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PMC:1189073 / 35756-36148 JSONTXT 4 Projects

Annnotations TAB TSV DIC JSON TextAE

Id Subject Object Predicate Lexical cue
T17905 0-15 NN denotes Endoglycosidase
T17906 16-25 NN denotes digestion
T17907 25-26 . denotes .
T17908 26-182 sentence denotes Kidney membranes (60 μg) were incubated in 50 mM sodium phosphate (pH 5.5), 0.1% SDS, and 50 mM β-mercaptoethanol, heated to 100 °C for 5 min, then cooled.
T17909 27-33 NN denotes Kidney
T17910 34-43 NNS denotes membranes
T17912 44-45 -LRB- denotes (
T17914 45-47 CD denotes 60
T17913 48-50 NN denotes μg
T17915 50-51 -RRB- denotes )
T17916 52-56 VBD denotes were
T17911 57-66 VBN denotes incubated
T17917 67-69 IN denotes in
T17918 70-72 CD denotes 50
T17919 73-75 NN denotes mM
T17921 76-82 NN denotes sodium
T17920 83-92 NN denotes phosphate
T17922 93-94 -LRB- denotes (
T17923 94-96 NN denotes pH
T17924 97-100 CD denotes 5.5
T17925 100-101 -RRB- denotes )
T17926 101-103 , denotes ,
T17927 103-106 CD denotes 0.1
T17928 106-107 NN denotes %
T17929 108-111 NN denotes SDS
T17930 111-113 , denotes ,
T17931 113-116 CC denotes and
T17932 117-119 CD denotes 50
T17933 120-122 NN denotes mM
T17935 123-124 NN denotes β
T17936 124-125 HYPH denotes -
T17934 125-140 NN denotes mercaptoethanol
T17937 140-142 , denotes ,
T17938 142-148 VBN denotes heated
T17939 149-151 IN denotes to
T17940 152-155 CD denotes 100
T17941 156-158 NN denotes °C
T17942 159-162 IN denotes for
T17943 163-164 CD denotes 5
T17944 165-168 NN denotes min
T17945 168-170 , denotes ,
T17946 170-174 RB denotes then
T17947 175-181 VBN denotes cooled
T17948 181-182 . denotes .
T17949 182-270 sentence denotes Endoglycosidase H (0.01 units; Sigma-Aldrich) was added and incubated at 37 °C for 2 h.
T17950 183-198 NN denotes Endoglycosidase
T17951 199-200 NN denotes H
T17953 201-202 -LRB- denotes (
T17955 202-206 CD denotes 0.01
T17956 207-212 NNS denotes units
T17957 212-213 : denotes ;
T17958 214-219 NNP denotes Sigma
T17959 219-220 HYPH denotes -
T17954 220-227 NNP denotes Aldrich
T17960 227-228 -RRB- denotes )
T17961 229-232 VBD denotes was
T17952 233-238 VBN denotes added
T17962 239-242 CC denotes and
T17963 243-252 VBN denotes incubated
T17964 253-255 IN denotes at
T17965 256-258 CD denotes 37
T17966 259-261 NN denotes °C
T17967 262-265 IN denotes for
T17968 266-267 CD denotes 2
T17969 268-269 NN denotes h
T17970 269-270 . denotes .
T17971 270-337 sentence denotes The reaction was stopped by boiling the samples in Laemmli buffer.
T17972 271-274 DT denotes The
T17973 275-283 NN denotes reaction
T17975 284-287 VBD denotes was
T17974 288-295 VBN denotes stopped
T17976 296-298 IN denotes by
T17977 299-306 VBG denotes boiling
T17978 307-310 DT denotes the
T17979 311-318 NNS denotes samples
T17980 319-321 IN denotes in
T17981 322-329 NNP denotes Laemmli
T17982 330-336 NN denotes buffer
T17983 336-337 . denotes .
T17984 337-392 sentence denotes Total reactants were immunoblotted as described above.
T17985 338-343 JJ denotes Total
T17986 344-353 NNS denotes reactants
T17988 354-358 VBD denotes were
T17987 359-372 VBN denotes immunoblotted
T17989 373-375 IN denotes as
T17990 376-385 VBN denotes described
T17991 386-391 RB denotes above
T17992 391-392 . denotes .
R5213 T17905 T17906 compound Endoglycosidase,digestion
R5214 T17907 T17906 punct .,digestion
R5215 T17909 T17910 compound Kidney,membranes
R5216 T17910 T17911 nsubjpass membranes,incubated
R5217 T17912 T17913 punct (,μg
R5218 T17913 T17910 parataxis μg,membranes
R5219 T17914 T17913 nummod 60,μg
R5220 T17915 T17913 punct ),μg
R5221 T17916 T17911 auxpass were,incubated
R5222 T17917 T17911 prep in,incubated
R5223 T17918 T17919 nummod 50,mM
R5224 T17919 T17920 compound mM,phosphate
R5225 T17920 T17917 pobj phosphate,in
R5226 T17921 T17920 compound sodium,phosphate
R5227 T17922 T17920 punct (,phosphate
R5228 T17923 T17920 npadvmod pH,phosphate
R5229 T17924 T17923 nummod 5.5,pH
R5230 T17925 T17920 punct ),phosphate
R5231 T17926 T17920 punct ", ",phosphate
R5232 T17927 T17928 nummod 0.1,%
R5233 T17928 T17929 compound %,SDS
R5234 T17929 T17920 appos SDS,phosphate
R5235 T17930 T17929 punct ", ",SDS
R5236 T17931 T17929 cc and,SDS
R5237 T17932 T17933 nummod 50,mM
R5238 T17933 T17934 compound mM,mercaptoethanol
R5239 T17934 T17929 conj mercaptoethanol,SDS
R5240 T17935 T17934 compound β,mercaptoethanol
R5241 T17936 T17934 punct -,mercaptoethanol
R5242 T17937 T17911 punct ", ",incubated
R5243 T17938 T17911 dep heated,incubated
R5244 T17939 T17938 prep to,heated
R5245 T17940 T17941 nummod 100,°C
R5246 T17941 T17939 pobj °C,to
R5247 T17942 T17938 prep for,heated
R5248 T17943 T17944 nummod 5,min
R5249 T17944 T17942 pobj min,for
R5250 T17945 T17911 punct ", ",incubated
R5251 T17946 T17947 advmod then,cooled
R5252 T17947 T17911 dep cooled,incubated
R5253 T17948 T17911 punct .,incubated
R5254 T17950 T17951 compound Endoglycosidase,H
R5255 T17951 T17952 nsubjpass H,added
R5256 T17953 T17954 punct (,Aldrich
R5257 T17954 T17951 parataxis Aldrich,H
R5258 T17955 T17956 nummod 0.01,units
R5259 T17956 T17954 dep units,Aldrich
R5260 T17957 T17954 punct ;,Aldrich
R5261 T17958 T17954 compound Sigma,Aldrich
R5262 T17959 T17954 punct -,Aldrich
R5263 T17960 T17954 punct ),Aldrich
R5264 T17961 T17952 auxpass was,added
R5265 T17962 T17952 cc and,added
R5266 T17963 T17952 conj incubated,added
R5267 T17964 T17963 prep at,incubated
R5268 T17965 T17966 nummod 37,°C
R5269 T17966 T17964 pobj °C,at
R5270 T17967 T17963 prep for,incubated
R5271 T17968 T17969 nummod 2,h
R5272 T17969 T17967 pobj h,for
R5273 T17970 T17952 punct .,added
R5274 T17972 T17973 det The,reaction
R5275 T17973 T17974 nsubjpass reaction,stopped
R5276 T17975 T17974 auxpass was,stopped
R5277 T17976 T17974 prep by,stopped
R5278 T17977 T17976 pcomp boiling,by
R5279 T17978 T17979 det the,samples
R5280 T17979 T17977 dobj samples,boiling
R5281 T17980 T17977 prep in,boiling
R5282 T17981 T17982 compound Laemmli,buffer
R5283 T17982 T17980 pobj buffer,in
R5284 T17983 T17974 punct .,stopped
R5285 T17985 T17986 amod Total,reactants
R5286 T17986 T17987 nsubjpass reactants,immunoblotted
R5287 T17988 T17987 auxpass were,immunoblotted
R5288 T17989 T17990 mark as,described
R5289 T17990 T17987 advcl described,immunoblotted
R5290 T17991 T17990 advmod above,described
R5291 T17992 T17987 punct .,immunoblotted