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PMC:1382200 / 11892-13046 JSONTXT 4 Projects

Annnotations TAB TSV DIC JSON TextAE

Id Subject Object Predicate Lexical cue
T21857 10-16 VBN denotes Nested
T21859 17-20 NN denotes PCR
T21858 21-29 NN denotes strategy
T21860 30-33 IN denotes for
T21861 34-44 NN denotes genotyping
T21862 45-47 IN denotes of
T21863 48-64 JJ denotes pre-implantation
T21864 65-72 NNS denotes embryos
T21865 72-73 . denotes .
T21866 73-209 sentence denotes Due to the scarcity of extractable genetic material, a nested PCR strategy was developed in order to genotype pre-implantation embryos.
T21867 74-77 IN denotes Due
T21869 78-80 IN denotes to
T21870 81-84 DT denotes the
T21871 85-93 NN denotes scarcity
T21872 94-96 IN denotes of
T21873 97-108 JJ denotes extractable
T21875 109-116 JJ denotes genetic
T21874 117-125 NN denotes material
T21876 125-127 , denotes ,
T21877 127-128 DT denotes a
T21879 129-135 VBN denotes nested
T21880 136-139 NN denotes PCR
T21878 140-148 NN denotes strategy
T21881 149-152 VBD denotes was
T21868 153-162 VBN denotes developed
T21882 163-165 IN denotes in
T21883 166-171 NN denotes order
T21884 172-174 TO denotes to
T21885 175-183 VB denotes genotype
T21886 184-200 JJ denotes pre-implantation
T21887 201-208 NNS denotes embryos
T21888 208-209 . denotes .
T21889 209-416 sentence denotes Separate reactions were used to amplify a 429 bp fragment from the wild-type allele and a 389 bp segment from the mutant allele, both spanning the 3' end of the short (upstream) arm of the targeting vector.
T21890 210-218 JJ denotes Separate
T21891 219-228 NNS denotes reactions
T21893 229-233 VBD denotes were
T21892 234-238 VBN denotes used
T21894 239-241 TO denotes to
T21895 242-249 VB denotes amplify
T21896 250-251 DT denotes a
T21898 252-255 CD denotes 429
T21899 256-258 NN denotes bp
T21897 259-267 NN denotes fragment
T21900 268-272 IN denotes from
T21901 273-276 DT denotes the
T21903 277-281 JJ denotes wild
T21905 281-282 HYPH denotes -
T21904 282-286 NN denotes type
T21902 287-293 NN denotes allele
T21906 294-297 CC denotes and
T21907 298-299 DT denotes a
T21909 300-303 CD denotes 389
T21910 304-306 NN denotes bp
T21908 307-314 NN denotes segment
T21911 315-319 IN denotes from
T21912 320-323 DT denotes the
T21914 324-330 NN denotes mutant
T21913 331-337 NN denotes allele
T21915 337-339 , denotes ,
T21916 339-343 DT denotes both
T21917 344-352 VBG denotes spanning
T21918 353-356 DT denotes the
T21920 357-358 CD denotes 3
T21921 358-359 SYM denotes '
T21919 360-363 NN denotes end
T21922 364-366 IN denotes of
T21923 367-370 DT denotes the
T21925 371-376 JJ denotes short
T21926 377-378 -LRB- denotes (
T21927 378-386 JJ denotes upstream
T21928 386-387 -RRB- denotes )
T21924 388-391 NN denotes arm
T21929 392-394 IN denotes of
T21930 395-398 DT denotes the
T21932 399-408 NN denotes targeting
T21931 409-415 NN denotes vector
T21933 415-416 . denotes .
T21934 416-554 sentence denotes In both reactions, a 213 bp sequence located within the short arm was co-amplified with the 'diagnostic' products as an internal control.
T21935 417-419 IN denotes In
T21937 420-424 DT denotes both
T21938 425-434 NNS denotes reactions
T21939 434-436 , denotes ,
T21940 436-437 DT denotes a
T21942 438-441 CD denotes 213
T21943 442-444 NN denotes bp
T21941 445-453 NN denotes sequence
T21944 454-461 VBN denotes located
T21945 462-468 IN denotes within
T21946 469-472 DT denotes the
T21948 473-478 JJ denotes short
T21947 479-482 NN denotes arm
T21949 483-486 VBD denotes was
T21936 487-499 VBN denotes co-amplified
T21950 500-504 IN denotes with
T21951 505-508 DT denotes the
T21953 509-510 `` denotes '
T21954 510-520 JJ denotes diagnostic
T21955 520-521 '' denotes '
T21952 522-530 NNS denotes products
T21956 531-533 IN denotes as
T21957 534-536 DT denotes an
T21959 537-545 JJ denotes internal
T21958 546-553 NN denotes control
T21960 553-554 . denotes .
T21961 554-692 sentence denotes The same sense primers were used to amplify 'diagnostic' sequences in both reactions, whereas the antisense primers were allele-specific.
T21962 555-558 DT denotes The
T21964 559-563 JJ denotes same
T21965 564-569 NN denotes sense
T21963 570-577 NNS denotes primers
T21967 578-582 VBD denotes were
T21966 583-587 VBN denotes used
T21968 588-590 TO denotes to
T21969 591-598 VB denotes amplify
T21970 599-600 `` denotes '
T21971 600-610 JJ denotes diagnostic
T21973 610-611 '' denotes '
T21972 612-621 NNS denotes sequences
T21974 622-624 IN denotes in
T21975 625-629 DT denotes both
T21976 630-639 NNS denotes reactions
T21977 639-641 , denotes ,
T21978 641-648 IN denotes whereas
T21980 649-652 DT denotes the
T21982 653-662 NN denotes antisense
T21981 663-670 NNS denotes primers
T21979 671-675 VBD denotes were
T21983 676-682 NN denotes allele
T21985 682-683 HYPH denotes -
T21984 683-691 JJ denotes specific
T21986 691-692 . denotes .
T21987 692-798 sentence denotes The primers, represented by triangles, are depicted in two (nested) sets for each of the three reactions.
T21988 693-696 DT denotes The
T21989 697-704 NNS denotes primers
T21991 704-706 , denotes ,
T21992 706-717 VBN denotes represented
T21993 718-720 IN denotes by
T21994 721-730 NNS denotes triangles
T21995 730-732 , denotes ,
T21996 732-735 VBP denotes are
T21990 736-744 VBN denotes depicted
T21997 745-747 IN denotes in
T21998 748-751 CD denotes two
T22000 752-753 -LRB- denotes (
T22001 753-759 VBN denotes nested
T22002 759-760 -RRB- denotes )
T21999 761-765 NNS denotes sets
T22003 766-769 IN denotes for
T22004 770-774 DT denotes each
T22005 775-777 IN denotes of
T22006 778-781 DT denotes the
T22008 782-787 CD denotes three
T22007 788-797 NNS denotes reactions
T22009 797-798 . denotes .
T22010 798-1015 sentence denotes Exons are represented by open, vertical rectangles, the PGK-Neo cassette by an open, horizontal rectangle, while thin vertical lines denote the boundaries of the short arm and the 5' end of the long (downstream) arm.
T22011 799-804 NNS denotes Exons
T22013 805-808 VBP denotes are
T22012 809-820 VBN denotes represented
T22015 821-823 IN denotes by
T22016 824-828 JJ denotes open
T22018 828-830 , denotes ,
T22019 830-838 JJ denotes vertical
T22017 839-849 NNS denotes rectangles
T22020 849-851 , denotes ,
T22021 851-854 DT denotes the
T22022 855-858 NN denotes PGK
T22024 858-859 HYPH denotes -
T22023 859-862 NN denotes Neo
T22014 863-871 NN denotes cassette
T22025 872-874 IN denotes by
T22026 875-877 DT denotes an
T22028 878-882 JJ denotes open
T22029 882-884 , denotes ,
T22030 884-894 JJ denotes horizontal
T22027 895-904 NN denotes rectangle
T22031 904-906 , denotes ,
T22032 906-911 IN denotes while
T22034 912-916 JJ denotes thin
T22036 917-925 JJ denotes vertical
T22035 926-931 NNS denotes lines
T22033 932-938 VBP denotes denote
T22037 939-942 DT denotes the
T22038 943-953 NNS denotes boundaries
T22039 954-956 IN denotes of
T22040 957-960 DT denotes the
T22042 961-966 JJ denotes short
T22041 967-970 NN denotes arm
T22043 971-974 CC denotes and
T22044 975-978 DT denotes the
T22046 979-980 CD denotes 5
T22047 980-981 SYM denotes '
T22045 982-985 NN denotes end
T22048 986-988 IN denotes of
T22049 989-992 DT denotes the
T22051 993-997 JJ denotes long
T22052 998-999 -LRB- denotes (
T22053 999-1009 JJ denotes downstream
T22054 1009-1010 -RRB- denotes )
T22050 1011-1014 NN denotes arm
T22055 1014-1015 . denotes .
T22056 1015-1154 sentence denotes A grey, horizontal rectangle delineates the segment of the wild-type allele that is replaced by the PGK-Neo cassette in the mutant allele.
T22057 1016-1017 DT denotes A
T22059 1018-1022 JJ denotes grey
T22060 1022-1024 , denotes ,
T22061 1024-1034 JJ denotes horizontal
T22058 1035-1044 NN denotes rectangle
T22062 1045-1055 VBZ denotes delineates
T22063 1056-1059 DT denotes the
T22064 1060-1067 NN denotes segment
T22065 1068-1070 IN denotes of
T22066 1071-1074 DT denotes the
T22068 1075-1079 JJ denotes wild
T22070 1079-1080 HYPH denotes -
T22069 1080-1084 NN denotes type
T22067 1085-1091 NN denotes allele
T22071 1092-1096 WDT denotes that
T22073 1097-1099 VBZ denotes is
T22072 1100-1108 VBN denotes replaced
T22074 1109-1111 IN denotes by
T22075 1112-1115 DT denotes the
T22077 1116-1119 NN denotes PGK
T22079 1119-1120 HYPH denotes -
T22078 1120-1123 NN denotes Neo
T22076 1124-1132 NN denotes cassette
T22080 1133-1135 IN denotes in
T22081 1136-1139 DT denotes the
T22083 1140-1146 NN denotes mutant
T22082 1147-1153 NN denotes allele
T22084 1153-1154 . denotes .
R6470 T21857 T21858 amod Nested,strategy
R6471 T21859 T21858 compound PCR,strategy
R6472 T21860 T21858 prep for,strategy
R6473 T21861 T21860 pobj genotyping,for
R6474 T21862 T21861 prep of,genotyping
R6475 T21863 T21864 amod pre-implantation,embryos
R6476 T21864 T21862 pobj embryos,of
R6477 T21865 T21858 punct .,strategy
R6478 T21867 T21868 prep Due,developed
R6479 T21869 T21867 pcomp to,Due
R6480 T21870 T21871 det the,scarcity
R6481 T21871 T21867 pobj scarcity,Due
R6482 T21872 T21871 prep of,scarcity
R6483 T21873 T21874 amod extractable,material
R6484 T21874 T21872 pobj material,of
R6485 T21875 T21874 amod genetic,material
R6486 T21876 T21868 punct ", ",developed
R6487 T21877 T21878 det a,strategy
R6488 T21878 T21868 nsubjpass strategy,developed
R6489 T21879 T21878 amod nested,strategy
R6490 T21880 T21878 compound PCR,strategy
R6491 T21881 T21868 auxpass was,developed
R6492 T21882 T21868 prep in,developed
R6493 T21883 T21882 pobj order,in
R6494 T21884 T21885 aux to,genotype
R6495 T21885 T21883 acl genotype,order
R6496 T21886 T21887 amod pre-implantation,embryos
R6497 T21887 T21885 dobj embryos,genotype
R6498 T21888 T21868 punct .,developed
R6499 T21890 T21891 amod Separate,reactions
R6500 T21891 T21892 nsubjpass reactions,used
R6501 T21893 T21892 auxpass were,used
R6502 T21894 T21895 aux to,amplify
R6503 T21895 T21892 advcl amplify,used
R6504 T21896 T21897 det a,fragment
R6505 T21897 T21895 dobj fragment,amplify
R6506 T21898 T21899 nummod 429,bp
R6507 T21899 T21897 compound bp,fragment
R6508 T21900 T21897 prep from,fragment
R6509 T21901 T21902 det the,allele
R6510 T21902 T21900 pobj allele,from
R6511 T21903 T21904 amod wild,type
R6512 T21904 T21902 compound type,allele
R6513 T21905 T21904 punct -,type
R6514 T21906 T21897 cc and,fragment
R6515 T21907 T21908 det a,segment
R6516 T21908 T21897 conj segment,fragment
R6517 T21909 T21910 nummod 389,bp
R6518 T21910 T21908 compound bp,segment
R6519 T21911 T21908 prep from,segment
R6520 T21912 T21913 det the,allele
R6521 T21913 T21911 pobj allele,from
R6522 T21914 T21913 compound mutant,allele
R6523 T21915 T21897 punct ", ",fragment
R6524 T21916 T21897 appos both,fragment
R6525 T21917 T21916 acl spanning,both
R6526 T21918 T21919 det the,end
R6527 T21919 T21917 dobj end,spanning
R6528 T21920 T21919 nummod 3,end
R6529 T21921 T21920 punct ',3
R6530 T21922 T21919 prep of,end
R6531 T21923 T21924 det the,arm
R6532 T21924 T21922 pobj arm,of
R6533 T21925 T21924 amod short,arm
R6534 T21926 T21927 punct (,upstream
R6535 T21927 T21924 parataxis upstream,arm
R6536 T21928 T21927 punct ),upstream
R6537 T21929 T21924 prep of,arm
R6538 T21930 T21931 det the,vector
R6539 T21931 T21929 pobj vector,of
R6540 T21932 T21931 compound targeting,vector
R6541 T21933 T21892 punct .,used
R6542 T21935 T21936 prep In,co-amplified
R6543 T21937 T21938 det both,reactions
R6544 T21938 T21935 pobj reactions,In
R6545 T21939 T21936 punct ", ",co-amplified
R6546 T21940 T21941 det a,sequence
R6547 T21941 T21936 nsubjpass sequence,co-amplified
R6548 T21942 T21943 nummod 213,bp
R6549 T21943 T21941 compound bp,sequence
R6550 T21944 T21941 acl located,sequence
R6551 T21945 T21944 prep within,located
R6552 T21946 T21947 det the,arm
R6553 T21947 T21945 pobj arm,within
R6554 T21948 T21947 amod short,arm
R6555 T21949 T21936 auxpass was,co-amplified
R6556 T21950 T21936 prep with,co-amplified
R6557 T21951 T21952 det the,products
R6558 T21952 T21950 pobj products,with
R6559 T21953 T21952 punct ',products
R6560 T21954 T21952 amod diagnostic,products
R6561 T21955 T21952 punct ',products
R6562 T21956 T21936 prep as,co-amplified
R6563 T21957 T21958 det an,control
R6564 T21958 T21956 pobj control,as
R6565 T21959 T21958 amod internal,control
R6566 T21960 T21936 punct .,co-amplified
R6567 T21962 T21963 det The,primers
R6568 T21963 T21966 nsubjpass primers,used
R6569 T21964 T21963 amod same,primers
R6570 T21965 T21963 compound sense,primers
R6571 T21967 T21966 auxpass were,used
R6572 T21968 T21969 aux to,amplify
R6573 T21969 T21966 advcl amplify,used
R6574 T21970 T21969 punct ',amplify
R6575 T21971 T21972 amod diagnostic,sequences
R6576 T21972 T21969 dobj sequences,amplify
R6577 T21973 T21972 punct ',sequences
R6578 T21974 T21972 prep in,sequences
R6579 T21975 T21976 det both,reactions
R6580 T21976 T21974 pobj reactions,in
R6581 T21977 T21969 punct ", ",amplify
R6582 T21978 T21979 mark whereas,were
R6583 T21979 T21969 advcl were,amplify
R6584 T21980 T21981 det the,primers
R6585 T21981 T21979 nsubj primers,were
R6586 T21982 T21981 compound antisense,primers
R6587 T21983 T21984 npadvmod allele,specific
R6588 T21984 T21979 acomp specific,were
R6589 T21985 T21984 punct -,specific
R6590 T21986 T21966 punct .,used
R6591 T21988 T21989 det The,primers
R6592 T21989 T21990 nsubjpass primers,depicted
R6593 T21991 T21989 punct ", ",primers
R6594 T21992 T21989 acl represented,primers
R6595 T21993 T21992 agent by,represented
R6596 T21994 T21993 pobj triangles,by
R6597 T21995 T21990 punct ", ",depicted
R6598 T21996 T21990 auxpass are,depicted
R6599 T21997 T21990 prep in,depicted
R6600 T21998 T21999 nummod two,sets
R6601 T21999 T21997 pobj sets,in
R6602 T22000 T21999 punct (,sets
R6603 T22001 T21999 amod nested,sets
R6604 T22002 T21999 punct ),sets
R6605 T22003 T21999 prep for,sets
R6606 T22004 T22003 pobj each,for
R6607 T22005 T22004 prep of,each
R6608 T22006 T22007 det the,reactions
R6609 T22007 T22005 pobj reactions,of
R6610 T22008 T22007 nummod three,reactions
R6611 T22009 T21990 punct .,depicted
R6612 T22011 T22012 nsubjpass Exons,represented
R6613 T22012 T22014 ccomp represented,cassette
R6614 T22013 T22012 auxpass are,represented
R6615 T22015 T22012 agent by,represented
R6616 T22016 T22017 amod open,rectangles
R6617 T22017 T22015 pobj rectangles,by
R6618 T22018 T22017 punct ", ",rectangles
R6619 T22019 T22017 amod vertical,rectangles
R6620 T22020 T22014 punct ", ",cassette
R6621 T22021 T22014 det the,cassette
R6622 T22022 T22023 compound PGK,Neo
R6623 T22023 T22014 compound Neo,cassette
R6624 T22024 T22023 punct -,Neo
R6625 T22025 T22014 agent by,cassette
R6626 T22026 T22027 det an,rectangle
R6627 T22027 T22025 pobj rectangle,by
R6628 T22028 T22027 amod open,rectangle
R6629 T22029 T22027 punct ", ",rectangle
R6630 T22030 T22027 amod horizontal,rectangle
R6631 T22031 T22014 punct ", ",cassette
R6632 T22032 T22033 mark while,denote
R6633 T22033 T22014 advcl denote,cassette
R6634 T22034 T22035 amod thin,lines
R6635 T22035 T22033 nsubj lines,denote
R6636 T22036 T22035 amod vertical,lines
R6637 T22037 T22038 det the,boundaries
R6638 T22038 T22033 dobj boundaries,denote
R6639 T22039 T22038 prep of,boundaries
R6640 T22040 T22041 det the,arm
R6641 T22041 T22039 pobj arm,of
R6642 T22042 T22041 amod short,arm
R6643 T22043 T22038 cc and,boundaries
R6644 T22044 T22045 det the,end
R6645 T22045 T22038 conj end,boundaries
R6646 T22046 T22045 nummod 5,end
R6647 T22047 T22046 punct ',5
R6648 T22048 T22045 prep of,end
R6649 T22049 T22050 det the,arm
R6650 T22050 T22048 pobj arm,of
R6651 T22051 T22050 amod long,arm
R6652 T22052 T22050 punct (,arm
R6653 T22053 T22050 amod downstream,arm
R6654 T22054 T22050 punct ),arm
R6655 T22055 T22014 punct .,cassette
R6656 T22057 T22058 det A,rectangle
R6657 T22058 T22062 nsubj rectangle,delineates
R6658 T22059 T22058 amod grey,rectangle
R6659 T22060 T22058 punct ", ",rectangle
R6660 T22061 T22058 amod horizontal,rectangle
R6661 T22063 T22064 det the,segment
R6662 T22064 T22062 dobj segment,delineates
R6663 T22065 T22064 prep of,segment
R6664 T22066 T22067 det the,allele
R6665 T22067 T22065 pobj allele,of
R6666 T22068 T22069 amod wild,type
R6667 T22069 T22067 compound type,allele
R6668 T22070 T22069 punct -,type
R6669 T22071 T22072 dep that,replaced
R6670 T22072 T22064 relcl replaced,segment
R6671 T22073 T22072 auxpass is,replaced
R6672 T22074 T22072 agent by,replaced
R6673 T22075 T22076 det the,cassette
R6674 T22076 T22074 pobj cassette,by
R6675 T22077 T22078 compound PGK,Neo
R6676 T22078 T22076 compound Neo,cassette
R6677 T22079 T22078 punct -,Neo
R6678 T22080 T22072 prep in,replaced
R6679 T22081 T22082 det the,allele
R6680 T22082 T22080 pobj allele,in
R6681 T22083 T22082 compound mutant,allele
R6682 T22084 T22062 punct .,delineates