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PMC:1435744 / 47128-48657 JSONTXT 4 Projects

Annnotations TAB TSV DIC JSON TextAE

Id Subject Object Predicate Lexical cue
T27912 0-3 IN denotes For
T27914 4-19 JJ denotes transcriptional
T27915 20-28 NN denotes analysis
T27916 29-31 IN denotes of
T27917 32-35 DT denotes the
T27919 36-39 CD denotes 1.2
T27921 39-40 HYPH denotes -
T27920 40-42 NN denotes kb
T27922 43-51 NN denotes promoter
T27918 52-58 NN denotes region
T27923 59-61 IN denotes of
T27924 62-64 NN denotes mr
T27926 64-65 HYPH denotes -
T27925 65-66 NN denotes s
T27927 66-68 , denotes ,
T27928 68-70 PRP denotes we
T27913 71-82 VBD denotes constructed
T27929 83-84 DT denotes a
T27931 85-93 NN denotes reporter
T27930 94-101 NN denotes plasmid
T27932 102-103 -LRB- denotes (
T27933 103-110 NN denotes pro1.2k
T27934 110-111 -RRB- denotes )
T27935 112-114 IN denotes by
T27936 115-125 VBG denotes subcloning
T27937 126-127 DT denotes a
T27939 128-131 CD denotes 1.2
T27941 131-132 HYPH denotes -
T27940 132-134 NN denotes kb
T27942 135-143 JJ denotes upstream
T27943 144-151 JJ denotes genomic
T27938 152-160 NN denotes fragment
T27944 161-163 IN denotes of
T27945 164-166 NN denotes mr
T27947 166-167 HYPH denotes -
T27946 167-168 NN denotes s
T27948 169-173 NN denotes gene
T27949 174-178 IN denotes into
T27950 179-180 DT denotes a
T27952 181-185 NN denotes pGL3
T27954 186-196 NN denotes luciferase
T27953 197-205 NN denotes reporter
T27951 206-213 NN denotes plasmid
T27955 214-215 -LRB- denotes (
T27956 215-222 NNP denotes Promega
T27957 222-223 -RRB- denotes )
T27958 223-224 . denotes .
T27959 224-386 sentence denotes The expression vectors were constructed by subcloning full-length Crx, Otx2, Nrl genes into a pMIK expression vector (a gift from Dr. K. Maruyama), respectively.
T27960 225-228 DT denotes The
T27962 229-239 NN denotes expression
T27961 240-247 NNS denotes vectors
T27964 248-252 VBD denotes were
T27963 253-264 VBN denotes constructed
T27965 265-267 IN denotes by
T27966 268-278 VBG denotes subcloning
T27967 279-283 JJ denotes full
T27969 283-284 HYPH denotes -
T27968 284-290 NN denotes length
T27971 291-294 NN denotes Crx
T27973 294-296 , denotes ,
T27974 296-300 NN denotes Otx2
T27975 300-302 , denotes ,
T27972 302-305 NN denotes Nrl
T27970 306-311 NNS denotes genes
T27976 312-316 IN denotes into
T27977 317-318 DT denotes a
T27979 319-323 NN denotes pMIK
T27980 324-334 NN denotes expression
T27978 335-341 NN denotes vector
T27981 342-343 -LRB- denotes (
T27983 343-344 DT denotes a
T27982 345-349 NN denotes gift
T27984 350-354 IN denotes from
T27985 355-358 NNP denotes Dr.
T27987 359-361 NNP denotes K.
T27986 362-370 NNP denotes Maruyama
T27988 370-371 -RRB- denotes )
T27989 371-373 , denotes ,
T27990 373-385 RB denotes respectively
T27991 385-386 . denotes .
T27992 386-503 sentence denotes For the "mut1259" vector, the Crx binding site at the -1259 bp position was mutated by replacing GGATTA with AGATCT.
T27993 387-390 IN denotes For
T27995 391-394 DT denotes the
T27997 395-396 `` denotes "
T27998 396-403 NN denotes mut1259
T27999 403-404 '' denotes "
T27996 405-411 NN denotes vector
T28000 411-413 , denotes ,
T28001 413-416 DT denotes the
T28003 417-420 NN denotes Crx
T28004 421-428 NN denotes binding
T28002 429-433 NN denotes site
T28005 434-436 IN denotes at
T28006 437-440 DT denotes the
T28008 441-442 SYM denotes -
T28009 442-446 CD denotes 1259
T28010 447-449 NN denotes bp
T28007 450-458 NN denotes position
T28011 459-462 VBD denotes was
T27994 463-470 VBN denotes mutated
T28012 471-473 IN denotes by
T28013 474-483 VBG denotes replacing
T28014 484-490 NN denotes GGATTA
T28015 491-495 IN denotes with
T28016 496-502 NN denotes AGATCT
T28017 502-503 . denotes .
T28018 503-618 sentence denotes For the "mut198" vector, the Crx binding site at the -198 bp position was mutated by replacing TAATCC with GAATTC.
T28019 504-507 IN denotes For
T28021 508-511 DT denotes the
T28023 512-513 `` denotes "
T28024 513-519 NN denotes mut198
T28025 519-520 '' denotes "
T28022 521-527 NN denotes vector
T28026 527-529 , denotes ,
T28027 529-532 DT denotes the
T28029 533-536 NN denotes Crx
T28030 537-544 NN denotes binding
T28028 545-549 NN denotes site
T28031 550-552 IN denotes at
T28032 553-556 DT denotes the
T28034 557-558 SYM denotes -
T28035 558-561 CD denotes 198
T28036 562-564 NN denotes bp
T28033 565-573 NN denotes position
T28037 574-577 VBD denotes was
T28020 578-585 VBN denotes mutated
T28038 586-588 IN denotes by
T28039 589-598 VBG denotes replacing
T28040 599-605 NN denotes TAATCC
T28041 606-610 IN denotes with
T28042 611-617 NN denotes GAATTC
T28043 617-618 . denotes .
T28044 618-731 sentence denotes For the "mut72" vector, the Crx binding site at the -72 bp position was mutated by replacing GGATTA with GAATTC.
T28045 619-622 IN denotes For
T28047 623-626 DT denotes the
T28049 627-628 `` denotes "
T28050 628-633 NN denotes mut72
T28051 633-634 '' denotes "
T28048 635-641 NN denotes vector
T28052 641-643 , denotes ,
T28053 643-646 DT denotes the
T28055 647-650 NN denotes Crx
T28056 651-658 NN denotes binding
T28054 659-663 NN denotes site
T28057 664-666 IN denotes at
T28058 667-670 DT denotes the
T28060 671-672 SYM denotes -
T28061 672-674 CD denotes 72
T28062 675-677 NN denotes bp
T28059 678-686 NN denotes position
T28063 687-690 VBD denotes was
T28046 691-698 VBN denotes mutated
T28064 699-701 IN denotes by
T28065 702-711 VBG denotes replacing
T28066 712-718 NN denotes GGATTA
T28067 719-723 IN denotes with
T28068 724-730 NN denotes GAATTC
T28069 730-731 . denotes .
T28070 731-802 sentence denotes For the "mut all" vector, all of three Crx binding sites were mutated.
T28071 732-735 IN denotes For
T28073 736-739 DT denotes the
T28075 740-741 `` denotes "
T28076 741-744 NN denotes mut
T28077 745-748 NN denotes all
T28078 748-749 '' denotes "
T28074 750-756 NN denotes vector
T28079 756-758 , denotes ,
T28080 758-761 DT denotes all
T28081 762-764 IN denotes of
T28082 765-770 CD denotes three
T28084 771-774 NN denotes Crx
T28085 775-782 NN denotes binding
T28083 783-788 NNS denotes sites
T28086 789-793 VBD denotes were
T28072 794-801 VBN denotes mutated
T28087 801-802 . denotes .
T28088 802-1068 sentence denotes 5xGAL4-pGL3 Control reporter plasmid, which contains five copies of the GAL4 DNA recognition sequence positioned immediately upstream of SV40 minimal promoter, was kindly gifted from Dr. T. Noguchi and used for analysis of the transcriptional activity of mr-s [50].
T28089 803-809 NN denotes 5xGAL4
T28091 809-810 HYPH denotes -
T28090 810-814 NN denotes pGL3
T28093 815-822 NN denotes Control
T28094 823-831 NN denotes reporter
T28092 832-839 NN denotes plasmid
T28096 839-841 , denotes ,
T28097 841-846 WDT denotes which
T28098 847-855 VBZ denotes contains
T28099 856-860 CD denotes five
T28100 861-867 NNS denotes copies
T28101 868-870 IN denotes of
T28102 871-874 DT denotes the
T28104 875-879 NN denotes GAL4
T28105 880-883 NN denotes DNA
T28106 884-895 NN denotes recognition
T28103 896-904 NN denotes sequence
T28107 905-915 VBN denotes positioned
T28108 916-927 RB denotes immediately
T28109 928-936 IN denotes upstream
T28110 937-939 IN denotes of
T28111 940-944 NN denotes SV40
T28113 945-952 JJ denotes minimal
T28112 953-961 NN denotes promoter
T28114 961-963 , denotes ,
T28115 963-966 VBD denotes was
T28116 967-973 RB denotes kindly
T28095 974-980 VBN denotes gifted
T28117 981-985 IN denotes from
T28118 986-989 NNP denotes Dr.
T28120 990-992 NNP denotes T.
T28119 993-1000 NNP denotes Noguchi
T28121 1001-1004 CC denotes and
T28122 1005-1009 VBN denotes used
T28123 1010-1013 IN denotes for
T28124 1014-1022 NN denotes analysis
T28125 1023-1025 IN denotes of
T28126 1026-1029 DT denotes the
T28128 1030-1045 JJ denotes transcriptional
T28127 1046-1054 NN denotes activity
T28129 1055-1057 IN denotes of
T28130 1058-1060 NN denotes mr
T28132 1060-1061 HYPH denotes -
T28131 1061-1062 NN denotes s
T28133 1063-1064 -LRB- denotes [
T28134 1064-1066 CD denotes 50
T28135 1066-1067 -RRB- denotes ]
T28136 1067-1068 . denotes .
T28137 1068-1204 sentence denotes To generate effector plasmids, various deletion fragments were produced by PCR reaction and subcloned into pGBKT7 vector, respectively.
T28138 1069-1071 TO denotes To
T28139 1072-1080 VB denotes generate
T28141 1081-1089 NN denotes effector
T28142 1090-1098 NNS denotes plasmids
T28143 1098-1100 , denotes ,
T28144 1100-1107 JJ denotes various
T28146 1108-1116 NN denotes deletion
T28145 1117-1126 NNS denotes fragments
T28147 1127-1131 VBD denotes were
T28140 1132-1140 VBN denotes produced
T28148 1141-1143 IN denotes by
T28149 1144-1147 NN denotes PCR
T28150 1148-1156 NN denotes reaction
T28151 1157-1160 CC denotes and
T28152 1161-1170 VBN denotes subcloned
T28153 1171-1175 IN denotes into
T28154 1176-1182 NN denotes pGBKT7
T28155 1183-1189 NN denotes vector
T28156 1189-1191 , denotes ,
T28157 1191-1203 RB denotes respectively
T28158 1203-1204 . denotes .
T28159 1204-1318 sentence denotes These fragments were digested with the sequence, which encodes GAL4 DNA binding domain, and inserted into pcDNA3.
T28160 1205-1210 DT denotes These
T28161 1211-1220 NNS denotes fragments
T28163 1221-1225 VBD denotes were
T28162 1226-1234 VBN denotes digested
T28164 1235-1239 IN denotes with
T28165 1240-1243 DT denotes the
T28166 1244-1252 NN denotes sequence
T28167 1252-1254 , denotes ,
T28168 1254-1259 WDT denotes which
T28169 1260-1267 VBZ denotes encodes
T28170 1268-1272 NN denotes GAL4
T28172 1273-1276 NN denotes DNA
T28173 1277-1284 NN denotes binding
T28171 1285-1291 NN denotes domain
T28174 1291-1293 , denotes ,
T28175 1293-1296 CC denotes and
T28176 1297-1305 VBN denotes inserted
T28177 1306-1310 IN denotes into
T28178 1311-1317 NN denotes pcDNA3
T28179 1317-1318 . denotes .
T28180 1318-1471 sentence denotes We transfected 0.1 μg of reporter plasmid DNA and 2 μg of the expression vector DNA per 6 cm dish into HEK293T cells using Fugene6 transfection reagent.
T28181 1319-1321 PRP denotes We
T28182 1322-1333 VBD denotes transfected
T28183 1334-1337 CD denotes 0.1
T28184 1338-1340 NN denotes μg
T28185 1341-1343 IN denotes of
T28186 1344-1352 NN denotes reporter
T28188 1353-1360 NN denotes plasmid
T28187 1361-1364 NN denotes DNA
T28189 1365-1368 CC denotes and
T28190 1369-1370 CD denotes 2
T28191 1371-1373 NN denotes μg
T28192 1374-1376 IN denotes of
T28193 1377-1380 DT denotes the
T28195 1381-1391 NN denotes expression
T28196 1392-1398 NN denotes vector
T28194 1399-1402 NN denotes DNA
T28197 1403-1406 IN denotes per
T28198 1407-1408 CD denotes 6
T28199 1409-1411 NN denotes cm
T28200 1412-1416 NN denotes dish
T28201 1417-1421 IN denotes into
T28202 1422-1429 NN denotes HEK293T
T28203 1430-1435 NNS denotes cells
T28204 1436-1441 VBG denotes using
T28205 1442-1449 NN denotes Fugene6
T28207 1450-1462 NN denotes transfection
T28206 1463-1470 NN denotes reagent
T28208 1470-1471 . denotes .
T28209 1471-1529 sentence denotes We analyzed luciferase activity 48 hr after transfection.
T28210 1472-1474 PRP denotes We
T28211 1475-1483 VBD denotes analyzed
T28212 1484-1494 NN denotes luciferase
T28213 1495-1503 NN denotes activity
T28214 1504-1506 CD denotes 48
T28215 1507-1509 NN denotes hr
T28216 1510-1515 IN denotes after
T28217 1516-1528 NN denotes transfection
T28218 1528-1529 . denotes .
R7871 T27912 T27913 prep For,constructed
R7872 T27914 T27915 amod transcriptional,analysis
R7873 T27915 T27912 pobj analysis,For
R7874 T27916 T27915 prep of,analysis
R7875 T27917 T27918 det the,region
R7876 T27918 T27916 pobj region,of
R7877 T27919 T27920 nummod 1.2,kb
R7878 T27920 T27918 compound kb,region
R7879 T27921 T27920 punct -,kb
R7880 T27922 T27918 compound promoter,region
R7881 T27923 T27918 prep of,region
R7882 T27924 T27925 compound mr,s
R7883 T27925 T27923 pobj s,of
R7884 T27926 T27925 punct -,s
R7885 T27927 T27913 punct ", ",constructed
R7886 T27928 T27913 nsubj we,constructed
R7887 T27929 T27930 det a,plasmid
R7888 T27930 T27913 dobj plasmid,constructed
R7889 T27931 T27930 compound reporter,plasmid
R7890 T27932 T27933 punct (,pro1.2k
R7891 T27933 T27930 parataxis pro1.2k,plasmid
R7892 T27934 T27933 punct ),pro1.2k
R7893 T27935 T27913 prep by,constructed
R7894 T27936 T27935 pcomp subcloning,by
R7895 T27937 T27938 det a,fragment
R7896 T27938 T27936 dobj fragment,subcloning
R7897 T27939 T27940 nummod 1.2,kb
R7898 T27940 T27938 nmod kb,fragment
R7899 T27941 T27940 punct -,kb
R7900 T27942 T27938 amod upstream,fragment
R7901 T27943 T27938 amod genomic,fragment
R7902 T27944 T27938 prep of,fragment
R7903 T27945 T27946 compound mr,s
R7904 T27946 T27948 compound s,gene
R7905 T27947 T27946 punct -,s
R7906 T27948 T27944 pobj gene,of
R7907 T27949 T27936 prep into,subcloning
R7908 T27950 T27951 det a,plasmid
R7909 T27951 T27949 pobj plasmid,into
R7910 T27952 T27953 compound pGL3,reporter
R7911 T27953 T27951 compound reporter,plasmid
R7912 T27954 T27953 compound luciferase,reporter
R7913 T27955 T27956 punct (,Promega
R7914 T27956 T27951 parataxis Promega,plasmid
R7915 T27957 T27956 punct ),Promega
R7916 T27958 T27913 punct .,constructed
R7917 T27960 T27961 det The,vectors
R7918 T27961 T27963 nsubjpass vectors,constructed
R7919 T27962 T27961 compound expression,vectors
R7920 T27964 T27963 auxpass were,constructed
R7921 T27965 T27963 prep by,constructed
R7922 T27966 T27965 pcomp subcloning,by
R7923 T27967 T27968 amod full,length
R7924 T27968 T27970 nmod length,genes
R7925 T27969 T27968 punct -,length
R7926 T27970 T27966 dobj genes,subcloning
R7927 T27971 T27972 nmod Crx,Nrl
R7928 T27972 T27970 compound Nrl,genes
R7929 T27973 T27972 punct ", ",Nrl
R7930 T27974 T27972 nmod Otx2,Nrl
R7931 T27975 T27972 punct ", ",Nrl
R7932 T27976 T27966 prep into,subcloning
R7933 T27977 T27978 det a,vector
R7934 T27978 T27976 pobj vector,into
R7935 T27979 T27978 compound pMIK,vector
R7936 T27980 T27978 compound expression,vector
R7937 T27981 T27982 punct (,gift
R7938 T27982 T27978 parataxis gift,vector
R7939 T27983 T27982 det a,gift
R7940 T27984 T27982 prep from,gift
R7941 T27985 T27986 compound Dr.,Maruyama
R7942 T27986 T27984 pobj Maruyama,from
R7943 T27987 T27986 compound K.,Maruyama
R7944 T27988 T27982 punct ),gift
R7945 T27989 T27966 punct ", ",subcloning
R7946 T27990 T27966 advmod respectively,subcloning
R7947 T27991 T27963 punct .,constructed
R7948 T27993 T27994 prep For,mutated
R7949 T27995 T27996 det the,vector
R7950 T27996 T27993 pobj vector,For
R7951 T27997 T27996 punct """",vector
R7952 T27998 T27996 nmod mut1259,vector
R7953 T27999 T27996 punct """",vector
R7954 T28000 T27994 punct ", ",mutated
R7955 T28001 T28002 det the,site
R7956 T28002 T27994 nsubjpass site,mutated
R7957 T28003 T28004 compound Crx,binding
R7958 T28004 T28002 compound binding,site
R7959 T28005 T28002 prep at,site
R7960 T28006 T28007 det the,position
R7961 T28007 T28005 pobj position,at
R7962 T28008 T28009 punct -,1259
R7963 T28009 T28010 nummod 1259,bp
R7964 T28010 T28007 compound bp,position
R7965 T28011 T27994 auxpass was,mutated
R7966 T28012 T27994 prep by,mutated
R7967 T28013 T28012 pcomp replacing,by
R7968 T28014 T28013 dobj GGATTA,replacing
R7969 T28015 T28013 prep with,replacing
R7970 T28016 T28015 pobj AGATCT,with
R7971 T28017 T27994 punct .,mutated
R7972 T28019 T28020 prep For,mutated
R7973 T28021 T28022 det the,vector
R7974 T28022 T28019 pobj vector,For
R7975 T28023 T28022 punct """",vector
R7976 T28024 T28022 nmod mut198,vector
R7977 T28025 T28022 punct """",vector
R7978 T28026 T28020 punct ", ",mutated
R7979 T28027 T28028 det the,site
R7980 T28028 T28020 nsubjpass site,mutated
R7981 T28029 T28030 compound Crx,binding
R7982 T28030 T28028 compound binding,site
R7983 T28031 T28028 prep at,site
R7984 T28032 T28033 det the,position
R7985 T28033 T28031 pobj position,at
R7986 T28034 T28035 punct -,198
R7987 T28035 T28036 nummod 198,bp
R7988 T28036 T28033 compound bp,position
R7989 T28037 T28020 auxpass was,mutated
R7990 T28038 T28020 prep by,mutated
R7991 T28039 T28038 pcomp replacing,by
R7992 T28040 T28039 dobj TAATCC,replacing
R7993 T28041 T28039 prep with,replacing
R7994 T28042 T28041 pobj GAATTC,with
R7995 T28043 T28020 punct .,mutated
R7996 T28045 T28046 prep For,mutated
R7997 T28047 T28048 det the,vector
R7998 T28048 T28045 pobj vector,For
R7999 T28049 T28048 punct """",vector
R8000 T28050 T28048 nmod mut72,vector
R8001 T28051 T28048 punct """",vector
R8002 T28052 T28046 punct ", ",mutated
R8003 T28053 T28054 det the,site
R8004 T28054 T28046 nsubjpass site,mutated
R8005 T28055 T28056 compound Crx,binding
R8006 T28056 T28054 compound binding,site
R8007 T28057 T28054 prep at,site
R8008 T28058 T28059 det the,position
R8009 T28059 T28057 pobj position,at
R8010 T28060 T28061 punct -,72
R8011 T28061 T28062 nummod 72,bp
R8012 T28062 T28059 compound bp,position
R8013 T28063 T28046 auxpass was,mutated
R8014 T28064 T28046 prep by,mutated
R8015 T28065 T28064 pcomp replacing,by
R8016 T28066 T28065 dobj GGATTA,replacing
R8017 T28067 T28065 prep with,replacing
R8018 T28068 T28067 pobj GAATTC,with
R8019 T28069 T28046 punct .,mutated
R8020 T28071 T28072 prep For,mutated
R8021 T28073 T28074 det the,vector
R8022 T28074 T28071 pobj vector,For
R8023 T28075 T28074 punct """",vector
R8024 T28076 T28077 nmod mut,all
R8025 T28077 T28074 nmod all,vector
R8026 T28078 T28074 punct """",vector
R8027 T28079 T28072 punct ", ",mutated
R8028 T28080 T28072 nsubjpass all,mutated
R8029 T28081 T28080 prep of,all
R8030 T28082 T28083 nummod three,sites
R8031 T28083 T28081 pobj sites,of
R8032 T28084 T28085 compound Crx,binding
R8033 T28085 T28083 compound binding,sites
R8034 T28086 T28072 auxpass were,mutated
R8035 T28087 T28072 punct .,mutated
R8036 T28089 T28090 compound 5xGAL4,pGL3
R8037 T28090 T28092 compound pGL3,plasmid
R8038 T28091 T28090 punct -,pGL3
R8039 T28092 T28095 nsubjpass plasmid,gifted
R8040 T28093 T28092 compound Control,plasmid
R8041 T28094 T28092 compound reporter,plasmid
R8042 T28096 T28092 punct ", ",plasmid
R8043 T28097 T28098 dep which,contains
R8044 T28098 T28092 relcl contains,plasmid
R8045 T28099 T28100 nummod five,copies
R8046 T28100 T28098 dobj copies,contains
R8047 T28101 T28100 prep of,copies
R8048 T28102 T28103 det the,sequence
R8049 T28103 T28101 pobj sequence,of
R8050 T28104 T28103 compound GAL4,sequence
R8051 T28105 T28106 compound DNA,recognition
R8052 T28106 T28103 compound recognition,sequence
R8053 T28107 T28100 acl positioned,copies
R8054 T28108 T28107 advmod immediately,positioned
R8055 T28109 T28108 advmod upstream,immediately
R8056 T28110 T28108 prep of,immediately
R8057 T28111 T28112 nmod SV40,promoter
R8058 T28112 T28110 pobj promoter,of
R8059 T28113 T28112 amod minimal,promoter
R8060 T28114 T28095 punct ", ",gifted
R8061 T28115 T28095 auxpass was,gifted
R8062 T28116 T28095 advmod kindly,gifted
R8063 T28117 T28095 agent from,gifted
R8064 T28118 T28119 compound Dr.,Noguchi
R8065 T28119 T28117 pobj Noguchi,from
R8066 T28120 T28119 compound T.,Noguchi
R8067 T28121 T28095 cc and,gifted
R8068 T28122 T28095 conj used,gifted
R8069 T28123 T28122 prep for,used
R8070 T28124 T28123 pobj analysis,for
R8071 T28125 T28124 prep of,analysis
R8072 T28126 T28127 det the,activity
R8073 T28127 T28125 pobj activity,of
R8074 T28128 T28127 amod transcriptional,activity
R8075 T28129 T28127 prep of,activity
R8076 T28130 T28131 compound mr,s
R8077 T28131 T28129 pobj s,of
R8078 T28132 T28131 punct -,s
R8079 T28133 T28134 punct [,50
R8080 T28134 T28122 parataxis 50,used
R8081 T28135 T28134 punct ],50
R8082 T28136 T28095 punct .,gifted
R8083 T28138 T28139 aux To,generate
R8084 T28139 T28140 advcl generate,produced
R8085 T28141 T28142 compound effector,plasmids
R8086 T28142 T28139 dobj plasmids,generate
R8087 T28143 T28140 punct ", ",produced
R8088 T28144 T28145 amod various,fragments
R8089 T28145 T28140 nsubj fragments,produced
R8090 T28146 T28145 compound deletion,fragments
R8091 T28147 T28140 aux were,produced
R8092 T28148 T28140 prep by,produced
R8093 T28149 T28150 compound PCR,reaction
R8094 T28150 T28148 pobj reaction,by
R8095 T28151 T28140 cc and,produced
R8096 T28152 T28140 conj subcloned,produced
R8097 T28153 T28152 prep into,subcloned
R8098 T28154 T28155 compound pGBKT7,vector
R8099 T28155 T28153 pobj vector,into
R8100 T28156 T28140 punct ", ",produced
R8101 T28157 T28140 advmod respectively,produced
R8102 T28158 T28140 punct .,produced
R8103 T28160 T28161 det These,fragments
R8104 T28161 T28162 nsubjpass fragments,digested
R8105 T28163 T28162 auxpass were,digested
R8106 T28164 T28162 prep with,digested
R8107 T28165 T28166 det the,sequence
R8108 T28166 T28164 pobj sequence,with
R8109 T28167 T28166 punct ", ",sequence
R8110 T28168 T28169 dep which,encodes
R8111 T28169 T28166 relcl encodes,sequence
R8112 T28170 T28171 compound GAL4,domain
R8113 T28171 T28169 dobj domain,encodes
R8114 T28172 T28173 compound DNA,binding
R8115 T28173 T28171 compound binding,domain
R8116 T28174 T28162 punct ", ",digested
R8117 T28175 T28162 cc and,digested
R8118 T28176 T28162 conj inserted,digested
R8119 T28177 T28176 prep into,inserted
R8120 T28178 T28177 pobj pcDNA3,into
R8121 T28179 T28162 punct .,digested
R8122 T28181 T28182 nsubj We,transfected
R8123 T28183 T28184 nummod 0.1,μg
R8124 T28184 T28182 dobj μg,transfected
R8125 T28185 T28184 prep of,μg
R8126 T28186 T28187 compound reporter,DNA
R8127 T28187 T28185 pobj DNA,of
R8128 T28188 T28187 compound plasmid,DNA
R8129 T28189 T28184 cc and,μg
R8130 T28190 T28191 nummod 2,μg
R8131 T28191 T28184 conj μg,μg
R8132 T28192 T28191 prep of,μg
R8133 T28193 T28194 det the,DNA
R8134 T28194 T28192 pobj DNA,of
R8135 T28195 T28196 compound expression,vector
R8136 T28196 T28194 compound vector,DNA
R8137 T28197 T28184 prep per,μg
R8138 T28198 T28199 nummod 6,cm
R8139 T28199 T28200 compound cm,dish
R8140 T28200 T28197 pobj dish,per
R8141 T28201 T28182 prep into,transfected
R8142 T28202 T28203 compound HEK293T,cells
R8143 T28203 T28201 pobj cells,into
R8144 T28204 T28182 advcl using,transfected
R8145 T28205 T28206 compound Fugene6,reagent
R8146 T28206 T28204 dobj reagent,using
R8147 T28207 T28206 compound transfection,reagent
R8148 T28208 T28182 punct .,transfected
R8149 T28210 T28211 nsubj We,analyzed
R8150 T28212 T28213 compound luciferase,activity
R8151 T28213 T28211 dobj activity,analyzed
R8152 T28214 T28215 nummod 48,hr
R8153 T28215 T28216 npadvmod hr,after
R8154 T28216 T28211 prep after,analyzed
R8155 T28217 T28216 pobj transfection,after
R8156 T28218 T28211 punct .,analyzed