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PMC:1382200 / 7079-9523 JSONTXT 4 Projects

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Id Subject Object Predicate Lexical cue
T4832 0-9 NN denotes Isolation
T4833 10-13 CC denotes and
T4834 14-30 NN denotes characterization
T4835 31-33 IN denotes of
T4836 34-39 NN denotes Capn2
T4837 40-48 VBN denotes targeted
T4839 49-51 NN denotes ES
T4840 52-56 NN denotes cell
T4838 57-63 NNS denotes clones
T4841 63-187 sentence denotes Two independent Capn2+/- ES cell lines, designated ES27 and ES36, were isolated from a screen of 305 drug-resistant clones.
T4842 64-67 CD denotes Two
T4844 68-79 JJ denotes independent
T4845 80-85 NN denotes Capn2
T4846 85-86 SYM denotes +
T4847 86-87 HYPH denotes /
T4848 87-88 SYM denotes -
T4849 89-91 NN denotes ES
T4850 92-96 NN denotes cell
T4843 97-102 NNS denotes lines
T4852 102-104 , denotes ,
T4853 104-114 VBN denotes designated
T4854 115-119 NN denotes ES27
T4855 120-123 CC denotes and
T4856 124-128 NN denotes ES36
T4857 128-130 , denotes ,
T4858 130-134 VBD denotes were
T4851 135-143 VBN denotes isolated
T4859 144-148 IN denotes from
T4860 149-150 DT denotes a
T4861 151-157 NN denotes screen
T4862 158-160 IN denotes of
T4863 161-164 CD denotes 305
T4865 165-169 NN denotes drug
T4867 169-170 HYPH denotes -
T4866 170-179 JJ denotes resistant
T4864 180-186 NNS denotes clones
T4868 186-187 . denotes .
T4869 187-294 sentence denotes Correct targeting of the Capn2 locus was established both by Southern blot hybridization and PCR analysis.
T4870 188-195 JJ denotes Correct
T4871 196-205 NN denotes targeting
T4873 206-208 IN denotes of
T4874 209-212 DT denotes the
T4876 213-218 NN denotes Capn2
T4875 219-224 NN denotes locus
T4877 225-228 VBD denotes was
T4872 229-240 VBN denotes established
T4878 241-245 CC denotes both
T4879 246-248 IN denotes by
T4880 249-257 NNP denotes Southern
T4881 258-262 NN denotes blot
T4882 263-276 NN denotes hybridization
T4883 277-280 CC denotes and
T4884 281-284 NN denotes PCR
T4885 285-293 NN denotes analysis
T4886 293-294 . denotes .
T4887 294-508 sentence denotes A probe located outside the short (upstream) arm of homology hybridized to a 3.5-kb BamHI fragment of the wild-type allele and 5.3-kb BamHI fragment of the mutant allele as predicted from genomic maps (Figure 2A).
T4888 295-296 DT denotes A
T4889 297-302 NN denotes probe
T4891 303-310 VBN denotes located
T4892 311-318 RB denotes outside
T4893 319-322 DT denotes the
T4895 323-328 JJ denotes short
T4896 329-330 -LRB- denotes (
T4897 330-338 JJ denotes upstream
T4898 338-339 -RRB- denotes )
T4894 340-343 NN denotes arm
T4899 344-346 IN denotes of
T4900 347-355 NN denotes homology
T4890 356-366 VBN denotes hybridized
T4901 367-369 IN denotes to
T4902 370-371 DT denotes a
T4904 372-375 CD denotes 3.5
T4906 375-376 HYPH denotes -
T4905 376-378 NN denotes kb
T4907 379-384 NN denotes BamHI
T4903 385-393 NN denotes fragment
T4908 394-396 IN denotes of
T4909 397-400 DT denotes the
T4911 401-405 JJ denotes wild
T4913 405-406 HYPH denotes -
T4912 406-410 NN denotes type
T4910 411-417 NN denotes allele
T4914 418-421 CC denotes and
T4915 422-425 CD denotes 5.3
T4917 425-426 HYPH denotes -
T4916 426-428 NN denotes kb
T4919 429-434 NN denotes BamHI
T4918 435-443 NN denotes fragment
T4920 444-446 IN denotes of
T4921 447-450 DT denotes the
T4923 451-457 NN denotes mutant
T4922 458-464 NN denotes allele
T4924 465-467 IN denotes as
T4925 468-477 VBN denotes predicted
T4926 478-482 IN denotes from
T4927 483-490 JJ denotes genomic
T4928 491-495 NNS denotes maps
T4929 496-497 -LRB- denotes (
T4931 497-503 NN denotes Figure
T4930 504-506 NN denotes 2A
T4932 506-507 -RRB- denotes )
T4933 507-508 . denotes .
T4934 508-733 sentence denotes The same probe also detected the expected 7.2-kb wild-type and 6.4-kb mutant BglII fragments, 4.9-kb wild-type and 5.7-kb mutant NcoI fragments, as well as 7.2-kb wild-type and 4.9-kb mutant BglII/AgeI fragments (not shown).
T4935 509-512 DT denotes The
T4937 513-517 JJ denotes same
T4936 518-523 NN denotes probe
T4939 524-528 RB denotes also
T4938 529-537 VBD denotes detected
T4940 538-541 DT denotes the
T4942 542-550 VBN denotes expected
T4943 551-554 CD denotes 7.2
T4945 554-555 HYPH denotes -
T4944 555-557 NN denotes kb
T4946 558-562 JJ denotes wild
T4948 562-563 HYPH denotes -
T4947 563-567 NN denotes type
T4949 568-571 CC denotes and
T4950 572-575 CD denotes 6.4
T4952 575-576 HYPH denotes -
T4951 576-578 NN denotes kb
T4953 579-585 NN denotes mutant
T4954 586-591 NN denotes BglII
T4941 592-601 NNS denotes fragments
T4955 601-603 , denotes ,
T4956 603-606 CD denotes 4.9
T4958 606-607 HYPH denotes -
T4957 607-609 NN denotes kb
T4960 610-614 JJ denotes wild
T4962 614-615 HYPH denotes -
T4961 615-619 NN denotes type
T4963 620-623 CC denotes and
T4964 624-627 CD denotes 5.7
T4966 627-628 HYPH denotes -
T4965 628-630 NN denotes kb
T4967 631-637 NN denotes mutant
T4968 638-642 NN denotes NcoI
T4959 643-652 NNS denotes fragments
T4969 652-654 , denotes ,
T4970 654-656 RB denotes as
T4972 657-661 RB denotes well
T4971 662-664 IN denotes as
T4973 665-668 CD denotes 7.2
T4975 668-669 HYPH denotes -
T4974 669-671 NN denotes kb
T4977 672-676 JJ denotes wild
T4979 676-677 HYPH denotes -
T4978 677-681 NN denotes type
T4980 682-685 CC denotes and
T4981 686-689 CD denotes 4.9
T4983 689-690 HYPH denotes -
T4982 690-692 NN denotes kb
T4984 693-699 NN denotes mutant
T4985 700-705 NN denotes BglII
T4987 705-706 HYPH denotes /
T4986 706-710 NN denotes AgeI
T4976 711-720 NNS denotes fragments
T4988 721-722 -LRB- denotes (
T4990 722-725 RB denotes not
T4989 726-731 VBN denotes shown
T4991 731-732 -RRB- denotes )
T4992 732-733 . denotes .
T4993 733-930 sentence denotes A probe derived from the PGK-Neo cassette recognized only the 5.3-kb BamHI fragment in Capn2+/- ES cells, suggesting that the targeting vector had integrated solely at the Capn2 locus (not shown).
T4994 734-735 DT denotes A
T4995 736-741 NN denotes probe
T4997 742-749 VBN denotes derived
T4998 750-754 IN denotes from
T4999 755-758 DT denotes the
T5001 759-762 NN denotes PGK
T5003 762-763 HYPH denotes -
T5002 763-766 NN denotes Neo
T5000 767-775 NN denotes cassette
T4996 776-786 VBD denotes recognized
T5004 787-791 RB denotes only
T5006 792-795 DT denotes the
T5007 796-799 CD denotes 5.3
T5009 799-800 HYPH denotes -
T5008 800-802 NN denotes kb
T5010 803-808 NN denotes BamHI
T5005 809-817 NN denotes fragment
T5011 818-820 IN denotes in
T5012 821-826 NN denotes Capn2
T5014 826-827 SYM denotes +
T5015 827-828 HYPH denotes /
T5016 828-829 SYM denotes -
T5013 830-832 NN denotes ES
T5017 833-838 NNS denotes cells
T5018 838-840 , denotes ,
T5019 840-850 VBG denotes suggesting
T5020 851-855 IN denotes that
T5022 856-859 DT denotes the
T5024 860-869 NN denotes targeting
T5023 870-876 NN denotes vector
T5025 877-880 VBD denotes had
T5021 881-891 VBN denotes integrated
T5026 892-898 RB denotes solely
T5027 899-901 IN denotes at
T5028 902-905 DT denotes the
T5030 906-911 NN denotes Capn2
T5029 912-917 NN denotes locus
T5031 918-919 -LRB- denotes (
T5033 919-922 RB denotes not
T5032 923-928 VBN denotes shown
T5034 928-929 -RRB- denotes )
T5035 929-930 . denotes .
T5036 930-1068 sentence denotes A PCR screening method was also established that generated a wild-type product of 2,749 bp and a 2,711 bp product from the mutant allele.
T5037 931-932 DT denotes A
T5039 933-936 NN denotes PCR
T5040 937-946 NN denotes screening
T5038 947-953 NN denotes method
T5042 954-957 VBD denotes was
T5043 958-962 RB denotes also
T5041 963-974 VBN denotes established
T5044 975-979 WDT denotes that
T5045 980-989 VBD denotes generated
T5046 990-991 DT denotes a
T5048 992-996 JJ denotes wild
T5050 996-997 HYPH denotes -
T5049 997-1001 NN denotes type
T5047 1002-1009 NN denotes product
T5051 1010-1012 IN denotes of
T5052 1013-1018 CD denotes 2,749
T5053 1019-1021 NN denotes bp
T5054 1022-1025 CC denotes and
T5055 1026-1027 DT denotes a
T5057 1028-1033 CD denotes 2,711
T5058 1034-1036 NN denotes bp
T5056 1037-1044 NN denotes product
T5059 1045-1049 IN denotes from
T5060 1050-1053 DT denotes the
T5062 1054-1060 NN denotes mutant
T5061 1061-1067 NN denotes allele
T5063 1067-1068 . denotes .
T5064 1068-1150 sentence denotes The 2,711 bp product was only evident in the two targeted cell lines (Figure 2B).
T5065 1069-1072 DT denotes The
T5067 1073-1078 CD denotes 2,711
T5068 1079-1081 NN denotes bp
T5066 1082-1089 NN denotes product
T5069 1090-1093 VBD denotes was
T5070 1094-1098 RB denotes only
T5071 1099-1106 JJ denotes evident
T5072 1107-1109 IN denotes in
T5073 1110-1113 DT denotes the
T5075 1114-1117 CD denotes two
T5076 1118-1126 VBN denotes targeted
T5077 1127-1131 NN denotes cell
T5074 1132-1137 NNS denotes lines
T5078 1138-1139 -LRB- denotes (
T5080 1139-1145 NN denotes Figure
T5079 1146-1148 NN denotes 2B
T5081 1148-1149 -RRB- denotes )
T5082 1149-1150 . denotes .
T21151 1161-1177 NN denotes Characterization
T21152 1178-1180 IN denotes of
T21153 1181-1189 VBN denotes targeted
T21155 1190-1192 NN denotes ES
T21156 1193-1197 NN denotes cell
T21154 1198-1203 NNS denotes lines
T21157 1203-1204 . denotes .
T21158 1204-1292 sentence denotes (A) Targeted disruption of the Capn2 locus was detected initially by Southern blotting.
T21159 1205-1206 -LRB- denotes (
T21160 1206-1207 LS denotes A
T21162 1207-1208 -RRB- denotes )
T21163 1209-1217 VBN denotes Targeted
T21164 1218-1228 NN denotes disruption
T21165 1229-1231 IN denotes of
T21166 1232-1235 DT denotes the
T21168 1236-1241 NN denotes Capn2
T21167 1242-1247 NN denotes locus
T21169 1248-1251 VBD denotes was
T21161 1252-1260 VBN denotes detected
T21170 1261-1270 RB denotes initially
T21171 1271-1273 IN denotes by
T21172 1274-1282 NNP denotes Southern
T21173 1283-1291 NN denotes blotting
T21174 1291-1292 . denotes .
T21175 1292-1517 sentence denotes Membranes were blotted with BamHI-digested genomic DNA extracted from ES cells and hybridized with a DIG-labeled 823 bp BamHI/HindIII fragment located immediately upstream of the short arm of the targeting vector (Figure 1).
T21176 1293-1302 NNS denotes Membranes
T21178 1303-1307 VBD denotes were
T21177 1308-1315 VBN denotes blotted
T21179 1316-1320 IN denotes with
T21180 1321-1326 NN denotes BamHI
T21182 1326-1327 HYPH denotes -
T21181 1327-1335 VBN denotes digested
T21184 1336-1343 JJ denotes genomic
T21183 1344-1347 NN denotes DNA
T21185 1348-1357 VBN denotes extracted
T21186 1358-1362 IN denotes from
T21187 1363-1365 NN denotes ES
T21188 1366-1371 NNS denotes cells
T21189 1372-1375 CC denotes and
T21190 1376-1386 VBD denotes hybridized
T21191 1387-1391 IN denotes with
T21192 1392-1393 DT denotes a
T21194 1394-1397 NN denotes DIG
T21196 1397-1398 HYPH denotes -
T21195 1398-1405 VBN denotes labeled
T21197 1406-1409 CD denotes 823
T21198 1410-1412 NN denotes bp
T21199 1413-1418 NN denotes BamHI
T21201 1418-1419 HYPH denotes /
T21200 1419-1426 NN denotes HindIII
T21193 1427-1435 NN denotes fragment
T21202 1436-1443 VBN denotes located
T21203 1444-1455 RB denotes immediately
T21204 1456-1464 IN denotes upstream
T21205 1465-1467 IN denotes of
T21206 1468-1471 DT denotes the
T21208 1472-1477 JJ denotes short
T21207 1478-1481 NN denotes arm
T21209 1482-1484 IN denotes of
T21210 1485-1488 DT denotes the
T21212 1489-1498 NN denotes targeting
T21211 1499-1505 NN denotes vector
T21213 1506-1507 -LRB- denotes (
T21214 1507-1513 NN denotes Figure
T21215 1514-1515 CD denotes 1
T21216 1515-1516 -RRB- denotes )
T21217 1516-1517 . denotes .
T21218 1517-1721 sentence denotes A 3.5-kb BamHI fragment corresponding to the wild-type allele was present in all cells, whereas a 5.3-kb fragment from the mutant allele was detected in two targeted cell lines, designated ES27 and ES36.
T21219 1518-1519 DT denotes A
T21221 1520-1523 CD denotes 3.5
T21223 1523-1524 HYPH denotes -
T21222 1524-1526 NN denotes kb
T21224 1527-1532 NN denotes BamHI
T21220 1533-1541 NN denotes fragment
T21226 1542-1555 VBG denotes corresponding
T21227 1556-1558 IN denotes to
T21228 1559-1562 DT denotes the
T21230 1563-1567 JJ denotes wild
T21232 1567-1568 HYPH denotes -
T21231 1568-1572 NN denotes type
T21229 1573-1579 NN denotes allele
T21225 1580-1583 VBD denotes was
T21233 1584-1591 JJ denotes present
T21234 1592-1594 IN denotes in
T21235 1595-1598 DT denotes all
T21236 1599-1604 NNS denotes cells
T21237 1604-1606 , denotes ,
T21238 1606-1613 IN denotes whereas
T21240 1614-1615 DT denotes a
T21242 1616-1619 CD denotes 5.3
T21244 1619-1620 HYPH denotes -
T21243 1620-1622 NN denotes kb
T21241 1623-1631 NN denotes fragment
T21245 1632-1636 IN denotes from
T21246 1637-1640 DT denotes the
T21248 1641-1647 NN denotes mutant
T21247 1648-1654 NN denotes allele
T21249 1655-1658 VBD denotes was
T21239 1659-1667 VBN denotes detected
T21250 1668-1670 IN denotes in
T21251 1671-1674 CD denotes two
T21253 1675-1683 VBN denotes targeted
T21254 1684-1688 NN denotes cell
T21252 1689-1694 NNS denotes lines
T21255 1694-1696 , denotes ,
T21256 1696-1706 VBN denotes designated
T21257 1707-1711 NN denotes ES27
T21258 1712-1715 CC denotes and
T21259 1716-1720 NN denotes ES36
T21260 1720-1721 . denotes .
T21261 1721-1902 sentence denotes (B) PCR genotyping was carried out with two separate reactions designed to amplify either a 2,748 bp segment from the wild-type allele or a 2,711 bp segment from the mutant allele.
T21262 1722-1723 -LRB- denotes (
T21263 1723-1724 LS denotes B
T21265 1724-1725 -RRB- denotes )
T21266 1726-1729 NN denotes PCR
T21267 1730-1740 NN denotes genotyping
T21268 1741-1744 VBD denotes was
T21264 1745-1752 VBN denotes carried
T21269 1753-1756 RP denotes out
T21270 1757-1761 IN denotes with
T21271 1762-1765 CD denotes two
T21273 1766-1774 JJ denotes separate
T21272 1775-1784 NNS denotes reactions
T21274 1785-1793 VBN denotes designed
T21275 1794-1796 TO denotes to
T21276 1797-1804 VB denotes amplify
T21277 1805-1811 CC denotes either
T21279 1812-1813 DT denotes a
T21280 1814-1819 CD denotes 2,748
T21281 1820-1822 NN denotes bp
T21278 1823-1830 NN denotes segment
T21282 1831-1835 IN denotes from
T21283 1836-1839 DT denotes the
T21285 1840-1844 JJ denotes wild
T21287 1844-1845 HYPH denotes -
T21286 1845-1849 NN denotes type
T21284 1850-1856 NN denotes allele
T21288 1857-1859 CC denotes or
T21289 1860-1861 DT denotes a
T21291 1862-1867 CD denotes 2,711
T21292 1868-1870 NN denotes bp
T21290 1871-1878 NN denotes segment
T21293 1879-1883 IN denotes from
T21294 1884-1887 DT denotes the
T21296 1888-1894 NN denotes mutant
T21295 1895-1901 NN denotes allele
T21297 1901-1902 . denotes .
T21298 1902-2060 sentence denotes Both reactions used a common sense primer located in intron 4, outside the short arm of the targeting vector, and distinct allele-specific antisense primers.
T21299 1903-1907 DT denotes Both
T21300 1908-1917 NNS denotes reactions
T21301 1918-1922 VBD denotes used
T21302 1923-1924 DT denotes a
T21304 1925-1931 JJ denotes common
T21305 1932-1937 NN denotes sense
T21303 1938-1944 NN denotes primer
T21306 1945-1952 VBN denotes located
T21307 1953-1955 IN denotes in
T21308 1956-1962 NN denotes intron
T21309 1963-1964 CD denotes 4
T21310 1964-1966 , denotes ,
T21311 1966-1973 IN denotes outside
T21312 1974-1977 DT denotes the
T21314 1978-1983 JJ denotes short
T21313 1984-1987 NN denotes arm
T21315 1988-1990 IN denotes of
T21316 1991-1994 DT denotes the
T21318 1995-2004 NN denotes targeting
T21317 2005-2011 NN denotes vector
T21319 2011-2013 , denotes ,
T21320 2013-2016 CC denotes and
T21321 2017-2025 JJ denotes distinct
T21323 2026-2032 NN denotes allele
T21325 2032-2033 HYPH denotes -
T21324 2033-2041 JJ denotes specific
T21326 2042-2051 NN denotes antisense
T21322 2052-2059 NNS denotes primers
T21327 2059-2060 . denotes .
T21328 2060-2253 sentence denotes The reaction to detect the wild-type allele used an antisense primer located in exon 7 while the amplification of the mutant sequence was done with an antisense primer in the PGK-Neo cassette.
T21329 2061-2064 DT denotes The
T21330 2065-2073 NN denotes reaction
T21332 2074-2076 TO denotes to
T21333 2077-2083 VB denotes detect
T21334 2084-2087 DT denotes the
T21336 2088-2092 JJ denotes wild
T21338 2092-2093 HYPH denotes -
T21337 2093-2097 NN denotes type
T21335 2098-2104 NN denotes allele
T21331 2105-2109 VBD denotes used
T21339 2110-2112 DT denotes an
T21341 2113-2122 NN denotes antisense
T21340 2123-2129 NN denotes primer
T21342 2130-2137 VBN denotes located
T21343 2138-2140 IN denotes in
T21344 2141-2145 NN denotes exon
T21345 2146-2147 CD denotes 7
T21346 2148-2153 IN denotes while
T21348 2154-2157 DT denotes the
T21349 2158-2171 NN denotes amplification
T21350 2172-2174 IN denotes of
T21351 2175-2178 DT denotes the
T21353 2179-2185 NN denotes mutant
T21352 2186-2194 NN denotes sequence
T21354 2195-2198 VBD denotes was
T21347 2199-2203 VBN denotes done
T21355 2204-2208 IN denotes with
T21356 2209-2211 DT denotes an
T21358 2212-2221 NN denotes antisense
T21357 2222-2228 NN denotes primer
T21359 2229-2231 IN denotes in
T21360 2232-2235 DT denotes the
T21362 2236-2239 NN denotes PGK
T21364 2239-2240 HYPH denotes -
T21363 2240-2243 NN denotes Neo
T21361 2244-2252 NN denotes cassette
T21365 2252-2253 . denotes .
T21366 2253-2403 sentence denotes The results confirm the presence of the wild-type allele in all cells, whereas the mutant allele signal was observed only in the two targeted clones.
T21367 2254-2257 DT denotes The
T21368 2258-2265 NNS denotes results
T21369 2266-2273 VBP denotes confirm
T21370 2274-2277 DT denotes the
T21371 2278-2286 NN denotes presence
T21372 2287-2289 IN denotes of
T21373 2290-2293 DT denotes the
T21375 2294-2298 JJ denotes wild
T21377 2298-2299 HYPH denotes -
T21376 2299-2303 NN denotes type
T21374 2304-2310 NN denotes allele
T21378 2311-2313 IN denotes in
T21379 2314-2317 DT denotes all
T21380 2318-2323 NNS denotes cells
T21381 2323-2325 , denotes ,
T21382 2325-2332 IN denotes whereas
T21384 2333-2336 DT denotes the
T21386 2337-2343 NN denotes mutant
T21387 2344-2350 NN denotes allele
T21385 2351-2357 NN denotes signal
T21388 2358-2361 VBD denotes was
T21383 2362-2370 VBN denotes observed
T21389 2371-2375 RB denotes only
T21390 2376-2378 IN denotes in
T21391 2379-2382 DT denotes the
T21393 2383-2386 CD denotes two
T21394 2387-2395 VBN denotes targeted
T21392 2396-2402 NNS denotes clones
T21395 2402-2403 . denotes .
T21396 2403-2444 sentence denotes (M) denotes the molecular weight marker.
T21397 2404-2405 -LRB- denotes (
T21399 2405-2406 NN denotes M
T21400 2406-2407 -RRB- denotes )
T21398 2408-2415 VBZ denotes denotes
T21401 2416-2419 DT denotes the
T21403 2420-2429 JJ denotes molecular
T21404 2430-2436 NN denotes weight
T21402 2437-2443 NN denotes marker
T21405 2443-2444 . denotes .
R1307 T4833 T4832 cc and,Isolation
R1308 T4834 T4832 conj characterization,Isolation
R1309 T4835 T4832 prep of,Isolation
R1310 T4836 T4837 npadvmod Capn2,targeted
R1311 T4837 T4838 amod targeted,clones
R1312 T4838 T4835 pobj clones,of
R1313 T4839 T4840 compound ES,cell
R1314 T4840 T4838 compound cell,clones
R1315 T4842 T4843 nummod Two,lines
R1316 T4843 T4851 nsubjpass lines,isolated
R1317 T4844 T4843 amod independent,lines
R1318 T4845 T4843 nmod Capn2,lines
R1319 T4846 T4845 punct +,Capn2
R1320 T4847 T4845 punct /,Capn2
R1321 T4848 T4845 punct -,Capn2
R1322 T4849 T4843 compound ES,lines
R1323 T4850 T4843 compound cell,lines
R1324 T4852 T4843 punct ", ",lines
R1325 T4853 T4843 acl designated,lines
R1326 T4854 T4853 oprd ES27,designated
R1327 T4855 T4854 cc and,ES27
R1328 T4856 T4854 conj ES36,ES27
R1329 T4857 T4851 punct ", ",isolated
R1330 T4858 T4851 auxpass were,isolated
R1331 T4859 T4851 prep from,isolated
R1332 T4860 T4861 det a,screen
R1333 T4861 T4859 pobj screen,from
R1334 T4862 T4861 prep of,screen
R1335 T4863 T4864 nummod 305,clones
R1336 T4864 T4862 pobj clones,of
R1337 T4865 T4866 npadvmod drug,resistant
R1338 T4866 T4864 amod resistant,clones
R1339 T4867 T4866 punct -,resistant
R1340 T4868 T4851 punct .,isolated
R1341 T4870 T4871 amod Correct,targeting
R1342 T4871 T4872 nsubjpass targeting,established
R1343 T4873 T4871 prep of,targeting
R1344 T4874 T4875 det the,locus
R1345 T4875 T4873 pobj locus,of
R1346 T4876 T4875 compound Capn2,locus
R1347 T4877 T4872 auxpass was,established
R1348 T4878 T4879 preconj both,by
R1349 T4879 T4872 agent by,established
R1350 T4880 T4881 compound Southern,blot
R1351 T4881 T4882 compound blot,hybridization
R1352 T4882 T4879 pobj hybridization,by
R1353 T4883 T4882 cc and,hybridization
R1354 T4884 T4885 compound PCR,analysis
R1355 T4885 T4882 conj analysis,hybridization
R1356 T4886 T4872 punct .,established
R1357 T4888 T4889 det A,probe
R1358 T4889 T4890 nsubj probe,hybridized
R1359 T4891 T4889 acl located,probe
R1360 T4892 T4891 advmod outside,located
R1361 T4893 T4894 det the,arm
R1362 T4894 T4892 npadvmod arm,outside
R1363 T4895 T4894 amod short,arm
R1364 T4896 T4897 punct (,upstream
R1365 T4897 T4895 parataxis upstream,short
R1366 T4898 T4897 punct ),upstream
R1367 T4899 T4894 prep of,arm
R1368 T4900 T4899 pobj homology,of
R1369 T4901 T4890 prep to,hybridized
R1370 T4902 T4903 det a,fragment
R1371 T4903 T4901 pobj fragment,to
R1372 T4904 T4905 nummod 3.5,kb
R1373 T4905 T4903 compound kb,fragment
R1374 T4906 T4905 punct -,kb
R1375 T4907 T4903 compound BamHI,fragment
R1376 T4908 T4903 prep of,fragment
R1377 T4909 T4910 det the,allele
R1378 T4910 T4908 pobj allele,of
R1379 T4911 T4912 amod wild,type
R1380 T4912 T4910 compound type,allele
R1381 T4913 T4912 punct -,type
R1382 T4914 T4903 cc and,fragment
R1383 T4915 T4916 nummod 5.3,kb
R1384 T4916 T4918 compound kb,fragment
R1385 T4917 T4916 punct -,kb
R1386 T4918 T4903 conj fragment,fragment
R1387 T4919 T4918 compound BamHI,fragment
R1388 T4920 T4918 prep of,fragment
R1389 T4921 T4922 det the,allele
R1390 T4922 T4920 pobj allele,of
R1391 T4923 T4922 compound mutant,allele
R1392 T4924 T4925 mark as,predicted
R1393 T4925 T4890 advcl predicted,hybridized
R1394 T4926 T4925 prep from,predicted
R1395 T4927 T4928 amod genomic,maps
R1396 T4928 T4926 pobj maps,from
R1397 T4929 T4930 punct (,2A
R1398 T4930 T4925 parataxis 2A,predicted
R1399 T4931 T4930 compound Figure,2A
R1400 T4932 T4930 punct ),2A
R1401 T4933 T4890 punct .,hybridized
R1402 T4935 T4936 det The,probe
R1403 T4936 T4938 nsubj probe,detected
R1404 T4937 T4936 amod same,probe
R1405 T4939 T4938 advmod also,detected
R1406 T4940 T4941 det the,fragments
R1407 T4941 T4938 dobj fragments,detected
R1408 T4942 T4941 amod expected,fragments
R1409 T4943 T4944 nummod 7.2,kb
R1410 T4944 T4941 nmod kb,fragments
R1411 T4945 T4944 punct -,kb
R1412 T4946 T4947 amod wild,type
R1413 T4947 T4944 appos type,kb
R1414 T4948 T4947 punct -,type
R1415 T4949 T4944 cc and,kb
R1416 T4950 T4951 nummod 6.4,kb
R1417 T4951 T4953 compound kb,mutant
R1418 T4952 T4951 punct -,kb
R1419 T4953 T4944 conj mutant,kb
R1420 T4954 T4941 compound BglII,fragments
R1421 T4955 T4941 punct ", ",fragments
R1422 T4956 T4957 nummod 4.9,kb
R1423 T4957 T4959 nmod kb,fragments
R1424 T4958 T4957 punct -,kb
R1425 T4959 T4941 conj fragments,fragments
R1426 T4960 T4961 amod wild,type
R1427 T4961 T4957 appos type,kb
R1428 T4962 T4961 punct -,type
R1429 T4963 T4957 cc and,kb
R1430 T4964 T4965 nummod 5.7,kb
R1431 T4965 T4967 compound kb,mutant
R1432 T4966 T4965 punct -,kb
R1433 T4967 T4957 conj mutant,kb
R1434 T4968 T4959 compound NcoI,fragments
R1435 T4969 T4959 punct ", ",fragments
R1436 T4970 T4971 advmod as,as
R1437 T4971 T4959 cc as,fragments
R1438 T4972 T4971 advmod well,as
R1439 T4973 T4974 nummod 7.2,kb
R1440 T4974 T4976 nmod kb,fragments
R1441 T4975 T4974 punct -,kb
R1442 T4976 T4959 conj fragments,fragments
R1443 T4977 T4978 amod wild,type
R1444 T4978 T4974 appos type,kb
R1445 T4979 T4978 punct -,type
R1446 T4980 T4974 cc and,kb
R1447 T4981 T4982 nummod 4.9,kb
R1448 T4982 T4984 compound kb,mutant
R1449 T4983 T4982 punct -,kb
R1450 T4984 T4974 conj mutant,kb
R1451 T4985 T4986 compound BglII,AgeI
R1452 T4986 T4976 compound AgeI,fragments
R1453 T4987 T4986 punct /,AgeI
R1454 T4988 T4989 punct (,shown
R1455 T4989 T4938 parataxis shown,detected
R1456 T4990 T4989 neg not,shown
R1457 T4991 T4989 punct ),shown
R1458 T4992 T4938 punct .,detected
R1459 T4994 T4995 det A,probe
R1460 T4995 T4996 nsubj probe,recognized
R1461 T4997 T4995 acl derived,probe
R1462 T4998 T4997 prep from,derived
R1463 T4999 T5000 det the,cassette
R1464 T5000 T4998 pobj cassette,from
R1465 T5001 T5002 compound PGK,Neo
R1466 T5002 T5000 compound Neo,cassette
R1467 T5003 T5002 punct -,Neo
R1468 T5004 T5005 advmod only,fragment
R1469 T5005 T4996 dobj fragment,recognized
R1470 T5006 T5005 det the,fragment
R1471 T5007 T5008 nummod 5.3,kb
R1472 T5008 T5005 compound kb,fragment
R1473 T5009 T5008 punct -,kb
R1474 T5010 T5005 compound BamHI,fragment
R1475 T5011 T4996 prep in,recognized
R1476 T5012 T5013 nmod Capn2,ES
R1477 T5013 T5017 compound ES,cells
R1478 T5014 T5013 punct +,ES
R1479 T5015 T5013 punct /,ES
R1480 T5016 T5013 punct -,ES
R1481 T5017 T5011 pobj cells,in
R1482 T5018 T4996 punct ", ",recognized
R1483 T5019 T4996 advcl suggesting,recognized
R1484 T5020 T5021 mark that,integrated
R1485 T5021 T5019 ccomp integrated,suggesting
R1486 T5022 T5023 det the,vector
R1487 T5023 T5021 nsubj vector,integrated
R1488 T5024 T5023 compound targeting,vector
R1489 T5025 T5021 aux had,integrated
R1490 T5026 T5027 advmod solely,at
R1491 T5027 T5021 prep at,integrated
R1492 T5028 T5029 det the,locus
R1493 T5029 T5027 pobj locus,at
R1494 T5030 T5029 compound Capn2,locus
R1495 T5031 T5032 punct (,shown
R1496 T5032 T5019 parataxis shown,suggesting
R1497 T5033 T5032 neg not,shown
R1498 T5034 T5032 punct ),shown
R1499 T5035 T4996 punct .,recognized
R1500 T5037 T5038 det A,method
R1501 T5038 T5041 nsubjpass method,established
R1502 T5039 T5038 compound PCR,method
R1503 T5040 T5038 compound screening,method
R1504 T5042 T5041 auxpass was,established
R1505 T5043 T5041 advmod also,established
R1506 T5044 T5045 dep that,generated
R1507 T5045 T5041 ccomp generated,established
R1508 T5046 T5047 det a,product
R1509 T5047 T5045 dobj product,generated
R1510 T5048 T5049 amod wild,type
R1511 T5049 T5047 compound type,product
R1512 T5050 T5049 punct -,type
R1513 T5051 T5047 prep of,product
R1514 T5052 T5053 nummod "2,749",bp
R1515 T5053 T5051 pobj bp,of
R1516 T5054 T5047 cc and,product
R1517 T5055 T5056 det a,product
R1518 T5056 T5047 conj product,product
R1519 T5057 T5058 nummod "2,711",bp
R1520 T5058 T5056 compound bp,product
R1521 T5059 T5056 prep from,product
R1522 T5060 T5061 det the,allele
R1523 T5061 T5059 pobj allele,from
R1524 T5062 T5061 compound mutant,allele
R1525 T5063 T5041 punct .,established
R1526 T5065 T5066 det The,product
R1527 T5066 T5069 nsubj product,was
R1528 T5067 T5068 nummod "2,711",bp
R1529 T5068 T5066 compound bp,product
R1530 T5070 T5069 advmod only,was
R1531 T5071 T5069 acomp evident,was
R1532 T5072 T5069 prep in,was
R1533 T5073 T5074 det the,lines
R1534 T5074 T5072 pobj lines,in
R1535 T5075 T5074 nummod two,lines
R1536 T5076 T5074 amod targeted,lines
R1537 T5077 T5074 compound cell,lines
R1538 T5078 T5079 punct (,2B
R1539 T5079 T5069 parataxis 2B,was
R1540 T5080 T5079 compound Figure,2B
R1541 T5081 T5079 punct ),2B
R1542 T5082 T5069 punct .,was
R6232 T21152 T21151 prep of,Characterization
R6233 T21153 T21154 amod targeted,lines
R6234 T21154 T21152 pobj lines,of
R6235 T21155 T21156 compound ES,cell
R6236 T21156 T21154 compound cell,lines
R6237 T21157 T21151 punct .,Characterization
R6238 T21159 T21160 punct (,A
R6239 T21160 T21161 meta A,detected
R6240 T21162 T21160 punct ),A
R6241 T21163 T21164 amod Targeted,disruption
R6242 T21164 T21161 nsubjpass disruption,detected
R6243 T21165 T21164 prep of,disruption
R6244 T21166 T21167 det the,locus
R6245 T21167 T21165 pobj locus,of
R6246 T21168 T21167 compound Capn2,locus
R6247 T21169 T21161 auxpass was,detected
R6248 T21170 T21161 advmod initially,detected
R6249 T21171 T21161 agent by,detected
R6250 T21172 T21173 compound Southern,blotting
R6251 T21173 T21171 pobj blotting,by
R6252 T21174 T21161 punct .,detected
R6253 T21176 T21177 nsubjpass Membranes,blotted
R6254 T21178 T21177 auxpass were,blotted
R6255 T21179 T21177 prep with,blotted
R6256 T21180 T21181 npadvmod BamHI,digested
R6257 T21181 T21183 amod digested,DNA
R6258 T21182 T21181 punct -,digested
R6259 T21183 T21179 pobj DNA,with
R6260 T21184 T21183 amod genomic,DNA
R6261 T21185 T21183 acl extracted,DNA
R6262 T21186 T21185 prep from,extracted
R6263 T21187 T21188 compound ES,cells
R6264 T21188 T21186 pobj cells,from
R6265 T21189 T21177 cc and,blotted
R6266 T21190 T21177 conj hybridized,blotted
R6267 T21191 T21190 prep with,hybridized
R6268 T21192 T21193 det a,fragment
R6269 T21193 T21191 pobj fragment,with
R6270 T21194 T21195 npadvmod DIG,labeled
R6271 T21195 T21193 amod labeled,fragment
R6272 T21196 T21195 punct -,labeled
R6273 T21197 T21198 nummod 823,bp
R6274 T21198 T21193 compound bp,fragment
R6275 T21199 T21200 compound BamHI,HindIII
R6276 T21200 T21193 compound HindIII,fragment
R6277 T21201 T21200 punct /,HindIII
R6278 T21202 T21193 acl located,fragment
R6279 T21203 T21202 advmod immediately,located
R6280 T21204 T21203 advmod upstream,immediately
R6281 T21205 T21203 prep of,immediately
R6282 T21206 T21207 det the,arm
R6283 T21207 T21205 pobj arm,of
R6284 T21208 T21207 amod short,arm
R6285 T21209 T21207 prep of,arm
R6286 T21210 T21211 det the,vector
R6287 T21211 T21209 pobj vector,of
R6288 T21212 T21211 compound targeting,vector
R6289 T21213 T21214 punct (,Figure
R6290 T21214 T21190 parataxis Figure,hybridized
R6291 T21215 T21214 nummod 1,Figure
R6292 T21216 T21214 punct ),Figure
R6293 T21217 T21177 punct .,blotted
R6294 T21219 T21220 det A,fragment
R6295 T21220 T21225 nsubj fragment,was
R6296 T21221 T21222 nummod 3.5,kb
R6297 T21222 T21220 compound kb,fragment
R6298 T21223 T21222 punct -,kb
R6299 T21224 T21220 compound BamHI,fragment
R6300 T21226 T21220 acl corresponding,fragment
R6301 T21227 T21226 prep to,corresponding
R6302 T21228 T21229 det the,allele
R6303 T21229 T21227 pobj allele,to
R6304 T21230 T21231 amod wild,type
R6305 T21231 T21229 compound type,allele
R6306 T21232 T21231 punct -,type
R6307 T21233 T21225 acomp present,was
R6308 T21234 T21225 prep in,was
R6309 T21235 T21236 det all,cells
R6310 T21236 T21234 pobj cells,in
R6311 T21237 T21225 punct ", ",was
R6312 T21238 T21239 mark whereas,detected
R6313 T21239 T21225 advcl detected,was
R6314 T21240 T21241 det a,fragment
R6315 T21241 T21239 nsubjpass fragment,detected
R6316 T21242 T21243 nummod 5.3,kb
R6317 T21243 T21241 compound kb,fragment
R6318 T21244 T21243 punct -,kb
R6319 T21245 T21241 prep from,fragment
R6320 T21246 T21247 det the,allele
R6321 T21247 T21245 pobj allele,from
R6322 T21248 T21247 compound mutant,allele
R6323 T21249 T21239 auxpass was,detected
R6324 T21250 T21239 prep in,detected
R6325 T21251 T21252 nummod two,lines
R6326 T21252 T21250 pobj lines,in
R6327 T21253 T21252 amod targeted,lines
R6328 T21254 T21252 compound cell,lines
R6329 T21255 T21239 punct ", ",detected
R6330 T21256 T21239 dep designated,detected
R6331 T21257 T21256 oprd ES27,designated
R6332 T21258 T21257 cc and,ES27
R6333 T21259 T21257 conj ES36,ES27
R6334 T21260 T21225 punct .,was
R6335 T21262 T21263 punct (,B
R6336 T21263 T21264 meta B,carried
R6337 T21265 T21263 punct ),B
R6338 T21266 T21267 compound PCR,genotyping
R6339 T21267 T21264 nsubjpass genotyping,carried
R6340 T21268 T21264 auxpass was,carried
R6341 T21269 T21264 prt out,carried
R6342 T21270 T21264 prep with,carried
R6343 T21271 T21272 nummod two,reactions
R6344 T21272 T21270 pobj reactions,with
R6345 T21273 T21272 amod separate,reactions
R6346 T21274 T21272 acl designed,reactions
R6347 T21275 T21276 aux to,amplify
R6348 T21276 T21274 advcl amplify,designed
R6349 T21277 T21278 preconj either,segment
R6350 T21278 T21276 dobj segment,amplify
R6351 T21279 T21278 det a,segment
R6352 T21280 T21281 nummod "2,748",bp
R6353 T21281 T21278 compound bp,segment
R6354 T21282 T21278 prep from,segment
R6355 T21283 T21284 det the,allele
R6356 T21284 T21282 pobj allele,from
R6357 T21285 T21286 amod wild,type
R6358 T21286 T21284 compound type,allele
R6359 T21287 T21286 punct -,type
R6360 T21288 T21278 cc or,segment
R6361 T21289 T21290 det a,segment
R6362 T21290 T21278 conj segment,segment
R6363 T21291 T21292 nummod "2,711",bp
R6364 T21292 T21290 compound bp,segment
R6365 T21293 T21290 prep from,segment
R6366 T21294 T21295 det the,allele
R6367 T21295 T21293 pobj allele,from
R6368 T21296 T21295 compound mutant,allele
R6369 T21297 T21264 punct .,carried
R6370 T21299 T21300 det Both,reactions
R6371 T21300 T21301 nsubj reactions,used
R6372 T21302 T21303 det a,primer
R6373 T21303 T21301 dobj primer,used
R6374 T21304 T21305 amod common,sense
R6375 T21305 T21303 compound sense,primer
R6376 T21306 T21303 acl located,primer
R6377 T21307 T21306 prep in,located
R6378 T21308 T21307 pobj intron,in
R6379 T21309 T21308 nummod 4,intron
R6380 T21310 T21306 punct ", ",located
R6381 T21311 T21306 prep outside,located
R6382 T21312 T21313 det the,arm
R6383 T21313 T21311 pobj arm,outside
R6384 T21314 T21313 amod short,arm
R6385 T21315 T21313 prep of,arm
R6386 T21316 T21317 det the,vector
R6387 T21317 T21315 pobj vector,of
R6388 T21318 T21317 compound targeting,vector
R6389 T21319 T21313 punct ", ",arm
R6390 T21320 T21313 cc and,arm
R6391 T21321 T21322 amod distinct,primers
R6392 T21322 T21313 conj primers,arm
R6393 T21323 T21324 npadvmod allele,specific
R6394 T21324 T21322 amod specific,primers
R6395 T21325 T21324 punct -,specific
R6396 T21326 T21322 compound antisense,primers
R6397 T21327 T21301 punct .,used
R6398 T21329 T21330 det The,reaction
R6399 T21330 T21331 nsubj reaction,used
R6400 T21332 T21333 aux to,detect
R6401 T21333 T21330 advcl detect,reaction
R6402 T21334 T21335 det the,allele
R6403 T21335 T21333 dobj allele,detect
R6404 T21336 T21337 amod wild,type
R6405 T21337 T21335 compound type,allele
R6406 T21338 T21337 punct -,type
R6407 T21339 T21340 det an,primer
R6408 T21340 T21331 dobj primer,used
R6409 T21341 T21340 compound antisense,primer
R6410 T21342 T21340 acl located,primer
R6411 T21343 T21342 prep in,located
R6412 T21344 T21343 pobj exon,in
R6413 T21345 T21344 nummod 7,exon
R6414 T21346 T21347 mark while,done
R6415 T21347 T21331 advcl done,used
R6416 T21348 T21349 det the,amplification
R6417 T21349 T21347 nsubjpass amplification,done
R6418 T21350 T21349 prep of,amplification
R6419 T21351 T21352 det the,sequence
R6420 T21352 T21350 pobj sequence,of
R6421 T21353 T21352 compound mutant,sequence
R6422 T21354 T21347 auxpass was,done
R6423 T21355 T21347 prep with,done
R6424 T21356 T21357 det an,primer
R6425 T21357 T21355 pobj primer,with
R6426 T21358 T21357 compound antisense,primer
R6427 T21359 T21357 prep in,primer
R6428 T21360 T21361 det the,cassette
R6429 T21361 T21359 pobj cassette,in
R6430 T21362 T21363 compound PGK,Neo
R6431 T21363 T21361 compound Neo,cassette
R6432 T21364 T21363 punct -,Neo
R6433 T21365 T21331 punct .,used
R6434 T21367 T21368 det The,results
R6435 T21368 T21369 nsubj results,confirm
R6436 T21370 T21371 det the,presence
R6437 T21371 T21369 dobj presence,confirm
R6438 T21372 T21371 prep of,presence
R6439 T21373 T21374 det the,allele
R6440 T21374 T21372 pobj allele,of
R6441 T21375 T21376 amod wild,type
R6442 T21376 T21374 compound type,allele
R6443 T21377 T21376 punct -,type
R6444 T21378 T21371 prep in,presence
R6445 T21379 T21380 det all,cells
R6446 T21380 T21378 pobj cells,in
R6447 T21381 T21369 punct ", ",confirm
R6448 T21382 T21383 mark whereas,observed
R6449 T21383 T21369 advcl observed,confirm
R6450 T21384 T21385 det the,signal
R6451 T21385 T21383 nsubjpass signal,observed
R6452 T21386 T21385 compound mutant,signal
R6453 T21387 T21385 compound allele,signal
R6454 T21388 T21383 auxpass was,observed
R6455 T21389 T21390 advmod only,in
R6456 T21390 T21383 prep in,observed
R6457 T21391 T21392 det the,clones
R6458 T21392 T21390 pobj clones,in
R6459 T21393 T21392 nummod two,clones
R6460 T21394 T21392 amod targeted,clones
R6461 T21395 T21369 punct .,confirm
R6462 T21397 T21398 punct (,denotes
R6463 T21399 T21398 nsubj M,denotes
R6464 T21400 T21398 punct ),denotes
R6465 T21401 T21402 det the,marker
R6466 T21402 T21398 dobj marker,denotes
R6467 T21403 T21404 amod molecular,weight
R6468 T21404 T21402 compound weight,marker
R6469 T21405 T21398 punct .,denotes