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PMC:1420314 / 17919-18513 JSONTXT 4 Projects

Annnotations TAB TSV DIC JSON TextAE

Id Subject Object Predicate Lexical cue
T11864 0-12 NN denotes Construction
T11865 13-15 IN denotes of
T11866 16-18 DT denotes an
T11868 19-25 NN denotes Adam11
T11869 26-30 NN denotes gene
T11870 30-31 HYPH denotes -
T11871 31-40 VBG denotes targeting
T11867 41-47 NN denotes vector
T11872 47-247 sentence denotes The 13.7-kb BamHI DNA fragment covering exons 2 – 20 of the Adam11 gene was amplified from C57BL/6 genomic DNA by the PCR using Expand Hi-Fidelity polymerase mix (Roche Diagnostics KK, Tokyo, Japan).
T11873 48-51 DT denotes The
T11875 52-56 CD denotes 13.7
T11877 56-57 HYPH denotes -
T11876 57-59 NN denotes kb
T11878 60-65 NN denotes BamHI
T11879 66-69 NN denotes DNA
T11874 70-78 NN denotes fragment
T11881 79-87 VBG denotes covering
T11882 88-93 NNS denotes exons
T11883 94-95 CD denotes 2
T11884 96-97 SYM denotes –
T11885 98-100 CD denotes 20
T11886 101-103 IN denotes of
T11887 104-107 DT denotes the
T11889 108-114 NN denotes Adam11
T11888 115-119 NN denotes gene
T11890 120-123 VBD denotes was
T11880 124-133 VBN denotes amplified
T11891 134-138 IN denotes from
T11892 139-144 NN denotes C57BL
T11894 144-145 HYPH denotes /
T11895 145-146 CD denotes 6
T11896 147-154 JJ denotes genomic
T11893 155-158 NN denotes DNA
T11897 159-161 IN denotes by
T11898 162-165 DT denotes the
T11899 166-169 NN denotes PCR
T11900 170-175 VBG denotes using
T11901 176-182 NN denotes Expand
T11903 183-185 NNP denotes Hi
T11905 185-186 HYPH denotes -
T11904 186-194 NNP denotes Fidelity
T11906 195-205 NN denotes polymerase
T11902 206-209 NN denotes mix
T11907 210-211 -LRB- denotes (
T11909 211-216 NNP denotes Roche
T11910 217-228 NNP denotes Diagnostics
T11908 229-231 NNP denotes KK
T11911 231-233 , denotes ,
T11912 233-238 NNP denotes Tokyo
T11913 238-240 , denotes ,
T11914 240-245 NNP denotes Japan
T11915 245-246 -RRB- denotes )
T11916 246-247 . denotes .
T11917 247-363 sentence denotes To generate a mutation in the mouse Adam11 gene, we inserted a PGKneo cassette into exons 5 – 7 of the Adam11 gene.
T11918 248-250 TO denotes To
T11919 251-259 VB denotes generate
T11921 260-261 DT denotes a
T11922 262-270 NN denotes mutation
T11923 271-273 IN denotes in
T11924 274-277 DT denotes the
T11926 278-283 NN denotes mouse
T11927 284-290 NN denotes Adam11
T11925 291-295 NN denotes gene
T11928 295-297 , denotes ,
T11929 297-299 PRP denotes we
T11920 300-308 VBD denotes inserted
T11930 309-310 DT denotes a
T11932 311-317 NN denotes PGKneo
T11931 318-326 NN denotes cassette
T11933 327-331 IN denotes into
T11934 332-337 NNS denotes exons
T11935 338-339 CD denotes 5
T11936 340-341 SYM denotes –
T11937 342-343 CD denotes 7
T11938 344-346 IN denotes of
T11939 347-350 DT denotes the
T11941 351-357 NN denotes Adam11
T11940 358-362 NN denotes gene
T11942 362-363 . denotes .
T11943 363-594 sentence denotes The final targeting construct consisted of a 10.1-kb genomic DNA fragment that was interrupted by the insertion of the PGKneo cassette and contained a MC1/TK cassette as a negative selection marker against random integration [39].
T11944 364-367 DT denotes The
T11946 368-373 JJ denotes final
T11947 374-383 NN denotes targeting
T11945 384-393 NN denotes construct
T11948 394-403 VBD denotes consisted
T11949 404-406 IN denotes of
T11950 407-408 DT denotes a
T11952 409-413 CD denotes 10.1
T11954 413-414 HYPH denotes -
T11953 414-416 NN denotes kb
T11955 417-424 JJ denotes genomic
T11956 425-428 NN denotes DNA
T11951 429-437 NN denotes fragment
T11957 438-442 WDT denotes that
T11959 443-446 VBD denotes was
T11958 447-458 VBN denotes interrupted
T11960 459-461 IN denotes by
T11961 462-465 DT denotes the
T11962 466-475 NN denotes insertion
T11963 476-478 IN denotes of
T11964 479-482 DT denotes the
T11966 483-489 NN denotes PGKneo
T11965 490-498 NN denotes cassette
T11967 499-502 CC denotes and
T11968 503-512 VBD denotes contained
T11969 513-514 DT denotes a
T11971 515-518 NN denotes MC1
T11973 518-519 HYPH denotes /
T11972 519-521 NN denotes TK
T11970 522-530 NN denotes cassette
T11974 531-533 IN denotes as
T11975 534-535 DT denotes a
T11977 536-544 JJ denotes negative
T11978 545-554 NN denotes selection
T11976 555-561 NN denotes marker
T11979 562-569 IN denotes against
T11980 570-576 JJ denotes random
T11981 577-588 NN denotes integration
T11982 589-590 -LRB- denotes [
T11983 590-592 CD denotes 39
T11984 592-593 -RRB- denotes ]
T11985 593-594 . denotes .
R3521 T11865 T11864 prep of,Construction
R3522 T11866 T11867 det an,vector
R3523 T11867 T11865 pobj vector,of
R3524 T11868 T11867 nmod Adam11,vector
R3525 T11869 T11867 nmod gene,vector
R3526 T11870 T11869 punct -,gene
R3527 T11871 T11869 amod targeting,gene
R3528 T11873 T11874 det The,fragment
R3529 T11874 T11880 nsubjpass fragment,amplified
R3530 T11875 T11876 nummod 13.7,kb
R3531 T11876 T11874 compound kb,fragment
R3532 T11877 T11876 punct -,kb
R3533 T11878 T11874 compound BamHI,fragment
R3534 T11879 T11874 compound DNA,fragment
R3535 T11881 T11874 acl covering,fragment
R3536 T11882 T11883 nmod exons,2
R3537 T11883 T11881 dobj 2,covering
R3538 T11884 T11885 punct –,20
R3539 T11885 T11883 prep 20,2
R3540 T11886 T11883 prep of,2
R3541 T11887 T11888 det the,gene
R3542 T11888 T11886 pobj gene,of
R3543 T11889 T11888 compound Adam11,gene
R3544 T11890 T11880 auxpass was,amplified
R3545 T11891 T11880 prep from,amplified
R3546 T11892 T11893 nmod C57BL,DNA
R3547 T11893 T11891 pobj DNA,from
R3548 T11894 T11892 punct /,C57BL
R3549 T11895 T11892 nummod 6,C57BL
R3550 T11896 T11893 amod genomic,DNA
R3551 T11897 T11880 prep by,amplified
R3552 T11898 T11899 det the,PCR
R3553 T11899 T11897 pobj PCR,by
R3554 T11900 T11899 acl using,PCR
R3555 T11901 T11902 compound Expand,mix
R3556 T11902 T11900 dobj mix,using
R3557 T11903 T11904 compound Hi,Fidelity
R3558 T11904 T11902 compound Fidelity,mix
R3559 T11905 T11904 punct -,Fidelity
R3560 T11906 T11902 compound polymerase,mix
R3561 T11907 T11908 punct (,KK
R3562 T11908 T11902 parataxis KK,mix
R3563 T11909 T11908 compound Roche,KK
R3564 T11910 T11908 compound Diagnostics,KK
R3565 T11911 T11908 punct ", ",KK
R3566 T11912 T11908 npadvmod Tokyo,KK
R3567 T11913 T11908 punct ", ",KK
R3568 T11914 T11908 npadvmod Japan,KK
R3569 T11915 T11908 punct ),KK
R3570 T11916 T11880 punct .,amplified
R3571 T11918 T11919 aux To,generate
R3572 T11919 T11920 advcl generate,inserted
R3573 T11921 T11922 det a,mutation
R3574 T11922 T11919 dobj mutation,generate
R3575 T11923 T11919 prep in,generate
R3576 T11924 T11925 det the,gene
R3577 T11925 T11923 pobj gene,in
R3578 T11926 T11925 compound mouse,gene
R3579 T11927 T11925 compound Adam11,gene
R3580 T11928 T11920 punct ", ",inserted
R3581 T11929 T11920 nsubj we,inserted
R3582 T11930 T11931 det a,cassette
R3583 T11931 T11920 dobj cassette,inserted
R3584 T11932 T11931 compound PGKneo,cassette
R3585 T11933 T11920 prep into,inserted
R3586 T11934 T11935 nmod exons,5
R3587 T11935 T11933 pobj 5,into
R3588 T11936 T11937 punct –,7
R3589 T11937 T11935 prep 7,5
R3590 T11938 T11935 prep of,5
R3591 T11939 T11940 det the,gene
R3592 T11940 T11938 pobj gene,of
R3593 T11941 T11940 compound Adam11,gene
R3594 T11942 T11920 punct .,inserted
R3595 T11944 T11945 det The,construct
R3596 T11945 T11948 nsubj construct,consisted
R3597 T11946 T11945 amod final,construct
R3598 T11947 T11945 compound targeting,construct
R3599 T11949 T11948 prep of,consisted
R3600 T11950 T11951 det a,fragment
R3601 T11951 T11949 pobj fragment,of
R3602 T11952 T11953 nummod 10.1,kb
R3603 T11953 T11951 nmod kb,fragment
R3604 T11954 T11953 punct -,kb
R3605 T11955 T11951 amod genomic,fragment
R3606 T11956 T11951 compound DNA,fragment
R3607 T11957 T11958 dep that,interrupted
R3608 T11958 T11951 relcl interrupted,fragment
R3609 T11959 T11958 auxpass was,interrupted
R3610 T11960 T11958 agent by,interrupted
R3611 T11961 T11962 det the,insertion
R3612 T11962 T11960 pobj insertion,by
R3613 T11963 T11962 prep of,insertion
R3614 T11964 T11965 det the,cassette
R3615 T11965 T11963 pobj cassette,of
R3616 T11966 T11965 compound PGKneo,cassette
R3617 T11967 T11958 cc and,interrupted
R3618 T11968 T11958 conj contained,interrupted
R3619 T11969 T11970 det a,cassette
R3620 T11970 T11968 dobj cassette,contained
R3621 T11971 T11972 compound MC1,TK
R3622 T11972 T11970 compound TK,cassette
R3623 T11973 T11972 punct /,TK
R3624 T11974 T11968 prep as,contained
R3625 T11975 T11976 det a,marker
R3626 T11976 T11974 pobj marker,as
R3627 T11977 T11978 amod negative,selection
R3628 T11978 T11976 compound selection,marker
R3629 T11979 T11976 prep against,marker
R3630 T11980 T11981 amod random,integration
R3631 T11981 T11979 pobj integration,against
R3632 T11982 T11983 punct [,39
R3633 T11983 T11968 parataxis 39,contained
R3634 T11984 T11983 punct ],39
R3635 T11985 T11948 punct .,consisted