| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T23802 |
0-4 |
NN |
denotes |
Cell |
| T23803 |
5-12 |
NN |
denotes |
culture |
| T23804 |
13-16 |
CC |
denotes |
and |
| T23805 |
17-29 |
NN |
denotes |
transfection |
| T23806 |
29-211 |
sentence |
denotes |
HEK293T cells were maintained at 37°C in Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% fetal bovine serum (Sigma), 100 IU/ml penicillin and 100 μg/ml streptomycin. |
| T23807 |
30-37 |
NN |
denotes |
HEK293T |
| T23808 |
38-43 |
NNS |
denotes |
cells |
| T23810 |
44-48 |
VBD |
denotes |
were |
| T23809 |
49-59 |
VBN |
denotes |
maintained |
| T23811 |
60-62 |
IN |
denotes |
at |
| T23812 |
63-65 |
CD |
denotes |
37 |
| T23813 |
65-67 |
NN |
denotes |
°C |
| T23814 |
68-70 |
IN |
denotes |
in |
| T23815 |
71-79 |
NNP |
denotes |
Dulbecco |
| T23817 |
79-81 |
POS |
denotes |
's |
| T23818 |
82-90 |
VBN |
denotes |
modified |
| T23819 |
91-96 |
NNP |
denotes |
Eagle |
| T23820 |
96-98 |
POS |
denotes |
's |
| T23816 |
99-105 |
NN |
denotes |
medium |
| T23821 |
106-107 |
-LRB- |
denotes |
( |
| T23822 |
107-111 |
NN |
denotes |
DMEM |
| T23823 |
111-112 |
-RRB- |
denotes |
) |
| T23824 |
113-125 |
VBN |
denotes |
supplemented |
| T23825 |
126-130 |
IN |
denotes |
with |
| T23826 |
131-133 |
CD |
denotes |
10 |
| T23827 |
133-134 |
NN |
denotes |
% |
| T23829 |
135-140 |
JJ |
denotes |
fetal |
| T23830 |
141-147 |
JJ |
denotes |
bovine |
| T23828 |
148-153 |
NN |
denotes |
serum |
| T23831 |
154-155 |
-LRB- |
denotes |
( |
| T23832 |
155-160 |
NNP |
denotes |
Sigma |
| T23833 |
160-161 |
-RRB- |
denotes |
) |
| T23834 |
161-163 |
, |
denotes |
, |
| T23835 |
163-166 |
CD |
denotes |
100 |
| T23836 |
167-169 |
NN |
denotes |
IU |
| T23838 |
169-170 |
SYM |
denotes |
/ |
| T23839 |
170-172 |
NN |
denotes |
ml |
| T23837 |
173-183 |
NN |
denotes |
penicillin |
| T23840 |
184-187 |
CC |
denotes |
and |
| T23841 |
188-191 |
CD |
denotes |
100 |
| T23842 |
192-194 |
NN |
denotes |
μg |
| T23844 |
194-195 |
SYM |
denotes |
/ |
| T23845 |
195-197 |
NN |
denotes |
ml |
| T23843 |
198-210 |
NN |
denotes |
streptomycin |
| T23846 |
210-211 |
. |
denotes |
. |
| T23847 |
211-339 |
sentence |
denotes |
Transient transfection of HEK293T cells was carried out using calcium phosphate method or Fugene6 transfection reagent (Roche). |
| T23848 |
212-221 |
JJ |
denotes |
Transient |
| T23849 |
222-234 |
NN |
denotes |
transfection |
| T23851 |
235-237 |
IN |
denotes |
of |
| T23852 |
238-245 |
NN |
denotes |
HEK293T |
| T23853 |
246-251 |
NNS |
denotes |
cells |
| T23854 |
252-255 |
VBD |
denotes |
was |
| T23850 |
256-263 |
VBN |
denotes |
carried |
| T23855 |
264-267 |
RP |
denotes |
out |
| T23856 |
268-273 |
VBG |
denotes |
using |
| T23857 |
274-281 |
NN |
denotes |
calcium |
| T23858 |
282-291 |
NN |
denotes |
phosphate |
| T23859 |
292-298 |
NN |
denotes |
method |
| T23860 |
299-301 |
CC |
denotes |
or |
| T23861 |
302-309 |
NN |
denotes |
Fugene6 |
| T23863 |
310-322 |
NN |
denotes |
transfection |
| T23862 |
323-330 |
NN |
denotes |
reagent |
| T23864 |
331-332 |
-LRB- |
denotes |
( |
| T23865 |
332-337 |
NNP |
denotes |
Roche |
| T23866 |
337-338 |
-RRB- |
denotes |
) |
| T23867 |
338-339 |
. |
denotes |
. |
| T23868 |
339-498 |
sentence |
denotes |
Y79 retinoblastoma cells were maintained in Iscove's modified Dulbecco's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 20% FBS. |
| T23869 |
340-343 |
NN |
denotes |
Y79 |
| T23871 |
344-358 |
NN |
denotes |
retinoblastoma |
| T23870 |
359-364 |
NNS |
denotes |
cells |
| T23873 |
365-369 |
VBD |
denotes |
were |
| T23872 |
370-380 |
VBN |
denotes |
maintained |
| T23874 |
381-383 |
IN |
denotes |
in |
| T23875 |
384-390 |
NNP |
denotes |
Iscove |
| T23877 |
390-392 |
POS |
denotes |
's |
| T23878 |
393-401 |
VBN |
denotes |
modified |
| T23879 |
402-410 |
NNP |
denotes |
Dulbecco |
| T23880 |
410-412 |
POS |
denotes |
's |
| T23876 |
413-419 |
NN |
denotes |
medium |
| T23881 |
420-424 |
IN |
denotes |
with |
| T23882 |
425-426 |
CD |
denotes |
4 |
| T23883 |
427-429 |
NN |
denotes |
mM |
| T23885 |
430-431 |
NN |
denotes |
L |
| T23886 |
431-432 |
HYPH |
denotes |
- |
| T23884 |
432-441 |
NN |
denotes |
glutamine |
| T23887 |
442-450 |
VBN |
denotes |
adjusted |
| T23888 |
451-453 |
TO |
denotes |
to |
| T23889 |
454-461 |
VB |
denotes |
contain |
| T23890 |
462-465 |
CD |
denotes |
1.5 |
| T23891 |
466-467 |
NN |
denotes |
g |
| T23893 |
467-468 |
SYM |
denotes |
/ |
| T23894 |
468-469 |
NN |
denotes |
L |
| T23895 |
470-476 |
NN |
denotes |
sodium |
| T23892 |
477-488 |
NN |
denotes |
bicarbonate |
| T23896 |
488-490 |
, |
denotes |
, |
| T23897 |
490-492 |
CD |
denotes |
20 |
| T23898 |
492-493 |
NN |
denotes |
% |
| T23899 |
494-497 |
NN |
denotes |
FBS |
| T23900 |
497-498 |
. |
denotes |
. |
| T23901 |
498-564 |
sentence |
denotes |
Transient transfection was carried out using TransIt LT1 (Mirus). |
| T23902 |
499-508 |
JJ |
denotes |
Transient |
| T23903 |
509-521 |
NN |
denotes |
transfection |
| T23905 |
522-525 |
VBD |
denotes |
was |
| T23904 |
526-533 |
VBN |
denotes |
carried |
| T23906 |
534-537 |
RP |
denotes |
out |
| T23907 |
538-543 |
VBG |
denotes |
using |
| T23908 |
544-551 |
NN |
denotes |
TransIt |
| T23909 |
552-555 |
NN |
denotes |
LT1 |
| T23910 |
556-557 |
-LRB- |
denotes |
( |
| T23911 |
557-562 |
NNP |
denotes |
Mirus |
| T23912 |
562-563 |
-RRB- |
denotes |
) |
| T23913 |
563-564 |
. |
denotes |
. |
| R6763 |
T23802 |
T23803 |
compound |
Cell,culture |
| R6764 |
T23804 |
T23803 |
cc |
and,culture |
| R6765 |
T23805 |
T23803 |
conj |
transfection,culture |
| R6766 |
T23807 |
T23808 |
compound |
HEK293T,cells |
| R6767 |
T23808 |
T23809 |
nsubjpass |
cells,maintained |
| R6768 |
T23810 |
T23809 |
auxpass |
were,maintained |
| R6769 |
T23811 |
T23809 |
prep |
at,maintained |
| R6770 |
T23812 |
T23813 |
nummod |
37,°C |
| R6771 |
T23813 |
T23811 |
pobj |
°C,at |
| R6772 |
T23814 |
T23809 |
prep |
in,maintained |
| R6773 |
T23815 |
T23816 |
poss |
Dulbecco,medium |
| R6774 |
T23816 |
T23814 |
pobj |
medium,in |
| R6775 |
T23817 |
T23815 |
case |
's,Dulbecco |
| R6776 |
T23818 |
T23816 |
amod |
modified,medium |
| R6777 |
T23819 |
T23816 |
poss |
Eagle,medium |
| R6778 |
T23820 |
T23819 |
case |
's,Eagle |
| R6779 |
T23821 |
T23816 |
punct |
(,medium |
| R6780 |
T23822 |
T23816 |
appos |
DMEM,medium |
| R6781 |
T23823 |
T23816 |
punct |
),medium |
| R6782 |
T23824 |
T23816 |
acl |
supplemented,medium |
| R6783 |
T23825 |
T23824 |
prep |
with,supplemented |
| R6784 |
T23826 |
T23827 |
nummod |
10,% |
| R6785 |
T23827 |
T23828 |
nmod |
%,serum |
| R6786 |
T23828 |
T23825 |
pobj |
serum,with |
| R6787 |
T23829 |
T23830 |
amod |
fetal,bovine |
| R6788 |
T23830 |
T23828 |
amod |
bovine,serum |
| R6789 |
T23831 |
T23832 |
punct |
(,Sigma |
| R6790 |
T23832 |
T23828 |
parataxis |
Sigma,serum |
| R6791 |
T23833 |
T23832 |
punct |
),Sigma |
| R6792 |
T23834 |
T23828 |
punct |
", ",serum |
| R6793 |
T23835 |
T23836 |
nummod |
100,IU |
| R6794 |
T23836 |
T23837 |
nmod |
IU,penicillin |
| R6795 |
T23837 |
T23828 |
conj |
penicillin,serum |
| R6796 |
T23838 |
T23839 |
punct |
/,ml |
| R6797 |
T23839 |
T23836 |
prep |
ml,IU |
| R6798 |
T23840 |
T23837 |
cc |
and,penicillin |
| R6799 |
T23841 |
T23842 |
nummod |
100,μg |
| R6800 |
T23842 |
T23843 |
nmod |
μg,streptomycin |
| R6801 |
T23843 |
T23837 |
conj |
streptomycin,penicillin |
| R6802 |
T23844 |
T23845 |
punct |
/,ml |
| R6803 |
T23845 |
T23842 |
prep |
ml,μg |
| R6804 |
T23846 |
T23809 |
punct |
.,maintained |
| R6805 |
T23848 |
T23849 |
amod |
Transient,transfection |
| R6806 |
T23849 |
T23850 |
nsubjpass |
transfection,carried |
| R6807 |
T23851 |
T23849 |
prep |
of,transfection |
| R6808 |
T23852 |
T23853 |
compound |
HEK293T,cells |
| R6809 |
T23853 |
T23851 |
pobj |
cells,of |
| R6810 |
T23854 |
T23850 |
auxpass |
was,carried |
| R6811 |
T23855 |
T23850 |
prt |
out,carried |
| R6812 |
T23856 |
T23850 |
advcl |
using,carried |
| R6813 |
T23857 |
T23858 |
compound |
calcium,phosphate |
| R6814 |
T23858 |
T23859 |
compound |
phosphate,method |
| R6815 |
T23859 |
T23856 |
dobj |
method,using |
| R6816 |
T23860 |
T23859 |
cc |
or,method |
| R6817 |
T23861 |
T23862 |
compound |
Fugene6,reagent |
| R6818 |
T23862 |
T23859 |
conj |
reagent,method |
| R6819 |
T23863 |
T23862 |
compound |
transfection,reagent |
| R6820 |
T23864 |
T23865 |
punct |
(,Roche |
| R6821 |
T23865 |
T23862 |
parataxis |
Roche,reagent |
| R6822 |
T23866 |
T23865 |
punct |
),Roche |
| R6823 |
T23867 |
T23850 |
punct |
.,carried |
| R6824 |
T23869 |
T23870 |
compound |
Y79,cells |
| R6825 |
T23870 |
T23872 |
nsubjpass |
cells,maintained |
| R6826 |
T23871 |
T23870 |
compound |
retinoblastoma,cells |
| R6827 |
T23873 |
T23872 |
auxpass |
were,maintained |
| R6828 |
T23874 |
T23872 |
prep |
in,maintained |
| R6829 |
T23875 |
T23876 |
poss |
Iscove,medium |
| R6830 |
T23876 |
T23874 |
pobj |
medium,in |
| R6831 |
T23877 |
T23875 |
case |
's,Iscove |
| R6832 |
T23878 |
T23876 |
amod |
modified,medium |
| R6833 |
T23879 |
T23876 |
poss |
Dulbecco,medium |
| R6834 |
T23880 |
T23879 |
case |
's,Dulbecco |
| R6835 |
T23881 |
T23876 |
prep |
with,medium |
| R6836 |
T23882 |
T23883 |
nummod |
4,mM |
| R6837 |
T23883 |
T23884 |
compound |
mM,glutamine |
| R6838 |
T23884 |
T23881 |
pobj |
glutamine,with |
| R6839 |
T23885 |
T23884 |
compound |
L,glutamine |
| R6840 |
T23886 |
T23884 |
punct |
-,glutamine |
| R6841 |
T23887 |
T23884 |
acl |
adjusted,glutamine |
| R6842 |
T23888 |
T23889 |
aux |
to,contain |
| R6843 |
T23889 |
T23887 |
advcl |
contain,adjusted |
| R6844 |
T23890 |
T23891 |
nummod |
1.5,g |
| R6845 |
T23891 |
T23892 |
nmod |
g,bicarbonate |
| R6846 |
T23892 |
T23889 |
dobj |
bicarbonate,contain |
| R6847 |
T23893 |
T23894 |
punct |
/,L |
| R6848 |
T23894 |
T23891 |
prep |
L,g |
| R6849 |
T23895 |
T23892 |
compound |
sodium,bicarbonate |
| R6850 |
T23896 |
T23892 |
punct |
", ",bicarbonate |
| R6851 |
T23897 |
T23898 |
nummod |
20,% |
| R6852 |
T23898 |
T23899 |
compound |
%,FBS |
| R6853 |
T23899 |
T23892 |
npadvmod |
FBS,bicarbonate |
| R6854 |
T23900 |
T23872 |
punct |
.,maintained |
| R6855 |
T23902 |
T23903 |
amod |
Transient,transfection |
| R6856 |
T23903 |
T23904 |
nsubjpass |
transfection,carried |
| R6857 |
T23905 |
T23904 |
auxpass |
was,carried |
| R6858 |
T23906 |
T23904 |
prt |
out,carried |
| R6859 |
T23907 |
T23904 |
advcl |
using,carried |
| R6860 |
T23908 |
T23909 |
compound |
TransIt,LT1 |
| R6861 |
T23909 |
T23907 |
dobj |
LT1,using |
| R6862 |
T23910 |
T23911 |
punct |
(,Mirus |
| R6863 |
T23911 |
T23909 |
parataxis |
Mirus,LT1 |
| R6864 |
T23912 |
T23911 |
punct |
),Mirus |
| R6865 |
T23913 |
T23904 |
punct |
.,carried |
