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PMC:1382200 / 9525-15784 JSONTXT 4 Projects

Annnotations TAB TSV DIC JSON TextAE

Id Subject Object Predicate Lexical cue
T6635 0-10 NN denotes Generation
T6636 11-13 IN denotes of
T6637 14-22 JJ denotes chimeric
T6638 23-27 NNS denotes mice
T6639 28-31 CC denotes and
T6640 32-40 NN denotes germline
T6641 41-53 NN denotes transmission
T6642 54-56 IN denotes of
T6643 57-60 DT denotes the
T6645 61-67 NN denotes mutant
T6646 68-73 NN denotes Capn2
T6644 74-80 NN denotes allele
T6647 80-188 sentence denotes Eight chimeric male mice were produced in morula aggregation experiments using the Capn2+/- ES27 cell line.
T6648 81-86 CD denotes Eight
T6650 87-95 JJ denotes chimeric
T6651 96-100 JJ denotes male
T6649 101-105 NNS denotes mice
T6653 106-110 VBD denotes were
T6652 111-119 VBN denotes produced
T6654 120-122 IN denotes in
T6655 123-129 NN denotes morula
T6656 130-141 NN denotes aggregation
T6657 142-153 NNS denotes experiments
T6658 154-159 VBG denotes using
T6659 160-163 DT denotes the
T6661 164-169 NN denotes Capn2
T6662 169-170 SYM denotes +
T6663 170-171 HYPH denotes /
T6664 171-172 SYM denotes -
T6665 173-177 NN denotes ES27
T6666 178-182 NN denotes cell
T6660 183-187 NN denotes line
T6667 187-188 . denotes .
T6668 188-291 sentence denotes Two of these males transmitted the Capn2+/- ES27 genotype through the germline into the F1 generation.
T6669 189-192 CD denotes Two
T6671 193-195 IN denotes of
T6672 196-201 DT denotes these
T6673 202-207 NNS denotes males
T6670 208-219 VBD denotes transmitted
T6674 220-223 DT denotes the
T6676 224-229 NN denotes Capn2
T6677 229-230 SYM denotes +
T6678 230-231 HYPH denotes /
T6679 231-232 SYM denotes -
T6680 233-237 NN denotes ES27
T6675 238-246 NN denotes genotype
T6681 247-254 IN denotes through
T6682 255-258 DT denotes the
T6683 259-267 NN denotes germline
T6684 268-272 IN denotes into
T6685 273-276 DT denotes the
T6687 277-279 NN denotes F1
T6686 280-290 NN denotes generation
T6688 290-291 . denotes .
T6689 291-408 sentence denotes Heterozygous Capn2+/- animals appeared normal, with no obvious defects in gross anatomy, reproduction, or life span.
T6690 292-304 JJ denotes Heterozygous
T6692 305-310 NN denotes Capn2
T6693 310-311 SYM denotes +
T6694 311-312 HYPH denotes /
T6695 312-313 SYM denotes -
T6691 314-321 NNS denotes animals
T6696 322-330 VBD denotes appeared
T6697 331-337 JJ denotes normal
T6698 337-339 , denotes ,
T6699 339-343 IN denotes with
T6700 344-346 DT denotes no
T6702 347-354 JJ denotes obvious
T6701 355-362 NNS denotes defects
T6703 363-365 IN denotes in
T6704 366-371 JJ denotes gross
T6705 372-379 NN denotes anatomy
T6706 379-381 , denotes ,
T6707 381-393 NN denotes reproduction
T6708 393-395 , denotes ,
T6709 395-397 CC denotes or
T6710 398-402 NN denotes life
T6711 403-407 NN denotes span
T6712 407-408 . denotes .
T6713 408-506 sentence denotes Out of 199 weanlings from heterozygous intercrosses, no Capn2-/- progeny were detected (Table 2).
T6714 409-412 IN denotes Out
T6716 413-415 IN denotes of
T6717 416-419 CD denotes 199
T6718 420-429 NNS denotes weanlings
T6719 430-434 IN denotes from
T6720 435-447 JJ denotes heterozygous
T6721 448-460 NNS denotes intercrosses
T6722 460-462 , denotes ,
T6723 462-464 DT denotes no
T6725 465-470 NN denotes Capn2
T6726 470-471 SYM denotes -
T6727 471-472 HYPH denotes /
T6728 472-473 SYM denotes -
T6724 474-481 NN denotes progeny
T6729 482-486 VBD denotes were
T6715 487-495 VBN denotes detected
T6730 496-497 -LRB- denotes (
T6731 497-502 NN denotes Table
T6732 503-504 CD denotes 2
T6733 504-505 -RRB- denotes )
T6734 505-506 . denotes .
T6735 506-598 sentence denotes We did not observe high rates of perinatal death, and no Capn2-/- stillborns were observed.
T6736 507-509 PRP denotes We
T6738 510-513 VBD denotes did
T6739 514-517 RB denotes not
T6737 518-525 VB denotes observe
T6740 526-530 JJ denotes high
T6741 531-536 NNS denotes rates
T6742 537-539 IN denotes of
T6743 540-549 JJ denotes perinatal
T6744 550-555 NN denotes death
T6745 555-557 , denotes ,
T6746 557-560 CC denotes and
T6747 561-563 DT denotes no
T6749 564-569 NN denotes Capn2
T6750 569-570 SYM denotes -
T6751 570-571 HYPH denotes /
T6752 571-572 SYM denotes -
T6748 573-583 NNS denotes stillborns
T6754 584-588 VBD denotes were
T6753 589-597 VBN denotes observed
T6755 597-598 . denotes .
T6756 598-688 sentence denotes This suggested that Capn2-/- animals perished at some stage during embryonic development.
T6757 599-603 DT denotes This
T6758 604-613 VBD denotes suggested
T6759 614-618 IN denotes that
T6761 619-624 NN denotes Capn2
T6763 624-625 SYM denotes -
T6764 625-626 HYPH denotes /
T6765 626-627 SYM denotes -
T6762 628-635 NNS denotes animals
T6760 636-644 VBN denotes perished
T6766 645-647 IN denotes at
T6767 648-652 DT denotes some
T6768 653-658 NN denotes stage
T6769 659-665 IN denotes during
T6770 666-675 JJ denotes embryonic
T6771 676-687 NN denotes development
T6772 687-688 . denotes .
T6773 688-855 sentence denotes In an attempt to determine if embryonic death occurred at a post-implantation stage, embryos were harvested for genotyping at different times between E10.5 and E18.5.
T6774 689-691 IN denotes In
T6776 692-694 DT denotes an
T6777 695-702 NN denotes attempt
T6778 703-705 TO denotes to
T6779 706-715 VB denotes determine
T6780 716-718 IN denotes if
T6782 719-728 JJ denotes embryonic
T6783 729-734 NN denotes death
T6781 735-743 VBD denotes occurred
T6784 744-746 IN denotes at
T6785 747-748 DT denotes a
T6787 749-766 JJ denotes post-implantation
T6786 767-772 NN denotes stage
T6788 772-774 , denotes ,
T6789 774-781 NNS denotes embryos
T6790 782-786 VBD denotes were
T6775 787-796 VBN denotes harvested
T6791 797-800 IN denotes for
T6792 801-811 NN denotes genotyping
T6793 812-814 IN denotes at
T6794 815-824 JJ denotes different
T6795 825-830 NNS denotes times
T6796 831-838 IN denotes between
T6797 839-844 NN denotes E10.5
T6798 845-848 CC denotes and
T6799 849-854 NN denotes E18.5
T6800 854-855 . denotes .
T6801 855-948 sentence denotes No Capn2-/- embryos were observed and no signs of embryo resorption were detected (Table 2).
T6802 856-858 DT denotes No
T6804 859-864 NN denotes Capn2
T6805 864-865 SYM denotes -
T6806 865-866 HYPH denotes /
T6807 866-867 SYM denotes -
T6803 868-875 NNS denotes embryos
T6809 876-880 VBD denotes were
T6808 881-889 VBN denotes observed
T6810 890-893 CC denotes and
T6811 894-896 DT denotes no
T6812 897-902 NNS denotes signs
T6814 903-905 IN denotes of
T6815 906-912 NN denotes embryo
T6816 913-923 NN denotes resorption
T6817 924-928 VBD denotes were
T6813 929-937 VBN denotes detected
T6818 938-939 -LRB- denotes (
T6819 939-944 NN denotes Table
T6820 945-946 CD denotes 2
T6821 946-947 -RRB- denotes )
T6822 947-948 . denotes .
T6823 948-1027 sentence denotes This indicated that the Capn2-/- embryos might be dying prior to implantation.
T6824 949-953 DT denotes This
T6825 954-963 VBD denotes indicated
T6826 964-968 IN denotes that
T6828 969-972 DT denotes the
T6830 973-978 NN denotes Capn2
T6831 978-979 SYM denotes -
T6832 979-980 HYPH denotes /
T6833 980-981 SYM denotes -
T6829 982-989 NNS denotes embryos
T6834 990-995 MD denotes might
T6835 996-998 VB denotes be
T6827 999-1004 VBG denotes dying
T6836 1005-1010 JJ denotes prior
T6837 1011-1013 IN denotes to
T6838 1014-1026 NN denotes implantation
T6839 1026-1027 . denotes .
T6840 1027-1168 sentence denotes Embryos were then flushed from the oviducts of pregnant females at E2.5 or E3.5, and genotyped by means of a nested PCR strategy (Figure 4).
T6841 1028-1035 NNS denotes Embryos
T6843 1036-1040 VBD denotes were
T6844 1041-1045 RB denotes then
T6842 1046-1053 VBN denotes flushed
T6845 1054-1058 IN denotes from
T6846 1059-1062 DT denotes the
T6847 1063-1071 NNS denotes oviducts
T6848 1072-1074 IN denotes of
T6849 1075-1083 JJ denotes pregnant
T6850 1084-1091 NNS denotes females
T6851 1092-1094 IN denotes at
T6852 1095-1099 NN denotes E2.5
T6853 1100-1102 CC denotes or
T6854 1103-1107 NN denotes E3.5
T6855 1107-1109 , denotes ,
T6856 1109-1112 CC denotes and
T6857 1113-1122 VBN denotes genotyped
T6858 1123-1125 IN denotes by
T6859 1126-1131 NNS denotes means
T6860 1132-1134 IN denotes of
T6861 1135-1136 DT denotes a
T6863 1137-1143 VBN denotes nested
T6864 1144-1147 NN denotes PCR
T6862 1148-1156 NN denotes strategy
T6865 1157-1158 -LRB- denotes (
T6866 1158-1164 NN denotes Figure
T6867 1165-1166 CD denotes 4
T6868 1166-1167 -RRB- denotes )
T6869 1167-1168 . denotes .
T6870 1168-1262 sentence denotes Two of 90 successfully genotyped pre-implantation embryos were Capn2-/-, (Table 2; Figure 5).
T6871 1169-1172 CD denotes Two
T6873 1173-1175 IN denotes of
T6874 1176-1178 CD denotes 90
T6876 1179-1191 RB denotes successfully
T6877 1192-1201 VBN denotes genotyped
T6878 1202-1218 JJ denotes pre-implantation
T6875 1219-1226 NNS denotes embryos
T6872 1227-1231 VBD denotes were
T6879 1232-1237 NN denotes Capn2
T6880 1237-1238 SYM denotes -
T6881 1238-1239 HYPH denotes /
T6882 1239-1240 SYM denotes -
T6883 1240-1242 , denotes ,
T6884 1242-1243 -LRB- denotes (
T6886 1243-1248 NN denotes Table
T6887 1249-1250 CD denotes 2
T6888 1250-1251 : denotes ;
T6885 1252-1258 NN denotes Figure
T6889 1259-1260 CD denotes 5
T6890 1260-1261 -RRB- denotes )
T6891 1261-1262 . denotes .
T6892 1262-1382 sentence denotes Both of these Capn2-/- embryos were isolated at the 8-cell stage and did not display any obvious morphological defects.
T6893 1263-1267 DT denotes Both
T6895 1268-1270 IN denotes of
T6896 1271-1276 DT denotes these
T6898 1277-1282 NN denotes Capn2
T6899 1282-1283 SYM denotes -
T6900 1283-1284 HYPH denotes /
T6901 1284-1285 SYM denotes -
T6897 1286-1293 NNS denotes embryos
T6902 1294-1298 VBD denotes were
T6894 1299-1307 VBN denotes isolated
T6903 1308-1310 IN denotes at
T6904 1311-1314 DT denotes the
T6906 1315-1316 CD denotes 8
T6908 1316-1317 HYPH denotes -
T6907 1317-1321 NN denotes cell
T6905 1322-1327 NN denotes stage
T6909 1328-1331 CC denotes and
T6910 1332-1335 VBD denotes did
T6912 1336-1339 RB denotes not
T6911 1340-1347 VB denotes display
T6913 1348-1351 DT denotes any
T6915 1352-1359 JJ denotes obvious
T6916 1360-1373 JJ denotes morphological
T6914 1374-1381 NNS denotes defects
T6917 1381-1382 . denotes .
T6918 1382-1461 sentence denotes None of the 46 successfully genotyped blastocyst-staged embryos were Capn2-/-.
T6919 1383-1387 NN denotes None
T6921 1388-1390 IN denotes of
T6922 1391-1394 DT denotes the
T6924 1395-1397 CD denotes 46
T6925 1398-1410 RB denotes successfully
T6926 1411-1420 VBN denotes genotyped
T6927 1421-1431 NN denotes blastocyst
T6929 1431-1432 HYPH denotes -
T6928 1432-1438 VBN denotes staged
T6923 1439-1446 NNS denotes embryos
T6920 1447-1451 VBD denotes were
T6930 1452-1457 NN denotes Capn2
T6931 1457-1458 SYM denotes -
T6932 1458-1459 HYPH denotes /
T6933 1459-1460 SYM denotes -
T6934 1460-1461 . denotes .
T6935 1461-1633 sentence denotes The scarcity of Capn2-deficient embryos surviving to the 8-cell stage suggested that the loss of m-calpain activity must fatally compromise the viability of early embryos.
T6936 1462-1465 DT denotes The
T6937 1466-1474 NN denotes scarcity
T6939 1475-1477 IN denotes of
T6940 1478-1483 NN denotes Capn2
T6942 1483-1484 HYPH denotes -
T6941 1484-1493 JJ denotes deficient
T6943 1494-1501 NNS denotes embryos
T6944 1502-1511 VBG denotes surviving
T6945 1512-1514 IN denotes to
T6946 1515-1518 DT denotes the
T6948 1519-1520 CD denotes 8
T6950 1520-1521 HYPH denotes -
T6949 1521-1525 NN denotes cell
T6947 1526-1531 NN denotes stage
T6938 1532-1541 VBD denotes suggested
T6951 1542-1546 IN denotes that
T6953 1547-1550 DT denotes the
T6954 1551-1555 NN denotes loss
T6955 1556-1558 IN denotes of
T6956 1559-1560 NN denotes m
T6958 1560-1561 HYPH denotes -
T6957 1561-1568 NN denotes calpain
T6959 1569-1577 NN denotes activity
T6960 1578-1582 MD denotes must
T6961 1583-1590 RB denotes fatally
T6952 1591-1601 VB denotes compromise
T6962 1602-1605 DT denotes the
T6963 1606-1615 NN denotes viability
T6964 1616-1618 IN denotes of
T6965 1619-1624 JJ denotes early
T6966 1625-1632 NNS denotes embryos
T6967 1632-1633 . denotes .
T6968 1633-1818 sentence denotes Furthermore, it is possible that persistence of some maternally derived mRNA transcript or protein might have allowed a small number of Capn2-/- embryos to survive to the morula-stage.
T6969 1634-1645 RB denotes Furthermore
T6971 1645-1647 , denotes ,
T6972 1647-1649 PRP denotes it
T6970 1650-1652 VBZ denotes is
T6973 1653-1661 JJ denotes possible
T6974 1662-1666 IN denotes that
T6976 1667-1678 NN denotes persistence
T6977 1679-1681 IN denotes of
T6978 1682-1686 DT denotes some
T6980 1687-1697 RB denotes maternally
T6981 1698-1705 VBN denotes derived
T6982 1706-1710 NN denotes mRNA
T6979 1711-1721 NN denotes transcript
T6983 1722-1724 CC denotes or
T6984 1725-1732 NN denotes protein
T6985 1733-1738 MD denotes might
T6986 1739-1743 VB denotes have
T6975 1744-1751 VBN denotes allowed
T6987 1752-1753 DT denotes a
T6989 1754-1759 JJ denotes small
T6988 1760-1766 NN denotes number
T6991 1767-1769 IN denotes of
T6992 1770-1775 NN denotes Capn2
T6994 1775-1776 SYM denotes -
T6995 1776-1777 HYPH denotes /
T6996 1777-1778 SYM denotes -
T6993 1779-1786 NNS denotes embryos
T6997 1787-1789 TO denotes to
T6990 1790-1797 VB denotes survive
T6998 1798-1800 IN denotes to
T6999 1801-1804 DT denotes the
T7001 1805-1811 NN denotes morula
T7002 1811-1812 HYPH denotes -
T7000 1812-1817 NN denotes stage
T7003 1817-1818 . denotes .
T7004 1818-4790 sentence denotes Table 2 Genotype distribution of offspring derived from Capn2 transgenic mice. Cross (N) Age Genotype +/+ +/- -/- ND* (+/-)♀ × (+/-)♂ 199 3 weeks 23 176 0 0 (+/+)♀ × (+/-)♂ 122 3 weeks 50 72 0 0 (+/-)♀ × (+/+)♂ 103 3 weeks 28 75 0 0 (+/-)♀ × (+/-)♂ 9 E18.5 0 7 0 2 (+/-)♀ × (+/-)♂ 5 E17.5 0 5 0 0 (+/-)♀ × (+/-)♂ 9 E14.5 2 7 0 0 (+/-)♀ × (+/-)♂ 10 E11.5 3 7 0 0 (+/-)♀ × (+/-)♂ 7 E10.5 2 5 0 0 (+/-)♀ × (+/-)♂ 46 E3.5 7 39 0 0 (+/-)♀ × (+/-)♂ 48 E2.5 8 34 2 4 * ND, not determined Figure 4 Nested PCR strategy for genotyping of pre-implantation embryos. Due to the scarcity of extractable genetic material, a nested PCR strategy was developed in order to genotype pre-implantation embryos. Separate reactions were used to amplify a 429 bp fragment from the wild-type allele and a 389 bp segment from the mutant allele, both spanning the 3' end of the short (upstream) arm of the targeting vector. In both reactions, a 213 bp sequence located within the short arm was co-amplified with the 'diagnostic' products as an internal control. The same sense primers were used to amplify 'diagnostic' sequences in both reactions, whereas the antisense primers were allele-specific. The primers, represented by triangles, are depicted in two (nested) sets for each of the three reactions. Exons are represented by open, vertical rectangles, the PGK-Neo cassette by an open, horizontal rectangle, while thin vertical lines denote the boundaries of the short arm and the 5' end of the long (downstream) arm. A grey, horizontal rectangle delineates the segment of the wild-type allele that is replaced by the PGK-Neo cassette in the mutant allele. Figure 5 Genotyping of pre-implantation embryos. A nested PCR strategy was used to genotype embryos prior to implantation as described in Figure 4. Capn2+/- mice were mated and the date of fertilization established by the appearance of vaginal plugs. Blastocyst (E3.5) or 8-cell embryos (E2.5) were flushed from the oviducts and then digested with proteinase K. In separate reactions segments found exclusively in either the wild-type or mutant alleles were co-amplified with an internal control sequence, located in the short (upstream) arm of the targeting vector, which is found in both alleles. The final products were 429 bp for the wild-type allele, 389 bp for the mutant allele, and 213 bp for the internal control. (A) A representative example of the genotyping of blastocyst stage embryos. Embryos #1, 2, 4, 5, and 6 were Capn2+/- whereas embryos #3 and #7 were Capn2+/+, denoted by the absence of the 389 bp mutant signal. (B) An example of the genotyping of 8-cell embryos is shown. Embryos #1, 2, 3, 5, and 6 were Capn2+/- while embryo #4 was Capn2-/-, marked by the absence of the 429 bp wild-type signal. (M) denotes the molecular weight marker. The genotyping results for weanlings, post-implantation, and pre-implantation embryos are shown in Table 2.
T7005 1819-4686 DT denotes Table 2 Genotype distribution of offspring derived from Capn2 transgenic mice. Cross (N) Age Genotype +/+ +/- -/- ND* (+/-)♀ × (+/-)♂ 199 3 weeks 23 176 0 0 (+/+)♀ × (+/-)♂ 122 3 weeks 50 72 0 0 (+/-)♀ × (+/+)♂ 103 3 weeks 28 75 0 0 (+/-)♀ × (+/-)♂ 9 E18.5 0 7 0 2 (+/-)♀ × (+/-)♂ 5 E17.5 0 5 0 0 (+/-)♀ × (+/-)♂ 9 E14.5 2 7 0 0 (+/-)♀ × (+/-)♂ 10 E11.5 3 7 0 0 (+/-)♀ × (+/-)♂ 7 E10.5 2 5 0 0 (+/-)♀ × (+/-)♂ 46 E3.5 7 39 0 0 (+/-)♀ × (+/-)♂ 48 E2.5 8 34 2 4 * ND, not determined Figure 4 Nested PCR strategy for genotyping of pre-implantation embryos. Due to the scarcity of extractable genetic material, a nested PCR strategy was developed in order to genotype pre-implantation embryos. Separate reactions were used to amplify a 429 bp fragment from the wild-type allele and a 389 bp segment from the mutant allele, both spanning the 3' end of the short (upstream) arm of the targeting vector. In both reactions, a 213 bp sequence located within the short arm was co-amplified with the 'diagnostic' products as an internal control. The same sense primers were used to amplify 'diagnostic' sequences in both reactions, whereas the antisense primers were allele-specific. The primers, represented by triangles, are depicted in two (nested) sets for each of the three reactions. Exons are represented by open, vertical rectangles, the PGK-Neo cassette by an open, horizontal rectangle, while thin vertical lines denote the boundaries of the short arm and the 5' end of the long (downstream) arm. A grey, horizontal rectangle delineates the segment of the wild-type allele that is replaced by the PGK-Neo cassette in the mutant allele. Figure 5 Genotyping of pre-implantation embryos. A nested PCR strategy was used to genotype embryos prior to implantation as described in Figure 4. Capn2+/- mice were mated and the date of fertilization established by the appearance of vaginal plugs. Blastocyst (E3.5) or 8-cell embryos (E2.5) were flushed from the oviducts and then digested with proteinase K. In separate reactions segments found exclusively in either the wild-type or mutant alleles were co-amplified with an internal control sequence, located in the short (upstream) arm of the targeting vector, which is found in both alleles. The final products were 429 bp for the wild-type allele, 389 bp for the mutant allele, and 213 bp for the internal control. (A) A representative example of the genotyping of blastocyst stage embryos. Embryos #1, 2, 4, 5, and 6 were Capn2+/- whereas embryos #3 and #7 were Capn2+/+, denoted by the absence of the 389 bp mutant signal. (B) An example of the genotyping of 8-cell embryos is shown. Embryos #1, 2, 3, 5, and 6 were Capn2+/- while embryo #4 was Capn2-/-, marked by the absence of the 429 bp wild-type signal. (M) denotes the molecular weight marker. The
T22936 1828-1836 NN denotes Genotype
T22937 1837-1849 NN denotes distribution
T22938 1850-1852 IN denotes of
T22939 1853-1862 NN denotes offspring
T22940 1863-1870 VBN denotes derived
T22941 1871-1875 IN denotes from
T22942 1876-1881 NN denotes Capn2
T22944 1882-1892 JJ denotes transgenic
T22943 1893-1897 NNS denotes mice
T22945 1897-1898 . denotes .
T21857 2377-2383 VBN denotes Nested
T21859 2384-2387 NN denotes PCR
T21858 2388-2396 NN denotes strategy
T21860 2397-2400 IN denotes for
T21861 2401-2411 NN denotes genotyping
T21862 2412-2414 IN denotes of
T21863 2415-2431 JJ denotes pre-implantation
T21864 2432-2439 NNS denotes embryos
T21865 2439-2440 . denotes .
T21866 2440-2576 sentence denotes Due to the scarcity of extractable genetic material, a nested PCR strategy was developed in order to genotype pre-implantation embryos.
T21867 2441-2444 IN denotes Due
T21869 2445-2447 IN denotes to
T21870 2448-2451 DT denotes the
T21871 2452-2460 NN denotes scarcity
T21872 2461-2463 IN denotes of
T21873 2464-2475 JJ denotes extractable
T21875 2476-2483 JJ denotes genetic
T21874 2484-2492 NN denotes material
T21876 2492-2494 , denotes ,
T21877 2494-2495 DT denotes a
T21879 2496-2502 VBN denotes nested
T21880 2503-2506 NN denotes PCR
T21878 2507-2515 NN denotes strategy
T21881 2516-2519 VBD denotes was
T21868 2520-2529 VBN denotes developed
T21882 2530-2532 IN denotes in
T21883 2533-2538 NN denotes order
T21884 2539-2541 TO denotes to
T21885 2542-2550 VB denotes genotype
T21886 2551-2567 JJ denotes pre-implantation
T21887 2568-2575 NNS denotes embryos
T21888 2575-2576 . denotes .
T21889 2576-2783 sentence denotes Separate reactions were used to amplify a 429 bp fragment from the wild-type allele and a 389 bp segment from the mutant allele, both spanning the 3' end of the short (upstream) arm of the targeting vector.
T21890 2577-2585 JJ denotes Separate
T21891 2586-2595 NNS denotes reactions
T21893 2596-2600 VBD denotes were
T21892 2601-2605 VBN denotes used
T21894 2606-2608 TO denotes to
T21895 2609-2616 VB denotes amplify
T21896 2617-2618 DT denotes a
T21898 2619-2622 CD denotes 429
T21899 2623-2625 NN denotes bp
T21897 2626-2634 NN denotes fragment
T21900 2635-2639 IN denotes from
T21901 2640-2643 DT denotes the
T21903 2644-2648 JJ denotes wild
T21905 2648-2649 HYPH denotes -
T21904 2649-2653 NN denotes type
T21902 2654-2660 NN denotes allele
T21906 2661-2664 CC denotes and
T21907 2665-2666 DT denotes a
T21909 2667-2670 CD denotes 389
T21910 2671-2673 NN denotes bp
T21908 2674-2681 NN denotes segment
T21911 2682-2686 IN denotes from
T21912 2687-2690 DT denotes the
T21914 2691-2697 NN denotes mutant
T21913 2698-2704 NN denotes allele
T21915 2704-2706 , denotes ,
T21916 2706-2710 DT denotes both
T21917 2711-2719 VBG denotes spanning
T21918 2720-2723 DT denotes the
T21920 2724-2725 CD denotes 3
T21921 2725-2726 SYM denotes '
T21919 2727-2730 NN denotes end
T21922 2731-2733 IN denotes of
T21923 2734-2737 DT denotes the
T21925 2738-2743 JJ denotes short
T21926 2744-2745 -LRB- denotes (
T21927 2745-2753 JJ denotes upstream
T21928 2753-2754 -RRB- denotes )
T21924 2755-2758 NN denotes arm
T21929 2759-2761 IN denotes of
T21930 2762-2765 DT denotes the
T21932 2766-2775 NN denotes targeting
T21931 2776-2782 NN denotes vector
T21933 2782-2783 . denotes .
T21934 2783-2921 sentence denotes In both reactions, a 213 bp sequence located within the short arm was co-amplified with the 'diagnostic' products as an internal control.
T21935 2784-2786 IN denotes In
T21937 2787-2791 DT denotes both
T21938 2792-2801 NNS denotes reactions
T21939 2801-2803 , denotes ,
T21940 2803-2804 DT denotes a
T21942 2805-2808 CD denotes 213
T21943 2809-2811 NN denotes bp
T21941 2812-2820 NN denotes sequence
T21944 2821-2828 VBN denotes located
T21945 2829-2835 IN denotes within
T21946 2836-2839 DT denotes the
T21948 2840-2845 JJ denotes short
T21947 2846-2849 NN denotes arm
T21949 2850-2853 VBD denotes was
T21936 2854-2866 VBN denotes co-amplified
T21950 2867-2871 IN denotes with
T21951 2872-2875 DT denotes the
T21953 2876-2877 `` denotes '
T21954 2877-2887 JJ denotes diagnostic
T21955 2887-2888 '' denotes '
T21952 2889-2897 NNS denotes products
T21956 2898-2900 IN denotes as
T21957 2901-2903 DT denotes an
T21959 2904-2912 JJ denotes internal
T21958 2913-2920 NN denotes control
T21960 2920-2921 . denotes .
T21961 2921-3059 sentence denotes The same sense primers were used to amplify 'diagnostic' sequences in both reactions, whereas the antisense primers were allele-specific.
T21962 2922-2925 DT denotes The
T21964 2926-2930 JJ denotes same
T21965 2931-2936 NN denotes sense
T21963 2937-2944 NNS denotes primers
T21967 2945-2949 VBD denotes were
T21966 2950-2954 VBN denotes used
T21968 2955-2957 TO denotes to
T21969 2958-2965 VB denotes amplify
T21970 2966-2967 `` denotes '
T21971 2967-2977 JJ denotes diagnostic
T21973 2977-2978 '' denotes '
T21972 2979-2988 NNS denotes sequences
T21974 2989-2991 IN denotes in
T21975 2992-2996 DT denotes both
T21976 2997-3006 NNS denotes reactions
T21977 3006-3008 , denotes ,
T21978 3008-3015 IN denotes whereas
T21980 3016-3019 DT denotes the
T21982 3020-3029 NN denotes antisense
T21981 3030-3037 NNS denotes primers
T21979 3038-3042 VBD denotes were
T21983 3043-3049 NN denotes allele
T21985 3049-3050 HYPH denotes -
T21984 3050-3058 JJ denotes specific
T21986 3058-3059 . denotes .
T21987 3059-3165 sentence denotes The primers, represented by triangles, are depicted in two (nested) sets for each of the three reactions.
T21988 3060-3063 DT denotes The
T21989 3064-3071 NNS denotes primers
T21991 3071-3073 , denotes ,
T21992 3073-3084 VBN denotes represented
T21993 3085-3087 IN denotes by
T21994 3088-3097 NNS denotes triangles
T21995 3097-3099 , denotes ,
T21996 3099-3102 VBP denotes are
T21990 3103-3111 VBN denotes depicted
T21997 3112-3114 IN denotes in
T21998 3115-3118 CD denotes two
T22000 3119-3120 -LRB- denotes (
T22001 3120-3126 VBN denotes nested
T22002 3126-3127 -RRB- denotes )
T21999 3128-3132 NNS denotes sets
T22003 3133-3136 IN denotes for
T22004 3137-3141 DT denotes each
T22005 3142-3144 IN denotes of
T22006 3145-3148 DT denotes the
T22008 3149-3154 CD denotes three
T22007 3155-3164 NNS denotes reactions
T22009 3164-3165 . denotes .
T22010 3165-3382 sentence denotes Exons are represented by open, vertical rectangles, the PGK-Neo cassette by an open, horizontal rectangle, while thin vertical lines denote the boundaries of the short arm and the 5' end of the long (downstream) arm.
T22011 3166-3171 NNS denotes Exons
T22013 3172-3175 VBP denotes are
T22012 3176-3187 VBN denotes represented
T22015 3188-3190 IN denotes by
T22016 3191-3195 JJ denotes open
T22018 3195-3197 , denotes ,
T22019 3197-3205 JJ denotes vertical
T22017 3206-3216 NNS denotes rectangles
T22020 3216-3218 , denotes ,
T22021 3218-3221 DT denotes the
T22022 3222-3225 NN denotes PGK
T22024 3225-3226 HYPH denotes -
T22023 3226-3229 NN denotes Neo
T22014 3230-3238 NN denotes cassette
T22025 3239-3241 IN denotes by
T22026 3242-3244 DT denotes an
T22028 3245-3249 JJ denotes open
T22029 3249-3251 , denotes ,
T22030 3251-3261 JJ denotes horizontal
T22027 3262-3271 NN denotes rectangle
T22031 3271-3273 , denotes ,
T22032 3273-3278 IN denotes while
T22034 3279-3283 JJ denotes thin
T22036 3284-3292 JJ denotes vertical
T22035 3293-3298 NNS denotes lines
T22033 3299-3305 VBP denotes denote
T22037 3306-3309 DT denotes the
T22038 3310-3320 NNS denotes boundaries
T22039 3321-3323 IN denotes of
T22040 3324-3327 DT denotes the
T22042 3328-3333 JJ denotes short
T22041 3334-3337 NN denotes arm
T22043 3338-3341 CC denotes and
T22044 3342-3345 DT denotes the
T22046 3346-3347 CD denotes 5
T22047 3347-3348 SYM denotes '
T22045 3349-3352 NN denotes end
T22048 3353-3355 IN denotes of
T22049 3356-3359 DT denotes the
T22051 3360-3364 JJ denotes long
T22052 3365-3366 -LRB- denotes (
T22053 3366-3376 JJ denotes downstream
T22054 3376-3377 -RRB- denotes )
T22050 3378-3381 NN denotes arm
T22055 3381-3382 . denotes .
T22056 3382-3521 sentence denotes A grey, horizontal rectangle delineates the segment of the wild-type allele that is replaced by the PGK-Neo cassette in the mutant allele.
T22057 3383-3384 DT denotes A
T22059 3385-3389 JJ denotes grey
T22060 3389-3391 , denotes ,
T22061 3391-3401 JJ denotes horizontal
T22058 3402-3411 NN denotes rectangle
T22062 3412-3422 VBZ denotes delineates
T22063 3423-3426 DT denotes the
T22064 3427-3434 NN denotes segment
T22065 3435-3437 IN denotes of
T22066 3438-3441 DT denotes the
T22068 3442-3446 JJ denotes wild
T22070 3446-3447 HYPH denotes -
T22069 3447-3451 NN denotes type
T22067 3452-3458 NN denotes allele
T22071 3459-3463 WDT denotes that
T22073 3464-3466 VBZ denotes is
T22072 3467-3475 VBN denotes replaced
T22074 3476-3478 IN denotes by
T22075 3479-3482 DT denotes the
T22077 3483-3486 NN denotes PGK
T22079 3486-3487 HYPH denotes -
T22078 3487-3490 NN denotes Neo
T22076 3491-3499 NN denotes cassette
T22080 3500-3502 IN denotes in
T22081 3503-3506 DT denotes the
T22083 3507-3513 NN denotes mutant
T22082 3514-3520 NN denotes allele
T22084 3520-3521 . denotes .
T22640 3532-3542 NN denotes Genotyping
T22641 3543-3545 IN denotes of
T22642 3546-3562 JJ denotes pre-implantation
T22643 3563-3570 NNS denotes embryos
T22644 3570-3571 . denotes .
T22645 3571-3670 sentence denotes A nested PCR strategy was used to genotype embryos prior to implantation as described in Figure 4.
T22646 3572-3573 DT denotes A
T22648 3574-3580 VBN denotes nested
T22649 3581-3584 NN denotes PCR
T22647 3585-3593 NN denotes strategy
T22651 3594-3597 VBD denotes was
T22650 3598-3602 VBN denotes used
T22652 3603-3605 TO denotes to
T22653 3606-3614 VB denotes genotype
T22654 3615-3622 NNS denotes embryos
T22655 3623-3628 JJ denotes prior
T22656 3629-3631 IN denotes to
T22657 3632-3644 NN denotes implantation
T22658 3645-3647 IN denotes as
T22659 3648-3657 VBN denotes described
T22660 3658-3660 IN denotes in
T22661 3661-3667 NN denotes Figure
T22662 3668-3669 CD denotes 4
T22663 3669-3670 . denotes .
T22664 3670-3773 sentence denotes Capn2+/- mice were mated and the date of fertilization established by the appearance of vaginal plugs.
T22665 3671-3676 NN denotes Capn2
T22667 3676-3677 SYM denotes +
T22668 3677-3678 HYPH denotes /
T22669 3678-3679 SYM denotes -
T22666 3680-3684 NNS denotes mice
T22671 3685-3689 VBD denotes were
T22670 3690-3695 VBN denotes mated
T22672 3696-3699 CC denotes and
T22673 3700-3703 DT denotes the
T22674 3704-3708 NN denotes date
T22676 3709-3711 IN denotes of
T22677 3712-3725 NN denotes fertilization
T22675 3726-3737 VBN denotes established
T22678 3738-3740 IN denotes by
T22679 3741-3744 DT denotes the
T22680 3745-3755 NN denotes appearance
T22681 3756-3758 IN denotes of
T22682 3759-3766 JJ denotes vaginal
T22683 3767-3772 NNS denotes plugs
T22684 3772-3773 . denotes .
T22685 3773-3884 sentence denotes Blastocyst (E3.5) or 8-cell embryos (E2.5) were flushed from the oviducts and then digested with proteinase K.
T22686 3774-3784 NN denotes Blastocyst
T22688 3785-3786 -LRB- denotes (
T22689 3786-3790 NN denotes E3.5
T22690 3790-3791 -RRB- denotes )
T22691 3792-3794 CC denotes or
T22692 3795-3796 CD denotes 8
T22694 3796-3797 HYPH denotes -
T22693 3797-3801 NN denotes cell
T22687 3802-3809 NNS denotes embryos
T22696 3810-3811 -LRB- denotes (
T22697 3811-3815 NN denotes E2.5
T22698 3815-3816 -RRB- denotes )
T22699 3817-3821 VBD denotes were
T22695 3822-3829 VBN denotes flushed
T22700 3830-3834 IN denotes from
T22701 3835-3838 DT denotes the
T22702 3839-3847 NNS denotes oviducts
T22703 3848-3851 CC denotes and
T22704 3852-3856 RB denotes then
T22705 3857-3865 VBN denotes digested
T22706 3866-3870 IN denotes with
T22707 3871-3881 NN denotes proteinase
T22708 3882-3883 NN denotes K
T22709 3883-3884 . denotes .
T22710 3884-4121 sentence denotes In separate reactions segments found exclusively in either the wild-type or mutant alleles were co-amplified with an internal control sequence, located in the short (upstream) arm of the targeting vector, which is found in both alleles.
T22711 3885-3887 IN denotes In
T22713 3888-3896 JJ denotes separate
T22715 3897-3906 NNS denotes reactions
T22714 3907-3915 NNS denotes segments
T22716 3916-3921 VBN denotes found
T22717 3922-3933 RB denotes exclusively
T22718 3934-3936 IN denotes in
T22719 3937-3943 CC denotes either
T22721 3944-3947 DT denotes the
T22722 3948-3952 JJ denotes wild
T22723 3952-3953 HYPH denotes -
T22720 3953-3957 NN denotes type
T22724 3958-3960 CC denotes or
T22725 3961-3967 NN denotes mutant
T22726 3968-3975 NNS denotes alleles
T22727 3976-3980 VBD denotes were
T22712 3981-3993 VBN denotes co-amplified
T22728 3994-3998 IN denotes with
T22729 3999-4001 DT denotes an
T22731 4002-4010 JJ denotes internal
T22732 4011-4018 NN denotes control
T22730 4019-4027 NN denotes sequence
T22733 4027-4029 , denotes ,
T22734 4029-4036 VBN denotes located
T22735 4037-4039 IN denotes in
T22736 4040-4043 DT denotes the
T22738 4044-4049 JJ denotes short
T22739 4050-4051 -LRB- denotes (
T22740 4051-4059 JJ denotes upstream
T22741 4059-4060 -RRB- denotes )
T22737 4061-4064 NN denotes arm
T22742 4065-4067 IN denotes of
T22743 4068-4071 DT denotes the
T22745 4072-4081 NN denotes targeting
T22744 4082-4088 NN denotes vector
T22746 4088-4090 , denotes ,
T22747 4090-4095 WDT denotes which
T22749 4096-4098 VBZ denotes is
T22748 4099-4104 VBN denotes found
T22750 4105-4107 IN denotes in
T22751 4108-4112 DT denotes both
T22752 4113-4120 NNS denotes alleles
T22753 4120-4121 . denotes .
T22754 4121-4245 sentence denotes The final products were 429 bp for the wild-type allele, 389 bp for the mutant allele, and 213 bp for the internal control.
T22755 4122-4125 DT denotes The
T22757 4126-4131 JJ denotes final
T22756 4132-4140 NNS denotes products
T22758 4141-4145 VBD denotes were
T22759 4146-4149 CD denotes 429
T22760 4150-4152 NN denotes bp
T22761 4153-4156 IN denotes for
T22762 4157-4160 DT denotes the
T22764 4161-4165 JJ denotes wild
T22766 4165-4166 HYPH denotes -
T22765 4166-4170 NN denotes type
T22763 4171-4177 NN denotes allele
T22767 4177-4179 , denotes ,
T22768 4179-4182 CD denotes 389
T22769 4183-4185 NN denotes bp
T22770 4186-4189 IN denotes for
T22771 4190-4193 DT denotes the
T22773 4194-4200 NN denotes mutant
T22772 4201-4207 NN denotes allele
T22774 4207-4209 , denotes ,
T22775 4209-4212 CC denotes and
T22776 4213-4216 CD denotes 213
T22777 4217-4219 NN denotes bp
T22778 4220-4223 IN denotes for
T22779 4224-4227 DT denotes the
T22781 4228-4236 JJ denotes internal
T22780 4237-4244 NN denotes control
T22782 4244-4245 . denotes .
T22783 4245-4321 sentence denotes (A) A representative example of the genotyping of blastocyst stage embryos.
T22784 4246-4247 -LRB- denotes (
T22785 4247-4248 LS denotes A
T22787 4248-4249 -RRB- denotes )
T22788 4250-4251 DT denotes A
T22789 4252-4266 JJ denotes representative
T22786 4267-4274 NN denotes example
T22790 4275-4277 IN denotes of
T22791 4278-4281 DT denotes the
T22792 4282-4292 NN denotes genotyping
T22793 4293-4295 IN denotes of
T22794 4296-4306 NN denotes blastocyst
T22796 4307-4312 NN denotes stage
T22795 4313-4320 NNS denotes embryos
T22797 4320-4321 . denotes .
T22798 4321-4455 sentence denotes Embryos #1, 2, 4, 5, and 6 were Capn2+/- whereas embryos #3 and #7 were Capn2+/+, denoted by the absence of the 389 bp mutant signal.
T22799 4322-4329 NNS denotes Embryos
T22801 4330-4331 SYM denotes #
T22800 4331-4332 CD denotes 1
T22803 4332-4334 , denotes ,
T22804 4334-4335 CD denotes 2
T22805 4335-4337 , denotes ,
T22806 4337-4338 CD denotes 4
T22807 4338-4340 , denotes ,
T22808 4340-4341 CD denotes 5
T22809 4341-4343 , denotes ,
T22810 4343-4346 CC denotes and
T22811 4347-4348 CD denotes 6
T22802 4349-4353 VBD denotes were
T22812 4354-4359 NN denotes Capn2
T22813 4359-4360 SYM denotes +
T22814 4360-4361 HYPH denotes /
T22815 4361-4362 SYM denotes -
T22816 4363-4370 IN denotes whereas
T22818 4371-4378 NNS denotes embryos
T22820 4379-4380 SYM denotes #
T22819 4380-4381 CD denotes 3
T22821 4382-4385 CC denotes and
T22822 4386-4387 SYM denotes #
T22823 4387-4388 CD denotes 7
T22817 4389-4393 VBD denotes were
T22824 4394-4399 NN denotes Capn2
T22825 4399-4400 SYM denotes +
T22826 4400-4401 HYPH denotes /
T22827 4401-4402 SYM denotes +
T22828 4402-4404 , denotes ,
T22829 4404-4411 VBN denotes denoted
T22830 4412-4414 IN denotes by
T22831 4415-4418 DT denotes the
T22832 4419-4426 NN denotes absence
T22833 4427-4429 IN denotes of
T22834 4430-4433 DT denotes the
T22836 4434-4437 CD denotes 389
T22837 4438-4440 NN denotes bp
T22838 4441-4447 NN denotes mutant
T22835 4448-4454 NN denotes signal
T22839 4454-4455 . denotes .
T22840 4455-4516 sentence denotes (B) An example of the genotyping of 8-cell embryos is shown.
T22841 4456-4457 -LRB- denotes (
T22842 4457-4458 LS denotes B
T22844 4458-4459 -RRB- denotes )
T22845 4460-4462 DT denotes An
T22846 4463-4470 NN denotes example
T22847 4471-4473 IN denotes of
T22848 4474-4477 DT denotes the
T22849 4478-4488 NN denotes genotyping
T22850 4489-4491 IN denotes of
T22851 4492-4493 CD denotes 8
T22853 4493-4494 HYPH denotes -
T22852 4494-4498 NN denotes cell
T22854 4499-4506 NNS denotes embryos
T22855 4507-4509 VBZ denotes is
T22843 4510-4515 VBN denotes shown
T22856 4515-4516 . denotes .
T22857 4516-4641 sentence denotes Embryos #1, 2, 3, 5, and 6 were Capn2+/- while embryo #4 was Capn2-/-, marked by the absence of the 429 bp wild-type signal.
T22858 4517-4524 NNS denotes Embryos
T22860 4525-4526 SYM denotes #
T22859 4526-4527 CD denotes 1
T22862 4527-4529 , denotes ,
T22863 4529-4530 CD denotes 2
T22864 4530-4532 , denotes ,
T22865 4532-4533 CD denotes 3
T22866 4533-4535 , denotes ,
T22867 4535-4536 CD denotes 5
T22868 4536-4538 , denotes ,
T22869 4538-4541 CC denotes and
T22870 4542-4543 CD denotes 6
T22861 4544-4548 VBD denotes were
T22871 4549-4554 NN denotes Capn2
T22872 4554-4555 SYM denotes +
T22873 4555-4556 HYPH denotes /
T22874 4556-4557 SYM denotes -
T22875 4558-4563 IN denotes while
T22877 4564-4570 NN denotes embryo
T22878 4571-4572 SYM denotes #
T22879 4572-4573 CD denotes 4
T22876 4574-4577 VBD denotes was
T22880 4578-4583 NN denotes Capn2
T22881 4583-4584 SYM denotes -
T22882 4584-4585 HYPH denotes /
T22883 4585-4586 SYM denotes -
T22884 4586-4588 , denotes ,
T22885 4588-4594 VBN denotes marked
T22886 4595-4597 IN denotes by
T22887 4598-4601 DT denotes the
T22888 4602-4609 NN denotes absence
T22889 4610-4612 IN denotes of
T22890 4613-4616 DT denotes the
T22892 4617-4620 CD denotes 429
T22893 4621-4623 NN denotes bp
T22894 4624-4628 JJ denotes wild
T22896 4628-4629 HYPH denotes -
T22895 4629-4633 NN denotes type
T22891 4634-4640 NN denotes signal
T22897 4640-4641 . denotes .
T22898 4641-4682 sentence denotes (M) denotes the molecular weight marker.
T22899 4642-4643 -LRB- denotes (
T22900 4643-4644 NN denotes M
T22902 4644-4645 -RRB- denotes )
T22901 4646-4653 VBZ denotes denotes
T22903 4654-4657 DT denotes the
T22905 4658-4667 JJ denotes molecular
T22906 4668-4674 NN denotes weight
T22904 4675-4681 NN denotes marker
T22907 4681-4682 . denotes .
T7006 4687-4697 NN denotes genotyping
T7008 4698-4705 VBZ denotes results
T7009 4706-4709 IN denotes for
T7010 4710-4719 NNS denotes weanlings
T7011 4719-4721 , denotes ,
T7012 4721-4738 JJ denotes post-implantation
T7014 4738-4740 , denotes ,
T7015 4740-4743 CC denotes and
T7016 4744-4760 JJ denotes pre-implantation
T7013 4761-4768 NNS denotes embryos
T7017 4769-4772 VBP denotes are
T7007 4773-4778 VBN denotes shown
T7018 4779-4781 IN denotes in
T7019 4782-4787 NN denotes Table
T7020 4788-4789 CD denotes 2
T7021 4789-4790 . denotes .
T7022 4790-4943 sentence denotes Curiously, the ratio of Capn2+/+ to Capn2+/-animals from inbred heterozygous intercrosses was substantially less than the predicted 1:2 Mendelian ratio.
T7023 4791-4800 RB denotes Curiously
T7025 4800-4802 , denotes ,
T7026 4802-4805 DT denotes the
T7027 4806-4811 NN denotes ratio
T7028 4812-4814 IN denotes of
T7029 4815-4820 NN denotes Capn2
T7030 4820-4821 SYM denotes +
T7031 4821-4822 HYPH denotes /
T7032 4822-4823 SYM denotes +
T7033 4824-4826 IN denotes to
T7034 4827-4832 NN denotes Capn2
T7036 4832-4833 SYM denotes +
T7037 4833-4834 HYPH denotes /
T7038 4834-4835 SYM denotes -
T7035 4835-4842 NNS denotes animals
T7039 4843-4847 IN denotes from
T7040 4848-4854 JJ denotes inbred
T7042 4855-4867 JJ denotes heterozygous
T7041 4868-4880 NNS denotes intercrosses
T7024 4881-4884 VBD denotes was
T7043 4885-4898 RB denotes substantially
T7044 4899-4903 JJR denotes less
T7045 4904-4908 IN denotes than
T7046 4909-4912 DT denotes the
T7048 4913-4922 VBN denotes predicted
T7049 4923-4924 CD denotes 1
T7050 4924-4925 SYM denotes :
T7051 4925-4926 CD denotes 2
T7052 4927-4936 JJ denotes Mendelian
T7047 4937-4942 NN denotes ratio
T7053 4942-4943 . denotes .
T7054 4943-5086 sentence denotes In a group of 199 animals derived from heterozygote breeding (33 litters), 23 Capn2+/+ (11.6%) and 176 (88.4%) Capn2+/- animals were observed.
T7055 4944-4946 IN denotes In
T7057 4947-4948 DT denotes a
T7058 4949-4954 NN denotes group
T7059 4955-4957 IN denotes of
T7060 4958-4961 CD denotes 199
T7061 4962-4969 NNS denotes animals
T7062 4970-4977 VBN denotes derived
T7063 4978-4982 IN denotes from
T7064 4983-4995 NN denotes heterozygote
T7065 4996-5004 NN denotes breeding
T7066 5005-5006 -LRB- denotes (
T7068 5006-5008 CD denotes 33
T7067 5009-5016 NNS denotes litters
T7069 5016-5017 -RRB- denotes )
T7070 5017-5019 , denotes ,
T7071 5019-5021 CD denotes 23
T7072 5022-5027 NN denotes Capn2
T7073 5027-5028 SYM denotes +
T7074 5028-5029 HYPH denotes /
T7075 5029-5030 SYM denotes +
T7076 5031-5032 -LRB- denotes (
T7078 5032-5036 CD denotes 11.6
T7077 5036-5037 NN denotes %
T7079 5037-5038 -RRB- denotes )
T7080 5039-5042 CC denotes and
T7081 5043-5046 CD denotes 176
T7083 5047-5048 -LRB- denotes (
T7085 5048-5052 CD denotes 88.4
T7084 5052-5053 NN denotes %
T7086 5053-5054 -RRB- denotes )
T7082 5055-5060 NN denotes Capn2
T7088 5060-5061 SYM denotes +
T7089 5061-5062 HYPH denotes /
T7090 5062-5063 SYM denotes -
T7087 5064-5071 NNS denotes animals
T7091 5072-5076 VBD denotes were
T7056 5077-5085 VBN denotes observed
T7092 5085-5086 . denotes .
T7093 5086-5335 sentence denotes The ratio of Capn2+/+ to Capn2+/- animals among males (14% to 82%) or females (13% to 90%) was essentially the same is it was for the combined population, and there were an average of six pups per litter, which is normal for this background strain.
T7094 5087-5090 DT denotes The
T7095 5091-5096 NN denotes ratio
T7097 5097-5099 IN denotes of
T7098 5100-5105 NN denotes Capn2
T7099 5105-5106 SYM denotes +
T7100 5106-5107 HYPH denotes /
T7101 5107-5108 SYM denotes +
T7102 5109-5111 IN denotes to
T7103 5112-5117 NN denotes Capn2
T7105 5117-5118 SYM denotes +
T7106 5118-5119 HYPH denotes /
T7107 5119-5120 SYM denotes -
T7104 5121-5128 NNS denotes animals
T7108 5129-5134 IN denotes among
T7109 5135-5140 NNS denotes males
T7110 5141-5142 -LRB- denotes (
T7112 5142-5144 CD denotes 14
T7111 5144-5145 NN denotes %
T7113 5146-5148 IN denotes to
T7114 5149-5151 CD denotes 82
T7115 5151-5152 NN denotes %
T7116 5152-5153 -RRB- denotes )
T7117 5154-5156 CC denotes or
T7118 5157-5164 NNS denotes females
T7119 5165-5166 -LRB- denotes (
T7121 5166-5168 CD denotes 13
T7120 5168-5169 NN denotes %
T7122 5170-5172 IN denotes to
T7123 5173-5175 CD denotes 90
T7124 5175-5176 NN denotes %
T7125 5176-5177 -RRB- denotes )
T7096 5178-5181 VBD denotes was
T7126 5182-5193 RB denotes essentially
T7127 5194-5197 DT denotes the
T7128 5198-5202 JJ denotes same
T7129 5203-5205 IN denotes is
T7131 5206-5208 PRP denotes it
T7130 5209-5212 VBD denotes was
T7132 5213-5216 IN denotes for
T7133 5217-5220 DT denotes the
T7135 5221-5229 VBN denotes combined
T7134 5230-5240 NN denotes population
T7136 5240-5242 , denotes ,
T7137 5242-5245 CC denotes and
T7138 5246-5251 EX denotes there
T7139 5252-5256 VBD denotes were
T7140 5257-5259 DT denotes an
T7141 5260-5267 NN denotes average
T7142 5268-5270 IN denotes of
T7143 5271-5274 CD denotes six
T7144 5275-5279 NNS denotes pups
T7145 5280-5283 IN denotes per
T7146 5284-5290 NN denotes litter
T7147 5290-5292 , denotes ,
T7148 5292-5297 WDT denotes which
T7149 5298-5300 VBZ denotes is
T7150 5301-5307 JJ denotes normal
T7151 5308-5311 IN denotes for
T7152 5312-5316 DT denotes this
T7154 5317-5327 NN denotes background
T7153 5328-5334 NN denotes strain
T7155 5334-5335 . denotes .
T7156 5335-5469 sentence denotes A larger than expected proportion Capn2+/- animals was also evident in both post-implantation and pre-implantation embryos (Table 2).
T7157 5336-5337 DT denotes A
T7159 5338-5344 JJR denotes larger
T7160 5345-5349 IN denotes than
T7161 5350-5358 VBN denotes expected
T7158 5359-5369 NN denotes proportion
T7163 5370-5375 NN denotes Capn2
T7165 5375-5376 SYM denotes +
T7166 5376-5377 HYPH denotes /
T7167 5377-5378 SYM denotes -
T7164 5379-5386 NNS denotes animals
T7162 5387-5390 VBD denotes was
T7168 5391-5395 RB denotes also
T7169 5396-5403 JJ denotes evident
T7170 5404-5406 IN denotes in
T7171 5407-5411 CC denotes both
T7173 5412-5429 JJ denotes post-implantation
T7174 5430-5433 CC denotes and
T7175 5434-5450 JJ denotes pre-implantation
T7172 5451-5458 NNS denotes embryos
T7176 5459-5460 -LRB- denotes (
T7177 5460-5465 NN denotes Table
T7178 5466-5467 CD denotes 2
T7179 5467-5468 -RRB- denotes )
T7180 5468-5469 . denotes .
T7181 5469-5647 sentence denotes Interestingly, a similar over-representation of heterozygous animals was also reported in one of the Capn4 transgenic lines, though the genotype skewing was not as extreme [32].
T7182 5470-5483 RB denotes Interestingly
T7184 5483-5485 , denotes ,
T7185 5485-5486 DT denotes a
T7187 5487-5494 JJ denotes similar
T7186 5495-5514 NN denotes over-representation
T7188 5515-5517 IN denotes of
T7189 5518-5530 JJ denotes heterozygous
T7190 5531-5538 NNS denotes animals
T7191 5539-5542 VBD denotes was
T7192 5543-5547 RB denotes also
T7183 5548-5556 VBN denotes reported
T7193 5557-5559 IN denotes in
T7194 5560-5563 CD denotes one
T7195 5564-5566 IN denotes of
T7196 5567-5570 DT denotes the
T7198 5571-5576 NN denotes Capn4
T7199 5577-5587 JJ denotes transgenic
T7197 5588-5593 NNS denotes lines
T7200 5593-5595 , denotes ,
T7201 5595-5601 IN denotes though
T7203 5602-5605 DT denotes the
T7205 5606-5614 NN denotes genotype
T7204 5615-5622 NN denotes skewing
T7202 5623-5626 VBD denotes was
T7206 5627-5630 RB denotes not
T7207 5631-5633 IN denotes as
T7208 5634-5641 JJ denotes extreme
T7209 5642-5643 -LRB- denotes [
T7210 5643-5645 CD denotes 32
T7211 5645-5646 -RRB- denotes ]
T7212 5646-5647 . denotes .
T7213 5647-5776 sentence denotes Crosses between Capn2+/+ and Capn2+/- animals also resulted in a greater than expected proportion of Capn2+/- animals (Table 2).
T7214 5648-5655 NNS denotes Crosses
T7216 5656-5663 IN denotes between
T7217 5664-5669 NN denotes Capn2
T7219 5669-5670 SYM denotes +
T7220 5670-5671 HYPH denotes /
T7221 5671-5672 SYM denotes +
T7222 5673-5676 CC denotes and
T7223 5677-5682 NN denotes Capn2
T7224 5682-5683 SYM denotes +
T7225 5683-5684 HYPH denotes /
T7226 5684-5685 SYM denotes -
T7218 5686-5693 NNS denotes animals
T7227 5694-5698 RB denotes also
T7215 5699-5707 VBD denotes resulted
T7228 5708-5710 IN denotes in
T7229 5711-5712 DT denotes a
T7231 5713-5720 JJR denotes greater
T7232 5721-5725 IN denotes than
T7233 5726-5734 VBN denotes expected
T7230 5735-5745 NN denotes proportion
T7234 5746-5748 IN denotes of
T7235 5749-5754 NN denotes Capn2
T7237 5754-5755 SYM denotes +
T7238 5755-5756 HYPH denotes /
T7239 5756-5757 SYM denotes -
T7236 5758-5765 NNS denotes animals
T7240 5766-5767 -LRB- denotes (
T7241 5767-5772 NN denotes Table
T7242 5773-5774 CD denotes 2
T7243 5774-5775 -RRB- denotes )
T7244 5775-5776 . denotes .
T7245 5776-5932 sentence denotes An even higher degree of Capn2+/- over-representation was seen when the mutant allele came the mother (73%) compared to when it came from the father (59%).
T7246 5777-5779 DT denotes An
T7248 5780-5784 RB denotes even
T7249 5785-5791 JJR denotes higher
T7247 5792-5798 NN denotes degree
T7251 5799-5801 IN denotes of
T7252 5802-5807 NN denotes Capn2
T7254 5807-5808 SYM denotes +
T7255 5808-5809 HYPH denotes /
T7256 5809-5810 SYM denotes -
T7253 5811-5830 NN denotes over-representation
T7257 5831-5834 VBD denotes was
T7250 5835-5839 VBN denotes seen
T7258 5840-5844 WRB denotes when
T7260 5845-5848 DT denotes the
T7262 5849-5855 NN denotes mutant
T7261 5856-5862 NN denotes allele
T7259 5863-5867 VBD denotes came
T7263 5868-5871 DT denotes the
T7264 5872-5878 NN denotes mother
T7265 5879-5880 -LRB- denotes (
T7267 5880-5882 CD denotes 73
T7266 5882-5883 NN denotes %
T7268 5883-5884 -RRB- denotes )
T7269 5885-5893 VBN denotes compared
T7270 5894-5896 IN denotes to
T7271 5897-5901 WRB denotes when
T7273 5902-5904 PRP denotes it
T7272 5905-5909 VBD denotes came
T7274 5910-5914 IN denotes from
T7275 5915-5918 DT denotes the
T7276 5919-5925 NN denotes father
T7277 5926-5927 -LRB- denotes (
T7279 5927-5929 CD denotes 59
T7278 5929-5930 NN denotes %
T7280 5930-5931 -RRB- denotes )
T7281 5931-5932 . denotes .
T7282 5932-6259 sentence denotes In these crosses the ratios of Capn2+/+ to Capn2+/- animals among males or females compared well with the ratio in the combined populations; 77% of males and 69% of females were Capn2+/- when the mutant allele came from the mother, and 62% of males and 55% of females were Capn2+/- when the mutant allele came from the father.
T7283 5933-5935 IN denotes In
T7285 5936-5941 DT denotes these
T7286 5942-5949 NNS denotes crosses
T7287 5950-5953 DT denotes the
T7288 5954-5960 NNS denotes ratios
T7289 5961-5963 IN denotes of
T7290 5964-5969 NN denotes Capn2
T7291 5969-5970 SYM denotes +
T7292 5970-5971 HYPH denotes /
T7293 5971-5972 SYM denotes +
T7294 5973-5975 IN denotes to
T7295 5976-5981 NN denotes Capn2
T7297 5981-5982 SYM denotes +
T7298 5982-5983 HYPH denotes /
T7299 5983-5984 SYM denotes -
T7296 5985-5992 NNS denotes animals
T7300 5993-5998 IN denotes among
T7301 5999-6004 NNS denotes males
T7302 6005-6007 CC denotes or
T7303 6008-6015 NNS denotes females
T7284 6016-6024 VBN denotes compared
T7304 6025-6029 RB denotes well
T7305 6030-6034 IN denotes with
T7306 6035-6038 DT denotes the
T7307 6039-6044 NN denotes ratio
T7308 6045-6047 IN denotes in
T7309 6048-6051 DT denotes the
T7311 6052-6060 VBN denotes combined
T7310 6061-6072 NNS denotes populations
T7312 6072-6073 : denotes ;
T7313 6074-6076 CD denotes 77
T7314 6076-6077 NN denotes %
T7316 6078-6080 IN denotes of
T7317 6081-6086 NNS denotes males
T7318 6087-6090 CC denotes and
T7319 6091-6093 CD denotes 69
T7320 6093-6094 NN denotes %
T7321 6095-6097 IN denotes of
T7322 6098-6105 NNS denotes females
T7315 6106-6110 VBD denotes were
T7323 6111-6116 NN denotes Capn2
T7324 6116-6117 SYM denotes +
T7325 6117-6118 HYPH denotes /
T7326 6118-6119 SYM denotes -
T7327 6120-6124 WRB denotes when
T7329 6125-6128 DT denotes the
T7331 6129-6135 NN denotes mutant
T7330 6136-6142 NN denotes allele
T7328 6143-6147 VBD denotes came
T7332 6148-6152 IN denotes from
T7333 6153-6156 DT denotes the
T7334 6157-6163 NN denotes mother
T7335 6163-6165 , denotes ,
T7336 6165-6168 CC denotes and
T7337 6169-6171 CD denotes 62
T7338 6171-6172 NN denotes %
T7340 6173-6175 IN denotes of
T7341 6176-6181 NNS denotes males
T7342 6182-6185 CC denotes and
T7343 6186-6188 CD denotes 55
T7344 6188-6189 NN denotes %
T7345 6190-6192 IN denotes of
T7346 6193-6200 NNS denotes females
T7339 6201-6205 VBD denotes were
T7347 6206-6211 NN denotes Capn2
T7348 6211-6212 SYM denotes +
T7349 6212-6213 HYPH denotes /
T7350 6213-6214 SYM denotes -
T7351 6215-6219 WRB denotes when
T7353 6220-6223 DT denotes the
T7355 6224-6230 NN denotes mutant
T7354 6231-6237 NN denotes allele
T7352 6238-6242 VBD denotes came
T7356 6243-6247 IN denotes from
T7357 6248-6251 DT denotes the
T7358 6252-6258 NN denotes father
T7359 6258-6259 . denotes .
R1543 T6636 T6635 prep of,Generation
R1544 T6637 T6638 amod chimeric,mice
R1545 T6638 T6636 pobj mice,of
R1546 T6639 T6635 cc and,Generation
R1547 T6640 T6641 compound germline,transmission
R1548 T6641 T6635 conj transmission,Generation
R1549 T6642 T6641 prep of,transmission
R1550 T6643 T6644 det the,allele
R1551 T6644 T6642 pobj allele,of
R1552 T6645 T6644 compound mutant,allele
R1553 T6646 T6644 compound Capn2,allele
R1554 T6648 T6649 nummod Eight,mice
R1555 T6649 T6652 nsubjpass mice,produced
R1556 T6650 T6649 amod chimeric,mice
R1557 T6651 T6649 amod male,mice
R1558 T6653 T6652 auxpass were,produced
R1559 T6654 T6652 prep in,produced
R1560 T6655 T6656 compound morula,aggregation
R1561 T6656 T6657 compound aggregation,experiments
R1562 T6657 T6654 pobj experiments,in
R1563 T6658 T6652 advcl using,produced
R1564 T6659 T6660 det the,line
R1565 T6660 T6658 dobj line,using
R1566 T6661 T6660 nmod Capn2,line
R1567 T6662 T6661 punct +,Capn2
R1568 T6663 T6661 punct /,Capn2
R1569 T6664 T6661 punct -,Capn2
R1570 T6665 T6660 compound ES27,line
R1571 T6666 T6660 compound cell,line
R1572 T6667 T6652 punct .,produced
R1573 T6669 T6670 nsubj Two,transmitted
R1574 T6671 T6669 prep of,Two
R1575 T6672 T6673 det these,males
R1576 T6673 T6671 pobj males,of
R1577 T6674 T6675 det the,genotype
R1578 T6675 T6670 dobj genotype,transmitted
R1579 T6676 T6675 nmod Capn2,genotype
R1580 T6677 T6676 punct +,Capn2
R1581 T6678 T6676 punct /,Capn2
R1582 T6679 T6676 punct -,Capn2
R1583 T6680 T6675 compound ES27,genotype
R1584 T6681 T6670 prep through,transmitted
R1585 T6682 T6683 det the,germline
R1586 T6683 T6681 pobj germline,through
R1587 T6684 T6670 prep into,transmitted
R1588 T6685 T6686 det the,generation
R1589 T6686 T6684 pobj generation,into
R1590 T6687 T6686 compound F1,generation
R1591 T6688 T6670 punct .,transmitted
R1592 T6690 T6691 amod Heterozygous,animals
R1593 T6691 T6696 nsubj animals,appeared
R1594 T6692 T6691 nmod Capn2,animals
R1595 T6693 T6692 punct +,Capn2
R1596 T6694 T6692 punct /,Capn2
R1597 T6695 T6692 punct -,Capn2
R1598 T6697 T6696 oprd normal,appeared
R1599 T6698 T6696 punct ", ",appeared
R1600 T6699 T6696 prep with,appeared
R1601 T6700 T6701 det no,defects
R1602 T6701 T6699 pobj defects,with
R1603 T6702 T6701 amod obvious,defects
R1604 T6703 T6701 prep in,defects
R1605 T6704 T6705 amod gross,anatomy
R1606 T6705 T6703 pobj anatomy,in
R1607 T6706 T6705 punct ", ",anatomy
R1608 T6707 T6705 conj reproduction,anatomy
R1609 T6708 T6707 punct ", ",reproduction
R1610 T6709 T6707 cc or,reproduction
R1611 T6710 T6711 compound life,span
R1612 T6711 T6707 conj span,reproduction
R1613 T6712 T6696 punct .,appeared
R1614 T6714 T6715 prep Out,detected
R1615 T6716 T6714 prep of,Out
R1616 T6717 T6718 nummod 199,weanlings
R1617 T6718 T6716 pobj weanlings,of
R1618 T6719 T6718 prep from,weanlings
R1619 T6720 T6721 amod heterozygous,intercrosses
R1620 T6721 T6719 pobj intercrosses,from
R1621 T6722 T6715 punct ", ",detected
R1622 T6723 T6724 det no,progeny
R1623 T6724 T6715 nsubjpass progeny,detected
R1624 T6725 T6724 nmod Capn2,progeny
R1625 T6726 T6725 punct -,Capn2
R1626 T6727 T6725 punct /,Capn2
R1627 T6728 T6725 punct -,Capn2
R1628 T6729 T6715 auxpass were,detected
R1629 T6730 T6731 punct (,Table
R1630 T6731 T6715 parataxis Table,detected
R1631 T6732 T6731 nummod 2,Table
R1632 T6733 T6731 punct ),Table
R1633 T6734 T6715 punct .,detected
R1634 T6736 T6737 nsubj We,observe
R1635 T6738 T6737 aux did,observe
R1636 T6739 T6737 neg not,observe
R1637 T6740 T6741 amod high,rates
R1638 T6741 T6737 dobj rates,observe
R1639 T6742 T6741 prep of,rates
R1640 T6743 T6744 amod perinatal,death
R1641 T6744 T6742 pobj death,of
R1642 T6745 T6737 punct ", ",observe
R1643 T6746 T6737 cc and,observe
R1644 T6747 T6748 det no,stillborns
R1645 T6748 T6753 nsubjpass stillborns,observed
R1646 T6749 T6748 nmod Capn2,stillborns
R1647 T6750 T6749 punct -,Capn2
R1648 T6751 T6749 punct /,Capn2
R1649 T6752 T6749 punct -,Capn2
R1650 T6753 T6737 conj observed,observe
R1651 T6754 T6753 auxpass were,observed
R1652 T6755 T6753 punct .,observed
R1653 T6757 T6758 nsubj This,suggested
R1654 T6759 T6760 mark that,perished
R1655 T6760 T6758 ccomp perished,suggested
R1656 T6761 T6762 nmod Capn2,animals
R1657 T6762 T6760 nsubj animals,perished
R1658 T6763 T6761 punct -,Capn2
R1659 T6764 T6761 punct /,Capn2
R1660 T6765 T6761 punct -,Capn2
R1661 T6766 T6760 prep at,perished
R1662 T6767 T6768 det some,stage
R1663 T6768 T6766 pobj stage,at
R1664 T6769 T6768 prep during,stage
R1665 T6770 T6771 amod embryonic,development
R1666 T6771 T6769 pobj development,during
R1667 T6772 T6758 punct .,suggested
R1668 T6774 T6775 prep In,harvested
R1669 T6776 T6777 det an,attempt
R1670 T6777 T6774 pobj attempt,In
R1671 T6778 T6779 aux to,determine
R1672 T6779 T6777 acl determine,attempt
R1673 T6780 T6781 mark if,occurred
R1674 T6781 T6779 ccomp occurred,determine
R1675 T6782 T6783 amod embryonic,death
R1676 T6783 T6781 nsubj death,occurred
R1677 T6784 T6781 prep at,occurred
R1678 T6785 T6786 det a,stage
R1679 T6786 T6784 pobj stage,at
R1680 T6787 T6786 amod post-implantation,stage
R1681 T6788 T6775 punct ", ",harvested
R1682 T6789 T6775 nsubjpass embryos,harvested
R1683 T6790 T6775 auxpass were,harvested
R1684 T6791 T6775 prep for,harvested
R1685 T6792 T6791 pobj genotyping,for
R1686 T6793 T6775 prep at,harvested
R1687 T6794 T6795 amod different,times
R1688 T6795 T6793 pobj times,at
R1689 T6796 T6795 prep between,times
R1690 T6797 T6796 pobj E10.5,between
R1691 T6798 T6797 cc and,E10.5
R1692 T6799 T6797 conj E18.5,E10.5
R1693 T6800 T6775 punct .,harvested
R1694 T6802 T6803 det No,embryos
R1695 T6803 T6808 nsubjpass embryos,observed
R1696 T6804 T6803 nmod Capn2,embryos
R1697 T6805 T6804 punct -,Capn2
R1698 T6806 T6804 punct /,Capn2
R1699 T6807 T6804 punct -,Capn2
R1700 T6809 T6808 auxpass were,observed
R1701 T6810 T6808 cc and,observed
R1702 T6811 T6812 det no,signs
R1703 T6812 T6813 nsubjpass signs,detected
R1704 T6813 T6808 conj detected,observed
R1705 T6814 T6812 prep of,signs
R1706 T6815 T6816 compound embryo,resorption
R1707 T6816 T6814 pobj resorption,of
R1708 T6817 T6813 auxpass were,detected
R1709 T6818 T6819 punct (,Table
R1710 T6819 T6813 parataxis Table,detected
R1711 T6820 T6819 nummod 2,Table
R1712 T6821 T6819 punct ),Table
R1713 T6822 T6813 punct .,detected
R1714 T6824 T6825 nsubj This,indicated
R1715 T6826 T6827 mark that,dying
R1716 T6827 T6825 ccomp dying,indicated
R1717 T6828 T6829 det the,embryos
R1718 T6829 T6827 nsubj embryos,dying
R1719 T6830 T6829 nmod Capn2,embryos
R1720 T6831 T6830 punct -,Capn2
R1721 T6832 T6830 punct /,Capn2
R1722 T6833 T6830 punct -,Capn2
R1723 T6834 T6827 aux might,dying
R1724 T6835 T6827 aux be,dying
R1725 T6836 T6837 amod prior,to
R1726 T6837 T6827 prep to,dying
R1727 T6838 T6837 pobj implantation,to
R1728 T6839 T6825 punct .,indicated
R1729 T6841 T6842 nsubjpass Embryos,flushed
R1730 T6843 T6842 auxpass were,flushed
R1731 T6844 T6842 advmod then,flushed
R1732 T6845 T6842 prep from,flushed
R1733 T6846 T6847 det the,oviducts
R1734 T6847 T6845 pobj oviducts,from
R1735 T6848 T6847 prep of,oviducts
R1736 T6849 T6850 amod pregnant,females
R1737 T6850 T6848 pobj females,of
R1738 T6851 T6842 prep at,flushed
R1739 T6852 T6851 pobj E2.5,at
R1740 T6853 T6852 cc or,E2.5
R1741 T6854 T6852 conj E3.5,E2.5
R1742 T6855 T6842 punct ", ",flushed
R1743 T6856 T6842 cc and,flushed
R1744 T6857 T6842 conj genotyped,flushed
R1745 T6858 T6857 prep by,genotyped
R1746 T6859 T6858 pobj means,by
R1747 T6860 T6859 prep of,means
R1748 T6861 T6862 det a,strategy
R1749 T6862 T6860 pobj strategy,of
R1750 T6863 T6862 amod nested,strategy
R1751 T6864 T6862 compound PCR,strategy
R1752 T6865 T6866 punct (,Figure
R1753 T6866 T6857 parataxis Figure,genotyped
R1754 T6867 T6866 nummod 4,Figure
R1755 T6868 T6866 punct ),Figure
R1756 T6869 T6842 punct .,flushed
R1757 T6871 T6872 nsubj Two,were
R1758 T6873 T6871 prep of,Two
R1759 T6874 T6875 nummod 90,embryos
R1760 T6875 T6873 pobj embryos,of
R1761 T6876 T6877 advmod successfully,genotyped
R1762 T6877 T6875 amod genotyped,embryos
R1763 T6878 T6875 amod pre-implantation,embryos
R1764 T6879 T6872 attr Capn2,were
R1765 T6880 T6879 punct -,Capn2
R1766 T6881 T6879 punct /,Capn2
R1767 T6882 T6879 punct -,Capn2
R1768 T6883 T6872 punct ", ",were
R1769 T6884 T6885 punct (,Figure
R1770 T6885 T6872 parataxis Figure,were
R1771 T6886 T6885 dep Table,Figure
R1772 T6887 T6886 nummod 2,Table
R1773 T6888 T6885 punct ;,Figure
R1774 T6889 T6885 nummod 5,Figure
R1775 T6890 T6885 punct ),Figure
R1776 T6891 T6872 punct .,were
R1777 T6893 T6894 nsubjpass Both,isolated
R1778 T6895 T6893 prep of,Both
R1779 T6896 T6897 det these,embryos
R1780 T6897 T6895 pobj embryos,of
R1781 T6898 T6897 nmod Capn2,embryos
R1782 T6899 T6898 punct -,Capn2
R1783 T6900 T6898 punct /,Capn2
R1784 T6901 T6898 punct -,Capn2
R1785 T6902 T6894 auxpass were,isolated
R1786 T6903 T6894 prep at,isolated
R1787 T6904 T6905 det the,stage
R1788 T6905 T6903 pobj stage,at
R1789 T6906 T6907 nummod 8,cell
R1790 T6907 T6905 compound cell,stage
R1791 T6908 T6907 punct -,cell
R1792 T6909 T6894 cc and,isolated
R1793 T6910 T6911 aux did,display
R1794 T6911 T6894 conj display,isolated
R1795 T6912 T6911 neg not,display
R1796 T6913 T6914 det any,defects
R1797 T6914 T6911 dobj defects,display
R1798 T6915 T6914 amod obvious,defects
R1799 T6916 T6914 amod morphological,defects
R1800 T6917 T6894 punct .,isolated
R1801 T6919 T6920 nsubj None,were
R1802 T6921 T6919 prep of,None
R1803 T6922 T6923 det the,embryos
R1804 T6923 T6921 pobj embryos,of
R1805 T6924 T6923 nummod 46,embryos
R1806 T6925 T6926 advmod successfully,genotyped
R1807 T6926 T6923 amod genotyped,embryos
R1808 T6927 T6928 npadvmod blastocyst,staged
R1809 T6928 T6923 amod staged,embryos
R1810 T6929 T6928 punct -,staged
R1811 T6930 T6920 attr Capn2,were
R1812 T6931 T6930 punct -,Capn2
R1813 T6932 T6930 punct /,Capn2
R1814 T6933 T6930 punct -,Capn2
R1815 T6934 T6920 punct .,were
R1816 T6936 T6937 det The,scarcity
R1817 T6937 T6938 nsubj scarcity,suggested
R1818 T6939 T6937 prep of,scarcity
R1819 T6940 T6941 npadvmod Capn2,deficient
R1820 T6941 T6943 amod deficient,embryos
R1821 T6942 T6941 punct -,deficient
R1822 T6943 T6939 pobj embryos,of
R1823 T6944 T6943 acl surviving,embryos
R1824 T6945 T6944 prep to,surviving
R1825 T6946 T6947 det the,stage
R1826 T6947 T6945 pobj stage,to
R1827 T6948 T6949 nummod 8,cell
R1828 T6949 T6947 compound cell,stage
R1829 T6950 T6949 punct -,cell
R1830 T6951 T6952 mark that,compromise
R1831 T6952 T6938 ccomp compromise,suggested
R1832 T6953 T6954 det the,loss
R1833 T6954 T6952 nsubj loss,compromise
R1834 T6955 T6954 prep of,loss
R1835 T6956 T6957 compound m,calpain
R1836 T6957 T6959 compound calpain,activity
R1837 T6958 T6957 punct -,calpain
R1838 T6959 T6955 pobj activity,of
R1839 T6960 T6952 aux must,compromise
R1840 T6961 T6952 advmod fatally,compromise
R1841 T6962 T6963 det the,viability
R1842 T6963 T6952 dobj viability,compromise
R1843 T6964 T6963 prep of,viability
R1844 T6965 T6966 amod early,embryos
R1845 T6966 T6964 pobj embryos,of
R1846 T6967 T6938 punct .,suggested
R1847 T6969 T6970 advmod Furthermore,is
R1848 T6971 T6970 punct ", ",is
R1849 T6972 T6970 nsubj it,is
R1850 T6973 T6970 acomp possible,is
R1851 T6974 T6975 mark that,allowed
R1852 T6975 T6970 ccomp allowed,is
R1853 T6976 T6975 nsubj persistence,allowed
R1854 T6977 T6976 prep of,persistence
R1855 T6978 T6979 det some,transcript
R1856 T6979 T6977 pobj transcript,of
R1857 T6980 T6981 advmod maternally,derived
R1858 T6981 T6979 amod derived,transcript
R1859 T6982 T6979 compound mRNA,transcript
R1860 T6983 T6979 cc or,transcript
R1861 T6984 T6979 conj protein,transcript
R1862 T6985 T6975 aux might,allowed
R1863 T6986 T6975 aux have,allowed
R1864 T6987 T6988 det a,number
R1865 T6988 T6990 nsubj number,survive
R1866 T6989 T6988 amod small,number
R1867 T6990 T6975 ccomp survive,allowed
R1868 T6991 T6988 prep of,number
R1869 T6992 T6993 nmod Capn2,embryos
R1870 T6993 T6991 pobj embryos,of
R1871 T6994 T6992 punct -,Capn2
R1872 T6995 T6992 punct /,Capn2
R1873 T6996 T6992 punct -,Capn2
R1874 T6997 T6990 aux to,survive
R1875 T6998 T6990 prep to,survive
R1876 T6999 T7000 det the,stage
R1877 T7000 T6998 pobj stage,to
R1878 T7001 T7000 compound morula,stage
R1879 T7002 T7000 punct -,stage
R1880 T7003 T6970 punct .,is
R1881 T7005 T7006 det "Table 2 Genotype distribution of offspring derived from Capn2 transgenic mice. Cross (N) Age Genotype +/+ +/- -/- ND* (+/-)♀ × (+/-)♂ 199 3 weeks 23 176 0 0 (+/+)♀ × (+/-)♂ 122 3 weeks 50 72 0 0 (+/-)♀ × (+/+)♂ 103 3 weeks 28 75 0 0 (+/-)♀ × (+/-)♂ 9 E18.5 0 7 0 2 (+/-)♀ × (+/-)♂ 5 E17.5 0 5 0 0 (+/-)♀ × (+/-)♂ 9 E14.5 2 7 0 0 (+/-)♀ × (+/-)♂ 10 E11.5 3 7 0 0 (+/-)♀ × (+/-)♂ 7 E10.5 2 5 0 0 (+/-)♀ × (+/-)♂ 46 E3.5 7 39 0 0 (+/-)♀ × (+/-)♂ 48 E2.5 8 34 2 4 * ND, not determined Figure 4 Nested PCR strategy for genotyping of pre-implantation embryos. Due to the scarcity of extractable genetic material, a nested PCR strategy was developed in order to genotype pre-implantation embryos. Separate reactions were used to amplify a 429 bp fragment from the wild-type allele and a 389 bp segment from the mutant allele, both spanning the 3' end of the short (upstream) arm of the targeting vector. In both reactions, a 213 bp sequence located within the short arm was co-amplified with the 'diagnostic' products as an internal control. The same sense primers were used to amplify 'diagnostic' sequences in both reactions, whereas the antisense primers were allele-specific. The primers, represented by triangles, are depicted in two (nested) sets for each of the three reactions. Exons are represented by open, vertical rectangles, the PGK-Neo cassette by an open, horizontal rectangle, while thin vertical lines denote the boundaries of the short arm and the 5' end of the long (downstream) arm. A grey, horizontal rectangle delineates the segment of the wild-type allele that is replaced by the PGK-Neo cassette in the mutant allele. Figure 5 Genotyping of pre-implantation embryos. A nested PCR strategy was used to genotype embryos prior to implantation as described in Figure 4. Capn2+/- mice were mated and the date of fertilization established by the appearance of vaginal plugs. Blastocyst (E3.5) or 8-cell embryos (E2.5) were flushed from the oviducts and then digested with proteinase K. In separate reactions segments found exclusively in either the wild-type or mutant alleles were co-amplified with an internal control sequence, located in the short (upstream) arm of the targeting vector, which is found in both alleles. The final products were 429 bp for the wild-type allele, 389 bp for the mutant allele, and 213 bp for the internal control. (A) A representative example of the genotyping of blastocyst stage embryos. Embryos #1, 2, 4, 5, and 6 were Capn2+/- whereas embryos #3 and #7 were Capn2+/+, denoted by the absence of the 389 bp mutant signal. (B) An example of the genotyping of 8-cell embryos is shown. Embryos #1, 2, 3, 5, and 6 were Capn2+/- while embryo #4 was Capn2-/-, marked by the absence of the 429 bp wild-type signal. (M) denotes the molecular weight marker. The",genotyping
R1882 T7006 T7007 nsubjpass genotyping,shown
R1883 T7008 T7006 nmod results,genotyping
R1884 T7009 T7006 prep for,genotyping
R1885 T7010 T7009 pobj weanlings,for
R1886 T7011 T7010 punct ", ",weanlings
R1887 T7012 T7013 nmod post-implantation,embryos
R1888 T7013 T7010 appos embryos,weanlings
R1889 T7014 T7012 punct ", ",post-implantation
R1890 T7015 T7012 cc and,post-implantation
R1891 T7016 T7012 conj pre-implantation,post-implantation
R1892 T7017 T7007 auxpass are,shown
R1893 T7018 T7007 prep in,shown
R1894 T7019 T7018 pobj Table,in
R1895 T7020 T7019 nummod 2,Table
R1896 T7021 T7007 punct .,shown
R1897 T7023 T7024 advmod Curiously,was
R1898 T7025 T7024 punct ", ",was
R1899 T7026 T7027 det the,ratio
R1900 T7027 T7024 nsubj ratio,was
R1901 T7028 T7027 prep of,ratio
R1902 T7029 T7028 pobj Capn2,of
R1903 T7030 T7029 punct +,Capn2
R1904 T7031 T7029 punct /,Capn2
R1905 T7032 T7029 punct +,Capn2
R1906 T7033 T7027 prep to,ratio
R1907 T7034 T7035 nmod Capn2,animals
R1908 T7035 T7033 pobj animals,to
R1909 T7036 T7034 punct +,Capn2
R1910 T7037 T7034 punct /,Capn2
R1911 T7038 T7034 punct -,Capn2
R1912 T7039 T7027 prep from,ratio
R1913 T7040 T7041 amod inbred,intercrosses
R1914 T7041 T7039 pobj intercrosses,from
R1915 T7042 T7041 amod heterozygous,intercrosses
R1916 T7043 T7044 advmod substantially,less
R1917 T7044 T7024 acomp less,was
R1918 T7045 T7044 prep than,less
R1919 T7046 T7047 det the,ratio
R1920 T7047 T7045 pobj ratio,than
R1921 T7048 T7047 amod predicted,ratio
R1922 T7049 T7047 nummod 1,ratio
R1923 T7050 T7051 punct :,2
R1924 T7051 T7049 prep 2,1
R1925 T7052 T7047 amod Mendelian,ratio
R1926 T7053 T7024 punct .,was
R1927 T7055 T7056 prep In,observed
R1928 T7057 T7058 det a,group
R1929 T7058 T7055 pobj group,In
R1930 T7059 T7058 prep of,group
R1931 T7060 T7061 nummod 199,animals
R1932 T7061 T7059 pobj animals,of
R1933 T7062 T7061 acl derived,animals
R1934 T7063 T7062 prep from,derived
R1935 T7064 T7065 compound heterozygote,breeding
R1936 T7065 T7063 pobj breeding,from
R1937 T7066 T7067 punct (,litters
R1938 T7067 T7065 parataxis litters,breeding
R1939 T7068 T7067 nummod 33,litters
R1940 T7069 T7067 punct ),litters
R1941 T7070 T7056 punct ", ",observed
R1942 T7071 T7072 nummod 23,Capn2
R1943 T7072 T7056 nsubjpass Capn2,observed
R1944 T7073 T7072 punct +,Capn2
R1945 T7074 T7072 punct /,Capn2
R1946 T7075 T7072 punct +,Capn2
R1947 T7076 T7077 punct (,%
R1948 T7077 T7072 parataxis %,Capn2
R1949 T7078 T7077 nummod 11.6,%
R1950 T7079 T7077 punct ),%
R1951 T7080 T7072 cc and,Capn2
R1952 T7081 T7082 nummod 176,Capn2
R1953 T7082 T7087 nmod Capn2,animals
R1954 T7083 T7084 punct (,%
R1955 T7084 T7082 parataxis %,Capn2
R1956 T7085 T7084 nummod 88.4,%
R1957 T7086 T7084 punct ),%
R1958 T7087 T7072 conj animals,Capn2
R1959 T7088 T7082 punct +,Capn2
R1960 T7089 T7082 punct /,Capn2
R1961 T7090 T7082 punct -,Capn2
R1962 T7091 T7056 auxpass were,observed
R1963 T7092 T7056 punct .,observed
R1964 T7094 T7095 det The,ratio
R1965 T7095 T7096 nsubj ratio,was
R1966 T7097 T7095 prep of,ratio
R1967 T7098 T7097 pobj Capn2,of
R1968 T7099 T7098 punct +,Capn2
R1969 T7100 T7098 punct /,Capn2
R1970 T7101 T7098 punct +,Capn2
R1971 T7102 T7095 prep to,ratio
R1972 T7103 T7104 nmod Capn2,animals
R1973 T7104 T7102 pobj animals,to
R1974 T7105 T7103 punct +,Capn2
R1975 T7106 T7103 punct /,Capn2
R1976 T7107 T7103 punct -,Capn2
R1977 T7108 T7095 prep among,ratio
R1978 T7109 T7108 pobj males,among
R1979 T7110 T7111 punct (,%
R1980 T7111 T7109 parataxis %,males
R1981 T7112 T7111 nummod 14,%
R1982 T7113 T7111 prep to,%
R1983 T7114 T7115 nummod 82,%
R1984 T7115 T7113 pobj %,to
R1985 T7116 T7111 punct ),%
R1986 T7117 T7109 cc or,males
R1987 T7118 T7109 conj females,males
R1988 T7202 T7183 advcl was,reported
R1989 T7119 T7120 punct (,%
R1990 T7203 T7204 det the,skewing
R1991 T7120 T7118 parataxis %,females
R1992 T7204 T7202 nsubj skewing,was
R1993 T7121 T7120 nummod 13,%
R1994 T7205 T7204 compound genotype,skewing
R1995 T7206 T7202 neg not,was
R1996 T7207 T7202 prep as,was
R1997 T7122 T7120 prep to,%
R1998 T7208 T7207 amod extreme,as
R1999 T7209 T7210 punct [,32
R2000 T7123 T7124 nummod 90,%
R2001 T7210 T7202 parataxis 32,was
R2002 T7211 T7210 punct ],32
R2003 T7212 T7183 punct .,reported
R2004 T7124 T7122 pobj %,to
R2005 T7214 T7215 nsubj Crosses,resulted
R2006 T7125 T7120 punct ),%
R2007 T7216 T7214 prep between,Crosses
R2008 T7217 T7218 nmod Capn2,animals
R2009 T7218 T7216 pobj animals,between
R2010 T7219 T7217 punct +,Capn2
R2011 T7220 T7217 punct /,Capn2
R2012 T7126 T7096 advmod essentially,was
R2013 T7221 T7217 punct +,Capn2
R2014 T7222 T7217 cc and,Capn2
R2015 T7223 T7217 conj Capn2,Capn2
R2016 T7224 T7223 punct +,Capn2
R2017 T7127 T7128 advmod the,same
R2018 T7128 T7096 acomp same,was
R2019 T7129 T7130 mark is,was
R2020 T7225 T7223 punct /,Capn2
R2021 T7130 T7128 advcl was,same
R2022 T7226 T7223 punct -,Capn2
R2023 T7227 T7215 advmod also,resulted
R2024 T7228 T7215 prep in,resulted
R2025 T7131 T7130 nsubj it,was
R2026 T7229 T7230 det a,proportion
R2027 T7230 T7228 pobj proportion,in
R2028 T7132 T7130 prep for,was
R2029 T7231 T7230 amod greater,proportion
R2030 T7232 T7233 mark than,expected
R2031 T7233 T7231 advcl expected,greater
R2032 T7133 T7134 det the,population
R2033 T7234 T7230 prep of,proportion
R2034 T7235 T7236 nmod Capn2,animals
R2035 T7134 T7132 pobj population,for
R2036 T7236 T7234 pobj animals,of
R2037 T7237 T7235 punct +,Capn2
R2038 T7238 T7235 punct /,Capn2
R2039 T7135 T7134 amod combined,population
R2040 T7239 T7235 punct -,Capn2
R2041 T7240 T7241 punct (,Table
R2042 T7241 T7215 parataxis Table,resulted
R2043 T7136 T7096 punct ", ",was
R2044 T7242 T7241 nummod 2,Table
R2045 T7243 T7241 punct ),Table
R2046 T7244 T7215 punct .,resulted
R2047 T7137 T7096 cc and,was
R2048 T7246 T7247 det An,degree
R2049 T7247 T7250 nsubjpass degree,seen
R2050 T7138 T7139 expl there,were
R2051 T7248 T7249 advmod even,higher
R2052 T7249 T7247 amod higher,degree
R2053 T7139 T7096 conj were,was
R2054 T7251 T7247 prep of,degree
R2055 T7252 T7253 nmod Capn2,over-representation
R2056 T7140 T7141 det an,average
R2057 T7253 T7251 pobj over-representation,of
R2058 T7254 T7252 punct +,Capn2
R2059 T7255 T7252 punct /,Capn2
R2060 T7141 T7139 attr average,were
R2061 T7256 T7252 punct -,Capn2
R2062 T7257 T7250 auxpass was,seen
R2063 T7258 T7259 advmod when,came
R2064 T7259 T7250 advcl came,seen
R2065 T7260 T7261 det the,allele
R2066 T7261 T7259 nsubj allele,came
R2067 T7142 T7141 prep of,average
R2068 T7262 T7261 compound mutant,allele
R2069 T7263 T7264 det the,mother
R2070 T7264 T7259 dobj mother,came
R2071 T7143 T7144 nummod six,pups
R2072 T7265 T7266 punct (,%
R2073 T7266 T7259 parataxis %,came
R2074 T7144 T7142 pobj pups,of
R2075 T7267 T7266 nummod 73,%
R2076 T7268 T7266 punct ),%
R2077 T7269 T7250 prep compared,seen
R2078 T7145 T7144 prep per,pups
R2079 T7270 T7269 prep to,compared
R2080 T7271 T7272 advmod when,came
R2081 T7146 T7145 pobj litter,per
R2082 T7272 T7270 pcomp came,to
R2083 T7273 T7272 nsubj it,came
R2084 T7147 T7141 punct ", ",average
R2085 T7148 T7149 dep which,is
R2086 T7274 T7272 prep from,came
R2087 T7149 T7141 relcl is,average
R2088 T7275 T7276 det the,father
R2089 T7276 T7274 pobj father,from
R2090 T7150 T7149 acomp normal,is
R2091 T7277 T7278 punct (,%
R2092 T7278 T7272 parataxis %,came
R2093 T7279 T7278 nummod 59,%
R2094 T7151 T7149 prep for,is
R2095 T7280 T7278 punct ),%
R2096 T7281 T7250 punct .,seen
R2097 T7152 T7153 det this,strain
R2098 T7283 T7284 prep In,compared
R2099 T7153 T7151 pobj strain,for
R2100 T7285 T7286 det these,crosses
R2101 T7286 T7283 pobj crosses,In
R2102 T7154 T7153 compound background,strain
R2103 T7287 T7288 det the,ratios
R2104 T7288 T7284 nsubj ratios,compared
R2105 T7289 T7288 prep of,ratios
R2106 T7155 T7139 punct .,were
R2107 T7290 T7289 pobj Capn2,of
R2108 T7291 T7290 punct +,Capn2
R2109 T7157 T7158 det A,proportion
R2110 T7292 T7290 punct /,Capn2
R2111 T7293 T7290 punct +,Capn2
R2112 T7294 T7288 prep to,ratios
R2113 T7295 T7296 nmod Capn2,animals
R2114 T7296 T7294 pobj animals,to
R2115 T7297 T7295 punct +,Capn2
R2116 T7298 T7295 punct /,Capn2
R2117 T7158 T7162 nsubj proportion,was
R2118 T7299 T7295 punct -,Capn2
R2119 T7300 T7288 prep among,ratios
R2120 T7301 T7300 pobj males,among
R2121 T7159 T7158 amod larger,proportion
R2122 T7302 T7301 cc or,males
R2123 T7303 T7301 conj females,males
R2124 T7304 T7284 advmod well,compared
R2125 T7160 T7161 mark than,expected
R2126 T7305 T7284 prep with,compared
R2127 T7306 T7307 det the,ratio
R2128 T7161 T7159 advcl expected,larger
R2129 T7163 T7164 nmod Capn2,animals
R2130 T7164 T7158 npadvmod animals,proportion
R2131 T7307 T7305 pobj ratio,with
R2132 T7165 T7163 punct +,Capn2
R2133 T7308 T7284 prep in,compared
R2134 T7309 T7310 det the,populations
R2135 T7310 T7308 pobj populations,in
R2136 T7166 T7163 punct /,Capn2
R2137 T7311 T7310 amod combined,populations
R2138 T7312 T7284 punct ;,compared
R2139 T7167 T7163 punct -,Capn2
R2140 T7313 T7314 nummod 77,%
R2141 T7314 T7315 nsubj %,were
R2142 T7315 T7284 conj were,compared
R2143 T7168 T7162 advmod also,was
R2144 T7316 T7314 prep of,%
R2145 T7317 T7316 pobj males,of
R2146 T7318 T7314 cc and,%
R2147 T7169 T7162 acomp evident,was
R2148 T7319 T7320 nummod 69,%
R2149 T7320 T7314 conj %,%
R2150 T7170 T7162 prep in,was
R2151 T7321 T7320 prep of,%
R2152 T7322 T7321 pobj females,of
R2153 T7323 T7315 attr Capn2,were
R2154 T7171 T7172 preconj both,embryos
R2155 T7324 T7323 punct +,Capn2
R2156 T7325 T7323 punct /,Capn2
R2157 T7326 T7323 punct -,Capn2
R2158 T7172 T7170 pobj embryos,in
R2159 T7327 T7328 advmod when,came
R2160 T7328 T7315 advcl came,were
R2161 T7329 T7330 det the,allele
R2162 T7173 T7172 nmod post-implantation,embryos
R2163 T7330 T7328 nsubj allele,came
R2164 T7331 T7330 compound mutant,allele
R2165 T7332 T7328 prep from,came
R2166 T7333 T7334 det the,mother
R2167 T7334 T7332 pobj mother,from
R2168 T7335 T7315 punct ", ",were
R2169 T7174 T7173 cc and,post-implantation
R2170 T7336 T7315 cc and,were
R2171 T7337 T7338 nummod 62,%
R2172 T7338 T7339 nsubj %,were
R2173 T7175 T7173 conj pre-implantation,post-implantation
R2174 T7339 T7315 conj were,were
R2175 T7340 T7338 prep of,%
R2176 T7341 T7340 pobj males,of
R2177 T7176 T7177 punct (,Table
R2178 T7342 T7338 cc and,%
R2179 T7343 T7344 nummod 55,%
R2180 T7344 T7338 conj %,%
R2181 T7177 T7162 parataxis Table,was
R2182 T7345 T7344 prep of,%
R2183 T7346 T7345 pobj females,of
R2184 T7347 T7339 attr Capn2,were
R2185 T7178 T7177 nummod 2,Table
R2186 T7348 T7347 punct +,Capn2
R2187 T7349 T7347 punct /,Capn2
R2188 T7350 T7347 punct -,Capn2
R2189 T7179 T7177 punct ),Table
R2190 T7351 T7352 advmod when,came
R2191 T7352 T7339 advcl came,were
R2192 T7180 T7162 punct .,was
R2193 T7353 T7354 det the,allele
R2194 T7354 T7352 nsubj allele,came
R2195 T7355 T7354 compound mutant,allele
R2196 T7182 T7183 advmod Interestingly,reported
R2197 T7356 T7352 prep from,came
R2198 T7184 T7183 punct ", ",reported
R2199 T7357 T7358 det the,father
R2200 T7358 T7356 pobj father,from
R2201 T7185 T7186 det a,over-representation
R2202 T7359 T7339 punct .,were
R2203 T7186 T7183 nsubjpass over-representation,reported
R2204 T7187 T7186 amod similar,over-representation
R2205 T7188 T7186 prep of,over-representation
R2206 T7189 T7190 amod heterozygous,animals
R2207 T7190 T7188 pobj animals,of
R2208 T7191 T7183 auxpass was,reported
R2209 T7192 T7183 advmod also,reported
R2210 T7193 T7183 prep in,reported
R2211 T7194 T7193 pobj one,in
R2212 T7195 T7194 prep of,one
R2213 T7196 T7197 det the,lines
R2214 T7197 T7195 pobj lines,of
R2215 T7198 T7197 nmod Capn4,lines
R2216 T7199 T7197 amod transgenic,lines
R2217 T7200 T7183 punct ", ",reported
R2218 T7201 T7202 mark though,was
R6470 T21857 T21858 amod Nested,strategy
R6471 T21859 T21858 compound PCR,strategy
R6472 T21860 T21858 prep for,strategy
R6473 T21861 T21860 pobj genotyping,for
R6474 T21862 T21861 prep of,genotyping
R6475 T21863 T21864 amod pre-implantation,embryos
R6476 T21864 T21862 pobj embryos,of
R6477 T21865 T21858 punct .,strategy
R6478 T21867 T21868 prep Due,developed
R6479 T21869 T21867 pcomp to,Due
R6480 T21870 T21871 det the,scarcity
R6481 T21871 T21867 pobj scarcity,Due
R6482 T21872 T21871 prep of,scarcity
R6483 T21873 T21874 amod extractable,material
R6484 T21874 T21872 pobj material,of
R6485 T21875 T21874 amod genetic,material
R6486 T21876 T21868 punct ", ",developed
R6487 T21877 T21878 det a,strategy
R6488 T21878 T21868 nsubjpass strategy,developed
R6489 T21879 T21878 amod nested,strategy
R6490 T21880 T21878 compound PCR,strategy
R6491 T21881 T21868 auxpass was,developed
R6492 T21882 T21868 prep in,developed
R6493 T21883 T21882 pobj order,in
R6494 T21884 T21885 aux to,genotype
R6495 T21885 T21883 acl genotype,order
R6496 T21886 T21887 amod pre-implantation,embryos
R6497 T21887 T21885 dobj embryos,genotype
R6498 T21888 T21868 punct .,developed
R6499 T21890 T21891 amod Separate,reactions
R6500 T21891 T21892 nsubjpass reactions,used
R6501 T21893 T21892 auxpass were,used
R6502 T21894 T21895 aux to,amplify
R6503 T21895 T21892 advcl amplify,used
R6504 T21896 T21897 det a,fragment
R6505 T21897 T21895 dobj fragment,amplify
R6506 T21898 T21899 nummod 429,bp
R6507 T21899 T21897 compound bp,fragment
R6508 T21900 T21897 prep from,fragment
R6509 T21901 T21902 det the,allele
R6510 T21902 T21900 pobj allele,from
R6511 T21903 T21904 amod wild,type
R6512 T21904 T21902 compound type,allele
R6513 T21905 T21904 punct -,type
R6514 T21906 T21897 cc and,fragment
R6515 T21907 T21908 det a,segment
R6516 T21908 T21897 conj segment,fragment
R6517 T21909 T21910 nummod 389,bp
R6518 T21910 T21908 compound bp,segment
R6519 T21911 T21908 prep from,segment
R6520 T21912 T21913 det the,allele
R6521 T21913 T21911 pobj allele,from
R6522 T21914 T21913 compound mutant,allele
R6523 T21915 T21897 punct ", ",fragment
R6524 T21916 T21897 appos both,fragment
R6525 T21917 T21916 acl spanning,both
R6526 T21918 T21919 det the,end
R6527 T21919 T21917 dobj end,spanning
R6528 T21920 T21919 nummod 3,end
R6529 T21921 T21920 punct ',3
R6530 T21922 T21919 prep of,end
R6531 T21923 T21924 det the,arm
R6532 T21924 T21922 pobj arm,of
R6533 T21925 T21924 amod short,arm
R6534 T21926 T21927 punct (,upstream
R6535 T21927 T21924 parataxis upstream,arm
R6536 T21928 T21927 punct ),upstream
R6537 T21929 T21924 prep of,arm
R6538 T21930 T21931 det the,vector
R6539 T21931 T21929 pobj vector,of
R6540 T21932 T21931 compound targeting,vector
R6541 T21933 T21892 punct .,used
R6542 T21935 T21936 prep In,co-amplified
R6543 T21937 T21938 det both,reactions
R6544 T21938 T21935 pobj reactions,In
R6545 T21939 T21936 punct ", ",co-amplified
R6546 T21940 T21941 det a,sequence
R6547 T21941 T21936 nsubjpass sequence,co-amplified
R6548 T21942 T21943 nummod 213,bp
R6549 T21943 T21941 compound bp,sequence
R6550 T21944 T21941 acl located,sequence
R6551 T21945 T21944 prep within,located
R6552 T21946 T21947 det the,arm
R6553 T21947 T21945 pobj arm,within
R6554 T21948 T21947 amod short,arm
R6555 T21949 T21936 auxpass was,co-amplified
R6556 T21950 T21936 prep with,co-amplified
R6557 T21951 T21952 det the,products
R6558 T21952 T21950 pobj products,with
R6559 T21953 T21952 punct ',products
R6560 T21954 T21952 amod diagnostic,products
R6561 T21955 T21952 punct ',products
R6562 T21956 T21936 prep as,co-amplified
R6563 T21957 T21958 det an,control
R6564 T21958 T21956 pobj control,as
R6565 T21959 T21958 amod internal,control
R6566 T21960 T21936 punct .,co-amplified
R6567 T21962 T21963 det The,primers
R6568 T21963 T21966 nsubjpass primers,used
R6569 T21964 T21963 amod same,primers
R6570 T21965 T21963 compound sense,primers
R6571 T21967 T21966 auxpass were,used
R6572 T21968 T21969 aux to,amplify
R6573 T21969 T21966 advcl amplify,used
R6574 T21970 T21969 punct ',amplify
R6575 T21971 T21972 amod diagnostic,sequences
R6576 T21972 T21969 dobj sequences,amplify
R6577 T21973 T21972 punct ',sequences
R6578 T21974 T21972 prep in,sequences
R6579 T21975 T21976 det both,reactions
R6580 T21976 T21974 pobj reactions,in
R6581 T21977 T21969 punct ", ",amplify
R6582 T21978 T21979 mark whereas,were
R6583 T21979 T21969 advcl were,amplify
R6584 T21980 T21981 det the,primers
R6585 T21981 T21979 nsubj primers,were
R6586 T21982 T21981 compound antisense,primers
R6587 T21983 T21984 npadvmod allele,specific
R6588 T21984 T21979 acomp specific,were
R6589 T21985 T21984 punct -,specific
R6590 T21986 T21966 punct .,used
R6591 T21988 T21989 det The,primers
R6592 T21989 T21990 nsubjpass primers,depicted
R6593 T21991 T21989 punct ", ",primers
R6594 T21992 T21989 acl represented,primers
R6595 T21993 T21992 agent by,represented
R6596 T21994 T21993 pobj triangles,by
R6597 T21995 T21990 punct ", ",depicted
R6598 T21996 T21990 auxpass are,depicted
R6599 T21997 T21990 prep in,depicted
R6600 T21998 T21999 nummod two,sets
R6601 T21999 T21997 pobj sets,in
R6602 T22000 T21999 punct (,sets
R6603 T22001 T21999 amod nested,sets
R6604 T22002 T21999 punct ),sets
R6605 T22003 T21999 prep for,sets
R6606 T22004 T22003 pobj each,for
R6607 T22005 T22004 prep of,each
R6608 T22006 T22007 det the,reactions
R6609 T22007 T22005 pobj reactions,of
R6610 T22008 T22007 nummod three,reactions
R6611 T22009 T21990 punct .,depicted
R6612 T22011 T22012 nsubjpass Exons,represented
R6613 T22012 T22014 ccomp represented,cassette
R6614 T22013 T22012 auxpass are,represented
R6615 T22015 T22012 agent by,represented
R6616 T22016 T22017 amod open,rectangles
R6617 T22017 T22015 pobj rectangles,by
R6618 T22018 T22017 punct ", ",rectangles
R6619 T22019 T22017 amod vertical,rectangles
R6620 T22020 T22014 punct ", ",cassette
R6621 T22021 T22014 det the,cassette
R6622 T22022 T22023 compound PGK,Neo
R6623 T22023 T22014 compound Neo,cassette
R6624 T22024 T22023 punct -,Neo
R6625 T22025 T22014 agent by,cassette
R6626 T22026 T22027 det an,rectangle
R6627 T22027 T22025 pobj rectangle,by
R6628 T22028 T22027 amod open,rectangle
R6629 T22029 T22027 punct ", ",rectangle
R6630 T22030 T22027 amod horizontal,rectangle
R6631 T22031 T22014 punct ", ",cassette
R6632 T22032 T22033 mark while,denote
R6633 T22033 T22014 advcl denote,cassette
R6634 T22034 T22035 amod thin,lines
R6635 T22035 T22033 nsubj lines,denote
R6636 T22036 T22035 amod vertical,lines
R6637 T22037 T22038 det the,boundaries
R6638 T22038 T22033 dobj boundaries,denote
R6639 T22039 T22038 prep of,boundaries
R6640 T22040 T22041 det the,arm
R6641 T22041 T22039 pobj arm,of
R6642 T22042 T22041 amod short,arm
R6643 T22043 T22038 cc and,boundaries
R6644 T22044 T22045 det the,end
R6645 T22045 T22038 conj end,boundaries
R6646 T22046 T22045 nummod 5,end
R6647 T22047 T22046 punct ',5
R6648 T22048 T22045 prep of,end
R6649 T22049 T22050 det the,arm
R6650 T22050 T22048 pobj arm,of
R6651 T22051 T22050 amod long,arm
R6652 T22052 T22050 punct (,arm
R6653 T22053 T22050 amod downstream,arm
R6654 T22054 T22050 punct ),arm
R6655 T22055 T22014 punct .,cassette
R6656 T22057 T22058 det A,rectangle
R6657 T22058 T22062 nsubj rectangle,delineates
R6658 T22059 T22058 amod grey,rectangle
R6659 T22060 T22058 punct ", ",rectangle
R6660 T22061 T22058 amod horizontal,rectangle
R6661 T22063 T22064 det the,segment
R6662 T22064 T22062 dobj segment,delineates
R6663 T22065 T22064 prep of,segment
R6664 T22066 T22067 det the,allele
R6665 T22067 T22065 pobj allele,of
R6666 T22068 T22069 amod wild,type
R6667 T22069 T22067 compound type,allele
R6668 T22070 T22069 punct -,type
R6669 T22071 T22072 dep that,replaced
R6670 T22072 T22064 relcl replaced,segment
R6671 T22073 T22072 auxpass is,replaced
R6672 T22074 T22072 agent by,replaced
R6673 T22075 T22076 det the,cassette
R6674 T22076 T22074 pobj cassette,by
R6675 T22077 T22078 compound PGK,Neo
R6676 T22078 T22076 compound Neo,cassette
R6677 T22079 T22078 punct -,Neo
R6678 T22080 T22072 prep in,replaced
R6679 T22081 T22082 det the,allele
R6680 T22082 T22080 pobj allele,in
R6681 T22083 T22082 compound mutant,allele
R6682 T22084 T22062 punct .,delineates
R6683 T22641 T22640 prep of,Genotyping
R6684 T22642 T22643 amod pre-implantation,embryos
R6685 T22643 T22641 pobj embryos,of
R6686 T22644 T22640 punct .,Genotyping
R6687 T22646 T22647 det A,strategy
R6688 T22647 T22650 nsubjpass strategy,used
R6689 T22648 T22647 amod nested,strategy
R6690 T22649 T22647 compound PCR,strategy
R6691 T22651 T22650 auxpass was,used
R6692 T22652 T22653 aux to,genotype
R6693 T22653 T22650 advcl genotype,used
R6694 T22654 T22653 dobj embryos,genotype
R6695 T22655 T22656 amod prior,to
R6696 T22656 T22653 prep to,genotype
R6697 T22657 T22656 pobj implantation,to
R6698 T22658 T22659 mark as,described
R6699 T22659 T22653 advcl described,genotype
R6700 T22660 T22659 prep in,described
R6701 T22661 T22660 pobj Figure,in
R6702 T22662 T22661 nummod 4,Figure
R6703 T22663 T22650 punct .,used
R6704 T22665 T22666 nmod Capn2,mice
R6705 T22666 T22670 nsubjpass mice,mated
R6706 T22667 T22665 punct +,Capn2
R6707 T22668 T22665 punct /,Capn2
R6708 T22669 T22665 punct -,Capn2
R6709 T22671 T22670 auxpass were,mated
R6710 T22672 T22670 cc and,mated
R6711 T22673 T22674 det the,date
R6712 T22674 T22675 nsubj date,established
R6713 T22675 T22670 conj established,mated
R6714 T22676 T22674 prep of,date
R6715 T22677 T22676 pobj fertilization,of
R6716 T22678 T22675 agent by,established
R6717 T22679 T22680 det the,appearance
R6718 T22680 T22678 pobj appearance,by
R6719 T22681 T22680 prep of,appearance
R6720 T22682 T22683 amod vaginal,plugs
R6721 T22683 T22681 pobj plugs,of
R6722 T22684 T22675 punct .,established
R6723 T22686 T22687 nmod Blastocyst,embryos
R6724 T22687 T22695 nsubjpass embryos,flushed
R6725 T22688 T22689 punct (,E3.5
R6726 T22689 T22686 parataxis E3.5,Blastocyst
R6727 T22690 T22689 punct ),E3.5
R6728 T22691 T22686 cc or,Blastocyst
R6729 T22692 T22693 nummod 8,cell
R6730 T22693 T22686 conj cell,Blastocyst
R6731 T22694 T22693 punct -,cell
R6732 T22696 T22697 punct (,E2.5
R6733 T22697 T22687 parataxis E2.5,embryos
R6734 T22698 T22697 punct ),E2.5
R6735 T22699 T22695 auxpass were,flushed
R6736 T22700 T22695 prep from,flushed
R6737 T22701 T22702 det the,oviducts
R6738 T22702 T22700 pobj oviducts,from
R6739 T22703 T22695 cc and,flushed
R6740 T22704 T22705 advmod then,digested
R6741 T22705 T22695 conj digested,flushed
R6742 T22706 T22705 prep with,digested
R6743 T22707 T22708 compound proteinase,K
R6744 T22708 T22706 pobj K,with
R6745 T22709 T22695 punct .,flushed
R6746 T22711 T22712 prep In,co-amplified
R6747 T22713 T22714 amod separate,segments
R6748 T22714 T22711 pobj segments,In
R6749 T22715 T22714 compound reactions,segments
R6750 T22716 T22714 acl found,segments
R6751 T22717 T22716 advmod exclusively,found
R6752 T22718 T22716 prep in,found
R6753 T22719 T22720 preconj either,type
R6754 T22720 T22718 pobj type,in
R6755 T22721 T22720 det the,type
R6756 T22722 T22720 amod wild,type
R6757 T22723 T22720 punct -,type
R6758 T22724 T22720 cc or,type
R6759 T22725 T22720 conj mutant,type
R6760 T22726 T22712 nsubjpass alleles,co-amplified
R6761 T22727 T22712 auxpass were,co-amplified
R6762 T22728 T22712 prep with,co-amplified
R6763 T22729 T22730 det an,sequence
R6764 T22730 T22728 pobj sequence,with
R6765 T22731 T22730 amod internal,sequence
R6766 T22732 T22730 compound control,sequence
R6767 T22733 T22730 punct ", ",sequence
R6768 T22734 T22730 acl located,sequence
R6769 T22735 T22734 prep in,located
R6770 T22736 T22737 det the,arm
R6771 T22737 T22735 pobj arm,in
R6772 T22738 T22737 amod short,arm
R6773 T22739 T22737 punct (,arm
R6774 T22740 T22737 amod upstream,arm
R6775 T22741 T22737 punct ),arm
R6776 T22742 T22737 prep of,arm
R6777 T22743 T22744 det the,vector
R6778 T22744 T22742 pobj vector,of
R6779 T22745 T22744 compound targeting,vector
R6780 T22746 T22744 punct ", ",vector
R6781 T22747 T22748 dep which,found
R6782 T22748 T22744 relcl found,vector
R6783 T22749 T22748 auxpass is,found
R6784 T22750 T22748 prep in,found
R6785 T22751 T22752 det both,alleles
R6786 T22752 T22750 pobj alleles,in
R6787 T22753 T22712 punct .,co-amplified
R6788 T22755 T22756 det The,products
R6789 T22756 T22758 nsubj products,were
R6790 T22757 T22756 amod final,products
R6791 T22759 T22760 nummod 429,bp
R6792 T22760 T22758 attr bp,were
R6793 T22761 T22758 prep for,were
R6794 T22762 T22763 det the,allele
R6795 T22763 T22761 pobj allele,for
R6796 T22764 T22765 amod wild,type
R6797 T22765 T22763 compound type,allele
R6798 T22766 T22765 punct -,type
R6799 T22767 T22758 punct ", ",were
R6800 T22768 T22769 nummod 389,bp
R6801 T22769 T22758 conj bp,were
R6802 T22770 T22769 prep for,bp
R6803 T22771 T22772 det the,allele
R6804 T22772 T22770 pobj allele,for
R6805 T22773 T22772 compound mutant,allele
R6806 T22774 T22769 punct ", ",bp
R6807 T22775 T22769 cc and,bp
R6808 T22776 T22777 nummod 213,bp
R6809 T22777 T22769 conj bp,bp
R6810 T22778 T22777 prep for,bp
R6811 T22779 T22780 det the,control
R6812 T22780 T22778 pobj control,for
R6813 T22781 T22780 amod internal,control
R6814 T22782 T22758 punct .,were
R6815 T22784 T22785 punct (,A
R6816 T22785 T22786 meta A,example
R6817 T22787 T22785 punct ),A
R6818 T22788 T22786 det A,example
R6819 T22789 T22786 amod representative,example
R6820 T22790 T22786 prep of,example
R6821 T22791 T22792 det the,genotyping
R6822 T22792 T22790 pobj genotyping,of
R6823 T22793 T22792 prep of,genotyping
R6824 T22794 T22795 compound blastocyst,embryos
R6825 T22795 T22793 pobj embryos,of
R6826 T22796 T22795 compound stage,embryos
R6827 T22797 T22786 punct .,example
R6828 T22799 T22800 nmod Embryos,1
R6829 T22800 T22802 nsubj 1,were
R6830 T22801 T22800 punct #,1
R6831 T22803 T22800 punct ", ",1
R6832 T22804 T22800 conj 2,1
R6833 T22805 T22804 punct ", ",2
R6834 T22806 T22804 conj 4,2
R6835 T22807 T22806 punct ", ",4
R6836 T22808 T22806 conj 5,4
R6837 T22809 T22808 punct ", ",5
R6838 T22810 T22808 cc and,5
R6839 T22811 T22808 conj 6,5
R6840 T22812 T22802 attr Capn2,were
R6841 T22813 T22812 punct +,Capn2
R6842 T22814 T22812 punct /,Capn2
R6843 T22815 T22812 punct -,Capn2
R6844 T22816 T22817 mark whereas,were
R6845 T22817 T22802 advcl were,were
R6846 T22818 T22819 nmod embryos,3
R6847 T22819 T22817 nsubj 3,were
R6848 T22820 T22819 punct #,3
R6849 T22821 T22819 cc and,3
R6850 T22822 T22823 punct #,7
R6851 T22823 T22819 conj 7,3
R6852 T22824 T22817 attr Capn2,were
R6853 T22825 T22824 punct +,Capn2
R6854 T22826 T22824 punct /,Capn2
R6855 T22827 T22824 punct +,Capn2
R6856 T22828 T22817 punct ", ",were
R6857 T22829 T22817 advcl denoted,were
R6858 T22830 T22829 agent by,denoted
R6859 T22831 T22832 det the,absence
R6860 T22832 T22830 pobj absence,by
R6861 T22833 T22832 prep of,absence
R6862 T22834 T22835 det the,signal
R6863 T22835 T22833 pobj signal,of
R6864 T22836 T22837 nummod 389,bp
R6865 T22837 T22835 compound bp,signal
R6866 T22838 T22835 compound mutant,signal
R6867 T22839 T22802 punct .,were
R6868 T22841 T22842 punct (,B
R6869 T22842 T22843 meta B,shown
R6870 T22844 T22842 punct ),B
R6871 T22845 T22846 det An,example
R6872 T22846 T22843 nsubjpass example,shown
R6873 T22847 T22846 prep of,example
R6874 T22848 T22849 det the,genotyping
R6875 T22849 T22847 pobj genotyping,of
R6876 T22850 T22849 prep of,genotyping
R6877 T22851 T22852 nummod 8,cell
R6878 T22852 T22854 compound cell,embryos
R6879 T22853 T22852 punct -,cell
R6880 T22854 T22850 pobj embryos,of
R6881 T22855 T22843 auxpass is,shown
R6882 T22856 T22843 punct .,shown
R6883 T22858 T22859 nmod Embryos,1
R6884 T22859 T22861 nsubj 1,were
R6885 T22860 T22859 punct #,1
R6886 T22862 T22859 punct ", ",1
R6887 T22863 T22859 conj 2,1
R6888 T22864 T22863 punct ", ",2
R6889 T22865 T22863 conj 3,2
R6890 T22866 T22865 punct ", ",3
R6891 T22867 T22865 conj 5,3
R6892 T22868 T22867 punct ", ",5
R6893 T22869 T22867 cc and,5
R6894 T22870 T22867 conj 6,5
R6895 T22871 T22861 attr Capn2,were
R6896 T22872 T22871 punct +,Capn2
R6897 T22873 T22871 punct /,Capn2
R6898 T22874 T22871 punct -,Capn2
R6899 T22875 T22876 mark while,was
R6900 T22876 T22861 advcl was,were
R6901 T22877 T22876 nsubj embryo,was
R6902 T22878 T22877 punct #,embryo
R6903 T22879 T22877 nummod 4,embryo
R6904 T22880 T22876 attr Capn2,was
R6905 T22881 T22880 punct -,Capn2
R6906 T22882 T22880 punct /,Capn2
R6907 T22883 T22880 punct -,Capn2
R6908 T22884 T22880 punct ", ",Capn2
R6909 T22885 T22880 acl marked,Capn2
R6910 T22886 T22885 agent by,marked
R6911 T22887 T22888 det the,absence
R6912 T22888 T22886 pobj absence,by
R6913 T22889 T22888 prep of,absence
R6914 T22890 T22891 det the,signal
R6915 T22891 T22889 pobj signal,of
R6916 T22892 T22893 nummod 429,bp
R6917 T22893 T22891 nmod bp,signal
R6918 T22894 T22895 amod wild,type
R6919 T22895 T22891 compound type,signal
R6920 T22896 T22895 punct -,type
R6921 T22897 T22861 punct .,were
R6922 T22899 T22900 punct (,M
R6923 T22900 T22901 nsubj M,denotes
R6924 T22902 T22900 punct ),M
R6925 T22903 T22904 det the,marker
R6926 T22904 T22901 dobj marker,denotes
R6927 T22905 T22906 amod molecular,weight
R6928 T22906 T22904 compound weight,marker
R6929 T22907 T22901 punct .,denotes
R6930 T22936 T22937 compound Genotype,distribution
R6931 T22938 T22937 prep of,distribution
R6932 T22939 T22938 pobj offspring,of
R6933 T22940 T22939 acl derived,offspring
R6934 T22941 T22940 prep from,derived
R6935 T22942 T22943 nmod Capn2,mice
R6936 T22943 T22941 pobj mice,from
R6937 T22944 T22943 amod transgenic,mice
R6938 T22945 T22937 punct .,distribution