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PMC:1382200 / 18142-21685 JSONTXT 4 Projects

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Id Subject Object Predicate Lexical cue
T13129 0-2 PRP denotes We
T13130 3-9 VBP denotes report
T13131 10-14 RB denotes here
T13132 15-19 IN denotes that
T13134 20-25 NN denotes Capn2
T13136 25-26 SYM denotes -
T13137 26-27 HYPH denotes /
T13138 27-28 SYM denotes -
T13135 29-33 NNS denotes mice
T13139 33-35 , denotes ,
T13140 35-40 WDT denotes which
T13141 41-45 VBP denotes lack
T13142 46-49 DT denotes the
T13144 50-51 NN denotes m
T13146 51-52 HYPH denotes -
T13145 52-59 NN denotes calpain
T13147 60-69 JJ denotes catalytic
T13143 70-77 NN denotes subunit
T13148 77-79 , denotes ,
T13133 79-82 VBP denotes die
T13149 83-85 IN denotes at
T13150 86-89 DT denotes the
T13152 90-105 NN denotes preimplantation
T13151 106-111 NN denotes stage
T13153 112-114 IN denotes of
T13154 115-126 NN denotes development
T13155 126-127 . denotes .
T13156 127-232 sentence denotes This observation allows us to now conclude that m-calpain is specifically required during embryogenesis.
T13157 128-132 DT denotes This
T13158 133-144 NN denotes observation
T13159 145-151 VBZ denotes allows
T13160 152-154 PRP denotes us
T13162 155-157 TO denotes to
T13163 158-161 RB denotes now
T13161 162-170 VB denotes conclude
T13164 171-175 IN denotes that
T13166 176-177 NN denotes m
T13168 177-178 HYPH denotes -
T13167 178-185 NN denotes calpain
T13169 186-188 VBZ denotes is
T13170 189-201 RB denotes specifically
T13165 202-210 VBN denotes required
T13171 211-217 IN denotes during
T13172 218-231 NN denotes embryogenesis
T13173 231-232 . denotes .
T13174 232-498 sentence denotes Since homozygous disruption of the Capn4 gene was also expected to abolish m-calpain activity, this result is in agreement with the phenotype presented by one of the two Capn4 targeted lines in which preimplantation lethality of null embryos was also observed [32].
T13175 233-238 IN denotes Since
T13177 239-249 JJ denotes homozygous
T13178 250-260 NN denotes disruption
T13179 261-263 IN denotes of
T13180 264-267 DT denotes the
T13182 268-273 NN denotes Capn4
T13181 274-278 NN denotes gene
T13183 279-282 VBD denotes was
T13184 283-287 RB denotes also
T13176 288-296 VBN denotes expected
T13186 297-299 TO denotes to
T13187 300-307 VB denotes abolish
T13188 308-309 NN denotes m
T13190 309-310 HYPH denotes -
T13189 310-317 NN denotes calpain
T13191 318-326 NN denotes activity
T13192 326-328 , denotes ,
T13193 328-332 DT denotes this
T13194 333-339 NN denotes result
T13185 340-342 VBZ denotes is
T13195 343-345 IN denotes in
T13196 346-355 NN denotes agreement
T13197 356-360 IN denotes with
T13198 361-364 DT denotes the
T13199 365-374 NN denotes phenotype
T13200 375-384 VBN denotes presented
T13201 385-387 IN denotes by
T13202 388-391 CD denotes one
T13203 392-394 IN denotes of
T13204 395-398 DT denotes the
T13206 399-402 CD denotes two
T13207 403-408 NN denotes Capn4
T13208 409-417 VBN denotes targeted
T13205 418-423 NNS denotes lines
T13209 424-426 IN denotes in
T13211 427-432 WDT denotes which
T13212 433-448 NN denotes preimplantation
T13213 449-458 NN denotes lethality
T13214 459-461 IN denotes of
T13215 462-466 JJ denotes null
T13216 467-474 NNS denotes embryos
T13217 475-478 VBD denotes was
T13218 479-483 RB denotes also
T13210 484-492 VBN denotes observed
T13219 493-494 -LRB- denotes [
T13220 494-496 CD denotes 32
T13221 496-497 -RRB- denotes ]
T13222 497-498 . denotes .
T13223 498-651 sentence denotes The survival of Capn4-/- embryos from the original targeted line reported by Arthur and colleagues to mid-gestation is more difficult to reconcile [31].
T13224 499-502 DT denotes The
T13225 503-511 NN denotes survival
T13227 512-514 IN denotes of
T13228 515-520 NN denotes Capn4
T13230 520-521 SYM denotes -
T13231 521-522 HYPH denotes /
T13232 522-523 SYM denotes -
T13229 524-531 NNS denotes embryos
T13233 532-536 IN denotes from
T13234 537-540 DT denotes the
T13236 541-549 JJ denotes original
T13237 550-558 VBN denotes targeted
T13235 559-563 NN denotes line
T13238 564-572 VBN denotes reported
T13239 573-575 IN denotes by
T13240 576-582 NNP denotes Arthur
T13241 583-586 CC denotes and
T13242 587-597 NNS denotes colleagues
T13243 598-600 IN denotes to
T13244 601-614 NN denotes mid-gestation
T13226 615-617 VBZ denotes is
T13245 618-622 RBR denotes more
T13246 623-632 JJ denotes difficult
T13247 633-635 TO denotes to
T13248 636-645 VB denotes reconcile
T13249 646-647 -LRB- denotes [
T13250 647-649 CD denotes 31
T13251 649-650 -RRB- denotes ]
T13252 650-651 . denotes .
T13253 651-760 sentence denotes In retrospect, it seems likely that the latter line represents a hypomorphic state, rather than a true null.
T13254 652-654 IN denotes In
T13256 655-665 NN denotes retrospect
T13257 665-667 , denotes ,
T13258 667-669 PRP denotes it
T13255 670-675 VBZ denotes seems
T13259 676-682 JJ denotes likely
T13260 683-687 IN denotes that
T13262 688-691 DT denotes the
T13264 692-698 JJ denotes latter
T13263 699-703 NN denotes line
T13261 704-714 VBZ denotes represents
T13265 715-716 DT denotes a
T13267 717-728 JJ denotes hypomorphic
T13266 729-734 NN denotes state
T13268 734-736 , denotes ,
T13269 736-742 RB denotes rather
T13270 743-747 IN denotes than
T13271 748-749 DT denotes a
T13273 750-754 JJ denotes true
T13272 755-759 NN denotes null
T13274 759-760 . denotes .
T13275 760-996 sentence denotes The Capn4 targeting strategy employed by Arthur and colleagues involved disrupting the C-terminus of the calpain small subunit by insertion of the PGK-Neo cassette into the middle of exon 9, which caused truncation of the protein [31].
T13276 761-764 DT denotes The
T13278 765-770 NN denotes Capn4
T13279 771-780 NN denotes targeting
T13277 781-789 NN denotes strategy
T13281 790-798 VBN denotes employed
T13282 799-801 IN denotes by
T13283 802-808 NNP denotes Arthur
T13284 809-812 CC denotes and
T13285 813-823 NNS denotes colleagues
T13280 824-832 VBN denotes involved
T13286 833-843 VBG denotes disrupting
T13287 844-847 DT denotes the
T13289 848-849 NN denotes C
T13290 849-850 HYPH denotes -
T13288 850-858 NN denotes terminus
T13291 859-861 IN denotes of
T13292 862-865 DT denotes the
T13294 866-873 NN denotes calpain
T13295 874-879 JJ denotes small
T13293 880-887 NN denotes subunit
T13296 888-890 IN denotes by
T13297 891-900 NN denotes insertion
T13298 901-903 IN denotes of
T13299 904-907 DT denotes the
T13301 908-911 NN denotes PGK
T13303 911-912 HYPH denotes -
T13302 912-915 NN denotes Neo
T13300 916-924 NN denotes cassette
T13304 925-929 IN denotes into
T13305 930-933 DT denotes the
T13306 934-940 NN denotes middle
T13307 941-943 IN denotes of
T13308 944-948 NN denotes exon
T13309 949-950 CD denotes 9
T13310 950-952 , denotes ,
T13311 952-957 WDT denotes which
T13312 958-964 VBD denotes caused
T13313 965-975 NN denotes truncation
T13314 976-978 IN denotes of
T13315 979-982 DT denotes the
T13316 983-990 NN denotes protein
T13317 991-992 -LRB- denotes [
T13318 992-994 CD denotes 31
T13319 994-995 -RRB- denotes ]
T13320 995-996 . denotes .
T13321 996-1240 sentence denotes This strategy was based upon previous structure/function studies showing that excision of the C-terminal 25 amino acid residues of the small subunit abolished calpain activity when co-expressed with the m-calpain large subunit in E. coli [34].
T13322 997-1001 DT denotes This
T13323 1002-1010 NN denotes strategy
T13325 1011-1014 VBD denotes was
T13324 1015-1020 VBN denotes based
T13326 1021-1025 IN denotes upon
T13327 1026-1034 JJ denotes previous
T13329 1035-1044 NN denotes structure
T13331 1044-1045 HYPH denotes /
T13330 1045-1053 NN denotes function
T13328 1054-1061 NNS denotes studies
T13332 1062-1069 VBG denotes showing
T13333 1070-1074 IN denotes that
T13335 1075-1083 NN denotes excision
T13336 1084-1086 IN denotes of
T13337 1087-1090 DT denotes the
T13339 1091-1092 NN denotes C
T13341 1092-1093 HYPH denotes -
T13340 1093-1101 JJ denotes terminal
T13342 1102-1104 CD denotes 25
T13343 1105-1110 NN denotes amino
T13338 1111-1115 NN denotes acid
T13344 1116-1124 VBZ denotes residues
T13345 1125-1127 IN denotes of
T13346 1128-1131 DT denotes the
T13348 1132-1137 JJ denotes small
T13347 1138-1145 NN denotes subunit
T13334 1146-1155 VBD denotes abolished
T13349 1156-1163 NN denotes calpain
T13350 1164-1172 NN denotes activity
T13351 1173-1177 WRB denotes when
T13352 1178-1190 VBN denotes co-expressed
T13353 1191-1195 IN denotes with
T13354 1196-1199 DT denotes the
T13356 1200-1201 NN denotes m
T13358 1201-1202 HYPH denotes -
T13357 1202-1209 NN denotes calpain
T13359 1210-1215 JJ denotes large
T13355 1216-1223 NN denotes subunit
T13360 1224-1226 IN denotes in
T13361 1227-1229 NNP denotes E.
T13362 1230-1234 NNP denotes coli
T13363 1235-1236 -LRB- denotes [
T13364 1236-1238 CD denotes 34
T13365 1238-1239 -RRB- denotes ]
T13366 1239-1240 . denotes .
T13367 1240-1386 sentence denotes In contrast, the Capn4 targeting strategy employed by Zimmerman and colleagues involved a much more extensive deletion of exons 4 through 8 [32].
T13368 1241-1243 IN denotes In
T13370 1244-1252 NN denotes contrast
T13371 1252-1254 , denotes ,
T13372 1254-1257 DT denotes the
T13374 1258-1263 NN denotes Capn4
T13375 1264-1273 VBG denotes targeting
T13373 1274-1282 NN denotes strategy
T13376 1283-1291 VBN denotes employed
T13377 1292-1294 IN denotes by
T13378 1295-1304 NNP denotes Zimmerman
T13379 1305-1308 CC denotes and
T13380 1309-1319 NNS denotes colleagues
T13369 1320-1328 VBD denotes involved
T13381 1329-1330 DT denotes a
T13383 1331-1335 RB denotes much
T13384 1336-1340 RBR denotes more
T13385 1341-1350 JJ denotes extensive
T13382 1351-1359 NN denotes deletion
T13386 1360-1362 IN denotes of
T13387 1363-1368 NNS denotes exons
T13388 1369-1370 CD denotes 4
T13389 1371-1378 IN denotes through
T13390 1379-1380 CD denotes 8
T13391 1381-1382 -LRB- denotes [
T13392 1382-1384 CD denotes 32
T13393 1384-1385 -RRB- denotes ]
T13394 1385-1386 . denotes .
T13395 1386-1540 sentence denotes It now seems probable that the difference in the time of lethality of these two targeted Capn4 lines can be explained by different extents of disruption.
T13396 1387-1389 PRP denotes It
T13398 1390-1393 RB denotes now
T13397 1394-1399 VBZ denotes seems
T13399 1400-1408 JJ denotes probable
T13400 1409-1413 IN denotes that
T13402 1414-1417 DT denotes the
T13403 1418-1428 NN denotes difference
T13404 1429-1431 IN denotes in
T13405 1432-1435 DT denotes the
T13406 1436-1440 NN denotes time
T13407 1441-1443 IN denotes of
T13408 1444-1453 NN denotes lethality
T13409 1454-1456 IN denotes of
T13410 1457-1462 DT denotes these
T13412 1463-1466 CD denotes two
T13413 1467-1475 VBN denotes targeted
T13414 1476-1481 NN denotes Capn4
T13411 1482-1487 NNS denotes lines
T13415 1488-1491 MD denotes can
T13416 1492-1494 VB denotes be
T13401 1495-1504 VBN denotes explained
T13417 1505-1507 IN denotes by
T13418 1508-1517 JJ denotes different
T13419 1518-1525 NNS denotes extents
T13420 1526-1528 IN denotes of
T13421 1529-1539 NN denotes disruption
T13422 1539-1540 . denotes .
T13423 1540-1660 sentence denotes The Zimmerman allele probably represents a true null genotype while the Arthur allele is likely a hypomorphic mutation.
T13424 1541-1544 DT denotes The
T13426 1545-1554 NNP denotes Zimmerman
T13425 1555-1561 NN denotes allele
T13428 1562-1570 RB denotes probably
T13427 1571-1581 VBZ denotes represents
T13429 1582-1583 DT denotes a
T13431 1584-1588 JJ denotes true
T13432 1589-1593 JJ denotes null
T13430 1594-1602 NN denotes genotype
T13433 1603-1608 IN denotes while
T13435 1609-1612 DT denotes the
T13437 1613-1619 NNP denotes Arthur
T13436 1620-1626 NN denotes allele
T13434 1627-1629 VBZ denotes is
T13438 1630-1636 RB denotes likely
T13439 1637-1638 DT denotes a
T13441 1639-1650 JJ denotes hypomorphic
T13440 1651-1659 NN denotes mutation
T13442 1659-1660 . denotes .
T13443 1660-1765 sentence denotes Alternate targeting strategies have been shown to yield different phenotypes in gene disruption studies.
T13444 1661-1670 JJ denotes Alternate
T13446 1671-1680 NN denotes targeting
T13445 1681-1691 NNS denotes strategies
T13448 1692-1696 VBP denotes have
T13449 1697-1701 VBN denotes been
T13447 1702-1707 VBN denotes shown
T13450 1708-1710 TO denotes to
T13451 1711-1716 VB denotes yield
T13452 1717-1726 JJ denotes different
T13453 1727-1737 NNS denotes phenotypes
T13454 1738-1740 IN denotes in
T13455 1741-1745 NN denotes gene
T13456 1746-1756 NN denotes disruption
T13457 1757-1764 NNS denotes studies
T13458 1764-1765 . denotes .
T13459 1765-1889 sentence denotes For example, three different targeting strategies were independently used to disrupt the mixed lineage leukemia (Mll) gene.
T13460 1766-1769 IN denotes For
T13462 1770-1777 NN denotes example
T13463 1777-1779 , denotes ,
T13464 1779-1784 CD denotes three
T13466 1785-1794 JJ denotes different
T13467 1795-1804 NN denotes targeting
T13465 1805-1815 NNS denotes strategies
T13468 1816-1820 VBD denotes were
T13469 1821-1834 RB denotes independently
T13461 1835-1839 VBN denotes used
T13470 1840-1842 TO denotes to
T13471 1843-1850 VB denotes disrupt
T13472 1851-1854 DT denotes the
T13474 1855-1860 JJ denotes mixed
T13476 1861-1868 NN denotes lineage
T13475 1869-1877 NN denotes leukemia
T13477 1878-1879 -LRB- denotes (
T13478 1879-1882 NN denotes Mll
T13479 1882-1883 -RRB- denotes )
T13473 1884-1888 NN denotes gene
T13480 1888-1889 . denotes .
T13481 1889-2017 sentence denotes In all three studies, homozygous null embryos perished during embryogenesis, but at different stages (E0.5, E10.5, E14.5) [35].
T13482 1890-1892 IN denotes In
T13484 1893-1896 DT denotes all
T13486 1897-1902 CD denotes three
T13485 1903-1910 NNS denotes studies
T13487 1910-1912 , denotes ,
T13488 1912-1922 JJ denotes homozygous
T13489 1923-1927 JJ denotes null
T13490 1928-1935 NNS denotes embryos
T13483 1936-1944 VBN denotes perished
T13491 1945-1951 IN denotes during
T13492 1952-1965 NN denotes embryogenesis
T13493 1965-1967 , denotes ,
T13494 1967-1970 CC denotes but
T13495 1971-1973 IN denotes at
T13496 1974-1983 JJ denotes different
T13497 1984-1990 NNS denotes stages
T13498 1991-1992 -LRB- denotes (
T13500 1992-1996 NN denotes E0.5
T13501 1996-1998 , denotes ,
T13502 1998-2003 NN denotes E10.5
T13503 2003-2005 , denotes ,
T13499 2005-2010 NN denotes E14.5
T13504 2010-2011 -RRB- denotes )
T13505 2012-2013 -LRB- denotes [
T13506 2013-2015 CD denotes 35
T13507 2015-2016 -RRB- denotes ]
T13508 2016-2017 . denotes .
T13509 2017-2160 sentence denotes The variation in phenotype was attributed to the differences in degree of function of the truncated proteins produced from the mutant alleles.
T13510 2018-2021 DT denotes The
T13511 2022-2031 NN denotes variation
T13513 2032-2034 IN denotes in
T13514 2035-2044 NN denotes phenotype
T13515 2045-2048 VBD denotes was
T13512 2049-2059 VBN denotes attributed
T13516 2060-2062 IN denotes to
T13517 2063-2066 DT denotes the
T13518 2067-2078 NNS denotes differences
T13519 2079-2081 IN denotes in
T13520 2082-2088 NN denotes degree
T13521 2089-2091 IN denotes of
T13522 2092-2100 NN denotes function
T13523 2101-2103 IN denotes of
T13524 2104-2107 DT denotes the
T13526 2108-2117 VBN denotes truncated
T13525 2118-2126 NN denotes proteins
T13527 2127-2135 VBN denotes produced
T13528 2136-2140 IN denotes from
T13529 2141-2144 DT denotes the
T13531 2145-2151 NN denotes mutant
T13530 2152-2159 NNS denotes alleles
T13532 2159-2160 . denotes .
T13533 2160-2229 sentence denotes A similar effect might be at work in the two Capn4 transgenic lines.
T13534 2161-2162 DT denotes A
T13536 2163-2170 JJ denotes similar
T13535 2171-2177 NN denotes effect
T13538 2178-2183 MD denotes might
T13537 2184-2186 VB denotes be
T13539 2187-2189 IN denotes at
T13540 2190-2194 NN denotes work
T13541 2195-2197 IN denotes in
T13542 2198-2201 DT denotes the
T13544 2202-2205 CD denotes two
T13545 2206-2211 NN denotes Capn4
T13546 2212-2222 JJ denotes transgenic
T13543 2223-2228 NNS denotes lines
T13547 2228-2229 . denotes .
T13548 2229-2329 sentence denotes Efforts were made to detect transcripts or calpain activities derived from the Arthur Capn4 allele.
T13549 2230-2237 NNS denotes Efforts
T13551 2238-2242 VBD denotes were
T13550 2243-2247 VBN denotes made
T13552 2248-2250 TO denotes to
T13553 2251-2257 VB denotes detect
T13554 2258-2269 NNS denotes transcripts
T13555 2270-2272 CC denotes or
T13556 2273-2280 NN denotes calpain
T13557 2281-2291 NNS denotes activities
T13558 2292-2299 VBN denotes derived
T13559 2300-2304 IN denotes from
T13560 2305-2308 DT denotes the
T13562 2309-2315 NNP denotes Arthur
T13563 2316-2321 NN denotes Capn4
T13561 2322-2328 NN denotes allele
T13564 2328-2329 . denotes .
T13565 2329-2527 sentence denotes This allele gave rise to multiple mRNA species, detectable by RT-PCR, reading through from the first half of exon 9 to at least two different cryptic splice sites in the PGK promoter sequence [31].
T13566 2330-2334 DT denotes This
T13567 2335-2341 NN denotes allele
T13568 2342-2346 VBD denotes gave
T13569 2347-2351 NN denotes rise
T13570 2352-2354 IN denotes to
T13571 2355-2363 JJ denotes multiple
T13573 2364-2368 NN denotes mRNA
T13572 2369-2376 NNS denotes species
T13574 2376-2378 , denotes ,
T13575 2378-2388 JJ denotes detectable
T13576 2389-2391 IN denotes by
T13577 2392-2394 NN denotes RT
T13579 2394-2395 HYPH denotes -
T13578 2395-2398 NN denotes PCR
T13580 2398-2400 , denotes ,
T13581 2400-2407 VBG denotes reading
T13582 2408-2415 RB denotes through
T13583 2416-2420 IN denotes from
T13584 2421-2424 DT denotes the
T13586 2425-2430 JJ denotes first
T13585 2431-2435 NN denotes half
T13587 2436-2438 IN denotes of
T13588 2439-2443 NN denotes exon
T13589 2444-2445 CD denotes 9
T13590 2446-2448 IN denotes to
T13591 2449-2451 RB denotes at
T13593 2452-2457 RBS denotes least
T13592 2458-2461 CD denotes two
T13595 2462-2471 JJ denotes different
T13596 2472-2479 JJ denotes cryptic
T13597 2480-2486 NN denotes splice
T13594 2487-2492 NNS denotes sites
T13598 2493-2495 IN denotes in
T13599 2496-2499 DT denotes the
T13601 2500-2503 NN denotes PGK
T13602 2504-2512 NN denotes promoter
T13600 2513-2521 NN denotes sequence
T13603 2522-2523 -LRB- denotes [
T13604 2523-2525 CD denotes 31
T13605 2525-2526 -RRB- denotes ]
T13606 2526-2527 . denotes .
T13607 2527-2710 sentence denotes These transcripts could give rise to defective calpain small subunits with 10–30 inappropriate C-terminal acids, which might be sufficient to support a low level of calpain activity.
T13608 2528-2533 DT denotes These
T13609 2534-2545 NNS denotes transcripts
T13611 2546-2551 MD denotes could
T13610 2552-2556 VB denotes give
T13612 2557-2561 NN denotes rise
T13613 2562-2564 IN denotes to
T13614 2565-2574 JJ denotes defective
T13616 2575-2582 NN denotes calpain
T13617 2583-2588 JJ denotes small
T13615 2589-2597 NNS denotes subunits
T13618 2598-2602 IN denotes with
T13619 2603-2605 CD denotes 10
T13621 2605-2606 SYM denotes –
T13620 2606-2608 CD denotes 30
T13623 2609-2622 JJ denotes inappropriate
T13624 2623-2624 NN denotes C
T13626 2624-2625 HYPH denotes -
T13625 2625-2633 JJ denotes terminal
T13622 2634-2639 NNS denotes acids
T13627 2639-2641 , denotes ,
T13628 2641-2646 WDT denotes which
T13630 2647-2652 MD denotes might
T13629 2653-2655 VB denotes be
T13631 2656-2666 JJ denotes sufficient
T13632 2667-2669 TO denotes to
T13633 2670-2677 VB denotes support
T13634 2678-2679 DT denotes a
T13636 2680-2683 JJ denotes low
T13635 2684-2689 NN denotes level
T13637 2690-2692 IN denotes of
T13638 2693-2700 NN denotes calpain
T13639 2701-2709 NN denotes activity
T13640 2709-2710 . denotes .
T13641 2710-3291 sentence denotes However, expression of calpains with these modified small subunits did not give rise to any detectable calpain activity when expressed in E. coli, although their functionality in mammalian cells has yet to be determined (J.S. Elce, unpublished work) It has also been suggested that calpain large subunits alone might provide some activity in eukaryotic cells, although the Zimmerman et al. knockout appears to exclude that possibility, and no calpain activity was observed in our hands when calpain large subunits were expressed alone either in E. coli or in mammalian cells [36].
T13642 2711-2718 RB denotes However
T13644 2718-2720 , denotes ,
T13645 2720-2730 NN denotes expression
T13646 2731-2733 IN denotes of
T13647 2734-2742 NNS denotes calpains
T13648 2743-2747 IN denotes with
T13649 2748-2753 DT denotes these
T13651 2754-2762 VBN denotes modified
T13652 2763-2768 JJ denotes small
T13650 2769-2777 NNS denotes subunits
T13653 2778-2781 VBD denotes did
T13654 2782-2785 RB denotes not
T13643 2786-2790 VB denotes give
T13656 2791-2795 NN denotes rise
T13657 2796-2798 IN denotes to
T13658 2799-2802 DT denotes any
T13660 2803-2813 JJ denotes detectable
T13661 2814-2821 NN denotes calpain
T13659 2822-2830 NN denotes activity
T13662 2831-2835 WRB denotes when
T13663 2836-2845 VBN denotes expressed
T13664 2846-2848 IN denotes in
T13665 2849-2851 NNP denotes E.
T13666 2852-2856 NNP denotes coli
T13667 2856-2858 , denotes ,
T13668 2858-2866 IN denotes although
T13670 2867-2872 PRP$ denotes their
T13671 2873-2886 NN denotes functionality
T13672 2887-2889 IN denotes in
T13673 2890-2899 JJ denotes mammalian
T13674 2900-2905 NNS denotes cells
T13669 2906-2909 VBZ denotes has
T13675 2910-2913 RB denotes yet
T13676 2914-2916 TO denotes to
T13678 2917-2919 VB denotes be
T13677 2920-2930 VBN denotes determined
T13679 2931-2932 -LRB- denotes (
T13680 2932-2936 NNP denotes J.S.
T13681 2937-2941 NNP denotes Elce
T13682 2941-2943 , denotes ,
T13683 2943-2954 JJ denotes unpublished
T13684 2955-2959 NN denotes work
T13685 2959-2960 -RRB- denotes )
T13686 2961-2963 PRP denotes It
T13687 2964-2967 VBZ denotes has
T13688 2968-2972 RB denotes also
T13689 2973-2977 VBN denotes been
T13655 2978-2987 VBN denotes suggested
T13690 2988-2992 IN denotes that
T13692 2993-3000 NN denotes calpain
T13694 3001-3006 JJ denotes large
T13693 3007-3015 NNS denotes subunits
T13695 3016-3021 RB denotes alone
T13696 3022-3027 MD denotes might
T13691 3028-3035 VB denotes provide
T13697 3036-3040 DT denotes some
T13698 3041-3049 NN denotes activity
T13699 3050-3052 IN denotes in
T13700 3053-3063 JJ denotes eukaryotic
T13701 3064-3069 NNS denotes cells
T13702 3069-3071 , denotes ,
T13703 3071-3079 IN denotes although
T13705 3080-3083 DT denotes the
T13707 3084-3093 NNP denotes Zimmerman
T13708 3094-3096 FW denotes et
T13709 3097-3100 FW denotes al.
T13706 3101-3109 NN denotes knockout
T13704 3110-3117 VBZ denotes appears
T13710 3118-3120 TO denotes to
T13711 3121-3128 VB denotes exclude
T13712 3129-3133 DT denotes that
T13713 3134-3145 NN denotes possibility
T13714 3145-3147 , denotes ,
T13715 3147-3150 CC denotes and
T13716 3151-3153 DT denotes no
T13718 3154-3161 NN denotes calpain
T13717 3162-3170 NN denotes activity
T13720 3171-3174 VBD denotes was
T13719 3175-3183 VBN denotes observed
T13721 3184-3186 IN denotes in
T13722 3187-3190 PRP$ denotes our
T13723 3191-3196 NNS denotes hands
T13724 3197-3201 WRB denotes when
T13726 3202-3209 NN denotes calpain
T13728 3210-3215 JJ denotes large
T13727 3216-3224 NNS denotes subunits
T13729 3225-3229 VBD denotes were
T13725 3230-3239 VBN denotes expressed
T13730 3240-3245 RB denotes alone
T13731 3246-3252 CC denotes either
T13732 3253-3255 IN denotes in
T13733 3256-3258 NNP denotes E.
T13734 3259-3263 NNP denotes coli
T13735 3264-3266 CC denotes or
T13736 3267-3269 IN denotes in
T13737 3270-3279 JJ denotes mammalian
T13738 3280-3285 NNS denotes cells
T13739 3286-3287 -LRB- denotes [
T13740 3287-3289 CD denotes 36
T13741 3289-3290 -RRB- denotes ]
T13742 3290-3291 . denotes .
T13743 3291-3543 sentence denotes The different timing of lethality in the Capn4 knockouts might also be a consequence of the different genetic backgrounds of the two transgenic lines, which has been observed to influence the phenotype of transgenic mice on a number of occasions [37].
T13744 3292-3295 DT denotes The
T13746 3296-3305 JJ denotes different
T13745 3306-3312 NN denotes timing
T13748 3313-3315 IN denotes of
T13749 3316-3325 NN denotes lethality
T13750 3326-3328 IN denotes in
T13751 3329-3332 DT denotes the
T13753 3333-3338 NN denotes Capn4
T13752 3339-3348 NNS denotes knockouts
T13754 3349-3354 MD denotes might
T13755 3355-3359 RB denotes also
T13747 3360-3362 VB denotes be
T13756 3363-3364 DT denotes a
T13757 3365-3376 NN denotes consequence
T13758 3377-3379 IN denotes of
T13759 3380-3383 DT denotes the
T13761 3384-3393 JJ denotes different
T13762 3394-3401 JJ denotes genetic
T13760 3402-3413 NNS denotes backgrounds
T13763 3414-3416 IN denotes of
T13764 3417-3420 DT denotes the
T13766 3421-3424 CD denotes two
T13767 3425-3435 JJ denotes transgenic
T13765 3436-3441 NNS denotes lines
T13768 3441-3443 , denotes ,
T13769 3443-3448 WDT denotes which
T13771 3449-3452 VBZ denotes has
T13772 3453-3457 VBN denotes been
T13770 3458-3466 VBN denotes observed
T13773 3467-3469 TO denotes to
T13774 3470-3479 VB denotes influence
T13775 3480-3483 DT denotes the
T13776 3484-3493 NN denotes phenotype
T13777 3494-3496 IN denotes of
T13778 3497-3507 JJ denotes transgenic
T13779 3508-3512 NNS denotes mice
T13780 3513-3515 IN denotes on
T13781 3516-3517 DT denotes a
T13782 3518-3524 NN denotes number
T13783 3525-3527 IN denotes of
T13784 3528-3537 NNS denotes occasions
T13785 3538-3539 -LRB- denotes [
T13786 3539-3541 CD denotes 37
T13787 3541-3542 -RRB- denotes ]
T13788 3542-3543 . denotes .
R2639 T13129 T13130 nsubj We,report
R2641 T13131 T13130 advmod here,report
R2642 T13132 T13133 mark that,die
R2644 T13133 T13130 ccomp die,report
R2645 T13134 T13135 nmod Capn2,mice
R2646 T13135 T13133 nsubj mice,die
R2647 T13136 T13134 punct -,Capn2
R2648 T13137 T13134 punct /,Capn2
R2649 T13138 T13134 punct -,Capn2
R2651 T13139 T13135 punct ", ",mice
R2652 T13140 T13141 dep which,lack
R2653 T13141 T13135 relcl lack,mice
R2655 T13142 T13143 det the,subunit
R2656 T13143 T13141 dobj subunit,lack
R2657 T13144 T13145 nmod m,calpain
R2659 T13145 T13143 nmod calpain,subunit
R2660 T13146 T13145 punct -,calpain
R2661 T13147 T13143 amod catalytic,subunit
R2663 T13148 T13133 punct ", ",die
R2664 T13149 T13133 prep at,die
R2665 T13150 T13151 det the,stage
R2667 T13151 T13149 pobj stage,at
R2668 T13152 T13151 compound preimplantation,stage
R2669 T13153 T13151 prep of,stage
R2670 T13154 T13153 pobj development,of
R2671 T13155 T13130 punct .,report
R2673 T13157 T13158 det This,observation
R2676 T13158 T13159 nsubj observation,allows
R2678 T13160 T13161 nsubj us,conclude
R2679 T13161 T13159 ccomp conclude,allows
R2681 T13162 T13161 aux to,conclude
R2682 T13163 T13161 advmod now,conclude
R2683 T13164 T13165 mark that,required
R2685 T13165 T13161 ccomp required,conclude
R2686 T13166 T13167 compound m,calpain
R2688 T13167 T13165 nsubjpass calpain,required
R2689 T13168 T13167 punct -,calpain
R2690 T13169 T13165 auxpass is,required
R2692 T13170 T13165 advmod specifically,required
R2693 T13171 T13165 prep during,required
R2694 T13172 T13171 pobj embryogenesis,during
R2696 T13173 T13159 punct .,allows
R2698 T13175 T13176 mark Since,expected
R2699 T13176 T13185 advcl expected,is
R2700 T13177 T13178 amod homozygous,disruption
R2702 T13178 T13176 nsubjpass disruption,expected
R2704 T13179 T13178 prep of,disruption
R2705 T13180 T13181 det the,gene
R2706 T13181 T13179 pobj gene,of
R2708 T13182 T13181 compound Capn4,gene
R2709 T13183 T13176 auxpass was,expected
R2711 T13184 T13176 advmod also,expected
R2712 T13186 T13187 aux to,abolish
R2713 T13187 T13176 xcomp abolish,expected
R2715 T13188 T13189 compound m,calpain
R2716 T13189 T13191 compound calpain,activity
R2717 T13190 T13189 punct -,calpain
R2719 T13191 T13187 dobj activity,abolish
R2720 T13192 T13185 punct ", ",is
R2722 T13193 T13194 det this,result
R2723 T13194 T13185 nsubj result,is
R2725 T13195 T13185 prep in,is
R2727 T13196 T13195 pobj agreement,in
R2728 T13197 T13196 prep with,agreement
R2729 T13198 T13199 det the,phenotype
R2730 T13199 T13197 pobj phenotype,with
R2731 T13200 T13199 acl presented,phenotype
R2733 T13201 T13200 agent by,presented
R2734 T13202 T13201 pobj one,by
R2735 T13203 T13202 prep of,one
R2737 T13204 T13205 det the,lines
R2738 T13205 T13203 pobj lines,of
R2739 T13299 T13300 det the,cassette
R2740 T13206 T13205 nummod two,lines
R2741 T13207 T13208 npadvmod Capn4,targeted
R2742 T13208 T13205 amod targeted,lines
R2743 T13209 T13210 prep in,observed
R2744 T13300 T13298 pobj cassette,of
R2745 T13210 T13205 relcl observed,lines
R2746 T13211 T13209 pobj which,in
R2747 T13212 T13213 compound preimplantation,lethality
R2748 T13301 T13302 compound PGK,Neo
R2749 T13213 T13210 nsubjpass lethality,observed
R2750 T13214 T13213 prep of,lethality
R2751 T13215 T13216 amod null,embryos
R2752 T13216 T13214 pobj embryos,of
R2753 T13302 T13300 compound Neo,cassette
R2754 T13217 T13210 auxpass was,observed
R2755 T13218 T13210 advmod also,observed
R2756 T13219 T13220 punct [,32
R2757 T13303 T13302 punct -,Neo
R2758 T13304 T13297 prep into,insertion
R2759 T13220 T13210 parataxis 32,observed
R2760 T13305 T13306 det the,middle
R2761 T13221 T13220 punct ],32
R2762 T13222 T13185 punct .,is
R2763 T13306 T13304 pobj middle,into
R2764 T13224 T13225 det The,survival
R2765 T13307 T13306 prep of,middle
R2766 T13225 T13226 nsubj survival,is
R2767 T13308 T13307 pobj exon,of
R2768 T13227 T13225 prep of,survival
R2769 T13228 T13229 nmod Capn4,embryos
R2770 T13229 T13227 pobj embryos,of
R2771 T13230 T13228 punct -,Capn4
R2772 T13231 T13228 punct /,Capn4
R2773 T13232 T13228 punct -,Capn4
R2774 T13233 T13225 prep from,survival
R2775 T13309 T13308 nummod 9,exon
R2776 T13234 T13235 det the,line
R2777 T13235 T13233 pobj line,from
R2778 T13236 T13235 amod original,line
R2779 T13310 T13297 punct ", ",insertion
R2780 T13237 T13235 amod targeted,line
R2781 T13238 T13235 acl reported,line
R2782 T13311 T13312 dep which,caused
R2783 T13239 T13238 agent by,reported
R2784 T13240 T13239 pobj Arthur,by
R2785 T13241 T13240 cc and,Arthur
R2786 T13312 T13297 relcl caused,insertion
R2787 T13242 T13240 conj colleagues,Arthur
R2788 T13313 T13312 dobj truncation,caused
R2789 T13314 T13313 prep of,truncation
R2790 T13243 T13233 prep to,from
R2791 T13244 T13243 pobj mid-gestation,to
R2792 T13315 T13316 det the,protein
R2793 T13245 T13246 advmod more,difficult
R2794 T13246 T13226 acomp difficult,is
R2795 T13316 T13314 pobj protein,of
R2796 T13247 T13248 aux to,reconcile
R2797 T13248 T13246 advcl reconcile,difficult
R2798 T13249 T13250 punct [,31
R2799 T13317 T13318 punct [,31
R2800 T13250 T13226 parataxis 31,is
R2801 T13251 T13250 punct ],31
R2802 T13318 T13312 parataxis 31,caused
R2803 T13252 T13226 punct .,is
R2804 T13254 T13255 prep In,seems
R2805 T13319 T13318 punct ],31
R2806 T13256 T13254 pobj retrospect,In
R2807 T13320 T13280 punct .,involved
R2808 T13257 T13255 punct ", ",seems
R2809 T13258 T13255 nsubj it,seems
R2810 T13259 T13255 oprd likely,seems
R2811 T13322 T13323 det This,strategy
R2812 T13260 T13261 mark that,represents
R2813 T13261 T13255 ccomp represents,seems
R2814 T13262 T13263 det the,line
R2815 T13263 T13261 nsubj line,represents
R2816 T13323 T13324 nsubjpass strategy,based
R2817 T13264 T13263 amod latter,line
R2818 T13265 T13266 det a,state
R2819 T13325 T13324 auxpass was,based
R2820 T13266 T13261 dobj state,represents
R2821 T13267 T13266 amod hypomorphic,state
R2822 T13268 T13266 punct ", ",state
R2823 T13269 T13270 advmod rather,than
R2824 T13270 T13266 cc than,state
R2825 T13271 T13272 det a,null
R2826 T13272 T13266 conj null,state
R2827 T13273 T13272 amod true,null
R2828 T13274 T13255 punct .,seems
R2829 T13326 T13324 prep upon,based
R2830 T13276 T13277 det The,strategy
R2831 T13277 T13280 nsubj strategy,involved
R2832 T13327 T13328 amod previous,studies
R2833 T13278 T13277 compound Capn4,strategy
R2834 T13279 T13277 compound targeting,strategy
R2835 T13328 T13326 pobj studies,upon
R2836 T13281 T13277 acl employed,strategy
R2837 T13282 T13281 agent by,employed
R2838 T13283 T13282 pobj Arthur,by
R2839 T13284 T13283 cc and,Arthur
R2840 T13285 T13283 conj colleagues,Arthur
R2841 T13329 T13330 compound structure,function
R2842 T13286 T13280 xcomp disrupting,involved
R2843 T13287 T13288 det the,terminus
R2844 T13288 T13286 dobj terminus,disrupting
R2845 T13330 T13328 compound function,studies
R2846 T13289 T13288 compound C,terminus
R2847 T13290 T13288 punct -,terminus
R2848 T13291 T13288 prep of,terminus
R2849 T13292 T13293 det the,subunit
R2850 T13331 T13330 punct /,function
R2851 T13293 T13291 pobj subunit,of
R2852 T13294 T13293 nmod calpain,subunit
R2853 T13332 T13328 acl showing,studies
R2854 T13295 T13293 amod small,subunit
R2855 T13296 T13286 agent by,disrupting
R2856 T13297 T13296 pobj insertion,by
R2857 T13333 T13334 mark that,abolished
R2858 T13298 T13297 prep of,insertion
R2859 T13334 T13332 ccomp abolished,showing
R2860 T13335 T13334 nsubj excision,abolished
R2861 T13336 T13335 prep of,excision
R2862 T13405 T13406 det the,time
R2863 T13406 T13404 pobj time,in
R2864 T13337 T13338 det the,acid
R2865 T13407 T13406 prep of,time
R2866 T13408 T13407 pobj lethality,of
R2867 T13409 T13406 prep of,time
R2868 T13338 T13336 pobj acid,of
R2869 T13410 T13411 det these,lines
R2870 T13339 T13340 npadvmod C,terminal
R2871 T13340 T13338 amod terminal,acid
R2872 T13411 T13409 pobj lines,of
R2873 T13341 T13340 punct -,terminal
R2874 T13412 T13411 nummod two,lines
R2875 T13413 T13411 amod targeted,lines
R2876 T13414 T13411 compound Capn4,lines
R2877 T13415 T13401 aux can,explained
R2878 T13416 T13401 auxpass be,explained
R2879 T13417 T13401 agent by,explained
R2880 T13418 T13419 amod different,extents
R2881 T13342 T13338 nummod 25,acid
R2882 T13419 T13417 pobj extents,by
R2883 T13420 T13419 prep of,extents
R2884 T13421 T13420 pobj disruption,of
R2885 T13343 T13338 compound amino,acid
R2886 T13422 T13397 punct .,seems
R2887 T13344 T13338 nmod residues,acid
R2888 T13424 T13425 det The,allele
R2889 T13425 T13427 nsubj allele,represents
R2890 T13345 T13338 prep of,acid
R2891 T13426 T13425 compound Zimmerman,allele
R2892 T13346 T13347 det the,subunit
R2893 T13428 T13427 advmod probably,represents
R2894 T13429 T13430 det a,genotype
R2895 T13430 T13427 dobj genotype,represents
R2896 T13347 T13345 pobj subunit,of
R2897 T13431 T13430 amod true,genotype
R2898 T13432 T13430 amod null,genotype
R2899 T13433 T13434 mark while,is
R2900 T13348 T13347 amod small,subunit
R2901 T13434 T13427 advcl is,represents
R2902 T13435 T13436 det the,allele
R2903 T13436 T13434 nsubj allele,is
R2904 T13349 T13350 compound calpain,activity
R2905 T13437 T13436 compound Arthur,allele
R2906 T13438 T13434 advmod likely,is
R2907 T13439 T13440 det a,mutation
R2908 T13350 T13334 dobj activity,abolished
R2909 T13440 T13434 attr mutation,is
R2910 T13441 T13440 amod hypomorphic,mutation
R2911 T13442 T13427 punct .,represents
R2912 T13351 T13352 advmod when,co-expressed
R2913 T13444 T13445 amod Alternate,strategies
R2914 T13352 T13334 advcl co-expressed,abolished
R2915 T13445 T13447 nsubjpass strategies,shown
R2916 T13446 T13445 compound targeting,strategies
R2917 T13353 T13352 prep with,co-expressed
R2918 T13448 T13447 aux have,shown
R2919 T13449 T13447 auxpass been,shown
R2920 T13450 T13451 aux to,yield
R2921 T13354 T13355 det the,subunit
R2922 T13451 T13447 advcl yield,shown
R2923 T13452 T13453 amod different,phenotypes
R2924 T13453 T13451 dobj phenotypes,yield
R2925 T13355 T13353 pobj subunit,with
R2926 T13454 T13451 prep in,yield
R2927 T13455 T13456 compound gene,disruption
R2928 T13456 T13457 compound disruption,studies
R2929 T13457 T13454 pobj studies,in
R2930 T13458 T13447 punct .,shown
R2931 T13460 T13461 prep For,used
R2932 T13356 T13357 nmod m,calpain
R2933 T13462 T13460 pobj example,For
R2934 T13463 T13461 punct ", ",used
R2935 T13464 T13465 nummod three,strategies
R2936 T13465 T13461 nsubjpass strategies,used
R2937 T13466 T13465 amod different,strategies
R2938 T13357 T13355 nmod calpain,subunit
R2939 T13467 T13465 compound targeting,strategies
R2940 T13468 T13461 auxpass were,used
R2941 T13469 T13461 advmod independently,used
R2942 T13358 T13357 punct -,calpain
R2943 T13470 T13471 aux to,disrupt
R2944 T13471 T13461 advcl disrupt,used
R2945 T13359 T13355 amod large,subunit
R2946 T13472 T13473 det the,gene
R2947 T13473 T13471 dobj gene,disrupt
R2948 T13474 T13475 amod mixed,leukemia
R2949 T13475 T13473 nmod leukemia,gene
R2950 T13360 T13352 prep in,co-expressed
R2951 T13476 T13475 nmod lineage,leukemia
R2952 T13477 T13475 punct (,leukemia
R2953 T13361 T13362 compound E.,coli
R2954 T13478 T13475 appos Mll,leukemia
R2955 T13479 T13473 punct ),gene
R2956 T13480 T13461 punct .,used
R2957 T13362 T13360 pobj coli,in
R2958 T13482 T13483 prep In,perished
R2959 T13484 T13485 det all,studies
R2960 T13363 T13364 punct [,34
R2961 T13485 T13482 pobj studies,In
R2962 T13486 T13485 nummod three,studies
R2963 T13487 T13483 punct ", ",perished
R2964 T13364 T13334 parataxis 34,abolished
R2965 T13488 T13489 amod homozygous,null
R2966 T13489 T13490 amod null,embryos
R2967 T13490 T13483 nsubj embryos,perished
R2968 T13365 T13364 punct ],34
R2969 T13491 T13483 prep during,perished
R2970 T13492 T13491 pobj embryogenesis,during
R2971 T13366 T13324 punct .,based
R2972 T13493 T13491 punct ", ",during
R2973 T13494 T13491 cc but,during
R2974 T13495 T13491 conj at,during
R2975 T13496 T13497 amod different,stages
R2976 T13368 T13369 prep In,involved
R2977 T13497 T13495 pobj stages,at
R2978 T13498 T13499 punct (,E14.5
R2979 T13499 T13497 parataxis E14.5,stages
R2980 T13500 T13499 nmod E0.5,E14.5
R2981 T13501 T13499 punct ", ",E14.5
R2982 T13370 T13368 pobj contrast,In
R2983 T13502 T13499 nmod E10.5,E14.5
R2984 T13503 T13499 punct ", ",E14.5
R2985 T13504 T13499 punct ),E14.5
R2986 T13505 T13506 punct [,35
R2987 T13506 T13483 parataxis 35,perished
R2988 T13371 T13369 punct ", ",involved
R2989 T13507 T13506 punct ],35
R2990 T13508 T13483 punct .,perished
R2991 T13372 T13373 det the,strategy
R2992 T13510 T13511 det The,variation
R2993 T13373 T13369 nsubj strategy,involved
R2994 T13374 T13373 nmod Capn4,strategy
R2995 T13375 T13373 amod targeting,strategy
R2996 T13511 T13512 nsubjpass variation,attributed
R2997 T13376 T13373 acl employed,strategy
R2998 T13513 T13511 prep in,variation
R2999 T13377 T13376 agent by,employed
R3000 T13514 T13513 pobj phenotype,in
R3001 T13515 T13512 auxpass was,attributed
R3002 T13516 T13512 prep to,attributed
R3003 T13517 T13518 det the,differences
R3004 T13378 T13377 pobj Zimmerman,by
R3005 T13518 T13516 pobj differences,to
R3006 T13519 T13518 prep in,differences
R3007 T13520 T13519 pobj degree,in
R3008 T13379 T13378 cc and,Zimmerman
R3009 T13521 T13520 prep of,degree
R3010 T13522 T13521 pobj function,of
R3011 T13523 T13520 prep of,degree
R3012 T13380 T13378 conj colleagues,Zimmerman
R3013 T13524 T13525 det the,proteins
R3014 T13525 T13523 pobj proteins,of
R3015 T13526 T13525 amod truncated,proteins
R3016 T13381 T13382 det a,deletion
R3017 T13527 T13525 acl produced,proteins
R3018 T13528 T13527 prep from,produced
R3019 T13529 T13530 det the,alleles
R3020 T13382 T13369 dobj deletion,involved
R3021 T13530 T13528 pobj alleles,from
R3022 T13531 T13530 compound mutant,alleles
R3023 T13532 T13512 punct .,attributed
R3024 T13383 T13384 advmod much,more
R3025 T13534 T13535 det A,effect
R3026 T13535 T13537 nsubj effect,be
R3027 T13384 T13385 advmod more,extensive
R3028 T13536 T13535 amod similar,effect
R3029 T13538 T13537 aux might,be
R3030 T13385 T13382 amod extensive,deletion
R3031 T13539 T13537 prep at,be
R3032 T13540 T13539 pobj work,at
R3033 T13541 T13537 prep in,be
R3034 T13542 T13543 det the,lines
R3035 T13543 T13541 pobj lines,in
R3036 T13544 T13543 nummod two,lines
R3037 T13386 T13382 prep of,deletion
R3038 T13545 T13546 npadvmod Capn4,transgenic
R3039 T13546 T13543 amod transgenic,lines
R3040 T13547 T13537 punct .,be
R3041 T13387 T13388 nmod exons,4
R3042 T13549 T13550 nsubjpass Efforts,made
R3043 T13388 T13386 pobj 4,of
R3044 T13551 T13550 auxpass were,made
R3045 T13552 T13553 aux to,detect
R3046 T13553 T13550 advcl detect,made
R3047 T13389 T13388 prep through,4
R3048 T13554 T13553 dobj transcripts,detect
R3049 T13555 T13554 cc or,transcripts
R3050 T13390 T13389 pobj 8,through
R3051 T13556 T13557 compound calpain,activities
R3052 T13557 T13554 conj activities,transcripts
R3053 T13391 T13392 punct [,32
R3054 T13558 T13554 acl derived,transcripts
R3055 T13559 T13558 prep from,derived
R3056 T13560 T13561 det the,allele
R3057 T13561 T13559 pobj allele,from
R3058 T13392 T13369 parataxis 32,involved
R3059 T13562 T13563 compound Arthur,Capn4
R3060 T13563 T13561 compound Capn4,allele
R3061 T13564 T13550 punct .,made
R3062 T13393 T13392 punct ],32
R3063 T13566 T13567 det This,allele
R3064 T13567 T13568 nsubj allele,gave
R3065 T13394 T13369 punct .,involved
R3066 T13569 T13568 dobj rise,gave
R3067 T13396 T13397 nsubj It,seems
R3068 T13570 T13568 prep to,gave
R3069 T13571 T13572 amod multiple,species
R3070 T13572 T13570 pobj species,to
R3071 T13573 T13572 compound mRNA,species
R3072 T13398 T13397 advmod now,seems
R3073 T13574 T13572 punct ", ",species
R3074 T13575 T13572 amod detectable,species
R3075 T13576 T13575 prep by,detectable
R3076 T13577 T13578 compound RT,PCR
R3077 T13399 T13397 oprd probable,seems
R3078 T13578 T13576 pobj PCR,by
R3079 T13579 T13578 punct -,PCR
R3080 T13580 T13568 punct ", ",gave
R3081 T13400 T13401 mark that,explained
R3082 T13581 T13568 advcl reading,gave
R3083 T13582 T13581 advmod through,reading
R3084 T13583 T13581 prep from,reading
R3085 T13584 T13585 det the,half
R3086 T13585 T13583 pobj half,from
R3087 T13401 T13397 ccomp explained,seems
R3088 T13586 T13585 amod first,half
R3089 T13587 T13585 prep of,half
R3090 T13588 T13587 pobj exon,of
R3091 T13402 T13403 det the,difference
R3092 T13403 T13401 nsubjpass difference,explained
R3093 T13589 T13588 nummod 9,exon
R3094 T13590 T13583 prep to,from
R3095 T13404 T13403 prep in,difference
R3096 T13591 T13592 advmod at,two
R3097 T13592 T13594 nummod two,sites
R3098 T13593 T13592 advmod least,two
R3099 T13617 T13615 amod small,subunits
R3100 T13594 T13590 pobj sites,to
R3101 T13595 T13594 amod different,sites
R3102 T13596 T13594 amod cryptic,sites
R3103 T13597 T13594 compound splice,sites
R3104 T13618 T13610 prep with,give
R3105 T13598 T13594 prep in,sites
R3106 T13599 T13600 det the,sequence
R3107 T13619 T13620 quantmod 10,30
R3108 T13600 T13598 pobj sequence,in
R3109 T13601 T13600 compound PGK,sequence
R3110 T13602 T13600 compound promoter,sequence
R3111 T13620 T13622 nummod 30,acids
R3112 T13603 T13604 punct [,31
R3113 T13604 T13581 parataxis 31,reading
R3114 T13605 T13604 punct ],31
R3115 T13621 T13620 punct –,30
R3116 T13606 T13568 punct .,gave
R3117 T13608 T13609 det These,transcripts
R3118 T13622 T13618 pobj acids,with
R3119 T13609 T13610 nsubj transcripts,give
R3120 T13611 T13610 aux could,give
R3121 T13623 T13622 amod inappropriate,acids
R3122 T13612 T13610 dobj rise,give
R3123 T13613 T13610 prep to,give
R3124 T13614 T13615 amod defective,subunits
R3125 T13624 T13625 npadvmod C,terminal
R3126 T13615 T13613 pobj subunits,to
R3127 T13625 T13622 amod terminal,acids
R3128 T13626 T13625 punct -,terminal
R3129 T13616 T13615 nmod calpain,subunits
R3130 T13627 T13622 punct ", ",acids
R3131 T13628 T13629 dep which,be
R3132 T13629 T13622 relcl be,acids
R3133 T13723 T13721 pobj hands,in
R3134 T13630 T13629 aux might,be
R3135 T13724 T13725 advmod when,expressed
R3136 T13725 T13719 advcl expressed,observed
R3137 T13726 T13727 nmod calpain,subunits
R3138 T13727 T13725 nsubjpass subunits,expressed
R3139 T13728 T13727 amod large,subunits
R3140 T13729 T13725 auxpass were,expressed
R3141 T13631 T13629 acomp sufficient,be
R3142 T13730 T13725 advmod alone,expressed
R3143 T13731 T13732 preconj either,in
R3144 T13732 T13725 prep in,expressed
R3145 T13632 T13633 aux to,support
R3146 T13733 T13734 compound E.,coli
R3147 T13734 T13732 pobj coli,in
R3148 T13735 T13732 cc or,in
R3149 T13633 T13631 xcomp support,sufficient
R3150 T13736 T13732 conj in,in
R3151 T13737 T13738 amod mammalian,cells
R3152 T13634 T13635 det a,level
R3153 T13738 T13736 pobj cells,in
R3154 T13739 T13740 punct [,36
R3155 T13740 T13725 parataxis 36,expressed
R3156 T13635 T13633 dobj level,support
R3157 T13741 T13740 punct ],36
R3158 T13742 T13655 punct .,suggested
R3159 T13636 T13635 amod low,level
R3160 T13744 T13745 det The,timing
R3161 T13745 T13747 nsubj timing,be
R3162 T13637 T13635 prep of,level
R3163 T13746 T13745 amod different,timing
R3164 T13638 T13639 compound calpain,activity
R3165 T13748 T13745 prep of,timing
R3166 T13749 T13748 pobj lethality,of
R3167 T13750 T13745 prep in,timing
R3168 T13639 T13637 pobj activity,of
R3169 T13751 T13752 det the,knockouts
R3170 T13752 T13750 pobj knockouts,in
R3171 T13753 T13752 compound Capn4,knockouts
R3172 T13640 T13610 punct .,give
R3173 T13754 T13747 aux might,be
R3174 T13755 T13747 advmod also,be
R3175 T13756 T13757 det a,consequence
R3176 T13642 T13643 advmod However,give
R3177 T13643 T13655 ccomp give,suggested
R3178 T13757 T13747 attr consequence,be
R3179 T13758 T13757 prep of,consequence
R3180 T13644 T13643 punct ", ",give
R3181 T13759 T13760 det the,backgrounds
R3182 T13760 T13758 pobj backgrounds,of
R3183 T13761 T13760 amod different,backgrounds
R3184 T13645 T13643 nsubj expression,give
R3185 T13762 T13760 amod genetic,backgrounds
R3186 T13763 T13760 prep of,backgrounds
R3187 T13764 T13765 det the,lines
R3188 T13765 T13763 pobj lines,of
R3189 T13766 T13765 nummod two,lines
R3190 T13646 T13645 prep of,expression
R3191 T13767 T13765 amod transgenic,lines
R3192 T13768 T13757 punct ", ",consequence
R3193 T13769 T13770 dep which,observed
R3194 T13647 T13646 pobj calpains,of
R3195 T13770 T13757 relcl observed,consequence
R3196 T13771 T13770 aux has,observed
R3197 T13772 T13770 auxpass been,observed
R3198 T13648 T13647 prep with,calpains
R3199 T13773 T13774 aux to,influence
R3200 T13774 T13770 advcl influence,observed
R3201 T13649 T13650 det these,subunits
R3202 T13775 T13776 det the,phenotype
R3203 T13776 T13774 dobj phenotype,influence
R3204 T13777 T13776 prep of,phenotype
R3205 T13650 T13648 pobj subunits,with
R3206 T13778 T13779 amod transgenic,mice
R3207 T13779 T13777 pobj mice,of
R3208 T13651 T13650 amod modified,subunits
R3209 T13780 T13774 prep on,influence
R3210 T13781 T13782 det a,number
R3211 T13782 T13780 pobj number,on
R3212 T13652 T13650 amod small,subunits
R3213 T13783 T13782 prep of,number
R3214 T13784 T13783 pobj occasions,of
R3215 T13785 T13786 punct [,37
R3216 T13653 T13643 aux did,give
R3217 T13786 T13774 parataxis 37,influence
R3218 T13787 T13786 punct ],37
R3219 T13654 T13643 neg not,give
R3220 T13788 T13747 punct .,be
R3222 T13656 T13643 dobj rise,give
R3226 T13657 T13643 prep to,give
R3230 T13658 T13659 det any,activity
R3234 T13659 T13657 pobj activity,to
R3238 T13660 T13659 amod detectable,activity
R3244 T13661 T13659 compound calpain,activity
R3249 T13662 T13663 advmod when,expressed
R3252 T13663 T13643 advcl expressed,give
R3256 T13664 T13663 prep in,expressed
R3259 T13665 T13666 compound E.,coli
R3262 T13666 T13664 pobj coli,in
R3265 T13667 T13643 punct ", ",give
R3269 T13668 T13669 mark although,has
R3270 T13669 T13643 advcl has,give
R3271 T13670 T13671 poss their,functionality
R3274 T13671 T13669 nsubj functionality,has
R3278 T13672 T13671 prep in,functionality
R3281 T13673 T13674 amod mammalian,cells
R3284 T13674 T13672 pobj cells,in
R3286 T13675 T13669 advmod yet,has
R3290 T13676 T13677 aux to,determined
R3291 T13677 T13669 xcomp determined,has
R3294 T13678 T13677 auxpass be,determined
R3298 T13679 T13680 punct (,J.S.
R3301 T13680 T13669 meta J.S.,has
R3304 T13681 T13680 nmod Elce,J.S.
R3308 T13682 T13680 punct ", ",J.S.
R3311 T13683 T13680 amod unpublished,J.S.
R3315 T13684 T13680 nmod work,J.S.
R3319 T13685 T13680 punct ),J.S.
R3322 T13686 T13655 nsubjpass It,suggested
R3324 T13687 T13655 aux has,suggested
R3326 T13688 T13655 advmod also,suggested
R3330 T13689 T13655 auxpass been,suggested
R3334 T13690 T13691 mark that,provide
R3338 T13691 T13655 ccomp provide,suggested
R3342 T13692 T13693 nmod calpain,subunits
R3348 T13693 T13691 nsubj subunits,provide
R3352 T13694 T13693 amod large,subunits
R3355 T13695 T13693 advmod alone,subunits
R3358 T13696 T13691 aux might,provide
R3362 T13697 T13698 det some,activity
R3365 T13698 T13691 dobj activity,provide
R3367 T13699 T13691 prep in,provide
R3371 T13700 T13701 amod eukaryotic,cells
R3375 T13701 T13699 pobj cells,in
R3379 T13702 T13691 punct ", ",provide
R3382 T13703 T13704 mark although,appears
R3386 T13704 T13691 advcl appears,provide
R3390 T13705 T13706 det the,knockout
R3394 T13706 T13704 nsubj knockout,appears
R3400 T13707 T13706 nmod Zimmerman,knockout
R3403 T13708 T13709 advmod et,al.
R3405 T13709 T13707 advmod al.,Zimmerman
R3408 T13710 T13711 aux to,exclude
R3410 T13711 T13704 xcomp exclude,appears
R3412 T13712 T13713 det that,possibility
R3413 T13713 T13711 dobj possibility,exclude
R3415 T13714 T13691 punct ", ",provide
R3418 T13715 T13691 cc and,provide
R3422 T13716 T13717 det no,activity
R3425 T13717 T13719 nsubjpass activity,observed
R3429 T13718 T13717 compound calpain,activity
R3433 T13719 T13691 conj observed,provide
R3436 T13720 T13719 auxpass was,observed
R3440 T13721 T13719 prep in,observed
R3443 T13722 T13723 poss our,hands