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PMC:1420271 / 486-1601 JSONTXT 4 Projects

Annnotations TAB TSV DIC JSON TextAE

Id Subject Object Predicate Lexical cue
T894 0-1 DT denotes A
T896 2-7 NNP denotes Blast
T895 8-14 NN denotes search
T898 15-17 IN denotes of
T899 18-23 NN denotes mouse
T901 24-31 JJ denotes genomic
T900 32-41 NNS denotes databases
T897 42-48 VBD denotes failed
T902 49-51 TO denotes to
T903 52-58 VB denotes locate
T904 59-62 DT denotes the
T906 63-67 NN denotes ESG1
T905 68-72 NN denotes gene
T907 72-73 . denotes .
T908 73-240 sentence denotes We identified several bacterial artificial clones containing the mouse ESG1 gene and an additional ESG1-like sequence with a similar gene structure from chromosome 9.
T909 74-76 PRP denotes We
T910 77-87 VBD denotes identified
T911 88-95 JJ denotes several
T913 96-105 JJ denotes bacterial
T914 106-116 JJ denotes artificial
T912 117-123 NNS denotes clones
T915 124-134 VBG denotes containing
T916 135-138 DT denotes the
T918 139-144 NN denotes mouse
T919 145-149 NN denotes ESG1
T917 150-154 NN denotes gene
T920 155-158 CC denotes and
T921 159-161 DT denotes an
T923 162-172 JJ denotes additional
T924 173-177 NN denotes ESG1
T926 177-178 HYPH denotes -
T925 178-182 JJ denotes like
T922 183-191 NN denotes sequence
T927 192-196 IN denotes with
T928 197-198 DT denotes a
T930 199-206 JJ denotes similar
T931 207-211 NN denotes gene
T929 212-221 NN denotes structure
T932 222-226 IN denotes from
T933 227-237 NN denotes chromosome
T934 238-239 CD denotes 9
T935 239-240 . denotes .
T936 240-352 sentence denotes The ESG1-like sequence contained a multiple critical mutations, indicating that it was a duplicated pseudogene.
T937 241-244 DT denotes The
T939 245-249 NN denotes ESG1
T941 249-250 HYPH denotes -
T940 250-254 JJ denotes like
T938 255-263 NN denotes sequence
T942 264-273 VBD denotes contained
T943 274-275 DT denotes a
T945 276-284 JJ denotes multiple
T946 285-293 JJ denotes critical
T944 294-303 NNS denotes mutations
T947 303-305 , denotes ,
T948 305-315 VBG denotes indicating
T949 316-320 IN denotes that
T951 321-323 PRP denotes it
T950 324-327 VBD denotes was
T952 328-329 DT denotes a
T954 330-340 VBN denotes duplicated
T953 341-351 NN denotes pseudogene
T955 351-352 . denotes .
T956 352-532 sentence denotes The 5' flanking region of the ESG1 gene, but not that of the pseudogene, exhibited strong enhancer and promoter activity in undifferentiated ES cells by luciferase reporter assay.
T957 353-356 DT denotes The
T959 357-358 CD denotes 5
T960 358-359 SYM denotes '
T961 360-368 NN denotes flanking
T958 369-375 NN denotes region
T963 376-378 IN denotes of
T964 379-382 DT denotes the
T966 383-387 NN denotes ESG1
T965 388-392 NN denotes gene
T967 392-394 , denotes ,
T968 394-397 CC denotes but
T969 398-401 RB denotes not
T970 402-406 DT denotes that
T971 407-409 IN denotes of
T972 410-413 DT denotes the
T973 414-424 NN denotes pseudogene
T974 424-426 , denotes ,
T962 426-435 VBD denotes exhibited
T975 436-442 JJ denotes strong
T977 443-451 NN denotes enhancer
T978 452-455 CC denotes and
T979 456-464 NN denotes promoter
T976 465-473 NN denotes activity
T980 474-476 IN denotes in
T981 477-493 JJ denotes undifferentiated
T983 494-496 NN denotes ES
T982 497-502 NNS denotes cells
T984 503-505 IN denotes by
T985 506-516 NN denotes luciferase
T986 517-525 NN denotes reporter
T987 526-531 NN denotes assay
T988 531-532 . denotes .
T989 532-676 sentence denotes To study the physiological functions of the ESG1 gene, we replaced this sequence in ES cells with a β-geo cassette by homologous recombination.
T990 533-535 TO denotes To
T991 536-541 VB denotes study
T993 542-545 DT denotes the
T995 546-559 JJ denotes physiological
T994 560-569 NNS denotes functions
T996 570-572 IN denotes of
T997 573-576 DT denotes the
T999 577-581 NN denotes ESG1
T998 582-586 NN denotes gene
T1000 586-588 , denotes ,
T1001 588-590 PRP denotes we
T992 591-599 VBD denotes replaced
T1002 600-604 DT denotes this
T1003 605-613 NN denotes sequence
T1004 614-616 IN denotes in
T1005 617-619 NN denotes ES
T1006 620-625 NNS denotes cells
T1007 626-630 IN denotes with
T1008 631-632 DT denotes a
T1010 633-634 NN denotes β
T1012 634-635 HYPH denotes -
T1011 635-638 NN denotes geo
T1009 639-647 NN denotes cassette
T1013 648-650 IN denotes by
T1014 651-661 JJ denotes homologous
T1015 662-675 NN denotes recombination
T1016 675-676 . denotes .
T1017 676-787 sentence denotes Despite specific expression in early embryos and germ cells, ESG1-/- mice developed normally and were fertile.
T1018 677-684 IN denotes Despite
T1020 685-693 JJ denotes specific
T1021 694-704 NN denotes expression
T1022 705-707 IN denotes in
T1023 708-713 JJ denotes early
T1024 714-721 NNS denotes embryos
T1025 722-725 CC denotes and
T1026 726-730 NN denotes germ
T1027 731-736 NNS denotes cells
T1028 736-738 , denotes ,
T1029 738-742 NN denotes ESG1
T1031 742-743 SYM denotes -
T1032 743-744 HYPH denotes /
T1033 744-745 SYM denotes -
T1030 746-750 NNS denotes mice
T1019 751-760 VBD denotes developed
T1034 761-769 RB denotes normally
T1035 770-773 CC denotes and
T1036 774-778 VBD denotes were
T1037 779-786 JJ denotes fertile
T1038 786-787 . denotes .
T1039 787-946 sentence denotes We also generated ESG1-/- ES cells both by a second independent homologous recombination and directly from blastocysts derived from heterozygous intercrosses.
T1040 788-790 PRP denotes We
T1042 791-795 RB denotes also
T1041 796-805 VBD denotes generated
T1043 806-810 NN denotes ESG1
T1045 810-811 SYM denotes -
T1046 811-812 HYPH denotes /
T1047 812-813 SYM denotes -
T1048 814-816 NN denotes ES
T1044 817-822 NNS denotes cells
T1049 823-827 CC denotes both
T1050 828-830 IN denotes by
T1051 831-832 DT denotes a
T1053 833-839 JJ denotes second
T1054 840-851 JJ denotes independent
T1055 852-862 JJ denotes homologous
T1052 863-876 NN denotes recombination
T1056 877-880 CC denotes and
T1057 881-889 RB denotes directly
T1058 890-894 IN denotes from
T1059 895-906 NNS denotes blastocysts
T1060 907-914 VBN denotes derived
T1061 915-919 IN denotes from
T1062 920-932 JJ denotes heterozygous
T1063 933-945 NNS denotes intercrosses
T1064 945-946 . denotes .
T1065 946-1032 sentence denotes Northern blot and western blot analyses confirmed the absence of ESG1 in these cells.
T1066 947-955 NNP denotes Northern
T1067 956-960 NN denotes blot
T1069 961-964 CC denotes and
T1070 965-972 NNP denotes western
T1071 973-977 NN denotes blot
T1068 978-986 NNS denotes analyses
T1072 987-996 VBD denotes confirmed
T1073 997-1000 DT denotes the
T1074 1001-1008 NN denotes absence
T1075 1009-1011 IN denotes of
T1076 1012-1016 NN denotes ESG1
T1077 1017-1019 IN denotes in
T1078 1020-1025 DT denotes these
T1079 1026-1031 NNS denotes cells
T1080 1031-1032 . denotes .
T1081 1032-1115 sentence denotes These ES cells demonstrated normal morphology, proliferation, and differentiation.
T1082 1033-1038 DT denotes These
T1084 1039-1041 NN denotes ES
T1083 1042-1047 NNS denotes cells
T1085 1048-1060 VBD denotes demonstrated
T1086 1061-1067 JJ denotes normal
T1087 1068-1078 NN denotes morphology
T1088 1078-1080 , denotes ,
T1089 1080-1093 NN denotes proliferation
T1090 1093-1095 , denotes ,
T1091 1095-1098 CC denotes and
T1092 1099-1114 NN denotes differentiation
T1093 1114-1115 . denotes .
R100 T906 T905 compound ESG1,gene
R101 T907 T897 punct .,failed
R102 T909 T910 nsubj We,identified
R103 T911 T912 amod several,clones
R104 T912 T910 dobj clones,identified
R105 T913 T912 amod bacterial,clones
R106 T914 T912 amod artificial,clones
R107 T915 T912 acl containing,clones
R108 T916 T917 det the,gene
R109 T917 T915 dobj gene,containing
R110 T918 T917 compound mouse,gene
R111 T919 T917 compound ESG1,gene
R112 T920 T912 cc and,clones
R113 T921 T922 det an,sequence
R114 T922 T912 conj sequence,clones
R115 T923 T922 amod additional,sequence
R116 T924 T925 npadvmod ESG1,like
R117 T925 T922 amod like,sequence
R118 T926 T925 punct -,like
R119 T927 T922 prep with,sequence
R120 T928 T929 det a,structure
R121 T929 T927 pobj structure,with
R122 T930 T929 amod similar,structure
R123 T931 T929 compound gene,structure
R124 T932 T929 prep from,structure
R125 T933 T932 pobj chromosome,from
R126 T934 T933 nummod 9,chromosome
R127 T935 T910 punct .,identified
R128 T937 T938 det The,sequence
R129 T938 T942 nsubj sequence,contained
R130 T939 T940 npadvmod ESG1,like
R131 T940 T938 amod like,sequence
R132 T941 T940 punct -,like
R133 T943 T944 det a,mutations
R134 T944 T942 dobj mutations,contained
R135 T945 T944 amod multiple,mutations
R136 T946 T944 amod critical,mutations
R137 T947 T942 punct ", ",contained
R138 T948 T942 advcl indicating,contained
R139 T949 T950 mark that,was
R140 T950 T948 ccomp was,indicating
R141 T951 T950 nsubj it,was
R142 T952 T953 det a,pseudogene
R143 T953 T950 attr pseudogene,was
R144 T954 T953 amod duplicated,pseudogene
R145 T955 T942 punct .,contained
R146 T957 T958 det The,region
R147 T958 T962 nsubj region,exhibited
R148 T959 T958 nummod 5,region
R149 T960 T959 punct ',5
R150 T961 T958 compound flanking,region
R151 T963 T958 prep of,region
R152 T964 T965 det the,gene
R153 T965 T963 pobj gene,of
R154 T966 T965 compound ESG1,gene
R155 T967 T965 punct ", ",gene
R156 T968 T965 cc but,gene
R157 T969 T968 neg not,but
R158 T970 T965 conj that,gene
R159 T971 T970 prep of,that
R160 T972 T973 det the,pseudogene
R161 T973 T971 pobj pseudogene,of
R162 T974 T962 punct ", ",exhibited
R163 T975 T976 amod strong,activity
R164 T976 T962 dobj activity,exhibited
R165 T977 T976 nmod enhancer,activity
R166 T978 T977 cc and,enhancer
R167 T979 T977 conj promoter,enhancer
R168 T980 T962 prep in,exhibited
R169 T981 T982 amod undifferentiated,cells
R170 T982 T980 pobj cells,in
R171 T983 T982 compound ES,cells
R172 T984 T962 prep by,exhibited
R173 T985 T986 compound luciferase,reporter
R174 T986 T987 compound reporter,assay
R175 T987 T984 pobj assay,by
R176 T988 T962 punct .,exhibited
R177 T990 T991 aux To,study
R178 T991 T992 advcl study,replaced
R179 T993 T994 det the,functions
R180 T994 T991 dobj functions,study
R181 T995 T994 amod physiological,functions
R182 T996 T994 prep of,functions
R183 T997 T998 det the,gene
R184 T998 T996 pobj gene,of
R185 T999 T998 compound ESG1,gene
R186 T1000 T992 punct ", ",replaced
R187 T1001 T992 nsubj we,replaced
R188 T1002 T1003 det this,sequence
R189 T1003 T992 dobj sequence,replaced
R190 T1004 T1003 prep in,sequence
R191 T1005 T1006 compound ES,cells
R192 T1006 T1004 pobj cells,in
R193 T1007 T992 prep with,replaced
R194 T1008 T1009 det a,cassette
R195 T1009 T1007 pobj cassette,with
R196 T1010 T1011 compound β,geo
R197 T1011 T1009 compound geo,cassette
R198 T1012 T1011 punct -,geo
R199 T1013 T992 prep by,replaced
R200 T1014 T1015 amod homologous,recombination
R201 T1015 T1013 pobj recombination,by
R202 T1016 T992 punct .,replaced
R203 T1018 T1019 prep Despite,developed
R204 T1020 T1021 amod specific,expression
R205 T1021 T1018 pobj expression,Despite
R206 T1022 T1021 prep in,expression
R207 T1023 T1024 amod early,embryos
R208 T1024 T1022 pobj embryos,in
R209 T1025 T1024 cc and,embryos
R210 T1026 T1027 compound germ,cells
R211 T1027 T1024 conj cells,embryos
R212 T1028 T1019 punct ", ",developed
R213 T1029 T1030 nmod ESG1,mice
R214 T1030 T1019 nsubj mice,developed
R215 T1031 T1029 punct -,ESG1
R216 T1032 T1029 punct /,ESG1
R217 T1033 T1029 punct -,ESG1
R218 T1034 T1019 advmod normally,developed
R219 T1035 T1019 cc and,developed
R220 T1036 T1019 conj were,developed
R221 T1037 T1036 acomp fertile,were
R222 T1038 T1019 punct .,developed
R223 T1040 T1041 nsubj We,generated
R224 T1042 T1041 advmod also,generated
R225 T1043 T1044 nmod ESG1,cells
R226 T1044 T1041 dobj cells,generated
R227 T1045 T1043 punct -,ESG1
R228 T1046 T1043 punct /,ESG1
R229 T1047 T1043 punct -,ESG1
R230 T1048 T1044 compound ES,cells
R231 T1049 T1050 preconj both,by
R232 T1050 T1041 prep by,generated
R233 T1051 T1052 det a,recombination
R234 T1052 T1050 pobj recombination,by
R235 T1053 T1052 amod second,recombination
R236 T1054 T1052 amod independent,recombination
R237 T1055 T1052 amod homologous,recombination
R238 T1056 T1050 cc and,by
R239 T1057 T1058 advmod directly,from
R240 T1058 T1050 conj from,by
R241 T1059 T1058 pobj blastocysts,from
R242 T1060 T1059 acl derived,blastocysts
R243 T1061 T1060 prep from,derived
R244 T1062 T1063 amod heterozygous,intercrosses
R245 T1063 T1061 pobj intercrosses,from
R246 T1064 T1041 punct .,generated
R247 T1066 T1067 nmod Northern,blot
R248 T1067 T1068 nmod blot,analyses
R249 T1068 T1072 nsubj analyses,confirmed
R250 T1069 T1067 cc and,blot
R251 T1070 T1071 compound western,blot
R252 T1071 T1067 conj blot,blot
R253 T1073 T1074 det the,absence
R254 T1074 T1072 dobj absence,confirmed
R255 T1075 T1074 prep of,absence
R256 T1076 T1075 pobj ESG1,of
R257 T1077 T1074 prep in,absence
R258 T1078 T1079 det these,cells
R259 T1079 T1077 pobj cells,in
R260 T1080 T1072 punct .,confirmed
R261 T1082 T1083 det These,cells
R262 T1083 T1085 nsubj cells,demonstrated
R263 T1084 T1083 compound ES,cells
R264 T1086 T1087 amod normal,morphology
R265 T1087 T1085 dobj morphology,demonstrated
R266 T1088 T1087 punct ", ",morphology
R267 T1089 T1087 conj proliferation,morphology
R268 T1090 T1089 punct ", ",proliferation
R269 T1091 T1089 cc and,proliferation
R270 T1092 T1089 conj differentiation,proliferation
R271 T1093 T1085 punct .,demonstrated
R89 T894 T895 det A,search
R90 T895 T897 nsubj search,failed
R91 T896 T895 compound Blast,search
R92 T898 T895 prep of,search
R93 T899 T900 nmod mouse,databases
R94 T900 T898 pobj databases,of
R95 T901 T900 amod genomic,databases
R96 T902 T903 aux to,locate
R97 T903 T897 xcomp locate,failed
R98 T904 T905 det the,gene
R99 T905 T903 dobj gene,locate