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PMC:1382200 / 8230-9523 JSONTXT 4 Projects

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Id Subject Object Predicate Lexical cue
T21151 10-26 NN denotes Characterization
T21152 27-29 IN denotes of
T21153 30-38 VBN denotes targeted
T21155 39-41 NN denotes ES
T21156 42-46 NN denotes cell
T21154 47-52 NNS denotes lines
T21157 52-53 . denotes .
T21158 53-141 sentence denotes (A) Targeted disruption of the Capn2 locus was detected initially by Southern blotting.
T21159 54-55 -LRB- denotes (
T21160 55-56 LS denotes A
T21162 56-57 -RRB- denotes )
T21163 58-66 VBN denotes Targeted
T21164 67-77 NN denotes disruption
T21165 78-80 IN denotes of
T21166 81-84 DT denotes the
T21168 85-90 NN denotes Capn2
T21167 91-96 NN denotes locus
T21169 97-100 VBD denotes was
T21161 101-109 VBN denotes detected
T21170 110-119 RB denotes initially
T21171 120-122 IN denotes by
T21172 123-131 NNP denotes Southern
T21173 132-140 NN denotes blotting
T21174 140-141 . denotes .
T21175 141-366 sentence denotes Membranes were blotted with BamHI-digested genomic DNA extracted from ES cells and hybridized with a DIG-labeled 823 bp BamHI/HindIII fragment located immediately upstream of the short arm of the targeting vector (Figure 1).
T21176 142-151 NNS denotes Membranes
T21178 152-156 VBD denotes were
T21177 157-164 VBN denotes blotted
T21179 165-169 IN denotes with
T21180 170-175 NN denotes BamHI
T21182 175-176 HYPH denotes -
T21181 176-184 VBN denotes digested
T21184 185-192 JJ denotes genomic
T21183 193-196 NN denotes DNA
T21185 197-206 VBN denotes extracted
T21186 207-211 IN denotes from
T21187 212-214 NN denotes ES
T21188 215-220 NNS denotes cells
T21189 221-224 CC denotes and
T21190 225-235 VBD denotes hybridized
T21191 236-240 IN denotes with
T21192 241-242 DT denotes a
T21194 243-246 NN denotes DIG
T21196 246-247 HYPH denotes -
T21195 247-254 VBN denotes labeled
T21197 255-258 CD denotes 823
T21198 259-261 NN denotes bp
T21199 262-267 NN denotes BamHI
T21201 267-268 HYPH denotes /
T21200 268-275 NN denotes HindIII
T21193 276-284 NN denotes fragment
T21202 285-292 VBN denotes located
T21203 293-304 RB denotes immediately
T21204 305-313 IN denotes upstream
T21205 314-316 IN denotes of
T21206 317-320 DT denotes the
T21208 321-326 JJ denotes short
T21207 327-330 NN denotes arm
T21209 331-333 IN denotes of
T21210 334-337 DT denotes the
T21212 338-347 NN denotes targeting
T21211 348-354 NN denotes vector
T21213 355-356 -LRB- denotes (
T21214 356-362 NN denotes Figure
T21215 363-364 CD denotes 1
T21216 364-365 -RRB- denotes )
T21217 365-366 . denotes .
T21218 366-570 sentence denotes A 3.5-kb BamHI fragment corresponding to the wild-type allele was present in all cells, whereas a 5.3-kb fragment from the mutant allele was detected in two targeted cell lines, designated ES27 and ES36.
T21219 367-368 DT denotes A
T21221 369-372 CD denotes 3.5
T21223 372-373 HYPH denotes -
T21222 373-375 NN denotes kb
T21224 376-381 NN denotes BamHI
T21220 382-390 NN denotes fragment
T21226 391-404 VBG denotes corresponding
T21227 405-407 IN denotes to
T21228 408-411 DT denotes the
T21230 412-416 JJ denotes wild
T21232 416-417 HYPH denotes -
T21231 417-421 NN denotes type
T21229 422-428 NN denotes allele
T21225 429-432 VBD denotes was
T21233 433-440 JJ denotes present
T21234 441-443 IN denotes in
T21235 444-447 DT denotes all
T21236 448-453 NNS denotes cells
T21237 453-455 , denotes ,
T21238 455-462 IN denotes whereas
T21240 463-464 DT denotes a
T21242 465-468 CD denotes 5.3
T21244 468-469 HYPH denotes -
T21243 469-471 NN denotes kb
T21241 472-480 NN denotes fragment
T21245 481-485 IN denotes from
T21246 486-489 DT denotes the
T21248 490-496 NN denotes mutant
T21247 497-503 NN denotes allele
T21249 504-507 VBD denotes was
T21239 508-516 VBN denotes detected
T21250 517-519 IN denotes in
T21251 520-523 CD denotes two
T21253 524-532 VBN denotes targeted
T21254 533-537 NN denotes cell
T21252 538-543 NNS denotes lines
T21255 543-545 , denotes ,
T21256 545-555 VBN denotes designated
T21257 556-560 NN denotes ES27
T21258 561-564 CC denotes and
T21259 565-569 NN denotes ES36
T21260 569-570 . denotes .
T21261 570-751 sentence denotes (B) PCR genotyping was carried out with two separate reactions designed to amplify either a 2,748 bp segment from the wild-type allele or a 2,711 bp segment from the mutant allele.
T21262 571-572 -LRB- denotes (
T21263 572-573 LS denotes B
T21265 573-574 -RRB- denotes )
T21266 575-578 NN denotes PCR
T21267 579-589 NN denotes genotyping
T21268 590-593 VBD denotes was
T21264 594-601 VBN denotes carried
T21269 602-605 RP denotes out
T21270 606-610 IN denotes with
T21271 611-614 CD denotes two
T21273 615-623 JJ denotes separate
T21272 624-633 NNS denotes reactions
T21274 634-642 VBN denotes designed
T21275 643-645 TO denotes to
T21276 646-653 VB denotes amplify
T21277 654-660 CC denotes either
T21279 661-662 DT denotes a
T21280 663-668 CD denotes 2,748
T21281 669-671 NN denotes bp
T21278 672-679 NN denotes segment
T21282 680-684 IN denotes from
T21283 685-688 DT denotes the
T21285 689-693 JJ denotes wild
T21287 693-694 HYPH denotes -
T21286 694-698 NN denotes type
T21284 699-705 NN denotes allele
T21288 706-708 CC denotes or
T21289 709-710 DT denotes a
T21291 711-716 CD denotes 2,711
T21292 717-719 NN denotes bp
T21290 720-727 NN denotes segment
T21293 728-732 IN denotes from
T21294 733-736 DT denotes the
T21296 737-743 NN denotes mutant
T21295 744-750 NN denotes allele
T21297 750-751 . denotes .
T21298 751-909 sentence denotes Both reactions used a common sense primer located in intron 4, outside the short arm of the targeting vector, and distinct allele-specific antisense primers.
T21299 752-756 DT denotes Both
T21300 757-766 NNS denotes reactions
T21301 767-771 VBD denotes used
T21302 772-773 DT denotes a
T21304 774-780 JJ denotes common
T21305 781-786 NN denotes sense
T21303 787-793 NN denotes primer
T21306 794-801 VBN denotes located
T21307 802-804 IN denotes in
T21308 805-811 NN denotes intron
T21309 812-813 CD denotes 4
T21310 813-815 , denotes ,
T21311 815-822 IN denotes outside
T21312 823-826 DT denotes the
T21314 827-832 JJ denotes short
T21313 833-836 NN denotes arm
T21315 837-839 IN denotes of
T21316 840-843 DT denotes the
T21318 844-853 NN denotes targeting
T21317 854-860 NN denotes vector
T21319 860-862 , denotes ,
T21320 862-865 CC denotes and
T21321 866-874 JJ denotes distinct
T21323 875-881 NN denotes allele
T21325 881-882 HYPH denotes -
T21324 882-890 JJ denotes specific
T21326 891-900 NN denotes antisense
T21322 901-908 NNS denotes primers
T21327 908-909 . denotes .
T21328 909-1102 sentence denotes The reaction to detect the wild-type allele used an antisense primer located in exon 7 while the amplification of the mutant sequence was done with an antisense primer in the PGK-Neo cassette.
T21329 910-913 DT denotes The
T21330 914-922 NN denotes reaction
T21332 923-925 TO denotes to
T21333 926-932 VB denotes detect
T21334 933-936 DT denotes the
T21336 937-941 JJ denotes wild
T21338 941-942 HYPH denotes -
T21337 942-946 NN denotes type
T21335 947-953 NN denotes allele
T21331 954-958 VBD denotes used
T21339 959-961 DT denotes an
T21341 962-971 NN denotes antisense
T21340 972-978 NN denotes primer
T21342 979-986 VBN denotes located
T21343 987-989 IN denotes in
T21344 990-994 NN denotes exon
T21345 995-996 CD denotes 7
T21346 997-1002 IN denotes while
T21348 1003-1006 DT denotes the
T21349 1007-1020 NN denotes amplification
T21350 1021-1023 IN denotes of
T21351 1024-1027 DT denotes the
T21353 1028-1034 NN denotes mutant
T21352 1035-1043 NN denotes sequence
T21354 1044-1047 VBD denotes was
T21347 1048-1052 VBN denotes done
T21355 1053-1057 IN denotes with
T21356 1058-1060 DT denotes an
T21358 1061-1070 NN denotes antisense
T21357 1071-1077 NN denotes primer
T21359 1078-1080 IN denotes in
T21360 1081-1084 DT denotes the
T21362 1085-1088 NN denotes PGK
T21364 1088-1089 HYPH denotes -
T21363 1089-1092 NN denotes Neo
T21361 1093-1101 NN denotes cassette
T21365 1101-1102 . denotes .
T21366 1102-1252 sentence denotes The results confirm the presence of the wild-type allele in all cells, whereas the mutant allele signal was observed only in the two targeted clones.
T21367 1103-1106 DT denotes The
T21368 1107-1114 NNS denotes results
T21369 1115-1122 VBP denotes confirm
T21370 1123-1126 DT denotes the
T21371 1127-1135 NN denotes presence
T21372 1136-1138 IN denotes of
T21373 1139-1142 DT denotes the
T21375 1143-1147 JJ denotes wild
T21377 1147-1148 HYPH denotes -
T21376 1148-1152 NN denotes type
T21374 1153-1159 NN denotes allele
T21378 1160-1162 IN denotes in
T21379 1163-1166 DT denotes all
T21380 1167-1172 NNS denotes cells
T21381 1172-1174 , denotes ,
T21382 1174-1181 IN denotes whereas
T21384 1182-1185 DT denotes the
T21386 1186-1192 NN denotes mutant
T21387 1193-1199 NN denotes allele
T21385 1200-1206 NN denotes signal
T21388 1207-1210 VBD denotes was
T21383 1211-1219 VBN denotes observed
T21389 1220-1224 RB denotes only
T21390 1225-1227 IN denotes in
T21391 1228-1231 DT denotes the
T21393 1232-1235 CD denotes two
T21394 1236-1244 VBN denotes targeted
T21392 1245-1251 NNS denotes clones
T21395 1251-1252 . denotes .
T21396 1252-1293 sentence denotes (M) denotes the molecular weight marker.
T21397 1253-1254 -LRB- denotes (
T21399 1254-1255 NN denotes M
T21400 1255-1256 -RRB- denotes )
T21398 1257-1264 VBZ denotes denotes
T21401 1265-1268 DT denotes the
T21403 1269-1278 JJ denotes molecular
T21404 1279-1285 NN denotes weight
T21402 1286-1292 NN denotes marker
T21405 1292-1293 . denotes .
R6232 T21152 T21151 prep of,Characterization
R6233 T21153 T21154 amod targeted,lines
R6234 T21154 T21152 pobj lines,of
R6235 T21155 T21156 compound ES,cell
R6236 T21156 T21154 compound cell,lines
R6237 T21157 T21151 punct .,Characterization
R6238 T21159 T21160 punct (,A
R6239 T21160 T21161 meta A,detected
R6240 T21162 T21160 punct ),A
R6241 T21163 T21164 amod Targeted,disruption
R6242 T21164 T21161 nsubjpass disruption,detected
R6243 T21165 T21164 prep of,disruption
R6244 T21166 T21167 det the,locus
R6245 T21167 T21165 pobj locus,of
R6246 T21168 T21167 compound Capn2,locus
R6247 T21169 T21161 auxpass was,detected
R6248 T21170 T21161 advmod initially,detected
R6249 T21171 T21161 agent by,detected
R6250 T21172 T21173 compound Southern,blotting
R6251 T21173 T21171 pobj blotting,by
R6252 T21174 T21161 punct .,detected
R6253 T21176 T21177 nsubjpass Membranes,blotted
R6254 T21178 T21177 auxpass were,blotted
R6255 T21179 T21177 prep with,blotted
R6256 T21180 T21181 npadvmod BamHI,digested
R6257 T21181 T21183 amod digested,DNA
R6258 T21182 T21181 punct -,digested
R6259 T21183 T21179 pobj DNA,with
R6260 T21184 T21183 amod genomic,DNA
R6261 T21185 T21183 acl extracted,DNA
R6262 T21186 T21185 prep from,extracted
R6263 T21187 T21188 compound ES,cells
R6264 T21188 T21186 pobj cells,from
R6265 T21189 T21177 cc and,blotted
R6266 T21190 T21177 conj hybridized,blotted
R6267 T21191 T21190 prep with,hybridized
R6268 T21192 T21193 det a,fragment
R6269 T21193 T21191 pobj fragment,with
R6270 T21194 T21195 npadvmod DIG,labeled
R6271 T21195 T21193 amod labeled,fragment
R6272 T21196 T21195 punct -,labeled
R6273 T21197 T21198 nummod 823,bp
R6274 T21198 T21193 compound bp,fragment
R6275 T21199 T21200 compound BamHI,HindIII
R6276 T21200 T21193 compound HindIII,fragment
R6277 T21201 T21200 punct /,HindIII
R6278 T21202 T21193 acl located,fragment
R6279 T21203 T21202 advmod immediately,located
R6280 T21204 T21203 advmod upstream,immediately
R6281 T21205 T21203 prep of,immediately
R6282 T21206 T21207 det the,arm
R6283 T21207 T21205 pobj arm,of
R6284 T21208 T21207 amod short,arm
R6285 T21209 T21207 prep of,arm
R6286 T21210 T21211 det the,vector
R6287 T21211 T21209 pobj vector,of
R6288 T21212 T21211 compound targeting,vector
R6289 T21213 T21214 punct (,Figure
R6290 T21214 T21190 parataxis Figure,hybridized
R6291 T21215 T21214 nummod 1,Figure
R6292 T21216 T21214 punct ),Figure
R6293 T21217 T21177 punct .,blotted
R6294 T21219 T21220 det A,fragment
R6295 T21220 T21225 nsubj fragment,was
R6296 T21221 T21222 nummod 3.5,kb
R6297 T21222 T21220 compound kb,fragment
R6298 T21223 T21222 punct -,kb
R6299 T21224 T21220 compound BamHI,fragment
R6300 T21226 T21220 acl corresponding,fragment
R6301 T21227 T21226 prep to,corresponding
R6302 T21228 T21229 det the,allele
R6303 T21229 T21227 pobj allele,to
R6304 T21230 T21231 amod wild,type
R6305 T21231 T21229 compound type,allele
R6306 T21232 T21231 punct -,type
R6307 T21233 T21225 acomp present,was
R6308 T21234 T21225 prep in,was
R6309 T21235 T21236 det all,cells
R6310 T21236 T21234 pobj cells,in
R6311 T21237 T21225 punct ", ",was
R6312 T21238 T21239 mark whereas,detected
R6313 T21239 T21225 advcl detected,was
R6314 T21240 T21241 det a,fragment
R6315 T21241 T21239 nsubjpass fragment,detected
R6316 T21242 T21243 nummod 5.3,kb
R6317 T21243 T21241 compound kb,fragment
R6318 T21244 T21243 punct -,kb
R6319 T21245 T21241 prep from,fragment
R6320 T21246 T21247 det the,allele
R6321 T21247 T21245 pobj allele,from
R6322 T21248 T21247 compound mutant,allele
R6323 T21249 T21239 auxpass was,detected
R6324 T21250 T21239 prep in,detected
R6325 T21251 T21252 nummod two,lines
R6326 T21252 T21250 pobj lines,in
R6327 T21253 T21252 amod targeted,lines
R6328 T21254 T21252 compound cell,lines
R6329 T21255 T21239 punct ", ",detected
R6330 T21256 T21239 dep designated,detected
R6331 T21257 T21256 oprd ES27,designated
R6332 T21258 T21257 cc and,ES27
R6333 T21259 T21257 conj ES36,ES27
R6334 T21260 T21225 punct .,was
R6335 T21262 T21263 punct (,B
R6336 T21263 T21264 meta B,carried
R6337 T21265 T21263 punct ),B
R6338 T21266 T21267 compound PCR,genotyping
R6339 T21267 T21264 nsubjpass genotyping,carried
R6340 T21268 T21264 auxpass was,carried
R6341 T21269 T21264 prt out,carried
R6342 T21270 T21264 prep with,carried
R6343 T21271 T21272 nummod two,reactions
R6344 T21272 T21270 pobj reactions,with
R6345 T21273 T21272 amod separate,reactions
R6346 T21274 T21272 acl designed,reactions
R6347 T21275 T21276 aux to,amplify
R6348 T21276 T21274 advcl amplify,designed
R6349 T21277 T21278 preconj either,segment
R6350 T21278 T21276 dobj segment,amplify
R6351 T21279 T21278 det a,segment
R6352 T21280 T21281 nummod "2,748",bp
R6353 T21281 T21278 compound bp,segment
R6354 T21282 T21278 prep from,segment
R6355 T21283 T21284 det the,allele
R6356 T21284 T21282 pobj allele,from
R6357 T21285 T21286 amod wild,type
R6358 T21286 T21284 compound type,allele
R6359 T21287 T21286 punct -,type
R6360 T21288 T21278 cc or,segment
R6361 T21289 T21290 det a,segment
R6362 T21290 T21278 conj segment,segment
R6363 T21291 T21292 nummod "2,711",bp
R6364 T21292 T21290 compound bp,segment
R6365 T21293 T21290 prep from,segment
R6366 T21294 T21295 det the,allele
R6367 T21295 T21293 pobj allele,from
R6368 T21296 T21295 compound mutant,allele
R6369 T21297 T21264 punct .,carried
R6370 T21299 T21300 det Both,reactions
R6371 T21300 T21301 nsubj reactions,used
R6372 T21302 T21303 det a,primer
R6373 T21303 T21301 dobj primer,used
R6374 T21304 T21305 amod common,sense
R6375 T21305 T21303 compound sense,primer
R6376 T21306 T21303 acl located,primer
R6377 T21307 T21306 prep in,located
R6378 T21308 T21307 pobj intron,in
R6379 T21309 T21308 nummod 4,intron
R6380 T21310 T21306 punct ", ",located
R6381 T21311 T21306 prep outside,located
R6382 T21312 T21313 det the,arm
R6383 T21313 T21311 pobj arm,outside
R6384 T21314 T21313 amod short,arm
R6385 T21315 T21313 prep of,arm
R6386 T21316 T21317 det the,vector
R6387 T21317 T21315 pobj vector,of
R6388 T21318 T21317 compound targeting,vector
R6389 T21319 T21313 punct ", ",arm
R6390 T21320 T21313 cc and,arm
R6391 T21321 T21322 amod distinct,primers
R6392 T21322 T21313 conj primers,arm
R6393 T21323 T21324 npadvmod allele,specific
R6394 T21324 T21322 amod specific,primers
R6395 T21325 T21324 punct -,specific
R6396 T21326 T21322 compound antisense,primers
R6397 T21327 T21301 punct .,used
R6398 T21329 T21330 det The,reaction
R6399 T21330 T21331 nsubj reaction,used
R6400 T21332 T21333 aux to,detect
R6401 T21333 T21330 advcl detect,reaction
R6402 T21334 T21335 det the,allele
R6403 T21335 T21333 dobj allele,detect
R6404 T21336 T21337 amod wild,type
R6405 T21337 T21335 compound type,allele
R6406 T21338 T21337 punct -,type
R6407 T21339 T21340 det an,primer
R6408 T21340 T21331 dobj primer,used
R6409 T21341 T21340 compound antisense,primer
R6410 T21342 T21340 acl located,primer
R6411 T21343 T21342 prep in,located
R6412 T21344 T21343 pobj exon,in
R6413 T21345 T21344 nummod 7,exon
R6414 T21346 T21347 mark while,done
R6415 T21347 T21331 advcl done,used
R6416 T21348 T21349 det the,amplification
R6417 T21349 T21347 nsubjpass amplification,done
R6418 T21350 T21349 prep of,amplification
R6419 T21351 T21352 det the,sequence
R6420 T21352 T21350 pobj sequence,of
R6421 T21353 T21352 compound mutant,sequence
R6422 T21354 T21347 auxpass was,done
R6423 T21355 T21347 prep with,done
R6424 T21356 T21357 det an,primer
R6425 T21357 T21355 pobj primer,with
R6426 T21358 T21357 compound antisense,primer
R6427 T21359 T21357 prep in,primer
R6428 T21360 T21361 det the,cassette
R6429 T21361 T21359 pobj cassette,in
R6430 T21362 T21363 compound PGK,Neo
R6431 T21363 T21361 compound Neo,cassette
R6432 T21364 T21363 punct -,Neo
R6433 T21365 T21331 punct .,used
R6434 T21367 T21368 det The,results
R6435 T21368 T21369 nsubj results,confirm
R6436 T21370 T21371 det the,presence
R6437 T21371 T21369 dobj presence,confirm
R6438 T21372 T21371 prep of,presence
R6439 T21373 T21374 det the,allele
R6440 T21374 T21372 pobj allele,of
R6441 T21375 T21376 amod wild,type
R6442 T21376 T21374 compound type,allele
R6443 T21377 T21376 punct -,type
R6444 T21378 T21371 prep in,presence
R6445 T21379 T21380 det all,cells
R6446 T21380 T21378 pobj cells,in
R6447 T21381 T21369 punct ", ",confirm
R6448 T21382 T21383 mark whereas,observed
R6449 T21383 T21369 advcl observed,confirm
R6450 T21384 T21385 det the,signal
R6451 T21385 T21383 nsubjpass signal,observed
R6452 T21386 T21385 compound mutant,signal
R6453 T21387 T21385 compound allele,signal
R6454 T21388 T21383 auxpass was,observed
R6455 T21389 T21390 advmod only,in
R6456 T21390 T21383 prep in,observed
R6457 T21391 T21392 det the,clones
R6458 T21392 T21390 pobj clones,in
R6459 T21393 T21392 nummod two,clones
R6460 T21394 T21392 amod targeted,clones
R6461 T21395 T21369 punct .,confirm
R6462 T21397 T21398 punct (,denotes
R6463 T21399 T21398 nsubj M,denotes
R6464 T21400 T21398 punct ),denotes
R6465 T21401 T21402 det the,marker
R6466 T21402 T21398 dobj marker,denotes
R6467 T21403 T21404 amod molecular,weight
R6468 T21404 T21402 compound weight,marker
R6469 T21405 T21398 punct .,denotes