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PMC:138691 / 3182-4119 JSONTXT 4 Projects

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Id Subject Object Predicate Lexical cue
T1002 0-6 NNP denotes Pierce
T1004 7-9 FW denotes et
T1005 10-12 FW denotes al
T1006 13-17 VBP denotes have
T1003 18-26 VBN denotes designed
T1007 27-28 DT denotes a
T1008 29-32 NN denotes set
T1009 33-35 IN denotes of
T1010 36-49 NN denotes recombination
T1011 50-60 NNS denotes substrates
T1012 61-64 IN denotes for
T1013 65-74 VBG denotes measuring
T1014 75-78 DT denotes the
T1015 79-84 NN denotes level
T1016 85-87 IN denotes of
T1017 88-98 JJ denotes homologous
T1018 99-112 NN denotes recombination
T1019 113-115 FW denotes in
T1020 116-120 FW denotes vivo
T1021 121-122 -LRB- denotes (
T1022 122-126 NN denotes Fig.
T1023 127-128 CD denotes 1
T1024 128-129 -RRB- denotes )
T1025 130-131 -LRB- denotes [
T1026 131-133 CD denotes 25
T1027 133-134 -RRB- denotes ]
T1028 134-135 . denotes .
T1029 135-379 sentence denotes The DNA substrate contains a pair of mutated GFP genes (GFP encodes the easily detected green fluorescent protein), one of which contains a restriction site for I-SceI, a yeast intron encoded endonuclease with an 18 base pair recognition site.
T1030 136-139 DT denotes The
T1032 140-143 NN denotes DNA
T1031 144-153 NN denotes substrate
T1033 154-162 VBZ denotes contains
T1034 163-164 DT denotes a
T1035 165-169 NN denotes pair
T1036 170-172 IN denotes of
T1037 173-180 VBN denotes mutated
T1039 181-184 NN denotes GFP
T1038 185-190 NNS denotes genes
T1040 191-192 -LRB- denotes (
T1042 192-195 NN denotes GFP
T1041 196-203 VBZ denotes encodes
T1043 204-207 DT denotes the
T1045 208-214 RB denotes easily
T1046 215-223 VBN denotes detected
T1047 224-229 JJ denotes green
T1048 230-241 JJ denotes fluorescent
T1044 242-249 NN denotes protein
T1049 249-250 -RRB- denotes )
T1050 250-252 , denotes ,
T1051 252-255 CD denotes one
T1053 256-258 IN denotes of
T1054 259-264 WDT denotes which
T1052 265-273 VBZ denotes contains
T1055 274-275 DT denotes a
T1057 276-287 NN denotes restriction
T1056 288-292 NN denotes site
T1058 293-296 IN denotes for
T1059 297-298 NN denotes I
T1061 298-299 HYPH denotes -
T1060 299-303 NN denotes SceI
T1062 303-305 , denotes ,
T1063 305-306 DT denotes a
T1065 307-312 NN denotes yeast
T1066 313-319 NN denotes intron
T1067 320-327 VBN denotes encoded
T1064 328-340 NN denotes endonuclease
T1068 341-345 IN denotes with
T1069 346-348 DT denotes an
T1071 349-351 CD denotes 18
T1073 352-356 NN denotes base
T1072 357-361 NN denotes pair
T1074 362-373 NN denotes recognition
T1070 374-378 NN denotes site
T1075 378-379 . denotes .
T1076 379-502 sentence denotes Transient transfection of an I-SceI expression vector results in the production of a DSB in the first mutated copy of GFP.
T1077 380-389 JJ denotes Transient
T1078 390-402 NN denotes transfection
T1080 403-405 IN denotes of
T1081 406-408 DT denotes an
T1083 409-410 NN denotes I
T1085 410-411 HYPH denotes -
T1084 411-415 NN denotes SceI
T1086 416-426 NN denotes expression
T1082 427-433 NN denotes vector
T1079 434-441 VBZ denotes results
T1087 442-444 IN denotes in
T1088 445-448 DT denotes the
T1089 449-459 NN denotes production
T1090 460-462 IN denotes of
T1091 463-464 DT denotes a
T1092 465-468 NN denotes DSB
T1093 469-471 IN denotes in
T1094 472-475 DT denotes the
T1096 476-481 JJ denotes first
T1097 482-489 VBN denotes mutated
T1095 490-494 NN denotes copy
T1098 495-497 IN denotes of
T1099 498-501 NN denotes GFP
T1100 501-502 . denotes .
T1101 502-686 sentence denotes One or both DNA ends formed by the break invade(s) the homologous sequence in the second mutant GFP copy, resulting in repair of the DSB via a homology-mediated gene conversion event.
T1102 503-506 CD denotes One
T1104 507-509 CC denotes or
T1105 510-514 CC denotes both
T1107 515-518 NN denotes DNA
T1106 519-523 NNS denotes ends
T1108 524-530 VBN denotes formed
T1109 531-533 IN denotes by
T1110 534-537 DT denotes the
T1111 538-543 NN denotes break
T1103 544-550 VB denotes invade
T1112 550-551 -LRB- denotes (
T1113 551-552 VBZ denotes s
T1114 552-553 -RRB- denotes )
T1115 554-557 DT denotes the
T1117 558-568 JJ denotes homologous
T1116 569-577 NN denotes sequence
T1118 578-580 IN denotes in
T1119 581-584 DT denotes the
T1121 585-591 JJ denotes second
T1122 592-598 JJ denotes mutant
T1123 599-602 NN denotes GFP
T1120 603-607 NN denotes copy
T1124 607-609 , denotes ,
T1125 609-618 VBG denotes resulting
T1126 619-621 IN denotes in
T1127 622-628 NN denotes repair
T1128 629-631 IN denotes of
T1129 632-635 DT denotes the
T1130 636-639 NN denotes DSB
T1131 640-643 IN denotes via
T1132 644-645 DT denotes a
T1134 646-654 NN denotes homology
T1136 654-655 HYPH denotes -
T1135 655-663 VBN denotes mediated
T1137 664-668 NN denotes gene
T1138 669-679 NN denotes conversion
T1133 680-685 NN denotes event
T1139 685-686 . denotes .
T1140 686-823 sentence denotes The configuration of the GFP construct is such that homology-mediated repair often results in the formation of a functional copy of GFP.
T1141 687-690 DT denotes The
T1142 691-704 NN denotes configuration
T1144 705-707 IN denotes of
T1145 708-711 DT denotes the
T1147 712-715 NN denotes GFP
T1146 716-725 NN denotes construct
T1143 726-728 VBZ denotes is
T1148 729-733 JJ denotes such
T1149 734-738 IN denotes that
T1151 739-747 NN denotes homology
T1153 747-748 HYPH denotes -
T1152 748-756 VBN denotes mediated
T1154 757-763 NN denotes repair
T1155 764-769 RB denotes often
T1150 770-777 VBZ denotes results
T1156 778-780 IN denotes in
T1157 781-784 DT denotes the
T1158 785-794 NN denotes formation
T1159 795-797 IN denotes of
T1160 798-799 DT denotes a
T1162 800-810 JJ denotes functional
T1161 811-815 NN denotes copy
T1163 816-818 IN denotes of
T1164 819-822 NN denotes GFP
T1165 822-823 . denotes .
T1166 823-937 sentence denotes Such events can be detected by fluorescence-activated cell sorting analysis by virtue of their expression of GFP.
T1167 824-828 JJ denotes Such
T1168 829-835 NNS denotes events
T1170 836-839 MD denotes can
T1171 840-842 VB denotes be
T1169 843-851 VBN denotes detected
T1172 852-854 IN denotes by
T1173 855-867 NN denotes fluorescence
T1175 867-868 HYPH denotes -
T1174 868-877 VBN denotes activated
T1177 878-882 NN denotes cell
T1176 883-890 NN denotes sorting
T1178 891-899 NN denotes analysis
T1179 900-902 IN denotes by
T1180 903-909 NN denotes virtue
T1181 910-912 IN denotes of
T1182 913-918 PRP$ denotes their
T1183 919-929 NN denotes expression
T1184 930-932 IN denotes of
T1185 933-936 NN denotes GFP
T1186 936-937 . denotes .
R564 T1002 T1003 nsubj Pierce,designed
R565 T1004 T1005 advmod et,al
R566 T1005 T1002 advmod al,Pierce
R567 T1006 T1003 aux have,designed
R568 T1007 T1008 det a,set
R569 T1008 T1003 dobj set,designed
R570 T1009 T1008 prep of,set
R571 T1010 T1011 compound recombination,substrates
R572 T1011 T1009 pobj substrates,of
R573 T1012 T1003 prep for,designed
R574 T1013 T1012 pcomp measuring,for
R575 T1014 T1015 det the,level
R576 T1015 T1013 dobj level,measuring
R577 T1016 T1015 prep of,level
R578 T1017 T1018 amod homologous,recombination
R579 T1018 T1016 pobj recombination,of
R580 T1019 T1020 advmod in,vivo
R581 T1020 T1013 advmod vivo,measuring
R582 T1021 T1022 punct (,Fig.
R583 T1022 T1003 parataxis Fig.,designed
R584 T1023 T1022 nummod 1,Fig.
R585 T1024 T1022 punct ),Fig.
R586 T1025 T1026 punct [,25
R587 T1026 T1003 parataxis 25,designed
R588 T1027 T1026 punct ],25
R589 T1028 T1003 punct .,designed
R590 T1030 T1031 det The,substrate
R591 T1031 T1033 nsubj substrate,contains
R592 T1032 T1031 compound DNA,substrate
R593 T1034 T1035 det a,pair
R594 T1035 T1033 dobj pair,contains
R595 T1036 T1035 prep of,pair
R596 T1037 T1038 amod mutated,genes
R597 T1038 T1036 pobj genes,of
R598 T1039 T1038 compound GFP,genes
R599 T1040 T1041 punct (,encodes
R600 T1041 T1035 parataxis encodes,pair
R601 T1042 T1041 nsubj GFP,encodes
R602 T1043 T1044 det the,protein
R603 T1044 T1041 dobj protein,encodes
R604 T1045 T1046 advmod easily,detected
R605 T1046 T1044 amod detected,protein
R606 T1047 T1044 amod green,protein
R607 T1048 T1044 amod fluorescent,protein
R608 T1049 T1041 punct ),encodes
R609 T1050 T1035 punct ", ",pair
R610 T1051 T1052 dep one,contains
R611 T1052 T1035 relcl contains,pair
R612 T1053 T1051 prep of,one
R613 T1054 T1053 pobj which,of
R614 T1055 T1056 det a,site
R615 T1056 T1052 dobj site,contains
R616 T1057 T1056 compound restriction,site
R617 T1058 T1056 prep for,site
R618 T1059 T1060 compound I,SceI
R619 T1060 T1058 pobj SceI,for
R620 T1061 T1060 punct -,SceI
R621 T1062 T1060 punct ", ",SceI
R622 T1063 T1064 det a,endonuclease
R623 T1064 T1060 appos endonuclease,SceI
R624 T1065 T1066 compound yeast,intron
R625 T1066 T1067 npadvmod intron,encoded
R626 T1067 T1064 amod encoded,endonuclease
R627 T1068 T1064 prep with,endonuclease
R628 T1069 T1070 det an,site
R629 T1070 T1068 pobj site,with
R630 T1071 T1072 nummod 18,pair
R631 T1072 T1070 compound pair,site
R632 T1073 T1072 compound base,pair
R633 T1074 T1070 compound recognition,site
R634 T1075 T1033 punct .,contains
R635 T1077 T1078 amod Transient,transfection
R636 T1078 T1079 nsubj transfection,results
R637 T1080 T1078 prep of,transfection
R638 T1081 T1082 det an,vector
R639 T1082 T1080 pobj vector,of
R640 T1083 T1084 compound I,SceI
R641 T1084 T1082 compound SceI,vector
R642 T1085 T1084 punct -,SceI
R643 T1086 T1082 compound expression,vector
R644 T1087 T1079 prep in,results
R645 T1088 T1089 det the,production
R646 T1089 T1087 pobj production,in
R647 T1090 T1089 prep of,production
R648 T1091 T1092 det a,DSB
R649 T1092 T1090 pobj DSB,of
R650 T1093 T1089 prep in,production
R651 T1094 T1095 det the,copy
R652 T1095 T1093 pobj copy,in
R653 T1096 T1095 amod first,copy
R654 T1097 T1095 amod mutated,copy
R655 T1098 T1095 prep of,copy
R656 T1099 T1098 pobj GFP,of
R657 T1100 T1079 punct .,results
R658 T1102 T1103 nsubj One,invade
R659 T1104 T1102 cc or,One
R660 T1105 T1106 nmod both,ends
R661 T1106 T1102 conj ends,One
R662 T1107 T1106 compound DNA,ends
R663 T1108 T1102 acl formed,One
R664 T1109 T1108 agent by,formed
R665 T1110 T1111 det the,break
R666 T1111 T1109 pobj break,by
R667 T1112 T1103 punct (,invade
R668 T1113 T1103 dep s,invade
R669 T1114 T1103 punct ),invade
R670 T1115 T1116 det the,sequence
R671 T1116 T1103 dobj sequence,invade
R672 T1117 T1116 amod homologous,sequence
R673 T1118 T1116 prep in,sequence
R674 T1119 T1120 det the,copy
R675 T1120 T1118 pobj copy,in
R676 T1121 T1120 amod second,copy
R677 T1122 T1123 amod mutant,GFP
R678 T1123 T1120 compound GFP,copy
R679 T1124 T1103 punct ", ",invade
R680 T1125 T1103 advcl resulting,invade
R681 T1126 T1125 prep in,resulting
R682 T1127 T1126 pobj repair,in
R683 T1128 T1127 prep of,repair
R684 T1129 T1130 det the,DSB
R685 T1130 T1128 pobj DSB,of
R686 T1131 T1127 prep via,repair
R687 T1132 T1133 det a,event
R688 T1133 T1131 pobj event,via
R689 T1134 T1135 npadvmod homology,mediated
R690 T1135 T1133 amod mediated,event
R691 T1136 T1135 punct -,mediated
R692 T1137 T1138 compound gene,conversion
R693 T1138 T1133 compound conversion,event
R694 T1139 T1103 punct .,invade
R695 T1141 T1142 det The,configuration
R696 T1142 T1143 nsubj configuration,is
R697 T1144 T1142 prep of,configuration
R698 T1145 T1146 det the,construct
R699 T1146 T1144 pobj construct,of
R700 T1147 T1146 compound GFP,construct
R701 T1148 T1143 acomp such,is
R702 T1149 T1150 mark that,results
R703 T1150 T1148 ccomp results,such
R704 T1151 T1152 npadvmod homology,mediated
R705 T1152 T1154 amod mediated,repair
R706 T1153 T1152 punct -,mediated
R707 T1154 T1150 nsubj repair,results
R708 T1155 T1150 advmod often,results
R709 T1156 T1150 prep in,results
R710 T1157 T1158 det the,formation
R711 T1158 T1156 pobj formation,in
R712 T1159 T1158 prep of,formation
R713 T1160 T1161 det a,copy
R714 T1161 T1159 pobj copy,of
R715 T1162 T1161 amod functional,copy
R716 T1163 T1161 prep of,copy
R717 T1164 T1163 pobj GFP,of
R718 T1165 T1143 punct .,is
R719 T1167 T1168 amod Such,events
R720 T1168 T1169 nsubjpass events,detected
R721 T1170 T1169 aux can,detected
R722 T1171 T1169 auxpass be,detected
R723 T1172 T1169 prep by,detected
R724 T1173 T1174 npadvmod fluorescence,activated
R725 T1174 T1176 amod activated,sorting
R726 T1175 T1174 punct -,activated
R727 T1176 T1178 compound sorting,analysis
R728 T1177 T1176 compound cell,sorting
R729 T1178 T1172 pobj analysis,by
R730 T1179 T1169 prep by,detected
R731 T1180 T1179 pobj virtue,by
R732 T1181 T1180 prep of,virtue
R733 T1182 T1183 poss their,expression
R734 T1183 T1181 pobj expression,of
R735 T1184 T1183 prep of,expression
R736 T1185 T1184 pobj GFP,of
R737 T1186 T1169 punct .,detected