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PMC:1420271 / 9403-10267 JSONTXT 4 Projects

Annnotations TAB TSV DIC JSON TextAE

Id Subject Object Predicate Lexical cue
T13078 0-8 VBN denotes Targeted
T13079 9-19 NN denotes disruption
T13080 20-22 IN denotes of
T13081 23-26 DT denotes the
T13083 27-32 NN denotes mouse
T13084 33-37 NN denotes ESG1
T13082 38-42 NN denotes gene
T13085 42-43 . denotes .
T13086 43-66 sentence denotes A) Targeting strategy.
T13087 44-45 LS denotes A
T13089 45-46 -RRB- denotes )
T13090 47-56 NN denotes Targeting
T13088 57-65 NN denotes strategy
T13091 65-66 . denotes .
T13092 66-115 sentence denotes Homologous regions are indicated by thick lines.
T13093 67-77 JJ denotes Homologous
T13094 78-85 NNS denotes regions
T13096 86-89 VBP denotes are
T13095 90-99 VBN denotes indicated
T13097 100-102 IN denotes by
T13098 103-108 JJ denotes thick
T13099 109-114 NNS denotes lines
T13100 114-115 . denotes .
T13101 115-214 sentence denotes Recognition sites of PstI (P) and SpeI (S), which were used for Southern blot analyses, are shown.
T13102 116-127 NN denotes Recognition
T13103 128-133 NNS denotes sites
T13105 134-136 IN denotes of
T13106 137-141 NN denotes PstI
T13107 142-143 -LRB- denotes (
T13108 143-144 NN denotes P
T13109 144-145 -RRB- denotes )
T13110 146-149 CC denotes and
T13111 150-154 NN denotes SpeI
T13112 155-156 -LRB- denotes (
T13113 156-157 NN denotes S
T13114 157-158 -RRB- denotes )
T13115 158-160 , denotes ,
T13116 160-165 WDT denotes which
T13118 166-170 VBD denotes were
T13117 171-175 VBN denotes used
T13119 176-179 IN denotes for
T13120 180-188 NNP denotes Southern
T13121 189-193 NN denotes blot
T13122 194-202 NNS denotes analyses
T13123 202-204 , denotes ,
T13124 204-207 VBP denotes are
T13104 208-213 VBN denotes shown
T13125 213-214 . denotes .
T13126 214-343 sentence denotes The gene encoding diphtheria toxin A (DTA) was inserted at the 3' end of the targeting vectors to facilitate negative selection.
T13127 215-218 DT denotes The
T13129 219-223 NN denotes gene
T13130 224-232 VBG denotes encoding
T13131 233-243 NN denotes diphtheria
T13132 244-249 NN denotes toxin
T13128 250-251 NN denotes A
T13134 252-253 -LRB- denotes (
T13135 253-256 NN denotes DTA
T13136 256-257 -RRB- denotes )
T13137 258-261 VBD denotes was
T13133 262-270 VBN denotes inserted
T13138 271-273 IN denotes at
T13139 274-277 DT denotes the
T13141 278-279 CD denotes 3
T13142 279-280 SYM denotes '
T13140 281-284 NN denotes end
T13143 285-287 IN denotes of
T13144 288-291 DT denotes the
T13146 292-301 NN denotes targeting
T13145 302-309 NNS denotes vectors
T13147 310-312 TO denotes to
T13148 313-323 VB denotes facilitate
T13149 324-332 JJ denotes negative
T13150 333-342 NN denotes selection
T13151 342-343 . denotes .
T13152 343-406 sentence denotes B) Southern blot analyses confirming homologous recombination.
T13153 344-345 LS denotes B
T13155 345-346 -RRB- denotes )
T13156 347-355 NNP denotes Southern
T13157 356-360 NN denotes blot
T13154 361-369 NNS denotes analyses
T13158 370-380 VBG denotes confirming
T13159 381-391 JJ denotes homologous
T13160 392-405 NN denotes recombination
T13161 405-406 . denotes .
T13162 406-500 sentence denotes WT, wild-type ES cells; β, β-geo +/- ES cells; H, HygR +/- ES cells; -/-, ESG1-null ES cells.
T13163 407-409 NN denotes WT
T13164 409-411 , denotes ,
T13165 411-415 JJ denotes wild
T13167 415-416 HYPH denotes -
T13166 416-420 NN denotes type
T13169 421-423 NN denotes ES
T13168 424-429 NNS denotes cells
T13170 429-430 : denotes ;
T13171 431-432 NN denotes β
T13172 432-434 , denotes ,
T13173 434-435 NN denotes β
T13175 435-436 HYPH denotes -
T13174 436-439 NN denotes geo
T13177 440-441 SYM denotes +
T13178 441-442 HYPH denotes /
T13179 442-443 SYM denotes -
T13180 444-446 NN denotes ES
T13176 447-452 NNS denotes cells
T13181 452-453 : denotes ;
T13182 454-455 NN denotes H
T13183 455-457 , denotes ,
T13184 457-461 NN denotes HygR
T13186 462-463 SYM denotes +
T13187 463-464 HYPH denotes /
T13188 464-465 SYM denotes -
T13189 466-468 NN denotes ES
T13185 469-474 NNS denotes cells
T13190 474-475 : denotes ;
T13191 476-477 SYM denotes -
T13193 477-478 HYPH denotes /
T13192 478-479 SYM denotes -
T13194 479-481 , denotes ,
T13195 481-485 NN denotes ESG1
T13197 485-486 HYPH denotes -
T13196 486-490 JJ denotes null
T13199 491-493 NN denotes ES
T13198 494-499 NNS denotes cells
T13200 499-500 . denotes .
T13201 500-532 sentence denotes Numbers indicate clone numbers.
T13202 501-508 NNS denotes Numbers
T13203 509-517 VBP denotes indicate
T13204 518-523 NN denotes clone
T13205 524-531 NNS denotes numbers
T13206 531-532 . denotes .
T13207 532-691 sentence denotes C) Northern blot (upper) and western blot (lower) analyses of wild-type ES cells (WT), ESG1-null ES cells (-/-, three clones) and heterozygous ES cells (+/-).
T13208 533-534 LS denotes C
T13210 534-535 -RRB- denotes )
T13211 536-544 NNP denotes Northern
T13212 545-549 NN denotes blot
T13213 550-551 -LRB- denotes (
T13214 551-556 JJ denotes upper
T13215 556-557 -RRB- denotes )
T13216 558-561 CC denotes and
T13217 562-569 NNP denotes western
T13218 570-574 NN denotes blot
T13219 575-576 -LRB- denotes (
T13220 576-581 JJ denotes lower
T13221 581-582 -RRB- denotes )
T13209 583-591 NNS denotes analyses
T13222 592-594 IN denotes of
T13223 595-599 JJ denotes wild
T13225 599-600 HYPH denotes -
T13224 600-604 NN denotes type
T13227 605-607 NN denotes ES
T13226 608-613 NNS denotes cells
T13228 614-615 -LRB- denotes (
T13229 615-617 NN denotes WT
T13230 617-618 -RRB- denotes )
T13231 618-620 , denotes ,
T13232 620-624 NN denotes ESG1
T13234 624-625 HYPH denotes -
T13233 625-629 JJ denotes null
T13236 630-632 NN denotes ES
T13235 633-638 NNS denotes cells
T13237 639-640 -LRB- denotes (
T13239 640-641 SYM denotes -
T13241 641-642 HYPH denotes /
T13240 642-643 SYM denotes -
T13242 643-645 , denotes ,
T13243 645-650 CD denotes three
T13238 651-657 NNS denotes clones
T13244 657-658 -RRB- denotes )
T13245 659-662 CC denotes and
T13246 663-675 JJ denotes heterozygous
T13248 676-678 NN denotes ES
T13247 679-684 NNS denotes cells
T13249 685-686 -LRB- denotes (
T13251 686-687 SYM denotes +
T13252 687-688 HYPH denotes /
T13250 688-689 SYM denotes -
T13253 689-690 -RRB- denotes )
T13254 690-691 . denotes .
T13255 691-749 sentence denotes Northern blot was performed as previously described [20].
T13256 692-700 NNP denotes Northern
T13257 701-705 NN denotes blot
T13259 706-709 VBD denotes was
T13258 710-719 VBN denotes performed
T13260 720-722 IN denotes as
T13262 723-733 RB denotes previously
T13261 734-743 VBN denotes described
T13263 744-745 -LRB- denotes [
T13264 745-747 CD denotes 20
T13265 747-748 -RRB- denotes ]
T13266 748-749 . denotes .
T13267 749-864 sentence denotes To confirm the loading of equal amounts of RNA, ethidium bromide staining of ribosomal RNA is also shown (middle).
T13268 750-752 TO denotes To
T13269 753-760 VB denotes confirm
T13271 761-764 DT denotes the
T13272 765-772 NN denotes loading
T13273 773-775 IN denotes of
T13274 776-781 JJ denotes equal
T13275 782-789 NNS denotes amounts
T13276 790-792 IN denotes of
T13277 793-796 NN denotes RNA
T13278 796-798 , denotes ,
T13279 798-806 NN denotes ethidium
T13280 807-814 NN denotes bromide
T13281 815-823 NN denotes staining
T13282 824-826 IN denotes of
T13283 827-836 JJ denotes ribosomal
T13284 837-840 NN denotes RNA
T13285 841-843 VBZ denotes is
T13286 844-848 RB denotes also
T13270 849-854 VBN denotes shown
T13287 855-856 -LRB- denotes (
T13288 856-862 JJ denotes middle
T13289 862-863 -RRB- denotes )
T13290 863-864 . denotes .
R3642 T13078 T13079 amod Targeted,disruption
R3643 T13080 T13079 prep of,disruption
R3644 T13081 T13082 det the,gene
R3645 T13082 T13080 pobj gene,of
R3646 T13083 T13082 compound mouse,gene
R3647 T13084 T13082 compound ESG1,gene
R3648 T13085 T13079 punct .,disruption
R3649 T13087 T13088 meta A,strategy
R3650 T13089 T13087 punct ),A
R3651 T13090 T13088 compound Targeting,strategy
R3652 T13091 T13088 punct .,strategy
R3653 T13093 T13094 amod Homologous,regions
R3654 T13094 T13095 nsubjpass regions,indicated
R3655 T13096 T13095 auxpass are,indicated
R3656 T13097 T13095 agent by,indicated
R3657 T13098 T13099 amod thick,lines
R3658 T13099 T13097 pobj lines,by
R3659 T13100 T13095 punct .,indicated
R3660 T13102 T13103 compound Recognition,sites
R3661 T13103 T13104 nsubjpass sites,shown
R3662 T13105 T13103 prep of,sites
R3663 T13106 T13105 pobj PstI,of
R3664 T13107 T13108 punct (,P
R3665 T13108 T13106 parataxis P,PstI
R3666 T13109 T13108 punct ),P
R3667 T13110 T13106 cc and,PstI
R3668 T13111 T13106 conj SpeI,PstI
R3669 T13112 T13113 punct (,S
R3670 T13113 T13111 parataxis S,SpeI
R3671 T13114 T13113 punct ),S
R3672 T13115 T13103 punct ", ",sites
R3673 T13116 T13117 dep which,used
R3674 T13117 T13103 relcl used,sites
R3675 T13118 T13117 auxpass were,used
R3676 T13119 T13117 prep for,used
R3677 T13120 T13121 compound Southern,blot
R3678 T13121 T13122 compound blot,analyses
R3679 T13122 T13119 pobj analyses,for
R3680 T13123 T13104 punct ", ",shown
R3681 T13124 T13104 auxpass are,shown
R3682 T13125 T13104 punct .,shown
R3683 T13127 T13128 det The,A
R3684 T13128 T13133 nsubjpass A,inserted
R3685 T13129 T13128 nmod gene,A
R3686 T13130 T13128 amod encoding,A
R3687 T13131 T13128 compound diphtheria,A
R3688 T13132 T13128 compound toxin,A
R3689 T13134 T13128 punct (,A
R3690 T13135 T13128 appos DTA,A
R3691 T13136 T13133 punct ),inserted
R3692 T13137 T13133 auxpass was,inserted
R3693 T13138 T13133 prep at,inserted
R3694 T13139 T13140 det the,end
R3695 T13140 T13138 pobj end,at
R3696 T13141 T13140 nummod 3,end
R3697 T13142 T13141 punct ',3
R3698 T13143 T13140 prep of,end
R3699 T13144 T13145 det the,vectors
R3700 T13145 T13143 pobj vectors,of
R3701 T13146 T13145 compound targeting,vectors
R3702 T13147 T13148 aux to,facilitate
R3703 T13148 T13133 advcl facilitate,inserted
R3704 T13149 T13150 amod negative,selection
R3705 T13150 T13148 dobj selection,facilitate
R3706 T13151 T13133 punct .,inserted
R3707 T13153 T13154 meta B,analyses
R3708 T13155 T13153 punct ),B
R3709 T13156 T13157 compound Southern,blot
R3710 T13157 T13154 compound blot,analyses
R3711 T13158 T13154 acl confirming,analyses
R3712 T13159 T13160 amod homologous,recombination
R3713 T13160 T13158 dobj recombination,confirming
R3714 T13161 T13154 punct .,analyses
R3715 T13164 T13163 punct ", ",WT
R3716 T13165 T13166 amod wild,type
R3717 T13166 T13168 compound type,cells
R3718 T13167 T13166 punct -,type
R3719 T13168 T13163 appos cells,WT
R3720 T13169 T13168 compound ES,cells
R3721 T13170 T13163 punct ;,WT
R3722 T13171 T13163 appos β,WT
R3723 T13172 T13171 punct ", ",β
R3724 T13173 T13174 nmod β,geo
R3725 T13174 T13176 nmod geo,cells
R3726 T13175 T13174 punct -,geo
R3727 T13176 T13171 appos cells,β
R3728 T13177 T13174 punct +,geo
R3729 T13178 T13174 punct /,geo
R3730 T13179 T13174 punct -,geo
R3731 T13180 T13176 compound ES,cells
R3732 T13181 T13163 punct ;,WT
R3733 T13182 T13163 appos H,WT
R3734 T13183 T13182 punct ", ",H
R3735 T13184 T13185 nmod HygR,cells
R3736 T13185 T13182 appos cells,H
R3737 T13186 T13184 punct +,HygR
R3738 T13187 T13184 punct /,HygR
R3739 T13188 T13184 punct -,HygR
R3740 T13189 T13185 compound ES,cells
R3741 T13190 T13163 punct ;,WT
R3742 T13191 T13192 punct -,-
R3743 T13192 T13163 appos -,WT
R3744 T13193 T13192 punct /,-
R3745 T13194 T13192 punct ", ",-
R3746 T13195 T13196 npadvmod ESG1,null
R3747 T13196 T13198 amod null,cells
R3748 T13197 T13196 punct -,null
R3749 T13198 T13192 appos cells,-
R3750 T13199 T13198 compound ES,cells
R3751 T13200 T13163 punct .,WT
R3752 T13202 T13203 nsubj Numbers,indicate
R3753 T13204 T13205 compound clone,numbers
R3754 T13205 T13203 dobj numbers,indicate
R3755 T13206 T13203 punct .,indicate
R3756 T13208 T13209 meta C,analyses
R3757 T13210 T13208 punct ),C
R3758 T13211 T13212 nmod Northern,blot
R3759 T13212 T13209 nmod blot,analyses
R3760 T13213 T13214 punct (,upper
R3761 T13214 T13212 parataxis upper,blot
R3762 T13215 T13214 punct ),upper
R3763 T13216 T13212 cc and,blot
R3764 T13217 T13218 compound western,blot
R3765 T13218 T13212 conj blot,blot
R3766 T13219 T13220 punct (,lower
R3767 T13220 T13218 parataxis lower,blot
R3768 T13221 T13220 punct ),lower
R3769 T13222 T13209 prep of,analyses
R3770 T13223 T13224 amod wild,type
R3771 T13224 T13226 compound type,cells
R3772 T13225 T13224 punct -,type
R3773 T13226 T13222 pobj cells,of
R3774 T13227 T13226 compound ES,cells
R3775 T13228 T13229 punct (,WT
R3776 T13229 T13226 parataxis WT,cells
R3777 T13230 T13229 punct ),WT
R3778 T13231 T13226 punct ", ",cells
R3779 T13232 T13233 npadvmod ESG1,null
R3780 T13233 T13235 amod null,cells
R3781 T13234 T13233 punct -,null
R3782 T13235 T13226 conj cells,cells
R3783 T13236 T13235 compound ES,cells
R3784 T13237 T13238 punct (,clones
R3785 T13238 T13235 parataxis clones,cells
R3786 T13239 T13240 punct -,-
R3787 T13240 T13238 punct -,clones
R3788 T13241 T13240 punct /,-
R3789 T13242 T13238 punct ", ",clones
R3790 T13243 T13238 nummod three,clones
R3791 T13244 T13238 punct ),clones
R3792 T13245 T13235 cc and,cells
R3793 T13246 T13247 amod heterozygous,cells
R3794 T13247 T13235 conj cells,cells
R3795 T13248 T13247 compound ES,cells
R3796 T13249 T13250 punct (,-
R3797 T13250 T13247 punct -,cells
R3798 T13251 T13250 punct +,-
R3799 T13252 T13250 punct /,-
R3800 T13253 T13250 punct ),-
R3801 T13254 T13209 punct .,analyses
R3802 T13256 T13257 compound Northern,blot
R3803 T13257 T13258 nsubjpass blot,performed
R3804 T13259 T13258 auxpass was,performed
R3805 T13260 T13261 mark as,described
R3806 T13261 T13258 advcl described,performed
R3807 T13262 T13261 advmod previously,described
R3808 T13263 T13264 punct [,20
R3809 T13264 T13261 parataxis 20,described
R3810 T13265 T13264 punct ],20
R3811 T13266 T13258 punct .,performed
R3812 T13268 T13269 aux To,confirm
R3813 T13269 T13270 advcl confirm,shown
R3814 T13271 T13272 det the,loading
R3815 T13272 T13269 dobj loading,confirm
R3816 T13273 T13272 prep of,loading
R3817 T13274 T13275 amod equal,amounts
R3818 T13275 T13273 pobj amounts,of
R3819 T13276 T13275 prep of,amounts
R3820 T13277 T13276 pobj RNA,of
R3821 T13278 T13270 punct ", ",shown
R3822 T13279 T13280 compound ethidium,bromide
R3823 T13280 T13281 compound bromide,staining
R3824 T13281 T13270 nsubjpass staining,shown
R3825 T13282 T13281 prep of,staining
R3826 T13283 T13284 amod ribosomal,RNA
R3827 T13284 T13282 pobj RNA,of
R3828 T13285 T13270 auxpass is,shown
R3829 T13286 T13270 advmod also,shown
R3830 T13287 T13288 punct (,middle
R3831 T13288 T13270 parataxis middle,shown
R3832 T13289 T13288 punct ),middle
R3833 T13290 T13270 punct .,shown