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PMC:1420314 / 17910-25445 JSONTXT 4 Projects

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Id Subject Object Predicate Lexical cue
T11864 9-21 NN denotes Construction
T11865 22-24 IN denotes of
T11866 25-27 DT denotes an
T11868 28-34 NN denotes Adam11
T11869 35-39 NN denotes gene
T11870 39-40 HYPH denotes -
T11871 40-49 VBG denotes targeting
T11867 50-56 NN denotes vector
T11872 56-256 sentence denotes The 13.7-kb BamHI DNA fragment covering exons 2 – 20 of the Adam11 gene was amplified from C57BL/6 genomic DNA by the PCR using Expand Hi-Fidelity polymerase mix (Roche Diagnostics KK, Tokyo, Japan).
T11873 57-60 DT denotes The
T11875 61-65 CD denotes 13.7
T11877 65-66 HYPH denotes -
T11876 66-68 NN denotes kb
T11878 69-74 NN denotes BamHI
T11879 75-78 NN denotes DNA
T11874 79-87 NN denotes fragment
T11881 88-96 VBG denotes covering
T11882 97-102 NNS denotes exons
T11883 103-104 CD denotes 2
T11884 105-106 SYM denotes –
T11885 107-109 CD denotes 20
T11886 110-112 IN denotes of
T11887 113-116 DT denotes the
T11889 117-123 NN denotes Adam11
T11888 124-128 NN denotes gene
T11890 129-132 VBD denotes was
T11880 133-142 VBN denotes amplified
T11891 143-147 IN denotes from
T11892 148-153 NN denotes C57BL
T11894 153-154 HYPH denotes /
T11895 154-155 CD denotes 6
T11896 156-163 JJ denotes genomic
T11893 164-167 NN denotes DNA
T11897 168-170 IN denotes by
T11898 171-174 DT denotes the
T11899 175-178 NN denotes PCR
T11900 179-184 VBG denotes using
T11901 185-191 NN denotes Expand
T11903 192-194 NNP denotes Hi
T11905 194-195 HYPH denotes -
T11904 195-203 NNP denotes Fidelity
T11906 204-214 NN denotes polymerase
T11902 215-218 NN denotes mix
T11907 219-220 -LRB- denotes (
T11909 220-225 NNP denotes Roche
T11910 226-237 NNP denotes Diagnostics
T11908 238-240 NNP denotes KK
T11911 240-242 , denotes ,
T11912 242-247 NNP denotes Tokyo
T11913 247-249 , denotes ,
T11914 249-254 NNP denotes Japan
T11915 254-255 -RRB- denotes )
T11916 255-256 . denotes .
T11917 256-372 sentence denotes To generate a mutation in the mouse Adam11 gene, we inserted a PGKneo cassette into exons 5 – 7 of the Adam11 gene.
T11918 257-259 TO denotes To
T11919 260-268 VB denotes generate
T11921 269-270 DT denotes a
T11922 271-279 NN denotes mutation
T11923 280-282 IN denotes in
T11924 283-286 DT denotes the
T11926 287-292 NN denotes mouse
T11927 293-299 NN denotes Adam11
T11925 300-304 NN denotes gene
T11928 304-306 , denotes ,
T11929 306-308 PRP denotes we
T11920 309-317 VBD denotes inserted
T11930 318-319 DT denotes a
T11932 320-326 NN denotes PGKneo
T11931 327-335 NN denotes cassette
T11933 336-340 IN denotes into
T11934 341-346 NNS denotes exons
T11935 347-348 CD denotes 5
T11936 349-350 SYM denotes –
T11937 351-352 CD denotes 7
T11938 353-355 IN denotes of
T11939 356-359 DT denotes the
T11941 360-366 NN denotes Adam11
T11940 367-371 NN denotes gene
T11942 371-372 . denotes .
T11943 372-603 sentence denotes The final targeting construct consisted of a 10.1-kb genomic DNA fragment that was interrupted by the insertion of the PGKneo cassette and contained a MC1/TK cassette as a negative selection marker against random integration [39].
T11944 373-376 DT denotes The
T11946 377-382 JJ denotes final
T11947 383-392 NN denotes targeting
T11945 393-402 NN denotes construct
T11948 403-412 VBD denotes consisted
T11949 413-415 IN denotes of
T11950 416-417 DT denotes a
T11952 418-422 CD denotes 10.1
T11954 422-423 HYPH denotes -
T11953 423-425 NN denotes kb
T11955 426-433 JJ denotes genomic
T11956 434-437 NN denotes DNA
T11951 438-446 NN denotes fragment
T11957 447-451 WDT denotes that
T11959 452-455 VBD denotes was
T11958 456-467 VBN denotes interrupted
T11960 468-470 IN denotes by
T11961 471-474 DT denotes the
T11962 475-484 NN denotes insertion
T11963 485-487 IN denotes of
T11964 488-491 DT denotes the
T11966 492-498 NN denotes PGKneo
T11965 499-507 NN denotes cassette
T11967 508-511 CC denotes and
T11968 512-521 VBD denotes contained
T11969 522-523 DT denotes a
T11971 524-527 NN denotes MC1
T11973 527-528 HYPH denotes /
T11972 528-530 NN denotes TK
T11970 531-539 NN denotes cassette
T11974 540-542 IN denotes as
T11975 543-544 DT denotes a
T11977 545-553 JJ denotes negative
T11978 554-563 NN denotes selection
T11976 564-570 NN denotes marker
T11979 571-578 IN denotes against
T11980 579-585 JJ denotes random
T11981 586-597 NN denotes integration
T11982 598-599 -LRB- denotes [
T11983 599-601 CD denotes 39
T11984 601-602 -RRB- denotes ]
T11985 602-603 . denotes .
T12309 605-615 NN denotes Generation
T12310 616-618 IN denotes of
T12311 619-625 NN denotes Adam11
T12312 626-635 JJ denotes deficient
T12313 636-640 NNS denotes mice
T12314 640-732 sentence denotes The linearised targeting vector was electroporated into TT2 embryonic stem (ES) cells [40].
T12315 641-644 DT denotes The
T12317 645-655 VBN denotes linearised
T12318 656-665 NN denotes targeting
T12316 666-672 NN denotes vector
T12320 673-676 VBD denotes was
T12319 677-691 VBN denotes electroporated
T12321 692-696 IN denotes into
T12322 697-700 NN denotes TT2
T12324 701-710 JJ denotes embryonic
T12325 711-715 NN denotes stem
T12326 716-717 -LRB- denotes (
T12327 717-719 NN denotes ES
T12328 719-720 -RRB- denotes )
T12323 721-726 NNS denotes cells
T12329 727-728 -LRB- denotes [
T12330 728-730 CD denotes 40
T12331 730-731 -RRB- denotes ]
T12332 731-732 . denotes .
T12333 732-946 sentence denotes Homologous recombinants were selected by PCR using the following primers, AGN1: 5'-TCGTGCTTTACGGTATCGCCGCTCCCGATT-3' in the PGKneo cassette, and SGN033: 5'-GGACCCGGAAGACATTTGCACGGT-3' outside the targeting vector.
T12334 733-743 JJ denotes Homologous
T12335 744-756 NNS denotes recombinants
T12337 757-761 VBD denotes were
T12336 762-770 VBN denotes selected
T12338 771-773 IN denotes by
T12339 774-777 NN denotes PCR
T12340 778-783 VBG denotes using
T12341 784-787 DT denotes the
T12343 788-797 VBG denotes following
T12342 798-805 NNS denotes primers
T12344 805-807 , denotes ,
T12345 807-811 NN denotes AGN1
T12346 811-813 : denotes :
T12347 813-814 CD denotes 5
T12348 814-815 SYM denotes '
T12349 815-816 HYPH denotes -
T12350 816-846 NN denotes TCGTGCTTTACGGTATCGCCGCTCCCGATT
T12351 846-847 HYPH denotes -
T12352 847-848 CD denotes 3
T12353 848-849 SYM denotes '
T12354 850-852 IN denotes in
T12355 853-856 DT denotes the
T12357 857-863 NN denotes PGKneo
T12356 864-872 NN denotes cassette
T12358 872-874 , denotes ,
T12359 874-877 CC denotes and
T12360 878-884 NN denotes SGN033
T12361 884-886 : denotes :
T12362 886-887 CD denotes 5
T12363 887-888 SYM denotes '
T12364 888-889 HYPH denotes -
T12365 889-913 NN denotes GGACCCGGAAGACATTTGCACGGT
T12366 913-914 HYPH denotes -
T12367 914-915 CD denotes 3
T12368 915-916 SYM denotes '
T12369 917-924 IN denotes outside
T12370 925-928 DT denotes the
T12372 929-938 NN denotes targeting
T12371 939-945 NN denotes vector
T12373 945-946 . denotes .
T12374 946-1078 sentence denotes Homologous recombined DNA was efficiently amplified by 35 cycles of the following amplification steps (94°C-30 sec and 68°C-5 min).
T12375 947-957 JJ denotes Homologous
T12377 958-968 VBN denotes recombined
T12376 969-972 NN denotes DNA
T12379 973-976 VBD denotes was
T12380 977-988 RB denotes efficiently
T12378 989-998 VBN denotes amplified
T12381 999-1001 IN denotes by
T12382 1002-1004 CD denotes 35
T12383 1005-1011 NNS denotes cycles
T12384 1012-1014 IN denotes of
T12385 1015-1018 DT denotes the
T12387 1019-1028 VBG denotes following
T12388 1029-1042 NN denotes amplification
T12386 1043-1048 NNS denotes steps
T12389 1049-1050 -LRB- denotes (
T12390 1050-1052 CD denotes 94
T12391 1052-1054 NN denotes °C
T12392 1054-1055 SYM denotes -
T12393 1055-1057 CD denotes 30
T12394 1058-1061 NN denotes sec
T12395 1062-1065 CC denotes and
T12396 1066-1068 CD denotes 68
T12397 1068-1070 NN denotes °C
T12398 1070-1071 SYM denotes -
T12399 1071-1072 CD denotes 5
T12400 1073-1076 NN denotes min
T12401 1076-1077 -RRB- denotes )
T12402 1077-1078 . denotes .
T12403 1078-1177 sentence denotes Correctly targeted ES clones were injected into fertilised ICR mouse eggs at the eight-cell stage.
T12404 1079-1088 RB denotes Correctly
T12405 1089-1097 VBN denotes targeted
T12407 1098-1100 NN denotes ES
T12406 1101-1107 NNS denotes clones
T12409 1108-1112 VBD denotes were
T12408 1113-1121 VBN denotes injected
T12410 1122-1126 IN denotes into
T12411 1127-1137 VBN denotes fertilised
T12413 1138-1141 NN denotes ICR
T12414 1142-1147 NN denotes mouse
T12412 1148-1152 NNS denotes eggs
T12415 1153-1155 IN denotes at
T12416 1156-1159 DT denotes the
T12418 1160-1165 CD denotes eight
T12420 1165-1166 HYPH denotes -
T12419 1166-1170 NN denotes cell
T12417 1171-1176 NN denotes stage
T12421 1176-1177 . denotes .
T12422 1177-1382 sentence denotes The resulting male chimeras were mated with C57BL/6NCrj females (Charles River Japan, Tokyo, Japan), and resulting heterozygous (+/-) male and female mice were interbred to generate homozygous (-/-) mice.
T12423 1178-1181 DT denotes The
T12425 1182-1191 VBG denotes resulting
T12426 1192-1196 JJ denotes male
T12424 1197-1205 NNS denotes chimeras
T12428 1206-1210 VBD denotes were
T12427 1211-1216 VBN denotes mated
T12429 1217-1221 IN denotes with
T12430 1222-1227 NN denotes C57BL
T12432 1227-1228 HYPH denotes /
T12431 1228-1233 NN denotes 6NCrj
T12433 1234-1241 NNS denotes females
T12434 1242-1243 -LRB- denotes (
T12436 1243-1250 NNP denotes Charles
T12437 1251-1256 NNP denotes River
T12435 1257-1262 NNP denotes Japan
T12438 1262-1264 , denotes ,
T12439 1264-1269 NNP denotes Tokyo
T12440 1269-1271 , denotes ,
T12441 1271-1276 NNP denotes Japan
T12442 1276-1277 -RRB- denotes )
T12443 1277-1279 , denotes ,
T12444 1279-1282 CC denotes and
T12445 1283-1292 VBG denotes resulting
T12447 1293-1305 JJ denotes heterozygous
T12448 1306-1307 -LRB- denotes (
T12449 1307-1308 SYM denotes +
T12451 1308-1309 HYPH denotes /
T12450 1309-1310 SYM denotes -
T12452 1310-1311 -RRB- denotes )
T12453 1312-1316 JJ denotes male
T12454 1317-1320 CC denotes and
T12455 1321-1327 JJ denotes female
T12446 1328-1332 NNS denotes mice
T12457 1333-1337 VBD denotes were
T12456 1338-1347 VBN denotes interbred
T12458 1348-1350 TO denotes to
T12459 1351-1359 VB denotes generate
T12460 1360-1370 JJ denotes homozygous
T12462 1371-1372 -LRB- denotes (
T12463 1372-1373 SYM denotes -
T12465 1373-1374 HYPH denotes /
T12464 1374-1375 SYM denotes -
T12466 1375-1376 -RRB- denotes )
T12461 1377-1381 NNS denotes mice
T12467 1381-1382 . denotes .
T12588 1384-1392 NNP denotes Southern
T12589 1393-1397 NN denotes blot
T12590 1398-1406 NN denotes analysis
T12591 1406-1503 sentence denotes Mouse genomic DNA used for Southern blot analysis was extracted from the liver of each genotype.
T12592 1407-1412 NN denotes Mouse
T12594 1413-1420 JJ denotes genomic
T12593 1421-1424 NN denotes DNA
T12596 1425-1429 VBN denotes used
T12597 1430-1433 IN denotes for
T12598 1434-1442 NNP denotes Southern
T12599 1443-1447 NN denotes blot
T12600 1448-1456 NN denotes analysis
T12601 1457-1460 VBD denotes was
T12595 1461-1470 VBN denotes extracted
T12602 1471-1475 IN denotes from
T12603 1476-1479 DT denotes the
T12604 1480-1485 NN denotes liver
T12605 1486-1488 IN denotes of
T12606 1489-1493 DT denotes each
T12607 1494-1502 NN denotes genotype
T12608 1502-1503 . denotes .
T12609 1503-1648 sentence denotes BamHI-digested genomic DNA was probed with the [32P]-labelled BE2K probe, which was a 2.0-kb BamHI – EcoRI genomic fragment located in intron 2.
T12610 1504-1509 NN denotes BamHI
T12612 1509-1510 HYPH denotes -
T12611 1510-1518 VBN denotes digested
T12614 1519-1526 JJ denotes genomic
T12613 1527-1530 NN denotes DNA
T12616 1531-1534 VBD denotes was
T12615 1535-1541 VBN denotes probed
T12617 1542-1546 IN denotes with
T12618 1547-1550 DT denotes the
T12620 1551-1552 -LRB- denotes [
T12621 1552-1555 NN denotes 32P
T12623 1555-1556 -RRB- denotes ]
T12624 1556-1557 HYPH denotes -
T12622 1557-1565 VBN denotes labelled
T12625 1566-1570 NN denotes BE2K
T12619 1571-1576 NN denotes probe
T12626 1576-1578 , denotes ,
T12627 1578-1583 WDT denotes which
T12628 1584-1587 VBD denotes was
T12629 1588-1589 DT denotes a
T12631 1590-1593 CD denotes 2.0
T12633 1593-1594 HYPH denotes -
T12632 1594-1596 NN denotes kb
T12634 1597-1602 NN denotes BamHI
T12636 1603-1604 HYPH denotes –
T12635 1605-1610 NN denotes EcoRI
T12637 1611-1618 JJ denotes genomic
T12630 1619-1627 NN denotes fragment
T12638 1628-1635 VBN denotes located
T12639 1636-1638 IN denotes in
T12640 1639-1645 NN denotes intron
T12641 1646-1647 CD denotes 2
T12642 1647-1648 . denotes .
T13011 1650-1657 NNP denotes Western
T13012 1658-1662 NN denotes blot
T13013 1663-1671 NN denotes analysis
T13014 1671-1759 sentence denotes The absence of ADAM11 protein in the (-/-) mice was confirmed by Western blot analysis.
T13015 1672-1675 DT denotes The
T13016 1676-1683 NN denotes absence
T13018 1684-1686 IN denotes of
T13019 1687-1693 NN denotes ADAM11
T13020 1694-1701 NN denotes protein
T13021 1702-1704 IN denotes in
T13022 1705-1708 DT denotes the
T13024 1709-1710 -LRB- denotes (
T13025 1710-1711 SYM denotes -
T13027 1711-1712 HYPH denotes /
T13026 1712-1713 SYM denotes -
T13028 1713-1714 -RRB- denotes )
T13023 1715-1719 NNS denotes mice
T13029 1720-1723 VBD denotes was
T13017 1724-1733 VBN denotes confirmed
T13030 1734-1736 IN denotes by
T13031 1737-1744 NNP denotes Western
T13032 1745-1749 NN denotes blot
T13033 1750-1758 NN denotes analysis
T13034 1758-1759 . denotes .
T13035 1759-1979 sentence denotes To be brief, the cerebellum was isolated from a six month-old mouse of each genotype and homogenised in cell lysis buffer (50 mM Tris-HCl, pH 7.5, 100 mM NaCl, 1% NP-40, protease inhibitor cocktail [Roche Diagnostics]).
T13036 1760-1762 TO denotes To
T13037 1763-1765 VB denotes be
T13039 1766-1771 JJ denotes brief
T13040 1771-1773 , denotes ,
T13041 1773-1776 DT denotes the
T13042 1777-1787 NN denotes cerebellum
T13043 1788-1791 VBD denotes was
T13038 1792-1800 VBN denotes isolated
T13044 1801-1805 IN denotes from
T13045 1806-1807 DT denotes a
T13047 1808-1811 CD denotes six
T13048 1812-1817 NN denotes month
T13050 1817-1818 HYPH denotes -
T13049 1818-1821 JJ denotes old
T13046 1822-1827 NN denotes mouse
T13051 1828-1830 IN denotes of
T13052 1831-1835 DT denotes each
T13053 1836-1844 NN denotes genotype
T13054 1845-1848 CC denotes and
T13055 1849-1860 VBN denotes homogenised
T13056 1861-1863 IN denotes in
T13057 1864-1868 NN denotes cell
T13058 1869-1874 NN denotes lysis
T13059 1875-1881 NN denotes buffer
T13060 1882-1883 -LRB- denotes (
T13062 1883-1885 CD denotes 50
T13063 1886-1888 NN denotes mM
T13065 1889-1893 NN denotes Tris
T13066 1893-1894 HYPH denotes -
T13064 1894-1897 NN denotes HCl
T13067 1897-1899 , denotes ,
T13068 1899-1901 NN denotes pH
T13069 1902-1905 CD denotes 7.5
T13070 1905-1907 , denotes ,
T13071 1907-1910 CD denotes 100
T13072 1911-1913 NN denotes mM
T13073 1914-1918 NN denotes NaCl
T13074 1918-1920 , denotes ,
T13075 1920-1921 CD denotes 1
T13076 1921-1922 NN denotes %
T13077 1923-1925 NN denotes NP
T13078 1925-1926 HYPH denotes -
T13079 1926-1928 CD denotes 40
T13080 1928-1930 , denotes ,
T13081 1930-1938 NN denotes protease
T13082 1939-1948 NN denotes inhibitor
T13061 1949-1957 NN denotes cocktail
T13083 1958-1959 -LRB- denotes [
T13085 1959-1964 NNP denotes Roche
T13084 1965-1976 NNP denotes Diagnostics
T13086 1976-1977 -RRB- denotes ]
T13087 1977-1978 -RRB- denotes )
T13088 1978-1979 . denotes .
T13089 1979-2176 sentence denotes After removal of cell debris by centrifugation, the glycosylated proteins in the supernatant were concentrated by the affinity chromatography using Con A Sepharose 4B (Amersham Biosciences Corp.).
T13090 1980-1985 IN denotes After
T13092 1986-1993 NN denotes removal
T13093 1994-1996 IN denotes of
T13094 1997-2001 NN denotes cell
T13095 2002-2008 NN denotes debris
T13096 2009-2011 IN denotes by
T13097 2012-2026 NN denotes centrifugation
T13098 2026-2028 , denotes ,
T13099 2028-2031 DT denotes the
T13101 2032-2044 VBN denotes glycosylated
T13100 2045-2053 NN denotes proteins
T13102 2054-2056 IN denotes in
T13103 2057-2060 DT denotes the
T13104 2061-2072 NN denotes supernatant
T13105 2073-2077 VBD denotes were
T13091 2078-2090 VBN denotes concentrated
T13106 2091-2093 IN denotes by
T13107 2094-2097 DT denotes the
T13109 2098-2106 NN denotes affinity
T13108 2107-2121 NN denotes chromatography
T13110 2122-2127 VBG denotes using
T13111 2128-2131 NN denotes Con
T13113 2132-2133 NN denotes A
T13114 2134-2143 NN denotes Sepharose
T13112 2144-2146 NN denotes 4B
T13115 2147-2148 -LRB- denotes (
T13117 2148-2156 NNP denotes Amersham
T13118 2157-2168 NNP denotes Biosciences
T13116 2169-2174 NNP denotes Corp.
T13119 2174-2175 -RRB- denotes )
T13120 2175-2176 . denotes .
T13121 2176-2255 sentence denotes Each sample was separated on 10% SDS-PAGE, and transferred to a PVDF membrane.
T13122 2177-2181 DT denotes Each
T13123 2182-2188 NN denotes sample
T13125 2189-2192 VBD denotes was
T13124 2193-2202 VBN denotes separated
T13126 2203-2205 IN denotes on
T13127 2206-2208 CD denotes 10
T13128 2208-2209 NN denotes %
T13130 2210-2213 NN denotes SDS
T13131 2213-2214 HYPH denotes -
T13129 2214-2218 NN denotes PAGE
T13132 2218-2220 , denotes ,
T13133 2220-2223 CC denotes and
T13134 2224-2235 VBN denotes transferred
T13135 2236-2238 IN denotes to
T13136 2239-2240 DT denotes a
T13138 2241-2245 NN denotes PVDF
T13137 2246-2254 NN denotes membrane
T13139 2254-2255 . denotes .
T13140 2255-2364 sentence denotes The blot was then incubated with monoclonal antibody against the ADAM11 (U. S. Patent 5,631,351) at 1 μg/ml.
T13141 2256-2259 DT denotes The
T13142 2260-2264 NN denotes blot
T13144 2265-2268 VBD denotes was
T13145 2269-2273 RB denotes then
T13143 2274-2283 VBN denotes incubated
T13146 2284-2288 IN denotes with
T13147 2289-2299 JJ denotes monoclonal
T13148 2300-2308 NN denotes antibody
T13149 2309-2316 IN denotes against
T13150 2317-2320 DT denotes the
T13151 2321-2327 NN denotes ADAM11
T13152 2328-2329 -LRB- denotes (
T13154 2329-2331 NNP denotes U.
T13155 2332-2334 NNP denotes S.
T13153 2335-2341 NN denotes Patent
T13156 2342-2351 CD denotes 5,631,351
T13157 2351-2352 -RRB- denotes )
T13158 2353-2355 IN denotes at
T13159 2356-2357 CD denotes 1
T13160 2358-2360 NN denotes μg
T13161 2360-2361 SYM denotes /
T13162 2361-2363 NN denotes ml
T13163 2363-2364 . denotes .
T13164 2364-2531 sentence denotes Bound antibodies were visualised with horseradish peroxidase-labelled second antibody and an ECL-plus chemiluminescence detection system (Amersham Biosciences Corp.).
T13165 2365-2370 JJ denotes Bound
T13166 2371-2381 NNS denotes antibodies
T13168 2382-2386 VBD denotes were
T13167 2387-2397 VBN denotes visualised
T13169 2398-2402 IN denotes with
T13170 2403-2414 NN denotes horseradish
T13171 2415-2425 NN denotes peroxidase
T13173 2425-2426 HYPH denotes -
T13172 2426-2434 VBN denotes labelled
T13175 2435-2441 JJ denotes second
T13174 2442-2450 NN denotes antibody
T13176 2451-2454 CC denotes and
T13177 2455-2457 DT denotes an
T13179 2458-2461 NN denotes ECL
T13181 2461-2462 HYPH denotes -
T13180 2462-2466 NN denotes plus
T13182 2467-2484 NN denotes chemiluminescence
T13183 2485-2494 NN denotes detection
T13178 2495-2501 NN denotes system
T13184 2502-2503 -LRB- denotes (
T13186 2503-2511 NNP denotes Amersham
T13187 2512-2523 NNP denotes Biosciences
T13185 2524-2529 NNP denotes Corp.
T13188 2529-2530 -RRB- denotes )
T13189 2530-2531 . denotes .
T13416 2533-2544 JJ denotes Behavioural
T13417 2545-2553 NN denotes analysis
T13418 2553-2824 sentence denotes All animal procedures conformed to the Japanese regulations on animal care and use, following the Guideline for Animal Experimentation of the Japanese Association for Laboratory of Animal Science, and were approved by the Animal Care and Use Committee of Eisai Co., Ltd.
T13419 2554-2557 DT denotes All
T13421 2558-2564 NN denotes animal
T13420 2565-2575 NNS denotes procedures
T13422 2576-2585 VBD denotes conformed
T13423 2586-2588 IN denotes to
T13424 2589-2592 DT denotes the
T13426 2593-2601 JJ denotes Japanese
T13425 2602-2613 NNS denotes regulations
T13427 2614-2616 IN denotes on
T13428 2617-2623 NN denotes animal
T13429 2624-2628 NN denotes care
T13430 2629-2632 CC denotes and
T13431 2633-2636 NN denotes use
T13432 2636-2638 , denotes ,
T13433 2638-2647 VBG denotes following
T13434 2648-2651 DT denotes the
T13435 2652-2661 NNP denotes Guideline
T13436 2662-2665 IN denotes for
T13437 2666-2672 NNP denotes Animal
T13438 2673-2688 NNP denotes Experimentation
T13439 2689-2691 IN denotes of
T13440 2692-2695 DT denotes the
T13442 2696-2704 NNP denotes Japanese
T13441 2705-2716 NNP denotes Association
T13443 2717-2720 IN denotes for
T13444 2721-2731 NNP denotes Laboratory
T13445 2732-2734 IN denotes of
T13446 2735-2741 NNP denotes Animal
T13447 2742-2749 NNP denotes Science
T13448 2749-2751 , denotes ,
T13449 2751-2754 CC denotes and
T13450 2755-2759 VBD denotes were
T13451 2760-2768 VBN denotes approved
T13452 2769-2771 IN denotes by
T13453 2772-2775 DT denotes the
T13455 2776-2782 NNP denotes Animal
T13456 2783-2787 NNP denotes Care
T13457 2788-2791 CC denotes and
T13458 2792-2795 NNP denotes Use
T13454 2796-2805 NNP denotes Committee
T13459 2806-2808 IN denotes of
T13460 2809-2814 NNP denotes Eisai
T13461 2815-2818 NNP denotes Co.
T13462 2818-2820 , denotes ,
T13463 2820-2823 NNP denotes Ltd
T13464 2823-2824 . denotes .
T13465 2824-2991 sentence denotes For the present experiments, ADAM11-deficient male mice (backcrossed generations into C57BL/6NCrj, n = 14) were used in all behavioural analyses (22–24 weeks of age).
T13466 2825-2828 IN denotes For
T13468 2829-2832 DT denotes the
T13470 2833-2840 JJ denotes present
T13469 2841-2852 NNS denotes experiments
T13471 2852-2854 , denotes ,
T13472 2854-2860 NN denotes ADAM11
T13474 2860-2861 HYPH denotes -
T13473 2861-2870 JJ denotes deficient
T13476 2871-2875 JJ denotes male
T13475 2876-2880 NNS denotes mice
T13477 2881-2882 -LRB- denotes (
T13479 2882-2893 VBN denotes backcrossed
T13478 2894-2905 NNS denotes generations
T13480 2906-2910 IN denotes into
T13481 2911-2916 NN denotes C57BL
T13483 2916-2917 HYPH denotes /
T13482 2917-2922 NN denotes 6NCrj
T13484 2922-2924 , denotes ,
T13485 2924-2925 NN denotes n
T13487 2926-2927 SYM denotes =
T13486 2928-2930 CD denotes 14
T13488 2930-2931 -RRB- denotes )
T13489 2932-2936 VBD denotes were
T13467 2937-2941 VBN denotes used
T13490 2942-2944 IN denotes in
T13491 2945-2948 DT denotes all
T13493 2949-2960 JJ denotes behavioural
T13492 2961-2969 NNS denotes analyses
T13494 2970-2971 -LRB- denotes (
T13496 2971-2973 CD denotes 22
T13498 2973-2974 SYM denotes –
T13497 2974-2976 CD denotes 24
T13495 2977-2982 NNS denotes weeks
T13499 2983-2985 IN denotes of
T13500 2986-2989 NN denotes age
T13501 2989-2990 -RRB- denotes )
T13502 2990-2991 . denotes .
T13503 2991-3122 sentence denotes All of the behavioural studies were conducted between 10:00 and 16:00 by a well-trained experimenter blind to the mouse genotypes.
T13504 2992-2995 DT denotes All
T13506 2996-2998 IN denotes of
T13507 2999-3002 DT denotes the
T13509 3003-3014 JJ denotes behavioural
T13508 3015-3022 NNS denotes studies
T13510 3023-3027 VBD denotes were
T13505 3028-3037 VBN denotes conducted
T13511 3038-3045 IN denotes between
T13512 3046-3048 CD denotes 10
T13514 3048-3049 : denotes :
T13513 3049-3051 CD denotes 00
T13515 3052-3055 CC denotes and
T13516 3056-3058 CD denotes 16
T13518 3058-3059 : denotes :
T13517 3059-3061 CD denotes 00
T13519 3062-3064 IN denotes by
T13520 3065-3066 DT denotes a
T13522 3067-3071 RB denotes well
T13524 3071-3072 HYPH denotes -
T13523 3072-3079 VBN denotes trained
T13521 3080-3092 NN denotes experimenter
T13525 3093-3098 JJ denotes blind
T13526 3099-3101 IN denotes to
T13527 3102-3105 DT denotes the
T13529 3106-3111 NN denotes mouse
T13528 3112-3121 NNS denotes genotypes
T13530 3121-3122 . denotes .
T13661 3124-3135 JJ denotes Spontaneous
T13663 3136-3141 NN denotes motor
T13662 3142-3150 NN denotes activity
T13664 3150-3393 sentence denotes Locomotor activity in a novel environment was measured by introducing mice (+/+, +/- and -/-; n = 8, 8 and 8, respectively) for 90 min in a Plexiglas box (30 × 20 × 13 cm) using a VERSAMAX equipment and software (Accuscan, Columbus, OH, USA).
T13665 3151-3160 NN denotes Locomotor
T13666 3161-3169 NN denotes activity
T13668 3170-3172 IN denotes in
T13669 3173-3174 DT denotes a
T13671 3175-3180 JJ denotes novel
T13670 3181-3192 NN denotes environment
T13672 3193-3196 VBD denotes was
T13667 3197-3205 VBN denotes measured
T13673 3206-3208 IN denotes by
T13674 3209-3220 VBG denotes introducing
T13675 3221-3225 NNS denotes mice
T13676 3226-3227 -LRB- denotes (
T13678 3227-3228 SYM denotes +
T13679 3228-3229 HYPH denotes /
T13677 3229-3230 SYM denotes +
T13680 3230-3232 , denotes ,
T13681 3232-3233 SYM denotes +
T13683 3233-3234 HYPH denotes /
T13682 3234-3235 SYM denotes -
T13684 3236-3239 CC denotes and
T13685 3240-3241 SYM denotes -
T13687 3241-3242 HYPH denotes /
T13686 3242-3243 SYM denotes -
T13688 3243-3244 : denotes ;
T13689 3245-3246 NN denotes n
T13691 3247-3248 SYM denotes =
T13690 3249-3250 CD denotes 8
T13692 3250-3252 , denotes ,
T13693 3252-3253 CD denotes 8
T13694 3254-3257 CC denotes and
T13695 3258-3259 CD denotes 8
T13696 3259-3261 , denotes ,
T13697 3261-3273 RB denotes respectively
T13698 3273-3274 -RRB- denotes )
T13699 3275-3278 IN denotes for
T13700 3279-3281 CD denotes 90
T13701 3282-3285 NN denotes min
T13702 3286-3288 IN denotes in
T13703 3289-3290 DT denotes a
T13705 3291-3300 NN denotes Plexiglas
T13704 3301-3304 NN denotes box
T13706 3305-3306 -LRB- denotes (
T13708 3306-3308 CD denotes 30
T13709 3309-3310 SYM denotes ×
T13710 3311-3313 CD denotes 20
T13711 3314-3315 SYM denotes ×
T13712 3316-3318 CD denotes 13
T13707 3319-3321 NN denotes cm
T13713 3321-3322 -RRB- denotes )
T13714 3323-3328 VBG denotes using
T13715 3329-3330 DT denotes a
T13717 3331-3339 NNP denotes VERSAMAX
T13716 3340-3349 NN denotes equipment
T13718 3350-3353 CC denotes and
T13719 3354-3362 NN denotes software
T13720 3363-3364 -LRB- denotes (
T13721 3364-3372 NNP denotes Accuscan
T13722 3372-3374 , denotes ,
T13723 3374-3382 NNP denotes Columbus
T13724 3382-3384 , denotes ,
T13725 3384-3386 NNP denotes OH
T13726 3386-3388 , denotes ,
T13727 3388-3391 NNP denotes USA
T13728 3391-3392 -RRB- denotes )
T13729 3392-3393 . denotes .
T14364 3395-3400 NN denotes Water
T14365 3401-3405 NN denotes maze
T14366 3406-3410 NN denotes task
T14367 3410-3511 sentence denotes Spatial learning was assessed by three variants of the Morris water maze task [27] adapted for mice.
T14368 3411-3418 JJ denotes Spatial
T14369 3419-3427 NN denotes learning
T14371 3428-3431 VBD denotes was
T14370 3432-3440 VBN denotes assessed
T14372 3441-3443 IN denotes by
T14373 3444-3449 CD denotes three
T14374 3450-3458 NNS denotes variants
T14375 3459-3461 IN denotes of
T14376 3462-3465 DT denotes the
T14378 3466-3472 NNP denotes Morris
T14379 3473-3478 NN denotes water
T14380 3479-3483 NN denotes maze
T14377 3484-3488 NN denotes task
T14381 3489-3490 -LRB- denotes [
T14382 3490-3492 CD denotes 27
T14383 3492-3493 -RRB- denotes ]
T14384 3494-3501 VBN denotes adapted
T14385 3502-3505 IN denotes for
T14386 3506-3510 NNS denotes mice
T14387 3510-3511 . denotes .
T14388 3511-3671 sentence denotes Hidden platform task: The pool was divided into four quadrants with four starting locations, called north, east, south and west, at equal distances to the rim.
T14389 3512-3518 JJ denotes Hidden
T14391 3519-3527 NN denotes platform
T14390 3528-3532 NN denotes task
T14393 3532-3534 : denotes :
T14394 3534-3537 DT denotes The
T14395 3538-3542 NN denotes pool
T14396 3543-3546 VBD denotes was
T14392 3547-3554 VBN denotes divided
T14397 3555-3559 IN denotes into
T14398 3560-3564 CD denotes four
T14399 3565-3574 NNS denotes quadrants
T14400 3575-3579 IN denotes with
T14402 3580-3584 CD denotes four
T14404 3585-3593 NN denotes starting
T14403 3594-3603 NNS denotes locations
T14405 3603-3605 , denotes ,
T14406 3605-3611 VBN denotes called
T14407 3612-3617 NN denotes north
T14408 3617-3619 , denotes ,
T14409 3619-3623 NN denotes east
T14410 3623-3625 , denotes ,
T14411 3625-3630 NN denotes south
T14412 3631-3634 CC denotes and
T14413 3635-3639 NN denotes west
T14414 3639-3641 , denotes ,
T14401 3641-3643 IN denotes at
T14415 3644-3649 JJ denotes equal
T14416 3650-3659 NNS denotes distances
T14417 3660-3662 IN denotes to
T14418 3663-3666 DT denotes the
T14419 3667-3670 NN denotes rim
T14420 3670-3671 . denotes .
T14421 3671-3847 sentence denotes A circular, transparent acrylic platform (diameter 8 cm) was submerged 1 cm below the surface of the water in the centre of the southeast quadrant for each trial of this task.
T14422 3672-3673 DT denotes A
T14424 3674-3682 JJ denotes circular
T14425 3682-3684 , denotes ,
T14426 3684-3695 JJ denotes transparent
T14427 3696-3703 JJ denotes acrylic
T14423 3704-3712 NN denotes platform
T14429 3713-3714 -LRB- denotes (
T14431 3714-3722 NN denotes diameter
T14432 3723-3724 CD denotes 8
T14430 3725-3727 NN denotes cm
T14433 3727-3728 -RRB- denotes )
T14434 3729-3732 VBD denotes was
T14428 3733-3742 VBN denotes submerged
T14435 3743-3744 CD denotes 1
T14436 3745-3747 NN denotes cm
T14437 3748-3753 IN denotes below
T14438 3754-3757 DT denotes the
T14439 3758-3765 NN denotes surface
T14440 3766-3768 IN denotes of
T14441 3769-3772 DT denotes the
T14442 3773-3778 NN denotes water
T14443 3779-3781 IN denotes in
T14444 3782-3785 DT denotes the
T14445 3786-3792 NN denotes centre
T14446 3793-3795 IN denotes of
T14447 3796-3799 DT denotes the
T14449 3800-3809 NN denotes southeast
T14448 3810-3818 NN denotes quadrant
T14450 3819-3822 IN denotes for
T14451 3823-3827 DT denotes each
T14452 3828-3833 NN denotes trial
T14453 3834-3836 IN denotes of
T14454 3837-3841 DT denotes this
T14455 3842-3846 NN denotes task
T14456 3846-3847 . denotes .
T14457 3847-3978 sentence denotes Each mouse was allowed to swim for 60 sec and the time required to reach the platform (escape latency) was recorded in each trial.
T14458 3848-3852 DT denotes Each
T14459 3853-3858 NN denotes mouse
T14461 3859-3862 VBD denotes was
T14460 3863-3870 VBN denotes allowed
T14462 3871-3873 TO denotes to
T14463 3874-3878 VB denotes swim
T14464 3879-3882 IN denotes for
T14465 3883-3885 CD denotes 60
T14466 3886-3889 NN denotes sec
T14467 3890-3893 CC denotes and
T14468 3894-3897 DT denotes the
T14469 3898-3902 NN denotes time
T14471 3903-3911 VBN denotes required
T14472 3912-3914 TO denotes to
T14473 3915-3920 VB denotes reach
T14474 3921-3924 DT denotes the
T14475 3925-3933 NN denotes platform
T14476 3934-3935 -LRB- denotes (
T14477 3935-3941 NN denotes escape
T14478 3942-3949 NN denotes latency
T14479 3949-3950 -RRB- denotes )
T14480 3951-3954 VBD denotes was
T14470 3955-3963 VBN denotes recorded
T14481 3964-3966 IN denotes in
T14482 3967-3971 DT denotes each
T14483 3972-3977 NN denotes trial
T14484 3977-3978 . denotes .
T14485 3978-4073 sentence denotes In total this task consisted of four trials per day over 9 days (1 min inter-trial intervals).
T14486 3979-3981 IN denotes In
T14488 3982-3987 JJ denotes total
T14489 3988-3992 DT denotes this
T14490 3993-3997 NN denotes task
T14487 3998-4007 VBD denotes consisted
T14491 4008-4010 IN denotes of
T14492 4011-4015 CD denotes four
T14493 4016-4022 NNS denotes trials
T14494 4023-4026 IN denotes per
T14495 4027-4030 NN denotes day
T14496 4031-4035 IN denotes over
T14497 4036-4037 CD denotes 9
T14498 4038-4042 NNS denotes days
T14499 4043-4044 -LRB- denotes (
T14501 4044-4045 CD denotes 1
T14502 4046-4049 NN denotes min
T14503 4050-4061 JJ denotes inter-trial
T14500 4062-4071 NNS denotes intervals
T14504 4071-4072 -RRB- denotes )
T14505 4072-4073 . denotes .
T14506 4073-4167 sentence denotes Each trial was initiated by randomly placing an animal in one of the four starting locations.
T14507 4074-4078 DT denotes Each
T14508 4079-4084 NN denotes trial
T14510 4085-4088 VBD denotes was
T14509 4089-4098 VBN denotes initiated
T14511 4099-4101 IN denotes by
T14512 4102-4110 RB denotes randomly
T14513 4111-4118 VBG denotes placing
T14514 4119-4121 DT denotes an
T14515 4122-4128 NN denotes animal
T14516 4129-4131 IN denotes in
T14517 4132-4135 CD denotes one
T14518 4136-4138 IN denotes of
T14519 4139-4142 DT denotes the
T14521 4143-4147 CD denotes four
T14522 4148-4156 NN denotes starting
T14520 4157-4166 NNS denotes locations
T14523 4166-4167 . denotes .
T14524 4167-4268 sentence denotes Probe trial: A single probe trial was carried out after the hidden platform task had been completed.
T14525 4168-4173 NN denotes Probe
T14526 4174-4179 NN denotes trial
T14528 4179-4181 : denotes :
T14529 4181-4182 DT denotes A
T14531 4183-4189 JJ denotes single
T14532 4190-4195 NN denotes probe
T14530 4196-4201 NN denotes trial
T14533 4202-4205 VBD denotes was
T14527 4206-4213 VBN denotes carried
T14534 4214-4217 RP denotes out
T14535 4218-4223 IN denotes after
T14537 4224-4227 DT denotes the
T14539 4228-4234 JJ denotes hidden
T14540 4235-4243 NN denotes platform
T14538 4244-4248 NN denotes task
T14541 4249-4252 VBD denotes had
T14542 4253-4257 VBN denotes been
T14536 4258-4267 VBN denotes completed
T14543 4267-4268 . denotes .
T14544 4268-4448 sentence denotes In this trial, the platform was removed and the movement of each mouse was monitored using a computer-based video tracking system (BTA-2; Muromachi Kikai Co., Ltd., Tokyo, Japan).
T14545 4269-4271 IN denotes In
T14547 4272-4276 DT denotes this
T14548 4277-4282 NN denotes trial
T14549 4282-4284 , denotes ,
T14550 4284-4287 DT denotes the
T14551 4288-4296 NN denotes platform
T14552 4297-4300 VBD denotes was
T14546 4301-4308 VBN denotes removed
T14553 4309-4312 CC denotes and
T14554 4313-4316 DT denotes the
T14555 4317-4325 NN denotes movement
T14557 4326-4328 IN denotes of
T14558 4329-4333 DT denotes each
T14559 4334-4339 NN denotes mouse
T14560 4340-4343 VBD denotes was
T14556 4344-4353 VBN denotes monitored
T14561 4354-4359 VBG denotes using
T14562 4360-4361 DT denotes a
T14564 4362-4370 NN denotes computer
T14566 4370-4371 HYPH denotes -
T14565 4371-4376 VBN denotes based
T14567 4377-4382 NN denotes video
T14568 4383-4391 NN denotes tracking
T14563 4392-4398 NN denotes system
T14569 4399-4400 -LRB- denotes (
T14571 4400-4403 NN denotes BTA
T14572 4403-4404 HYPH denotes -
T14573 4404-4405 CD denotes 2
T14574 4405-4406 : denotes ;
T14575 4407-4416 NNP denotes Muromachi
T14576 4417-4422 NNP denotes Kikai
T14570 4423-4426 NNP denotes Co.
T14577 4426-4428 , denotes ,
T14578 4428-4432 NNP denotes Ltd.
T14579 4432-4434 , denotes ,
T14580 4434-4439 NNP denotes Tokyo
T14581 4439-4441 , denotes ,
T14582 4441-4446 NNP denotes Japan
T14583 4446-4447 -RRB- denotes )
T14584 4447-4448 . denotes .
T14585 4448-4542 sentence denotes Each mouse was placed into the northwest quadrant of the pool and allowed to swim for 60 sec.
T14586 4449-4453 DT denotes Each
T14587 4454-4459 NN denotes mouse
T14589 4460-4463 VBD denotes was
T14588 4464-4470 VBN denotes placed
T14590 4471-4475 IN denotes into
T14591 4476-4479 DT denotes the
T14593 4480-4489 NN denotes northwest
T14592 4490-4498 NN denotes quadrant
T14594 4499-4501 IN denotes of
T14595 4502-4505 DT denotes the
T14596 4506-4510 NN denotes pool
T14597 4511-4514 CC denotes and
T14598 4515-4522 VBN denotes allowed
T14599 4523-4525 TO denotes to
T14600 4526-4530 VB denotes swim
T14601 4531-4534 IN denotes for
T14602 4535-4537 CD denotes 60
T14603 4538-4541 NN denotes sec
T14604 4541-4542 . denotes .
T14605 4542-4631 sentence denotes Swim path length and the time spent in the trained (southeast) quadrant were calculated.
T14606 4543-4547 NN denotes Swim
T14607 4548-4552 NN denotes path
T14608 4553-4559 NN denotes length
T14610 4560-4563 CC denotes and
T14611 4564-4567 DT denotes the
T14612 4568-4572 NN denotes time
T14613 4573-4578 VBN denotes spent
T14614 4579-4581 IN denotes in
T14615 4582-4585 DT denotes the
T14617 4586-4593 VBN denotes trained
T14618 4594-4595 -LRB- denotes (
T14619 4595-4604 NN denotes southeast
T14620 4604-4605 -RRB- denotes )
T14616 4606-4614 NN denotes quadrant
T14621 4615-4619 VBD denotes were
T14609 4620-4630 VBN denotes calculated
T14622 4630-4631 . denotes .
T14623 4631-4799 sentence denotes Visible platform task: In this task, the circular platform was made visible by attaching a black board to it and the mouse was required to locate the visible platform.
T14624 4632-4639 JJ denotes Visible
T14626 4640-4648 NN denotes platform
T14625 4649-4653 NN denotes task
T14628 4653-4655 : denotes :
T14629 4655-4657 IN denotes In
T14630 4658-4662 DT denotes this
T14631 4663-4667 NN denotes task
T14632 4667-4669 , denotes ,
T14633 4669-4672 DT denotes the
T14635 4673-4681 JJ denotes circular
T14634 4682-4690 NN denotes platform
T14636 4691-4694 VBD denotes was
T14627 4695-4699 VBN denotes made
T14637 4700-4707 JJ denotes visible
T14638 4708-4710 IN denotes by
T14639 4711-4720 VBG denotes attaching
T14640 4721-4722 DT denotes a
T14642 4723-4728 JJ denotes black
T14641 4729-4734 NN denotes board
T14643 4735-4737 IN denotes to
T14644 4738-4740 PRP denotes it
T14645 4741-4744 CC denotes and
T14646 4745-4748 DT denotes the
T14647 4749-4754 NN denotes mouse
T14649 4755-4758 VBD denotes was
T14648 4759-4767 VBN denotes required
T14650 4768-4770 TO denotes to
T14651 4771-4777 VB denotes locate
T14652 4778-4781 DT denotes the
T14654 4782-4789 JJ denotes visible
T14653 4790-4798 NN denotes platform
T14655 4798-4799 . denotes .
T14656 4799-4945 sentence denotes The mouse was allowed to swim for 60 sec and this task consisted of four trials per day for three consecutive days (1 min inter-trial intervals).
T14657 4800-4803 DT denotes The
T14658 4804-4809 NN denotes mouse
T14660 4810-4813 VBD denotes was
T14659 4814-4821 VBN denotes allowed
T14661 4822-4824 TO denotes to
T14662 4825-4829 VB denotes swim
T14663 4830-4833 IN denotes for
T14664 4834-4836 CD denotes 60
T14665 4837-4840 NN denotes sec
T14666 4841-4844 CC denotes and
T14667 4845-4849 DT denotes this
T14668 4850-4854 NN denotes task
T14669 4855-4864 VBD denotes consisted
T14670 4865-4867 IN denotes of
T14671 4868-4872 CD denotes four
T14672 4873-4879 NNS denotes trials
T14673 4880-4883 IN denotes per
T14674 4884-4887 NN denotes day
T14675 4888-4891 IN denotes for
T14676 4892-4897 CD denotes three
T14678 4898-4909 JJ denotes consecutive
T14677 4910-4914 NNS denotes days
T14679 4915-4916 -LRB- denotes (
T14681 4916-4917 CD denotes 1
T14682 4918-4921 NN denotes min
T14683 4922-4933 JJ denotes inter-trial
T14680 4934-4943 NNS denotes intervals
T14684 4943-4944 -RRB- denotes )
T14685 4944-4945 . denotes .
T14686 4945-5039 sentence denotes Each trial was initiated by randomly placing an animal in one of the four starting locations.
T14687 4946-4950 DT denotes Each
T14688 4951-4956 NN denotes trial
T14690 4957-4960 VBD denotes was
T14689 4961-4970 VBN denotes initiated
T14691 4971-4973 IN denotes by
T14692 4974-4982 RB denotes randomly
T14693 4983-4990 VBG denotes placing
T14694 4991-4993 DT denotes an
T14695 4994-5000 NN denotes animal
T14696 5001-5003 IN denotes in
T14697 5004-5007 CD denotes one
T14698 5008-5010 IN denotes of
T14699 5011-5014 DT denotes the
T14701 5015-5019 CD denotes four
T14702 5020-5028 NN denotes starting
T14700 5029-5038 NNS denotes locations
T14703 5038-5039 . denotes .
T14924 5041-5049 VBG denotes Rotating
T14925 5050-5053 NN denotes rod
T14926 5054-5058 NN denotes test
T14927 5058-5290 sentence denotes Motor coordination was assessed with a rotating rod apparatus (KN-75, Natsume Seisakujo, Tokyo, Japan), which consisted of a plastic rod (3 cm diameter, 8 cm long) with a gritted surface flanked by two large discs (40 cm diameter).
T14928 5059-5064 NN denotes Motor
T14929 5065-5077 NN denotes coordination
T14931 5078-5081 VBD denotes was
T14930 5082-5090 VBN denotes assessed
T14932 5091-5095 IN denotes with
T14933 5096-5097 DT denotes a
T14935 5098-5106 VBG denotes rotating
T14936 5107-5110 NN denotes rod
T14934 5111-5120 NN denotes apparatus
T14937 5121-5122 -LRB- denotes (
T14939 5122-5124 NN denotes KN
T14940 5124-5125 HYPH denotes -
T14941 5125-5127 CD denotes 75
T14942 5127-5129 , denotes ,
T14943 5129-5136 NNP denotes Natsume
T14938 5137-5146 NNP denotes Seisakujo
T14944 5146-5148 , denotes ,
T14945 5148-5153 NNP denotes Tokyo
T14946 5153-5155 , denotes ,
T14947 5155-5160 NNP denotes Japan
T14948 5160-5161 -RRB- denotes )
T14949 5161-5163 , denotes ,
T14950 5163-5168 WDT denotes which
T14951 5169-5178 VBD denotes consisted
T14952 5179-5181 IN denotes of
T14953 5182-5183 DT denotes a
T14955 5184-5191 NN denotes plastic
T14954 5192-5195 NN denotes rod
T14956 5196-5197 -LRB- denotes (
T14958 5197-5198 CD denotes 3
T14959 5199-5201 NN denotes cm
T14957 5202-5210 NN denotes diameter
T14960 5210-5212 , denotes ,
T14961 5212-5213 CD denotes 8
T14962 5214-5216 NN denotes cm
T14963 5217-5221 JJ denotes long
T14964 5221-5222 -RRB- denotes )
T14965 5223-5227 IN denotes with
T14966 5228-5229 DT denotes a
T14968 5230-5237 JJ denotes gritted
T14967 5238-5245 NN denotes surface
T14969 5246-5253 VBN denotes flanked
T14970 5254-5256 IN denotes by
T14971 5257-5260 CD denotes two
T14973 5261-5266 JJ denotes large
T14972 5267-5272 NNS denotes discs
T14974 5273-5274 -LRB- denotes (
T14976 5274-5276 CD denotes 40
T14977 5277-5279 NN denotes cm
T14975 5280-5288 NN denotes diameter
T14978 5288-5289 -RRB- denotes )
T14979 5289-5290 . denotes .
T14980 5290-5474 sentence denotes The mice were first placed on the stationary rod for 4 successive trials, followed by 4 trials at a rotation speed of 5 rpm, 8 trials at 10 rpm, and 20 trials at 15 rpm in that order.
T14981 5291-5294 DT denotes The
T14982 5295-5299 NNS denotes mice
T14984 5300-5304 VBD denotes were
T14985 5305-5310 RB denotes first
T14983 5311-5317 VBN denotes placed
T14986 5318-5320 IN denotes on
T14987 5321-5324 DT denotes the
T14989 5325-5335 JJ denotes stationary
T14988 5336-5339 NN denotes rod
T14990 5340-5343 IN denotes for
T14991 5344-5345 CD denotes 4
T14993 5346-5356 JJ denotes successive
T14992 5357-5363 NNS denotes trials
T14994 5363-5365 , denotes ,
T14995 5365-5373 VBN denotes followed
T14996 5374-5376 IN denotes by
T14997 5377-5378 CD denotes 4
T14998 5379-5385 NNS denotes trials
T14999 5386-5388 IN denotes at
T15000 5389-5390 DT denotes a
T15002 5391-5399 NN denotes rotation
T15001 5400-5405 NN denotes speed
T15003 5406-5408 IN denotes of
T15004 5409-5410 CD denotes 5
T15005 5411-5414 NN denotes rpm
T15006 5414-5416 , denotes ,
T15007 5416-5417 CD denotes 8
T15008 5418-5424 NNS denotes trials
T15009 5425-5427 IN denotes at
T15010 5428-5430 CD denotes 10
T15011 5431-5434 NN denotes rpm
T15012 5434-5436 , denotes ,
T15013 5436-5439 CC denotes and
T15014 5440-5442 CD denotes 20
T15015 5443-5449 NNS denotes trials
T15016 5450-5452 IN denotes at
T15017 5453-5455 CD denotes 15
T15018 5456-5459 NN denotes rpm
T15019 5460-5462 IN denotes in
T15020 5463-5467 DT denotes that
T15021 5468-5473 NN denotes order
T15022 5473-5474 . denotes .
T15023 5474-5531 sentence denotes Latency until a fall occurred was monitored for 120 sec.
T15024 5475-5482 NN denotes Latency
T15026 5483-5488 IN denotes until
T15028 5489-5490 DT denotes a
T15029 5491-5495 NN denotes fall
T15027 5496-5504 VBD denotes occurred
T15030 5505-5508 VBD denotes was
T15025 5509-5518 VBN denotes monitored
T15031 5519-5522 IN denotes for
T15032 5523-5526 CD denotes 120
T15033 5527-5530 NN denotes sec
T15034 5530-5531 . denotes .
T15035 5531-5592 sentence denotes Intra-trial intervals for each animal were more than 20 min.
T15036 5532-5543 JJ denotes Intra-trial
T15037 5544-5553 NNS denotes intervals
T15039 5554-5557 IN denotes for
T15040 5558-5562 DT denotes each
T15041 5563-5569 NN denotes animal
T15038 5570-5574 VBD denotes were
T15042 5575-5579 JJR denotes more
T15044 5580-5584 IN denotes than
T15043 5585-5587 CD denotes 20
T15045 5588-5591 NN denotes min
T15046 5591-5592 . denotes .
T15164 5594-5602 NN denotes Traction
T15165 5603-5607 NN denotes test
T15166 5607-5713 sentence denotes The grip strength of each mouse was measured with a traction apparatus (FU-1, Muromachi Kikai Co., Ltd.).
T15167 5608-5611 DT denotes The
T15169 5612-5616 NN denotes grip
T15168 5617-5625 NN denotes strength
T15171 5626-5628 IN denotes of
T15172 5629-5633 DT denotes each
T15173 5634-5639 NN denotes mouse
T15174 5640-5643 VBD denotes was
T15170 5644-5652 VBN denotes measured
T15175 5653-5657 IN denotes with
T15176 5658-5659 DT denotes a
T15178 5660-5668 NN denotes traction
T15177 5669-5678 NN denotes apparatus
T15179 5679-5680 -LRB- denotes (
T15181 5680-5682 NN denotes FU
T15182 5682-5683 HYPH denotes -
T15183 5683-5684 CD denotes 1
T15184 5684-5686 , denotes ,
T15185 5686-5695 NNP denotes Muromachi
T15186 5696-5701 NNP denotes Kikai
T15180 5702-5705 NNP denotes Co.
T15187 5705-5707 , denotes ,
T15188 5707-5711 NNP denotes Ltd.
T15189 5711-5712 -RRB- denotes )
T15190 5712-5713 . denotes .
T15191 5713-5833 sentence denotes Each mouse was made to grasp the attached bar (2 mm diameter) with the forepaws and was slowly pulled back by the tail.
T15192 5714-5718 DT denotes Each
T15193 5719-5724 NN denotes mouse
T15195 5725-5728 VBD denotes was
T15194 5729-5733 VBN denotes made
T15196 5734-5736 TO denotes to
T15197 5737-5742 VB denotes grasp
T15198 5743-5746 DT denotes the
T15200 5747-5755 VBN denotes attached
T15199 5756-5759 NN denotes bar
T15201 5760-5761 -LRB- denotes (
T15203 5761-5762 CD denotes 2
T15204 5763-5765 NN denotes mm
T15202 5766-5774 NN denotes diameter
T15205 5774-5775 -RRB- denotes )
T15206 5776-5780 IN denotes with
T15207 5781-5784 DT denotes the
T15208 5785-5793 NNS denotes forepaws
T15209 5794-5797 CC denotes and
T15210 5798-5801 VBD denotes was
T15212 5802-5808 RB denotes slowly
T15211 5809-5815 VBN denotes pulled
T15213 5816-5820 RB denotes back
T15214 5821-5823 IN denotes by
T15215 5824-5827 DT denotes the
T15216 5828-5832 NN denotes tail
T15217 5832-5833 . denotes .
T15218 5833-5882 sentence denotes The maximum tension before release was recorded.
T15219 5834-5837 DT denotes The
T15221 5838-5845 JJ denotes maximum
T15220 5846-5853 NN denotes tension
T15223 5854-5860 IN denotes before
T15224 5861-5868 NN denotes release
T15225 5869-5872 VBD denotes was
T15222 5873-5881 VBN denotes recorded
T15226 5881-5882 . denotes .
T15486 5884-5888 NN denotes Wire
T15487 5889-5899 NN denotes suspension
T15488 5900-5904 NN denotes test
T15489 5904-6097 sentence denotes A mouse was placed on a stainless bar (50 cm length, 2 mm diameter, elevated up to 37 cm from a surface) at a point midway between the supports and observed for 30 sec in four separate trials.
T15490 5905-5906 DT denotes A
T15491 5907-5912 NN denotes mouse
T15493 5913-5916 VBD denotes was
T15492 5917-5923 VBN denotes placed
T15494 5924-5926 IN denotes on
T15495 5927-5928 DT denotes a
T15497 5929-5938 JJ denotes stainless
T15496 5939-5942 NN denotes bar
T15498 5943-5944 -LRB- denotes (
T15500 5944-5946 CD denotes 50
T15501 5947-5949 NN denotes cm
T15502 5950-5956 NN denotes length
T15503 5956-5958 , denotes ,
T15504 5958-5959 CD denotes 2
T15505 5960-5962 NN denotes mm
T15499 5963-5971 NN denotes diameter
T15506 5971-5973 , denotes ,
T15507 5973-5981 VBN denotes elevated
T15508 5982-5984 RB denotes up
T15510 5985-5987 IN denotes to
T15509 5988-5990 CD denotes 37
T15511 5991-5993 NN denotes cm
T15512 5994-5998 IN denotes from
T15513 5999-6000 DT denotes a
T15514 6001-6008 NN denotes surface
T15515 6008-6009 -RRB- denotes )
T15516 6010-6012 IN denotes at
T15517 6013-6014 DT denotes a
T15519 6015-6020 NN denotes point
T15518 6021-6027 NN denotes midway
T15520 6028-6035 IN denotes between
T15521 6036-6039 DT denotes the
T15522 6040-6048 NNS denotes supports
T15523 6049-6052 CC denotes and
T15524 6053-6061 VBN denotes observed
T15525 6062-6065 IN denotes for
T15526 6066-6068 CD denotes 30
T15527 6069-6072 NN denotes sec
T15528 6073-6075 IN denotes in
T15529 6076-6080 CD denotes four
T15531 6081-6089 JJ denotes separate
T15530 6090-6096 NNS denotes trials
T15532 6096-6097 . denotes .
T15533 6097-6476 sentence denotes The amount of time spent hanging was recorded and scored according to the following system [41]: 0, fell off; 1, hung onto the bar with two forepaws; 2, in addition to 1, attempted to climb onto the bar; 3, hung onto the bar with two forepaws and one or both hind paws; 4, hung onto the bar with all four paws with tail wrapped around the bar; 5, escaped to one of the supports.
T15534 6098-6101 DT denotes The
T15535 6102-6108 NN denotes amount
T15537 6109-6111 IN denotes of
T15538 6112-6116 NN denotes time
T15539 6117-6122 VBN denotes spent
T15540 6123-6130 VBG denotes hanging
T15541 6131-6134 VBD denotes was
T15536 6135-6143 VBN denotes recorded
T15542 6144-6147 CC denotes and
T15543 6148-6154 VBN denotes scored
T15544 6155-6164 VBG denotes according
T15545 6165-6167 IN denotes to
T15546 6168-6171 DT denotes the
T15548 6172-6181 VBG denotes following
T15547 6182-6188 NN denotes system
T15549 6189-6190 -LRB- denotes [
T15550 6190-6192 CD denotes 41
T15551 6192-6193 -RRB- denotes ]
T15552 6193-6195 : denotes :
T15553 6195-6196 CD denotes 0
T15555 6196-6198 , denotes ,
T15554 6198-6202 VBD denotes fell
T15557 6203-6206 RP denotes off
T15558 6206-6207 : denotes ;
T15559 6208-6209 CD denotes 1
T15561 6209-6211 , denotes ,
T15560 6211-6215 VBD denotes hung
T15562 6216-6220 IN denotes onto
T15563 6221-6224 DT denotes the
T15564 6225-6228 NN denotes bar
T15565 6229-6233 IN denotes with
T15566 6234-6237 CD denotes two
T15567 6238-6246 NNS denotes forepaws
T15568 6246-6247 : denotes ;
T15569 6248-6249 CD denotes 2
T15571 6249-6251 , denotes ,
T15572 6251-6253 IN denotes in
T15573 6254-6262 NN denotes addition
T15574 6263-6265 IN denotes to
T15575 6266-6267 CD denotes 1
T15576 6267-6269 , denotes ,
T15570 6269-6278 VBD denotes attempted
T15577 6279-6281 TO denotes to
T15578 6282-6287 VB denotes climb
T15579 6288-6292 IN denotes onto
T15580 6293-6296 DT denotes the
T15581 6297-6300 NN denotes bar
T15582 6300-6301 : denotes ;
T15583 6302-6303 CD denotes 3
T15585 6303-6305 , denotes ,
T15584 6305-6309 VBD denotes hung
T15586 6310-6314 IN denotes onto
T15587 6315-6318 DT denotes the
T15588 6319-6322 NN denotes bar
T15589 6323-6327 IN denotes with
T15590 6328-6331 CD denotes two
T15591 6332-6340 NNS denotes forepaws
T15592 6341-6344 CC denotes and
T15593 6345-6348 CD denotes one
T15595 6349-6351 CC denotes or
T15596 6352-6356 CC denotes both
T15597 6357-6361 NN denotes hind
T15594 6362-6366 NNS denotes paws
T15598 6366-6367 : denotes ;
T15599 6368-6369 CD denotes 4
T15601 6369-6371 , denotes ,
T15600 6371-6375 VBD denotes hung
T15602 6376-6380 IN denotes onto
T15603 6381-6384 DT denotes the
T15604 6385-6388 NN denotes bar
T15605 6389-6393 IN denotes with
T15606 6394-6397 DT denotes all
T15608 6398-6402 CD denotes four
T15607 6403-6407 NNS denotes paws
T15609 6408-6412 IN denotes with
T15610 6413-6417 NN denotes tail
T15611 6418-6425 VBN denotes wrapped
T15612 6426-6432 IN denotes around
T15613 6433-6436 DT denotes the
T15614 6437-6440 NN denotes bar
T15615 6440-6441 : denotes ;
T15616 6442-6443 CD denotes 5
T15617 6443-6445 , denotes ,
T15556 6445-6452 VBD denotes escaped
T15618 6453-6455 IN denotes to
T15619 6456-6459 CD denotes one
T15620 6460-6462 IN denotes of
T15621 6463-6466 DT denotes the
T15622 6467-6475 NNS denotes supports
T15623 6475-6476 . denotes .
T15705 6478-6487 NN denotes Footprint
T15706 6488-6492 NN denotes test
T15707 6492-6621 sentence denotes Black ink was applied to the hind paws of each mouse and the mice were placed in a narrow alley (9 × 25 × 10 cm) on white paper.
T15708 6493-6498 JJ denotes Black
T15709 6499-6502 NN denotes ink
T15711 6503-6506 VBD denotes was
T15710 6507-6514 VBN denotes applied
T15712 6515-6517 IN denotes to
T15713 6518-6521 DT denotes the
T15715 6522-6526 NN denotes hind
T15714 6527-6531 NNS denotes paws
T15716 6532-6534 IN denotes of
T15717 6535-6539 DT denotes each
T15718 6540-6545 NN denotes mouse
T15719 6546-6549 CC denotes and
T15720 6550-6553 DT denotes the
T15721 6554-6558 NNS denotes mice
T15723 6559-6563 VBD denotes were
T15722 6564-6570 VBN denotes placed
T15724 6571-6573 IN denotes in
T15725 6574-6575 DT denotes a
T15727 6576-6582 JJ denotes narrow
T15726 6583-6588 NN denotes alley
T15728 6589-6590 -LRB- denotes (
T15730 6590-6591 CD denotes 9
T15732 6592-6593 SYM denotes ×
T15733 6594-6596 CD denotes 25
T15734 6597-6598 SYM denotes ×
T15731 6599-6601 CD denotes 10
T15729 6602-6604 NN denotes cm
T15735 6604-6605 -RRB- denotes )
T15736 6606-6608 IN denotes on
T15737 6609-6614 JJ denotes white
T15738 6615-6620 NN denotes paper
T15739 6620-6621 . denotes .
T15740 6621-6667 sentence denotes Stride length and step width were calculated.
T15741 6622-6628 NN denotes Stride
T15742 6629-6635 NN denotes length
T15744 6636-6639 CC denotes and
T15745 6640-6644 NN denotes step
T15746 6645-6650 NN denotes width
T15747 6651-6655 VBD denotes were
T15743 6656-6666 VBN denotes calculated
T15748 6666-6667 . denotes .
T15949 6669-6679 NNS denotes Statistics
T15950 6679-6718 sentence denotes Data are expressed as the means ± SEM.
T15951 6680-6684 NNS denotes Data
T15953 6685-6688 VBP denotes are
T15952 6689-6698 VBN denotes expressed
T15954 6699-6701 IN denotes as
T15955 6702-6705 DT denotes the
T15957 6706-6711 NNS denotes means
T15958 6712-6713 SYM denotes ±
T15956 6714-6717 NN denotes SEM
T15959 6717-6718 . denotes .
T15960 6718-6830 sentence denotes Statistical analysis was conducted using the software package SAS 8.1 (SAS Institute Japan Ltd., Tokyo, Japan).
T15961 6719-6730 JJ denotes Statistical
T15962 6731-6739 NN denotes analysis
T15964 6740-6743 VBD denotes was
T15963 6744-6753 VBN denotes conducted
T15965 6754-6759 VBG denotes using
T15966 6760-6763 DT denotes the
T15968 6764-6772 NN denotes software
T15967 6773-6780 NN denotes package
T15969 6781-6784 NN denotes SAS
T15970 6785-6788 CD denotes 8.1
T15971 6789-6790 -LRB- denotes (
T15973 6790-6793 NNP denotes SAS
T15974 6794-6803 NNP denotes Institute
T15972 6804-6809 NNP denotes Japan
T15975 6810-6814 NNP denotes Ltd.
T15976 6814-6816 , denotes ,
T15977 6816-6821 NNP denotes Tokyo
T15978 6821-6823 , denotes ,
T15979 6823-6828 NNP denotes Japan
T15980 6828-6829 -RRB- denotes )
T15981 6829-6830 . denotes .
T15982 6830-7046 sentence denotes Statistical significance was determined by analysis of variance (ANOVA) followed by the Dunnett-type multiple comparison test, and p values of less than 0.05 were considered to be significant in the water maze task.
T15983 6831-6842 JJ denotes Statistical
T15984 6843-6855 NN denotes significance
T15986 6856-6859 VBD denotes was
T15985 6860-6870 VBN denotes determined
T15987 6871-6873 IN denotes by
T15988 6874-6882 NN denotes analysis
T15989 6883-6885 IN denotes of
T15990 6886-6894 NN denotes variance
T15991 6895-6896 -LRB- denotes (
T15992 6896-6901 NN denotes ANOVA
T15993 6901-6902 -RRB- denotes )
T15994 6903-6911 VBN denotes followed
T15995 6912-6914 IN denotes by
T15996 6915-6918 DT denotes the
T15998 6919-6926 NNP denotes Dunnett
T16000 6926-6927 HYPH denotes -
T15999 6927-6931 NN denotes type
T16001 6932-6940 JJ denotes multiple
T16002 6941-6951 NN denotes comparison
T15997 6952-6956 NN denotes test
T16003 6956-6958 , denotes ,
T16004 6958-6961 CC denotes and
T16005 6962-6963 NN denotes p
T16006 6964-6970 NNS denotes values
T16008 6971-6973 IN denotes of
T16009 6974-6978 JJR denotes less
T16011 6979-6983 IN denotes than
T16010 6984-6988 CD denotes 0.05
T16012 6989-6993 VBD denotes were
T16007 6994-7004 VBN denotes considered
T16013 7005-7007 TO denotes to
T16014 7008-7010 VB denotes be
T16015 7011-7022 JJ denotes significant
T16016 7023-7025 IN denotes in
T16017 7026-7029 DT denotes the
T16019 7030-7035 NN denotes water
T16020 7036-7040 NN denotes maze
T16018 7041-7045 NN denotes task
T16021 7045-7046 . denotes .
T16022 7046-7217 sentence denotes Behavioural data in the rotating rod test were analysed by one- or two-way ANOVA with repeated measures, and p values of less than 0.05 were considered to be significant.
T16023 7047-7058 JJ denotes Behavioural
T16024 7059-7063 NNS denotes data
T16026 7064-7066 IN denotes in
T16027 7067-7070 DT denotes the
T16029 7071-7079 VBG denotes rotating
T16030 7080-7083 NN denotes rod
T16028 7084-7088 NN denotes test
T16031 7089-7093 VBD denotes were
T16025 7094-7102 VBN denotes analysed
T16032 7103-7105 IN denotes by
T16033 7106-7109 CD denotes one
T16035 7109-7110 HYPH denotes -
T16036 7111-7113 CC denotes or
T16037 7114-7117 CD denotes two
T16038 7117-7118 HYPH denotes -
T16034 7118-7121 NN denotes way
T16039 7122-7127 NN denotes ANOVA
T16040 7128-7132 IN denotes with
T16041 7133-7141 VBN denotes repeated
T16042 7142-7150 NNS denotes measures
T16043 7150-7152 , denotes ,
T16044 7152-7155 CC denotes and
T16045 7156-7157 NN denotes p
T16046 7158-7164 NNS denotes values
T16048 7165-7167 IN denotes of
T16049 7168-7172 JJR denotes less
T16051 7173-7177 IN denotes than
T16050 7178-7182 CD denotes 0.05
T16052 7183-7187 VBD denotes were
T16047 7188-7198 VBN denotes considered
T16053 7199-7201 TO denotes to
T16054 7202-7204 VB denotes be
T16055 7205-7216 JJ denotes significant
T16056 7216-7217 . denotes .
T16221 7219-7233 NN denotes Histopathology
T16222 7233-7454 sentence denotes Sections of tissues including the brain, spinal cord, heart, lung, liver, kidney, spleen and testis from mice (+/+, +/- and -/-; n = 7, 7 and 7, respectively) were fixed in 10% buffered formalin and embedded in paraffin.
T16223 7234-7242 NNS denotes Sections
T16225 7243-7245 IN denotes of
T16226 7246-7253 NNS denotes tissues
T16227 7254-7263 VBG denotes including
T16228 7264-7267 DT denotes the
T16229 7268-7273 NN denotes brain
T16230 7273-7275 , denotes ,
T16231 7275-7281 JJ denotes spinal
T16232 7282-7286 NN denotes cord
T16233 7286-7288 , denotes ,
T16234 7288-7293 NN denotes heart
T16235 7293-7295 , denotes ,
T16236 7295-7299 NN denotes lung
T16237 7299-7301 , denotes ,
T16238 7301-7306 NN denotes liver
T16239 7306-7308 , denotes ,
T16240 7308-7314 NN denotes kidney
T16241 7314-7316 , denotes ,
T16242 7316-7322 NN denotes spleen
T16243 7323-7326 CC denotes and
T16244 7327-7333 NN denotes testis
T16245 7334-7338 IN denotes from
T16246 7339-7343 NNS denotes mice
T16247 7344-7345 -LRB- denotes (
T16249 7345-7346 SYM denotes +
T16250 7346-7347 HYPH denotes /
T16248 7347-7348 SYM denotes +
T16251 7348-7350 , denotes ,
T16252 7350-7351 SYM denotes +
T16254 7351-7352 HYPH denotes /
T16253 7352-7353 SYM denotes -
T16255 7354-7357 CC denotes and
T16256 7358-7359 SYM denotes -
T16258 7359-7360 HYPH denotes /
T16257 7360-7361 SYM denotes -
T16259 7361-7362 : denotes ;
T16260 7363-7364 NN denotes n
T16262 7365-7366 SYM denotes =
T16261 7367-7368 CD denotes 7
T16263 7368-7370 , denotes ,
T16264 7370-7371 CD denotes 7
T16265 7372-7375 CC denotes and
T16266 7376-7377 CD denotes 7
T16267 7377-7379 , denotes ,
T16268 7379-7391 RB denotes respectively
T16269 7391-7392 -RRB- denotes )
T16270 7393-7397 VBD denotes were
T16224 7398-7403 VBN denotes fixed
T16271 7404-7406 IN denotes in
T16272 7407-7409 CD denotes 10
T16273 7409-7410 NN denotes %
T16275 7411-7419 VBN denotes buffered
T16274 7420-7428 NN denotes formalin
T16276 7429-7432 CC denotes and
T16277 7433-7441 VBN denotes embedded
T16278 7442-7444 IN denotes in
T16279 7445-7453 NN denotes paraffin
T16280 7453-7454 . denotes .
T16281 7454-7535 sentence denotes Each paraffin section of thick 4 μm was stained with hematoxylin and eosin (HE).
T16282 7455-7459 DT denotes Each
T16284 7460-7468 NN denotes paraffin
T16283 7469-7476 NN denotes section
T16286 7477-7479 IN denotes of
T16287 7480-7485 JJ denotes thick
T16289 7486-7487 CD denotes 4
T16288 7488-7490 NN denotes μm
T16290 7491-7494 VBD denotes was
T16285 7495-7502 VBN denotes stained
T16291 7503-7507 IN denotes with
T16292 7508-7519 NN denotes hematoxylin
T16293 7520-7523 CC denotes and
T16294 7524-7529 NN denotes eosin
T16295 7530-7531 -LRB- denotes (
T16296 7531-7533 NN denotes HE
T16297 7533-7534 -RRB- denotes )
T16298 7534-7535 . denotes .
R3521 T11865 T11864 prep of,Construction
R3522 T11866 T11867 det an,vector
R3523 T11867 T11865 pobj vector,of
R3524 T11868 T11867 nmod Adam11,vector
R3525 T11869 T11867 nmod gene,vector
R3526 T11870 T11869 punct -,gene
R3527 T11871 T11869 amod targeting,gene
R3528 T11873 T11874 det The,fragment
R3529 T11874 T11880 nsubjpass fragment,amplified
R3530 T11875 T11876 nummod 13.7,kb
R3531 T11876 T11874 compound kb,fragment
R3532 T11877 T11876 punct -,kb
R3533 T11878 T11874 compound BamHI,fragment
R3534 T11879 T11874 compound DNA,fragment
R3535 T11881 T11874 acl covering,fragment
R3536 T11882 T11883 nmod exons,2
R3537 T11883 T11881 dobj 2,covering
R3538 T11884 T11885 punct –,20
R3539 T11885 T11883 prep 20,2
R3540 T11886 T11883 prep of,2
R3541 T11887 T11888 det the,gene
R3542 T11888 T11886 pobj gene,of
R3543 T11889 T11888 compound Adam11,gene
R3544 T11890 T11880 auxpass was,amplified
R3545 T11891 T11880 prep from,amplified
R3546 T11892 T11893 nmod C57BL,DNA
R3547 T11893 T11891 pobj DNA,from
R3548 T11894 T11892 punct /,C57BL
R3549 T11895 T11892 nummod 6,C57BL
R3550 T11896 T11893 amod genomic,DNA
R3551 T11897 T11880 prep by,amplified
R3552 T11898 T11899 det the,PCR
R3553 T11899 T11897 pobj PCR,by
R3554 T11900 T11899 acl using,PCR
R3555 T11901 T11902 compound Expand,mix
R3556 T11902 T11900 dobj mix,using
R3557 T11903 T11904 compound Hi,Fidelity
R3558 T11904 T11902 compound Fidelity,mix
R3559 T11905 T11904 punct -,Fidelity
R3560 T11906 T11902 compound polymerase,mix
R3561 T11907 T11908 punct (,KK
R3562 T11908 T11902 parataxis KK,mix
R3563 T11909 T11908 compound Roche,KK
R3564 T11910 T11908 compound Diagnostics,KK
R3565 T11911 T11908 punct ", ",KK
R3566 T11912 T11908 npadvmod Tokyo,KK
R3567 T11913 T11908 punct ", ",KK
R3568 T11914 T11908 npadvmod Japan,KK
R3569 T11915 T11908 punct ),KK
R3570 T11916 T11880 punct .,amplified
R3571 T11918 T11919 aux To,generate
R3572 T11919 T11920 advcl generate,inserted
R3573 T11921 T11922 det a,mutation
R3574 T11922 T11919 dobj mutation,generate
R3575 T11923 T11919 prep in,generate
R3576 T11924 T11925 det the,gene
R3577 T11925 T11923 pobj gene,in
R3578 T11926 T11925 compound mouse,gene
R3579 T11927 T11925 compound Adam11,gene
R3580 T11928 T11920 punct ", ",inserted
R3581 T11929 T11920 nsubj we,inserted
R3582 T11930 T11931 det a,cassette
R3583 T11931 T11920 dobj cassette,inserted
R3584 T11932 T11931 compound PGKneo,cassette
R3585 T11933 T11920 prep into,inserted
R3586 T11934 T11935 nmod exons,5
R3587 T11935 T11933 pobj 5,into
R3588 T11936 T11937 punct –,7
R3589 T11937 T11935 prep 7,5
R3590 T11938 T11935 prep of,5
R3591 T11939 T11940 det the,gene
R3592 T11940 T11938 pobj gene,of
R3593 T11941 T11940 compound Adam11,gene
R3594 T11942 T11920 punct .,inserted
R3595 T11944 T11945 det The,construct
R3596 T11945 T11948 nsubj construct,consisted
R3597 T11946 T11945 amod final,construct
R3598 T11947 T11945 compound targeting,construct
R3599 T11949 T11948 prep of,consisted
R3600 T11950 T11951 det a,fragment
R3601 T11951 T11949 pobj fragment,of
R3602 T11952 T11953 nummod 10.1,kb
R3603 T11953 T11951 nmod kb,fragment
R3604 T11954 T11953 punct -,kb
R3605 T11955 T11951 amod genomic,fragment
R3606 T11956 T11951 compound DNA,fragment
R3607 T11957 T11958 dep that,interrupted
R3608 T11958 T11951 relcl interrupted,fragment
R3609 T11959 T11958 auxpass was,interrupted
R3610 T11960 T11958 agent by,interrupted
R3611 T11961 T11962 det the,insertion
R3612 T11962 T11960 pobj insertion,by
R3613 T11963 T11962 prep of,insertion
R3614 T11964 T11965 det the,cassette
R3615 T11965 T11963 pobj cassette,of
R3616 T11966 T11965 compound PGKneo,cassette
R3617 T11967 T11958 cc and,interrupted
R3618 T11968 T11958 conj contained,interrupted
R3619 T11969 T11970 det a,cassette
R3620 T11970 T11968 dobj cassette,contained
R3621 T11971 T11972 compound MC1,TK
R3622 T11972 T11970 compound TK,cassette
R3623 T11973 T11972 punct /,TK
R3624 T11974 T11968 prep as,contained
R3625 T11975 T11976 det a,marker
R3626 T11976 T11974 pobj marker,as
R3627 T11977 T11978 amod negative,selection
R3628 T11978 T11976 compound selection,marker
R3629 T11979 T11976 prep against,marker
R3630 T11980 T11981 amod random,integration
R3631 T11981 T11979 pobj integration,against
R3632 T11982 T11983 punct [,39
R3633 T11983 T11968 parataxis 39,contained
R3634 T11984 T11983 punct ],39
R3635 T11985 T11948 punct .,consisted
R3640 T12310 T12309 prep of,Generation
R3641 T12311 T12312 npadvmod Adam11,deficient
R3642 T12312 T12313 amod deficient,mice
R3643 T12313 T12310 pobj mice,of
R3644 T12315 T12316 det The,vector
R3645 T12316 T12319 nsubjpass vector,electroporated
R3646 T12317 T12316 amod linearised,vector
R3647 T12318 T12316 compound targeting,vector
R3648 T12320 T12319 auxpass was,electroporated
R3649 T12321 T12319 prep into,electroporated
R3650 T12322 T12323 nmod TT2,cells
R3651 T12323 T12321 pobj cells,into
R3652 T12324 T12325 amod embryonic,stem
R3653 T12325 T12323 nmod stem,cells
R3654 T12326 T12325 punct (,stem
R3655 T12327 T12325 appos ES,stem
R3656 T12328 T12323 punct ),cells
R3657 T12329 T12330 punct [,40
R3658 T12330 T12319 parataxis 40,electroporated
R3659 T12331 T12330 punct ],40
R3660 T12332 T12319 punct .,electroporated
R3661 T12334 T12335 amod Homologous,recombinants
R3662 T12335 T12336 nsubjpass recombinants,selected
R3663 T12337 T12336 auxpass were,selected
R3664 T12338 T12336 prep by,selected
R3665 T12339 T12338 pobj PCR,by
R3666 T12340 T12336 advcl using,selected
R3667 T12341 T12342 det the,primers
R3668 T12342 T12340 dobj primers,using
R3669 T12343 T12342 amod following,primers
R3670 T12344 T12342 punct ", ",primers
R3671 T12345 T12342 appos AGN1,primers
R3672 T12346 T12345 punct : ,AGN1
R3673 T12347 T12345 appos 5,AGN1
R3674 T12348 T12347 punct ',5
R3675 T12349 T12347 punct -,5
R3676 T12350 T12347 appos TCGTGCTTTACGGTATCGCCGCTCCCGATT,5
R3677 T12351 T12347 punct -,5
R3678 T12352 T12347 nummod 3,5
R3679 T12353 T12347 punct ',5
R3680 T12354 T12345 prep in,AGN1
R3681 T12355 T12356 det the,cassette
R3682 T12356 T12354 pobj cassette,in
R3683 T12357 T12356 compound PGKneo,cassette
R3684 T12358 T12342 punct ", ",primers
R3685 T12359 T12342 cc and,primers
R3686 T12360 T12342 conj SGN033,primers
R3687 T12361 T12360 punct : ,SGN033
R3688 T12362 T12360 appos 5,SGN033
R3689 T12363 T12362 punct ',5
R3690 T12364 T12362 punct -,5
R3691 T12365 T12362 appos GGACCCGGAAGACATTTGCACGGT,5
R3692 T12366 T12362 punct -,5
R3693 T12367 T12362 nummod 3,5
R3694 T12368 T12362 punct ',5
R3695 T12369 T12360 prep outside,SGN033
R3696 T12370 T12371 det the,vector
R3697 T12371 T12369 pobj vector,outside
R3698 T12372 T12371 compound targeting,vector
R3699 T12373 T12336 punct .,selected
R3700 T12375 T12376 amod Homologous,DNA
R3701 T12376 T12378 nsubjpass DNA,amplified
R3702 T12377 T12376 amod recombined,DNA
R3703 T12379 T12378 auxpass was,amplified
R3704 T12380 T12378 advmod efficiently,amplified
R3705 T12381 T12378 agent by,amplified
R3706 T12382 T12383 nummod 35,cycles
R3707 T12383 T12381 pobj cycles,by
R3708 T12384 T12383 prep of,cycles
R3709 T12385 T12386 det the,steps
R3710 T12386 T12384 pobj steps,of
R3711 T12387 T12386 amod following,steps
R3712 T12388 T12386 compound amplification,steps
R3713 T12389 T12386 punct (,steps
R3714 T12390 T12391 nummod 94,°C
R3715 T12391 T12386 appos °C,steps
R3716 T12392 T12391 punct -,°C
R3717 T12393 T12394 nummod 30,sec
R3718 T12394 T12391 npadvmod sec,°C
R3719 T12395 T12391 cc and,°C
R3720 T12396 T12397 nummod 68,°C
R3721 T12397 T12391 conj °C,°C
R3722 T12398 T12397 punct -,°C
R3723 T12399 T12400 nummod 5,min
R3724 T12400 T12397 npadvmod min,°C
R3725 T12401 T12386 punct ),steps
R3726 T12402 T12378 punct .,amplified
R3727 T12404 T12405 advmod Correctly,targeted
R3728 T12405 T12406 amod targeted,clones
R3729 T12406 T12408 nsubjpass clones,injected
R3730 T12407 T12406 compound ES,clones
R3731 T12409 T12408 auxpass were,injected
R3732 T12410 T12408 prep into,injected
R3733 T12411 T12412 amod fertilised,eggs
R3734 T12412 T12410 pobj eggs,into
R3735 T12413 T12412 compound ICR,eggs
R3736 T12414 T12412 compound mouse,eggs
R3737 T12415 T12408 prep at,injected
R3738 T12416 T12417 det the,stage
R3739 T12417 T12415 pobj stage,at
R3740 T12418 T12419 nummod eight,cell
R3741 T12419 T12417 compound cell,stage
R3742 T12420 T12419 punct -,cell
R3743 T12421 T12408 punct .,injected
R3744 T12423 T12424 det The,chimeras
R3745 T12424 T12427 nsubjpass chimeras,mated
R3746 T12425 T12424 amod resulting,chimeras
R3747 T12426 T12424 amod male,chimeras
R3748 T12428 T12427 auxpass were,mated
R3749 T12429 T12427 prep with,mated
R3750 T12430 T12431 compound C57BL,6NCrj
R3751 T12431 T12433 compound 6NCrj,females
R3752 T12432 T12431 punct /,6NCrj
R3753 T12433 T12429 pobj females,with
R3754 T12434 T12435 punct (,Japan
R3755 T12435 T12427 parataxis Japan,mated
R3756 T12436 T12435 compound Charles,Japan
R3757 T12437 T12435 compound River,Japan
R3758 T12438 T12435 punct ", ",Japan
R3759 T12439 T12435 npadvmod Tokyo,Japan
R3760 T12440 T12435 punct ", ",Japan
R3761 T12441 T12435 npadvmod Japan,Japan
R3762 T12442 T12435 punct ),Japan
R3763 T12443 T12427 punct ", ",mated
R3764 T12444 T12427 cc and,mated
R3765 T12445 T12446 amod resulting,mice
R3766 T12446 T12456 nsubjpass mice,interbred
R3767 T12447 T12446 amod heterozygous,mice
R3768 T12448 T12446 punct (,mice
R3769 T12449 T12450 punct +,-
R3770 T12450 T12446 punct -,mice
R3771 T12451 T12450 punct /,-
R3772 T12452 T12446 punct ),mice
R3773 T12453 T12446 amod male,mice
R3774 T12454 T12453 cc and,male
R3775 T12455 T12453 conj female,male
R3776 T12456 T12427 conj interbred,mated
R3777 T12457 T12456 auxpass were,interbred
R3778 T12458 T12459 aux to,generate
R3779 T12459 T12456 advcl generate,interbred
R3780 T12460 T12461 amod homozygous,mice
R3781 T12461 T12459 dobj mice,generate
R3782 T12462 T12461 punct (,mice
R3783 T12463 T12464 punct -,-
R3784 T12464 T12461 punct -,mice
R3785 T12465 T12464 punct /,-
R3786 T12466 T12461 punct ),mice
R3787 T12467 T12456 punct .,interbred
R3788 T12588 T12589 compound Southern,blot
R3789 T12589 T12590 compound blot,analysis
R3790 T12592 T12593 nmod Mouse,DNA
R3791 T12593 T12595 nsubjpass DNA,extracted
R3792 T12594 T12593 amod genomic,DNA
R3793 T12596 T12593 acl used,DNA
R3794 T12597 T12596 prep for,used
R3795 T12598 T12599 compound Southern,blot
R3796 T12599 T12600 compound blot,analysis
R3797 T12600 T12597 pobj analysis,for
R3798 T12601 T12595 auxpass was,extracted
R3799 T12602 T12595 prep from,extracted
R3800 T12603 T12604 det the,liver
R3801 T12604 T12602 pobj liver,from
R3802 T12605 T12604 prep of,liver
R3803 T12606 T12607 det each,genotype
R3804 T12607 T12605 pobj genotype,of
R3805 T12608 T12595 punct .,extracted
R3806 T12610 T12611 npadvmod BamHI,digested
R3807 T12611 T12613 amod digested,DNA
R3808 T12612 T12611 punct -,digested
R3809 T12613 T12615 nsubjpass DNA,probed
R3810 T12614 T12613 amod genomic,DNA
R3811 T12616 T12615 auxpass was,probed
R3812 T12617 T12615 prep with,probed
R3813 T12618 T12619 det the,probe
R3814 T12619 T12617 pobj probe,with
R3815 T12620 T12619 punct [,probe
R3816 T12621 T12622 npadvmod 32P,labelled
R3817 T12622 T12619 amod labelled,probe
R3818 T12623 T12622 punct ],labelled
R3819 T12624 T12622 punct -,labelled
R3820 T12625 T12619 compound BE2K,probe
R3821 T12626 T12619 punct ", ",probe
R3822 T12627 T12628 dep which,was
R3823 T12628 T12619 relcl was,probe
R3824 T12629 T12630 det a,fragment
R3825 T12630 T12628 attr fragment,was
R3826 T12631 T12632 nummod 2.0,kb
R3827 T12632 T12630 nmod kb,fragment
R3828 T12633 T12632 punct -,kb
R3829 T12634 T12635 nmod BamHI,EcoRI
R3830 T12635 T12630 nmod EcoRI,fragment
R3831 T12636 T12635 punct –,EcoRI
R3832 T12637 T12630 amod genomic,fragment
R3833 T12638 T12630 acl located,fragment
R3834 T12639 T12638 prep in,located
R3835 T12640 T12639 pobj intron,in
R3836 T12641 T12640 nummod 2,intron
R3837 T12642 T12615 punct .,probed
R3838 T13011 T13012 compound Western,blot
R3839 T13012 T13013 compound blot,analysis
R3840 T13015 T13016 det The,absence
R3841 T13016 T13017 nsubjpass absence,confirmed
R3842 T13018 T13016 prep of,absence
R3843 T13019 T13020 compound ADAM11,protein
R3844 T13020 T13018 pobj protein,of
R3845 T13021 T13016 prep in,absence
R3846 T13022 T13023 det the,mice
R3847 T13023 T13021 pobj mice,in
R3848 T13024 T13023 punct (,mice
R3849 T13025 T13026 punct -,-
R3850 T13026 T13023 punct -,mice
R3851 T13027 T13026 punct /,-
R3852 T13028 T13023 punct ),mice
R3853 T13029 T13017 auxpass was,confirmed
R3854 T13030 T13017 agent by,confirmed
R3855 T13031 T13032 compound Western,blot
R3856 T13032 T13033 compound blot,analysis
R3857 T13033 T13030 pobj analysis,by
R3858 T13034 T13017 punct .,confirmed
R3859 T13036 T13037 aux To,be
R3860 T13037 T13038 advcl be,isolated
R3861 T13039 T13037 acomp brief,be
R3862 T13040 T13038 punct ", ",isolated
R3863 T13041 T13042 det the,cerebellum
R3864 T13042 T13038 nsubjpass cerebellum,isolated
R3865 T13043 T13038 auxpass was,isolated
R3866 T13044 T13038 prep from,isolated
R3867 T13045 T13046 det a,mouse
R3868 T13046 T13044 pobj mouse,from
R3869 T13047 T13048 nummod six,month
R3870 T13048 T13049 npadvmod month,old
R3871 T13049 T13046 amod old,mouse
R3872 T13050 T13049 punct -,old
R3873 T13051 T13046 prep of,mouse
R3874 T13052 T13053 det each,genotype
R3875 T13053 T13051 pobj genotype,of
R3876 T13054 T13038 cc and,isolated
R3877 T13055 T13038 conj homogenised,isolated
R3878 T13056 T13055 prep in,homogenised
R3879 T13057 T13058 compound cell,lysis
R3880 T13058 T13059 compound lysis,buffer
R3881 T13059 T13056 pobj buffer,in
R3882 T13060 T13061 punct (,cocktail
R3883 T13061 T13055 parataxis cocktail,homogenised
R3884 T13062 T13063 nummod 50,mM
R3885 T13063 T13064 compound mM,HCl
R3886 T13064 T13061 dep HCl,cocktail
R3887 T13065 T13064 compound Tris,HCl
R3888 T13066 T13064 punct -,HCl
R3889 T13067 T13061 punct ", ",cocktail
R3890 T13068 T13061 dep pH,cocktail
R3891 T13069 T13068 nummod 7.5,pH
R3892 T13070 T13061 punct ", ",cocktail
R3893 T13071 T13072 nummod 100,mM
R3894 T13072 T13073 compound mM,NaCl
R3895 T13073 T13061 dep NaCl,cocktail
R3896 T13074 T13061 punct ", ",cocktail
R3897 T13075 T13076 nummod 1,%
R3898 T13076 T13077 compound %,NP
R3899 T13077 T13061 dep NP,cocktail
R3900 T13078 T13077 punct -,NP
R3901 T13079 T13077 nummod 40,NP
R3902 T13080 T13061 punct ", ",cocktail
R3903 T13081 T13082 compound protease,inhibitor
R3904 T13082 T13061 compound inhibitor,cocktail
R3905 T13083 T13084 punct [,Diagnostics
R3906 T13084 T13061 parataxis Diagnostics,cocktail
R3907 T13085 T13084 compound Roche,Diagnostics
R3908 T13086 T13084 punct ],Diagnostics
R3909 T13087 T13061 punct ),cocktail
R3910 T13088 T13038 punct .,isolated
R3911 T13090 T13091 prep After,concentrated
R3912 T13092 T13090 pobj removal,After
R3913 T13093 T13092 prep of,removal
R3914 T13094 T13095 compound cell,debris
R3915 T13095 T13093 pobj debris,of
R3916 T13096 T13092 prep by,removal
R3917 T13097 T13096 pobj centrifugation,by
R3918 T13098 T13091 punct ", ",concentrated
R3919 T13099 T13100 det the,proteins
R3920 T13100 T13091 nsubjpass proteins,concentrated
R3921 T13101 T13100 amod glycosylated,proteins
R3922 T13102 T13100 prep in,proteins
R3923 T13103 T13104 det the,supernatant
R3924 T13104 T13102 pobj supernatant,in
R3925 T13105 T13091 auxpass were,concentrated
R3926 T13106 T13091 prep by,concentrated
R3927 T13107 T13108 det the,chromatography
R3928 T13108 T13106 pobj chromatography,by
R3929 T13109 T13108 compound affinity,chromatography
R3930 T13110 T13091 advcl using,concentrated
R3931 T13111 T13112 compound Con,4B
R3932 T13112 T13110 dobj 4B,using
R3933 T13113 T13112 compound A,4B
R3934 T13114 T13112 compound Sepharose,4B
R3935 T13115 T13116 punct (,Corp.
R3936 T13116 T13110 parataxis Corp.,using
R3937 T13117 T13116 compound Amersham,Corp.
R3938 T13118 T13116 compound Biosciences,Corp.
R3939 T13119 T13116 punct ),Corp.
R3940 T13120 T13091 punct .,concentrated
R3941 T13122 T13123 det Each,sample
R3942 T13123 T13124 nsubjpass sample,separated
R3943 T13125 T13124 auxpass was,separated
R3944 T13126 T13124 prep on,separated
R3945 T13127 T13128 nummod 10,%
R3946 T13128 T13129 compound %,PAGE
R3947 T13129 T13126 pobj PAGE,on
R3948 T13130 T13129 compound SDS,PAGE
R3949 T13131 T13129 punct -,PAGE
R3950 T13132 T13124 punct ", ",separated
R3951 T13133 T13124 cc and,separated
R3952 T13134 T13124 conj transferred,separated
R3953 T13135 T13134 prep to,transferred
R3954 T13136 T13137 det a,membrane
R3955 T13137 T13135 pobj membrane,to
R3956 T13138 T13137 compound PVDF,membrane
R3957 T13139 T13124 punct .,separated
R3958 T13141 T13142 det The,blot
R3959 T13142 T13143 nsubjpass blot,incubated
R3960 T13144 T13143 auxpass was,incubated
R3961 T13145 T13143 advmod then,incubated
R3962 T13146 T13143 prep with,incubated
R3963 T13147 T13148 amod monoclonal,antibody
R3964 T13148 T13146 pobj antibody,with
R3965 T13149 T13148 prep against,antibody
R3966 T13150 T13151 det the,ADAM11
R3967 T13151 T13149 pobj ADAM11,against
R3968 T13152 T13153 punct (,Patent
R3969 T13153 T13151 parataxis Patent,ADAM11
R3970 T13154 T13155 compound U.,S.
R3971 T13155 T13153 compound S.,Patent
R3972 T13156 T13153 nummod "5,631,351",Patent
R3973 T13157 T13153 punct ),Patent
R3974 T13158 T13148 prep at,antibody
R3975 T13159 T13160 nummod 1,μg
R3976 T13160 T13158 pobj μg,at
R3977 T13161 T13162 punct /,ml
R3978 T13162 T13160 prep ml,μg
R3979 T13163 T13143 punct .,incubated
R3980 T13165 T13166 amod Bound,antibodies
R3981 T13166 T13167 nsubjpass antibodies,visualised
R3982 T13168 T13167 auxpass were,visualised
R3983 T13169 T13167 prep with,visualised
R3984 T13170 T13171 compound horseradish,peroxidase
R3985 T13171 T13172 npadvmod peroxidase,labelled
R3986 T13172 T13174 amod labelled,antibody
R3987 T13173 T13172 punct -,labelled
R3988 T13174 T13169 pobj antibody,with
R3989 T13175 T13174 amod second,antibody
R3990 T13176 T13174 cc and,antibody
R3991 T13177 T13178 det an,system
R3992 T13178 T13174 conj system,antibody
R3993 T13179 T13180 compound ECL,plus
R3994 T13180 T13178 compound plus,system
R3995 T13181 T13180 punct -,plus
R3996 T13182 T13183 compound chemiluminescence,detection
R3997 T13183 T13178 compound detection,system
R3998 T13184 T13185 punct (,Corp.
R3999 T13185 T13167 parataxis Corp.,visualised
R4000 T13186 T13185 compound Amersham,Corp.
R4001 T13187 T13185 compound Biosciences,Corp.
R4002 T13188 T13185 punct ),Corp.
R4003 T13189 T13167 punct .,visualised
R4004 T13416 T13417 amod Behavioural,analysis
R4005 T13419 T13420 det All,procedures
R4006 T13420 T13422 nsubj procedures,conformed
R4007 T13421 T13420 compound animal,procedures
R4008 T13423 T13422 prep to,conformed
R4009 T13424 T13425 det the,regulations
R4010 T13425 T13423 pobj regulations,to
R4011 T13426 T13425 amod Japanese,regulations
R4012 T13427 T13425 prep on,regulations
R4013 T13428 T13429 compound animal,care
R4014 T13429 T13427 pobj care,on
R4015 T13430 T13429 cc and,care
R4016 T13431 T13429 conj use,care
R4017 T13432 T13425 punct ", ",regulations
R4018 T13433 T13425 prep following,regulations
R4019 T13434 T13435 det the,Guideline
R4020 T13435 T13433 pobj Guideline,following
R4021 T13436 T13435 prep for,Guideline
R4022 T13437 T13438 compound Animal,Experimentation
R4023 T13438 T13436 pobj Experimentation,for
R4024 T13439 T13435 prep of,Guideline
R4025 T13440 T13441 det the,Association
R4026 T13441 T13439 pobj Association,of
R4027 T13442 T13441 compound Japanese,Association
R4028 T13443 T13441 prep for,Association
R4029 T13444 T13443 pobj Laboratory,for
R4030 T13445 T13444 prep of,Laboratory
R4031 T13446 T13447 compound Animal,Science
R4032 T13447 T13445 pobj Science,of
R4033 T13448 T13422 punct ", ",conformed
R4034 T13449 T13422 cc and,conformed
R4035 T13450 T13451 auxpass were,approved
R4036 T13451 T13422 conj approved,conformed
R4037 T13452 T13451 agent by,approved
R4038 T13453 T13454 det the,Committee
R4039 T13454 T13452 pobj Committee,by
R4040 T13455 T13456 nmod Animal,Care
R4041 T13456 T13454 nmod Care,Committee
R4042 T13457 T13456 cc and,Care
R4043 T13458 T13456 conj Use,Care
R4044 T13459 T13454 prep of,Committee
R4045 T13460 T13461 compound Eisai,Co.
R4046 T13461 T13459 pobj Co.,of
R4047 T13462 T13461 punct ", ",Co.
R4048 T13463 T13461 amod Ltd,Co.
R4049 T13464 T13422 punct .,conformed
R4050 T13466 T13467 prep For,used
R4051 T13468 T13469 det the,experiments
R4052 T13469 T13466 pobj experiments,For
R4053 T13470 T13469 amod present,experiments
R4054 T13471 T13467 punct ", ",used
R4055 T13472 T13473 npadvmod ADAM11,deficient
R4056 T13473 T13475 amod deficient,mice
R4057 T13474 T13473 punct -,deficient
R4058 T13475 T13467 nsubjpass mice,used
R4059 T13476 T13475 amod male,mice
R4060 T13477 T13478 punct (,generations
R4061 T13478 T13475 parataxis generations,mice
R4062 T13479 T13478 amod backcrossed,generations
R4063 T13480 T13478 prep into,generations
R4064 T13481 T13482 compound C57BL,6NCrj
R4065 T13482 T13480 pobj 6NCrj,into
R4066 T13483 T13482 punct /,6NCrj
R4067 T13484 T13478 punct ", ",generations
R4068 T13485 T13486 nsubj n,14
R4069 T13486 T13478 ccomp 14,generations
R4070 T13487 T13486 punct =,14
R4071 T13488 T13478 punct ),generations
R4072 T13489 T13467 auxpass were,used
R4073 T13490 T13467 prep in,used
R4074 T13491 T13492 det all,analyses
R4075 T13492 T13490 pobj analyses,in
R4076 T13493 T13492 amod behavioural,analyses
R4077 T13494 T13495 punct (,weeks
R4078 T13495 T13467 parataxis weeks,used
R4079 T13496 T13497 quantmod 22,24
R4080 T13497 T13495 nummod 24,weeks
R4081 T13498 T13497 punct –,24
R4082 T13499 T13495 prep of,weeks
R4083 T13500 T13499 pobj age,of
R4084 T13501 T13495 punct ),weeks
R4085 T13502 T13467 punct .,used
R4086 T13504 T13505 nsubjpass All,conducted
R4087 T13506 T13504 prep of,All
R4088 T13507 T13508 det the,studies
R4089 T13508 T13506 pobj studies,of
R4090 T13509 T13508 amod behavioural,studies
R4091 T13510 T13505 auxpass were,conducted
R4092 T13511 T13505 prep between,conducted
R4093 T13512 T13513 nummod 10,00
R4094 T13513 T13511 pobj 00,between
R4095 T13514 T13513 punct :,00
R4096 T13515 T13513 cc and,00
R4097 T13516 T13517 nummod 16,00
R4098 T13517 T13513 conj 00,00
R4099 T13518 T13517 punct :,00
R4100 T13519 T13505 agent by,conducted
R4101 T13520 T13521 det a,experimenter
R4102 T13521 T13519 pobj experimenter,by
R4103 T13522 T13523 advmod well,trained
R4104 T13523 T13521 amod trained,experimenter
R4105 T13524 T13523 punct -,trained
R4106 T13525 T13521 amod blind,experimenter
R4107 T13526 T13525 prep to,blind
R4108 T13527 T13528 det the,genotypes
R4109 T13528 T13526 pobj genotypes,to
R4110 T13529 T13528 compound mouse,genotypes
R4111 T13530 T13505 punct .,conducted
R4112 T13661 T13662 amod Spontaneous,activity
R4113 T13663 T13662 compound motor,activity
R4114 T13665 T13666 compound Locomotor,activity
R4115 T13666 T13667 nsubjpass activity,measured
R4116 T13668 T13666 prep in,activity
R4117 T13669 T13670 det a,environment
R4118 T13670 T13668 pobj environment,in
R4119 T13671 T13670 amod novel,environment
R4120 T13672 T13667 auxpass was,measured
R4121 T13673 T13667 prep by,measured
R4122 T13674 T13673 pcomp introducing,by
R4123 T13675 T13674 dobj mice,introducing
R4124 T13676 T13677 punct (,+
R4125 T13677 T13674 punct +,introducing
R4126 T13678 T13677 punct +,+
R4127 T13679 T13677 punct /,+
R4128 T13680 T13677 punct ", ",+
R4129 T13681 T13682 punct +,-
R4130 T13682 T13677 conj -,+
R4131 T13683 T13682 punct /,-
R4132 T13684 T13682 cc and,-
R4133 T13685 T13686 punct -,-
R4134 T13686 T13682 conj -,-
R4135 T13687 T13686 punct /,-
R4136 T13688 T13677 punct ;,+
R4137 T13689 T13690 nsubj n,8
R4138 T13690 T13677 ccomp 8,+
R4139 T13691 T13690 punct =,8
R4140 T13692 T13690 punct ", ",8
R4141 T13693 T13690 conj 8,8
R4142 T13694 T13693 cc and,8
R4143 T13695 T13693 conj 8,8
R4144 T13696 T13690 punct ", ",8
R4145 T13697 T13690 advmod respectively,8
R4146 T13698 T13677 punct ),+
R4147 T13699 T13667 prep for,measured
R4148 T13700 T13701 nummod 90,min
R4149 T13701 T13699 pobj min,for
R4150 T13702 T13667 prep in,measured
R4151 T13703 T13704 det a,box
R4152 T13704 T13702 pobj box,in
R4153 T13705 T13704 compound Plexiglas,box
R4154 T13706 T13707 punct (,cm
R4155 T13707 T13704 parataxis cm,box
R4156 T13708 T13707 nummod 30,cm
R4157 T13709 T13710 punct ×,20
R4158 T13710 T13708 prep 20,30
R4159 T13711 T13712 punct ×,13
R4160 T13712 T13708 prep 13,30
R4161 T13713 T13707 punct ),cm
R4162 T13714 T13667 advcl using,measured
R4163 T13715 T13716 det a,equipment
R4164 T13716 T13714 dobj equipment,using
R4165 T13717 T13716 compound VERSAMAX,equipment
R4166 T13718 T13716 cc and,equipment
R4167 T13719 T13716 conj software,equipment
R4168 T13720 T13721 punct (,Accuscan
R4169 T13721 T13716 parataxis Accuscan,equipment
R4170 T13722 T13721 punct ", ",Accuscan
R4171 T13723 T13721 npadvmod Columbus,Accuscan
R4172 T13724 T13721 punct ", ",Accuscan
R4173 T13725 T13721 npadvmod OH,Accuscan
R4174 T13726 T13721 punct ", ",Accuscan
R4175 T13727 T13721 npadvmod USA,Accuscan
R4176 T13728 T13721 punct ),Accuscan
R4177 T13729 T13667 punct .,measured
R4178 T14364 T14365 compound Water,maze
R4179 T14365 T14366 compound maze,task
R4180 T14368 T14369 amod Spatial,learning
R4181 T14369 T14370 nsubjpass learning,assessed
R4182 T14371 T14370 auxpass was,assessed
R4183 T14372 T14370 prep by,assessed
R4184 T14373 T14374 nummod three,variants
R4185 T14374 T14372 pobj variants,by
R4186 T14375 T14374 prep of,variants
R4187 T14376 T14377 det the,task
R4188 T14377 T14375 pobj task,of
R4189 T14378 T14377 compound Morris,task
R4190 T14379 T14380 compound water,maze
R4191 T14380 T14377 compound maze,task
R4192 T14381 T14382 punct [,27
R4193 T14382 T14377 parataxis 27,task
R4194 T14383 T14382 punct ],27
R4195 T14384 T14374 acl adapted,variants
R4196 T14385 T14384 prep for,adapted
R4197 T14386 T14385 pobj mice,for
R4198 T14387 T14370 punct .,assessed
R4199 T14389 T14390 amod Hidden,task
R4200 T14390 T14392 dep task,divided
R4201 T14391 T14390 compound platform,task
R4202 T14393 T14392 punct : ,divided
R4203 T14394 T14395 det The,pool
R4204 T14395 T14392 nsubjpass pool,divided
R4205 T14396 T14392 auxpass was,divided
R4206 T14397 T14392 prep into,divided
R4207 T14398 T14399 nummod four,quadrants
R4208 T14399 T14397 pobj quadrants,into
R4209 T14400 T14401 mark with,at
R4210 T14401 T14392 advcl at,divided
R4211 T14402 T14403 nummod four,locations
R4212 T14403 T14401 nsubj locations,at
R4213 T14404 T14403 compound starting,locations
R4214 T14405 T14403 punct ", ",locations
R4215 T14406 T14403 acl called,locations
R4216 T14407 T14406 oprd north,called
R4217 T14408 T14407 punct ", ",north
R4218 T14409 T14407 conj east,north
R4219 T14410 T14409 punct ", ",east
R4220 T14411 T14409 conj south,east
R4221 T14412 T14411 cc and,south
R4222 T14413 T14411 conj west,south
R4223 T14414 T14401 punct ", ",at
R4224 T14415 T14416 amod equal,distances
R4225 T14416 T14401 pobj distances,at
R4226 T14417 T14416 prep to,distances
R4227 T14418 T14419 det the,rim
R4228 T14419 T14417 pobj rim,to
R4229 T14420 T14392 punct .,divided
R4230 T14422 T14423 det A,platform
R4231 T14423 T14428 nsubjpass platform,submerged
R4232 T14424 T14423 amod circular,platform
R4233 T14425 T14423 punct ", ",platform
R4234 T14426 T14423 amod transparent,platform
R4235 T14427 T14423 amod acrylic,platform
R4236 T14429 T14430 punct (,cm
R4237 T14430 T14423 parataxis cm,platform
R4238 T14431 T14430 nmod diameter,cm
R4239 T14432 T14430 nummod 8,cm
R4240 T14433 T14430 punct ),cm
R4241 T14434 T14428 auxpass was,submerged
R4242 T14435 T14436 nummod 1,cm
R4243 T14436 T14437 npadvmod cm,below
R4244 T14437 T14428 prep below,submerged
R4245 T14438 T14439 det the,surface
R4246 T14439 T14437 pobj surface,below
R4247 T14440 T14439 prep of,surface
R4248 T14441 T14442 det the,water
R4249 T14442 T14440 pobj water,of
R4250 T14443 T14428 prep in,submerged
R4251 T14444 T14445 det the,centre
R4252 T14445 T14443 pobj centre,in
R4253 T14446 T14445 prep of,centre
R4254 T14447 T14448 det the,quadrant
R4255 T14448 T14446 pobj quadrant,of
R4256 T14449 T14448 compound southeast,quadrant
R4257 T14450 T14428 prep for,submerged
R4258 T14451 T14452 det each,trial
R4259 T14452 T14450 pobj trial,for
R4260 T14453 T14452 prep of,trial
R4261 T14454 T14455 det this,task
R4262 T14455 T14453 pobj task,of
R4263 T14456 T14428 punct .,submerged
R4264 T14458 T14459 det Each,mouse
R4265 T14459 T14460 nsubjpass mouse,allowed
R4266 T14461 T14460 auxpass was,allowed
R4267 T14462 T14463 aux to,swim
R4268 T14463 T14460 xcomp swim,allowed
R4269 T14464 T14463 prep for,swim
R4270 T14465 T14466 nummod 60,sec
R4271 T14466 T14464 pobj sec,for
R4272 T14467 T14460 cc and,allowed
R4273 T14468 T14469 det the,time
R4274 T14469 T14470 nsubjpass time,recorded
R4275 T14470 T14460 conj recorded,allowed
R4276 T14471 T14469 acl required,time
R4277 T14472 T14473 aux to,reach
R4278 T14473 T14471 xcomp reach,required
R4279 T14474 T14475 det the,platform
R4280 T14475 T14473 dobj platform,reach
R4281 T14476 T14469 punct (,time
R4282 T14477 T14478 compound escape,latency
R4283 T14478 T14469 appos latency,time
R4284 T14479 T14469 punct ),time
R4285 T14480 T14470 auxpass was,recorded
R4286 T14481 T14470 prep in,recorded
R4287 T14482 T14483 det each,trial
R4288 T14483 T14481 pobj trial,in
R4289 T14484 T14470 punct .,recorded
R4290 T14486 T14487 prep In,consisted
R4291 T14488 T14486 amod total,In
R4292 T14489 T14490 det this,task
R4293 T14490 T14487 nsubj task,consisted
R4294 T14491 T14487 prep of,consisted
R4295 T14492 T14493 nummod four,trials
R4296 T14493 T14491 pobj trials,of
R4297 T14494 T14493 prep per,trials
R4298 T14495 T14494 pobj day,per
R4299 T14496 T14487 prep over,consisted
R4300 T14497 T14498 nummod 9,days
R4301 T14498 T14496 pobj days,over
R4302 T14499 T14500 punct (,intervals
R4303 T14500 T14498 parataxis intervals,days
R4304 T14501 T14502 nummod 1,min
R4305 T14502 T14500 nmod min,intervals
R4306 T14503 T14500 amod inter-trial,intervals
R4307 T14504 T14500 punct ),intervals
R4308 T14505 T14487 punct .,consisted
R4309 T14507 T14508 det Each,trial
R4310 T14508 T14509 nsubjpass trial,initiated
R4311 T14510 T14509 auxpass was,initiated
R4312 T14511 T14509 agent by,initiated
R4313 T14512 T14513 advmod randomly,placing
R4314 T14513 T14511 pcomp placing,by
R4315 T14514 T14515 det an,animal
R4316 T14515 T14513 dobj animal,placing
R4317 T14516 T14513 prep in,placing
R4318 T14517 T14516 pobj one,in
R4319 T14518 T14517 prep of,one
R4320 T14519 T14520 det the,locations
R4321 T14520 T14518 pobj locations,of
R4322 T14521 T14520 nummod four,locations
R4323 T14522 T14520 compound starting,locations
R4324 T14523 T14509 punct .,initiated
R4325 T14525 T14526 compound Probe,trial
R4326 T14526 T14527 dep trial,carried
R4327 T14528 T14527 punct : ,carried
R4328 T14529 T14530 det A,trial
R4329 T14530 T14527 nsubjpass trial,carried
R4330 T14531 T14530 amod single,trial
R4331 T14532 T14530 compound probe,trial
R4332 T14533 T14527 auxpass was,carried
R4333 T14534 T14527 prt out,carried
R4334 T14535 T14536 mark after,completed
R4335 T14536 T14527 advcl completed,carried
R4336 T14537 T14538 det the,task
R4337 T14538 T14536 nsubjpass task,completed
R4338 T14539 T14540 amod hidden,platform
R4339 T14540 T14538 compound platform,task
R4340 T14541 T14536 aux had,completed
R4341 T14542 T14536 auxpass been,completed
R4342 T14543 T14527 punct .,carried
R4343 T14545 T14546 prep In,removed
R4344 T14547 T14548 det this,trial
R4345 T14548 T14545 pobj trial,In
R4346 T14549 T14546 punct ", ",removed
R4347 T14550 T14551 det the,platform
R4348 T14551 T14546 nsubjpass platform,removed
R4349 T14552 T14546 auxpass was,removed
R4350 T14553 T14546 cc and,removed
R4351 T14554 T14555 det the,movement
R4352 T14555 T14556 nsubjpass movement,monitored
R4353 T14556 T14546 conj monitored,removed
R4354 T14557 T14555 prep of,movement
R4355 T14558 T14559 det each,mouse
R4356 T14559 T14557 pobj mouse,of
R4357 T14560 T14556 auxpass was,monitored
R4358 T14561 T14556 advcl using,monitored
R4359 T14562 T14563 det a,system
R4360 T14563 T14561 dobj system,using
R4361 T14564 T14565 npadvmod computer,based
R4362 T14565 T14563 amod based,system
R4363 T14566 T14565 punct -,based
R4364 T14567 T14568 compound video,tracking
R4365 T14568 T14563 compound tracking,system
R4366 T14569 T14570 punct (,Co.
R4367 T14570 T14561 parataxis Co.,using
R4368 T14571 T14570 dep BTA,Co.
R4369 T14572 T14571 punct -,BTA
R4370 T14573 T14571 nummod 2,BTA
R4371 T14574 T14570 punct ;,Co.
R4372 T14575 T14570 compound Muromachi,Co.
R4373 T14576 T14570 compound Kikai,Co.
R4374 T14577 T14570 punct ", ",Co.
R4375 T14578 T14570 amod Ltd.,Co.
R4376 T14579 T14570 punct ", ",Co.
R4377 T14580 T14570 npadvmod Tokyo,Co.
R4378 T14581 T14570 punct ", ",Co.
R4379 T14582 T14570 npadvmod Japan,Co.
R4380 T14583 T14570 punct ),Co.
R4381 T14584 T14546 punct .,removed
R4382 T14586 T14587 det Each,mouse
R4383 T14587 T14588 nsubjpass mouse,placed
R4384 T14589 T14588 auxpass was,placed
R4385 T14590 T14588 prep into,placed
R4386 T14591 T14592 det the,quadrant
R4387 T14592 T14590 pobj quadrant,into
R4388 T14593 T14592 compound northwest,quadrant
R4389 T14594 T14592 prep of,quadrant
R4390 T14595 T14596 det the,pool
R4391 T14596 T14594 pobj pool,of
R4392 T14597 T14588 cc and,placed
R4393 T14598 T14588 conj allowed,placed
R4394 T14599 T14600 aux to,swim
R4395 T14600 T14598 xcomp swim,allowed
R4396 T14601 T14600 prep for,swim
R4397 T14602 T14603 nummod 60,sec
R4398 T14603 T14601 pobj sec,for
R4399 T14604 T14588 punct .,placed
R4400 T14606 T14607 compound Swim,path
R4401 T14607 T14608 compound path,length
R4402 T14608 T14609 nsubjpass length,calculated
R4403 T14610 T14608 cc and,length
R4404 T14611 T14612 det the,time
R4405 T14612 T14608 conj time,length
R4406 T14613 T14612 acl spent,time
R4407 T14614 T14613 prep in,spent
R4408 T14615 T14616 det the,quadrant
R4409 T14616 T14614 pobj quadrant,in
R4410 T14617 T14616 amod trained,quadrant
R4411 T14618 T14616 punct (,quadrant
R4412 T14619 T14616 nmod southeast,quadrant
R4413 T14620 T14616 punct ),quadrant
R4414 T14621 T14609 auxpass were,calculated
R4415 T14622 T14609 punct .,calculated
R4416 T14624 T14625 amod Visible,task
R4417 T14625 T14627 dep task,made
R4418 T14626 T14625 compound platform,task
R4419 T14628 T14627 punct : ,made
R4420 T14629 T14627 prep In,made
R4421 T14630 T14631 det this,task
R4422 T14631 T14629 pobj task,In
R4423 T14632 T14627 punct ", ",made
R4424 T14633 T14634 det the,platform
R4425 T14634 T14627 nsubjpass platform,made
R4426 T14635 T14634 amod circular,platform
R4427 T14636 T14627 auxpass was,made
R4428 T14637 T14627 oprd visible,made
R4429 T14638 T14627 prep by,made
R4430 T14639 T14638 pcomp attaching,by
R4431 T14640 T14641 det a,board
R4432 T14641 T14639 dobj board,attaching
R4433 T14642 T14641 amod black,board
R4434 T14643 T14639 prep to,attaching
R4435 T14644 T14643 pobj it,to
R4436 T14645 T14627 cc and,made
R4437 T14646 T14647 det the,mouse
R4438 T14647 T14648 nsubjpass mouse,required
R4439 T14648 T14627 conj required,made
R4440 T14649 T14648 auxpass was,required
R4441 T14650 T14651 aux to,locate
R4442 T14651 T14648 advcl locate,required
R4443 T14652 T14653 det the,platform
R4444 T14653 T14651 dobj platform,locate
R4445 T14654 T14653 amod visible,platform
R4446 T14655 T14627 punct .,made
R4447 T14657 T14658 det The,mouse
R4448 T14658 T14659 nsubjpass mouse,allowed
R4449 T14660 T14659 auxpass was,allowed
R4450 T14661 T14662 aux to,swim
R4451 T14662 T14659 xcomp swim,allowed
R4452 T14663 T14662 prep for,swim
R4453 T14664 T14665 nummod 60,sec
R4454 T14665 T14663 pobj sec,for
R4455 T14666 T14659 cc and,allowed
R4456 T14667 T14668 det this,task
R4457 T14668 T14669 nsubj task,consisted
R4458 T14669 T14659 conj consisted,allowed
R4459 T14670 T14669 prep of,consisted
R4460 T14671 T14672 nummod four,trials
R4461 T14672 T14670 pobj trials,of
R4462 T14673 T14672 prep per,trials
R4463 T14674 T14673 pobj day,per
R4464 T14675 T14672 prep for,trials
R4465 T14676 T14677 nummod three,days
R4466 T14677 T14675 pobj days,for
R4467 T14678 T14677 amod consecutive,days
R4468 T14679 T14680 punct (,intervals
R4469 T14680 T14672 parataxis intervals,trials
R4470 T14681 T14682 nummod 1,min
R4471 T14682 T14680 nmod min,intervals
R4472 T14683 T14680 amod inter-trial,intervals
R4473 T14684 T14680 punct ),intervals
R4474 T14685 T14669 punct .,consisted
R4475 T14687 T14688 det Each,trial
R4476 T14688 T14689 nsubjpass trial,initiated
R4477 T14690 T14689 auxpass was,initiated
R4478 T14691 T14689 agent by,initiated
R4479 T14692 T14693 advmod randomly,placing
R4480 T14693 T14691 pcomp placing,by
R4481 T14694 T14695 det an,animal
R4482 T14695 T14693 dobj animal,placing
R4483 T14696 T14693 prep in,placing
R4484 T14697 T14696 pobj one,in
R4485 T14698 T14697 prep of,one
R4486 T14699 T14700 det the,locations
R4487 T14700 T14698 pobj locations,of
R4488 T14701 T14700 nummod four,locations
R4489 T14702 T14700 compound starting,locations
R4490 T14703 T14689 punct .,initiated
R4491 T14924 T14925 amod Rotating,rod
R4492 T14925 T14926 compound rod,test
R4493 T14928 T14929 compound Motor,coordination
R4494 T14929 T14930 nsubjpass coordination,assessed
R4495 T14931 T14930 auxpass was,assessed
R4496 T14932 T14930 prep with,assessed
R4497 T14933 T14934 det a,apparatus
R4498 T14934 T14932 pobj apparatus,with
R4499 T14935 T14936 amod rotating,rod
R4500 T14936 T14934 compound rod,apparatus
R4501 T14937 T14938 punct (,Seisakujo
R4502 T14938 T14934 parataxis Seisakujo,apparatus
R4503 T14939 T14938 dep KN,Seisakujo
R4504 T14940 T14939 punct -,KN
R4505 T14941 T14939 nummod 75,KN
R4506 T14942 T14938 punct ", ",Seisakujo
R4507 T14943 T14938 compound Natsume,Seisakujo
R4508 T14944 T14938 punct ", ",Seisakujo
R4509 T14945 T14938 npadvmod Tokyo,Seisakujo
R4510 T14946 T14938 punct ", ",Seisakujo
R4511 T14947 T14938 npadvmod Japan,Seisakujo
R4512 T14948 T14938 punct ),Seisakujo
R4513 T14949 T14934 punct ", ",apparatus
R4514 T14950 T14951 dep which,consisted
R4515 T14951 T14934 relcl consisted,apparatus
R4516 T14952 T14951 prep of,consisted
R4517 T14953 T14954 det a,rod
R4518 T14954 T14952 pobj rod,of
R4519 T14955 T14954 compound plastic,rod
R4520 T14956 T14957 punct (,diameter
R4521 T14957 T14954 parataxis diameter,rod
R4522 T14958 T14959 nummod 3,cm
R4523 T14959 T14957 compound cm,diameter
R4524 T14960 T14957 punct ", ",diameter
R4525 T14961 T14962 nummod 8,cm
R4526 T14962 T14963 npadvmod cm,long
R4527 T14963 T14957 amod long,diameter
R4528 T14964 T14957 punct ),diameter
R4529 T14965 T14954 prep with,rod
R4530 T14966 T14967 det a,surface
R4531 T14967 T14965 pobj surface,with
R4532 T14968 T14967 amod gritted,surface
R4533 T14969 T14967 acl flanked,surface
R4534 T14970 T14969 agent by,flanked
R4535 T14971 T14972 nummod two,discs
R4536 T14972 T14970 pobj discs,by
R4537 T14973 T14972 amod large,discs
R4538 T14974 T14975 punct (,diameter
R4539 T14975 T14972 parataxis diameter,discs
R4540 T14976 T14977 nummod 40,cm
R4541 T14977 T14975 compound cm,diameter
R4542 T14978 T14975 punct ),diameter
R4543 T14979 T14930 punct .,assessed
R4544 T14981 T14982 det The,mice
R4545 T14982 T14983 nsubjpass mice,placed
R4546 T14984 T14983 auxpass were,placed
R4547 T14985 T14983 advmod first,placed
R4548 T14986 T14983 prep on,placed
R4549 T14987 T14988 det the,rod
R4550 T14988 T14986 pobj rod,on
R4551 T14989 T14988 amod stationary,rod
R4552 T14990 T14983 prep for,placed
R4553 T14991 T14992 nummod 4,trials
R4554 T14992 T14990 pobj trials,for
R4555 T14993 T14992 amod successive,trials
R4556 T14994 T14992 punct ", ",trials
R4557 T14995 T14992 acl followed,trials
R4558 T14996 T14995 agent by,followed
R4559 T14997 T14998 nummod 4,trials
R4560 T14998 T14996 pobj trials,by
R4561 T14999 T14998 prep at,trials
R4562 T15000 T15001 det a,speed
R4563 T15001 T14999 pobj speed,at
R4564 T15002 T15001 compound rotation,speed
R4565 T15003 T15001 prep of,speed
R4566 T15004 T15005 nummod 5,rpm
R4567 T15005 T15003 pobj rpm,of
R4568 T15006 T14998 punct ", ",trials
R4569 T15007 T15008 nummod 8,trials
R4570 T15008 T14998 conj trials,trials
R4571 T15009 T15008 prep at,trials
R4572 T15010 T15011 nummod 10,rpm
R4573 T15011 T15009 pobj rpm,at
R4574 T15012 T15008 punct ", ",trials
R4575 T15013 T15008 cc and,trials
R4576 T15014 T15015 nummod 20,trials
R4577 T15015 T15008 conj trials,trials
R4578 T15016 T15015 prep at,trials
R4579 T15017 T15018 nummod 15,rpm
R4580 T15018 T15016 pobj rpm,at
R4581 T15019 T14998 prep in,trials
R4582 T15020 T15021 det that,order
R4583 T15021 T15019 pobj order,in
R4584 T15022 T14983 punct .,placed
R4585 T15024 T15025 nsubjpass Latency,monitored
R4586 T15026 T15027 mark until,occurred
R4587 T15027 T15024 advcl occurred,Latency
R4588 T15028 T15029 det a,fall
R4589 T15029 T15027 nsubj fall,occurred
R4590 T15030 T15025 auxpass was,monitored
R4591 T15031 T15025 prep for,monitored
R4592 T15032 T15033 nummod 120,sec
R4593 T15033 T15031 pobj sec,for
R4594 T15034 T15025 punct .,monitored
R4595 T15036 T15037 amod Intra-trial,intervals
R4596 T15037 T15038 nsubj intervals,were
R4597 T15039 T15037 prep for,intervals
R4598 T15040 T15041 det each,animal
R4599 T15041 T15039 pobj animal,for
R4600 T15042 T15043 amod more,20
R4601 T15043 T15045 nummod 20,min
R4602 T15044 T15043 quantmod than,20
R4603 T15045 T15038 attr min,were
R4604 T15046 T15038 punct .,were
R4605 T15164 T15165 compound Traction,test
R4606 T15167 T15168 det The,strength
R4607 T15168 T15170 nsubjpass strength,measured
R4608 T15169 T15168 compound grip,strength
R4609 T15171 T15168 prep of,strength
R4610 T15172 T15173 det each,mouse
R4611 T15173 T15171 pobj mouse,of
R4612 T15174 T15170 auxpass was,measured
R4613 T15175 T15170 prep with,measured
R4614 T15176 T15177 det a,apparatus
R4615 T15177 T15175 pobj apparatus,with
R4616 T15178 T15177 compound traction,apparatus
R4617 T15179 T15180 punct (,Co.
R4618 T15180 T15177 parataxis Co.,apparatus
R4619 T15181 T15180 dep FU,Co.
R4620 T15182 T15181 punct -,FU
R4621 T15183 T15181 nummod 1,FU
R4622 T15184 T15180 punct ", ",Co.
R4623 T15185 T15180 compound Muromachi,Co.
R4624 T15186 T15180 compound Kikai,Co.
R4625 T15187 T15180 punct ", ",Co.
R4626 T15188 T15180 amod Ltd.,Co.
R4627 T15189 T15180 punct ),Co.
R4628 T15190 T15170 punct .,measured
R4629 T15192 T15193 det Each,mouse
R4630 T15193 T15194 nsubjpass mouse,made
R4631 T15195 T15194 auxpass was,made
R4632 T15196 T15197 aux to,grasp
R4633 T15197 T15194 xcomp grasp,made
R4634 T15198 T15199 det the,bar
R4635 T15199 T15197 dobj bar,grasp
R4636 T15200 T15199 amod attached,bar
R4637 T15201 T15202 punct (,diameter
R4638 T15202 T15199 parataxis diameter,bar
R4639 T15203 T15204 nummod 2,mm
R4640 T15204 T15202 compound mm,diameter
R4641 T15205 T15202 punct ),diameter
R4642 T15206 T15197 prep with,grasp
R4643 T15207 T15208 det the,forepaws
R4644 T15208 T15206 pobj forepaws,with
R4645 T15209 T15194 cc and,made
R4646 T15210 T15211 auxpass was,pulled
R4647 T15211 T15194 conj pulled,made
R4648 T15212 T15211 advmod slowly,pulled
R4649 T15213 T15211 advmod back,pulled
R4650 T15214 T15211 prep by,pulled
R4651 T15215 T15216 det the,tail
R4652 T15216 T15214 pobj tail,by
R4653 T15217 T15194 punct .,made
R4654 T15219 T15220 det The,tension
R4655 T15220 T15222 nsubjpass tension,recorded
R4656 T15221 T15220 amod maximum,tension
R4657 T15223 T15220 prep before,tension
R4658 T15224 T15223 pobj release,before
R4659 T15225 T15222 auxpass was,recorded
R4660 T15226 T15222 punct .,recorded
R4661 T15486 T15487 compound Wire,suspension
R4662 T15487 T15488 compound suspension,test
R4663 T15490 T15491 det A,mouse
R4664 T15491 T15492 nsubjpass mouse,placed
R4665 T15493 T15492 auxpass was,placed
R4666 T15494 T15492 prep on,placed
R4667 T15495 T15496 det a,bar
R4668 T15496 T15494 pobj bar,on
R4669 T15497 T15496 amod stainless,bar
R4670 T15498 T15499 punct (,diameter
R4671 T15499 T15496 parataxis diameter,bar
R4672 T15500 T15501 nummod 50,cm
R4673 T15501 T15502 compound cm,length
R4674 T15502 T15499 dep length,diameter
R4675 T15503 T15499 punct ", ",diameter
R4676 T15504 T15505 nummod 2,mm
R4677 T15505 T15499 compound mm,diameter
R4678 T15506 T15499 punct ", ",diameter
R4679 T15507 T15499 advcl elevated,diameter
R4680 T15508 T15509 advmod up,37
R4681 T15509 T15511 nummod 37,cm
R4682 T15510 T15509 quantmod to,37
R4683 T15511 T15507 npadvmod cm,elevated
R4684 T15512 T15511 prep from,cm
R4685 T15513 T15514 det a,surface
R4686 T15514 T15512 pobj surface,from
R4687 T15515 T15499 punct ),diameter
R4688 T15516 T15492 prep at,placed
R4689 T15517 T15518 det a,midway
R4690 T15518 T15516 pobj midway,at
R4691 T15519 T15518 compound point,midway
R4692 T15520 T15518 prep between,midway
R4693 T15521 T15522 det the,supports
R4694 T15522 T15520 pobj supports,between
R4695 T15523 T15492 cc and,placed
R4696 T15524 T15492 conj observed,placed
R4697 T15525 T15524 prep for,observed
R4698 T15526 T15527 nummod 30,sec
R4699 T15527 T15525 pobj sec,for
R4700 T15528 T15524 prep in,observed
R4701 T15529 T15530 nummod four,trials
R4702 T15530 T15528 pobj trials,in
R4703 T15531 T15530 amod separate,trials
R4704 T15532 T15492 punct .,placed
R4705 T15534 T15535 det The,amount
R4706 T15535 T15536 nsubjpass amount,recorded
R4707 T15537 T15535 prep of,amount
R4708 T15538 T15537 pobj time,of
R4709 T15539 T15538 acl spent,time
R4710 T15540 T15539 advcl hanging,spent
R4711 T15541 T15536 auxpass was,recorded
R4712 T15542 T15536 cc and,recorded
R4713 T15543 T15536 conj scored,recorded
R4714 T15544 T15543 prep according,scored
R4715 T15545 T15544 prep to,according
R4716 T15546 T15547 det the,system
R4717 T15547 T15545 pobj system,to
R4718 T15548 T15547 amod following,system
R4719 T15549 T15550 punct [,41
R4720 T15550 T15543 parataxis 41,scored
R4721 T15551 T15550 punct ],41
R4722 T15552 T15536 punct : ,recorded
R4723 T15553 T15554 dep 0,fell
R4724 T15554 T15556 dep fell,escaped
R4725 T15555 T15554 punct ", ",fell
R4726 T15556 T15536 dep escaped,recorded
R4727 T15557 T15554 prt off,fell
R4728 T15558 T15556 punct ;,escaped
R4729 T15559 T15560 dep 1,hung
R4730 T15560 T15556 dep hung,escaped
R4731 T15561 T15560 punct ", ",hung
R4732 T15562 T15560 prep onto,hung
R4733 T15563 T15564 det the,bar
R4734 T15564 T15562 pobj bar,onto
R4735 T15565 T15560 prep with,hung
R4736 T15566 T15567 nummod two,forepaws
R4737 T15567 T15565 pobj forepaws,with
R4738 T15568 T15556 punct ;,escaped
R4739 T15569 T15570 dep 2,attempted
R4740 T15570 T15556 dep attempted,escaped
R4741 T15571 T15570 punct ", ",attempted
R4742 T15572 T15570 prep in,attempted
R4743 T15573 T15572 pobj addition,in
R4744 T15574 T15573 prep to,addition
R4745 T15575 T15574 pobj 1,to
R4746 T15576 T15570 punct ", ",attempted
R4747 T15577 T15578 aux to,climb
R4748 T15578 T15570 xcomp climb,attempted
R4749 T15579 T15578 prep onto,climb
R4750 T15580 T15581 det the,bar
R4751 T15581 T15579 pobj bar,onto
R4752 T15582 T15556 punct ;,escaped
R4753 T15583 T15584 dep 3,hung
R4754 T15584 T15556 dep hung,escaped
R4755 T15585 T15584 punct ", ",hung
R4756 T15586 T15584 prep onto,hung
R4757 T15587 T15588 det the,bar
R4758 T15588 T15586 pobj bar,onto
R4759 T15589 T15584 prep with,hung
R4760 T15590 T15591 nummod two,forepaws
R4761 T15591 T15589 pobj forepaws,with
R4762 T15592 T15591 cc and,forepaws
R4763 T15593 T15594 nummod one,paws
R4764 T15594 T15591 conj paws,forepaws
R4765 T15595 T15593 cc or,one
R4766 T15596 T15593 preconj both,one
R4767 T15597 T15594 compound hind,paws
R4768 T15598 T15556 punct ;,escaped
R4769 T15599 T15600 dep 4,hung
R4770 T15600 T15556 dep hung,escaped
R4771 T15601 T15600 punct ", ",hung
R4772 T15602 T15600 prep onto,hung
R4773 T15603 T15604 det the,bar
R4774 T15604 T15602 pobj bar,onto
R4775 T15605 T15600 prep with,hung
R4776 T15606 T15607 det all,paws
R4777 T15607 T15605 pobj paws,with
R4778 T15608 T15607 nummod four,paws
R4779 T15609 T15600 prep with,hung
R4780 T15610 T15609 pobj tail,with
R4781 T15611 T15610 acl wrapped,tail
R4782 T15612 T15611 prep around,wrapped
R4783 T15613 T15614 det the,bar
R4784 T15614 T15612 pobj bar,around
R4785 T15615 T15556 punct ;,escaped
R4786 T15616 T15556 dep 5,escaped
R4787 T15617 T15556 punct ", ",escaped
R4788 T15618 T15556 prep to,escaped
R4789 T15619 T15618 pobj one,to
R4790 T15620 T15619 prep of,one
R4791 T15621 T15622 det the,supports
R4792 T15622 T15620 pobj supports,of
R4793 T15623 T15536 punct .,recorded
R4794 T15705 T15706 compound Footprint,test
R4795 T15708 T15709 amod Black,ink
R4796 T15709 T15710 nsubjpass ink,applied
R4797 T15711 T15710 auxpass was,applied
R4798 T15712 T15710 prep to,applied
R4799 T15713 T15714 det the,paws
R4800 T15714 T15712 pobj paws,to
R4801 T15715 T15714 compound hind,paws
R4802 T15716 T15714 prep of,paws
R4803 T15717 T15718 det each,mouse
R4804 T15718 T15716 pobj mouse,of
R4805 T15719 T15710 cc and,applied
R4806 T15720 T15721 det the,mice
R4807 T15721 T15722 nsubjpass mice,placed
R4808 T15722 T15710 conj placed,applied
R4809 T15723 T15722 auxpass were,placed
R4810 T15724 T15722 prep in,placed
R4811 T15725 T15726 det a,alley
R4812 T15726 T15724 pobj alley,in
R4813 T15727 T15726 amod narrow,alley
R4814 T15728 T15729 punct (,cm
R4815 T15729 T15726 parataxis cm,alley
R4816 T15730 T15731 nummod 9,10
R4817 T15731 T15729 nummod 10,cm
R4818 T15732 T15731 punct ×,10
R4819 T15733 T15731 nummod 25,10
R4820 T15734 T15731 punct ×,10
R4821 T15735 T15729 punct ),cm
R4822 T15736 T15722 prep on,placed
R4823 T15737 T15738 amod white,paper
R4824 T15738 T15736 pobj paper,on
R4825 T15739 T15722 punct .,placed
R4826 T15741 T15742 compound Stride,length
R4827 T15742 T15743 nsubjpass length,calculated
R4828 T15744 T15742 cc and,length
R4829 T15745 T15746 compound step,width
R4830 T15746 T15742 conj width,length
R4831 T15747 T15743 auxpass were,calculated
R4832 T15748 T15743 punct .,calculated
R4833 T15951 T15952 nsubjpass Data,expressed
R4834 T15953 T15952 auxpass are,expressed
R4835 T15954 T15952 prep as,expressed
R4836 T15955 T15956 det the,SEM
R4837 T15956 T15954 pobj SEM,as
R4838 T15957 T15956 nmod means,SEM
R4839 T15958 T15956 punct ±,SEM
R4840 T15959 T15952 punct .,expressed
R4841 T15961 T15962 amod Statistical,analysis
R4842 T15962 T15963 nsubjpass analysis,conducted
R4843 T15964 T15963 auxpass was,conducted
R4844 T15965 T15963 advcl using,conducted
R4845 T15966 T15967 det the,package
R4846 T15967 T15965 dobj package,using
R4847 T15968 T15967 compound software,package
R4848 T15969 T15967 appos SAS,package
R4849 T15970 T15969 nummod 8.1,SAS
R4850 T15971 T15972 punct (,Japan
R4851 T15972 T15967 parataxis Japan,package
R4852 T15973 T15972 compound SAS,Japan
R4853 T15974 T15972 compound Institute,Japan
R4854 T15975 T15972 amod Ltd.,Japan
R4855 T15976 T15972 punct ", ",Japan
R4856 T15977 T15972 npadvmod Tokyo,Japan
R4857 T15978 T15972 punct ", ",Japan
R4858 T15979 T15972 npadvmod Japan,Japan
R4859 T15980 T15972 punct ),Japan
R4860 T15981 T15963 punct .,conducted
R4861 T15983 T15984 amod Statistical,significance
R4862 T15984 T15985 nsubjpass significance,determined
R4863 T15986 T15985 auxpass was,determined
R4864 T15987 T15985 prep by,determined
R4865 T15988 T15987 pobj analysis,by
R4866 T15989 T15988 prep of,analysis
R4867 T15990 T15989 pobj variance,of
R4868 T15991 T15988 punct (,analysis
R4869 T15992 T15988 appos ANOVA,analysis
R4870 T15993 T15988 punct ),analysis
R4871 T15994 T15988 acl followed,analysis
R4872 T15995 T15994 agent by,followed
R4873 T15996 T15997 det the,test
R4874 T15997 T15995 pobj test,by
R4875 T15998 T15999 nmod Dunnett,type
R4876 T15999 T15997 nmod type,test
R4877 T16000 T15999 punct -,type
R4878 T16001 T16002 amod multiple,comparison
R4879 T16002 T15997 compound comparison,test
R4880 T16003 T15985 punct ", ",determined
R4881 T16004 T15985 cc and,determined
R4882 T16005 T16006 compound p,values
R4883 T16006 T16007 nsubjpass values,considered
R4884 T16007 T15985 conj considered,determined
R4885 T16008 T16006 prep of,values
R4886 T16009 T16010 amod less,0.05
R4887 T16010 T16008 pobj 0.05,of
R4888 T16011 T16010 quantmod than,0.05
R4889 T16012 T16007 auxpass were,considered
R4890 T16013 T16014 aux to,be
R4891 T16014 T16007 xcomp be,considered
R4892 T16015 T16014 acomp significant,be
R4893 T16016 T16014 prep in,be
R4894 T16017 T16018 det the,task
R4895 T16018 T16016 pobj task,in
R4896 T16019 T16020 compound water,maze
R4897 T16020 T16018 compound maze,task
R4898 T16021 T16007 punct .,considered
R4899 T16023 T16024 amod Behavioural,data
R4900 T16024 T16025 nsubjpass data,analysed
R4901 T16026 T16024 prep in,data
R4902 T16027 T16028 det the,test
R4903 T16028 T16026 pobj test,in
R4904 T16029 T16030 amod rotating,rod
R4905 T16030 T16028 compound rod,test
R4906 T16031 T16025 auxpass were,analysed
R4907 T16032 T16025 agent by,analysed
R4908 T16033 T16034 nummod one,way
R4909 T16034 T16039 compound way,ANOVA
R4910 T16035 T16033 punct -,one
R4911 T16036 T16033 cc or,one
R4912 T16037 T16033 conj two,one
R4913 T16038 T16034 punct -,way
R4914 T16039 T16032 pobj ANOVA,by
R4915 T16040 T16039 prep with,ANOVA
R4916 T16041 T16042 amod repeated,measures
R4917 T16042 T16040 pobj measures,with
R4918 T16043 T16025 punct ", ",analysed
R4919 T16044 T16025 cc and,analysed
R4920 T16045 T16046 compound p,values
R4921 T16046 T16047 nsubjpass values,considered
R4922 T16047 T16025 conj considered,analysed
R4923 T16048 T16046 prep of,values
R4924 T16049 T16050 amod less,0.05
R4925 T16050 T16048 pobj 0.05,of
R4926 T16051 T16050 quantmod than,0.05
R4927 T16052 T16047 auxpass were,considered
R4928 T16053 T16054 aux to,be
R4929 T16054 T16047 xcomp be,considered
R4930 T16055 T16054 acomp significant,be
R4931 T16056 T16047 punct .,considered
R4932 T16223 T16224 nsubjpass Sections,fixed
R4933 T16225 T16223 prep of,Sections
R4934 T16226 T16225 pobj tissues,of
R4935 T16227 T16226 prep including,tissues
R4936 T16228 T16229 det the,brain
R4937 T16229 T16227 pobj brain,including
R4938 T16230 T16229 punct ", ",brain
R4939 T16231 T16232 amod spinal,cord
R4940 T16232 T16229 conj cord,brain
R4941 T16233 T16232 punct ", ",cord
R4942 T16234 T16232 conj heart,cord
R4943 T16235 T16234 punct ", ",heart
R4944 T16236 T16234 conj lung,heart
R4945 T16237 T16236 punct ", ",lung
R4946 T16238 T16236 conj liver,lung
R4947 T16239 T16238 punct ", ",liver
R4948 T16240 T16238 conj kidney,liver
R4949 T16241 T16240 punct ", ",kidney
R4950 T16242 T16240 conj spleen,kidney
R4951 T16243 T16242 cc and,spleen
R4952 T16244 T16242 conj testis,spleen
R4953 T16245 T16223 prep from,Sections
R4954 T16246 T16245 pobj mice,from
R4955 T16247 T16248 punct (,+
R4956 T16248 T16246 punct +,mice
R4957 T16249 T16248 punct +,+
R4958 T16250 T16248 punct /,+
R4959 T16251 T16248 punct ", ",+
R4960 T16252 T16253 punct +,-
R4961 T16253 T16248 conj -,+
R4962 T16254 T16253 punct /,-
R4963 T16255 T16253 cc and,-
R4964 T16256 T16257 punct -,-
R4965 T16257 T16253 conj -,-
R4966 T16258 T16257 punct /,-
R4967 T16259 T16248 punct ;,+
R4968 T16260 T16261 nsubj n,7
R4969 T16261 T16248 ccomp 7,+
R4970 T16262 T16261 punct =,7
R4971 T16263 T16261 punct ", ",7
R4972 T16264 T16261 conj 7,7
R4973 T16265 T16264 cc and,7
R4974 T16266 T16264 conj 7,7
R4975 T16267 T16261 punct ", ",7
R4976 T16268 T16261 advmod respectively,7
R4977 T16269 T16248 punct ),+
R4978 T16270 T16224 auxpass were,fixed
R4979 T16271 T16224 prep in,fixed
R4980 T16272 T16273 nummod 10,%
R4981 T16273 T16274 nmod %,formalin
R4982 T16274 T16271 pobj formalin,in
R4983 T16275 T16274 amod buffered,formalin
R4984 T16276 T16224 cc and,fixed
R4985 T16277 T16224 conj embedded,fixed
R4986 T16278 T16277 prep in,embedded
R4987 T16279 T16278 pobj paraffin,in
R4988 T16280 T16224 punct .,fixed
R4989 T16282 T16283 det Each,section
R4990 T16283 T16285 nsubjpass section,stained
R4991 T16284 T16283 compound paraffin,section
R4992 T16286 T16283 prep of,section
R4993 T16287 T16288 amod thick,μm
R4994 T16288 T16286 pobj μm,of
R4995 T16289 T16288 nummod 4,μm
R4996 T16290 T16285 auxpass was,stained
R4997 T16291 T16285 prep with,stained
R4998 T16292 T16291 pobj hematoxylin,with
R4999 T16293 T16292 cc and,hematoxylin
R5000 T16294 T16292 conj eosin,hematoxylin
R5001 T16295 T16292 punct (,hematoxylin
R5002 T16296 T16292 appos HE,hematoxylin
R5003 T16297 T16285 punct ),stained
R5004 T16298 T16285 punct .,stained