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PMC:1382200 / 39134-39658 JSONTXT 4 Projects

Annnotations TAB TSV DIC JSON TextAE

Id Subject Object Predicate Lexical cue
T20497 0-4 NN denotes Time
T20499 5-7 IN denotes of
T20500 8-21 NN denotes fertilization
T20501 22-25 VBD denotes was
T20498 26-36 VBN denotes determined
T20502 37-39 IN denotes by
T20503 40-51 NN denotes observation
T20504 52-54 IN denotes of
T20505 55-65 NN denotes copulation
T20506 66-71 NNS denotes plugs
T20507 71-73 , denotes ,
T20508 73-76 CC denotes and
T20509 77-81 NN denotes noon
T20511 82-84 IN denotes of
T20512 85-89 DT denotes that
T20513 90-93 NN denotes day
T20514 94-97 VBD denotes was
T20510 98-105 VBN denotes defined
T20515 106-108 IN denotes as
T20516 109-113 NN denotes E0.5
T20517 113-114 . denotes .
T20518 114-264 sentence denotes Pre-implantation embryos were obtained by dissecting the uteri out of pregnant females at E2.5 or E3.5 and flushing the oviducts with ES cell medium.
T20519 115-131 JJ denotes Pre-implantation
T20520 132-139 NNS denotes embryos
T20522 140-144 VBD denotes were
T20521 145-153 VBN denotes obtained
T20523 154-156 IN denotes by
T20524 157-167 VBG denotes dissecting
T20525 168-171 DT denotes the
T20526 172-177 NNS denotes uteri
T20527 178-181 IN denotes out
T20528 182-184 IN denotes of
T20529 185-193 JJ denotes pregnant
T20530 194-201 NNS denotes females
T20531 202-204 IN denotes at
T20532 205-209 NN denotes E2.5
T20533 210-212 CC denotes or
T20534 213-217 NN denotes E3.5
T20535 218-221 CC denotes and
T20536 222-230 VBG denotes flushing
T20537 231-234 DT denotes the
T20538 235-243 NNS denotes oviducts
T20539 244-248 IN denotes with
T20540 249-251 NN denotes ES
T20542 252-256 NN denotes cell
T20541 257-263 NN denotes medium
T20543 263-264 . denotes .
T20544 264-481 sentence denotes Isolated embryos were then digested for five hours at 55°C in 20 μL of lysis buffer (50 mM Tris-HCl, 0.5% Triton X-100, 200 μL/mL proteinase K, pH 8.0), followed by ten minutes at 95°C to inactivate the proteinase K.
T20545 265-273 VBN denotes Isolated
T20546 274-281 NNS denotes embryos
T20548 282-286 VBD denotes were
T20549 287-291 RB denotes then
T20547 292-300 VBN denotes digested
T20550 301-304 IN denotes for
T20551 305-309 CD denotes five
T20552 310-315 NNS denotes hours
T20553 316-318 IN denotes at
T20554 319-321 CD denotes 55
T20555 321-323 NN denotes °C
T20556 324-326 IN denotes in
T20557 327-329 CD denotes 20
T20558 330-332 NN denotes μL
T20559 333-335 IN denotes of
T20560 336-341 NN denotes lysis
T20561 342-348 NN denotes buffer
T20562 349-350 -LRB- denotes (
T20564 350-352 CD denotes 50
T20565 353-355 NN denotes mM
T20567 356-360 NN denotes Tris
T20568 360-361 HYPH denotes -
T20566 361-364 NN denotes HCl
T20569 364-366 , denotes ,
T20570 366-369 CD denotes 0.5
T20571 369-370 NN denotes %
T20573 371-377 NNP denotes Triton
T20572 378-379 NN denotes X
T20574 379-380 HYPH denotes -
T20575 380-383 CD denotes 100
T20576 383-385 , denotes ,
T20577 385-388 CD denotes 200
T20578 389-391 NN denotes μL
T20580 391-392 SYM denotes /
T20581 392-394 NN denotes mL
T20582 395-405 NN denotes proteinase
T20579 406-407 NN denotes K
T20583 407-409 , denotes ,
T20563 409-411 NN denotes pH
T20584 412-415 CD denotes 8.0
T20585 415-416 -RRB- denotes )
T20586 416-418 , denotes ,
T20587 418-426 VBN denotes followed
T20588 427-429 IN denotes by
T20589 430-433 CD denotes ten
T20590 434-441 NNS denotes minutes
T20591 442-444 IN denotes at
T20592 445-447 CD denotes 95
T20593 447-449 NN denotes °C
T20594 450-452 TO denotes to
T20595 453-463 VB denotes inactivate
T20596 464-467 DT denotes the
T20598 468-478 NN denotes proteinase
T20597 479-480 NN denotes K
T20599 480-481 . denotes .
T20600 481-524 sentence denotes Lysates were then used for PCR genotyping.
T20601 482-489 NNS denotes Lysates
T20603 490-494 VBD denotes were
T20604 495-499 RB denotes then
T20602 500-504 VBN denotes used
T20605 505-508 IN denotes for
T20606 509-512 NN denotes PCR
T20607 513-523 NN denotes genotyping
T20608 523-524 . denotes .
R6127 T20497 T20498 nsubjpass Time,determined
R6128 T20499 T20497 prep of,Time
R6129 T20500 T20499 pobj fertilization,of
R6130 T20501 T20498 auxpass was,determined
R6131 T20502 T20498 agent by,determined
R6132 T20503 T20502 pobj observation,by
R6133 T20504 T20503 prep of,observation
R6134 T20505 T20506 compound copulation,plugs
R6135 T20506 T20504 pobj plugs,of
R6136 T20507 T20498 punct ", ",determined
R6137 T20508 T20498 cc and,determined
R6138 T20509 T20510 nsubjpass noon,defined
R6139 T20510 T20498 conj defined,determined
R6140 T20511 T20509 prep of,noon
R6141 T20512 T20513 det that,day
R6142 T20513 T20511 pobj day,of
R6143 T20514 T20510 auxpass was,defined
R6144 T20515 T20510 prep as,defined
R6145 T20516 T20515 pobj E0.5,as
R6146 T20517 T20510 punct .,defined
R6147 T20519 T20520 amod Pre-implantation,embryos
R6148 T20520 T20521 nsubjpass embryos,obtained
R6149 T20522 T20521 auxpass were,obtained
R6150 T20523 T20521 prep by,obtained
R6151 T20524 T20523 pcomp dissecting,by
R6152 T20525 T20526 det the,uteri
R6153 T20526 T20524 dobj uteri,dissecting
R6154 T20527 T20524 prep out,dissecting
R6155 T20528 T20527 prep of,out
R6156 T20529 T20530 amod pregnant,females
R6157 T20530 T20528 pobj females,of
R6158 T20531 T20524 prep at,dissecting
R6159 T20532 T20531 pobj E2.5,at
R6160 T20533 T20532 cc or,E2.5
R6161 T20534 T20532 conj E3.5,E2.5
R6162 T20535 T20524 cc and,dissecting
R6163 T20536 T20524 conj flushing,dissecting
R6164 T20537 T20538 det the,oviducts
R6165 T20538 T20536 dobj oviducts,flushing
R6166 T20539 T20536 prep with,flushing
R6167 T20540 T20541 compound ES,medium
R6168 T20541 T20539 pobj medium,with
R6169 T20542 T20541 compound cell,medium
R6170 T20543 T20521 punct .,obtained
R6171 T20545 T20546 amod Isolated,embryos
R6172 T20546 T20547 nsubjpass embryos,digested
R6173 T20548 T20547 auxpass were,digested
R6174 T20549 T20547 advmod then,digested
R6175 T20550 T20547 prep for,digested
R6176 T20551 T20552 nummod five,hours
R6177 T20552 T20550 pobj hours,for
R6178 T20553 T20547 prep at,digested
R6179 T20554 T20555 nummod 55,°C
R6180 T20555 T20553 pobj °C,at
R6181 T20556 T20547 prep in,digested
R6182 T20557 T20558 nummod 20,μL
R6183 T20558 T20556 pobj μL,in
R6184 T20559 T20558 prep of,μL
R6185 T20560 T20561 compound lysis,buffer
R6186 T20561 T20559 pobj buffer,of
R6187 T20562 T20563 punct (,pH
R6188 T20563 T20561 parataxis pH,buffer
R6189 T20564 T20565 nummod 50,mM
R6190 T20565 T20566 compound mM,HCl
R6191 T20566 T20563 dep HCl,pH
R6192 T20567 T20566 compound Tris,HCl
R6193 T20568 T20566 punct -,HCl
R6194 T20569 T20563 punct ", ",pH
R6195 T20570 T20571 nummod 0.5,%
R6196 T20571 T20572 compound %,X
R6197 T20572 T20563 dep X,pH
R6198 T20573 T20572 compound Triton,X
R6199 T20574 T20572 punct -,X
R6200 T20575 T20572 nummod 100,X
R6201 T20576 T20563 punct ", ",pH
R6202 T20577 T20578 nummod 200,μL
R6203 T20578 T20579 nmod μL,K
R6204 T20579 T20563 dep K,pH
R6205 T20580 T20581 punct /,mL
R6206 T20581 T20578 prep mL,μL
R6207 T20582 T20579 compound proteinase,K
R6208 T20583 T20563 punct ", ",pH
R6209 T20584 T20563 nummod 8.0,pH
R6210 T20585 T20563 punct ),pH
R6211 T20586 T20561 punct ", ",buffer
R6212 T20587 T20561 acl followed,buffer
R6213 T20588 T20587 agent by,followed
R6214 T20589 T20590 nummod ten,minutes
R6215 T20590 T20588 pobj minutes,by
R6216 T20591 T20590 prep at,minutes
R6217 T20592 T20593 nummod 95,°C
R6218 T20593 T20591 pobj °C,at
R6219 T20594 T20595 aux to,inactivate
R6220 T20595 T20587 advcl inactivate,followed
R6221 T20596 T20597 det the,K
R6222 T20597 T20595 dobj K,inactivate
R6223 T20598 T20597 compound proteinase,K
R6224 T20599 T20547 punct .,digested
R6225 T20601 T20602 nsubjpass Lysates,used
R6226 T20603 T20602 auxpass were,used
R6227 T20604 T20602 advmod then,used
R6228 T20605 T20602 prep for,used
R6229 T20606 T20607 compound PCR,genotyping
R6230 T20607 T20605 pobj genotyping,for
R6231 T20608 T20602 punct .,used