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PMC:1420271 / 14435-16392 JSONTXT 4 Projects

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Id Subject Object Predicate Lexical cue
T8264 0-14 NN denotes Identification
T8265 15-18 CC denotes and
T8266 19-27 NNS denotes analyses
T8267 28-30 IN denotes of
T8268 31-34 NN denotes BAC
T8269 35-41 NNS denotes clones
T8270 42-52 VBG denotes containing
T8271 53-56 DT denotes the
T8273 57-62 NN denotes mouse
T8274 63-67 NN denotes ESG1
T8272 68-72 NN denotes gene
T8275 72-342 sentence denotes To identify bacterial artificial chromosome (BAC) clones containing mouse ESG1 gene, we performed PCR-based screening of mouse BAC library DNA pools (Research Genetics) using the pH34-u38 (5'-GAAGTCTGGTTCCTTGGCAGG-3') and pH34-L394 (5'-ACTCGATACACTGGCCTAGC-3') primers.
T8276 73-75 TO denotes To
T8277 76-84 VB denotes identify
T8279 85-94 JJ denotes bacterial
T8281 95-105 JJ denotes artificial
T8280 106-116 NN denotes chromosome
T8283 117-118 -LRB- denotes (
T8284 118-121 NN denotes BAC
T8285 121-122 -RRB- denotes )
T8282 123-129 NNS denotes clones
T8286 130-140 VBG denotes containing
T8287 141-146 NN denotes mouse
T8289 147-151 NN denotes ESG1
T8288 152-156 NN denotes gene
T8290 156-158 , denotes ,
T8291 158-160 PRP denotes we
T8278 161-170 VBD denotes performed
T8292 171-174 NN denotes PCR
T8294 174-175 HYPH denotes -
T8293 175-180 VBN denotes based
T8295 181-190 NN denotes screening
T8296 191-193 IN denotes of
T8297 194-199 NN denotes mouse
T8299 200-203 NN denotes BAC
T8300 204-211 NN denotes library
T8301 212-215 NN denotes DNA
T8298 216-221 NNS denotes pools
T8302 222-223 -LRB- denotes (
T8304 223-231 NNP denotes Research
T8303 232-240 NNP denotes Genetics
T8305 240-241 -RRB- denotes )
T8306 242-247 VBG denotes using
T8307 248-251 DT denotes the
T8309 252-256 NN denotes pH34
T8311 256-257 HYPH denotes -
T8310 257-260 NN denotes u38
T8312 261-262 -LRB- denotes (
T8314 262-263 CD denotes 5
T8315 263-264 SYM denotes '
T8316 264-265 HYPH denotes -
T8317 265-286 NN denotes GAAGTCTGGTTCCTTGGCAGG
T8318 286-287 HYPH denotes -
T8313 287-288 CD denotes 3
T8319 288-289 SYM denotes '
T8320 289-290 -RRB- denotes )
T8321 291-294 CC denotes and
T8322 295-299 NN denotes pH34
T8324 299-300 HYPH denotes -
T8323 300-304 NN denotes L394
T8325 305-306 -LRB- denotes (
T8327 306-307 CD denotes 5
T8328 307-308 SYM denotes '
T8329 308-309 HYPH denotes -
T8330 309-329 NN denotes ACTCGATACACTGGCCTAGC
T8331 329-330 HYPH denotes -
T8326 330-331 CD denotes 3
T8332 331-332 SYM denotes '
T8333 332-333 -RRB- denotes )
T8308 334-341 NNS denotes primers
T8334 341-342 . denotes .
T8335 342-698 sentence denotes Following restriction enzyme digestion, we performed Southern blot analyses of BAC clones as described [20] using the pH34-U258 (5'-CTCGAGTGTACAGTCAAGTGGTTGCTGGGA-3'), pH34-U65 (5'-GTGACCCTCGTGACCCGTAA-3'), pH34-intron1L (5'-CTGCGTGAGAGAAACACCAAACAGGC-3'), pH34-L545 (5'-TGTGAATGGGAAGGTTACCACTCT-3') and pH34-SCL1 (5'-GCCCTCTTCTGGTTTGTCTCGAAAT-3') probes.
T8336 343-352 VBG denotes Following
T8338 353-364 NN denotes restriction
T8339 365-371 NN denotes enzyme
T8340 372-381 NN denotes digestion
T8341 381-383 , denotes ,
T8342 383-385 PRP denotes we
T8337 386-395 VBD denotes performed
T8343 396-404 NNP denotes Southern
T8344 405-409 NN denotes blot
T8345 410-418 NNS denotes analyses
T8346 419-421 IN denotes of
T8347 422-425 NN denotes BAC
T8348 426-432 NNS denotes clones
T8349 433-435 IN denotes as
T8350 436-445 VBN denotes described
T8351 446-447 -LRB- denotes [
T8352 447-449 CD denotes 20
T8353 449-450 -RRB- denotes ]
T8354 451-456 VBG denotes using
T8355 457-460 DT denotes the
T8357 461-465 NN denotes pH34
T8359 465-466 HYPH denotes -
T8358 466-470 NN denotes U258
T8360 471-472 -LRB- denotes (
T8362 472-473 CD denotes 5
T8363 473-474 SYM denotes '
T8364 474-475 HYPH denotes -
T8361 475-505 NN denotes CTCGAGTGTACAGTCAAGTGGTTGCTGGGA
T8365 505-506 HYPH denotes -
T8366 506-507 CD denotes 3
T8367 507-508 SYM denotes '
T8368 508-509 -RRB- denotes )
T8369 509-511 , denotes ,
T8370 511-515 NN denotes pH34
T8372 515-516 HYPH denotes -
T8371 516-519 NN denotes U65
T8373 520-521 -LRB- denotes (
T8375 521-522 CD denotes 5
T8376 522-523 SYM denotes '
T8377 523-524 HYPH denotes -
T8374 524-544 NN denotes GTGACCCTCGTGACCCGTAA
T8378 544-545 HYPH denotes -
T8379 545-546 CD denotes 3
T8380 546-547 SYM denotes '
T8381 547-548 -RRB- denotes )
T8382 548-550 , denotes ,
T8383 550-554 NN denotes pH34
T8385 554-555 HYPH denotes -
T8384 555-563 NN denotes intron1L
T8386 564-565 -LRB- denotes (
T8388 565-566 CD denotes 5
T8389 566-567 SYM denotes '
T8390 567-568 HYPH denotes -
T8387 568-594 NN denotes CTGCGTGAGAGAAACACCAAACAGGC
T8391 594-595 HYPH denotes -
T8392 595-596 CD denotes 3
T8393 596-597 SYM denotes '
T8394 597-598 -RRB- denotes )
T8395 598-600 , denotes ,
T8396 600-604 NN denotes pH34
T8398 604-605 HYPH denotes -
T8397 605-609 NN denotes L545
T8399 610-611 -LRB- denotes (
T8401 611-612 CD denotes 5
T8402 612-613 SYM denotes '
T8403 613-614 HYPH denotes -
T8400 614-638 NN denotes TGTGAATGGGAAGGTTACCACTCT
T8404 638-639 HYPH denotes -
T8405 639-640 CD denotes 3
T8406 640-641 SYM denotes '
T8407 641-642 -RRB- denotes )
T8408 643-646 CC denotes and
T8409 647-651 NN denotes pH34
T8411 651-652 HYPH denotes -
T8410 652-656 NN denotes SCL1
T8412 657-658 -LRB- denotes (
T8414 658-659 CD denotes 5
T8415 659-660 SYM denotes '
T8416 660-661 HYPH denotes -
T8413 661-686 NN denotes GCCCTCTTCTGGTTTGTCTCGAAAT
T8417 686-687 HYPH denotes -
T8418 687-688 CD denotes 3
T8419 688-689 SYM denotes '
T8420 689-690 -RRB- denotes )
T8356 691-697 NNS denotes probes
T8421 697-698 . denotes .
T8422 698-793 sentence denotes Hybridization with these probes revealed bands containing either the ESG1 gene or pseudogenes.
T8423 699-712 NN denotes Hybridization
T8425 713-717 IN denotes with
T8426 718-723 DT denotes these
T8427 724-730 NNS denotes probes
T8424 731-739 VBD denotes revealed
T8428 740-745 NNS denotes bands
T8429 746-756 VBG denotes containing
T8430 757-763 CC denotes either
T8432 764-767 DT denotes the
T8433 768-772 NN denotes ESG1
T8431 773-777 NN denotes gene
T8434 778-780 CC denotes or
T8435 781-792 NNS denotes pseudogenes
T8436 792-793 . denotes .
T8437 793-955 sentence denotes To sequence the region containing the mouse ESG1 gene and the 3' flanking region, we subcloned a ~15 kbp XhoI-SalI fragment into the pZERO-2 vector (Invitrogen).
T8438 794-796 TO denotes To
T8439 797-805 VB denotes sequence
T8441 806-809 DT denotes the
T8442 810-816 NN denotes region
T8443 817-827 VBG denotes containing
T8444 828-831 DT denotes the
T8446 832-837 NN denotes mouse
T8447 838-842 NN denotes ESG1
T8445 843-847 NN denotes gene
T8448 848-851 CC denotes and
T8449 852-855 DT denotes the
T8451 856-857 CD denotes 3
T8452 857-858 SYM denotes '
T8453 859-867 NN denotes flanking
T8450 868-874 NN denotes region
T8454 874-876 , denotes ,
T8455 876-878 PRP denotes we
T8440 879-888 VBD denotes subcloned
T8456 889-890 DT denotes a
T8458 891-892 SYM denotes ~
T8459 892-894 CD denotes 15
T8460 895-898 NN denotes kbp
T8461 899-903 NN denotes XhoI
T8463 903-904 HYPH denotes -
T8462 904-908 NN denotes SalI
T8457 909-917 NN denotes fragment
T8464 918-922 IN denotes into
T8465 923-926 DT denotes the
T8467 927-932 NN denotes pZERO
T8468 932-933 HYPH denotes -
T8469 933-934 CD denotes 2
T8466 935-941 NN denotes vector
T8470 942-943 -LRB- denotes (
T8471 943-953 NNP denotes Invitrogen
T8472 953-954 -RRB- denotes )
T8473 954-955 . denotes .
T8474 955-1063 sentence denotes HindIII- or EcoRI-digested fragments of this vector were then cloned into pBluescript KS(-) for sequencing.
T8475 956-963 NN denotes HindIII
T8477 963-964 HYPH denotes -
T8478 965-967 CC denotes or
T8479 968-973 NN denotes EcoRI
T8480 973-974 HYPH denotes -
T8476 974-982 VBN denotes digested
T8481 983-992 NNS denotes fragments
T8483 993-995 IN denotes of
T8484 996-1000 DT denotes this
T8485 1001-1007 NN denotes vector
T8486 1008-1012 VBD denotes were
T8487 1013-1017 RB denotes then
T8482 1018-1024 VBN denotes cloned
T8488 1025-1029 IN denotes into
T8489 1030-1041 NN denotes pBluescript
T8490 1042-1044 NN denotes KS
T8491 1044-1045 -LRB- denotes (
T8492 1045-1046 SYM denotes -
T8493 1046-1047 -RRB- denotes )
T8494 1048-1051 IN denotes for
T8495 1052-1062 NN denotes sequencing
T8496 1062-1063 . denotes .
T8497 1063-1186 sentence denotes To sequence the ESG1 pseudogene and the 3' flanking region, an 8 kbp NotI/XhoI fragment was cloned into pBluescript KS(-).
T8498 1064-1066 TO denotes To
T8499 1067-1075 VB denotes sequence
T8501 1076-1079 DT denotes the
T8503 1080-1084 NN denotes ESG1
T8502 1085-1095 NN denotes pseudogene
T8504 1096-1099 CC denotes and
T8505 1100-1103 DT denotes the
T8507 1104-1105 CD denotes 3
T8508 1105-1106 SYM denotes '
T8509 1107-1115 NN denotes flanking
T8506 1116-1122 NN denotes region
T8510 1122-1124 , denotes ,
T8511 1124-1126 DT denotes an
T8513 1127-1128 CD denotes 8
T8514 1129-1132 NN denotes kbp
T8515 1133-1137 NN denotes NotI
T8517 1137-1138 HYPH denotes /
T8516 1138-1142 NN denotes XhoI
T8512 1143-1151 NN denotes fragment
T8518 1152-1155 VBD denotes was
T8500 1156-1162 VBN denotes cloned
T8519 1163-1167 IN denotes into
T8520 1168-1179 NN denotes pBluescript
T8521 1180-1182 NN denotes KS
T8522 1182-1183 -LRB- denotes (
T8523 1183-1184 SYM denotes -
T8524 1184-1185 -RRB- denotes )
T8525 1185-1186 . denotes .
T8526 1186-1268 sentence denotes BamHI- or PstI- fragments of this vector were also cloned into pBluescript KS(-).
T8527 1187-1192 NN denotes BamHI
T8529 1192-1193 SYM denotes -
T8530 1194-1196 CC denotes or
T8531 1197-1201 NN denotes PstI
T8532 1201-1202 SYM denotes -
T8528 1203-1212 NNS denotes fragments
T8534 1213-1215 IN denotes of
T8535 1216-1220 DT denotes this
T8536 1221-1227 NN denotes vector
T8537 1228-1232 VBD denotes were
T8538 1233-1237 RB denotes also
T8533 1238-1244 VBN denotes cloned
T8539 1245-1249 IN denotes into
T8540 1250-1261 NN denotes pBluescript
T8541 1262-1264 NN denotes KS
T8542 1264-1265 -LRB- denotes (
T8543 1265-1266 SYM denotes -
T8544 1266-1267 -RRB- denotes )
T8545 1267-1268 . denotes .
T8546 1268-1493 sentence denotes To identify the sequence containing the 5' flanking regions of the ESG1 gene and the related pseudogenes, we used a TOPO walker kit (Invitrogen) with the pH34-T2L (5'-ACTAGTCGCAGCAGGGATCCAGGAATATCT-3') and pH34-L394 primers.
T8547 1269-1271 TO denotes To
T8548 1272-1280 VB denotes identify
T8550 1281-1284 DT denotes the
T8551 1285-1293 NN denotes sequence
T8552 1294-1304 VBG denotes containing
T8553 1305-1308 DT denotes the
T8555 1309-1310 CD denotes 5
T8556 1310-1311 SYM denotes '
T8557 1312-1320 NN denotes flanking
T8554 1321-1328 NNS denotes regions
T8558 1329-1331 IN denotes of
T8559 1332-1335 DT denotes the
T8561 1336-1340 NN denotes ESG1
T8560 1341-1345 NN denotes gene
T8562 1346-1349 CC denotes and
T8563 1350-1353 DT denotes the
T8565 1354-1361 JJ denotes related
T8564 1362-1373 NNS denotes pseudogenes
T8566 1373-1375 , denotes ,
T8567 1375-1377 PRP denotes we
T8549 1378-1382 VBD denotes used
T8568 1383-1384 DT denotes a
T8570 1385-1389 NN denotes TOPO
T8571 1390-1396 NN denotes walker
T8569 1397-1400 NN denotes kit
T8572 1401-1402 -LRB- denotes (
T8573 1402-1412 NNP denotes Invitrogen
T8574 1412-1413 -RRB- denotes )
T8575 1414-1418 IN denotes with
T8576 1419-1422 DT denotes the
T8578 1423-1427 NN denotes pH34
T8580 1427-1428 HYPH denotes -
T8579 1428-1431 NN denotes T2L
T8581 1432-1433 -LRB- denotes (
T8583 1433-1434 CD denotes 5
T8584 1434-1435 SYM denotes '
T8585 1435-1436 HYPH denotes -
T8586 1436-1466 NN denotes ACTAGTCGCAGCAGGGATCCAGGAATATCT
T8587 1466-1467 HYPH denotes -
T8582 1467-1468 CD denotes 3
T8588 1468-1469 SYM denotes '
T8589 1469-1470 -RRB- denotes )
T8590 1471-1474 CC denotes and
T8591 1475-1479 NN denotes pH34
T8593 1479-1480 HYPH denotes -
T8592 1480-1484 NN denotes L394
T8577 1485-1492 NNS denotes primers
T8594 1492-1493 . denotes .
T8595 1493-1553 sentence denotes The resulting sequence was cloned into pCR2.1 (Invitrogen).
T8596 1494-1497 DT denotes The
T8598 1498-1507 VBG denotes resulting
T8597 1508-1516 NN denotes sequence
T8600 1517-1520 VBD denotes was
T8599 1521-1527 VBN denotes cloned
T8601 1528-1532 IN denotes into
T8602 1533-1539 NN denotes pCR2.1
T8603 1540-1541 -LRB- denotes (
T8604 1541-1551 NNP denotes Invitrogen
T8605 1551-1552 -RRB- denotes )
T8606 1552-1553 . denotes .
T8607 1553-1726 sentence denotes We obtained a ~6 kbp band from the NsiI-digensted library; XbaI-, SpeI-, EcoRI-, and PstI-digested fragments of this band were cloned into pBluescript KS(-) for sequencing.
T8608 1554-1556 PRP denotes We
T8609 1557-1565 VBD denotes obtained
T8611 1566-1567 DT denotes a
T8613 1568-1569 SYM denotes ~
T8614 1569-1570 CD denotes 6
T8615 1571-1574 NN denotes kbp
T8612 1575-1579 NN denotes band
T8616 1580-1584 IN denotes from
T8617 1585-1588 DT denotes the
T8619 1589-1593 NN denotes NsiI
T8621 1593-1594 HYPH denotes -
T8620 1594-1603 VBN denotes digensted
T8618 1604-1611 NN denotes library
T8622 1611-1612 : denotes ;
T8623 1613-1617 NN denotes XbaI
T8625 1617-1618 HYPH denotes -
T8626 1618-1620 , denotes ,
T8627 1620-1624 NN denotes SpeI
T8628 1624-1625 HYPH denotes -
T8629 1625-1627 , denotes ,
T8630 1627-1632 NN denotes EcoRI
T8631 1632-1633 HYPH denotes -
T8632 1633-1635 , denotes ,
T8633 1635-1638 CC denotes and
T8634 1639-1643 NN denotes PstI
T8635 1643-1644 HYPH denotes -
T8624 1644-1652 VBN denotes digested
T8636 1653-1662 NNS denotes fragments
T8637 1663-1665 IN denotes of
T8638 1666-1670 DT denotes this
T8639 1671-1675 NN denotes band
T8640 1676-1680 VBD denotes were
T8610 1681-1687 VBN denotes cloned
T8641 1688-1692 IN denotes into
T8642 1693-1704 NN denotes pBluescript
T8643 1705-1707 NN denotes KS
T8644 1707-1708 -LRB- denotes (
T8645 1708-1709 SYM denotes -
T8646 1709-1710 -RRB- denotes )
T8647 1711-1714 IN denotes for
T8648 1715-1725 NN denotes sequencing
T8649 1725-1726 . denotes .
T8650 1726-1791 sentence denotes This fragment contained the 5' flanking region of the ESG1 gene.
T8651 1727-1731 DT denotes This
T8652 1732-1740 NN denotes fragment
T8653 1741-1750 VBD denotes contained
T8654 1751-1754 DT denotes the
T8656 1755-1756 CD denotes 5
T8657 1756-1757 SYM denotes '
T8658 1758-1766 NN denotes flanking
T8655 1767-1773 NN denotes region
T8659 1774-1776 IN denotes of
T8660 1777-1780 DT denotes the
T8662 1781-1785 NN denotes ESG1
T8661 1786-1790 NN denotes gene
T8663 1790-1791 . denotes .
T8664 1791-1890 sentence denotes A ~3 kbp fragment, obtained from the SacI-digested library, was cloned into pCR2.1 for sequencing.
T8665 1792-1793 DT denotes A
T8667 1794-1795 SYM denotes ~
T8668 1795-1796 CD denotes 3
T8669 1797-1800 NN denotes kbp
T8666 1801-1809 NN denotes fragment
T8671 1809-1811 , denotes ,
T8672 1811-1819 VBN denotes obtained
T8673 1820-1824 IN denotes from
T8674 1825-1828 DT denotes the
T8676 1829-1833 NN denotes SacI
T8678 1833-1834 HYPH denotes -
T8677 1834-1842 VBN denotes digested
T8675 1843-1850 NN denotes library
T8679 1850-1852 , denotes ,
T8680 1852-1855 VBD denotes was
T8670 1856-1862 VBN denotes cloned
T8681 1863-1867 IN denotes into
T8682 1868-1874 NN denotes pCR2.1
T8683 1875-1878 IN denotes for
T8684 1879-1889 NN denotes sequencing
T8685 1889-1890 . denotes .
T8686 1890-1957 sentence denotes This fragment was contained the 5' region flanking the pseudogene.
T8687 1891-1895 DT denotes This
T8688 1896-1904 NN denotes fragment
T8690 1905-1908 VBD denotes was
T8689 1909-1918 VBN denotes contained
T8691 1919-1922 DT denotes the
T8693 1923-1924 CD denotes 5
T8694 1924-1925 SYM denotes '
T8692 1926-1932 NN denotes region
T8695 1933-1941 VBG denotes flanking
T8696 1942-1945 DT denotes the
T8697 1946-1956 NN denotes pseudogene
T8698 1956-1957 . denotes .
R2053 T8265 T8264 cc and,Identification
R2054 T8266 T8264 conj analyses,Identification
R2055 T8267 T8264 prep of,Identification
R2056 T8268 T8269 compound BAC,clones
R2057 T8269 T8267 pobj clones,of
R2058 T8270 T8269 acl containing,clones
R2059 T8271 T8272 det the,gene
R2060 T8272 T8270 dobj gene,containing
R2061 T8273 T8272 compound mouse,gene
R2062 T8274 T8272 compound ESG1,gene
R2063 T8276 T8277 aux To,identify
R2064 T8277 T8278 advcl identify,performed
R2065 T8279 T8280 amod bacterial,chromosome
R2066 T8280 T8282 nmod chromosome,clones
R2067 T8281 T8280 amod artificial,chromosome
R2068 T8282 T8277 dobj clones,identify
R2069 T8283 T8280 punct (,chromosome
R2070 T8284 T8280 appos BAC,chromosome
R2071 T8285 T8282 punct ),clones
R2072 T8286 T8282 acl containing,clones
R2073 T8287 T8288 compound mouse,gene
R2074 T8288 T8286 dobj gene,containing
R2075 T8289 T8288 compound ESG1,gene
R2076 T8290 T8278 punct ", ",performed
R2077 T8291 T8278 nsubj we,performed
R2078 T8292 T8293 npadvmod PCR,based
R2079 T8293 T8295 amod based,screening
R2080 T8294 T8293 punct -,based
R2081 T8295 T8278 dobj screening,performed
R2082 T8296 T8295 prep of,screening
R2083 T8297 T8298 compound mouse,pools
R2084 T8298 T8296 pobj pools,of
R2085 T8299 T8298 compound BAC,pools
R2086 T8300 T8298 compound library,pools
R2087 T8301 T8298 compound DNA,pools
R2088 T8302 T8303 punct (,Genetics
R2089 T8303 T8295 parataxis Genetics,screening
R2090 T8304 T8303 compound Research,Genetics
R2091 T8305 T8303 punct ),Genetics
R2092 T8306 T8278 advcl using,performed
R2093 T8307 T8308 det the,primers
R2094 T8308 T8306 dobj primers,using
R2095 T8309 T8310 nmod pH34,u38
R2096 T8310 T8308 nmod u38,primers
R2097 T8311 T8310 punct -,u38
R2098 T8312 T8313 punct (,3
R2099 T8313 T8310 parataxis 3,u38
R2100 T8314 T8313 dep 5,3
R2101 T8315 T8314 punct ',5
R2102 T8316 T8313 punct -,3
R2103 T8317 T8313 dep GAAGTCTGGTTCCTTGGCAGG,3
R2104 T8318 T8313 punct -,3
R2105 T8319 T8313 punct ',3
R2106 T8320 T8313 punct ),3
R2107 T8321 T8310 cc and,u38
R2108 T8322 T8323 compound pH34,L394
R2109 T8323 T8310 conj L394,u38
R2110 T8324 T8323 punct -,L394
R2111 T8325 T8326 punct (,3
R2112 T8326 T8323 parataxis 3,L394
R2113 T8327 T8326 dep 5,3
R2114 T8328 T8327 punct ',5
R2115 T8329 T8326 punct -,3
R2116 T8330 T8326 dep ACTCGATACACTGGCCTAGC,3
R2117 T8331 T8326 punct -,3
R2118 T8332 T8326 punct ',3
R2119 T8333 T8326 punct ),3
R2120 T8334 T8278 punct .,performed
R2121 T8336 T8337 prep Following,performed
R2122 T8338 T8339 compound restriction,enzyme
R2123 T8339 T8340 compound enzyme,digestion
R2124 T8340 T8336 pobj digestion,Following
R2125 T8341 T8337 punct ", ",performed
R2126 T8342 T8337 nsubj we,performed
R2127 T8343 T8344 compound Southern,blot
R2128 T8344 T8345 compound blot,analyses
R2129 T8345 T8337 dobj analyses,performed
R2130 T8346 T8345 prep of,analyses
R2131 T8347 T8348 compound BAC,clones
R2132 T8348 T8346 pobj clones,of
R2133 T8349 T8350 mark as,described
R2134 T8350 T8337 advcl described,performed
R2135 T8351 T8352 punct [,20
R2136 T8352 T8350 parataxis 20,described
R2137 T8353 T8352 punct ],20
R2138 T8354 T8350 advcl using,described
R2139 T8355 T8356 det the,probes
R2140 T8356 T8354 dobj probes,using
R2141 T8357 T8358 nmod pH34,U258
R2142 T8358 T8356 nmod U258,probes
R2143 T8359 T8358 punct -,U258
R2144 T8360 T8361 punct (,CTCGAGTGTACAGTCAAGTGGTTGCTGGGA
R2145 T8361 T8358 parataxis CTCGAGTGTACAGTCAAGTGGTTGCTGGGA,U258
R2146 T8362 T8361 nummod 5,CTCGAGTGTACAGTCAAGTGGTTGCTGGGA
R2147 T8363 T8362 punct ',5
R2148 T8364 T8361 punct -,CTCGAGTGTACAGTCAAGTGGTTGCTGGGA
R2149 T8365 T8361 punct -,CTCGAGTGTACAGTCAAGTGGTTGCTGGGA
R2150 T8366 T8361 nummod 3,CTCGAGTGTACAGTCAAGTGGTTGCTGGGA
R2151 T8367 T8361 punct ',CTCGAGTGTACAGTCAAGTGGTTGCTGGGA
R2152 T8368 T8361 punct ),CTCGAGTGTACAGTCAAGTGGTTGCTGGGA
R2153 T8369 T8358 punct ", ",U258
R2154 T8370 T8371 compound pH34,U65
R2155 T8371 T8358 conj U65,U258
R2156 T8372 T8371 punct -,U65
R2157 T8373 T8374 punct (,GTGACCCTCGTGACCCGTAA
R2158 T8374 T8371 parataxis GTGACCCTCGTGACCCGTAA,U65
R2159 T8375 T8374 nummod 5,GTGACCCTCGTGACCCGTAA
R2160 T8376 T8375 punct ',5
R2161 T8377 T8374 punct -,GTGACCCTCGTGACCCGTAA
R2162 T8378 T8374 punct -,GTGACCCTCGTGACCCGTAA
R2163 T8379 T8374 nummod 3,GTGACCCTCGTGACCCGTAA
R2164 T8380 T8374 punct ',GTGACCCTCGTGACCCGTAA
R2165 T8381 T8374 punct ),GTGACCCTCGTGACCCGTAA
R2166 T8382 T8371 punct ", ",U65
R2167 T8383 T8384 compound pH34,intron1L
R2168 T8384 T8371 conj intron1L,U65
R2169 T8385 T8384 punct -,intron1L
R2170 T8386 T8387 punct (,CTGCGTGAGAGAAACACCAAACAGGC
R2171 T8387 T8384 parataxis CTGCGTGAGAGAAACACCAAACAGGC,intron1L
R2172 T8388 T8387 nummod 5,CTGCGTGAGAGAAACACCAAACAGGC
R2173 T8389 T8388 punct ',5
R2174 T8390 T8387 punct -,CTGCGTGAGAGAAACACCAAACAGGC
R2175 T8391 T8387 punct -,CTGCGTGAGAGAAACACCAAACAGGC
R2176 T8392 T8387 nummod 3,CTGCGTGAGAGAAACACCAAACAGGC
R2177 T8393 T8387 punct ',CTGCGTGAGAGAAACACCAAACAGGC
R2178 T8394 T8387 punct ),CTGCGTGAGAGAAACACCAAACAGGC
R2179 T8395 T8384 punct ", ",intron1L
R2180 T8396 T8397 compound pH34,L545
R2181 T8397 T8384 conj L545,intron1L
R2182 T8398 T8397 punct -,L545
R2183 T8399 T8400 punct (,TGTGAATGGGAAGGTTACCACTCT
R2184 T8400 T8397 parataxis TGTGAATGGGAAGGTTACCACTCT,L545
R2185 T8401 T8400 nummod 5,TGTGAATGGGAAGGTTACCACTCT
R2186 T8402 T8401 punct ',5
R2187 T8403 T8400 punct -,TGTGAATGGGAAGGTTACCACTCT
R2188 T8404 T8400 punct -,TGTGAATGGGAAGGTTACCACTCT
R2189 T8405 T8400 nummod 3,TGTGAATGGGAAGGTTACCACTCT
R2190 T8406 T8400 punct ',TGTGAATGGGAAGGTTACCACTCT
R2191 T8407 T8400 punct ),TGTGAATGGGAAGGTTACCACTCT
R2192 T8408 T8397 cc and,L545
R2193 T8409 T8410 compound pH34,SCL1
R2194 T8410 T8397 conj SCL1,L545
R2195 T8411 T8410 punct -,SCL1
R2196 T8412 T8413 punct (,GCCCTCTTCTGGTTTGTCTCGAAAT
R2197 T8413 T8410 parataxis GCCCTCTTCTGGTTTGTCTCGAAAT,SCL1
R2198 T8414 T8413 nummod 5,GCCCTCTTCTGGTTTGTCTCGAAAT
R2199 T8415 T8414 punct ',5
R2200 T8416 T8413 punct -,GCCCTCTTCTGGTTTGTCTCGAAAT
R2201 T8417 T8413 punct -,GCCCTCTTCTGGTTTGTCTCGAAAT
R2202 T8418 T8413 nummod 3,GCCCTCTTCTGGTTTGTCTCGAAAT
R2203 T8419 T8413 punct ',GCCCTCTTCTGGTTTGTCTCGAAAT
R2204 T8420 T8413 punct ),GCCCTCTTCTGGTTTGTCTCGAAAT
R2205 T8421 T8337 punct .,performed
R2206 T8423 T8424 nsubj Hybridization,revealed
R2207 T8425 T8423 prep with,Hybridization
R2208 T8426 T8427 det these,probes
R2209 T8427 T8425 pobj probes,with
R2210 T8428 T8424 dobj bands,revealed
R2211 T8429 T8428 acl containing,bands
R2212 T8430 T8431 preconj either,gene
R2213 T8431 T8429 dobj gene,containing
R2214 T8432 T8431 det the,gene
R2215 T8433 T8431 compound ESG1,gene
R2216 T8434 T8431 cc or,gene
R2217 T8435 T8431 conj pseudogenes,gene
R2218 T8436 T8424 punct .,revealed
R2219 T8438 T8439 aux To,sequence
R2220 T8439 T8440 advcl sequence,subcloned
R2221 T8441 T8442 det the,region
R2222 T8442 T8439 dobj region,sequence
R2223 T8443 T8442 acl containing,region
R2224 T8444 T8445 det the,gene
R2225 T8445 T8443 dobj gene,containing
R2226 T8446 T8445 compound mouse,gene
R2227 T8447 T8445 compound ESG1,gene
R2228 T8448 T8445 cc and,gene
R2229 T8449 T8450 det the,region
R2230 T8450 T8445 conj region,gene
R2231 T8451 T8450 nummod 3,region
R2232 T8452 T8451 punct ',3
R2233 T8453 T8450 compound flanking,region
R2234 T8454 T8440 punct ", ",subcloned
R2235 T8455 T8440 nsubj we,subcloned
R2236 T8456 T8457 det a,fragment
R2237 T8457 T8440 dobj fragment,subcloned
R2238 T8458 T8459 punct ~,15
R2239 T8459 T8460 nummod 15,kbp
R2240 T8460 T8457 compound kbp,fragment
R2241 T8461 T8462 compound XhoI,SalI
R2242 T8462 T8457 compound SalI,fragment
R2243 T8463 T8462 punct -,SalI
R2244 T8464 T8440 prep into,subcloned
R2245 T8465 T8466 det the,vector
R2246 T8466 T8464 pobj vector,into
R2247 T8467 T8466 nmod pZERO,vector
R2248 T8468 T8467 punct -,pZERO
R2249 T8469 T8467 nummod 2,pZERO
R2250 T8470 T8471 punct (,Invitrogen
R2251 T8471 T8466 parataxis Invitrogen,vector
R2252 T8472 T8471 punct ),Invitrogen
R2253 T8473 T8440 punct .,subcloned
R2254 T8475 T8476 npadvmod HindIII,digested
R2255 T8476 T8481 amod digested,fragments
R2256 T8477 T8475 punct -,HindIII
R2257 T8478 T8475 cc or,HindIII
R2258 T8479 T8475 conj EcoRI,HindIII
R2259 T8480 T8476 punct -,digested
R2260 T8481 T8482 nsubjpass fragments,cloned
R2261 T8483 T8481 prep of,fragments
R2262 T8484 T8485 det this,vector
R2263 T8485 T8483 pobj vector,of
R2264 T8486 T8482 auxpass were,cloned
R2265 T8487 T8482 advmod then,cloned
R2266 T8488 T8482 prep into,cloned
R2267 T8489 T8490 compound pBluescript,KS
R2268 T8490 T8488 pobj KS,into
R2269 T8491 T8490 punct (,KS
R2270 T8492 T8490 punct -,KS
R2271 T8493 T8490 punct ),KS
R2272 T8494 T8482 prep for,cloned
R2273 T8495 T8494 pobj sequencing,for
R2274 T8496 T8482 punct .,cloned
R2275 T8498 T8499 aux To,sequence
R2276 T8499 T8500 advcl sequence,cloned
R2277 T8501 T8502 det the,pseudogene
R2278 T8502 T8499 dobj pseudogene,sequence
R2279 T8503 T8502 compound ESG1,pseudogene
R2280 T8504 T8502 cc and,pseudogene
R2281 T8505 T8506 det the,region
R2282 T8506 T8502 conj region,pseudogene
R2283 T8507 T8506 nummod 3,region
R2284 T8508 T8507 punct ',3
R2285 T8509 T8506 compound flanking,region
R2286 T8510 T8500 punct ", ",cloned
R2287 T8511 T8512 det an,fragment
R2288 T8512 T8500 nsubjpass fragment,cloned
R2289 T8513 T8514 nummod 8,kbp
R2290 T8514 T8512 compound kbp,fragment
R2291 T8515 T8516 compound NotI,XhoI
R2292 T8516 T8512 compound XhoI,fragment
R2293 T8517 T8516 punct /,XhoI
R2294 T8518 T8500 auxpass was,cloned
R2295 T8519 T8500 prep into,cloned
R2296 T8520 T8521 compound pBluescript,KS
R2297 T8521 T8519 pobj KS,into
R2298 T8522 T8521 punct (,KS
R2299 T8523 T8521 punct -,KS
R2300 T8524 T8521 punct ),KS
R2301 T8525 T8500 punct .,cloned
R2302 T8527 T8528 nmod BamHI,fragments
R2303 T8528 T8533 nsubjpass fragments,cloned
R2304 T8529 T8527 punct -,BamHI
R2305 T8530 T8527 cc or,BamHI
R2306 T8531 T8527 conj PstI,BamHI
R2307 T8532 T8528 punct -,fragments
R2308 T8534 T8528 prep of,fragments
R2309 T8535 T8536 det this,vector
R2310 T8536 T8534 pobj vector,of
R2311 T8537 T8533 auxpass were,cloned
R2312 T8538 T8533 advmod also,cloned
R2313 T8539 T8533 prep into,cloned
R2314 T8540 T8541 compound pBluescript,KS
R2315 T8541 T8539 pobj KS,into
R2316 T8542 T8541 punct (,KS
R2317 T8543 T8541 punct -,KS
R2318 T8544 T8541 punct ),KS
R2319 T8545 T8533 punct .,cloned
R2320 T8547 T8548 aux To,identify
R2321 T8548 T8549 advcl identify,used
R2322 T8550 T8551 det the,sequence
R2323 T8551 T8548 dobj sequence,identify
R2324 T8552 T8551 acl containing,sequence
R2325 T8553 T8554 det the,regions
R2326 T8554 T8552 dobj regions,containing
R2327 T8555 T8554 nummod 5,regions
R2328 T8556 T8555 punct ',5
R2329 T8557 T8554 compound flanking,regions
R2330 T8558 T8554 prep of,regions
R2331 T8559 T8560 det the,gene
R2332 T8560 T8558 pobj gene,of
R2333 T8561 T8560 compound ESG1,gene
R2334 T8562 T8560 cc and,gene
R2335 T8563 T8564 det the,pseudogenes
R2336 T8564 T8560 conj pseudogenes,gene
R2337 T8565 T8564 amod related,pseudogenes
R2338 T8566 T8549 punct ", ",used
R2339 T8567 T8549 nsubj we,used
R2340 T8568 T8569 det a,kit
R2341 T8569 T8549 dobj kit,used
R2342 T8570 T8569 compound TOPO,kit
R2343 T8571 T8569 compound walker,kit
R2344 T8572 T8573 punct (,Invitrogen
R2345 T8573 T8569 parataxis Invitrogen,kit
R2346 T8574 T8573 punct ),Invitrogen
R2347 T8575 T8549 prep with,used
R2348 T8576 T8577 det the,primers
R2349 T8577 T8575 pobj primers,with
R2350 T8578 T8579 nmod pH34,T2L
R2351 T8579 T8577 nmod T2L,primers
R2352 T8580 T8579 punct -,T2L
R2353 T8581 T8582 punct (,3
R2354 T8582 T8579 parataxis 3,T2L
R2355 T8583 T8582 dep 5,3
R2356 T8584 T8583 punct ',5
R2357 T8585 T8582 punct -,3
R2358 T8586 T8582 dep ACTAGTCGCAGCAGGGATCCAGGAATATCT,3
R2359 T8587 T8582 punct -,3
R2360 T8588 T8582 punct ',3
R2361 T8589 T8582 punct ),3
R2362 T8590 T8579 cc and,T2L
R2363 T8591 T8592 compound pH34,L394
R2364 T8592 T8579 conj L394,T2L
R2365 T8593 T8592 punct -,L394
R2366 T8594 T8549 punct .,used
R2367 T8596 T8597 det The,sequence
R2368 T8597 T8599 nsubjpass sequence,cloned
R2369 T8598 T8597 amod resulting,sequence
R2370 T8600 T8599 auxpass was,cloned
R2371 T8601 T8599 prep into,cloned
R2372 T8602 T8601 pobj pCR2.1,into
R2373 T8603 T8604 punct (,Invitrogen
R2374 T8604 T8602 parataxis Invitrogen,pCR2.1
R2375 T8605 T8604 punct ),Invitrogen
R2376 T8606 T8599 punct .,cloned
R2377 T8608 T8609 nsubj We,obtained
R2378 T8609 T8610 ccomp obtained,cloned
R2379 T8611 T8612 det a,band
R2380 T8612 T8609 dobj band,obtained
R2381 T8613 T8614 punct ~,6
R2382 T8614 T8615 nummod 6,kbp
R2383 T8615 T8612 compound kbp,band
R2384 T8616 T8609 prep from,obtained
R2385 T8617 T8618 det the,library
R2386 T8618 T8616 pobj library,from
R2387 T8619 T8620 npadvmod NsiI,digensted
R2388 T8620 T8618 amod digensted,library
R2389 T8621 T8620 punct -,digensted
R2390 T8622 T8610 punct ;,cloned
R2391 T8623 T8624 npadvmod XbaI,digested
R2392 T8624 T8636 amod digested,fragments
R2393 T8625 T8623 punct -,XbaI
R2394 T8626 T8623 punct ", ",XbaI
R2395 T8627 T8623 conj SpeI,XbaI
R2396 T8628 T8627 punct -,SpeI
R2397 T8629 T8627 punct ", ",SpeI
R2398 T8630 T8627 conj EcoRI,SpeI
R2399 T8631 T8630 punct -,EcoRI
R2400 T8632 T8630 punct ", ",EcoRI
R2401 T8633 T8630 cc and,EcoRI
R2402 T8634 T8630 conj PstI,EcoRI
R2403 T8635 T8624 punct -,digested
R2404 T8636 T8610 nsubjpass fragments,cloned
R2405 T8637 T8636 prep of,fragments
R2406 T8638 T8639 det this,band
R2407 T8639 T8637 pobj band,of
R2408 T8640 T8610 auxpass were,cloned
R2409 T8641 T8610 prep into,cloned
R2410 T8642 T8643 compound pBluescript,KS
R2411 T8643 T8641 pobj KS,into
R2412 T8644 T8643 punct (,KS
R2413 T8645 T8643 punct -,KS
R2414 T8646 T8643 punct ),KS
R2415 T8647 T8610 prep for,cloned
R2416 T8648 T8647 pobj sequencing,for
R2417 T8649 T8610 punct .,cloned
R2418 T8651 T8652 det This,fragment
R2419 T8652 T8653 nsubj fragment,contained
R2420 T8654 T8655 det the,region
R2421 T8655 T8653 dobj region,contained
R2422 T8656 T8655 nummod 5,region
R2423 T8657 T8656 punct ',5
R2424 T8658 T8655 compound flanking,region
R2425 T8659 T8655 prep of,region
R2426 T8660 T8661 det the,gene
R2427 T8661 T8659 pobj gene,of
R2428 T8662 T8661 compound ESG1,gene
R2429 T8663 T8653 punct .,contained
R2430 T8665 T8666 det A,fragment
R2431 T8666 T8670 nsubjpass fragment,cloned
R2432 T8667 T8668 punct ~,3
R2433 T8668 T8669 nummod 3,kbp
R2434 T8669 T8666 compound kbp,fragment
R2435 T8671 T8666 punct ", ",fragment
R2436 T8672 T8666 acl obtained,fragment
R2437 T8673 T8672 prep from,obtained
R2438 T8674 T8675 det the,library
R2439 T8675 T8673 pobj library,from
R2440 T8676 T8677 npadvmod SacI,digested
R2441 T8677 T8675 amod digested,library
R2442 T8678 T8677 punct -,digested
R2443 T8679 T8670 punct ", ",cloned
R2444 T8680 T8670 auxpass was,cloned
R2445 T8681 T8670 prep into,cloned
R2446 T8682 T8681 pobj pCR2.1,into
R2447 T8683 T8670 prep for,cloned
R2448 T8684 T8683 pobj sequencing,for
R2449 T8685 T8670 punct .,cloned
R2450 T8687 T8688 det This,fragment
R2451 T8688 T8689 nsubjpass fragment,contained
R2452 T8690 T8689 auxpass was,contained
R2453 T8691 T8692 det the,region
R2454 T8692 T8689 dobj region,contained
R2455 T8693 T8692 nummod 5,region
R2456 T8694 T8693 punct ',5
R2457 T8695 T8692 acl flanking,region
R2458 T8696 T8697 det the,pseudogene
R2459 T8697 T8695 dobj pseudogene,flanking
R2460 T8698 T8689 punct .,contained