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PMC:1382200 / 37044-37473 JSONTXT 4 Projects

Annnotations TAB TSV DIC JSON TextAE

Id Subject Object Predicate Lexical cue
T19820 0-3 IN denotes Due
T19819 0-173 sentence denotes Due to the limited amount of genetic material available in pre-implantation embryos, a nested PCR strategy was developed to yield reliable genotyping information (Figure 4).
T19822 4-6 IN denotes to
T19823 7-10 DT denotes the
T19825 11-18 VBN denotes limited
T19824 19-25 NN denotes amount
T19826 26-28 IN denotes of
T19827 29-36 JJ denotes genetic
T19828 37-45 NN denotes material
T19829 46-55 JJ denotes available
T19830 56-58 IN denotes in
T19831 59-75 JJ denotes pre-implantation
T19832 76-83 NNS denotes embryos
T19833 83-85 , denotes ,
T19834 85-86 DT denotes a
T19836 87-93 VBN denotes nested
T19837 94-97 NN denotes PCR
T19835 98-106 NN denotes strategy
T19838 107-110 VBD denotes was
T19821 111-120 VBN denotes developed
T19839 121-123 TO denotes to
T19840 124-129 VB denotes yield
T19841 130-138 JJ denotes reliable
T19843 139-149 NN denotes genotyping
T19842 150-161 NN denotes information
T19844 162-163 -LRB- denotes (
T19845 163-169 NN denotes Figure
T19846 170-171 CD denotes 4
T19847 171-172 -RRB- denotes )
T19848 172-173 . denotes .
T19849 173-252 sentence denotes Whole embryos were first digested in 20 μL of proteinase K buffer (see below).
T19850 174-179 JJ denotes Whole
T19851 180-187 NNS denotes embryos
T19853 188-192 VBD denotes were
T19854 193-198 RB denotes first
T19852 199-207 VBN denotes digested
T19855 208-210 IN denotes in
T19856 211-213 CD denotes 20
T19857 214-216 NN denotes μL
T19858 217-219 IN denotes of
T19859 220-230 NN denotes proteinase
T19860 231-232 NN denotes K
T19861 233-239 NN denotes buffer
T19862 240-241 -LRB- denotes (
T19863 241-244 VB denotes see
T19864 245-250 RB denotes below
T19865 250-251 -RRB- denotes )
T19866 251-252 . denotes .
T19867 252-429 sentence denotes The lysate was then divided in two, with half (10 μL) being used in the amplification of the wild-type allele and the remaining 10 μL in the amplification of the mutant allele.
T19868 253-256 DT denotes The
T19869 257-263 NN denotes lysate
T19871 264-267 VBD denotes was
T19872 268-272 RB denotes then
T19870 273-280 VBN denotes divided
T19873 281-283 IN denotes in
T19874 284-287 CD denotes two
T19875 287-289 , denotes ,
T19876 289-293 IN denotes with
T19877 294-298 NN denotes half
T19879 299-300 -LRB- denotes (
T19881 300-302 CD denotes 10
T19880 303-305 NN denotes μL
T19882 305-306 -RRB- denotes )
T19883 307-312 VBG denotes being
T19878 313-317 VBN denotes used
T19884 318-320 IN denotes in
T19885 321-324 DT denotes the
T19886 325-338 NN denotes amplification
T19887 339-341 IN denotes of
T19888 342-345 DT denotes the
T19890 346-350 JJ denotes wild
T19892 350-351 HYPH denotes -
T19891 351-355 NN denotes type
T19889 356-362 NN denotes allele
T19893 363-366 CC denotes and
T19894 367-370 DT denotes the
T19896 371-380 VBG denotes remaining
T19897 381-383 CD denotes 10
T19895 384-386 NN denotes μL
T19898 387-389 IN denotes in
T19899 390-393 DT denotes the
T19900 394-407 NN denotes amplification
T19901 408-410 IN denotes of
T19902 411-414 DT denotes the
T19904 415-421 NN denotes mutant
T19903 422-428 NN denotes allele
T19905 428-429 . denotes .
R5738 T19820 T19821 prep Due,developed
R5739 T19822 T19820 pcomp to,Due
R5740 T19823 T19824 det the,amount
R5741 T19824 T19820 pobj amount,Due
R5742 T19825 T19824 amod limited,amount
R5743 T19826 T19824 prep of,amount
R5744 T19827 T19828 amod genetic,material
R5745 T19828 T19826 pobj material,of
R5746 T19829 T19828 amod available,material
R5747 T19830 T19824 prep in,amount
R5748 T19831 T19832 amod pre-implantation,embryos
R5749 T19832 T19830 pobj embryos,in
R5750 T19833 T19821 punct ", ",developed
R5751 T19834 T19835 det a,strategy
R5752 T19835 T19821 nsubjpass strategy,developed
R5753 T19836 T19835 amod nested,strategy
R5754 T19837 T19835 compound PCR,strategy
R5755 T19838 T19821 auxpass was,developed
R5756 T19839 T19840 aux to,yield
R5757 T19840 T19821 advcl yield,developed
R5758 T19841 T19842 amod reliable,information
R5759 T19842 T19840 dobj information,yield
R5760 T19843 T19842 compound genotyping,information
R5761 T19844 T19845 punct (,Figure
R5762 T19845 T19821 parataxis Figure,developed
R5763 T19846 T19845 nummod 4,Figure
R5764 T19847 T19845 punct ),Figure
R5765 T19848 T19821 punct .,developed
R5766 T19850 T19851 amod Whole,embryos
R5767 T19851 T19852 nsubjpass embryos,digested
R5768 T19853 T19852 auxpass were,digested
R5769 T19854 T19852 advmod first,digested
R5770 T19855 T19852 prep in,digested
R5771 T19856 T19857 nummod 20,μL
R5772 T19857 T19855 pobj μL,in
R5773 T19858 T19857 prep of,μL
R5774 T19859 T19860 compound proteinase,K
R5775 T19860 T19861 compound K,buffer
R5776 T19861 T19858 pobj buffer,of
R5777 T19862 T19863 punct (,see
R5778 T19863 T19852 parataxis see,digested
R5779 T19864 T19863 advmod below,see
R5780 T19865 T19863 punct ),see
R5781 T19866 T19852 punct .,digested
R5782 T19868 T19869 det The,lysate
R5783 T19869 T19870 nsubjpass lysate,divided
R5784 T19871 T19870 auxpass was,divided
R5785 T19872 T19870 advmod then,divided
R5786 T19873 T19870 prep in,divided
R5787 T19874 T19873 pobj two,in
R5788 T19875 T19870 punct ", ",divided
R5789 T19876 T19870 prep with,divided
R5790 T19877 T19878 nsubjpass half,used
R5791 T19878 T19876 pcomp used,with
R5792 T19879 T19880 punct (,μL
R5793 T19880 T19877 parataxis μL,half
R5794 T19881 T19880 nummod 10,μL
R5795 T19882 T19880 punct ),μL
R5796 T19883 T19878 auxpass being,used
R5797 T19884 T19878 prep in,used
R5798 T19885 T19886 det the,amplification
R5799 T19886 T19884 pobj amplification,in
R5800 T19887 T19886 prep of,amplification
R5801 T19888 T19889 det the,allele
R5802 T19889 T19887 pobj allele,of
R5803 T19890 T19891 amod wild,type
R5804 T19891 T19889 compound type,allele
R5805 T19892 T19891 punct -,type
R5806 T19893 T19878 cc and,used
R5807 T19894 T19895 det the,μL
R5808 T19895 T19878 conj μL,used
R5809 T19896 T19895 amod remaining,μL
R5810 T19897 T19895 nummod 10,μL
R5811 T19898 T19895 prep in,μL
R5812 T19899 T19900 det the,amplification
R5813 T19900 T19898 pobj amplification,in
R5814 T19901 T19900 prep of,amplification
R5815 T19902 T19903 det the,allele
R5816 T19903 T19901 pobj allele,of
R5817 T19904 T19903 compound mutant,allele
R5818 T19905 T19870 punct .,divided