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PMC:1382200 / 7143-8229 JSONTXT 4 Projects

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Id Subject Object Predicate Lexical cue
T4842 0-3 CD denotes Two
T4844 4-15 JJ denotes independent
T4845 16-21 NN denotes Capn2
T4846 21-22 SYM denotes +
T4847 22-23 HYPH denotes /
T4848 23-24 SYM denotes -
T4849 25-27 NN denotes ES
T4850 28-32 NN denotes cell
T4843 33-38 NNS denotes lines
T4852 38-40 , denotes ,
T4853 40-50 VBN denotes designated
T4854 51-55 NN denotes ES27
T4855 56-59 CC denotes and
T4856 60-64 NN denotes ES36
T4857 64-66 , denotes ,
T4858 66-70 VBD denotes were
T4851 71-79 VBN denotes isolated
T4859 80-84 IN denotes from
T4860 85-86 DT denotes a
T4861 87-93 NN denotes screen
T4862 94-96 IN denotes of
T4863 97-100 CD denotes 305
T4865 101-105 NN denotes drug
T4867 105-106 HYPH denotes -
T4866 106-115 JJ denotes resistant
T4864 116-122 NNS denotes clones
T4868 122-123 . denotes .
T4869 123-230 sentence denotes Correct targeting of the Capn2 locus was established both by Southern blot hybridization and PCR analysis.
T4870 124-131 JJ denotes Correct
T4871 132-141 NN denotes targeting
T4873 142-144 IN denotes of
T4874 145-148 DT denotes the
T4876 149-154 NN denotes Capn2
T4875 155-160 NN denotes locus
T4877 161-164 VBD denotes was
T4872 165-176 VBN denotes established
T4878 177-181 CC denotes both
T4879 182-184 IN denotes by
T4880 185-193 NNP denotes Southern
T4881 194-198 NN denotes blot
T4882 199-212 NN denotes hybridization
T4883 213-216 CC denotes and
T4884 217-220 NN denotes PCR
T4885 221-229 NN denotes analysis
T4886 229-230 . denotes .
T4887 230-444 sentence denotes A probe located outside the short (upstream) arm of homology hybridized to a 3.5-kb BamHI fragment of the wild-type allele and 5.3-kb BamHI fragment of the mutant allele as predicted from genomic maps (Figure 2A).
T4888 231-232 DT denotes A
T4889 233-238 NN denotes probe
T4891 239-246 VBN denotes located
T4892 247-254 RB denotes outside
T4893 255-258 DT denotes the
T4895 259-264 JJ denotes short
T4896 265-266 -LRB- denotes (
T4897 266-274 JJ denotes upstream
T4898 274-275 -RRB- denotes )
T4894 276-279 NN denotes arm
T4899 280-282 IN denotes of
T4900 283-291 NN denotes homology
T4890 292-302 VBN denotes hybridized
T4901 303-305 IN denotes to
T4902 306-307 DT denotes a
T4904 308-311 CD denotes 3.5
T4906 311-312 HYPH denotes -
T4905 312-314 NN denotes kb
T4907 315-320 NN denotes BamHI
T4903 321-329 NN denotes fragment
T4908 330-332 IN denotes of
T4909 333-336 DT denotes the
T4911 337-341 JJ denotes wild
T4913 341-342 HYPH denotes -
T4912 342-346 NN denotes type
T4910 347-353 NN denotes allele
T4914 354-357 CC denotes and
T4915 358-361 CD denotes 5.3
T4917 361-362 HYPH denotes -
T4916 362-364 NN denotes kb
T4919 365-370 NN denotes BamHI
T4918 371-379 NN denotes fragment
T4920 380-382 IN denotes of
T4921 383-386 DT denotes the
T4923 387-393 NN denotes mutant
T4922 394-400 NN denotes allele
T4924 401-403 IN denotes as
T4925 404-413 VBN denotes predicted
T4926 414-418 IN denotes from
T4927 419-426 JJ denotes genomic
T4928 427-431 NNS denotes maps
T4929 432-433 -LRB- denotes (
T4931 433-439 NN denotes Figure
T4930 440-442 NN denotes 2A
T4932 442-443 -RRB- denotes )
T4933 443-444 . denotes .
T4934 444-669 sentence denotes The same probe also detected the expected 7.2-kb wild-type and 6.4-kb mutant BglII fragments, 4.9-kb wild-type and 5.7-kb mutant NcoI fragments, as well as 7.2-kb wild-type and 4.9-kb mutant BglII/AgeI fragments (not shown).
T4935 445-448 DT denotes The
T4937 449-453 JJ denotes same
T4936 454-459 NN denotes probe
T4939 460-464 RB denotes also
T4938 465-473 VBD denotes detected
T4940 474-477 DT denotes the
T4942 478-486 VBN denotes expected
T4943 487-490 CD denotes 7.2
T4945 490-491 HYPH denotes -
T4944 491-493 NN denotes kb
T4946 494-498 JJ denotes wild
T4948 498-499 HYPH denotes -
T4947 499-503 NN denotes type
T4949 504-507 CC denotes and
T4950 508-511 CD denotes 6.4
T4952 511-512 HYPH denotes -
T4951 512-514 NN denotes kb
T4953 515-521 NN denotes mutant
T4954 522-527 NN denotes BglII
T4941 528-537 NNS denotes fragments
T4955 537-539 , denotes ,
T4956 539-542 CD denotes 4.9
T4958 542-543 HYPH denotes -
T4957 543-545 NN denotes kb
T4960 546-550 JJ denotes wild
T4962 550-551 HYPH denotes -
T4961 551-555 NN denotes type
T4963 556-559 CC denotes and
T4964 560-563 CD denotes 5.7
T4966 563-564 HYPH denotes -
T4965 564-566 NN denotes kb
T4967 567-573 NN denotes mutant
T4968 574-578 NN denotes NcoI
T4959 579-588 NNS denotes fragments
T4969 588-590 , denotes ,
T4970 590-592 RB denotes as
T4972 593-597 RB denotes well
T4971 598-600 IN denotes as
T4973 601-604 CD denotes 7.2
T4975 604-605 HYPH denotes -
T4974 605-607 NN denotes kb
T4977 608-612 JJ denotes wild
T4979 612-613 HYPH denotes -
T4978 613-617 NN denotes type
T4980 618-621 CC denotes and
T4981 622-625 CD denotes 4.9
T4983 625-626 HYPH denotes -
T4982 626-628 NN denotes kb
T4984 629-635 NN denotes mutant
T4985 636-641 NN denotes BglII
T4987 641-642 HYPH denotes /
T4986 642-646 NN denotes AgeI
T4976 647-656 NNS denotes fragments
T4988 657-658 -LRB- denotes (
T4990 658-661 RB denotes not
T4989 662-667 VBN denotes shown
T4991 667-668 -RRB- denotes )
T4992 668-669 . denotes .
T4993 669-866 sentence denotes A probe derived from the PGK-Neo cassette recognized only the 5.3-kb BamHI fragment in Capn2+/- ES cells, suggesting that the targeting vector had integrated solely at the Capn2 locus (not shown).
T4994 670-671 DT denotes A
T4995 672-677 NN denotes probe
T4997 678-685 VBN denotes derived
T4998 686-690 IN denotes from
T4999 691-694 DT denotes the
T5001 695-698 NN denotes PGK
T5003 698-699 HYPH denotes -
T5002 699-702 NN denotes Neo
T5000 703-711 NN denotes cassette
T4996 712-722 VBD denotes recognized
T5004 723-727 RB denotes only
T5006 728-731 DT denotes the
T5007 732-735 CD denotes 5.3
T5009 735-736 HYPH denotes -
T5008 736-738 NN denotes kb
T5010 739-744 NN denotes BamHI
T5005 745-753 NN denotes fragment
T5011 754-756 IN denotes in
T5012 757-762 NN denotes Capn2
T5014 762-763 SYM denotes +
T5015 763-764 HYPH denotes /
T5016 764-765 SYM denotes -
T5013 766-768 NN denotes ES
T5017 769-774 NNS denotes cells
T5018 774-776 , denotes ,
T5019 776-786 VBG denotes suggesting
T5020 787-791 IN denotes that
T5022 792-795 DT denotes the
T5024 796-805 NN denotes targeting
T5023 806-812 NN denotes vector
T5025 813-816 VBD denotes had
T5021 817-827 VBN denotes integrated
T5026 828-834 RB denotes solely
T5027 835-837 IN denotes at
T5028 838-841 DT denotes the
T5030 842-847 NN denotes Capn2
T5029 848-853 NN denotes locus
T5031 854-855 -LRB- denotes (
T5033 855-858 RB denotes not
T5032 859-864 VBN denotes shown
T5034 864-865 -RRB- denotes )
T5035 865-866 . denotes .
T5036 866-1004 sentence denotes A PCR screening method was also established that generated a wild-type product of 2,749 bp and a 2,711 bp product from the mutant allele.
T5037 867-868 DT denotes A
T5039 869-872 NN denotes PCR
T5040 873-882 NN denotes screening
T5038 883-889 NN denotes method
T5042 890-893 VBD denotes was
T5043 894-898 RB denotes also
T5041 899-910 VBN denotes established
T5044 911-915 WDT denotes that
T5045 916-925 VBD denotes generated
T5046 926-927 DT denotes a
T5048 928-932 JJ denotes wild
T5050 932-933 HYPH denotes -
T5049 933-937 NN denotes type
T5047 938-945 NN denotes product
T5051 946-948 IN denotes of
T5052 949-954 CD denotes 2,749
T5053 955-957 NN denotes bp
T5054 958-961 CC denotes and
T5055 962-963 DT denotes a
T5057 964-969 CD denotes 2,711
T5058 970-972 NN denotes bp
T5056 973-980 NN denotes product
T5059 981-985 IN denotes from
T5060 986-989 DT denotes the
T5062 990-996 NN denotes mutant
T5061 997-1003 NN denotes allele
T5063 1003-1004 . denotes .
T5064 1004-1086 sentence denotes The 2,711 bp product was only evident in the two targeted cell lines (Figure 2B).
T5065 1005-1008 DT denotes The
T5067 1009-1014 CD denotes 2,711
T5068 1015-1017 NN denotes bp
T5066 1018-1025 NN denotes product
T5069 1026-1029 VBD denotes was
T5070 1030-1034 RB denotes only
T5071 1035-1042 JJ denotes evident
T5072 1043-1045 IN denotes in
T5073 1046-1049 DT denotes the
T5075 1050-1053 CD denotes two
T5076 1054-1062 VBN denotes targeted
T5077 1063-1067 NN denotes cell
T5074 1068-1073 NNS denotes lines
T5078 1074-1075 -LRB- denotes (
T5080 1075-1081 NN denotes Figure
T5079 1082-1084 NN denotes 2B
T5081 1084-1085 -RRB- denotes )
T5082 1085-1086 . denotes .
R1315 T4842 T4843 nummod Two,lines
R1316 T4843 T4851 nsubjpass lines,isolated
R1317 T4844 T4843 amod independent,lines
R1318 T4845 T4843 nmod Capn2,lines
R1319 T4846 T4845 punct +,Capn2
R1320 T4847 T4845 punct /,Capn2
R1321 T4848 T4845 punct -,Capn2
R1322 T4849 T4843 compound ES,lines
R1323 T4850 T4843 compound cell,lines
R1324 T4852 T4843 punct ", ",lines
R1325 T4853 T4843 acl designated,lines
R1326 T4854 T4853 oprd ES27,designated
R1327 T4855 T4854 cc and,ES27
R1328 T4856 T4854 conj ES36,ES27
R1329 T4857 T4851 punct ", ",isolated
R1330 T4858 T4851 auxpass were,isolated
R1331 T4859 T4851 prep from,isolated
R1332 T4860 T4861 det a,screen
R1333 T4861 T4859 pobj screen,from
R1334 T4862 T4861 prep of,screen
R1335 T4863 T4864 nummod 305,clones
R1336 T4864 T4862 pobj clones,of
R1337 T4865 T4866 npadvmod drug,resistant
R1338 T4866 T4864 amod resistant,clones
R1339 T4867 T4866 punct -,resistant
R1340 T4868 T4851 punct .,isolated
R1341 T4870 T4871 amod Correct,targeting
R1342 T4871 T4872 nsubjpass targeting,established
R1343 T4873 T4871 prep of,targeting
R1344 T4874 T4875 det the,locus
R1345 T4875 T4873 pobj locus,of
R1346 T4876 T4875 compound Capn2,locus
R1347 T4877 T4872 auxpass was,established
R1348 T4878 T4879 preconj both,by
R1349 T4879 T4872 agent by,established
R1350 T4880 T4881 compound Southern,blot
R1351 T4881 T4882 compound blot,hybridization
R1352 T4882 T4879 pobj hybridization,by
R1353 T4883 T4882 cc and,hybridization
R1354 T4884 T4885 compound PCR,analysis
R1355 T4885 T4882 conj analysis,hybridization
R1356 T4886 T4872 punct .,established
R1357 T4888 T4889 det A,probe
R1358 T4889 T4890 nsubj probe,hybridized
R1359 T4891 T4889 acl located,probe
R1360 T4892 T4891 advmod outside,located
R1361 T4893 T4894 det the,arm
R1362 T4894 T4892 npadvmod arm,outside
R1363 T4895 T4894 amod short,arm
R1364 T4896 T4897 punct (,upstream
R1365 T4897 T4895 parataxis upstream,short
R1366 T4898 T4897 punct ),upstream
R1367 T4899 T4894 prep of,arm
R1368 T4900 T4899 pobj homology,of
R1369 T4901 T4890 prep to,hybridized
R1370 T4902 T4903 det a,fragment
R1371 T4903 T4901 pobj fragment,to
R1372 T4904 T4905 nummod 3.5,kb
R1373 T4905 T4903 compound kb,fragment
R1374 T4906 T4905 punct -,kb
R1375 T4907 T4903 compound BamHI,fragment
R1376 T4908 T4903 prep of,fragment
R1377 T4909 T4910 det the,allele
R1378 T4910 T4908 pobj allele,of
R1379 T4911 T4912 amod wild,type
R1380 T4912 T4910 compound type,allele
R1381 T4913 T4912 punct -,type
R1382 T4914 T4903 cc and,fragment
R1383 T4915 T4916 nummod 5.3,kb
R1384 T4916 T4918 compound kb,fragment
R1385 T4917 T4916 punct -,kb
R1386 T4918 T4903 conj fragment,fragment
R1387 T4919 T4918 compound BamHI,fragment
R1388 T4920 T4918 prep of,fragment
R1389 T4921 T4922 det the,allele
R1390 T4922 T4920 pobj allele,of
R1391 T4923 T4922 compound mutant,allele
R1392 T4924 T4925 mark as,predicted
R1393 T4925 T4890 advcl predicted,hybridized
R1394 T4926 T4925 prep from,predicted
R1395 T4927 T4928 amod genomic,maps
R1396 T4928 T4926 pobj maps,from
R1397 T4929 T4930 punct (,2A
R1398 T4930 T4925 parataxis 2A,predicted
R1399 T4931 T4930 compound Figure,2A
R1400 T4932 T4930 punct ),2A
R1401 T4933 T4890 punct .,hybridized
R1402 T4935 T4936 det The,probe
R1403 T4936 T4938 nsubj probe,detected
R1404 T4937 T4936 amod same,probe
R1405 T4939 T4938 advmod also,detected
R1406 T4940 T4941 det the,fragments
R1407 T4941 T4938 dobj fragments,detected
R1408 T4942 T4941 amod expected,fragments
R1409 T4943 T4944 nummod 7.2,kb
R1410 T4944 T4941 nmod kb,fragments
R1411 T4945 T4944 punct -,kb
R1412 T4946 T4947 amod wild,type
R1413 T4947 T4944 appos type,kb
R1414 T4948 T4947 punct -,type
R1415 T4949 T4944 cc and,kb
R1416 T4950 T4951 nummod 6.4,kb
R1417 T4951 T4953 compound kb,mutant
R1418 T4952 T4951 punct -,kb
R1419 T4953 T4944 conj mutant,kb
R1420 T4954 T4941 compound BglII,fragments
R1421 T4955 T4941 punct ", ",fragments
R1422 T4956 T4957 nummod 4.9,kb
R1423 T4957 T4959 nmod kb,fragments
R1424 T4958 T4957 punct -,kb
R1425 T4959 T4941 conj fragments,fragments
R1426 T4960 T4961 amod wild,type
R1427 T4961 T4957 appos type,kb
R1428 T4962 T4961 punct -,type
R1429 T4963 T4957 cc and,kb
R1430 T4964 T4965 nummod 5.7,kb
R1431 T4965 T4967 compound kb,mutant
R1432 T4966 T4965 punct -,kb
R1433 T4967 T4957 conj mutant,kb
R1434 T4968 T4959 compound NcoI,fragments
R1435 T4969 T4959 punct ", ",fragments
R1436 T4970 T4971 advmod as,as
R1437 T4971 T4959 cc as,fragments
R1438 T4972 T4971 advmod well,as
R1439 T4973 T4974 nummod 7.2,kb
R1440 T4974 T4976 nmod kb,fragments
R1441 T4975 T4974 punct -,kb
R1442 T4976 T4959 conj fragments,fragments
R1443 T4977 T4978 amod wild,type
R1444 T4978 T4974 appos type,kb
R1445 T4979 T4978 punct -,type
R1446 T4980 T4974 cc and,kb
R1447 T4981 T4982 nummod 4.9,kb
R1448 T4982 T4984 compound kb,mutant
R1449 T4983 T4982 punct -,kb
R1450 T4984 T4974 conj mutant,kb
R1451 T4985 T4986 compound BglII,AgeI
R1452 T4986 T4976 compound AgeI,fragments
R1453 T4987 T4986 punct /,AgeI
R1454 T4988 T4989 punct (,shown
R1455 T4989 T4938 parataxis shown,detected
R1456 T4990 T4989 neg not,shown
R1457 T4991 T4989 punct ),shown
R1458 T4992 T4938 punct .,detected
R1459 T4994 T4995 det A,probe
R1460 T4995 T4996 nsubj probe,recognized
R1461 T4997 T4995 acl derived,probe
R1462 T4998 T4997 prep from,derived
R1463 T4999 T5000 det the,cassette
R1464 T5000 T4998 pobj cassette,from
R1465 T5001 T5002 compound PGK,Neo
R1466 T5002 T5000 compound Neo,cassette
R1467 T5003 T5002 punct -,Neo
R1468 T5004 T5005 advmod only,fragment
R1469 T5005 T4996 dobj fragment,recognized
R1470 T5006 T5005 det the,fragment
R1471 T5007 T5008 nummod 5.3,kb
R1472 T5008 T5005 compound kb,fragment
R1473 T5009 T5008 punct -,kb
R1474 T5010 T5005 compound BamHI,fragment
R1475 T5011 T4996 prep in,recognized
R1476 T5012 T5013 nmod Capn2,ES
R1477 T5013 T5017 compound ES,cells
R1478 T5014 T5013 punct +,ES
R1479 T5015 T5013 punct /,ES
R1480 T5016 T5013 punct -,ES
R1481 T5017 T5011 pobj cells,in
R1482 T5018 T4996 punct ", ",recognized
R1483 T5019 T4996 advcl suggesting,recognized
R1484 T5020 T5021 mark that,integrated
R1485 T5021 T5019 ccomp integrated,suggesting
R1486 T5022 T5023 det the,vector
R1487 T5023 T5021 nsubj vector,integrated
R1488 T5024 T5023 compound targeting,vector
R1489 T5025 T5021 aux had,integrated
R1490 T5026 T5027 advmod solely,at
R1491 T5027 T5021 prep at,integrated
R1492 T5028 T5029 det the,locus
R1493 T5029 T5027 pobj locus,at
R1494 T5030 T5029 compound Capn2,locus
R1495 T5031 T5032 punct (,shown
R1496 T5032 T5019 parataxis shown,suggesting
R1497 T5033 T5032 neg not,shown
R1498 T5034 T5032 punct ),shown
R1499 T5035 T4996 punct .,recognized
R1500 T5037 T5038 det A,method
R1501 T5038 T5041 nsubjpass method,established
R1502 T5039 T5038 compound PCR,method
R1503 T5040 T5038 compound screening,method
R1504 T5042 T5041 auxpass was,established
R1505 T5043 T5041 advmod also,established
R1506 T5044 T5045 dep that,generated
R1507 T5045 T5041 ccomp generated,established
R1508 T5046 T5047 det a,product
R1509 T5047 T5045 dobj product,generated
R1510 T5048 T5049 amod wild,type
R1511 T5049 T5047 compound type,product
R1512 T5050 T5049 punct -,type
R1513 T5051 T5047 prep of,product
R1514 T5052 T5053 nummod "2,749",bp
R1515 T5053 T5051 pobj bp,of
R1516 T5054 T5047 cc and,product
R1517 T5055 T5056 det a,product
R1518 T5056 T5047 conj product,product
R1519 T5057 T5058 nummod "2,711",bp
R1520 T5058 T5056 compound bp,product
R1521 T5059 T5056 prep from,product
R1522 T5060 T5061 det the,allele
R1523 T5061 T5059 pobj allele,from
R1524 T5062 T5061 compound mutant,allele
R1525 T5063 T5041 punct .,established
R1526 T5065 T5066 det The,product
R1527 T5066 T5069 nsubj product,was
R1528 T5067 T5068 nummod "2,711",bp
R1529 T5068 T5066 compound bp,product
R1530 T5070 T5069 advmod only,was
R1531 T5071 T5069 acomp evident,was
R1532 T5072 T5069 prep in,was
R1533 T5073 T5074 det the,lines
R1534 T5074 T5072 pobj lines,in
R1535 T5075 T5074 nummod two,lines
R1536 T5076 T5074 amod targeted,lines
R1537 T5077 T5074 compound cell,lines
R1538 T5078 T5079 punct (,2B
R1539 T5079 T5069 parataxis 2B,was
R1540 T5080 T5079 compound Figure,2B
R1541 T5081 T5079 punct ),2B
R1542 T5082 T5069 punct .,was