9258	T32526	T32524	pobj	μg,with
R9259	T32527	T32526	prep	of,μg
R9260	T32528	T32529	compound	5xGAL4,pGL3
R9261	T32529	T32531	compound	pGL3,plasmid
R9262	T32530	T32529	punct	-,pGL3
R9263	T32531	T32527	pobj	plasmid,of
R9264	T32532	T32531	compound	reporter,plasmid
R9265	T32533	T32504	punct	.,transfected
R9266	T32535	T32536	det	The,activity
R9267	T32536	T32538	nsubjpass	activity,designated
R9268	T32537	T32536	compound	reporter,activity
R9269	T32539	T32536	prep	in,activity
R9270	T32540	T32541	det	the,presence
R9271	T32541	T32539	pobj	presence,in
R9272	T32542	T32541	prep	of,presence
R9273	T32543	T32544	det	the,vector
R9274	T32544	T32542	pobj	vector,of
R9275	T32545	T32544	compound	pcDNA3,vector
R9276	T32546	T32547	punct	(,pcDNA3
R9277	T32547	T32536	parataxis	pcDNA3,activity
R9278	T32548	T32547	punct	),pcDNA3
R9279	T32549	T32538	auxpass	was,designated
R9280	T32550	T32538	prep	as,designated
R9281	T32551	T32550	pobj	1,as
R9282	T32552	T32538	punct	.,designated
R9283	T32554	T32555	compound	Error,bars
R9284	T32555	T32556	nsubj	bars,represent
R9285	T32557	T32558	amod	standard,error
R9286	T32558	T32556	dobj	error,represent
R9287	T32559	T32558	prep	of,error
R9288	T32560	T32559	pobj	mean,of
R9289	T32561	T32556	punct	.,represent
R9290	T32563	T32564	punct	(,C
R9291	T32564	T32565	meta	C,co-transfected
R9292	T32566	T32564	punct	),C
R9293	T32567	T32568	compound	DBD,W404A
R9294	T32568	T32565	nsubjpass	W404A,co-transfected
R9295	T32569	T32568	punct	-,W404A
R9296	T32570	T32568	cc	and,W404A
R9297	T32571	T32572	compound	DBD,G453A
R9298	T32572	T32568	conj	G453A,W404A
R9299	T32573	T32572	punct	-,G453A
R9300	T32574	T32565	auxpass	were,co-transfected
R9301	T32575	T32565	prep	into,co-transfected
R9302	T32576	T32577	compound	HEK293T,cells
R9303	T32577	T32575	pobj	cells,into
R9304	T32578	T32565	prep	with,co-transfected
R9305	T32579	T32580	compound	5xGAL4,pGL3
R9306	T32580	T32582	compound	pGL3,plasmid
R9307	T32581	T32580	punct	-,pGL3
R9308	T32582	T32578	pobj	plasmid,with
R9309	T32583	T32582	compound	reporter,plasmid
R9310	T32584	T32565	punct	.,co-transfected
R9311	T32586	T32587	compound	Fold,repression
R9312	T32587	T32588	nsubjpass	repression,calculated
R9313	T32589	T32588	auxpass	was,calculated
R9314	T32590	T32588	prep	as,calculated
R9315	T32591	T32592	det	the,decrease
R9316	T32592	T32590	pobj	decrease,as
R9317	T32593	T32592	compound	fold,decrease
R9318	T32594	T32592	prep	in,decrease
R9319	T32595	T32596	compound	luciferase,activity
R9320	T32596	T32594	pobj	activity,in
R9321	T32597	T32592	prep	compared,decrease
R9322	T32598	T32597	prep	with,compared
R9323	T32599	T32600	compound	DBD,mrs
R9324	T32600	T32598	pobj	mrs,with
R9325	T32601	T32600	punct	-,mrs
R9326	T32602	T32588	punct	.,calculated
R9327	T32604	T32605	compound	Error,bars
R9328	T32605	T32606	nsubj	bars,represent
R9329	T32607	T32608	amod	standard,deviation
R9330	T32608	T32606	dobj	deviation,represent
R9331	T32609	T32606	punct	.,represent
R9332	T32611	T32612	punct	(,D
R9333	T32612	T32613	meta	D,transfected
R9334	T32614	T32612	punct	),D
R9335	T32615	T32616	amod	Various,amounts
R9336	T32616	T32613	nsubjpass	amounts,transfected
R9337	T32617	T32616	prep	of,amounts
R9338	T32618	T32619	compound	DBD,tail
R9339	T32619	T32617	pobj	tail,of
R9340	T32620	T32619	punct	-,tail
R9341	T32621	T32619	cc	or,tail
R9342	T32622	T32623	compound	DBD,SAM
R9343	T32623	T32619	conj	SAM,tail
R9344	T32624	T32623	punct	-,SAM
R9345	T32625	T32613	auxpass	were,transfected
R9346	T32626	T32613	prep	with,transfected
R9347	T32627	T32628	compound	5xGAL4,pGL3
R9348	T32628	T32630	compound	pGL3,plasmid
R9349	T32629	T32628	punct	-,pGL3
R9350	T32630	T32626	pobj	plasmid,with
R9351	T32631	T32630	compound	reporter,plasmid
R9352	T32632	T32613	punct	.,transfected
R9353	T32634	T32635	compound	Error,bars
R9354	T32635	T32636	nsubj	bars,represent
R9355	T32637	T32638	amod	standard,error
R9356	T32638	T32636	dobj	error,represent
R9357	T32639	T32638	prep	of,error
R9358	T32640	T32639	pobj	mean,of
R9359	T32641	T32636	punct	.,represent
R9360	T32643	T32644	punct	(,E
R9361	T32644	T32645	meta	E,co-transfected
R9362	T32646	T32644	punct	),E
R9363	T32647	T32645	nsubjpass	pcDNA3,co-transfected
R9364	T32648	T32647	cc	or,pcDNA3
R9365	T32649	T32650	compound	DBD,mrs
R9366	T32650	T32647	conj	mrs,pcDNA3
R9367	T32651	T32650	punct	-,mrs
R9368	T32652	T32653	punct	(,μg
R9369	T32653	T32650	parataxis	μg,mrs
R9370	T32654	T32653	nummod	5,μg
R9371	T32655	T32653	punct	),μg
R9372	T32656	T32645	auxpass	was,co-transfected
R9373	T32657	T32645	prep	into,co-transfected
R9374	T32658	T32659	compound	Y79,cells
R9375	T32659	T32657	pobj	cells,into
R9376	T32660	T32659	compound	retinoblastoma,cells
R9377	T32661	T32645	prep	with,co-transfected
R9378	T32662	T32663	nummod	0.5,μg
R9379	T32663	T32661	pobj	μg,with
R9380	T32664	T32663	prep	of,μg
R9381	T32665	T32666	compound	5xGAL4,pGL3
R9382	T32666	T32668	compound	pGL3,plasmid
R9383	T32667	T32666	punct	-,pGL3
R9384	T32668	T32664	pobj	plasmid,of
R9385	T32669	T32668	compound	reporter,plasmid
R9386	T32670	T32645	punct	.,co-transfected
R9387	T32672	T32673	det	The,activity
R9388	T32673	T32675	nsubjpass	activity,designated
R9389	T32674	T32673	compound	reporter,activity
R9390	T32676	T32673	prep	in,activity
R9391	T32677	T32678	det	the,presence
R9392	T32678	T32676	pobj	presence,in
R9393	T32679	T32678	prep	of,presence
R9394	T32680	T32679	pobj	pcDNA3,of
R9395	T32681	T32675	auxpass	was,designated
R9396	T32682	T32675	prep	as,designated
R9397	T32683	T32682	pobj	1,as
R9398	T32684	T32675	punct	.,designated
R9399	T32686	T32687	compound	Error,bars
R9400	T32687	T32688	nsubj	bars,represent
R9401	T32689	T32690	amod	standard,deviation
R9402	T32690	T32688	dobj	deviation,represent
R9403	T32691	T32688	punct	.,represent
R9404	T32693	T32694	nsubj	Asterisk,marks
R9405	T32695	T32696	advmod	statistically,significant
R9406	T32696	T32697	amod	significant,difference
R9407	T32697	T32694	dobj	difference,marks
R9408	T32698	T32699	punct	(,test
R9409	T32699	T32694	parataxis	test,marks
R9410	T32700	T32699	poss	Student,test
R9411	T32701	T32700	case	's,Student
R9412	T32702	T32699	compound	t,test
R9413	T32703	T32699	punct	: ,test
R9414	T32704	T32705	nsubj	p,0.03
R9415	T32705	T32699	ccomp	0.03,test
R9416	T32706	T32705	punct	<,0.03
R9417	T32707	T32699	punct	),test
R9418	T32708	T32694	punct	.,marks
R9419	T32710	T32711	punct	(,F
R9420	T32711	T32712	meta	F,Alignment
R9421	T32713	T32711	punct	),F
R9422	T32714	T32712	prep	of,Alignment
R9423	T32715	T32716	det	the,regions
R9424	T32716	T32714	pobj	regions,of
R9425	T32717	T32718	npadvmod	C,terminal
R9426	T32718	T32716	amod	terminal,regions
R9427	T32719	T32718	punct	-,terminal
R9428	T32720	T32716	prep	for,regions
R9429	T32721	T32722	nmod	mouse,proteins
R9430	T32722	T32720	pobj	proteins,for
R9431	T32723	T32721	punct	", ",mouse
R9432	T32724	T32721	conj	rat,mouse
R9433	T32725	T32724	punct	", ",rat
R9434	T32726	T32724	amod	human,rat
R9435	T32727	T32724	punct	", ",rat
R9436	T32728	T32724	conj	chick,rat
R9437	T32729	T32728	cc	and,chick
R9438	T32730	T32728	conj	zebrafish,chick
R9439	T32731	T32732	compound	mr,s
R9440	T32732	T32722	compound	s,proteins
R9441	T32733	T32732	punct	-,s
R9442	T32734	T32712	punct	.,Alignment
R9443	T32736	T32737	amod	Conserved,residues
R9444	T32737	T32740	nsubjpass	residues,shown
R9445	T32738	T32739	compound	amino,acid
R9446	T32739	T32737	compound	acid,residues
R9447	T32741	T32740	auxpass	are,shown
R9448	T32742	T32740	prep	with,shown
R9449	T32743	T32744	det	a,shadow
R9450	T32744	T32742	pobj	shadow,with
R9451	T32745	T32744	amod	dark,shadow
R9452	T32746	T32740	punct	.,shown

PMC:1435744 / 7013-31435 JSONTXT 4 Projects

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PMC:1435744 / 7013-31435 JSON TXT 4 Projects