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PMC:1382200 / 13047-14207 JSONTXT 4 Projects

Annnotations TAB TSV DIC JSON TextAE

Id Subject Object Predicate Lexical cue
T22640 10-20 NN denotes Genotyping
T22641 21-23 IN denotes of
T22642 24-40 JJ denotes pre-implantation
T22643 41-48 NNS denotes embryos
T22644 48-49 . denotes .
T22645 49-148 sentence denotes A nested PCR strategy was used to genotype embryos prior to implantation as described in Figure 4.
T22646 50-51 DT denotes A
T22648 52-58 VBN denotes nested
T22649 59-62 NN denotes PCR
T22647 63-71 NN denotes strategy
T22651 72-75 VBD denotes was
T22650 76-80 VBN denotes used
T22652 81-83 TO denotes to
T22653 84-92 VB denotes genotype
T22654 93-100 NNS denotes embryos
T22655 101-106 JJ denotes prior
T22656 107-109 IN denotes to
T22657 110-122 NN denotes implantation
T22658 123-125 IN denotes as
T22659 126-135 VBN denotes described
T22660 136-138 IN denotes in
T22661 139-145 NN denotes Figure
T22662 146-147 CD denotes 4
T22663 147-148 . denotes .
T22664 148-251 sentence denotes Capn2+/- mice were mated and the date of fertilization established by the appearance of vaginal plugs.
T22665 149-154 NN denotes Capn2
T22667 154-155 SYM denotes +
T22668 155-156 HYPH denotes /
T22669 156-157 SYM denotes -
T22666 158-162 NNS denotes mice
T22671 163-167 VBD denotes were
T22670 168-173 VBN denotes mated
T22672 174-177 CC denotes and
T22673 178-181 DT denotes the
T22674 182-186 NN denotes date
T22676 187-189 IN denotes of
T22677 190-203 NN denotes fertilization
T22675 204-215 VBN denotes established
T22678 216-218 IN denotes by
T22679 219-222 DT denotes the
T22680 223-233 NN denotes appearance
T22681 234-236 IN denotes of
T22682 237-244 JJ denotes vaginal
T22683 245-250 NNS denotes plugs
T22684 250-251 . denotes .
T22685 251-362 sentence denotes Blastocyst (E3.5) or 8-cell embryos (E2.5) were flushed from the oviducts and then digested with proteinase K.
T22686 252-262 NN denotes Blastocyst
T22688 263-264 -LRB- denotes (
T22689 264-268 NN denotes E3.5
T22690 268-269 -RRB- denotes )
T22691 270-272 CC denotes or
T22692 273-274 CD denotes 8
T22694 274-275 HYPH denotes -
T22693 275-279 NN denotes cell
T22687 280-287 NNS denotes embryos
T22696 288-289 -LRB- denotes (
T22697 289-293 NN denotes E2.5
T22698 293-294 -RRB- denotes )
T22699 295-299 VBD denotes were
T22695 300-307 VBN denotes flushed
T22700 308-312 IN denotes from
T22701 313-316 DT denotes the
T22702 317-325 NNS denotes oviducts
T22703 326-329 CC denotes and
T22704 330-334 RB denotes then
T22705 335-343 VBN denotes digested
T22706 344-348 IN denotes with
T22707 349-359 NN denotes proteinase
T22708 360-361 NN denotes K
T22709 361-362 . denotes .
T22710 362-599 sentence denotes In separate reactions segments found exclusively in either the wild-type or mutant alleles were co-amplified with an internal control sequence, located in the short (upstream) arm of the targeting vector, which is found in both alleles.
T22711 363-365 IN denotes In
T22713 366-374 JJ denotes separate
T22715 375-384 NNS denotes reactions
T22714 385-393 NNS denotes segments
T22716 394-399 VBN denotes found
T22717 400-411 RB denotes exclusively
T22718 412-414 IN denotes in
T22719 415-421 CC denotes either
T22721 422-425 DT denotes the
T22722 426-430 JJ denotes wild
T22723 430-431 HYPH denotes -
T22720 431-435 NN denotes type
T22724 436-438 CC denotes or
T22725 439-445 NN denotes mutant
T22726 446-453 NNS denotes alleles
T22727 454-458 VBD denotes were
T22712 459-471 VBN denotes co-amplified
T22728 472-476 IN denotes with
T22729 477-479 DT denotes an
T22731 480-488 JJ denotes internal
T22732 489-496 NN denotes control
T22730 497-505 NN denotes sequence
T22733 505-507 , denotes ,
T22734 507-514 VBN denotes located
T22735 515-517 IN denotes in
T22736 518-521 DT denotes the
T22738 522-527 JJ denotes short
T22739 528-529 -LRB- denotes (
T22740 529-537 JJ denotes upstream
T22741 537-538 -RRB- denotes )
T22737 539-542 NN denotes arm
T22742 543-545 IN denotes of
T22743 546-549 DT denotes the
T22745 550-559 NN denotes targeting
T22744 560-566 NN denotes vector
T22746 566-568 , denotes ,
T22747 568-573 WDT denotes which
T22749 574-576 VBZ denotes is
T22748 577-582 VBN denotes found
T22750 583-585 IN denotes in
T22751 586-590 DT denotes both
T22752 591-598 NNS denotes alleles
T22753 598-599 . denotes .
T22754 599-723 sentence denotes The final products were 429 bp for the wild-type allele, 389 bp for the mutant allele, and 213 bp for the internal control.
T22755 600-603 DT denotes The
T22757 604-609 JJ denotes final
T22756 610-618 NNS denotes products
T22758 619-623 VBD denotes were
T22759 624-627 CD denotes 429
T22760 628-630 NN denotes bp
T22761 631-634 IN denotes for
T22762 635-638 DT denotes the
T22764 639-643 JJ denotes wild
T22766 643-644 HYPH denotes -
T22765 644-648 NN denotes type
T22763 649-655 NN denotes allele
T22767 655-657 , denotes ,
T22768 657-660 CD denotes 389
T22769 661-663 NN denotes bp
T22770 664-667 IN denotes for
T22771 668-671 DT denotes the
T22773 672-678 NN denotes mutant
T22772 679-685 NN denotes allele
T22774 685-687 , denotes ,
T22775 687-690 CC denotes and
T22776 691-694 CD denotes 213
T22777 695-697 NN denotes bp
T22778 698-701 IN denotes for
T22779 702-705 DT denotes the
T22781 706-714 JJ denotes internal
T22780 715-722 NN denotes control
T22782 722-723 . denotes .
T22783 723-799 sentence denotes (A) A representative example of the genotyping of blastocyst stage embryos.
T22784 724-725 -LRB- denotes (
T22785 725-726 LS denotes A
T22787 726-727 -RRB- denotes )
T22788 728-729 DT denotes A
T22789 730-744 JJ denotes representative
T22786 745-752 NN denotes example
T22790 753-755 IN denotes of
T22791 756-759 DT denotes the
T22792 760-770 NN denotes genotyping
T22793 771-773 IN denotes of
T22794 774-784 NN denotes blastocyst
T22796 785-790 NN denotes stage
T22795 791-798 NNS denotes embryos
T22797 798-799 . denotes .
T22798 799-933 sentence denotes Embryos #1, 2, 4, 5, and 6 were Capn2+/- whereas embryos #3 and #7 were Capn2+/+, denoted by the absence of the 389 bp mutant signal.
T22799 800-807 NNS denotes Embryos
T22801 808-809 SYM denotes #
T22800 809-810 CD denotes 1
T22803 810-812 , denotes ,
T22804 812-813 CD denotes 2
T22805 813-815 , denotes ,
T22806 815-816 CD denotes 4
T22807 816-818 , denotes ,
T22808 818-819 CD denotes 5
T22809 819-821 , denotes ,
T22810 821-824 CC denotes and
T22811 825-826 CD denotes 6
T22802 827-831 VBD denotes were
T22812 832-837 NN denotes Capn2
T22813 837-838 SYM denotes +
T22814 838-839 HYPH denotes /
T22815 839-840 SYM denotes -
T22816 841-848 IN denotes whereas
T22818 849-856 NNS denotes embryos
T22820 857-858 SYM denotes #
T22819 858-859 CD denotes 3
T22821 860-863 CC denotes and
T22822 864-865 SYM denotes #
T22823 865-866 CD denotes 7
T22817 867-871 VBD denotes were
T22824 872-877 NN denotes Capn2
T22825 877-878 SYM denotes +
T22826 878-879 HYPH denotes /
T22827 879-880 SYM denotes +
T22828 880-882 , denotes ,
T22829 882-889 VBN denotes denoted
T22830 890-892 IN denotes by
T22831 893-896 DT denotes the
T22832 897-904 NN denotes absence
T22833 905-907 IN denotes of
T22834 908-911 DT denotes the
T22836 912-915 CD denotes 389
T22837 916-918 NN denotes bp
T22838 919-925 NN denotes mutant
T22835 926-932 NN denotes signal
T22839 932-933 . denotes .
T22840 933-994 sentence denotes (B) An example of the genotyping of 8-cell embryos is shown.
T22841 934-935 -LRB- denotes (
T22842 935-936 LS denotes B
T22844 936-937 -RRB- denotes )
T22845 938-940 DT denotes An
T22846 941-948 NN denotes example
T22847 949-951 IN denotes of
T22848 952-955 DT denotes the
T22849 956-966 NN denotes genotyping
T22850 967-969 IN denotes of
T22851 970-971 CD denotes 8
T22853 971-972 HYPH denotes -
T22852 972-976 NN denotes cell
T22854 977-984 NNS denotes embryos
T22855 985-987 VBZ denotes is
T22843 988-993 VBN denotes shown
T22856 993-994 . denotes .
T22857 994-1119 sentence denotes Embryos #1, 2, 3, 5, and 6 were Capn2+/- while embryo #4 was Capn2-/-, marked by the absence of the 429 bp wild-type signal.
T22858 995-1002 NNS denotes Embryos
T22860 1003-1004 SYM denotes #
T22859 1004-1005 CD denotes 1
T22862 1005-1007 , denotes ,
T22863 1007-1008 CD denotes 2
T22864 1008-1010 , denotes ,
T22865 1010-1011 CD denotes 3
T22866 1011-1013 , denotes ,
T22867 1013-1014 CD denotes 5
T22868 1014-1016 , denotes ,
T22869 1016-1019 CC denotes and
T22870 1020-1021 CD denotes 6
T22861 1022-1026 VBD denotes were
T22871 1027-1032 NN denotes Capn2
T22872 1032-1033 SYM denotes +
T22873 1033-1034 HYPH denotes /
T22874 1034-1035 SYM denotes -
T22875 1036-1041 IN denotes while
T22877 1042-1048 NN denotes embryo
T22878 1049-1050 SYM denotes #
T22879 1050-1051 CD denotes 4
T22876 1052-1055 VBD denotes was
T22880 1056-1061 NN denotes Capn2
T22881 1061-1062 SYM denotes -
T22882 1062-1063 HYPH denotes /
T22883 1063-1064 SYM denotes -
T22884 1064-1066 , denotes ,
T22885 1066-1072 VBN denotes marked
T22886 1073-1075 IN denotes by
T22887 1076-1079 DT denotes the
T22888 1080-1087 NN denotes absence
T22889 1088-1090 IN denotes of
T22890 1091-1094 DT denotes the
T22892 1095-1098 CD denotes 429
T22893 1099-1101 NN denotes bp
T22894 1102-1106 JJ denotes wild
T22896 1106-1107 HYPH denotes -
T22895 1107-1111 NN denotes type
T22891 1112-1118 NN denotes signal
T22897 1118-1119 . denotes .
T22898 1119-1160 sentence denotes (M) denotes the molecular weight marker.
T22899 1120-1121 -LRB- denotes (
T22900 1121-1122 NN denotes M
T22902 1122-1123 -RRB- denotes )
T22901 1124-1131 VBZ denotes denotes
T22903 1132-1135 DT denotes the
T22905 1136-1145 JJ denotes molecular
T22906 1146-1152 NN denotes weight
T22904 1153-1159 NN denotes marker
T22907 1159-1160 . denotes .
R6683 T22641 T22640 prep of,Genotyping
R6684 T22642 T22643 amod pre-implantation,embryos
R6685 T22643 T22641 pobj embryos,of
R6686 T22644 T22640 punct .,Genotyping
R6687 T22646 T22647 det A,strategy
R6688 T22647 T22650 nsubjpass strategy,used
R6689 T22648 T22647 amod nested,strategy
R6690 T22649 T22647 compound PCR,strategy
R6691 T22651 T22650 auxpass was,used
R6692 T22652 T22653 aux to,genotype
R6693 T22653 T22650 advcl genotype,used
R6694 T22654 T22653 dobj embryos,genotype
R6695 T22655 T22656 amod prior,to
R6696 T22656 T22653 prep to,genotype
R6697 T22657 T22656 pobj implantation,to
R6698 T22658 T22659 mark as,described
R6699 T22659 T22653 advcl described,genotype
R6700 T22660 T22659 prep in,described
R6701 T22661 T22660 pobj Figure,in
R6702 T22662 T22661 nummod 4,Figure
R6703 T22663 T22650 punct .,used
R6704 T22665 T22666 nmod Capn2,mice
R6705 T22666 T22670 nsubjpass mice,mated
R6706 T22667 T22665 punct +,Capn2
R6707 T22668 T22665 punct /,Capn2
R6708 T22669 T22665 punct -,Capn2
R6709 T22671 T22670 auxpass were,mated
R6710 T22672 T22670 cc and,mated
R6711 T22673 T22674 det the,date
R6712 T22674 T22675 nsubj date,established
R6713 T22675 T22670 conj established,mated
R6714 T22676 T22674 prep of,date
R6715 T22677 T22676 pobj fertilization,of
R6716 T22678 T22675 agent by,established
R6717 T22679 T22680 det the,appearance
R6718 T22680 T22678 pobj appearance,by
R6719 T22681 T22680 prep of,appearance
R6720 T22682 T22683 amod vaginal,plugs
R6721 T22683 T22681 pobj plugs,of
R6722 T22684 T22675 punct .,established
R6723 T22686 T22687 nmod Blastocyst,embryos
R6724 T22687 T22695 nsubjpass embryos,flushed
R6725 T22688 T22689 punct (,E3.5
R6726 T22689 T22686 parataxis E3.5,Blastocyst
R6727 T22690 T22689 punct ),E3.5
R6728 T22691 T22686 cc or,Blastocyst
R6729 T22692 T22693 nummod 8,cell
R6730 T22693 T22686 conj cell,Blastocyst
R6731 T22694 T22693 punct -,cell
R6732 T22696 T22697 punct (,E2.5
R6733 T22697 T22687 parataxis E2.5,embryos
R6734 T22698 T22697 punct ),E2.5
R6735 T22699 T22695 auxpass were,flushed
R6736 T22700 T22695 prep from,flushed
R6737 T22701 T22702 det the,oviducts
R6738 T22702 T22700 pobj oviducts,from
R6739 T22703 T22695 cc and,flushed
R6740 T22704 T22705 advmod then,digested
R6741 T22705 T22695 conj digested,flushed
R6742 T22706 T22705 prep with,digested
R6743 T22707 T22708 compound proteinase,K
R6744 T22708 T22706 pobj K,with
R6745 T22709 T22695 punct .,flushed
R6746 T22711 T22712 prep In,co-amplified
R6747 T22713 T22714 amod separate,segments
R6748 T22714 T22711 pobj segments,In
R6749 T22715 T22714 compound reactions,segments
R6750 T22716 T22714 acl found,segments
R6751 T22717 T22716 advmod exclusively,found
R6752 T22718 T22716 prep in,found
R6753 T22719 T22720 preconj either,type
R6754 T22720 T22718 pobj type,in
R6755 T22721 T22720 det the,type
R6756 T22722 T22720 amod wild,type
R6757 T22723 T22720 punct -,type
R6758 T22724 T22720 cc or,type
R6759 T22725 T22720 conj mutant,type
R6760 T22726 T22712 nsubjpass alleles,co-amplified
R6761 T22727 T22712 auxpass were,co-amplified
R6762 T22728 T22712 prep with,co-amplified
R6763 T22729 T22730 det an,sequence
R6764 T22730 T22728 pobj sequence,with
R6765 T22731 T22730 amod internal,sequence
R6766 T22732 T22730 compound control,sequence
R6767 T22733 T22730 punct ", ",sequence
R6768 T22734 T22730 acl located,sequence
R6769 T22735 T22734 prep in,located
R6770 T22736 T22737 det the,arm
R6771 T22737 T22735 pobj arm,in
R6772 T22738 T22737 amod short,arm
R6773 T22739 T22737 punct (,arm
R6774 T22740 T22737 amod upstream,arm
R6775 T22741 T22737 punct ),arm
R6776 T22742 T22737 prep of,arm
R6777 T22743 T22744 det the,vector
R6778 T22744 T22742 pobj vector,of
R6779 T22745 T22744 compound targeting,vector
R6780 T22746 T22744 punct ", ",vector
R6781 T22747 T22748 dep which,found
R6782 T22748 T22744 relcl found,vector
R6783 T22749 T22748 auxpass is,found
R6784 T22750 T22748 prep in,found
R6785 T22751 T22752 det both,alleles
R6786 T22752 T22750 pobj alleles,in
R6787 T22753 T22712 punct .,co-amplified
R6788 T22755 T22756 det The,products
R6789 T22756 T22758 nsubj products,were
R6790 T22757 T22756 amod final,products
R6791 T22759 T22760 nummod 429,bp
R6792 T22760 T22758 attr bp,were
R6793 T22761 T22758 prep for,were
R6794 T22762 T22763 det the,allele
R6795 T22763 T22761 pobj allele,for
R6796 T22764 T22765 amod wild,type
R6797 T22765 T22763 compound type,allele
R6798 T22766 T22765 punct -,type
R6799 T22767 T22758 punct ", ",were
R6800 T22768 T22769 nummod 389,bp
R6801 T22769 T22758 conj bp,were
R6802 T22770 T22769 prep for,bp
R6803 T22771 T22772 det the,allele
R6804 T22772 T22770 pobj allele,for
R6805 T22773 T22772 compound mutant,allele
R6806 T22774 T22769 punct ", ",bp
R6807 T22775 T22769 cc and,bp
R6808 T22776 T22777 nummod 213,bp
R6809 T22777 T22769 conj bp,bp
R6810 T22778 T22777 prep for,bp
R6811 T22779 T22780 det the,control
R6812 T22780 T22778 pobj control,for
R6813 T22781 T22780 amod internal,control
R6814 T22782 T22758 punct .,were
R6815 T22784 T22785 punct (,A
R6816 T22785 T22786 meta A,example
R6817 T22787 T22785 punct ),A
R6818 T22788 T22786 det A,example
R6819 T22789 T22786 amod representative,example
R6820 T22790 T22786 prep of,example
R6821 T22791 T22792 det the,genotyping
R6822 T22792 T22790 pobj genotyping,of
R6823 T22793 T22792 prep of,genotyping
R6824 T22794 T22795 compound blastocyst,embryos
R6825 T22795 T22793 pobj embryos,of
R6826 T22796 T22795 compound stage,embryos
R6827 T22797 T22786 punct .,example
R6828 T22799 T22800 nmod Embryos,1
R6829 T22800 T22802 nsubj 1,were
R6830 T22801 T22800 punct #,1
R6831 T22803 T22800 punct ", ",1
R6832 T22804 T22800 conj 2,1
R6833 T22805 T22804 punct ", ",2
R6834 T22806 T22804 conj 4,2
R6835 T22807 T22806 punct ", ",4
R6836 T22808 T22806 conj 5,4
R6837 T22809 T22808 punct ", ",5
R6838 T22810 T22808 cc and,5
R6839 T22811 T22808 conj 6,5
R6840 T22812 T22802 attr Capn2,were
R6841 T22813 T22812 punct +,Capn2
R6842 T22814 T22812 punct /,Capn2
R6843 T22815 T22812 punct -,Capn2
R6844 T22816 T22817 mark whereas,were
R6845 T22817 T22802 advcl were,were
R6846 T22818 T22819 nmod embryos,3
R6847 T22819 T22817 nsubj 3,were
R6848 T22820 T22819 punct #,3
R6849 T22821 T22819 cc and,3
R6850 T22822 T22823 punct #,7
R6851 T22823 T22819 conj 7,3
R6852 T22824 T22817 attr Capn2,were
R6853 T22825 T22824 punct +,Capn2
R6854 T22826 T22824 punct /,Capn2
R6855 T22827 T22824 punct +,Capn2
R6856 T22828 T22817 punct ", ",were
R6857 T22829 T22817 advcl denoted,were
R6858 T22830 T22829 agent by,denoted
R6859 T22831 T22832 det the,absence
R6860 T22832 T22830 pobj absence,by
R6861 T22833 T22832 prep of,absence
R6862 T22834 T22835 det the,signal
R6863 T22835 T22833 pobj signal,of
R6864 T22836 T22837 nummod 389,bp
R6865 T22837 T22835 compound bp,signal
R6866 T22838 T22835 compound mutant,signal
R6867 T22839 T22802 punct .,were
R6868 T22841 T22842 punct (,B
R6869 T22842 T22843 meta B,shown
R6870 T22844 T22842 punct ),B
R6871 T22845 T22846 det An,example
R6872 T22846 T22843 nsubjpass example,shown
R6873 T22847 T22846 prep of,example
R6874 T22848 T22849 det the,genotyping
R6875 T22849 T22847 pobj genotyping,of
R6876 T22850 T22849 prep of,genotyping
R6877 T22851 T22852 nummod 8,cell
R6878 T22852 T22854 compound cell,embryos
R6879 T22853 T22852 punct -,cell
R6880 T22854 T22850 pobj embryos,of
R6881 T22855 T22843 auxpass is,shown
R6882 T22856 T22843 punct .,shown
R6883 T22858 T22859 nmod Embryos,1
R6884 T22859 T22861 nsubj 1,were
R6885 T22860 T22859 punct #,1
R6886 T22862 T22859 punct ", ",1
R6887 T22863 T22859 conj 2,1
R6888 T22864 T22863 punct ", ",2
R6889 T22865 T22863 conj 3,2
R6890 T22866 T22865 punct ", ",3
R6891 T22867 T22865 conj 5,3
R6892 T22868 T22867 punct ", ",5
R6893 T22869 T22867 cc and,5
R6894 T22870 T22867 conj 6,5
R6895 T22871 T22861 attr Capn2,were
R6896 T22872 T22871 punct +,Capn2
R6897 T22873 T22871 punct /,Capn2
R6898 T22874 T22871 punct -,Capn2
R6899 T22875 T22876 mark while,was
R6900 T22876 T22861 advcl was,were
R6901 T22877 T22876 nsubj embryo,was
R6902 T22878 T22877 punct #,embryo
R6903 T22879 T22877 nummod 4,embryo
R6904 T22880 T22876 attr Capn2,was
R6905 T22881 T22880 punct -,Capn2
R6906 T22882 T22880 punct /,Capn2
R6907 T22883 T22880 punct -,Capn2
R6908 T22884 T22880 punct ", ",Capn2
R6909 T22885 T22880 acl marked,Capn2
R6910 T22886 T22885 agent by,marked
R6911 T22887 T22888 det the,absence
R6912 T22888 T22886 pobj absence,by
R6913 T22889 T22888 prep of,absence
R6914 T22890 T22891 det the,signal
R6915 T22891 T22889 pobj signal,of
R6916 T22892 T22893 nummod 429,bp
R6917 T22893 T22891 nmod bp,signal
R6918 T22894 T22895 amod wild,type
R6919 T22895 T22891 compound type,signal
R6920 T22896 T22895 punct -,type
R6921 T22897 T22861 punct .,were
R6922 T22899 T22900 punct (,M
R6923 T22900 T22901 nsubj M,denotes
R6924 T22902 T22900 punct ),M
R6925 T22903 T22904 det the,marker
R6926 T22904 T22901 dobj marker,denotes
R6927 T22905 T22906 amod molecular,weight
R6928 T22906 T22904 compound weight,marker
R6929 T22907 T22901 punct .,denotes