craft-sa-dev Home  

PMC:1189073 / 3078-40999 JSONTXT 5 Projects

Annnotations TAB TSV DIC JSON TextAE

Id Subject Object Predicate Lexical cue
T2464 13-24 JJ denotes Nephrogenic
T2466 25-33 NN denotes diabetes
T2465 34-43 NN denotes insipidus
T2468 44-45 -LRB- denotes (
T2469 45-48 NN denotes NDI
T2470 48-49 -RRB- denotes )
T2467 50-52 VBZ denotes is
T2471 53-54 DT denotes a
T2472 55-62 NN denotes disease
T2473 63-76 VBN denotes characterized
T2474 77-79 IN denotes by
T2475 80-89 JJ denotes excessive
T2476 90-99 NN denotes urination
T2477 100-103 CC denotes and
T2478 104-110 RB denotes thirst
T2479 110-112 , denotes ,
T2480 112-119 IN denotes despite
T2481 120-126 JJ denotes normal
T2482 127-137 NN denotes production
T2483 138-140 IN denotes of
T2484 141-144 DT denotes the
T2486 145-157 JJ denotes antidiuretic
T2485 158-165 NN denotes hormone
T2487 166-174 NN denotes arginine
T2488 175-186 NN denotes vasopressin
T2489 187-188 -LRB- denotes (
T2490 188-191 NN denotes AVP
T2491 191-192 -RRB- denotes )
T2492 193-194 -LRB- denotes [
T2493 194-195 CD denotes 1
T2494 195-196 -RRB- denotes ]
T2495 196-197 . denotes .
T2496 197-340 sentence denotes The inherited forms are either X-linked as a consequence of mutation of the Avpr2 gene [2], or autosomal due to mutation of the Aqp2 gene [3].
T2497 198-201 DT denotes The
T2499 202-211 VBN denotes inherited
T2498 212-217 NNS denotes forms
T2500 218-221 VBP denotes are
T2501 222-228 RB denotes either
T2503 229-230 NN denotes X
T2504 230-231 HYPH denotes -
T2502 231-237 VBN denotes linked
T2505 238-240 IN denotes as
T2506 241-242 DT denotes a
T2507 243-254 NN denotes consequence
T2508 255-257 IN denotes of
T2509 258-266 NN denotes mutation
T2510 267-269 IN denotes of
T2511 270-273 DT denotes the
T2513 274-279 NN denotes Avpr2
T2512 280-284 NN denotes gene
T2514 285-286 -LRB- denotes [
T2515 286-287 CD denotes 2
T2516 287-288 -RRB- denotes ]
T2517 288-290 , denotes ,
T2518 290-292 CC denotes or
T2519 293-302 JJ denotes autosomal
T2520 303-306 JJ denotes due
T2521 307-309 IN denotes to
T2522 310-318 NN denotes mutation
T2523 319-321 IN denotes of
T2524 322-325 DT denotes the
T2526 326-330 NN denotes Aqp2
T2525 331-335 NN denotes gene
T2527 336-337 -LRB- denotes [
T2528 337-338 CD denotes 3
T2529 338-339 -RRB- denotes ]
T2530 339-340 . denotes .
T2531 340-504 sentence denotes Aquaporin-2 (AQP2) is a pore-forming protein belonging to a family of water channels [4], and it is expressed in collecting-duct principal cells in the kidney [5].
T2532 341-350 NN denotes Aquaporin
T2534 350-351 HYPH denotes -
T2535 351-352 CD denotes 2
T2536 353-354 -LRB- denotes (
T2537 354-358 NN denotes AQP2
T2538 358-359 -RRB- denotes )
T2533 360-362 VBZ denotes is
T2539 363-364 DT denotes a
T2541 365-369 NN denotes pore
T2543 369-370 HYPH denotes -
T2542 370-377 VBG denotes forming
T2540 378-385 NN denotes protein
T2544 386-395 VBG denotes belonging
T2545 396-398 IN denotes to
T2546 399-400 DT denotes a
T2547 401-407 NN denotes family
T2548 408-410 IN denotes of
T2549 411-416 NN denotes water
T2550 417-425 NNS denotes channels
T2551 426-427 -LRB- denotes [
T2552 427-428 CD denotes 4
T2553 428-429 -RRB- denotes ]
T2554 429-431 , denotes ,
T2555 431-434 CC denotes and
T2556 435-437 PRP denotes it
T2558 438-440 VBZ denotes is
T2557 441-450 VBN denotes expressed
T2559 451-453 IN denotes in
T2560 454-464 VBG denotes collecting
T2562 464-465 HYPH denotes -
T2561 465-469 NN denotes duct
T2564 470-479 JJ denotes principal
T2563 480-485 NNS denotes cells
T2565 486-488 IN denotes in
T2566 489-492 DT denotes the
T2567 493-499 NN denotes kidney
T2568 500-501 -LRB- denotes [
T2569 501-502 CD denotes 5
T2570 502-503 -RRB- denotes ]
T2571 503-504 . denotes .
T2572 504-713 sentence denotes Generally these proteins permit the passage of water through the plasma membrane (PM) of cells, several of which carry out this role specifically in the process of water reabsorption from urine in the kidney.
T2573 505-514 RB denotes Generally
T2575 515-520 DT denotes these
T2576 521-529 NN denotes proteins
T2574 530-536 VBP denotes permit
T2577 537-540 DT denotes the
T2578 541-548 NN denotes passage
T2579 549-551 IN denotes of
T2580 552-557 NN denotes water
T2581 558-565 IN denotes through
T2582 566-569 DT denotes the
T2584 570-576 NN denotes plasma
T2583 577-585 NN denotes membrane
T2585 586-587 -LRB- denotes (
T2586 587-589 NN denotes PM
T2587 589-590 -RRB- denotes )
T2588 591-593 IN denotes of
T2589 594-599 NNS denotes cells
T2590 599-601 , denotes ,
T2591 601-608 JJ denotes several
T2593 609-611 IN denotes of
T2594 612-617 WDT denotes which
T2592 618-623 VBP denotes carry
T2595 624-627 RP denotes out
T2596 628-632 DT denotes this
T2597 633-637 NN denotes role
T2598 638-650 RB denotes specifically
T2599 651-653 IN denotes in
T2600 654-657 DT denotes the
T2601 658-665 NN denotes process
T2602 666-668 IN denotes of
T2603 669-674 NN denotes water
T2604 675-687 NN denotes reabsorption
T2605 688-692 IN denotes from
T2606 693-698 NN denotes urine
T2607 699-701 IN denotes in
T2608 702-705 DT denotes the
T2609 706-712 NN denotes kidney
T2610 712-713 . denotes .
T2611 713-855 sentence denotes It has been established that aquaporins, although functional as a monomer, tetramerize before their insertion into the plasma membrane [4,6].
T2612 714-716 PRP denotes It
T2614 717-720 VBZ denotes has
T2615 721-725 VBN denotes been
T2613 726-737 VBN denotes established
T2616 738-742 IN denotes that
T2618 743-753 NNS denotes aquaporins
T2619 753-755 , denotes ,
T2620 755-763 IN denotes although
T2621 764-774 JJ denotes functional
T2622 775-777 IN denotes as
T2623 778-779 DT denotes a
T2624 780-787 NN denotes monomer
T2625 787-789 , denotes ,
T2617 789-800 VBP denotes tetramerize
T2626 801-807 IN denotes before
T2627 808-813 PRP$ denotes their
T2628 814-823 NN denotes insertion
T2629 824-828 IN denotes into
T2630 829-832 DT denotes the
T2632 833-839 NN denotes plasma
T2631 840-848 NN denotes membrane
T2633 849-850 -LRB- denotes [
T2635 850-851 CD denotes 4
T2636 851-852 , denotes ,
T2634 852-853 CD denotes 6
T2637 853-854 -RRB- denotes ]
T2638 854-855 . denotes .
T2639 855-1119 sentence denotes Furthermore these proteins can also be differentially targeted to distinct regions of the PM; for example, AQP2 is routed to the apical membrane of cells surrounding the collecting duct, whereas other aquaporins (AQP3 or 4) are inserted into the basolateral face.
T2640 856-867 RB denotes Furthermore
T2642 868-873 DT denotes these
T2643 874-882 NN denotes proteins
T2644 883-886 MD denotes can
T2645 887-891 RB denotes also
T2646 892-894 VB denotes be
T2647 895-909 RB denotes differentially
T2641 910-918 VBN denotes targeted
T2649 919-921 IN denotes to
T2650 922-930 JJ denotes distinct
T2651 931-938 NNS denotes regions
T2652 939-941 IN denotes of
T2653 942-945 DT denotes the
T2654 946-948 NN denotes PM
T2655 948-949 : denotes ;
T2656 950-953 IN denotes for
T2657 954-961 NN denotes example
T2658 961-963 , denotes ,
T2659 963-967 NN denotes AQP2
T2660 968-970 VBZ denotes is
T2648 971-977 VBN denotes routed
T2661 978-980 IN denotes to
T2662 981-984 DT denotes the
T2664 985-991 JJ denotes apical
T2663 992-1000 NN denotes membrane
T2665 1001-1003 IN denotes of
T2666 1004-1009 NNS denotes cells
T2667 1010-1021 VBG denotes surrounding
T2668 1022-1025 DT denotes the
T2670 1026-1036 VBG denotes collecting
T2669 1037-1041 NN denotes duct
T2671 1041-1043 , denotes ,
T2672 1043-1050 IN denotes whereas
T2674 1051-1056 JJ denotes other
T2675 1057-1067 NNS denotes aquaporins
T2676 1068-1069 -LRB- denotes (
T2677 1069-1073 NN denotes AQP3
T2678 1074-1076 CC denotes or
T2679 1077-1078 CD denotes 4
T2680 1078-1079 -RRB- denotes )
T2681 1080-1083 VBP denotes are
T2673 1084-1092 VBN denotes inserted
T2682 1093-1097 IN denotes into
T2683 1098-1101 DT denotes the
T2685 1102-1113 JJ denotes basolateral
T2684 1114-1118 NN denotes face
T2686 1118-1119 . denotes .
T2687 1119-1214 sentence denotes Unlike all other family members, AQP2 is not constitutively inserted into the plasma membrane.
T2688 1120-1126 IN denotes Unlike
T2690 1127-1130 DT denotes all
T2692 1131-1136 JJ denotes other
T2693 1137-1143 NN denotes family
T2691 1144-1151 NNS denotes members
T2694 1151-1153 , denotes ,
T2695 1153-1157 NN denotes AQP2
T2696 1158-1160 VBZ denotes is
T2697 1161-1164 RB denotes not
T2698 1165-1179 RB denotes constitutively
T2689 1180-1188 VBN denotes inserted
T2699 1189-1193 IN denotes into
T2700 1194-1197 DT denotes the
T2702 1198-1204 NN denotes plasma
T2701 1205-1213 NN denotes membrane
T2703 1213-1214 . denotes .
T2704 1214-1426 sentence denotes Under basal conditions, the protein resides in subapical intracellular vesicles; however, under conditions requiring water retention AQP2 translocates to the apical membrane, permitting water reabsorption [7,8].
T2705 1215-1220 IN denotes Under
T2707 1221-1226 JJ denotes basal
T2708 1227-1237 NNS denotes conditions
T2709 1237-1239 , denotes ,
T2710 1239-1242 DT denotes the
T2711 1243-1250 NN denotes protein
T2706 1251-1258 VBZ denotes resides
T2713 1259-1261 IN denotes in
T2714 1262-1271 JJ denotes subapical
T2716 1272-1285 JJ denotes intracellular
T2715 1286-1294 NNS denotes vesicles
T2717 1294-1295 : denotes ;
T2718 1296-1303 RB denotes however
T2719 1303-1305 , denotes ,
T2720 1305-1310 IN denotes under
T2721 1311-1321 NNS denotes conditions
T2722 1322-1331 VBG denotes requiring
T2723 1332-1337 NN denotes water
T2724 1338-1347 NN denotes retention
T2725 1348-1352 NN denotes AQP2
T2712 1353-1365 VBZ denotes translocates
T2726 1366-1368 IN denotes to
T2727 1369-1372 DT denotes the
T2729 1373-1379 JJ denotes apical
T2728 1380-1388 NN denotes membrane
T2730 1388-1390 , denotes ,
T2731 1390-1400 VBG denotes permitting
T2732 1401-1406 NN denotes water
T2733 1407-1419 NN denotes reabsorption
T2734 1420-1421 -LRB- denotes [
T2736 1421-1422 CD denotes 7
T2737 1422-1423 , denotes ,
T2735 1423-1424 CD denotes 8
T2738 1424-1425 -RRB- denotes ]
T2739 1425-1426 . denotes .
T2740 1426-1725 sentence denotes For this process to occur, AVP binds its receptor, AVPR2, on the basolateral face of the collecting duct cells, leading to a rise in intracellular cAMP, ultimately resulting in phosphorylation of AQP2 at serine 256 by cAMP-dependent protein kinase [9] and its redistribution to the plasma membrane.
T2741 1427-1430 IN denotes For
T2743 1431-1435 DT denotes this
T2744 1436-1443 NN denotes process
T2745 1444-1446 TO denotes to
T2742 1447-1452 VB denotes occur
T2747 1452-1454 , denotes ,
T2748 1454-1457 NN denotes AVP
T2746 1458-1463 VBZ denotes binds
T2749 1464-1467 PRP$ denotes its
T2750 1468-1476 NN denotes receptor
T2751 1476-1478 , denotes ,
T2752 1478-1483 NN denotes AVPR2
T2753 1483-1485 , denotes ,
T2754 1485-1487 IN denotes on
T2755 1488-1491 DT denotes the
T2757 1492-1503 JJ denotes basolateral
T2756 1504-1508 NN denotes face
T2758 1509-1511 IN denotes of
T2759 1512-1515 DT denotes the
T2761 1516-1526 VBG denotes collecting
T2762 1527-1531 NN denotes duct
T2760 1532-1537 NNS denotes cells
T2763 1537-1539 , denotes ,
T2764 1539-1546 VBG denotes leading
T2765 1547-1549 IN denotes to
T2766 1550-1551 DT denotes a
T2767 1552-1556 NN denotes rise
T2768 1557-1559 IN denotes in
T2769 1560-1573 JJ denotes intracellular
T2770 1574-1578 NN denotes cAMP
T2771 1578-1580 , denotes ,
T2772 1580-1590 RB denotes ultimately
T2773 1591-1600 VBG denotes resulting
T2774 1601-1603 IN denotes in
T2775 1604-1619 NN denotes phosphorylation
T2776 1620-1622 IN denotes of
T2777 1623-1627 NN denotes AQP2
T2778 1628-1630 IN denotes at
T2779 1631-1637 NN denotes serine
T2780 1638-1641 CD denotes 256
T2781 1642-1644 IN denotes by
T2782 1645-1649 NN denotes cAMP
T2784 1649-1650 HYPH denotes -
T2783 1650-1659 JJ denotes dependent
T2786 1660-1667 NN denotes protein
T2785 1668-1674 NN denotes kinase
T2787 1675-1676 -LRB- denotes [
T2788 1676-1677 CD denotes 9
T2789 1677-1678 -RRB- denotes ]
T2790 1679-1682 CC denotes and
T2791 1683-1686 PRP$ denotes its
T2792 1687-1701 NN denotes redistribution
T2793 1702-1704 IN denotes to
T2794 1705-1708 DT denotes the
T2796 1709-1715 NN denotes plasma
T2795 1716-1724 NN denotes membrane
T2797 1724-1725 . denotes .
T2798 1725-1875 sentence denotes The importance of AQP2 redistribution has been highlighted by functional characterization of Aqp2 mutations resulting in severe NDI in humans [3,10].
T2799 1726-1729 DT denotes The
T2800 1730-1740 NN denotes importance
T2802 1741-1743 IN denotes of
T2803 1744-1748 NN denotes AQP2
T2804 1749-1763 NN denotes redistribution
T2805 1764-1767 VBZ denotes has
T2806 1768-1772 VBN denotes been
T2801 1773-1784 VBN denotes highlighted
T2807 1785-1787 IN denotes by
T2808 1788-1798 JJ denotes functional
T2809 1799-1815 NN denotes characterization
T2810 1816-1818 IN denotes of
T2811 1819-1823 NN denotes Aqp2
T2812 1824-1833 NNS denotes mutations
T2813 1834-1843 VBG denotes resulting
T2814 1844-1846 IN denotes in
T2815 1847-1853 JJ denotes severe
T2816 1854-1857 NN denotes NDI
T2817 1858-1860 IN denotes in
T2818 1861-1867 NNS denotes humans
T2819 1868-1869 -LRB- denotes [
T2821 1869-1870 CD denotes 3
T2822 1870-1871 , denotes ,
T2820 1871-1873 CD denotes 10
T2823 1873-1874 -RRB- denotes ]
T2824 1874-1875 . denotes .
T2825 1875-2058 sentence denotes Recessive Aqp2 mutations are generally thought to produce an abnormally localized and, in most instances, misfolded water pore that responds abnormally to an increase in cAMP [6,11].
T2826 1876-1885 JJ denotes Recessive
T2828 1886-1890 NN denotes Aqp2
T2827 1891-1900 NNS denotes mutations
T2830 1901-1904 VBP denotes are
T2831 1905-1914 RB denotes generally
T2829 1915-1922 VBN denotes thought
T2832 1923-1925 TO denotes to
T2833 1926-1933 VB denotes produce
T2834 1934-1936 DT denotes an
T2836 1937-1947 RB denotes abnormally
T2837 1948-1957 VBN denotes localized
T2838 1958-1961 CC denotes and
T2839 1961-1963 , denotes ,
T2841 1963-1965 IN denotes in
T2842 1966-1970 JJS denotes most
T2843 1971-1980 NNS denotes instances
T2844 1980-1982 , denotes ,
T2840 1982-1991 VBN denotes misfolded
T2845 1992-1997 NN denotes water
T2835 1998-2002 NN denotes pore
T2846 2003-2007 WDT denotes that
T2847 2008-2016 VBZ denotes responds
T2848 2017-2027 RB denotes abnormally
T2849 2028-2030 IN denotes to
T2850 2031-2033 DT denotes an
T2851 2034-2042 NN denotes increase
T2852 2043-2045 IN denotes in
T2853 2046-2050 NN denotes cAMP
T2854 2051-2052 -LRB- denotes [
T2856 2052-2053 CD denotes 6
T2857 2053-2054 , denotes ,
T2855 2054-2056 CD denotes 11
T2858 2056-2057 -RRB- denotes ]
T2859 2057-2058 . denotes .
T2860 2058-2210 sentence denotes Furthermore, dominant mutations have been described and found to misroute both the mutant and the wild-type protein to the basolateral membrane [6,12].
T2861 2059-2070 RB denotes Furthermore
T2863 2070-2072 , denotes ,
T2864 2072-2080 JJ denotes dominant
T2865 2081-2090 NNS denotes mutations
T2866 2091-2095 VBP denotes have
T2867 2096-2100 VBN denotes been
T2862 2101-2110 VBN denotes described
T2868 2111-2114 CC denotes and
T2869 2115-2120 VBN denotes found
T2870 2121-2123 TO denotes to
T2871 2124-2132 VB denotes misroute
T2872 2133-2137 CC denotes both
T2874 2138-2141 DT denotes the
T2873 2142-2148 NN denotes mutant
T2875 2149-2152 CC denotes and
T2876 2153-2156 DT denotes the
T2878 2157-2161 JJ denotes wild
T2879 2161-2162 HYPH denotes -
T2877 2162-2166 NN denotes type
T2880 2167-2174 NN denotes protein
T2881 2175-2177 IN denotes to
T2882 2178-2181 DT denotes the
T2884 2182-2193 JJ denotes basolateral
T2883 2194-2202 NN denotes membrane
T2885 2203-2204 -LRB- denotes [
T2887 2204-2205 CD denotes 6
T2888 2205-2206 , denotes ,
T2886 2206-2208 CD denotes 12
T2889 2208-2209 -RRB- denotes ]
T2890 2209-2210 . denotes .
T2891 2210-2282 sentence denotes Several mouse models of diabetes insipidus have been generated [13–17].
T2892 2211-2218 JJ denotes Several
T2894 2219-2224 NN denotes mouse
T2893 2225-2231 NNS denotes models
T2896 2232-2234 IN denotes of
T2897 2235-2243 NN denotes diabetes
T2898 2244-2253 NN denotes insipidus
T2899 2254-2258 VBP denotes have
T2900 2259-2263 VBN denotes been
T2895 2264-2273 VBN denotes generated
T2901 2274-2275 -LRB- denotes [
T2902 2275-2277 CD denotes 13
T2903 2277-2278 SYM denotes –
T2904 2278-2280 CD denotes 17
T2905 2280-2281 -RRB- denotes ]
T2906 2281-2282 . denotes .
T2907 2282-2390 sentence denotes In an attempt to recapitulate human NDI, mice have been generated with mutations in Aqp2 and Avpr2 [15,18].
T2908 2283-2285 IN denotes In
T2910 2286-2288 DT denotes an
T2911 2289-2296 NN denotes attempt
T2912 2297-2299 TO denotes to
T2913 2300-2312 VB denotes recapitulate
T2914 2313-2318 JJ denotes human
T2915 2319-2322 NN denotes NDI
T2916 2322-2324 , denotes ,
T2917 2324-2328 NNS denotes mice
T2918 2329-2333 VBP denotes have
T2919 2334-2338 VBN denotes been
T2909 2339-2348 VBN denotes generated
T2920 2349-2353 IN denotes with
T2921 2354-2363 NNS denotes mutations
T2922 2364-2366 IN denotes in
T2923 2367-2371 NN denotes Aqp2
T2924 2372-2375 CC denotes and
T2925 2376-2381 NN denotes Avpr2
T2926 2382-2383 -LRB- denotes [
T2928 2383-2385 CD denotes 15
T2929 2385-2386 , denotes ,
T2927 2386-2388 CD denotes 18
T2930 2388-2389 -RRB- denotes ]
T2931 2389-2390 . denotes .
T2932 2390-2475 sentence denotes Yang and colleagues created a mouse with a T126M knock-in mutation in the Aqp2 gene.
T2933 2391-2395 NNP denotes Yang
T2935 2396-2399 CC denotes and
T2936 2400-2410 NNS denotes colleagues
T2934 2411-2418 VBD denotes created
T2937 2419-2420 DT denotes a
T2938 2421-2426 NN denotes mouse
T2939 2427-2431 IN denotes with
T2940 2432-2433 DT denotes a
T2942 2434-2439 NN denotes T126M
T2943 2440-2445 VB denotes knock
T2944 2445-2446 HYPH denotes -
T2945 2446-2448 RP denotes in
T2941 2449-2457 NN denotes mutation
T2946 2458-2460 IN denotes in
T2947 2461-2464 DT denotes the
T2949 2465-2469 NN denotes Aqp2
T2948 2470-2474 NN denotes gene
T2950 2474-2475 . denotes .
T2951 2475-2541 sentence denotes Unexpectedly, homozygous mutant mice died within 6 d after birth.
T2952 2476-2488 RB denotes Unexpectedly
T2954 2488-2490 , denotes ,
T2955 2490-2500 JJ denotes homozygous
T2957 2501-2507 NN denotes mutant
T2956 2508-2512 NNS denotes mice
T2953 2513-2517 VBD denotes died
T2958 2518-2524 IN denotes within
T2959 2525-2526 CD denotes 6
T2960 2527-2528 NNS denotes d
T2961 2529-2534 IN denotes after
T2962 2535-2540 NN denotes birth
T2963 2540-2541 . denotes .
T2964 2541-2626 sentence denotes Interestingly, AVPR2-deficient male pups also die within the first week after birth.
T2965 2542-2555 RB denotes Interestingly
T2967 2555-2557 , denotes ,
T2968 2557-2562 NN denotes AVPR2
T2970 2562-2563 HYPH denotes -
T2969 2563-2572 JJ denotes deficient
T2972 2573-2577 JJ denotes male
T2971 2578-2582 NNS denotes pups
T2973 2583-2587 RB denotes also
T2966 2588-2591 VBP denotes die
T2974 2592-2598 IN denotes within
T2975 2599-2602 DT denotes the
T2977 2603-2608 JJ denotes first
T2976 2609-2613 NN denotes week
T2978 2614-2619 IN denotes after
T2979 2620-2625 NN denotes birth
T2980 2625-2626 . denotes .
T2981 2626-2780 sentence denotes Together these models suggest that the mouse may be a highly sensitive organism with regard to water homeostasis, and is unable to survive with polyuria.
T2982 2627-2635 RB denotes Together
T2984 2636-2641 DT denotes these
T2985 2642-2648 NNS denotes models
T2983 2649-2656 VBP denotes suggest
T2986 2657-2661 IN denotes that
T2988 2662-2665 DT denotes the
T2989 2666-2671 NN denotes mouse
T2990 2672-2675 MD denotes may
T2987 2676-2678 VB denotes be
T2991 2679-2680 DT denotes a
T2993 2681-2687 RB denotes highly
T2994 2688-2697 JJ denotes sensitive
T2992 2698-2706 NN denotes organism
T2995 2707-2711 IN denotes with
T2996 2712-2718 NN denotes regard
T2997 2719-2721 IN denotes to
T2998 2722-2727 NN denotes water
T2999 2728-2739 NN denotes homeostasis
T3000 2739-2741 , denotes ,
T3001 2741-2744 CC denotes and
T3002 2745-2747 VBZ denotes is
T3003 2748-2754 JJ denotes unable
T3004 2755-2757 TO denotes to
T3005 2758-2765 VB denotes survive
T3006 2766-2770 IN denotes with
T3007 2771-2779 NN denotes polyuria
T3008 2779-2780 . denotes .
T3009 2780-2855 sentence denotes In a forward genetic screen, a mouse with an Aqp2 mutation was identified.
T3010 2781-2783 IN denotes In
T3012 2784-2785 DT denotes a
T3014 2786-2793 RB denotes forward
T3015 2794-2801 JJ denotes genetic
T3013 2802-2808 NN denotes screen
T3016 2808-2810 , denotes ,
T3017 2810-2811 DT denotes a
T3018 2812-2817 NN denotes mouse
T3019 2818-2822 IN denotes with
T3020 2823-2825 DT denotes an
T3022 2826-2830 NN denotes Aqp2
T3021 2831-2839 NN denotes mutation
T3023 2840-2843 VBD denotes was
T3011 2844-2854 VBN denotes identified
T3024 2854-2855 . denotes .
T3025 2855-2948 sentence denotes The purpose of this study was to characterize this murine model of recessive nephrogenic DI.
T3026 2856-2859 DT denotes The
T3027 2860-2867 NN denotes purpose
T3029 2868-2870 IN denotes of
T3030 2871-2875 DT denotes this
T3031 2876-2881 NN denotes study
T3028 2882-2885 VBD denotes was
T3032 2886-2888 TO denotes to
T3033 2889-2901 VB denotes characterize
T3034 2902-2906 DT denotes this
T3036 2907-2913 JJ denotes murine
T3035 2914-2919 NN denotes model
T3037 2920-2922 IN denotes of
T3038 2923-2932 JJ denotes recessive
T3040 2933-2944 JJ denotes nephrogenic
T3039 2945-2947 NN denotes DI
T3041 2947-2948 . denotes .
T3042 2948-3003 sentence denotes We now report a novel F204V mutation in the Aqp2 gene.
T3043 2949-2951 PRP denotes We
T3045 2952-2955 RB denotes now
T3044 2956-2962 VBP denotes report
T3046 2963-2964 DT denotes a
T3048 2965-2970 JJ denotes novel
T3049 2971-2976 NN denotes F204V
T3047 2977-2985 NN denotes mutation
T3050 2986-2988 IN denotes in
T3051 2989-2992 DT denotes the
T3053 2993-2997 NN denotes Aqp2
T3052 2998-3002 NN denotes gene
T3054 3002-3003 . denotes .
T3055 3003-3111 sentence denotes This allele of Aqp2 was found to cause the first mouse model of NDI to survive past the first week of life.
T3056 3004-3008 DT denotes This
T3057 3009-3015 NN denotes allele
T3059 3016-3018 IN denotes of
T3060 3019-3023 NN denotes Aqp2
T3061 3024-3027 VBD denotes was
T3058 3028-3033 VBN denotes found
T3062 3034-3036 TO denotes to
T3063 3037-3042 VB denotes cause
T3064 3043-3046 DT denotes the
T3066 3047-3052 JJ denotes first
T3067 3053-3058 NN denotes mouse
T3065 3059-3064 NN denotes model
T3069 3065-3067 IN denotes of
T3070 3068-3071 NN denotes NDI
T3071 3072-3074 TO denotes to
T3068 3075-3082 VB denotes survive
T3072 3083-3087 IN denotes past
T3073 3088-3091 DT denotes the
T3075 3092-3097 JJ denotes first
T3074 3098-3102 NN denotes week
T3076 3103-3105 IN denotes of
T3077 3106-3110 NN denotes life
T3078 3110-3111 . denotes .
T3079 3111-3312 sentence denotes Molecular analyses concluded that mutant AQP2 adopts a different subcellular localization in renal collecting-duct cells, and was resistant to translocation induced by desmopressin, an agonist of AVP.
T3080 3112-3121 JJ denotes Molecular
T3081 3122-3130 NNS denotes analyses
T3082 3131-3140 VBD denotes concluded
T3083 3141-3145 IN denotes that
T3085 3146-3152 NN denotes mutant
T3086 3153-3157 NN denotes AQP2
T3084 3158-3164 VBZ denotes adopts
T3087 3165-3166 DT denotes a
T3089 3167-3176 JJ denotes different
T3090 3177-3188 JJ denotes subcellular
T3088 3189-3201 NN denotes localization
T3091 3202-3204 IN denotes in
T3092 3205-3210 JJ denotes renal
T3094 3211-3221 VBG denotes collecting
T3096 3221-3222 HYPH denotes -
T3095 3222-3226 NN denotes duct
T3093 3227-3232 NNS denotes cells
T3097 3232-3234 , denotes ,
T3098 3234-3237 CC denotes and
T3099 3238-3241 VBD denotes was
T3100 3242-3251 JJ denotes resistant
T3101 3252-3254 IN denotes to
T3102 3255-3268 NN denotes translocation
T3103 3269-3276 VBN denotes induced
T3104 3277-3279 IN denotes by
T3105 3280-3292 NN denotes desmopressin
T3106 3292-3294 , denotes ,
T3107 3294-3296 DT denotes an
T3108 3297-3304 NN denotes agonist
T3109 3305-3307 IN denotes of
T3110 3308-3311 NN denotes AVP
T3111 3311-3312 . denotes .
T3112 3312-3494 sentence denotes In vitro studies using the Madin-Darby canine kidney (MDCK) cell line demonstrated an endoplasmic reticulum pattern for the mutant protein, and apparent resistance to translocation.
T3113 3313-3315 FW denotes In
T3114 3316-3321 FW denotes vitro
T3115 3322-3329 NNS denotes studies
T3117 3330-3335 VBG denotes using
T3118 3336-3339 DT denotes the
T3120 3340-3345 NN denotes Madin
T3122 3345-3346 HYPH denotes -
T3121 3346-3351 NN denotes Darby
T3124 3352-3358 JJ denotes canine
T3123 3359-3365 NN denotes kidney
T3125 3366-3367 -LRB- denotes (
T3126 3367-3371 NN denotes MDCK
T3127 3371-3372 -RRB- denotes )
T3128 3373-3377 NN denotes cell
T3119 3378-3382 NN denotes line
T3116 3383-3395 VBD denotes demonstrated
T3129 3396-3398 DT denotes an
T3131 3399-3410 JJ denotes endoplasmic
T3132 3411-3420 NN denotes reticulum
T3130 3421-3428 NN denotes pattern
T3133 3429-3432 IN denotes for
T3134 3433-3436 DT denotes the
T3136 3437-3443 NN denotes mutant
T3135 3444-3451 NN denotes protein
T3137 3451-3453 , denotes ,
T3138 3453-3456 CC denotes and
T3139 3457-3465 JJ denotes apparent
T3140 3466-3476 NN denotes resistance
T3141 3477-3479 IN denotes to
T3142 3480-3493 NN denotes translocation
T3143 3493-3494 . denotes .
T3144 3494-3617 sentence denotes These data conclusively prove that autosomal recessive NDI is a consequence of improper AQP2 routing in the intact mammal.
T3145 3495-3500 DT denotes These
T3146 3501-3505 NNS denotes data
T3148 3506-3518 RB denotes conclusively
T3147 3519-3524 VBP denotes prove
T3149 3525-3529 IN denotes that
T3151 3530-3539 JJ denotes autosomal
T3153 3540-3549 JJ denotes recessive
T3152 3550-3553 NN denotes NDI
T3150 3554-3556 VBZ denotes is
T3154 3557-3558 DT denotes a
T3155 3559-3570 NN denotes consequence
T3156 3571-3573 IN denotes of
T3157 3574-3582 JJ denotes improper
T3159 3583-3587 NN denotes AQP2
T3158 3588-3595 NN denotes routing
T3160 3596-3598 IN denotes in
T3161 3599-3602 DT denotes the
T3163 3603-3609 JJ denotes intact
T3162 3610-3616 NN denotes mammal
T3164 3616-3617 . denotes .
T8951 3627-3629 IN denotes In
T8953 3630-3631 DT denotes a
T8955 3632-3639 JJ denotes forward
T8956 3640-3647 JJ denotes genetic
T8954 3648-3654 NN denotes screen
T8957 3655-3659 WDT denotes that
T8958 3660-3664 VBD denotes used
T8959 3665-3681 NN denotes ethylnitrosourea
T8960 3682-3683 -LRB- denotes (
T8961 3683-3686 NN denotes ENU
T8962 3686-3687 -RRB- denotes )
T8963 3688-3690 TO denotes to
T8964 3691-3697 VB denotes induce
T8965 3698-3707 NNS denotes mutations
T8966 3708-3710 IN denotes in
T8967 3711-3712 DT denotes a
T8969 3713-3720 NN denotes founder
T8968 3721-3727 NN denotes animal
T8970 3728-3733 WP$ denotes whose
T8971 3734-3743 NN denotes offspring
T8973 3744-3748 VBD denotes were
T8974 3749-3753 RB denotes then
T8972 3754-3762 VBN denotes screened
T8975 3763-3766 IN denotes for
T8976 3767-3775 JJ denotes abnormal
T8978 3776-3781 JJ denotes whole
T8979 3782-3786 NN denotes body
T8977 3787-3797 NN denotes metabolism
T8980 3798-3799 -LRB- denotes [
T8982 3799-3801 CD denotes 19
T8983 3801-3802 , denotes ,
T8981 3802-3804 CD denotes 20
T8984 3804-3805 -RRB- denotes ]
T8985 3805-3807 , denotes ,
T8986 3807-3809 PRP denotes we
T8952 3810-3815 VBD denotes found
T8987 3816-3817 DT denotes a
T8988 3818-3824 NN denotes family
T8989 3825-3827 IN denotes of
T8990 3828-3832 NNS denotes mice
T8991 3833-3837 WDT denotes that
T8992 3838-3846 VBD denotes urinated
T8993 3847-3850 CC denotes and
T8994 3851-3856 VBD denotes drank
T8995 3857-3868 RB denotes excessively
T8996 3868-3869 . denotes .
T8997 3869-3998 sentence denotes Serum and urine analysis showed that plasma glucose levels were normal and there was no glucose in the urine (unpublished data).
T8998 3870-3875 NN denotes Serum
T9000 3876-3879 CC denotes and
T9001 3880-3885 NN denotes urine
T8999 3886-3894 NN denotes analysis
T9002 3895-3901 VBD denotes showed
T9003 3902-3906 IN denotes that
T9005 3907-3913 NN denotes plasma
T9007 3914-3921 NN denotes glucose
T9006 3922-3928 NNS denotes levels
T9004 3929-3933 VBD denotes were
T9008 3934-3940 JJ denotes normal
T9009 3941-3944 CC denotes and
T9010 3945-3950 EX denotes there
T9011 3951-3954 VBD denotes was
T9012 3955-3957 DT denotes no
T9013 3958-3965 NN denotes glucose
T9014 3966-3968 IN denotes in
T9015 3969-3972 DT denotes the
T9016 3973-3978 NN denotes urine
T9017 3979-3980 -LRB- denotes (
T9019 3980-3991 JJ denotes unpublished
T9018 3992-3996 NNS denotes data
T9020 3996-3997 -RRB- denotes )
T9021 3997-3998 . denotes .
T9022 3998-4048 sentence denotes Hence, this was an example of diabetes insipidus.
T9023 3999-4004 RB denotes Hence
T9025 4004-4006 , denotes ,
T9026 4006-4010 DT denotes this
T9024 4011-4014 VBD denotes was
T9027 4015-4017 DT denotes an
T9028 4018-4025 NN denotes example
T9029 4026-4028 IN denotes of
T9030 4029-4037 NN denotes diabetes
T9031 4038-4047 NN denotes insipidus
T9032 4047-4048 . denotes .
T9033 4048-4160 sentence denotes The disorder in these mice segregated in a monogenic, autosomal recessive manner, making Aqp2 a candidate gene.
T9034 4049-4052 DT denotes The
T9035 4053-4061 NN denotes disorder
T9037 4062-4064 IN denotes in
T9038 4065-4070 DT denotes these
T9039 4071-4075 NNS denotes mice
T9036 4076-4086 VBD denotes segregated
T9040 4087-4089 IN denotes in
T9041 4090-4091 DT denotes a
T9043 4092-4101 JJ denotes monogenic
T9044 4101-4103 , denotes ,
T9045 4103-4112 JJ denotes autosomal
T9046 4113-4122 JJ denotes recessive
T9042 4123-4129 NN denotes manner
T9047 4129-4131 , denotes ,
T9048 4131-4137 VBG denotes making
T9049 4138-4142 NN denotes Aqp2
T9051 4143-4144 DT denotes a
T9052 4145-4154 NN denotes candidate
T9050 4155-4159 NN denotes gene
T9053 4159-4160 . denotes .
T9054 4160-4389 sentence denotes Sequencing of Aqp2 coding region of affected mice identified a thymine to guanine (T to G) transversion (Figure 1A), which is predicted to lead to a valine for phenylalanine substitution at amino acid 204 of the protein (F204V).
T9055 4161-4171 NN denotes Sequencing
T9057 4172-4174 IN denotes of
T9058 4175-4179 NN denotes Aqp2
T9060 4180-4186 NN denotes coding
T9059 4187-4193 NN denotes region
T9061 4194-4196 IN denotes of
T9062 4197-4205 VBN denotes affected
T9063 4206-4210 NNS denotes mice
T9056 4211-4221 VBD denotes identified
T9064 4222-4223 DT denotes a
T9066 4224-4231 NN denotes thymine
T9067 4232-4234 IN denotes to
T9068 4235-4242 NN denotes guanine
T9069 4243-4244 -LRB- denotes (
T9070 4244-4245 NN denotes T
T9071 4246-4248 IN denotes to
T9072 4249-4250 NN denotes G
T9073 4250-4251 -RRB- denotes )
T9065 4252-4264 NN denotes transversion
T9074 4265-4266 -LRB- denotes (
T9075 4266-4272 NN denotes Figure
T9076 4273-4275 CD denotes 1A
T9077 4275-4276 -RRB- denotes )
T9078 4276-4278 , denotes ,
T9079 4278-4283 WDT denotes which
T9081 4284-4286 VBZ denotes is
T9080 4287-4296 VBN denotes predicted
T9082 4297-4299 TO denotes to
T9083 4300-4304 VB denotes lead
T9084 4305-4307 IN denotes to
T9085 4308-4309 DT denotes a
T9087 4310-4316 NN denotes valine
T9088 4317-4320 IN denotes for
T9089 4321-4334 NN denotes phenylalanine
T9086 4335-4347 NN denotes substitution
T9090 4348-4350 IN denotes at
T9091 4351-4356 NN denotes amino
T9092 4357-4361 NN denotes acid
T9093 4362-4365 CD denotes 204
T9094 4366-4368 IN denotes of
T9095 4369-4372 DT denotes the
T9096 4373-4380 NN denotes protein
T9097 4381-4382 -LRB- denotes (
T9098 4382-4387 NN denotes F204V
T9099 4387-4388 -RRB- denotes )
T9100 4388-4389 . denotes .
T9101 4389-5579 sentence denotes Figure 1 Analysis of Aqp2 Sequence and Phenotype in Mutant Mice (A) Chromatographic traces of Aqp2 F204V mutation. The box shows the mutated codon, TTC (Phe) to GTC (Val) at position 204. WT, wild type; Mut, mutant. (B) Amino acid conservation of mouse AQP2 (residues 194–214). The boxed residue indicates phenylalanine at position 204. hAQP2, human AQP2; mAQP1, mouse AQP1; mAQP2, mouse AQP2; rAQP2, rat AQP2; xAQP2, Xenopus AQP2. (C) Urine production (ml) and water consumption (ml) of 58 F2 mice over a 24-h period (both sexes, aged 10–22 wk). Mutant mice (black squares) exhibit overt polyuria and polydipsia compared to littermate wild-type (white triangles) and heterozygous (grey circles) mice. (D) Urine osmolality and concentrating ability in Aqp2 mutant and their littermates (10–22 wk, both sexes), before (white bars) and after (black bars) dDAVP treatment. Wild type (WT; n = 12); heterozygote (Het; n = 20); mutant (Mut; n = 9). Data represent averages ± standard error of the mean, **p < 0.01; ***p < 0.001. AQP2 is a six-transmembrane water channel, and F204 lies near the extracellular face of the sixth membrane spanning domain, a region rich in hydrophobic amino acids.
T9102 5414-5418 NN denotes AQP2
T9103 5419-5421 VBZ denotes is
T9104 5422-5423 DT denotes a
T9106 5424-5427 CD denotes six
T9108 5427-5428 HYPH denotes -
T9107 5428-5441 NN denotes transmembrane
T9109 5442-5447 NN denotes water
T9105 5448-5455 NN denotes channel
T9110 5455-5457 , denotes ,
T9111 5457-5460 CC denotes and
T9112 5461-5465 NN denotes F204
T9113 5466-5470 VBZ denotes lies
T9114 5471-5475 IN denotes near
T9115 5476-5479 DT denotes the
T9117 5480-5493 JJ denotes extracellular
T9116 5494-5498 NN denotes face
T9118 5499-5501 IN denotes of
T9119 5502-5505 DT denotes the
T9121 5506-5511 JJ denotes sixth
T9122 5512-5520 NN denotes membrane
T9123 5521-5529 VBG denotes spanning
T9120 5530-5536 NN denotes domain
T9124 5536-5538 , denotes ,
T9125 5538-5539 DT denotes a
T9126 5540-5546 NN denotes region
T9127 5547-5551 JJ denotes rich
T9128 5552-5554 IN denotes in
T9129 5555-5566 JJ denotes hydrophobic
T9131 5567-5572 NN denotes amino
T9130 5573-5578 NNS denotes acids
T9132 5578-5579 . denotes .
T9133 5579-5664 sentence denotes This and the other membrane-spanning domains are conserved among vertebrate species.
T9134 5580-5584 DT denotes This
T9136 5585-5588 CC denotes and
T9137 5589-5592 DT denotes the
T9138 5593-5598 JJ denotes other
T9140 5599-5607 NN denotes membrane
T9142 5607-5608 HYPH denotes -
T9141 5608-5616 VBG denotes spanning
T9139 5617-5624 NNS denotes domains
T9143 5625-5628 VBP denotes are
T9135 5629-5638 VBN denotes conserved
T9144 5639-5644 IN denotes among
T9145 5645-5655 JJ denotes vertebrate
T9146 5656-5663 NNS denotes species
T9147 5663-5664 . denotes .
T9148 5664-5829 sentence denotes The phenylalanine at position 204 is particularly well conserved (Figure 1B), not only among vertebrate AQP2 proteins, but also among others members of this family.
T9149 5665-5668 DT denotes The
T9150 5669-5682 NN denotes phenylalanine
T9152 5683-5685 IN denotes at
T9153 5686-5694 NN denotes position
T9154 5695-5698 CD denotes 204
T9151 5699-5701 VBZ denotes is
T9155 5702-5714 RB denotes particularly
T9157 5715-5719 RB denotes well
T9156 5720-5729 VBN denotes conserved
T9158 5730-5731 -LRB- denotes (
T9159 5731-5737 NN denotes Figure
T9160 5738-5740 CD denotes 1B
T9161 5740-5741 -RRB- denotes )
T9162 5741-5743 , denotes ,
T9163 5743-5746 RB denotes not
T9165 5747-5751 RB denotes only
T9164 5752-5757 IN denotes among
T9166 5758-5768 JJ denotes vertebrate
T9168 5769-5773 NN denotes AQP2
T9167 5774-5782 NN denotes proteins
T9169 5782-5784 , denotes ,
T9170 5784-5787 CC denotes but
T9171 5788-5792 RB denotes also
T9172 5793-5798 IN denotes among
T9173 5799-5805 NNS denotes others
T9174 5806-5813 NNS denotes members
T9175 5814-5816 IN denotes of
T9176 5817-5821 DT denotes this
T9177 5822-5828 NN denotes family
T9178 5828-5829 . denotes .
T9179 5829-6036 sentence denotes Aqp2F204V/F204V mice have dramatically increased urine production, in some cases producing an amount of urine in 24 h that exceeds their body weight, compared to their heterozygous or wild-type littermates.
T9180 5830-5839 NN denotes Aqp2F204V
T9182 5839-5840 HYPH denotes /
T9181 5840-5845 NN denotes F204V
T9183 5846-5850 NNS denotes mice
T9184 5851-5855 VBP denotes have
T9185 5856-5868 RB denotes dramatically
T9186 5869-5878 VBN denotes increased
T9188 5879-5884 NN denotes urine
T9187 5885-5895 NN denotes production
T9189 5895-5897 , denotes ,
T9190 5897-5899 IN denotes in
T9192 5900-5904 DT denotes some
T9193 5905-5910 NNS denotes cases
T9191 5911-5920 VBG denotes producing
T9194 5921-5923 DT denotes an
T9195 5924-5930 NN denotes amount
T9196 5931-5933 IN denotes of
T9197 5934-5939 NN denotes urine
T9198 5940-5942 IN denotes in
T9199 5943-5945 CD denotes 24
T9200 5946-5947 NN denotes h
T9201 5948-5952 WDT denotes that
T9202 5953-5960 VBZ denotes exceeds
T9203 5961-5966 PRP$ denotes their
T9205 5967-5971 NN denotes body
T9204 5972-5978 NN denotes weight
T9206 5978-5980 , denotes ,
T9207 5980-5988 VBN denotes compared
T9208 5989-5991 IN denotes to
T9209 5992-5997 PRP$ denotes their
T9211 5998-6010 JJ denotes heterozygous
T9212 6011-6013 CC denotes or
T9213 6014-6018 JJ denotes wild
T9215 6018-6019 HYPH denotes -
T9214 6019-6023 NN denotes type
T9210 6024-6035 NNS denotes littermates
T9216 6035-6036 . denotes .
T9217 6036-6140 sentence denotes Such loss of water would rapidly lead to dehydration were it not compensated by increased water intake.
T9218 6037-6041 JJ denotes Such
T9219 6042-6046 NN denotes loss
T9221 6047-6049 IN denotes of
T9222 6050-6055 NN denotes water
T9223 6056-6061 MD denotes would
T9224 6062-6069 RB denotes rapidly
T9220 6070-6074 VB denotes lead
T9225 6075-6077 IN denotes to
T9226 6078-6089 NN denotes dehydration
T9227 6090-6094 VBD denotes were
T9229 6095-6097 PRP denotes it
T9230 6098-6101 RB denotes not
T9228 6102-6113 VBN denotes compensated
T9231 6114-6116 IN denotes by
T9232 6117-6126 VBN denotes increased
T9234 6127-6132 NN denotes water
T9233 6133-6139 NN denotes intake
T9235 6139-6140 . denotes .
T9236 6140-6275 sentence denotes Indeed, mutant mice also dramatically increase their water intake (Figure 1C) compared to their heterozygous or wild-type littermates.
T9237 6141-6147 RB denotes Indeed
T9239 6147-6149 , denotes ,
T9240 6149-6155 NN denotes mutant
T9241 6156-6160 NNS denotes mice
T9242 6161-6165 RB denotes also
T9243 6166-6178 RB denotes dramatically
T9238 6179-6187 VB denotes increase
T9244 6188-6193 PRP$ denotes their
T9246 6194-6199 NN denotes water
T9245 6200-6206 NN denotes intake
T9247 6207-6208 -LRB- denotes (
T9248 6208-6214 NN denotes Figure
T9249 6215-6217 CD denotes 1C
T9250 6217-6218 -RRB- denotes )
T9251 6219-6227 VBN denotes compared
T9252 6228-6230 IN denotes to
T9253 6231-6236 PRP$ denotes their
T9255 6237-6249 JJ denotes heterozygous
T9256 6250-6252 CC denotes or
T9257 6253-6257 JJ denotes wild
T9259 6257-6258 HYPH denotes -
T9258 6258-6262 NN denotes type
T9254 6263-6274 NNS denotes littermates
T9260 6274-6275 . denotes .
T9261 6275-6426 sentence denotes This phenotype—increased urinary output and water intake—showed complete concordance with homozygosity of the F204V mutation in the 58 animals tested.
T9262 6276-6280 DT denotes This
T9263 6281-6290 NN denotes phenotype
T9265 6290-6291 , denotes —
T9266 6291-6300 VBN denotes increased
T9268 6301-6308 JJ denotes urinary
T9267 6309-6315 NN denotes output
T9269 6316-6319 CC denotes and
T9270 6320-6325 NN denotes water
T9271 6326-6332 NN denotes intake
T9272 6332-6333 , denotes —
T9264 6333-6339 VBD denotes showed
T9273 6340-6348 JJ denotes complete
T9274 6349-6360 NN denotes concordance
T9275 6361-6365 IN denotes with
T9276 6366-6378 NN denotes homozygosity
T9277 6379-6381 IN denotes of
T9278 6382-6385 DT denotes the
T9280 6386-6391 NN denotes F204V
T9279 6392-6400 NN denotes mutation
T9281 6401-6403 IN denotes in
T9282 6404-6407 DT denotes the
T9284 6408-6410 CD denotes 58
T9283 6411-6418 NNS denotes animals
T9285 6419-6425 VBN denotes tested
T9286 6425-6426 . denotes .
T9287 6426-6516 sentence denotes Diabetes insipidus can be defined as an inability to concentrate urine where appropriate.
T9288 6427-6435 NN denotes Diabetes
T9289 6436-6445 NN denotes insipidus
T9291 6446-6449 MD denotes can
T9292 6450-6452 VB denotes be
T9290 6453-6460 VBN denotes defined
T9293 6461-6463 IN denotes as
T9294 6464-6466 DT denotes an
T9295 6467-6476 NN denotes inability
T9296 6477-6479 TO denotes to
T9297 6480-6491 VB denotes concentrate
T9298 6492-6497 NN denotes urine
T9299 6498-6503 WRB denotes where
T9300 6504-6515 JJ denotes appropriate
T9301 6515-6516 . denotes .
T9302 6516-6627 sentence denotes Compared to wild-type or heterozygous littermates, Aqp2F204V/F204V mice produce very dilute urine (Figure 1D).
T9303 6517-6525 VBN denotes Compared
T9305 6526-6528 IN denotes to
T9306 6529-6533 JJ denotes wild
T9308 6533-6534 HYPH denotes -
T9307 6534-6538 NN denotes type
T9310 6539-6541 CC denotes or
T9311 6542-6554 JJ denotes heterozygous
T9309 6555-6566 NNS denotes littermates
T9312 6566-6568 , denotes ,
T9313 6568-6577 NN denotes Aqp2F204V
T9315 6577-6578 HYPH denotes /
T9314 6578-6583 NN denotes F204V
T9316 6584-6588 NNS denotes mice
T9304 6589-6596 VBP denotes produce
T9317 6597-6601 RB denotes very
T9318 6602-6608 JJ denotes dilute
T9319 6609-6614 NN denotes urine
T9320 6615-6616 -LRB- denotes (
T9321 6616-6622 NN denotes Figure
T9322 6623-6625 CD denotes 1D
T9323 6625-6626 -RRB- denotes )
T9324 6626-6627 . denotes .
T9325 6627-6747 sentence denotes Basal urine concentration in mutant mice is about 161 mOsm, compared to about 1,293 mOsm in wild-type mice (p < 0.001).
T9326 6628-6633 JJ denotes Basal
T9328 6634-6639 NN denotes urine
T9327 6640-6653 NN denotes concentration
T9330 6654-6656 IN denotes in
T9331 6657-6663 NN denotes mutant
T9332 6664-6668 NNS denotes mice
T9329 6669-6671 VBZ denotes is
T9333 6672-6677 RB denotes about
T9334 6678-6681 CD denotes 161
T9335 6682-6686 NN denotes mOsm
T9336 6686-6688 , denotes ,
T9337 6688-6696 VBN denotes compared
T9338 6697-6699 IN denotes to
T9339 6700-6705 IN denotes about
T9340 6706-6711 CD denotes 1,293
T9341 6712-6716 NN denotes mOsm
T9342 6717-6719 IN denotes in
T9343 6720-6724 JJ denotes wild
T9345 6724-6725 HYPH denotes -
T9344 6725-6729 NN denotes type
T9346 6730-6734 NNS denotes mice
T9347 6735-6736 -LRB- denotes (
T9349 6736-6737 NN denotes p
T9350 6738-6739 SYM denotes <
T9348 6740-6745 CD denotes 0.001
T9351 6745-6746 -RRB- denotes )
T9352 6746-6747 . denotes .
T9353 6747-6890 sentence denotes Normally, urine concentration is under the control of the hypothalamus, which, in response to hypovolemia or hypernatremia [21], secretes AVP.
T9354 6748-6756 RB denotes Normally
T9356 6756-6758 , denotes ,
T9357 6758-6763 NN denotes urine
T9358 6764-6777 NN denotes concentration
T9355 6778-6780 VBZ denotes is
T9359 6781-6786 IN denotes under
T9360 6787-6790 DT denotes the
T9361 6791-6798 NN denotes control
T9362 6799-6801 IN denotes of
T9363 6802-6805 DT denotes the
T9364 6806-6818 NN denotes hypothalamus
T9365 6818-6820 , denotes ,
T9366 6820-6825 WDT denotes which
T9368 6825-6827 , denotes ,
T9369 6827-6829 IN denotes in
T9370 6830-6838 NN denotes response
T9371 6839-6841 IN denotes to
T9372 6842-6853 NN denotes hypovolemia
T9373 6854-6856 CC denotes or
T9374 6857-6870 NN denotes hypernatremia
T9375 6871-6872 -LRB- denotes [
T9376 6872-6874 CD denotes 21
T9377 6874-6875 -RRB- denotes ]
T9378 6875-6877 , denotes ,
T9367 6877-6885 VBZ denotes secretes
T9379 6886-6889 NN denotes AVP
T9380 6889-6890 . denotes .
T9381 6890-7016 sentence denotes The synthetic AVP analog, 1-deamino-8-D-arginine vasopressin (dDAVP; also called desmopressin), is a potent agonist of AVPR2.
T9382 6891-6894 DT denotes The
T9384 6895-6904 JJ denotes synthetic
T9385 6905-6908 NN denotes AVP
T9383 6909-6915 NN denotes analog
T9387 6915-6917 , denotes ,
T9388 6917-6918 CD denotes 1
T9390 6918-6919 HYPH denotes -
T9389 6919-6926 NN denotes deamino
T9392 6926-6927 HYPH denotes -
T9393 6927-6928 CD denotes 8
T9394 6928-6929 HYPH denotes -
T9395 6929-6930 NN denotes D
T9396 6930-6931 HYPH denotes -
T9391 6931-6939 NN denotes arginine
T9397 6940-6951 NN denotes vasopressin
T9398 6952-6953 -LRB- denotes (
T9399 6953-6958 NN denotes dDAVP
T9400 6958-6959 : denotes ;
T9401 6960-6964 RB denotes also
T9402 6965-6971 VBN denotes called
T9403 6972-6984 NN denotes desmopressin
T9404 6984-6985 -RRB- denotes )
T9405 6985-6987 , denotes ,
T9386 6987-6989 VBZ denotes is
T9406 6990-6991 DT denotes a
T9408 6992-6998 JJ denotes potent
T9407 6999-7006 NN denotes agonist
T9409 7007-7009 IN denotes of
T9410 7010-7015 NN denotes AVPR2
T9411 7015-7016 . denotes .
T9412 7016-7160 sentence denotes When administered to wild-type mice, dDAVP leads to a dramatic increase in urine concentration, from 1,293 to 5,885 mOsm (4.6-fold; Figure 1D).
T9413 7017-7021 WRB denotes When
T9414 7022-7034 VBN denotes administered
T9416 7035-7037 IN denotes to
T9417 7038-7042 JJ denotes wild
T9419 7042-7043 HYPH denotes -
T9418 7043-7047 NN denotes type
T9420 7048-7052 NNS denotes mice
T9421 7052-7054 , denotes ,
T9422 7054-7059 NN denotes dDAVP
T9415 7060-7065 VBZ denotes leads
T9423 7066-7068 IN denotes to
T9424 7069-7070 DT denotes a
T9426 7071-7079 JJ denotes dramatic
T9425 7080-7088 NN denotes increase
T9427 7089-7091 IN denotes in
T9428 7092-7097 NN denotes urine
T9429 7098-7111 NN denotes concentration
T9430 7111-7113 , denotes ,
T9431 7113-7117 IN denotes from
T9432 7118-7123 CD denotes 1,293
T9433 7124-7126 IN denotes to
T9434 7127-7132 CD denotes 5,885
T9435 7133-7137 NN denotes mOsm
T9436 7138-7139 -LRB- denotes (
T9438 7139-7147 RB denotes 4.6-fold
T9439 7147-7148 : denotes ;
T9437 7149-7155 NN denotes Figure
T9440 7156-7158 CD denotes 1D
T9441 7158-7159 -RRB- denotes )
T9442 7159-7160 . denotes .
T9443 7160-7432 sentence denotes With similar treatment, mutant mice concentrate their urine to a lesser but still significant extent, from 161 to 470 mOsm (2.9-fold), indicating that these animals are not only unable to concentrate their urine properly but are also defective in their response to dDAVP.
T9444 7161-7165 IN denotes With
T9446 7166-7173 JJ denotes similar
T9447 7174-7183 NN denotes treatment
T9448 7183-7185 , denotes ,
T9449 7185-7191 NN denotes mutant
T9450 7192-7196 NNS denotes mice
T9445 7197-7208 VBP denotes concentrate
T9451 7209-7214 PRP$ denotes their
T9452 7215-7220 NN denotes urine
T9453 7221-7223 IN denotes to
T9454 7224-7225 DT denotes a
T9456 7226-7232 JJR denotes lesser
T9457 7233-7236 CC denotes but
T9458 7237-7242 RB denotes still
T9459 7243-7254 JJ denotes significant
T9455 7255-7261 NN denotes extent
T9460 7261-7263 , denotes ,
T9461 7263-7267 IN denotes from
T9462 7268-7271 CD denotes 161
T9463 7272-7274 IN denotes to
T9464 7275-7278 CD denotes 470
T9465 7279-7283 NN denotes mOsm
T9466 7284-7285 -LRB- denotes (
T9467 7285-7293 RB denotes 2.9-fold
T9468 7293-7294 -RRB- denotes )
T9469 7294-7296 , denotes ,
T9470 7296-7306 VBG denotes indicating
T9471 7307-7311 IN denotes that
T9473 7312-7317 DT denotes these
T9474 7318-7325 NNS denotes animals
T9472 7326-7329 VBP denotes are
T9475 7330-7333 RB denotes not
T9476 7334-7338 RB denotes only
T9477 7339-7345 JJ denotes unable
T9478 7346-7348 TO denotes to
T9479 7349-7360 VB denotes concentrate
T9480 7361-7366 PRP$ denotes their
T9481 7367-7372 NN denotes urine
T9482 7373-7381 RB denotes properly
T9483 7382-7385 CC denotes but
T9484 7386-7389 VBP denotes are
T9485 7390-7394 RB denotes also
T9486 7395-7404 JJ denotes defective
T9487 7405-7407 IN denotes in
T9488 7408-7413 PRP$ denotes their
T9489 7414-7422 NN denotes response
T9490 7423-7425 IN denotes to
T9491 7426-7431 NN denotes dDAVP
T9492 7431-7432 . denotes .
T9493 7432-7631 sentence denotes The smaller response to dDAVP indicates some residual activity of the mutant AQP2 channel, which must be sufficient to allow survival of the individual, in contrast to the T126M knock-in mouse [18].
T9494 7433-7436 DT denotes The
T9496 7437-7444 JJR denotes smaller
T9495 7445-7453 NN denotes response
T9498 7454-7456 IN denotes to
T9499 7457-7462 NN denotes dDAVP
T9497 7463-7472 VBZ denotes indicates
T9500 7473-7477 DT denotes some
T9502 7478-7486 JJ denotes residual
T9501 7487-7495 NN denotes activity
T9503 7496-7498 IN denotes of
T9504 7499-7502 DT denotes the
T9506 7503-7509 NN denotes mutant
T9507 7510-7514 NN denotes AQP2
T9505 7515-7522 NN denotes channel
T9508 7522-7524 , denotes ,
T9509 7524-7529 WDT denotes which
T9511 7530-7534 MD denotes must
T9510 7535-7537 VB denotes be
T9512 7538-7548 JJ denotes sufficient
T9513 7549-7551 TO denotes to
T9514 7552-7557 VB denotes allow
T9515 7558-7566 NN denotes survival
T9516 7567-7569 IN denotes of
T9517 7570-7573 DT denotes the
T9518 7574-7584 NN denotes individual
T9519 7584-7586 , denotes ,
T9520 7586-7588 IN denotes in
T9521 7589-7597 NN denotes contrast
T9522 7598-7600 IN denotes to
T9523 7601-7604 DT denotes the
T9525 7605-7610 NN denotes T126M
T9526 7611-7616 VB denotes knock
T9527 7616-7617 HYPH denotes -
T9528 7617-7619 RP denotes in
T9524 7620-7625 NN denotes mouse
T9529 7626-7627 -LRB- denotes [
T9530 7627-7629 CD denotes 18
T9531 7629-7630 -RRB- denotes ]
T9532 7630-7631 . denotes .
T9533 7631-7889 sentence denotes Multiple heterozygous matings yielded 101 animals, which appeared at a ratio of 26:49:26, near the expected Mendelian wild type, heterozygote, and mutant frequencies, respectively, indicating that there is no reduced viability associated with this mutation.
T9534 7632-7640 JJ denotes Multiple
T9536 7641-7653 JJ denotes heterozygous
T9535 7654-7661 NNS denotes matings
T9537 7662-7669 VBD denotes yielded
T9538 7670-7673 CD denotes 101
T9539 7674-7681 NNS denotes animals
T9540 7681-7683 , denotes ,
T9541 7683-7688 WDT denotes which
T9542 7689-7697 VBD denotes appeared
T9543 7698-7700 IN denotes at
T9544 7701-7702 DT denotes a
T9545 7703-7708 NN denotes ratio
T9546 7709-7711 IN denotes of
T9547 7712-7714 CD denotes 26
T9548 7714-7715 SYM denotes :
T9549 7715-7717 CD denotes 49
T9550 7717-7718 SYM denotes :
T9551 7718-7720 CD denotes 26
T9552 7720-7722 , denotes ,
T9553 7722-7726 IN denotes near
T9554 7727-7730 DT denotes the
T9556 7731-7739 VBN denotes expected
T9557 7740-7749 JJ denotes Mendelian
T9559 7750-7754 JJ denotes wild
T9558 7755-7759 NN denotes type
T9560 7759-7761 , denotes ,
T9561 7761-7773 NN denotes heterozygote
T9562 7773-7775 , denotes ,
T9563 7775-7778 CC denotes and
T9564 7779-7785 NN denotes mutant
T9555 7786-7797 NNS denotes frequencies
T9565 7797-7799 , denotes ,
T9566 7799-7811 RB denotes respectively
T9567 7811-7813 , denotes ,
T9568 7813-7823 VBG denotes indicating
T9569 7824-7828 IN denotes that
T9571 7829-7834 EX denotes there
T9570 7835-7837 VBZ denotes is
T9572 7838-7840 DT denotes no
T9574 7841-7848 VBN denotes reduced
T9573 7849-7858 NN denotes viability
T9575 7859-7869 VBN denotes associated
T9576 7870-7874 IN denotes with
T9577 7875-7879 DT denotes this
T9578 7880-7888 NN denotes mutation
T9579 7888-7889 . denotes .
T9580 7889-8072 sentence denotes Other than the increased urine production and water intake, there was no overt phenotype in mutant mice, save distended kidneys, which appeared variably in adult animals (Figure 2A).
T9581 7890-7895 JJ denotes Other
T9583 7896-7900 IN denotes than
T9584 7901-7904 DT denotes the
T9586 7905-7914 VBN denotes increased
T9587 7915-7920 NN denotes urine
T9585 7921-7931 NN denotes production
T9588 7932-7935 CC denotes and
T9589 7936-7941 NN denotes water
T9590 7942-7948 NN denotes intake
T9591 7948-7950 , denotes ,
T9592 7950-7955 EX denotes there
T9582 7956-7959 VBD denotes was
T9593 7960-7962 DT denotes no
T9595 7963-7968 JJ denotes overt
T9594 7969-7978 NN denotes phenotype
T9596 7979-7981 IN denotes in
T9597 7982-7988 NN denotes mutant
T9598 7989-7993 NNS denotes mice
T9599 7993-7995 , denotes ,
T9600 7995-7999 IN denotes save
T9601 8000-8009 JJ denotes distended
T9602 8010-8017 NNS denotes kidneys
T9603 8017-8019 , denotes ,
T9604 8019-8024 WDT denotes which
T9605 8025-8033 VBD denotes appeared
T9606 8034-8042 RB denotes variably
T9607 8043-8045 IN denotes in
T9608 8046-8051 JJ denotes adult
T9609 8052-8059 NNS denotes animals
T9610 8060-8061 -LRB- denotes (
T9611 8061-8067 NN denotes Figure
T9612 8068-8070 CD denotes 2A
T9613 8070-8071 -RRB- denotes )
T9614 8071-8072 . denotes .
T9615 8072-8152 sentence denotes Although not specifically measured, mutant mice seem to have a normal lifespan.
T9616 8073-8081 IN denotes Although
T9618 8082-8085 RB denotes not
T9619 8086-8098 RB denotes specifically
T9617 8099-8107 VBN denotes measured
T9621 8107-8109 , denotes ,
T9622 8109-8115 NN denotes mutant
T9623 8116-8120 NNS denotes mice
T9620 8121-8125 VBP denotes seem
T9624 8126-8128 TO denotes to
T9625 8129-8133 VB denotes have
T9626 8134-8135 DT denotes a
T9628 8136-8142 JJ denotes normal
T9627 8143-8151 NN denotes lifespan
T9629 8151-8152 . denotes .
T9630 8152-8236 sentence denotes The one animal that was followed lived to 18 mo, typical for animals in our colony.
T9631 8153-8156 DT denotes The
T9633 8157-8160 CD denotes one
T9632 8161-8167 NN denotes animal
T9635 8168-8172 WDT denotes that
T9637 8173-8176 VBD denotes was
T9636 8177-8185 VBN denotes followed
T9634 8186-8191 VBN denotes lived
T9638 8192-8194 IN denotes to
T9639 8195-8197 CD denotes 18
T9640 8198-8200 NNS denotes mo
T9641 8200-8202 , denotes ,
T9642 8202-8209 JJ denotes typical
T9643 8210-8213 IN denotes for
T9644 8214-8221 NNS denotes animals
T9645 8222-8224 IN denotes in
T9646 8225-8228 PRP$ denotes our
T9647 8229-8235 NN denotes colony
T9648 8235-8236 . denotes .
T9649 8236-9234 sentence denotes Figure 2 Anatomy and Histology of Mouse Kidneys (A) Gross anatomy of an affected mouse (8-mo-old male). This shows the enlargement and cystic dilatation of the renal pelvis. There is thinning of the overlying renal parenchyma imparting a translucent appearance to portions of the kidney and collecting system. The bladder is also dilated. (B) Left kidney from mutant mouse (right) shown in (A) compared to a kidney from an age-sex matched unaffected littermate (left). (C) Hematoxylin and eosin stained section of ureter from a mutant mouse, showing normal histology despite bloating of the kidney. (D) Hematoxylin and eosin stained histologic section of a kidney from a 4-wk-old female mutant mouse. The mutant kidney shows marked dilatation of the renal pelvis with blunting of the papilla. There is preservation of the cortex and medulla. Aqp2F204V/F204V mice suffer from severe hydronephrosis (Figure 2A and 2B), presumably as a consequence of an inability to cope with the extreme polyuria.
T9650 9081-9090 NN denotes Aqp2F204V
T9652 9090-9091 HYPH denotes /
T9651 9091-9096 NN denotes F204V
T9653 9097-9101 NNS denotes mice
T9654 9102-9108 VBP denotes suffer
T9655 9109-9113 IN denotes from
T9656 9114-9120 JJ denotes severe
T9657 9121-9135 NN denotes hydronephrosis
T9658 9136-9137 -LRB- denotes (
T9660 9137-9143 NN denotes Figure
T9659 9144-9146 CD denotes 2A
T9661 9147-9150 CC denotes and
T9662 9151-9153 CD denotes 2B
T9663 9153-9154 -RRB- denotes )
T9664 9154-9156 , denotes ,
T9665 9156-9166 RB denotes presumably
T9666 9167-9169 IN denotes as
T9667 9170-9171 DT denotes a
T9668 9172-9183 NN denotes consequence
T9669 9184-9186 IN denotes of
T9670 9187-9189 DT denotes an
T9671 9190-9199 NN denotes inability
T9672 9200-9202 TO denotes to
T9673 9203-9207 VB denotes cope
T9674 9208-9212 IN denotes with
T9675 9213-9216 DT denotes the
T9677 9217-9224 JJ denotes extreme
T9676 9225-9233 NN denotes polyuria
T9678 9233-9234 . denotes .
T9679 9234-9376 sentence denotes We found distended kidneys in all Aqp2F204V/F204V mice; however, the degree of inflation was variable in affected mice and worsened with age.
T9680 9235-9237 PRP denotes We
T9681 9238-9243 VBD denotes found
T9683 9244-9253 JJ denotes distended
T9684 9254-9261 NNS denotes kidneys
T9685 9262-9264 IN denotes in
T9686 9265-9268 DT denotes all
T9688 9269-9278 NN denotes Aqp2F204V
T9690 9278-9279 HYPH denotes /
T9689 9279-9284 NN denotes F204V
T9687 9285-9289 NNS denotes mice
T9691 9289-9290 : denotes ;
T9692 9291-9298 RB denotes however
T9693 9298-9300 , denotes ,
T9694 9300-9303 DT denotes the
T9695 9304-9310 NN denotes degree
T9696 9311-9313 IN denotes of
T9697 9314-9323 NN denotes inflation
T9682 9324-9327 VBD denotes was
T9698 9328-9336 JJ denotes variable
T9699 9337-9339 IN denotes in
T9700 9340-9348 VBN denotes affected
T9701 9349-9353 NNS denotes mice
T9702 9354-9357 CC denotes and
T9703 9358-9366 VBD denotes worsened
T9704 9367-9371 IN denotes with
T9705 9372-9375 NN denotes age
T9706 9375-9376 . denotes .
T9707 9376-9489 sentence denotes Severe hydronephrosis has previously been observed in double Aqp1/Aqp3 knock-out mice [17], and appears at 6 wk.
T9708 9377-9383 JJ denotes Severe
T9709 9384-9398 NN denotes hydronephrosis
T9711 9399-9402 VBZ denotes has
T9712 9403-9413 RB denotes previously
T9713 9414-9418 VBN denotes been
T9710 9419-9427 VBN denotes observed
T9714 9428-9430 IN denotes in
T9715 9431-9437 JJ denotes double
T9717 9438-9442 NN denotes Aqp1
T9719 9442-9443 HYPH denotes /
T9718 9443-9447 NN denotes Aqp3
T9716 9448-9453 VB denotes knock
T9721 9453-9454 HYPH denotes -
T9722 9454-9457 RP denotes out
T9720 9458-9462 NNS denotes mice
T9723 9463-9464 -LRB- denotes [
T9724 9464-9466 CD denotes 17
T9725 9466-9467 -RRB- denotes ]
T9726 9467-9469 , denotes ,
T9727 9469-9472 CC denotes and
T9728 9473-9480 VBZ denotes appears
T9729 9481-9483 IN denotes at
T9730 9484-9485 CD denotes 6
T9731 9486-9488 NNS denotes wk
T9732 9488-9489 . denotes .
T9733 9489-9544 sentence denotes Even at 4 wk, Aqp2F204V/F204V mice had hydronephrosis.
T9734 9490-9494 RB denotes Even
T9735 9495-9497 IN denotes at
T9737 9498-9499 CD denotes 4
T9738 9500-9502 NNS denotes wk
T9739 9502-9504 , denotes ,
T9740 9504-9513 NN denotes Aqp2F204V
T9742 9513-9514 HYPH denotes /
T9741 9514-9519 NN denotes F204V
T9743 9520-9524 NNS denotes mice
T9736 9525-9528 VBD denotes had
T9744 9529-9543 NN denotes hydronephrosis
T9745 9543-9544 . denotes .
T9746 9544-9697 sentence denotes Histologic sections from Aqp2F204V/F204V mice demonstrated marked dilatation of the renal pelvis yet normal morphology of the ureter (Figure 2C and 2D).
T9747 9545-9555 JJ denotes Histologic
T9748 9556-9564 NNS denotes sections
T9750 9565-9569 IN denotes from
T9751 9570-9579 NN denotes Aqp2F204V
T9753 9579-9580 HYPH denotes /
T9752 9580-9585 NN denotes F204V
T9754 9586-9590 NNS denotes mice
T9749 9591-9603 VBD denotes demonstrated
T9755 9604-9610 JJ denotes marked
T9756 9611-9621 NN denotes dilatation
T9757 9622-9624 IN denotes of
T9758 9625-9628 DT denotes the
T9760 9629-9634 JJ denotes renal
T9759 9635-9641 NN denotes pelvis
T9761 9642-9645 CC denotes yet
T9762 9646-9652 JJ denotes normal
T9763 9653-9663 NN denotes morphology
T9764 9664-9666 IN denotes of
T9765 9667-9670 DT denotes the
T9766 9671-9677 NN denotes ureter
T9767 9678-9679 -LRB- denotes (
T9769 9679-9685 NN denotes Figure
T9768 9686-9688 CD denotes 2C
T9770 9689-9692 CC denotes and
T9771 9693-9695 CD denotes 2D
T9772 9695-9696 -RRB- denotes )
T9773 9696-9697 . denotes .
T9774 9697-9774 sentence denotes In particular, the muscularis propria was neither hypertrophied nor thinned.
T9775 9698-9700 IN denotes In
T9777 9701-9711 JJ denotes particular
T9778 9711-9713 , denotes ,
T9779 9713-9716 DT denotes the
T9781 9717-9727 NN denotes muscularis
T9780 9728-9735 NN denotes propria
T9776 9736-9739 VBD denotes was
T9782 9740-9747 CC denotes neither
T9783 9748-9761 JJ denotes hypertrophied
T9784 9762-9765 CC denotes nor
T9785 9766-9773 JJ denotes thinned
T9786 9773-9774 . denotes .
T9787 9774-9893 sentence denotes There was the normal festooned appearance of the urothelium, and this transitional epithelium was of normal thickness.
T9788 9775-9780 EX denotes There
T9789 9781-9784 VBD denotes was
T9790 9785-9788 DT denotes the
T9792 9789-9795 JJ denotes normal
T9793 9796-9805 VBN denotes festooned
T9791 9806-9816 NN denotes appearance
T9794 9817-9819 IN denotes of
T9795 9820-9823 DT denotes the
T9796 9824-9834 NN denotes urothelium
T9797 9834-9836 , denotes ,
T9798 9836-9839 CC denotes and
T9799 9840-9844 DT denotes this
T9801 9845-9857 JJ denotes transitional
T9800 9858-9868 NN denotes epithelium
T9802 9869-9872 VBD denotes was
T9803 9873-9875 IN denotes of
T9804 9876-9882 JJ denotes normal
T9805 9883-9892 NN denotes thickness
T9806 9892-9893 . denotes .
T9807 9893-9974 sentence denotes There was thinning of the kidney as measured from renal capsule to renal pelvis.
T9808 9894-9899 EX denotes There
T9809 9900-9903 VBD denotes was
T9810 9904-9912 NN denotes thinning
T9811 9913-9915 IN denotes of
T9812 9916-9919 DT denotes the
T9813 9920-9926 NN denotes kidney
T9814 9927-9929 IN denotes as
T9815 9930-9938 VBN denotes measured
T9816 9939-9943 IN denotes from
T9817 9944-9949 JJ denotes renal
T9818 9950-9957 NN denotes capsule
T9819 9958-9960 IN denotes to
T9820 9961-9966 JJ denotes renal
T9821 9967-9973 NN denotes pelvis
T9822 9973-9974 . denotes .
T9823 9974-10084 sentence denotes However, the morphologic features of the glomeruli and proximal/distal tubules were unremarkable (Figure 2D).
T9824 9975-9982 RB denotes However
T9826 9982-9984 , denotes ,
T9827 9984-9987 DT denotes the
T9829 9988-9999 JJ denotes morphologic
T9828 10000-10008 NNS denotes features
T9830 10009-10011 IN denotes of
T9831 10012-10015 DT denotes the
T9832 10016-10025 NNS denotes glomeruli
T9833 10026-10029 CC denotes and
T9834 10030-10038 JJ denotes proximal
T9836 10038-10039 HYPH denotes /
T9835 10039-10045 JJ denotes distal
T9837 10046-10053 NNS denotes tubules
T9825 10054-10058 VBD denotes were
T9838 10059-10071 JJ denotes unremarkable
T9839 10072-10073 -LRB- denotes (
T9840 10073-10079 NN denotes Figure
T9841 10080-10082 CD denotes 2D
T9842 10082-10083 -RRB- denotes )
T9843 10083-10084 . denotes .
T9844 10084-10233 sentence denotes As shown previously [18,22], immunoblotting revealed three different forms of AQP2, due to different degrees and forms of glycosylation (Figure 3A).
T9845 10085-10087 IN denotes As
T9846 10088-10093 VBN denotes shown
T9848 10094-10104 RB denotes previously
T9849 10105-10106 -LRB- denotes [
T9851 10106-10108 CD denotes 18
T9852 10108-10109 , denotes ,
T9850 10109-10111 CD denotes 22
T9853 10111-10112 -RRB- denotes ]
T9854 10112-10114 , denotes ,
T9855 10114-10128 NN denotes immunoblotting
T9847 10129-10137 VBD denotes revealed
T9856 10138-10143 CD denotes three
T9858 10144-10153 JJ denotes different
T9857 10154-10159 NNS denotes forms
T9859 10160-10162 IN denotes of
T9860 10163-10167 NN denotes AQP2
T9861 10167-10169 , denotes ,
T9862 10169-10172 IN denotes due
T9863 10173-10175 IN denotes to
T9864 10176-10185 JJ denotes different
T9865 10186-10193 NNS denotes degrees
T9866 10194-10197 CC denotes and
T9867 10198-10203 NNS denotes forms
T9868 10204-10206 IN denotes of
T9869 10207-10220 NN denotes glycosylation
T9870 10221-10222 -LRB- denotes (
T9871 10222-10228 NN denotes Figure
T9872 10229-10231 CD denotes 3A
T9873 10231-10232 -RRB- denotes )
T9874 10232-10233 . denotes .
T9875 10233-10397 sentence denotes Previous reports have demonstrated that nonglycosylated protein appears as a 29 kDa band, while complex glycosylated protein runs as a smear between 35 and 45 kDa.
T9876 10234-10242 JJ denotes Previous
T9877 10243-10250 NNS denotes reports
T9879 10251-10255 VBP denotes have
T9878 10256-10268 VBN denotes demonstrated
T9880 10269-10273 IN denotes that
T9882 10274-10289 JJ denotes nonglycosylated
T9883 10290-10297 NN denotes protein
T9881 10298-10305 VBZ denotes appears
T9884 10306-10308 IN denotes as
T9885 10309-10310 DT denotes a
T9887 10311-10313 CD denotes 29
T9888 10314-10317 NN denotes kDa
T9886 10318-10322 NN denotes band
T9889 10322-10324 , denotes ,
T9890 10324-10329 IN denotes while
T9892 10330-10337 JJ denotes complex
T9894 10338-10350 VBN denotes glycosylated
T9893 10351-10358 NN denotes protein
T9891 10359-10363 VBZ denotes runs
T9895 10364-10366 IN denotes as
T9896 10367-10368 DT denotes a
T9897 10369-10374 NN denotes smear
T9898 10375-10382 IN denotes between
T9899 10383-10385 CD denotes 35
T9901 10386-10389 CC denotes and
T9902 10390-10392 CD denotes 45
T9900 10393-10396 NN denotes kDa
T9903 10396-10397 . denotes .
T9904 10397-10549 sentence denotes A short-lived intermediate form of 31 kDa representing core, high-mannose glycosylation of AQP2 is apparent from pulse-chase labeling experiments [22].
T9905 10398-10399 DT denotes A
T9907 10400-10405 JJ denotes short
T9909 10405-10406 HYPH denotes -
T9908 10406-10411 JJ denotes lived
T9910 10412-10424 JJ denotes intermediate
T9906 10425-10429 NN denotes form
T9912 10430-10432 IN denotes of
T9913 10433-10435 CD denotes 31
T9914 10436-10439 NN denotes kDa
T9915 10440-10452 VBG denotes representing
T9916 10453-10457 NN denotes core
T9918 10457-10459 , denotes ,
T9919 10459-10463 JJ denotes high
T9921 10463-10464 HYPH denotes -
T9920 10464-10471 NN denotes mannose
T9917 10472-10485 NN denotes glycosylation
T9922 10486-10488 IN denotes of
T9923 10489-10493 NN denotes AQP2
T9911 10494-10496 VBZ denotes is
T9924 10497-10505 JJ denotes apparent
T9925 10506-10510 IN denotes from
T9926 10511-10516 NN denotes pulse
T9928 10516-10517 HYPH denotes -
T9927 10517-10522 NN denotes chase
T9929 10523-10531 NN denotes labeling
T9930 10532-10543 NNS denotes experiments
T9931 10544-10545 -LRB- denotes [
T9932 10545-10547 CD denotes 22
T9933 10547-10548 -RRB- denotes ]
T9934 10548-10549 . denotes .
T9935 10549-10796 sentence denotes Compared to that from the kidneys of wild-type animals, AQP2 from mutant animals was reduced in both the high molecular weight, diffuse form and the lowest molecular weight form, but enriched in the intermediate molecular weight form (Figure 3A).
T9936 10550-10558 VBN denotes Compared
T9938 10559-10561 IN denotes to
T9939 10562-10566 DT denotes that
T9940 10567-10571 IN denotes from
T9941 10572-10575 DT denotes the
T9942 10576-10583 NNS denotes kidneys
T9943 10584-10586 IN denotes of
T9944 10587-10591 JJ denotes wild
T9946 10591-10592 HYPH denotes -
T9945 10592-10596 NN denotes type
T9947 10597-10604 NNS denotes animals
T9948 10604-10606 , denotes ,
T9949 10606-10610 NN denotes AQP2
T9950 10611-10615 IN denotes from
T9951 10616-10622 NN denotes mutant
T9952 10623-10630 NNS denotes animals
T9953 10631-10634 VBD denotes was
T9937 10635-10642 VBN denotes reduced
T9954 10643-10645 IN denotes in
T9955 10646-10650 CC denotes both
T9957 10651-10654 DT denotes the
T9958 10655-10659 JJ denotes high
T9960 10660-10669 JJ denotes molecular
T9959 10670-10676 NN denotes weight
T9961 10676-10678 , denotes ,
T9962 10678-10685 JJ denotes diffuse
T9956 10686-10690 NN denotes form
T9963 10691-10694 CC denotes and
T9964 10695-10698 DT denotes the
T9966 10699-10705 JJS denotes lowest
T9968 10706-10715 JJ denotes molecular
T9967 10716-10722 NN denotes weight
T9965 10723-10727 NN denotes form
T9969 10727-10729 , denotes ,
T9970 10729-10732 CC denotes but
T9971 10733-10741 VBN denotes enriched
T9972 10742-10744 IN denotes in
T9973 10745-10748 DT denotes the
T9975 10749-10761 JJ denotes intermediate
T9977 10762-10771 JJ denotes molecular
T9976 10772-10778 NN denotes weight
T9974 10779-10783 NN denotes form
T9978 10784-10785 -LRB- denotes (
T9979 10785-10791 NN denotes Figure
T9980 10792-10794 CD denotes 3A
T9981 10794-10795 -RRB- denotes )
T9982 10795-10796 . denotes .
T9983 10796-10865 sentence denotes Heterozygous animals showed intermediate amounts of all three forms.
T9984 10797-10809 JJ denotes Heterozygous
T9985 10810-10817 NNS denotes animals
T9986 10818-10824 VBD denotes showed
T9987 10825-10837 JJ denotes intermediate
T9988 10838-10845 NNS denotes amounts
T9989 10846-10848 IN denotes of
T9990 10849-10852 DT denotes all
T9992 10853-10858 CD denotes three
T9991 10859-10864 NNS denotes forms
T9993 10864-10865 . denotes .
T9994 10865-11034 sentence denotes The nature of these glycosylated forms was revealed by digestion with endoglycosidase H, which specifically cleaves mannose-rich carbohydrate from the protein backbone.
T9995 10866-10869 DT denotes The
T9996 10870-10876 NN denotes nature
T9998 10877-10879 IN denotes of
T9999 10880-10885 DT denotes these
T10001 10886-10898 VBN denotes glycosylated
T10000 10899-10904 NNS denotes forms
T10002 10905-10908 VBD denotes was
T9997 10909-10917 VBN denotes revealed
T10003 10918-10920 IN denotes by
T10004 10921-10930 NN denotes digestion
T10005 10931-10935 IN denotes with
T10006 10936-10951 NN denotes endoglycosidase
T10007 10952-10953 NN denotes H
T10008 10953-10955 , denotes ,
T10009 10955-10960 WDT denotes which
T10011 10961-10973 RB denotes specifically
T10010 10974-10981 VBZ denotes cleaves
T10012 10982-10989 NN denotes mannose
T10014 10989-10990 HYPH denotes -
T10013 10990-10994 JJ denotes rich
T10015 10995-11007 NN denotes carbohydrate
T10016 11008-11012 IN denotes from
T10017 11013-11016 DT denotes the
T10019 11017-11024 NN denotes protein
T10018 11025-11033 NN denotes backbone
T10020 11033-11034 . denotes .
T10021 11034-11197 sentence denotes Treatment of endogenous AQP2 from kidneys of wild-type, heterozygous, and mutant animals specifically affected the intermediate molecular weight form (Figure 3B).
T10022 11035-11044 NN denotes Treatment
T10024 11045-11047 IN denotes of
T10025 11048-11058 JJ denotes endogenous
T10026 11059-11063 NN denotes AQP2
T10027 11064-11068 IN denotes from
T10028 11069-11076 NNS denotes kidneys
T10029 11077-11079 IN denotes of
T10030 11080-11084 JJ denotes wild
T10032 11084-11085 HYPH denotes -
T10031 11085-11089 NN denotes type
T10034 11089-11091 , denotes ,
T10035 11091-11103 JJ denotes heterozygous
T10036 11103-11105 , denotes ,
T10037 11105-11108 CC denotes and
T10038 11109-11115 JJ denotes mutant
T10033 11116-11123 NNS denotes animals
T10039 11124-11136 RB denotes specifically
T10023 11137-11145 VBD denotes affected
T10040 11146-11149 DT denotes the
T10042 11150-11162 JJ denotes intermediate
T10044 11163-11172 JJ denotes molecular
T10043 11173-11179 NN denotes weight
T10041 11180-11184 NN denotes form
T10045 11185-11186 -LRB- denotes (
T10046 11186-11192 NN denotes Figure
T10047 11193-11195 CD denotes 3B
T10048 11195-11196 -RRB- denotes )
T10049 11196-11197 . denotes .
T10050 11197-11406 sentence denotes The presence of some mature glycosylated proteins (35–45 kDa) in Aqp2F204V/F204V mice presumably permits their survival compared to Aqp2T126M/T126M mice, and is consistent with a diminished response to dDAVP.
T10051 11198-11201 DT denotes The
T10052 11202-11210 NN denotes presence
T10054 11211-11213 IN denotes of
T10055 11214-11218 DT denotes some
T10057 11219-11225 JJ denotes mature
T10058 11226-11238 VBN denotes glycosylated
T10056 11239-11247 NN denotes proteins
T10059 11248-11249 -LRB- denotes (
T10061 11249-11251 CD denotes 35
T10063 11251-11252 SYM denotes –
T10062 11252-11254 CD denotes 45
T10060 11255-11258 NN denotes kDa
T10064 11258-11259 -RRB- denotes )
T10065 11260-11262 IN denotes in
T10066 11263-11272 NN denotes Aqp2F204V
T10068 11272-11273 HYPH denotes /
T10067 11273-11278 NN denotes F204V
T10069 11279-11283 NNS denotes mice
T10070 11284-11294 RB denotes presumably
T10053 11295-11302 VBZ denotes permits
T10071 11303-11308 PRP$ denotes their
T10072 11309-11317 NN denotes survival
T10073 11318-11326 VBN denotes compared
T10074 11327-11329 IN denotes to
T10075 11330-11339 NN denotes Aqp2T126M
T10077 11339-11340 HYPH denotes /
T10076 11340-11345 NN denotes T126M
T10078 11346-11350 NNS denotes mice
T10079 11350-11352 , denotes ,
T10080 11352-11355 CC denotes and
T10081 11356-11358 VBZ denotes is
T10082 11359-11369 JJ denotes consistent
T10083 11370-11374 IN denotes with
T10084 11375-11376 DT denotes a
T10086 11377-11387 JJ denotes diminished
T10085 11388-11396 NN denotes response
T10087 11397-11399 IN denotes to
T10088 11400-11405 NN denotes dDAVP
T10089 11405-11406 . denotes .
T10090 11406-12060 sentence denotes Figure 3 Immunoblot Analyses of AQP2 from Mouse Kidneys (A) Western blot analyses of total kidney membranes from littermate mice. An intermediate form of AQP2 at 31 kDa was identified in kidney membranes from a mutant mouse (Mut) and partially in a heterozygous mouse (Het). (B) Total kidney membranes were subjected to endoglycosidase H treatment (Endo H) prior to Western blotting. High-mannose (h.m.) glycosylated proteins that have not exited the ER are sensitive to endoglycosidase H digestion. In humans, recessive alleles of Aqp2 are postulated to cause NDI because they do not properly translocate to the apical cell surface in response to AVP.
T10091 11908-11910 IN denotes In
T10093 11911-11917 NNS denotes humans
T10094 11917-11919 , denotes ,
T10095 11919-11928 JJ denotes recessive
T10096 11929-11936 NNS denotes alleles
T10097 11937-11939 IN denotes of
T10098 11940-11944 NN denotes Aqp2
T10099 11945-11948 VBP denotes are
T10092 11949-11959 VBN denotes postulated
T10100 11960-11962 TO denotes to
T10101 11963-11968 VB denotes cause
T10102 11969-11972 NN denotes NDI
T10103 11973-11980 IN denotes because
T10105 11981-11985 PRP denotes they
T10106 11986-11988 VBP denotes do
T10107 11989-11992 RB denotes not
T10108 11993-12001 RB denotes properly
T10104 12002-12013 VB denotes translocate
T10109 12014-12016 IN denotes to
T10110 12017-12020 DT denotes the
T10112 12021-12027 JJ denotes apical
T10113 12028-12032 NN denotes cell
T10111 12033-12040 NN denotes surface
T10114 12041-12043 IN denotes in
T10115 12044-12052 NN denotes response
T10116 12053-12055 IN denotes to
T10117 12056-12059 NN denotes AVP
T10118 12059-12060 . denotes .
T10119 12060-12218 sentence denotes This postulate comes solely from in vitro studies in which mutant Aqp2 cDNAs corresponding to human disease mutations are transfected into kidney cell lines.
T10120 12061-12065 DT denotes This
T10121 12066-12075 NN denotes postulate
T10122 12076-12081 VBZ denotes comes
T10123 12082-12088 RB denotes solely
T10124 12089-12093 IN denotes from
T10125 12094-12096 FW denotes in
T10126 12097-12102 FW denotes vitro
T10127 12103-12110 NNS denotes studies
T10128 12111-12113 IN denotes in
T10130 12114-12119 WDT denotes which
T10131 12120-12126 NN denotes mutant
T10133 12127-12131 NN denotes Aqp2
T10132 12132-12137 NNS denotes cDNAs
T10134 12138-12151 VBG denotes corresponding
T10135 12152-12154 IN denotes to
T10136 12155-12160 JJ denotes human
T10138 12161-12168 NN denotes disease
T10137 12169-12178 NNS denotes mutations
T10139 12179-12182 VBP denotes are
T10129 12183-12194 VBN denotes transfected
T10140 12195-12199 IN denotes into
T10141 12200-12206 NN denotes kidney
T10143 12207-12211 NN denotes cell
T10142 12212-12217 NNS denotes lines
T10144 12217-12218 . denotes .
T10145 12218-12337 sentence denotes In general, such recessive alleles, when visualized immunocytochemically, fail to localize to AVP-responsive vesicles.
T10146 12219-12221 IN denotes In
T10148 12222-12229 JJ denotes general
T10149 12229-12231 , denotes ,
T10150 12231-12235 JJ denotes such
T10152 12236-12245 JJ denotes recessive
T10151 12246-12253 NNS denotes alleles
T10153 12253-12255 , denotes ,
T10154 12255-12259 WRB denotes when
T10155 12260-12270 VBN denotes visualized
T10156 12271-12291 RB denotes immunocytochemically
T10157 12291-12293 , denotes ,
T10147 12293-12297 VBP denotes fail
T10158 12298-12300 TO denotes to
T10159 12301-12309 VB denotes localize
T10160 12310-12312 IN denotes to
T10161 12313-12316 NN denotes AVP
T10163 12316-12317 HYPH denotes -
T10162 12317-12327 JJ denotes responsive
T10164 12328-12336 NNS denotes vesicles
T10165 12336-12337 . denotes .
T10166 12337-12397 sentence denotes Rather, they get trapped in the endoplasmic reticulum (ER).
T10167 12338-12344 RB denotes Rather
T10169 12344-12346 , denotes ,
T10170 12346-12350 PRP denotes they
T10171 12351-12354 VBP denotes get
T10168 12355-12362 VBN denotes trapped
T10172 12363-12365 IN denotes in
T10173 12366-12369 DT denotes the
T10175 12370-12381 JJ denotes endoplasmic
T10174 12382-12391 NN denotes reticulum
T10176 12392-12393 -LRB- denotes (
T10177 12393-12395 NN denotes ER
T10178 12395-12396 -RRB- denotes )
T10179 12396-12397 . denotes .
T10180 12397-12494 sentence denotes Our mouse model of NDI affords the first opportunity to test this hypothesis in a mature animal.
T10181 12398-12401 PRP$ denotes Our
T10183 12402-12407 NN denotes mouse
T10182 12408-12413 NN denotes model
T10185 12414-12416 IN denotes of
T10186 12417-12420 NN denotes NDI
T10184 12421-12428 VBZ denotes affords
T10187 12429-12432 DT denotes the
T10189 12433-12438 JJ denotes first
T10188 12439-12450 NN denotes opportunity
T10190 12451-12453 TO denotes to
T10191 12454-12458 VB denotes test
T10192 12459-12463 DT denotes this
T10193 12464-12474 NN denotes hypothesis
T10194 12475-12477 IN denotes in
T10195 12478-12479 DT denotes a
T10197 12480-12486 JJ denotes mature
T10196 12487-12493 NN denotes animal
T10198 12493-12494 . denotes .
T10199 12494-12664 sentence denotes As shown in Figure 4A (top row of photomicrographs), AQP2 (stained red) normally localized to the subapical region of collecting duct cells in kidneys of wild-type mice.
T10200 12495-12497 IN denotes As
T10201 12498-12503 VBN denotes shown
T10203 12504-12506 IN denotes in
T10204 12507-12513 NN denotes Figure
T10205 12514-12516 CD denotes 4A
T10206 12517-12518 -LRB- denotes (
T10208 12518-12521 JJ denotes top
T10207 12522-12525 NN denotes row
T10209 12526-12528 IN denotes of
T10210 12529-12545 NNS denotes photomicrographs
T10211 12545-12546 -RRB- denotes )
T10212 12546-12548 , denotes ,
T10213 12548-12552 NN denotes AQP2
T10214 12553-12554 -LRB- denotes (
T10215 12554-12561 VBN denotes stained
T10216 12562-12565 JJ denotes red
T10217 12565-12566 -RRB- denotes )
T10218 12567-12575 RB denotes normally
T10202 12576-12585 VBD denotes localized
T10219 12586-12588 IN denotes to
T10220 12589-12592 DT denotes the
T10222 12593-12602 JJ denotes subapical
T10221 12603-12609 NN denotes region
T10223 12610-12612 IN denotes of
T10224 12613-12623 VBG denotes collecting
T10225 12624-12628 NN denotes duct
T10226 12629-12634 NNS denotes cells
T10227 12635-12637 IN denotes in
T10228 12638-12645 NNS denotes kidneys
T10229 12646-12648 IN denotes of
T10230 12649-12653 JJ denotes wild
T10232 12653-12654 HYPH denotes -
T10231 12654-12658 NN denotes type
T10233 12659-12663 NNS denotes mice
T10234 12663-12664 . denotes .
T10235 12664-12764 sentence denotes Upon stimulation with dDAVP, AQP2 translocated to or near the cell surface (Figure 4A, second row).
T10236 12665-12669 IN denotes Upon
T10238 12670-12681 NN denotes stimulation
T10239 12682-12686 IN denotes with
T10240 12687-12692 NN denotes dDAVP
T10241 12692-12694 , denotes ,
T10242 12694-12698 NN denotes AQP2
T10237 12699-12711 VBD denotes translocated
T10243 12712-12714 IN denotes to
T10244 12715-12717 CC denotes or
T10245 12718-12722 IN denotes near
T10246 12723-12726 DT denotes the
T10248 12727-12731 NN denotes cell
T10247 12732-12739 NN denotes surface
T10249 12740-12741 -LRB- denotes (
T10251 12741-12747 NN denotes Figure
T10252 12748-12750 CD denotes 4A
T10253 12750-12752 , denotes ,
T10254 12752-12758 JJ denotes second
T10250 12759-12762 NN denotes row
T10255 12762-12763 -RRB- denotes )
T10256 12763-12764 . denotes .
T10257 12764-12980 sentence denotes In kidneys taken from mutant animals, however, AQP2 was distributed randomly throughout the cell in the basal state (Figure 4A, third row), while AQP3 (green) appropriately localized to the basolateral surface [23].
T10258 12765-12767 IN denotes In
T10260 12768-12775 NNS denotes kidneys
T10261 12776-12781 VBN denotes taken
T10262 12782-12786 IN denotes from
T10263 12787-12793 NN denotes mutant
T10264 12794-12801 NNS denotes animals
T10265 12801-12803 , denotes ,
T10266 12803-12810 RB denotes however
T10267 12810-12812 , denotes ,
T10268 12812-12816 NN denotes AQP2
T10269 12817-12820 VBD denotes was
T10259 12821-12832 VBN denotes distributed
T10270 12833-12841 RB denotes randomly
T10271 12842-12852 IN denotes throughout
T10272 12853-12856 DT denotes the
T10273 12857-12861 NN denotes cell
T10274 12862-12864 IN denotes in
T10275 12865-12868 DT denotes the
T10277 12869-12874 JJ denotes basal
T10276 12875-12880 NN denotes state
T10278 12881-12882 -LRB- denotes (
T10280 12882-12888 NN denotes Figure
T10281 12889-12891 CD denotes 4A
T10282 12891-12893 , denotes ,
T10283 12893-12898 JJ denotes third
T10279 12899-12902 NN denotes row
T10284 12902-12903 -RRB- denotes )
T10285 12903-12905 , denotes ,
T10286 12905-12910 IN denotes while
T10288 12911-12915 NN denotes AQP3
T10289 12916-12917 -LRB- denotes (
T10290 12917-12922 NN denotes green
T10291 12922-12923 -RRB- denotes )
T10292 12924-12937 RB denotes appropriately
T10287 12938-12947 VBD denotes localized
T10293 12948-12950 IN denotes to
T10294 12951-12954 DT denotes the
T10296 12955-12966 JJ denotes basolateral
T10295 12967-12974 NN denotes surface
T10297 12975-12976 -LRB- denotes [
T10298 12976-12978 CD denotes 23
T10299 12978-12979 -RRB- denotes ]
T10300 12979-12980 . denotes .
T10301 12980-13095 sentence denotes Furthermore, upon dDAVP stimulation, AQP2-F204V failed to translocate to the cell surface (Figure 4A, bottom row).
T10302 12981-12992 RB denotes Furthermore
T10304 12992-12994 , denotes ,
T10305 12994-12998 IN denotes upon
T10306 12999-13004 NN denotes dDAVP
T10307 13005-13016 NN denotes stimulation
T10308 13016-13018 , denotes ,
T10309 13018-13022 NN denotes AQP2
T10311 13022-13023 HYPH denotes -
T10310 13023-13028 NN denotes F204V
T10303 13029-13035 VBD denotes failed
T10312 13036-13038 TO denotes to
T10313 13039-13050 VB denotes translocate
T10314 13051-13053 IN denotes to
T10315 13054-13057 DT denotes the
T10317 13058-13062 NN denotes cell
T10316 13063-13070 NN denotes surface
T10318 13071-13072 -LRB- denotes (
T10320 13072-13078 NN denotes Figure
T10321 13079-13081 CD denotes 4A
T10322 13081-13083 , denotes ,
T10323 13083-13089 JJ denotes bottom
T10319 13090-13093 NN denotes row
T10324 13093-13094 -RRB- denotes )
T10325 13094-13095 . denotes .
T10326 13095-13281 sentence denotes To confirm these findings, the staining was repeated in kidneys taken from two further mice for each class, wild-type or mutant, with or without dDAVP treatment, with identical results.
T10327 13096-13098 TO denotes To
T10328 13099-13106 VB denotes confirm
T10330 13107-13112 DT denotes these
T10331 13113-13121 NNS denotes findings
T10332 13121-13123 , denotes ,
T10333 13123-13126 DT denotes the
T10334 13127-13135 NN denotes staining
T10335 13136-13139 VBD denotes was
T10329 13140-13148 VBN denotes repeated
T10336 13149-13151 IN denotes in
T10337 13152-13159 NNS denotes kidneys
T10338 13160-13165 VBN denotes taken
T10339 13166-13170 IN denotes from
T10340 13171-13174 CD denotes two
T10342 13175-13182 JJ denotes further
T10341 13183-13187 NNS denotes mice
T10343 13188-13191 IN denotes for
T10344 13192-13196 DT denotes each
T10345 13197-13202 NN denotes class
T10346 13202-13204 , denotes ,
T10347 13204-13208 JJ denotes wild
T10349 13208-13209 HYPH denotes -
T10348 13209-13213 NN denotes type
T10350 13214-13216 CC denotes or
T10351 13217-13223 NN denotes mutant
T10352 13223-13225 , denotes ,
T10353 13225-13229 IN denotes with
T10354 13230-13232 CC denotes or
T10355 13233-13240 IN denotes without
T10356 13241-13246 NN denotes dDAVP
T10357 13247-13256 NN denotes treatment
T10358 13256-13258 , denotes ,
T10359 13258-13262 IN denotes with
T10360 13263-13272 JJ denotes identical
T10361 13273-13280 NNS denotes results
T10362 13280-13281 . denotes .
T10363 13281-15061 sentence denotes Figure 4 AQP2 Subcellular Localization and Translocation in Mouse Kidney Collecting Ducts and MDCK Cell Lines (A) Immunohistochemistry on collecting ducts in kidney sections from an AQP2-F204V mutant (Mut) mouse and an age-sex matched wild-type (WT) littermate. Mice were injected intraperitoneally with PBS (NT) or dDAVP before sacrificing and fixation of the kidneys. Kidneys sections were immunostained for AQP2 (red) and the basolateral marker AQP3 (green). The images were merged and an area of the cytoplasm was magnified (zoom). Note that mutant AQP2 is not properly localized to the subapical compartment, nor does it respond to dDAVP. (B) MDCK cell lines, stably transfected with constructs encoding mouse WT or AQP2-F204V, were treated with and without 150 μM forskolin for 90 min, after which cells were fixed, permeabilized, and subjected to immunocytochemistry. AQP2 is shown in green, and the basolateral marker Na+/K+-ATPase is shown in red, alongside the nuclear stain DAPI. The z-profile images were reconstructed from multiple z-sections, along the dotted line. Mutant AQP2 fails to localize to the cell surface upon forskolin stimulation. Rather, the perinuclear staining is consistent with an ER localization of mutant AQP2. (C) The MDCK cell line expressing AQP2-F204V was grown on fibronectin-coated coverslips until tight junctions formed, at which point the cells were treated with 150 μM forskolin for 90 min. Cells were fixed, permeabilized, and sequentially immunoblotted for AQP2 (green) and calnexin (red), an ER marker. The merged image shows that AQP2-F204V colocalizes with the endoplasmic reticulum marker. Scale bar refers to 10 μm. To investigate the mechanism of defective translocation of AQP2-F204V, we turned to transfection of MDCK cells.
T10364 14950-14952 TO denotes To
T10365 14953-14964 VB denotes investigate
T10367 14965-14968 DT denotes the
T10368 14969-14978 NN denotes mechanism
T10369 14979-14981 IN denotes of
T10370 14982-14991 JJ denotes defective
T10371 14992-15005 NN denotes translocation
T10372 15006-15008 IN denotes of
T10373 15009-15013 NN denotes AQP2
T10375 15013-15014 HYPH denotes -
T10374 15014-15019 NN denotes F204V
T10376 15019-15021 , denotes ,
T10377 15021-15023 PRP denotes we
T10366 15024-15030 VBD denotes turned
T10378 15031-15033 IN denotes to
T10379 15034-15046 NN denotes transfection
T10380 15047-15049 IN denotes of
T10381 15050-15054 NN denotes MDCK
T10382 15055-15060 NNS denotes cells
T10383 15060-15061 . denotes .
T10384 15061-15144 sentence denotes Stable cell lines expressing mouse wild-type AQP2 and AQP2-F204V were established.
T10385 15062-15068 JJ denotes Stable
T10387 15069-15073 NN denotes cell
T10386 15074-15079 NNS denotes lines
T10389 15080-15090 VBG denotes expressing
T10390 15091-15096 NN denotes mouse
T10392 15097-15101 JJ denotes wild
T10394 15101-15102 HYPH denotes -
T10393 15102-15106 NN denotes type
T10391 15107-15111 NN denotes AQP2
T10395 15112-15115 CC denotes and
T10396 15116-15120 NN denotes AQP2
T10398 15120-15121 HYPH denotes -
T10397 15121-15126 NN denotes F204V
T10399 15127-15131 VBD denotes were
T10388 15132-15143 VBN denotes established
T10400 15143-15144 . denotes .
T10401 15144-15300 sentence denotes Immunoblots of protein extracts from stable cell lines showed that MDCK cells recapitulate the glycosylation defect seen in mutant mice (unpublished data).
T10402 15145-15156 NNS denotes Immunoblots
T10404 15157-15159 IN denotes of
T10405 15160-15167 NN denotes protein
T10406 15168-15176 NNS denotes extracts
T10407 15177-15181 IN denotes from
T10408 15182-15188 JJ denotes stable
T10410 15189-15193 NN denotes cell
T10409 15194-15199 NNS denotes lines
T10403 15200-15206 VBD denotes showed
T10411 15207-15211 IN denotes that
T10413 15212-15216 NN denotes MDCK
T10414 15217-15222 NNS denotes cells
T10412 15223-15235 VBP denotes recapitulate
T10415 15236-15239 DT denotes the
T10417 15240-15253 NN denotes glycosylation
T10416 15254-15260 NN denotes defect
T10418 15261-15265 VBN denotes seen
T10419 15266-15268 IN denotes in
T10420 15269-15275 NN denotes mutant
T10421 15276-15280 NNS denotes mice
T10422 15281-15282 -LRB- denotes (
T10424 15282-15293 JJ denotes unpublished
T10423 15294-15298 NNS denotes data
T10425 15298-15299 -RRB- denotes )
T10426 15299-15300 . denotes .
T10427 15300-15366 sentence denotes The wild-type protein was again present in three different forms.
T10428 15301-15304 DT denotes The
T10430 15305-15309 JJ denotes wild
T10432 15309-15310 HYPH denotes -
T10431 15310-15314 NN denotes type
T10429 15315-15322 NN denotes protein
T10433 15323-15326 VBD denotes was
T10434 15327-15332 RB denotes again
T10435 15333-15340 JJ denotes present
T10436 15341-15343 IN denotes in
T10437 15344-15349 CD denotes three
T10439 15350-15359 JJ denotes different
T10438 15360-15365 NNS denotes forms
T10440 15365-15366 . denotes .
T10441 15366-15476 sentence denotes Cells expressing AQP2-F204V lacked the 35–45 kDa form and were enriched in the core-glycosylated 31 kDa form.
T10442 15367-15372 NNS denotes Cells
T10444 15373-15383 VBG denotes expressing
T10445 15384-15388 NN denotes AQP2
T10447 15388-15389 HYPH denotes -
T10446 15389-15394 NN denotes F204V
T10443 15395-15401 VBD denotes lacked
T10448 15402-15405 DT denotes the
T10450 15406-15408 CD denotes 35
T10452 15408-15409 SYM denotes –
T10451 15409-15411 CD denotes 45
T10453 15412-15415 NN denotes kDa
T10449 15416-15420 NN denotes form
T10454 15421-15424 CC denotes and
T10455 15425-15429 VBD denotes were
T10456 15430-15438 VBN denotes enriched
T10457 15439-15441 IN denotes in
T10458 15442-15445 DT denotes the
T10460 15446-15450 NN denotes core
T10462 15450-15451 HYPH denotes -
T10461 15451-15463 VBN denotes glycosylated
T10463 15464-15466 CD denotes 31
T10464 15467-15470 NN denotes kDa
T10459 15471-15475 NN denotes form
T10465 15475-15476 . denotes .
T10466 15476-15714 sentence denotes In transfected, unstimulated MDCK cells, wild-type AQP2 (stained green) appeared in a punctate pattern distributed throughout the subapical region (Figure 4B, left column photomicrographs), consistent with vesicular compartmentalization.
T10467 15477-15479 IN denotes In
T10469 15480-15491 VBN denotes transfected
T10471 15491-15493 , denotes ,
T10472 15493-15505 JJ denotes unstimulated
T10473 15506-15510 NN denotes MDCK
T10470 15511-15516 NNS denotes cells
T10474 15516-15518 , denotes ,
T10475 15518-15522 JJ denotes wild
T10477 15522-15523 HYPH denotes -
T10476 15523-15527 NN denotes type
T10478 15528-15532 NN denotes AQP2
T10479 15533-15534 -LRB- denotes (
T10480 15534-15541 VBN denotes stained
T10481 15542-15547 JJ denotes green
T10482 15547-15548 -RRB- denotes )
T10468 15549-15557 VBD denotes appeared
T10483 15558-15560 IN denotes in
T10484 15561-15562 DT denotes a
T10486 15563-15571 NN denotes punctate
T10485 15572-15579 NN denotes pattern
T10487 15580-15591 VBN denotes distributed
T10488 15592-15602 IN denotes throughout
T10489 15603-15606 DT denotes the
T10491 15607-15616 JJ denotes subapical
T10490 15617-15623 NN denotes region
T10492 15624-15625 -LRB- denotes (
T10494 15625-15631 NN denotes Figure
T10495 15632-15634 CD denotes 4B
T10496 15634-15636 , denotes ,
T10497 15636-15640 JJ denotes left
T10498 15641-15647 NN denotes column
T10493 15648-15664 NNS denotes photomicrographs
T10499 15664-15665 -RRB- denotes )
T10500 15665-15667 , denotes ,
T10501 15667-15677 JJ denotes consistent
T10502 15678-15682 IN denotes with
T10503 15683-15692 JJ denotes vesicular
T10504 15693-15713 NN denotes compartmentalization
T10505 15713-15714 . denotes .
T10506 15714-15819 sentence denotes AQP2-F204V, on the other hand, appeared in a punctate but perinuclear pattern (Figure 4B, third column).
T10507 15715-15719 NN denotes AQP2
T10509 15719-15720 HYPH denotes -
T10508 15720-15725 NN denotes F204V
T10511 15725-15727 , denotes ,
T10512 15727-15729 IN denotes on
T10513 15730-15733 DT denotes the
T10515 15734-15739 JJ denotes other
T10514 15740-15744 NN denotes hand
T10516 15744-15746 , denotes ,
T10510 15746-15754 VBD denotes appeared
T10517 15755-15757 IN denotes in
T10518 15758-15759 DT denotes a
T10520 15760-15768 NN denotes punctate
T10522 15769-15772 CC denotes but
T10521 15773-15784 JJ denotes perinuclear
T10519 15785-15792 NN denotes pattern
T10523 15793-15794 -LRB- denotes (
T10525 15794-15800 NN denotes Figure
T10526 15801-15803 CD denotes 4B
T10527 15803-15805 , denotes ,
T10528 15805-15810 JJ denotes third
T10524 15811-15817 NN denotes column
T10529 15817-15818 -RRB- denotes )
T10530 15818-15819 . denotes .
T10531 15819-15997 sentence denotes Upon stimulation with forskolin, a cAMP-dependent protein kinase activator, wild-type AQP2 translocated to the apical surface of polarized MDCK cells (Figure 4B, second column).
T10532 15820-15824 IN denotes Upon
T10534 15825-15836 NN denotes stimulation
T10535 15837-15841 IN denotes with
T10536 15842-15851 NN denotes forskolin
T10537 15851-15853 , denotes ,
T10538 15853-15854 DT denotes a
T10540 15855-15859 NN denotes cAMP
T10542 15859-15860 HYPH denotes -
T10541 15860-15869 JJ denotes dependent
T10543 15870-15877 NN denotes protein
T10544 15878-15884 NN denotes kinase
T10539 15885-15894 NN denotes activator
T10545 15894-15896 , denotes ,
T10546 15896-15900 JJ denotes wild
T10548 15900-15901 HYPH denotes -
T10547 15901-15905 NN denotes type
T10549 15906-15910 NN denotes AQP2
T10533 15911-15923 VBD denotes translocated
T10550 15924-15926 IN denotes to
T10551 15927-15930 DT denotes the
T10553 15931-15937 JJ denotes apical
T10552 15938-15945 NN denotes surface
T10554 15946-15948 IN denotes of
T10555 15949-15958 VBN denotes polarized
T10557 15959-15963 NN denotes MDCK
T10556 15964-15969 NNS denotes cells
T10558 15970-15971 -LRB- denotes (
T10560 15971-15977 NN denotes Figure
T10561 15978-15980 CD denotes 4B
T10562 15980-15982 , denotes ,
T10563 15982-15988 JJ denotes second
T10559 15989-15995 NN denotes column
T10564 15995-15996 -RRB- denotes )
T10565 15996-15997 . denotes .
T10566 15997-16170 sentence denotes Along the z-axis, the perinuclear distribution of AQP2-F204V was clearly seen, and this distribution is not altered by forskolin (Figure 4B, bottom row, two right columns).
T10567 15998-16003 IN denotes Along
T10569 16004-16007 DT denotes the
T10571 16008-16009 NN denotes z
T10572 16009-16010 HYPH denotes -
T10570 16010-16014 NN denotes axis
T10573 16014-16016 , denotes ,
T10574 16016-16019 DT denotes the
T10576 16020-16031 JJ denotes perinuclear
T10575 16032-16044 NN denotes distribution
T10577 16045-16047 IN denotes of
T10578 16048-16052 NN denotes AQP2
T10580 16052-16053 HYPH denotes -
T10579 16053-16058 NN denotes F204V
T10581 16059-16062 VBD denotes was
T10582 16063-16070 RB denotes clearly
T10568 16071-16075 VBN denotes seen
T10583 16075-16077 , denotes ,
T10584 16077-16080 CC denotes and
T10585 16081-16085 DT denotes this
T10586 16086-16098 NN denotes distribution
T10588 16099-16101 VBZ denotes is
T10589 16102-16105 RB denotes not
T10587 16106-16113 VBN denotes altered
T10590 16114-16116 IN denotes by
T10591 16117-16126 NN denotes forskolin
T10592 16127-16128 -LRB- denotes (
T10594 16128-16134 NN denotes Figure
T10595 16135-16137 CD denotes 4B
T10596 16137-16139 , denotes ,
T10597 16139-16145 JJ denotes bottom
T10598 16146-16149 NN denotes row
T10599 16149-16151 , denotes ,
T10600 16151-16154 CD denotes two
T10601 16155-16160 JJ denotes right
T10593 16161-16168 NNS denotes columns
T10602 16168-16169 -RRB- denotes )
T10603 16169-16170 . denotes .
T10604 16170-16260 sentence denotes The perinuclear distribution of AQP2-F204V is consistent with an ER compartmentalization.
T10605 16171-16174 DT denotes The
T10607 16175-16186 JJ denotes perinuclear
T10606 16187-16199 NN denotes distribution
T10609 16200-16202 IN denotes of
T10610 16203-16207 NN denotes AQP2
T10612 16207-16208 HYPH denotes -
T10611 16208-16213 NN denotes F204V
T10608 16214-16216 VBZ denotes is
T10613 16217-16227 JJ denotes consistent
T10614 16228-16232 IN denotes with
T10615 16233-16235 DT denotes an
T10617 16236-16238 NN denotes ER
T10616 16239-16259 NN denotes compartmentalization
T10618 16259-16260 . denotes .
T10619 16260-16417 sentence denotes To test the idea that AQP2-F204V localizes to the ER, we co-stained cells transfected with Aqp2F204V (cDNA) for AQP2 and an ER marker, calnexin (Figure 4C).
T10620 16261-16263 TO denotes To
T10621 16264-16268 VB denotes test
T10623 16269-16272 DT denotes the
T10624 16273-16277 NN denotes idea
T10625 16278-16282 IN denotes that
T10627 16283-16287 NN denotes AQP2
T10629 16287-16288 HYPH denotes -
T10628 16288-16293 NN denotes F204V
T10626 16294-16303 VBZ denotes localizes
T10630 16304-16306 IN denotes to
T10631 16307-16310 DT denotes the
T10632 16311-16313 NN denotes ER
T10633 16313-16315 , denotes ,
T10634 16315-16317 PRP denotes we
T10622 16318-16328 VBD denotes co-stained
T10635 16329-16334 NNS denotes cells
T10636 16335-16346 VBN denotes transfected
T10637 16347-16351 IN denotes with
T10638 16352-16361 NN denotes Aqp2F204V
T10639 16362-16363 -LRB- denotes (
T10640 16363-16367 NN denotes cDNA
T10641 16367-16368 -RRB- denotes )
T10642 16369-16372 IN denotes for
T10643 16373-16377 NN denotes AQP2
T10644 16378-16381 CC denotes and
T10645 16382-16384 DT denotes an
T10647 16385-16387 NN denotes ER
T10646 16388-16394 NN denotes marker
T10648 16394-16396 , denotes ,
T10649 16396-16404 NN denotes calnexin
T10650 16405-16406 -LRB- denotes (
T10651 16406-16412 NN denotes Figure
T10652 16413-16415 CD denotes 4C
T10653 16415-16416 -RRB- denotes )
T10654 16416-16417 . denotes .
T10655 16417-16560 sentence denotes Colocalization of calnexin with AQP2 was investigated directly, and it was found that 80% of all AQP2-F204V protein colocalized with calnexin.
T10656 16418-16432 NN denotes Colocalization
T10658 16433-16435 IN denotes of
T10659 16436-16444 NN denotes calnexin
T10660 16445-16449 IN denotes with
T10661 16450-16454 NN denotes AQP2
T10662 16455-16458 VBD denotes was
T10657 16459-16471 VBN denotes investigated
T10663 16472-16480 RB denotes directly
T10664 16480-16482 , denotes ,
T10665 16482-16485 CC denotes and
T10666 16486-16488 PRP denotes it
T10668 16489-16492 VBD denotes was
T10667 16493-16498 VBN denotes found
T10669 16499-16503 IN denotes that
T10671 16504-16506 CD denotes 80
T10672 16506-16507 NN denotes %
T10673 16508-16510 IN denotes of
T10674 16511-16514 DT denotes all
T10676 16515-16519 NN denotes AQP2
T10678 16519-16520 HYPH denotes -
T10677 16520-16525 NN denotes F204V
T10675 16526-16533 NN denotes protein
T10670 16534-16545 VBD denotes colocalized
T10679 16546-16550 IN denotes with
T10680 16551-16559 NN denotes calnexin
T10681 16559-16560 . denotes .
T10682 16560-16686 sentence denotes The remaining 20% appeared at the periphery of the ER, representing AQP2-F204V that had potentially progressed beyond the ER.
T10683 16561-16564 DT denotes The
T10685 16565-16574 VBG denotes remaining
T10686 16575-16577 CD denotes 20
T10684 16577-16578 NN denotes %
T10687 16579-16587 VBD denotes appeared
T10688 16588-16590 IN denotes at
T10689 16591-16594 DT denotes the
T10690 16595-16604 NN denotes periphery
T10691 16605-16607 IN denotes of
T10692 16608-16611 DT denotes the
T10693 16612-16614 NN denotes ER
T10694 16614-16616 , denotes ,
T10695 16616-16628 VBG denotes representing
T10696 16629-16633 NN denotes AQP2
T10698 16633-16634 HYPH denotes -
T10697 16634-16639 NN denotes F204V
T10699 16640-16644 WDT denotes that
T10701 16645-16648 VBD denotes had
T10702 16649-16660 RB denotes potentially
T10700 16661-16671 VBN denotes progressed
T10703 16672-16678 IN denotes beyond
T10704 16679-16682 DT denotes the
T10705 16683-16685 NN denotes ER
T10706 16685-16686 . denotes .
T10707 16686-16823 sentence denotes This “ER escape” was consistent with the small proportion of mature, complex glycosylated, AQP2-F204V in mutant kidneys (see Figure 3A).
T10708 16687-16691 DT denotes This
T10710 16692-16693 `` denotes “
T10711 16693-16695 NN denotes ER
T10709 16696-16702 NN denotes escape
T10713 16702-16703 '' denotes ”
T10712 16704-16707 VBD denotes was
T10714 16708-16718 JJ denotes consistent
T10715 16719-16723 IN denotes with
T10716 16724-16727 DT denotes the
T10718 16728-16733 JJ denotes small
T10717 16734-16744 NN denotes proportion
T10719 16745-16747 IN denotes of
T10720 16748-16754 JJ denotes mature
T10722 16754-16756 , denotes ,
T10723 16756-16763 JJ denotes complex
T10724 16764-16776 VBN denotes glycosylated
T10725 16776-16778 , denotes ,
T10726 16778-16782 NN denotes AQP2
T10727 16782-16783 HYPH denotes -
T10721 16783-16788 NN denotes F204V
T10728 16789-16791 IN denotes in
T10729 16792-16798 NN denotes mutant
T10730 16799-16806 NNS denotes kidneys
T10731 16807-16808 -LRB- denotes (
T10732 16808-16811 VB denotes see
T10733 16812-16818 NN denotes Figure
T10734 16819-16821 CD denotes 3A
T10735 16821-16822 -RRB- denotes )
T10736 16822-16823 . denotes .
T10737 16823-16959 sentence denotes Animals heterozygous for the Aqp2F204V mutation were not affected in their urine production or urine osmolality (see Figure 1C and 1D).
T10738 16824-16831 NNS denotes Animals
T10740 16832-16844 JJ denotes heterozygous
T10741 16845-16848 IN denotes for
T10742 16849-16852 DT denotes the
T10744 16853-16862 NN denotes Aqp2F204V
T10743 16863-16871 NN denotes mutation
T10745 16872-16876 VBD denotes were
T10746 16877-16880 RB denotes not
T10739 16881-16889 VBN denotes affected
T10747 16890-16892 IN denotes in
T10748 16893-16898 PRP$ denotes their
T10750 16899-16904 NN denotes urine
T10749 16905-16915 NN denotes production
T10751 16916-16918 CC denotes or
T10752 16919-16924 NN denotes urine
T10753 16925-16935 NN denotes osmolality
T10754 16936-16937 -LRB- denotes (
T10755 16937-16940 VB denotes see
T10756 16941-16947 NN denotes Figure
T10757 16948-16950 CD denotes 1C
T10758 16951-16954 CC denotes and
T10759 16955-16957 CD denotes 1D
T10760 16957-16958 -RRB- denotes )
T10761 16958-16959 . denotes .
T10762 16959-17130 sentence denotes It has also been shown that a recessive NDI allele, AQP2-R187C, does not interact with wild-type protein in oocytes [24], nor does it homo-oligomerize in MDCK cells [22].
T10763 16960-16962 PRP denotes It
T10765 16963-16966 VBZ denotes has
T10766 16967-16971 RB denotes also
T10767 16972-16976 VBN denotes been
T10764 16977-16982 VBN denotes shown
T10768 16983-16987 IN denotes that
T10770 16988-16989 DT denotes a
T10772 16990-16999 JJ denotes recessive
T10773 17000-17003 NN denotes NDI
T10771 17004-17010 NN denotes allele
T10774 17010-17012 , denotes ,
T10775 17012-17016 NN denotes AQP2
T10777 17016-17017 HYPH denotes -
T10776 17017-17022 NN denotes R187C
T10778 17022-17024 , denotes ,
T10779 17024-17028 VBZ denotes does
T10780 17029-17032 RB denotes not
T10769 17033-17041 VB denotes interact
T10781 17042-17046 IN denotes with
T10782 17047-17051 JJ denotes wild
T10784 17051-17052 HYPH denotes -
T10783 17052-17056 NN denotes type
T10785 17057-17064 NN denotes protein
T10786 17065-17067 IN denotes in
T10787 17068-17075 NNS denotes oocytes
T10788 17076-17077 -LRB- denotes [
T10789 17077-17079 CD denotes 24
T10790 17079-17080 -RRB- denotes ]
T10791 17080-17082 , denotes ,
T10792 17082-17085 CC denotes nor
T10793 17086-17090 VBZ denotes does
T10795 17091-17093 PRP denotes it
T10794 17094-17110 VB denotes homo-oligomerize
T10796 17111-17113 IN denotes in
T10797 17114-17118 NN denotes MDCK
T10798 17119-17124 NNS denotes cells
T10799 17125-17126 -LRB- denotes [
T10800 17126-17128 CD denotes 22
T10801 17128-17129 -RRB- denotes ]
T10802 17129-17130 . denotes .
T10803 17130-17238 sentence denotes Therefore, kidneys from heterozygous animals were examined for evidence of two populations of AQP2 protein.
T10804 17131-17140 RB denotes Therefore
T10806 17140-17142 , denotes ,
T10807 17142-17149 NNS denotes kidneys
T10808 17150-17154 IN denotes from
T10809 17155-17167 JJ denotes heterozygous
T10810 17168-17175 NNS denotes animals
T10811 17176-17180 VBD denotes were
T10805 17181-17189 VBN denotes examined
T10812 17190-17193 IN denotes for
T10813 17194-17202 NN denotes evidence
T10814 17203-17205 IN denotes of
T10815 17206-17209 CD denotes two
T10816 17210-17221 NNS denotes populations
T10817 17222-17224 IN denotes of
T10818 17225-17229 NN denotes AQP2
T10819 17230-17237 NN denotes protein
T10820 17237-17238 . denotes .
T10821 17238-17394 sentence denotes Surprisingly, immunohistochemical staining of kidney collecting ducts from Aqp2F204V/+ mice revealed a pattern remarkably similar to wild type (Figure 5A).
T10822 17239-17251 RB denotes Surprisingly
T10824 17251-17253 , denotes ,
T10825 17253-17272 JJ denotes immunohistochemical
T10826 17273-17281 NN denotes staining
T10827 17282-17284 IN denotes of
T10828 17285-17291 NN denotes kidney
T10830 17292-17302 VBG denotes collecting
T10829 17303-17308 NNS denotes ducts
T10831 17309-17313 IN denotes from
T10832 17314-17323 NN denotes Aqp2F204V
T10834 17323-17324 HYPH denotes /
T10835 17324-17325 SYM denotes +
T10833 17326-17330 NNS denotes mice
T10823 17331-17339 VBD denotes revealed
T10836 17340-17341 DT denotes a
T10837 17342-17349 NN denotes pattern
T10838 17350-17360 RB denotes remarkably
T10839 17361-17368 JJ denotes similar
T10840 17369-17371 IN denotes to
T10841 17372-17376 JJ denotes wild
T10842 17377-17381 NN denotes type
T10843 17382-17383 -LRB- denotes (
T10844 17383-17389 NN denotes Figure
T10845 17390-17392 CD denotes 5A
T10846 17392-17393 -RRB- denotes )
T10847 17393-17394 . denotes .
T10848 17394-17474 sentence denotes AQP2 translocated completely to the apical cell surface upon dDAVP stimulation.
T10849 17395-17399 NN denotes AQP2
T10850 17400-17412 VBD denotes translocated
T10851 17413-17423 RB denotes completely
T10852 17424-17426 IN denotes to
T10853 17427-17430 DT denotes the
T10855 17431-17437 JJ denotes apical
T10856 17438-17442 NN denotes cell
T10854 17443-17450 NN denotes surface
T10857 17451-17455 IN denotes upon
T10858 17456-17461 NN denotes dDAVP
T10859 17462-17473 NN denotes stimulation
T10860 17473-17474 . denotes .
T10861 17474-17633 sentence denotes This wild-type staining pattern may simply reflect the fact that decreasing the amount of mutant protein by half makes it undetectable by immunocytochemistry.
T10862 17475-17479 DT denotes This
T10864 17480-17484 JJ denotes wild
T10866 17484-17485 HYPH denotes -
T10865 17485-17489 NN denotes type
T10867 17490-17498 NN denotes staining
T10863 17499-17506 NN denotes pattern
T10869 17507-17510 MD denotes may
T10870 17511-17517 RB denotes simply
T10868 17518-17525 VB denotes reflect
T10871 17526-17529 DT denotes the
T10872 17530-17534 NN denotes fact
T10873 17535-17539 IN denotes that
T10875 17540-17550 VBG denotes decreasing
T10876 17551-17554 DT denotes the
T10877 17555-17561 NN denotes amount
T10878 17562-17564 IN denotes of
T10879 17565-17571 NN denotes mutant
T10880 17572-17579 NN denotes protein
T10881 17580-17582 IN denotes by
T10882 17583-17587 NN denotes half
T10874 17588-17593 VBZ denotes makes
T10883 17594-17596 PRP denotes it
T10884 17597-17609 JJ denotes undetectable
T10885 17610-17612 IN denotes by
T10886 17613-17632 NN denotes immunocytochemistry
T10887 17632-17633 . denotes .
T10888 17633-17732 sentence denotes Alternatively, the presence of wild-type protein may alter the localization of the mutant protein.
T10889 17634-17647 RB denotes Alternatively
T10891 17647-17649 , denotes ,
T10892 17649-17652 DT denotes the
T10893 17653-17661 NN denotes presence
T10894 17662-17664 IN denotes of
T10895 17665-17669 JJ denotes wild
T10897 17669-17670 HYPH denotes -
T10896 17670-17674 NN denotes type
T10898 17675-17682 NN denotes protein
T10899 17683-17686 MD denotes may
T10890 17687-17692 VB denotes alter
T10900 17693-17696 DT denotes the
T10901 17697-17709 NN denotes localization
T10902 17710-17712 IN denotes of
T10903 17713-17716 DT denotes the
T10905 17717-17723 NN denotes mutant
T10904 17724-17731 NN denotes protein
T10906 17731-17732 . denotes .
T10907 17732-17902 sentence denotes Indeed, Hendriks et al. proposed a “piggy-back” mechanism to explain the transport of nonglycosylated subunits of AQP2 to the cell surface by glycosylated subunits [22].
T10908 17733-17739 RB denotes Indeed
T10910 17739-17741 , denotes ,
T10911 17741-17749 NNP denotes Hendriks
T10912 17750-17752 FW denotes et
T10913 17753-17756 FW denotes al.
T10909 17757-17765 VBD denotes proposed
T10914 17766-17767 DT denotes a
T10916 17768-17769 `` denotes “
T10917 17769-17774 NN denotes piggy
T10919 17774-17775 HYPH denotes -
T10918 17775-17779 NN denotes back
T10920 17779-17780 '' denotes ”
T10915 17781-17790 NN denotes mechanism
T10921 17791-17793 TO denotes to
T10922 17794-17801 VB denotes explain
T10923 17802-17805 DT denotes the
T10924 17806-17815 NN denotes transport
T10925 17816-17818 IN denotes of
T10926 17819-17834 JJ denotes nonglycosylated
T10927 17835-17843 NNS denotes subunits
T10928 17844-17846 IN denotes of
T10929 17847-17851 NN denotes AQP2
T10930 17852-17854 IN denotes to
T10931 17855-17858 DT denotes the
T10933 17859-17863 NN denotes cell
T10932 17864-17871 NN denotes surface
T10934 17872-17874 IN denotes by
T10935 17875-17887 VBN denotes glycosylated
T10936 17888-17896 NNS denotes subunits
T10937 17897-17898 -LRB- denotes [
T10938 17898-17900 CD denotes 22
T10939 17900-17901 -RRB- denotes ]
T10940 17901-17902 . denotes .
T10941 17902-18067 sentence denotes It has also been shown that wild-type AQP2 protein can rescue a translocation-defective mutant protein, AQP2-P262L, when the two are coexpressed in MDCK cells [25].
T10942 17903-17905 PRP denotes It
T10944 17906-17909 VBZ denotes has
T10945 17910-17914 RB denotes also
T10946 17915-17919 VBN denotes been
T10943 17920-17925 VBN denotes shown
T10947 17926-17930 IN denotes that
T10949 17931-17935 JJ denotes wild
T10951 17935-17936 HYPH denotes -
T10950 17936-17940 NN denotes type
T10953 17941-17945 NN denotes AQP2
T10952 17946-17953 NN denotes protein
T10954 17954-17957 MD denotes can
T10948 17958-17964 VB denotes rescue
T10955 17965-17966 DT denotes a
T10957 17967-17980 NN denotes translocation
T10959 17980-17981 HYPH denotes -
T10958 17981-17990 JJ denotes defective
T10960 17991-17997 NN denotes mutant
T10956 17998-18005 NN denotes protein
T10961 18005-18007 , denotes ,
T10962 18007-18011 NN denotes AQP2
T10964 18011-18012 HYPH denotes -
T10963 18012-18017 NN denotes P262L
T10965 18017-18019 , denotes ,
T10966 18019-18023 WRB denotes when
T10968 18024-18027 DT denotes the
T10969 18028-18031 CD denotes two
T10970 18032-18035 VBP denotes are
T10967 18036-18047 VBN denotes coexpressed
T10971 18048-18050 IN denotes in
T10972 18051-18055 NN denotes MDCK
T10973 18056-18061 NNS denotes cells
T10974 18062-18063 -LRB- denotes [
T10975 18063-18065 CD denotes 25
T10976 18065-18066 -RRB- denotes ]
T10977 18066-18067 . denotes .
T10978 18067-19830 sentence denotes Figure 5 AQP2-F204V Rescue in Heterozygous Mouse Collecting Ducts and in Cotransfected MDCK Cells (A) In heterozygous animals, AQP2 localizes and responds to dDAVP normally. Immunohistochemistry was carried out on kidney sections from an Aqp2F204V/+ mouse, after injection with dDAVP. Kidney sections were sequentially immunostained for AQP2 (red) and the basolateral marker AQP3 (green). (B) Mutant and wild-type AQP2 physically interact. MDCK cells stably expressing wild-type AQP2 were transiently transfected with GFP tagged wild-type AQP2, AQP2-F204V, or GFP alone. Solubilized membranes were immunoprecipitated with a GFP antibody. Total membranes and immunoprecipitates (GFP-IP) were Western blotted using an antibody against AQP2 (arrow) or AQP2-GFP fusions (arrowhead). (C) Wild-type AQP2 rescues the localization defect of mutant AQP2. GFP fusions of either wild-type AQP2 (WT-GFP, top photomicrographs) or F204V AQP2 (F204V-GFP, bottom photomicrographs) were expressed in polarized MDCK stable cell lines expressing vector alone (vector, left photomicrographs) or AQP2-WT (right photomicrographs). Cells were stimulated with forskolin, processed for immunocytochemistry, and used to generate z-sectional images. (D) Mutant AQP2 is present at the cell surface in cells coexpressing wild-type AQP2 (AQP2-WT). GFP fused to AQP2-F204V was expressed in MDCK cells expressing wild-type AQP2 or vector alone. Cells were stimulated with forskolin, and cell surface biotinylated proteins were precipitated then analyzed for the presence of wild-type AQP2 (arrow) and AQP2-F204V (arrowhead) by Western blot. In the collecting ducts from Aqp2F204V/+ mice, the wild type may rescue the mutant protein as suggested by the subcellular distribution of AQP2 protein.
T10979 19678-19680 IN denotes In
T10981 19681-19684 DT denotes the
T10983 19685-19695 VBG denotes collecting
T10982 19696-19701 NNS denotes ducts
T10984 19702-19706 IN denotes from
T10985 19707-19716 NN denotes Aqp2F204V
T10987 19716-19717 HYPH denotes /
T10988 19717-19718 SYM denotes +
T10986 19719-19723 NNS denotes mice
T10989 19723-19725 , denotes ,
T10990 19725-19728 DT denotes the
T10992 19729-19733 JJ denotes wild
T10991 19734-19738 NN denotes type
T10993 19739-19742 MD denotes may
T10980 19743-19749 VB denotes rescue
T10994 19750-19753 DT denotes the
T10996 19754-19760 NN denotes mutant
T10995 19761-19768 NN denotes protein
T10997 19769-19771 IN denotes as
T10998 19772-19781 VBN denotes suggested
T10999 19782-19784 IN denotes by
T11000 19785-19788 DT denotes the
T11002 19789-19800 JJ denotes subcellular
T11001 19801-19813 NN denotes distribution
T11003 19814-19816 IN denotes of
T11004 19817-19821 NN denotes AQP2
T11005 19822-19829 NN denotes protein
T11006 19829-19830 . denotes .
T11007 19830-19961 sentence denotes To test this idea, we first looked for an interaction between mutant and wild-type proteins in transfected MDCK cells (Figure 5B).
T11008 19831-19833 TO denotes To
T11009 19834-19838 VB denotes test
T11011 19839-19843 DT denotes this
T11012 19844-19848 NN denotes idea
T11013 19848-19850 , denotes ,
T11014 19850-19852 PRP denotes we
T11015 19853-19858 RB denotes first
T11010 19859-19865 VBD denotes looked
T11016 19866-19869 IN denotes for
T11017 19870-19872 DT denotes an
T11018 19873-19884 NN denotes interaction
T11019 19885-19892 IN denotes between
T11020 19893-19899 NN denotes mutant
T11022 19900-19903 CC denotes and
T11023 19904-19908 JJ denotes wild
T11025 19908-19909 HYPH denotes -
T11024 19909-19913 NN denotes type
T11021 19914-19922 NN denotes proteins
T11026 19923-19925 IN denotes in
T11027 19926-19937 VBN denotes transfected
T11029 19938-19942 NN denotes MDCK
T11028 19943-19948 NNS denotes cells
T11030 19949-19950 -LRB- denotes (
T11031 19950-19956 NN denotes Figure
T11032 19957-19959 CD denotes 5B
T11033 19959-19960 -RRB- denotes )
T11034 19960-19961 . denotes .
T11035 19961-20127 sentence denotes MDCK cells stably expressing wild-type AQP2 were transiently transfected with GFP expression constructs encoding GFP-tagged wild-type AQP2, AQP2-F204V, or GFP alone.
T11036 19962-19966 NN denotes MDCK
T11037 19967-19972 NNS denotes cells
T11039 19973-19979 RB denotes stably
T11040 19980-19990 VBG denotes expressing
T11041 19991-19995 JJ denotes wild
T11043 19995-19996 HYPH denotes -
T11042 19996-20000 NN denotes type
T11044 20001-20005 NN denotes AQP2
T11045 20006-20010 VBD denotes were
T11046 20011-20022 RB denotes transiently
T11038 20023-20034 VBN denotes transfected
T11047 20035-20039 IN denotes with
T11048 20040-20043 NN denotes GFP
T11050 20044-20054 NN denotes expression
T11049 20055-20065 NNS denotes constructs
T11051 20066-20074 VBG denotes encoding
T11052 20075-20078 NN denotes GFP
T11054 20078-20079 HYPH denotes -
T11053 20079-20085 VBN denotes tagged
T11056 20086-20090 JJ denotes wild
T11058 20090-20091 HYPH denotes -
T11057 20091-20095 NN denotes type
T11055 20096-20100 NN denotes AQP2
T11059 20100-20102 , denotes ,
T11060 20102-20106 NN denotes AQP2
T11062 20106-20107 HYPH denotes -
T11061 20107-20112 NN denotes F204V
T11063 20112-20114 , denotes ,
T11064 20114-20116 CC denotes or
T11065 20117-20120 NN denotes GFP
T11066 20121-20126 RB denotes alone
T11067 20126-20127 . denotes .
T11068 20127-20377 sentence denotes Antibodies against GFP coimmunoprecipitated wild-type AQP2 when AQP2-GFP or AQP2-F204V-GFP was transiently transfected, but not when GFP by itself was transiently transfected into MDCK cells stably expressing wild-type AQP2 (Figure 5B, upper blots).
T11069 20128-20138 NNS denotes Antibodies
T11071 20139-20146 IN denotes against
T11072 20147-20150 NN denotes GFP
T11070 20151-20171 VBD denotes coimmunoprecipitated
T11073 20172-20176 JJ denotes wild
T11075 20176-20177 HYPH denotes -
T11074 20177-20181 NN denotes type
T11076 20182-20186 NN denotes AQP2
T11077 20187-20191 WRB denotes when
T11079 20192-20196 NN denotes AQP2
T11081 20196-20197 HYPH denotes -
T11080 20197-20200 NN denotes GFP
T11082 20201-20203 CC denotes or
T11083 20204-20208 NN denotes AQP2
T11085 20208-20209 HYPH denotes -
T11086 20209-20214 NN denotes F204V
T11087 20214-20215 HYPH denotes -
T11084 20215-20218 NN denotes GFP
T11088 20219-20222 VBD denotes was
T11089 20223-20234 RB denotes transiently
T11078 20235-20246 VBN denotes transfected
T11090 20246-20248 , denotes ,
T11091 20248-20251 CC denotes but
T11092 20252-20255 RB denotes not
T11093 20256-20260 WRB denotes when
T11095 20261-20264 NN denotes GFP
T11096 20265-20267 IN denotes by
T11097 20268-20274 PRP denotes itself
T11098 20275-20278 VBD denotes was
T11099 20279-20290 RB denotes transiently
T11094 20291-20302 VBN denotes transfected
T11100 20303-20307 IN denotes into
T11101 20308-20312 NN denotes MDCK
T11102 20313-20318 NNS denotes cells
T11103 20319-20325 RB denotes stably
T11104 20326-20336 VBG denotes expressing
T11105 20337-20341 JJ denotes wild
T11107 20341-20342 HYPH denotes -
T11106 20342-20346 NN denotes type
T11108 20347-20351 NN denotes AQP2
T11109 20352-20353 -LRB- denotes (
T11111 20353-20359 NN denotes Figure
T11112 20360-20362 CD denotes 5B
T11113 20362-20364 , denotes ,
T11114 20364-20369 JJ denotes upper
T11110 20370-20375 NNS denotes blots
T11115 20375-20376 -RRB- denotes )
T11116 20376-20377 . denotes .
T11117 20377-20507 sentence denotes Western blot of total membranes showed that wild-type AQP2 is equivalently expressed in all three cases (Figure 5B, lower blots).
T11118 20378-20385 NNP denotes Western
T11119 20386-20390 NN denotes blot
T11121 20391-20393 IN denotes of
T11122 20394-20399 JJ denotes total
T11123 20400-20409 NNS denotes membranes
T11120 20410-20416 VBD denotes showed
T11124 20417-20421 IN denotes that
T11126 20422-20426 JJ denotes wild
T11128 20426-20427 HYPH denotes -
T11127 20427-20431 NN denotes type
T11129 20432-20436 NN denotes AQP2
T11130 20437-20439 VBZ denotes is
T11131 20440-20452 RB denotes equivalently
T11125 20453-20462 VBN denotes expressed
T11132 20463-20465 IN denotes in
T11133 20466-20469 DT denotes all
T11135 20470-20475 CD denotes three
T11134 20476-20481 NNS denotes cases
T11136 20482-20483 -LRB- denotes (
T11138 20483-20489 NN denotes Figure
T11139 20490-20492 CD denotes 5B
T11140 20492-20494 , denotes ,
T11141 20494-20499 JJ denotes lower
T11137 20500-20505 NNS denotes blots
T11142 20505-20506 -RRB- denotes )
T11143 20506-20507 . denotes .
T11144 20507-20653 sentence denotes If wild-type and mutant proteins are indeed interacting in the cell, is this interaction sufficient to rescue the localization of mutant protein?
T11145 20508-20510 IN denotes If
T11147 20511-20515 JJ denotes wild
T11149 20515-20516 HYPH denotes -
T11148 20516-20520 NN denotes type
T11151 20521-20524 CC denotes and
T11152 20525-20531 NN denotes mutant
T11150 20532-20540 NN denotes proteins
T11153 20541-20544 VBP denotes are
T11154 20545-20551 RB denotes indeed
T11146 20552-20563 VBG denotes interacting
T11156 20564-20566 IN denotes in
T11157 20567-20570 DT denotes the
T11158 20571-20575 NN denotes cell
T11159 20575-20577 , denotes ,
T11155 20577-20579 VBZ denotes is
T11160 20580-20584 DT denotes this
T11161 20585-20596 NN denotes interaction
T11162 20597-20607 JJ denotes sufficient
T11163 20608-20610 TO denotes to
T11164 20611-20617 VB denotes rescue
T11165 20618-20621 DT denotes the
T11166 20622-20634 NN denotes localization
T11167 20635-20637 IN denotes of
T11168 20638-20644 NN denotes mutant
T11169 20645-20652 NN denotes protein
T11170 20652-20653 . denotes ?
T11171 20653-20776 sentence denotes To answer this question, we used MDCK cells stably transfected with wild-type AQP2 expressing vector or with empty vector.
T11172 20654-20656 TO denotes To
T11173 20657-20663 VB denotes answer
T11175 20664-20668 DT denotes this
T11176 20669-20677 NN denotes question
T11177 20677-20679 , denotes ,
T11178 20679-20681 PRP denotes we
T11174 20682-20686 VBD denotes used
T11179 20687-20691 NN denotes MDCK
T11180 20692-20697 NNS denotes cells
T11181 20698-20704 RB denotes stably
T11182 20705-20716 VBN denotes transfected
T11183 20717-20721 IN denotes with
T11184 20722-20726 JJ denotes wild
T11186 20726-20727 HYPH denotes -
T11185 20727-20731 NN denotes type
T11187 20732-20736 NN denotes AQP2
T11188 20737-20747 VBG denotes expressing
T11189 20748-20754 NN denotes vector
T11190 20755-20757 CC denotes or
T11191 20758-20762 IN denotes with
T11192 20763-20768 JJ denotes empty
T11193 20769-20775 NN denotes vector
T11194 20775-20776 . denotes .
T11195 20776-20870 sentence denotes On top of these, we transiently transfected AQP2-GFP or AQP2-F204V-GFP expression constructs.
T11196 20777-20779 IN denotes On
T11198 20780-20783 NN denotes top
T11199 20784-20786 IN denotes of
T11200 20787-20792 DT denotes these
T11201 20792-20794 , denotes ,
T11202 20794-20796 PRP denotes we
T11203 20797-20808 RB denotes transiently
T11197 20809-20820 VBD denotes transfected
T11204 20821-20825 NN denotes AQP2
T11206 20825-20826 HYPH denotes -
T11205 20826-20829 NN denotes GFP
T11208 20830-20832 CC denotes or
T11209 20833-20837 NN denotes AQP2
T11211 20837-20838 HYPH denotes -
T11212 20838-20843 NN denotes F204V
T11213 20843-20844 HYPH denotes -
T11210 20844-20847 NN denotes GFP
T11214 20848-20858 NN denotes expression
T11207 20859-20869 NNS denotes constructs
T11215 20869-20870 . denotes .
T11216 20870-21086 sentence denotes AQP2-GFP localized to the apical surface upon forskolin stimulation whether it was transiently transfected into vector-only cells (Figure 5C, upper left images) or into wild-type AQP2 cells (Figure 5C, upper right).
T11217 20871-20875 NN denotes AQP2
T11219 20875-20876 HYPH denotes -
T11218 20876-20879 NN denotes GFP
T11220 20880-20889 VBD denotes localized
T11221 20890-20892 IN denotes to
T11222 20893-20896 DT denotes the
T11224 20897-20903 JJ denotes apical
T11223 20904-20911 NN denotes surface
T11225 20912-20916 IN denotes upon
T11226 20917-20926 NN denotes forskolin
T11227 20927-20938 NN denotes stimulation
T11228 20939-20946 IN denotes whether
T11230 20947-20949 PRP denotes it
T11231 20950-20953 VBD denotes was
T11232 20954-20965 RB denotes transiently
T11229 20966-20977 VBN denotes transfected
T11233 20978-20982 IN denotes into
T11234 20983-20989 NN denotes vector
T11236 20989-20990 HYPH denotes -
T11235 20990-20994 JJ denotes only
T11237 20995-21000 NNS denotes cells
T11238 21001-21002 -LRB- denotes (
T11240 21002-21008 NN denotes Figure
T11241 21009-21011 CD denotes 5C
T11242 21011-21013 , denotes ,
T11243 21013-21018 JJ denotes upper
T11244 21019-21023 JJ denotes left
T11239 21024-21030 NNS denotes images
T11245 21030-21031 -RRB- denotes )
T11246 21032-21034 CC denotes or
T11247 21035-21039 IN denotes into
T11248 21040-21044 JJ denotes wild
T11250 21044-21045 HYPH denotes -
T11249 21045-21049 NN denotes type
T11252 21050-21054 NN denotes AQP2
T11251 21055-21060 NNS denotes cells
T11253 21061-21062 -LRB- denotes (
T11254 21062-21068 NN denotes Figure
T11255 21069-21071 CD denotes 5C
T11256 21071-21073 , denotes ,
T11257 21073-21078 JJ denotes upper
T11258 21079-21084 JJ denotes right
T11259 21084-21085 -RRB- denotes )
T11260 21085-21086 . denotes .
T11261 21086-21242 sentence denotes AQP2-F204V-GFP, when expressed by transient transfection into vector only cells, showed a diffuse cytoplasmic distribution pattern (Figure 5C, lower left).
T11262 21087-21091 NN denotes AQP2
T11264 21091-21092 HYPH denotes -
T11265 21092-21097 NN denotes F204V
T11266 21097-21098 HYPH denotes -
T11263 21098-21101 NN denotes GFP
T11268 21101-21103 , denotes ,
T11269 21103-21107 WRB denotes when
T11270 21108-21117 VBN denotes expressed
T11271 21118-21120 IN denotes by
T11272 21121-21130 JJ denotes transient
T11273 21131-21143 NN denotes transfection
T11274 21144-21148 IN denotes into
T11275 21149-21155 NN denotes vector
T11276 21156-21160 JJ denotes only
T11277 21161-21166 NNS denotes cells
T11278 21166-21168 , denotes ,
T11267 21168-21174 VBD denotes showed
T11279 21175-21176 DT denotes a
T11281 21177-21184 JJ denotes diffuse
T11282 21185-21196 JJ denotes cytoplasmic
T11283 21197-21209 NN denotes distribution
T11280 21210-21217 NN denotes pattern
T11284 21218-21219 -LRB- denotes (
T11286 21219-21225 NN denotes Figure
T11287 21226-21228 CD denotes 5C
T11288 21228-21230 , denotes ,
T11289 21230-21235 JJ denotes lower
T11285 21236-21240 JJ denotes left
T11290 21240-21241 -RRB- denotes )
T11291 21241-21242 . denotes .
T11292 21242-21398 sentence denotes When expressed in wild-type AQP2 cells, however, AQP2-F204V-GFP localized to the apical surface to varying degrees (Figure 5C, lower right images [i–iii]).
T11293 21243-21247 WRB denotes When
T11294 21248-21257 VBN denotes expressed
T11296 21258-21260 IN denotes in
T11297 21261-21265 JJ denotes wild
T11299 21265-21266 HYPH denotes -
T11298 21266-21270 NN denotes type
T11301 21271-21275 NN denotes AQP2
T11300 21276-21281 NNS denotes cells
T11302 21281-21283 , denotes ,
T11303 21283-21290 RB denotes however
T11304 21290-21292 , denotes ,
T11305 21292-21296 NN denotes AQP2
T11307 21296-21297 HYPH denotes -
T11308 21297-21302 NN denotes F204V
T11309 21302-21303 HYPH denotes -
T11306 21303-21306 NN denotes GFP
T11295 21307-21316 VBD denotes localized
T11310 21317-21319 IN denotes to
T11311 21320-21323 DT denotes the
T11313 21324-21330 JJ denotes apical
T11312 21331-21338 NN denotes surface
T11314 21339-21341 IN denotes to
T11315 21342-21349 VBG denotes varying
T11316 21350-21357 NNS denotes degrees
T11317 21358-21359 -LRB- denotes (
T11319 21359-21365 NN denotes Figure
T11320 21366-21368 CD denotes 5C
T11321 21368-21370 , denotes ,
T11322 21370-21375 JJ denotes lower
T11323 21376-21381 JJ denotes right
T11318 21382-21388 NNS denotes images
T11324 21389-21390 -LRB- denotes [
T11325 21390-21391 CD denotes i
T11326 21391-21392 SYM denotes –
T11327 21392-21395 CD denotes iii
T11328 21395-21396 -RRB- denotes ]
T11329 21396-21397 -RRB- denotes )
T11330 21397-21398 . denotes .
T11331 21398-21480 sentence denotes The lower right images of Figure 5C shows three cells from a single transfection.
T11332 21399-21402 DT denotes The
T11334 21403-21408 JJ denotes lower
T11335 21409-21414 JJ denotes right
T11333 21415-21421 NNS denotes images
T11337 21422-21424 IN denotes of
T11338 21425-21431 NN denotes Figure
T11339 21432-21434 CD denotes 5C
T11336 21435-21440 VBZ denotes shows
T11340 21441-21446 CD denotes three
T11341 21447-21452 NNS denotes cells
T11342 21453-21457 IN denotes from
T11343 21458-21459 DT denotes a
T11345 21460-21466 JJ denotes single
T11344 21467-21479 NN denotes transfection
T11346 21479-21480 . denotes .
T11347 21480-21628 sentence denotes The first is a nontransfected cell that shows the localization of the stably expressing wild-type AQP2, which is apical upon forskolin stimulation.
T11348 21481-21484 DT denotes The
T11349 21485-21490 JJ denotes first
T11350 21491-21493 VBZ denotes is
T11351 21494-21495 DT denotes a
T11353 21496-21510 JJ denotes nontransfected
T11352 21511-21515 NN denotes cell
T11354 21516-21520 WDT denotes that
T11355 21521-21526 VBZ denotes shows
T11356 21527-21530 DT denotes the
T11357 21531-21543 NN denotes localization
T11358 21544-21546 IN denotes of
T11359 21547-21550 DT denotes the
T11361 21551-21557 RB denotes stably
T11362 21558-21568 VBG denotes expressing
T11363 21569-21573 JJ denotes wild
T11365 21573-21574 HYPH denotes -
T11364 21574-21578 NN denotes type
T11360 21579-21583 NN denotes AQP2
T11366 21583-21585 , denotes ,
T11367 21585-21590 WDT denotes which
T11368 21591-21593 VBZ denotes is
T11369 21594-21600 JJ denotes apical
T11370 21601-21605 IN denotes upon
T11371 21606-21615 NN denotes forskolin
T11372 21616-21627 NN denotes stimulation
T11373 21627-21628 . denotes .
T11374 21628-21725 sentence denotes The next two show expression of both the stable wild-type AQP2 and the transient AQP2-F204V-GFP.
T11375 21629-21632 DT denotes The
T11377 21633-21637 JJ denotes next
T11376 21638-21641 CD denotes two
T11378 21642-21646 VBP denotes show
T11379 21647-21657 NN denotes expression
T11380 21658-21660 IN denotes of
T11381 21661-21665 CC denotes both
T11383 21666-21669 DT denotes the
T11384 21670-21676 JJ denotes stable
T11385 21677-21681 JJ denotes wild
T11387 21681-21682 HYPH denotes -
T11386 21682-21686 NN denotes type
T11382 21687-21691 NN denotes AQP2
T11388 21692-21695 CC denotes and
T11389 21696-21699 DT denotes the
T11391 21700-21709 JJ denotes transient
T11392 21710-21714 NN denotes AQP2
T11393 21714-21715 HYPH denotes -
T11394 21715-21720 NN denotes F204V
T11395 21720-21721 HYPH denotes -
T11390 21721-21724 NN denotes GFP
T11396 21724-21725 . denotes .
T11397 21725-21858 sentence denotes In cell (ii), localization of wild-type AQP2 was indistinguishable from AQP2-F204V-GFP; both were apical upon forskolin stimulation.
T11398 21726-21728 IN denotes In
T11400 21729-21733 NN denotes cell
T11401 21734-21735 -LRB- denotes (
T11402 21735-21737 CD denotes ii
T11403 21737-21738 -RRB- denotes )
T11404 21738-21740 , denotes ,
T11405 21740-21752 NN denotes localization
T11406 21753-21755 IN denotes of
T11407 21756-21760 JJ denotes wild
T11409 21760-21761 HYPH denotes -
T11408 21761-21765 NN denotes type
T11410 21766-21770 NN denotes AQP2
T11399 21771-21774 VBD denotes was
T11412 21775-21792 JJ denotes indistinguishable
T11413 21793-21797 IN denotes from
T11414 21798-21802 NN denotes AQP2
T11416 21802-21803 HYPH denotes -
T11417 21803-21808 NN denotes F204V
T11418 21808-21809 HYPH denotes -
T11415 21809-21812 NN denotes GFP
T11419 21812-21813 : denotes ;
T11420 21814-21818 DT denotes both
T11411 21819-21823 VBD denotes were
T11421 21824-21830 JJ denotes apical
T11422 21831-21835 IN denotes upon
T11423 21836-21845 NN denotes forskolin
T11424 21846-21857 NN denotes stimulation
T11425 21857-21858 . denotes .
T11426 21858-21963 sentence denotes Although the effect was subtle in cell (iii), AQP2-F204V-GFP was partly localized to the apical surface.
T11427 21859-21867 IN denotes Although
T11429 21868-21871 DT denotes the
T11430 21872-21878 NN denotes effect
T11428 21879-21882 VBD denotes was
T11432 21883-21889 JJ denotes subtle
T11433 21890-21892 IN denotes in
T11434 21893-21897 NN denotes cell
T11435 21898-21899 -LRB- denotes (
T11436 21899-21902 CD denotes iii
T11437 21902-21903 -RRB- denotes )
T11438 21903-21905 , denotes ,
T11439 21905-21909 NN denotes AQP2
T11441 21909-21910 HYPH denotes -
T11442 21910-21915 NN denotes F204V
T11443 21915-21916 HYPH denotes -
T11440 21916-21919 NN denotes GFP
T11444 21920-21923 VBD denotes was
T11445 21924-21930 RB denotes partly
T11431 21931-21940 VBN denotes localized
T11446 21941-21943 IN denotes to
T11447 21944-21947 DT denotes the
T11449 21948-21954 JJ denotes apical
T11448 21955-21962 NN denotes surface
T11450 21962-21963 . denotes .
T11451 21963-22073 sentence denotes Generally, the localization of AQP2-F204V-GFP was clearly more apical when wild-type AQP2 was also expressed.
T11452 21964-21973 RB denotes Generally
T11454 21973-21975 , denotes ,
T11455 21975-21978 DT denotes the
T11456 21979-21991 NN denotes localization
T11457 21992-21994 IN denotes of
T11458 21995-21999 NN denotes AQP2
T11460 21999-22000 HYPH denotes -
T11461 22000-22005 NN denotes F204V
T11462 22005-22006 HYPH denotes -
T11459 22006-22009 NN denotes GFP
T11453 22010-22013 VBD denotes was
T11463 22014-22021 RB denotes clearly
T11464 22022-22026 RBR denotes more
T11465 22027-22033 JJ denotes apical
T11466 22034-22038 WRB denotes when
T11468 22039-22043 JJ denotes wild
T11470 22043-22044 HYPH denotes -
T11469 22044-22048 NN denotes type
T11471 22049-22053 NN denotes AQP2
T11472 22054-22057 VBD denotes was
T11473 22058-22062 RB denotes also
T11467 22063-22072 VBN denotes expressed
T11474 22072-22073 . denotes .
T11475 22073-22306 sentence denotes To confirm these results biochemically, we transfected the same cell lines (wild-type AQP2 or vector) with F204V-GFP, biotinylated surface proteins after forskolin stimulation, and precipitated the biotinylated proteins (Figure 5D).
T11476 22074-22076 TO denotes To
T11477 22077-22084 VB denotes confirm
T11479 22085-22090 DT denotes these
T11480 22091-22098 NNS denotes results
T11481 22099-22112 RB denotes biochemically
T11482 22112-22114 , denotes ,
T11483 22114-22116 PRP denotes we
T11478 22117-22128 VBD denotes transfected
T11484 22129-22132 DT denotes the
T11486 22133-22137 JJ denotes same
T11487 22138-22142 NN denotes cell
T11485 22143-22148 NNS denotes lines
T11488 22149-22150 -LRB- denotes (
T11489 22150-22154 JJ denotes wild
T11491 22154-22155 HYPH denotes -
T11490 22155-22159 NN denotes type
T11492 22160-22164 NN denotes AQP2
T11493 22165-22167 CC denotes or
T11494 22168-22174 NN denotes vector
T11495 22174-22175 -RRB- denotes )
T11496 22176-22180 IN denotes with
T11497 22181-22186 NN denotes F204V
T11499 22186-22187 HYPH denotes -
T11498 22187-22190 NN denotes GFP
T11500 22190-22192 , denotes ,
T11501 22192-22204 VBD denotes biotinylated
T11502 22205-22212 NN denotes surface
T11503 22213-22221 NN denotes proteins
T11504 22222-22227 IN denotes after
T11505 22228-22237 NN denotes forskolin
T11506 22238-22249 NN denotes stimulation
T11507 22249-22251 , denotes ,
T11508 22251-22254 CC denotes and
T11509 22255-22267 VBD denotes precipitated
T11510 22268-22271 DT denotes the
T11512 22272-22284 VBN denotes biotinylated
T11511 22285-22293 NN denotes proteins
T11513 22294-22295 -LRB- denotes (
T11514 22295-22301 NN denotes Figure
T11515 22302-22304 CD denotes 5D
T11516 22304-22305 -RRB- denotes )
T11517 22305-22306 . denotes .
T11518 22306-22499 sentence denotes AQP2-F204V-GFP is expressed approximately equally in both cell lines (Figure 5D, total cells), but is biotinylated only when wild-type AQP2 is also expressed (Figure 5D, surface biotinylated).
T11519 22307-22311 NN denotes AQP2
T11521 22311-22312 HYPH denotes -
T11522 22312-22317 NN denotes F204V
T11523 22317-22318 HYPH denotes -
T11520 22318-22321 NN denotes GFP
T11525 22322-22324 VBZ denotes is
T11524 22325-22334 VBN denotes expressed
T11526 22335-22348 RB denotes approximately
T11527 22349-22356 RB denotes equally
T11528 22357-22359 IN denotes in
T11529 22360-22364 DT denotes both
T11531 22365-22369 NN denotes cell
T11530 22370-22375 NNS denotes lines
T11532 22376-22377 -LRB- denotes (
T11534 22377-22383 NN denotes Figure
T11535 22384-22386 CD denotes 5D
T11536 22386-22388 , denotes ,
T11537 22388-22393 JJ denotes total
T11533 22394-22399 NNS denotes cells
T11538 22399-22400 -RRB- denotes )
T11539 22400-22402 , denotes ,
T11540 22402-22405 CC denotes but
T11541 22406-22408 VBZ denotes is
T11542 22409-22421 VBN denotes biotinylated
T11543 22422-22426 RB denotes only
T11545 22427-22431 WRB denotes when
T11546 22432-22436 JJ denotes wild
T11548 22436-22437 HYPH denotes -
T11547 22437-22441 NN denotes type
T11549 22442-22446 NN denotes AQP2
T11550 22447-22449 VBZ denotes is
T11551 22450-22454 RB denotes also
T11544 22455-22464 VBN denotes expressed
T11552 22465-22466 -LRB- denotes (
T11553 22466-22472 NN denotes Figure
T11554 22473-22475 CD denotes 5D
T11555 22475-22477 , denotes ,
T11556 22477-22484 NN denotes surface
T11557 22485-22497 VBN denotes biotinylated
T11558 22497-22498 -RRB- denotes )
T11559 22498-22499 . denotes .
T11560 22499-22684 sentence denotes Since only cell surface proteins are accessible to biotin, these results indicate that AQP2-F204V is transported to the cell surface when wild-type AQP2 is present, but not on its own.
T11561 22500-22505 IN denotes Since
T11563 22506-22510 RB denotes only
T11565 22511-22515 NN denotes cell
T11566 22516-22523 NN denotes surface
T11564 22524-22532 NN denotes proteins
T11562 22533-22536 VBP denotes are
T11568 22537-22547 JJ denotes accessible
T11569 22548-22550 IN denotes to
T11570 22551-22557 NN denotes biotin
T11571 22557-22559 , denotes ,
T11572 22559-22564 DT denotes these
T11573 22565-22572 NNS denotes results
T11567 22573-22581 VBP denotes indicate
T11574 22582-22586 IN denotes that
T11576 22587-22591 NN denotes AQP2
T11578 22591-22592 HYPH denotes -
T11577 22592-22597 NN denotes F204V
T11579 22598-22600 VBZ denotes is
T11575 22601-22612 VBN denotes transported
T11580 22613-22615 IN denotes to
T11581 22616-22619 DT denotes the
T11583 22620-22624 NN denotes cell
T11582 22625-22632 NN denotes surface
T11584 22633-22637 WRB denotes when
T11586 22638-22642 JJ denotes wild
T11588 22642-22643 HYPH denotes -
T11587 22643-22647 NN denotes type
T11589 22648-22652 NN denotes AQP2
T11585 22653-22655 VBZ denotes is
T11590 22656-22663 JJ denotes present
T11591 22663-22665 , denotes ,
T11592 22665-22668 CC denotes but
T11593 22669-22672 RB denotes not
T11594 22673-22675 IN denotes on
T11595 22676-22679 PRP$ denotes its
T11596 22680-22683 JJ denotes own
T11597 22683-22684 . denotes .
T14172 22697-22705 NN denotes Aqp2F204
T14174 22705-22706 HYPH denotes /
T14173 22706-22711 NN denotes F204V
T14175 22712-22716 NNS denotes mice
T14176 22717-22720 VBP denotes are
T14177 22721-22727 JJ denotes viable
T14178 22728-22731 CC denotes and
T14179 22732-22736 VBP denotes grow
T14180 22737-22740 CC denotes and
T14181 22741-22750 VBP denotes reproduce
T14182 22751-22759 RB denotes normally
T14183 22759-22760 . denotes .
T14184 22760-22959 sentence denotes They are, however, severely defective in their ability to concentrate urine, leading to increased urine output and water intake, thus making them the first mouse model of NDI to survive to maturity.
T14185 22761-22765 PRP denotes They
T14186 22766-22769 VBP denotes are
T14187 22769-22771 , denotes ,
T14188 22771-22778 RB denotes however
T14189 22778-22780 , denotes ,
T14190 22780-22788 RB denotes severely
T14191 22789-22798 JJ denotes defective
T14192 22799-22801 IN denotes in
T14193 22802-22807 PRP$ denotes their
T14194 22808-22815 NN denotes ability
T14195 22816-22818 TO denotes to
T14196 22819-22830 VB denotes concentrate
T14197 22831-22836 NN denotes urine
T14198 22836-22838 , denotes ,
T14199 22838-22845 VBG denotes leading
T14200 22846-22848 IN denotes to
T14201 22849-22858 VBN denotes increased
T14203 22859-22864 NN denotes urine
T14202 22865-22871 NN denotes output
T14204 22872-22875 CC denotes and
T14205 22876-22881 NN denotes water
T14206 22882-22888 NN denotes intake
T14207 22888-22890 , denotes ,
T14208 22890-22894 RB denotes thus
T14209 22895-22901 VBG denotes making
T14210 22902-22906 PRP denotes them
T14212 22907-22910 DT denotes the
T14213 22911-22916 JJ denotes first
T14214 22917-22922 NN denotes mouse
T14211 22923-22928 NN denotes model
T14215 22929-22931 IN denotes of
T14216 22932-22935 NN denotes NDI
T14217 22936-22938 TO denotes to
T14218 22939-22946 VB denotes survive
T14219 22947-22949 IN denotes to
T14220 22950-22958 NN denotes maturity
T14221 22958-22959 . denotes .
T14222 22959-23015 sentence denotes In humans, NDI is caused by mutations in Avpr2 or Aqp2.
T14223 22960-22962 IN denotes In
T14225 22963-22969 NNS denotes humans
T14226 22969-22971 , denotes ,
T14227 22971-22974 NN denotes NDI
T14228 22975-22977 VBZ denotes is
T14224 22978-22984 VBN denotes caused
T14229 22985-22987 IN denotes by
T14230 22988-22997 NNS denotes mutations
T14231 22998-23000 IN denotes in
T14232 23001-23006 NN denotes Avpr2
T14233 23007-23009 CC denotes or
T14234 23010-23014 NN denotes Aqp2
T14235 23014-23015 . denotes .
T14236 23015-23210 sentence denotes Knockout of the X-linked Avpr2 gene in mice [15] gave an NDI-like phenotype in male, hemizygous neonates, but the phenotype could not be assessed in adults as the mice died within 1 wk of birth.
T14237 23016-23024 NN denotes Knockout
T14239 23025-23027 IN denotes of
T14240 23028-23031 DT denotes the
T14242 23032-23033 NN denotes X
T14244 23033-23034 HYPH denotes -
T14243 23034-23040 VBN denotes linked
T14245 23041-23046 NN denotes Avpr2
T14241 23047-23051 NN denotes gene
T14246 23052-23054 IN denotes in
T14247 23055-23059 NNS denotes mice
T14248 23060-23061 -LRB- denotes [
T14249 23061-23063 CD denotes 15
T14250 23063-23064 -RRB- denotes ]
T14238 23065-23069 VBD denotes gave
T14251 23070-23072 DT denotes an
T14253 23073-23076 NN denotes NDI
T14255 23076-23077 HYPH denotes -
T14254 23077-23081 JJ denotes like
T14252 23082-23091 NN denotes phenotype
T14256 23092-23094 IN denotes in
T14257 23095-23099 JJ denotes male
T14259 23099-23101 , denotes ,
T14260 23101-23111 JJ denotes hemizygous
T14258 23112-23120 NNS denotes neonates
T14261 23120-23122 , denotes ,
T14262 23122-23125 CC denotes but
T14263 23126-23129 DT denotes the
T14264 23130-23139 NN denotes phenotype
T14266 23140-23145 MD denotes could
T14267 23146-23149 RB denotes not
T14268 23150-23152 VB denotes be
T14265 23153-23161 VBN denotes assessed
T14269 23162-23164 IN denotes in
T14270 23165-23171 NNS denotes adults
T14271 23172-23174 IN denotes as
T14273 23175-23178 DT denotes the
T14274 23179-23183 NNS denotes mice
T14272 23184-23188 VBD denotes died
T14275 23189-23195 IN denotes within
T14276 23196-23197 CD denotes 1
T14277 23198-23200 NN denotes wk
T14278 23201-23203 IN denotes of
T14279 23204-23209 NN denotes birth
T14280 23209-23210 . denotes .
T14281 23210-23345 sentence denotes The adult heterozygous females showed a mild tendency toward increased urinary output and water intake and decreased urine osmolality.
T14282 23211-23214 DT denotes The
T14284 23215-23220 JJ denotes adult
T14285 23221-23233 JJ denotes heterozygous
T14283 23234-23241 NNS denotes females
T14286 23242-23248 VBD denotes showed
T14287 23249-23250 DT denotes a
T14289 23251-23255 JJ denotes mild
T14288 23256-23264 NN denotes tendency
T14290 23265-23271 IN denotes toward
T14291 23272-23281 VBN denotes increased
T14293 23282-23289 JJ denotes urinary
T14292 23290-23296 NN denotes output
T14294 23297-23300 CC denotes and
T14295 23301-23306 NN denotes water
T14296 23307-23313 NN denotes intake
T14297 23314-23317 CC denotes and
T14298 23318-23327 VBN denotes decreased
T14300 23328-23333 NN denotes urine
T14299 23334-23344 NN denotes osmolality
T14301 23344-23345 . denotes .
T14302 23345-23400 sentence denotes Knockout of the mouse Aqp2 gene has not been reported.
T14303 23346-23354 NN denotes Knockout
T14305 23355-23357 IN denotes of
T14306 23358-23361 DT denotes the
T14308 23362-23367 NN denotes mouse
T14309 23368-23372 NN denotes Aqp2
T14307 23373-23377 NN denotes gene
T14310 23378-23381 VBZ denotes has
T14311 23382-23385 RB denotes not
T14312 23386-23390 VBN denotes been
T14304 23391-23399 VBN denotes reported
T14313 23399-23400 . denotes .
T14314 23400-23485 sentence denotes A knock-in of a human disease-causing mutation (T126M), however, has been made [18].
T14315 23401-23402 DT denotes A
T14317 23403-23408 NN denotes knock
T14318 23408-23409 HYPH denotes -
T14316 23409-23411 NN denotes in
T14320 23412-23414 IN denotes of
T14321 23415-23416 DT denotes a
T14323 23417-23422 JJ denotes human
T14324 23423-23430 NN denotes disease
T14326 23430-23431 HYPH denotes -
T14325 23431-23438 VBG denotes causing
T14322 23439-23447 NN denotes mutation
T14327 23448-23449 -LRB- denotes (
T14328 23449-23454 NN denotes T126M
T14329 23454-23455 -RRB- denotes )
T14330 23455-23457 , denotes ,
T14331 23457-23464 RB denotes however
T14332 23464-23466 , denotes ,
T14333 23466-23469 VBZ denotes has
T14334 23470-23474 VBN denotes been
T14319 23475-23479 VBN denotes made
T14335 23480-23481 -LRB- denotes [
T14336 23481-23483 CD denotes 18
T14337 23483-23484 -RRB- denotes ]
T14338 23484-23485 . denotes .
T14339 23485-23594 sentence denotes These mice have a severe urine-concentrating defect resulting in dehydration and death within 1 wk of birth.
T14340 23486-23491 DT denotes These
T14341 23492-23496 NNS denotes mice
T14342 23497-23501 VBP denotes have
T14343 23502-23503 DT denotes a
T14345 23504-23510 JJ denotes severe
T14346 23511-23516 NN denotes urine
T14348 23516-23517 HYPH denotes -
T14347 23517-23530 VBG denotes concentrating
T14344 23531-23537 NN denotes defect
T14349 23538-23547 VBG denotes resulting
T14350 23548-23550 IN denotes in
T14351 23551-23562 NN denotes dehydration
T14352 23563-23566 CC denotes and
T14353 23567-23572 NN denotes death
T14354 23573-23579 IN denotes within
T14355 23580-23581 CD denotes 1
T14356 23582-23584 NN denotes wk
T14357 23585-23587 IN denotes of
T14358 23588-23593 NN denotes birth
T14359 23593-23594 . denotes .
T14360 23594-23670 sentence denotes Curiously, AQP2-T126M does localize properly in at least a subset of cells.
T14361 23595-23604 RB denotes Curiously
T14363 23604-23606 , denotes ,
T14364 23606-23610 NN denotes AQP2
T14366 23610-23611 HYPH denotes -
T14365 23611-23616 NN denotes T126M
T14367 23617-23621 VBZ denotes does
T14362 23622-23630 VB denotes localize
T14368 23631-23639 RB denotes properly
T14369 23640-23642 IN denotes in
T14370 23643-23645 RB denotes at
T14371 23646-23651 RBS denotes least
T14373 23652-23653 DT denotes a
T14372 23654-23660 NN denotes subset
T14374 23661-23663 IN denotes of
T14375 23664-23669 NNS denotes cells
T14376 23669-23670 . denotes .
T14377 23670-23795 sentence denotes The grossly abnormal collecting duct morphology makes it impossible to pinpoint the molecular defect in these knock-in mice.
T14378 23671-23674 DT denotes The
T14380 23675-23682 RB denotes grossly
T14381 23683-23691 JJ denotes abnormal
T14382 23692-23702 VBG denotes collecting
T14383 23703-23707 NN denotes duct
T14379 23708-23718 NN denotes morphology
T14384 23719-23724 VBZ denotes makes
T14385 23725-23727 PRP denotes it
T14386 23728-23738 JJ denotes impossible
T14387 23739-23741 TO denotes to
T14388 23742-23750 VB denotes pinpoint
T14389 23751-23754 DT denotes the
T14391 23755-23764 JJ denotes molecular
T14390 23765-23771 NN denotes defect
T14392 23772-23774 IN denotes in
T14393 23775-23780 DT denotes these
T14395 23781-23786 VB denotes knock
T14396 23786-23787 HYPH denotes -
T14397 23787-23789 RP denotes in
T14394 23790-23794 NNS denotes mice
T14398 23794-23795 . denotes .
T14399 23795-23865 sentence denotes The T126M knock-in clearly shows that Aqp2 is an essential gene [18].
T14400 23796-23799 DT denotes The
T14402 23800-23805 NN denotes T126M
T14403 23806-23811 NN denotes knock
T14404 23811-23812 HYPH denotes -
T14401 23812-23814 NN denotes in
T14406 23815-23822 RB denotes clearly
T14405 23823-23828 VBZ denotes shows
T14407 23829-23833 IN denotes that
T14409 23834-23838 NN denotes Aqp2
T14408 23839-23841 VBZ denotes is
T14410 23842-23844 DT denotes an
T14412 23845-23854 JJ denotes essential
T14411 23855-23859 NN denotes gene
T14413 23860-23861 -LRB- denotes [
T14414 23861-23863 CD denotes 18
T14415 23863-23864 -RRB- denotes ]
T14416 23864-23865 . denotes .
T14417 23865-24043 sentence denotes The fact that our mice survive shows either that AQP2-F204V possesses some residual water transporting ability or that there are AVP-independent pathways for water reabsorption.
T14418 23866-23869 DT denotes The
T14419 23870-23874 NN denotes fact
T14421 23875-23879 IN denotes that
T14423 23880-23883 PRP$ denotes our
T14424 23884-23888 NNS denotes mice
T14422 23889-23896 VBP denotes survive
T14420 23897-23902 VBZ denotes shows
T14425 23903-23909 CC denotes either
T14427 23910-23914 IN denotes that
T14428 23915-23919 NN denotes AQP2
T14430 23919-23920 HYPH denotes -
T14429 23920-23925 NN denotes F204V
T14426 23926-23935 VBZ denotes possesses
T14431 23936-23940 DT denotes some
T14433 23941-23949 JJ denotes residual
T14434 23950-23955 NN denotes water
T14435 23956-23968 VBG denotes transporting
T14432 23969-23976 NN denotes ability
T14436 23977-23979 CC denotes or
T14437 23980-23984 IN denotes that
T14439 23985-23990 EX denotes there
T14438 23991-23994 VBP denotes are
T14440 23995-23998 NN denotes AVP
T14442 23998-23999 HYPH denotes -
T14441 23999-24010 JJ denotes independent
T14443 24011-24019 NNS denotes pathways
T14444 24020-24023 IN denotes for
T14445 24024-24029 NN denotes water
T14446 24030-24042 NN denotes reabsorption
T14447 24042-24043 . denotes .
T14448 24043-24201 sentence denotes Residual activity of AQP2-F204V is likely, as mutant animals show some small response to dDAVP, although dDAVP-stimulated urine osmolality remains quite low.
T14449 24044-24052 JJ denotes Residual
T14450 24053-24061 NN denotes activity
T14452 24062-24064 IN denotes of
T14453 24065-24069 NN denotes AQP2
T14455 24069-24070 HYPH denotes -
T14454 24070-24075 NN denotes F204V
T14451 24076-24078 VBZ denotes is
T14456 24079-24085 JJ denotes likely
T14457 24085-24087 , denotes ,
T14458 24087-24089 IN denotes as
T14460 24090-24096 NN denotes mutant
T14461 24097-24104 NNS denotes animals
T14459 24105-24109 VBP denotes show
T14462 24110-24114 DT denotes some
T14464 24115-24120 JJ denotes small
T14463 24121-24129 NN denotes response
T14465 24130-24132 IN denotes to
T14466 24133-24138 NN denotes dDAVP
T14467 24138-24140 , denotes ,
T14468 24140-24148 IN denotes although
T14470 24149-24154 NN denotes dDAVP
T14472 24154-24155 HYPH denotes -
T14471 24155-24165 VBN denotes stimulated
T14474 24166-24171 NN denotes urine
T14473 24172-24182 NN denotes osmolality
T14469 24183-24190 VBZ denotes remains
T14475 24191-24196 RB denotes quite
T14476 24197-24200 JJ denotes low
T14477 24200-24201 . denotes .
T14478 24201-24365 sentence denotes Immunostaining of kidney shows that AQP2-F204V does not efficiently transport water, because it fails to localize to the apical cell surface after dDAVP treatment.
T14479 24202-24216 NN denotes Immunostaining
T14481 24217-24219 IN denotes of
T14482 24220-24226 NN denotes kidney
T14480 24227-24232 VBZ denotes shows
T14483 24233-24237 IN denotes that
T14485 24238-24242 NN denotes AQP2
T14487 24242-24243 HYPH denotes -
T14486 24243-24248 NN denotes F204V
T14488 24249-24253 VBZ denotes does
T14489 24254-24257 RB denotes not
T14490 24258-24269 RB denotes efficiently
T14484 24270-24279 VB denotes transport
T14491 24280-24285 NN denotes water
T14492 24285-24287 , denotes ,
T14493 24287-24294 IN denotes because
T14495 24295-24297 PRP denotes it
T14494 24298-24303 VBZ denotes fails
T14496 24304-24306 TO denotes to
T14497 24307-24315 VB denotes localize
T14498 24316-24318 IN denotes to
T14499 24319-24322 DT denotes the
T14501 24323-24329 JJ denotes apical
T14502 24330-24334 NN denotes cell
T14500 24335-24342 NN denotes surface
T14503 24343-24348 IN denotes after
T14504 24349-24354 NN denotes dDAVP
T14505 24355-24364 NN denotes treatment
T14506 24364-24365 . denotes .
T14507 24365-24500 sentence denotes Some residual activity of AQP2 would imply that some small, undetectable portion of the mutant protein is getting to the cell surface.
T14508 24366-24370 DT denotes Some
T14510 24371-24379 JJ denotes residual
T14509 24380-24388 NN denotes activity
T14512 24389-24391 IN denotes of
T14513 24392-24396 NN denotes AQP2
T14514 24397-24402 MD denotes would
T14511 24403-24408 VB denotes imply
T14515 24409-24413 IN denotes that
T14517 24414-24418 DT denotes some
T14519 24419-24424 JJ denotes small
T14520 24424-24426 , denotes ,
T14521 24426-24438 JJ denotes undetectable
T14518 24439-24446 NN denotes portion
T14522 24447-24449 IN denotes of
T14523 24450-24453 DT denotes the
T14525 24454-24460 NN denotes mutant
T14524 24461-24468 NN denotes protein
T14526 24469-24471 VBZ denotes is
T14516 24472-24479 VBG denotes getting
T14527 24480-24482 IN denotes to
T14528 24483-24486 DT denotes the
T14530 24487-24491 NN denotes cell
T14529 24492-24499 NN denotes surface
T14531 24499-24500 . denotes .
T14532 24500-24663 sentence denotes The surface biotinylation experiment (Figure 5D) suggests that no mutant protein gets to the surface, but this does not necessarily reflect the situation in vivo.
T14533 24501-24504 DT denotes The
T14535 24505-24512 NN denotes surface
T14536 24513-24526 NN denotes biotinylation
T14534 24527-24537 NN denotes experiment
T14538 24538-24539 -LRB- denotes (
T14539 24539-24545 NN denotes Figure
T14540 24546-24548 CD denotes 5D
T14541 24548-24549 -RRB- denotes )
T14537 24550-24558 VBZ denotes suggests
T14542 24559-24563 IN denotes that
T14544 24564-24566 DT denotes no
T14546 24567-24573 NN denotes mutant
T14545 24574-24581 NN denotes protein
T14543 24582-24586 VBZ denotes gets
T14547 24587-24589 IN denotes to
T14548 24590-24593 DT denotes the
T14549 24594-24601 NN denotes surface
T14550 24601-24603 , denotes ,
T14551 24603-24606 CC denotes but
T14552 24607-24611 DT denotes this
T14554 24612-24616 VBZ denotes does
T14555 24617-24620 RB denotes not
T14556 24621-24632 RB denotes necessarily
T14553 24633-24640 VB denotes reflect
T14557 24641-24644 DT denotes the
T14558 24645-24654 NN denotes situation
T14559 24655-24657 FW denotes in
T14560 24658-24662 FW denotes vivo
T14561 24662-24663 . denotes .
T14562 24663-24855 sentence denotes While this small fraction of protein may not be detectable by immunofluorescence, Western blotting shows that some mutant protein does progress beyond the ER (35–45 kDa species in Figure 3A).
T14563 24664-24669 IN denotes While
T14565 24670-24674 DT denotes this
T14567 24675-24680 JJ denotes small
T14566 24681-24689 NN denotes fraction
T14568 24690-24692 IN denotes of
T14569 24693-24700 NN denotes protein
T14570 24701-24704 MD denotes may
T14571 24705-24708 RB denotes not
T14564 24709-24711 VB denotes be
T14573 24712-24722 JJ denotes detectable
T14574 24723-24725 IN denotes by
T14575 24726-24744 NN denotes immunofluorescence
T14576 24744-24746 , denotes ,
T14577 24746-24753 NNP denotes Western
T14578 24754-24762 NN denotes blotting
T14572 24763-24768 VBZ denotes shows
T14579 24769-24773 IN denotes that
T14581 24774-24778 DT denotes some
T14583 24779-24785 NN denotes mutant
T14582 24786-24793 NN denotes protein
T14584 24794-24798 VBZ denotes does
T14580 24799-24807 VB denotes progress
T14585 24808-24814 IN denotes beyond
T14586 24815-24818 DT denotes the
T14587 24819-24821 NN denotes ER
T14588 24822-24823 -LRB- denotes (
T14590 24823-24825 CD denotes 35
T14592 24825-24826 SYM denotes –
T14591 24826-24828 CD denotes 45
T14593 24829-24832 NN denotes kDa
T14589 24833-24840 NNS denotes species
T14594 24841-24843 IN denotes in
T14595 24844-24850 NN denotes Figure
T14596 24851-24853 CD denotes 3A
T14597 24853-24854 -RRB- denotes )
T14598 24854-24855 . denotes .
T14599 24855-25044 sentence denotes Compared to wild-type, mutant protein is enriched in the high-mannose, core-glycosylated form (31 kDa) and deficient in nonglycosylated (29 kDa) and complex glycosylated (35–45 kDa) forms.
T14600 24856-24864 VBN denotes Compared
T14602 24865-24867 IN denotes to
T14603 24868-24872 JJ denotes wild
T14605 24872-24873 HYPH denotes -
T14604 24873-24877 NN denotes type
T14606 24877-24879 , denotes ,
T14607 24879-24885 NN denotes mutant
T14608 24886-24893 NN denotes protein
T14601 24894-24896 VBZ denotes is
T14609 24897-24905 VBN denotes enriched
T14610 24906-24908 IN denotes in
T14611 24909-24912 DT denotes the
T14613 24913-24917 JJ denotes high
T14615 24917-24918 HYPH denotes -
T14614 24918-24925 NN denotes mannose
T14616 24925-24927 , denotes ,
T14617 24927-24931 NN denotes core
T14619 24931-24932 HYPH denotes -
T14618 24932-24944 VBN denotes glycosylated
T14612 24945-24949 NN denotes form
T14620 24950-24951 -LRB- denotes (
T14622 24951-24953 CD denotes 31
T14621 24954-24957 NN denotes kDa
T14623 24957-24958 -RRB- denotes )
T14624 24959-24962 CC denotes and
T14625 24963-24972 JJ denotes deficient
T14626 24973-24975 IN denotes in
T14627 24976-24991 JJ denotes nonglycosylated
T14629 24992-24993 -LRB- denotes (
T14631 24993-24995 CD denotes 29
T14630 24996-24999 NN denotes kDa
T14632 24999-25000 -RRB- denotes )
T14633 25001-25004 CC denotes and
T14634 25005-25012 JJ denotes complex
T14635 25013-25025 JJ denotes glycosylated
T14636 25026-25027 -LRB- denotes (
T14638 25027-25029 CD denotes 35
T14640 25029-25030 SYM denotes –
T14639 25030-25032 CD denotes 45
T14637 25033-25036 NN denotes kDa
T14641 25036-25037 -RRB- denotes )
T14628 25038-25043 NNS denotes forms
T14642 25043-25044 . denotes .
T14643 25044-25223 sentence denotes The presence of a reduced but detectable amount of protein in the 35–45 kDa range indicates that mutant protein is transported out of the ER, but with greatly reduced efficiency.
T14644 25045-25048 DT denotes The
T14645 25049-25057 NN denotes presence
T14647 25058-25060 IN denotes of
T14648 25061-25062 DT denotes a
T14650 25063-25070 VBN denotes reduced
T14651 25071-25074 CC denotes but
T14652 25075-25085 JJ denotes detectable
T14649 25086-25092 NN denotes amount
T14653 25093-25095 IN denotes of
T14654 25096-25103 NN denotes protein
T14655 25104-25106 IN denotes in
T14656 25107-25110 DT denotes the
T14658 25111-25113 CD denotes 35
T14660 25113-25114 SYM denotes –
T14659 25114-25116 CD denotes 45
T14661 25117-25120 NN denotes kDa
T14657 25121-25126 NN denotes range
T14646 25127-25136 VBZ denotes indicates
T14662 25137-25141 IN denotes that
T14664 25142-25148 NN denotes mutant
T14665 25149-25156 NN denotes protein
T14666 25157-25159 VBZ denotes is
T14663 25160-25171 VBN denotes transported
T14667 25172-25175 IN denotes out
T14668 25176-25178 IN denotes of
T14669 25179-25182 DT denotes the
T14670 25183-25185 NN denotes ER
T14671 25185-25187 , denotes ,
T14672 25187-25190 CC denotes but
T14673 25191-25195 IN denotes with
T14674 25196-25203 RB denotes greatly
T14675 25204-25211 VBN denotes reduced
T14676 25212-25222 NN denotes efficiency
T14677 25222-25223 . denotes .
T14678 25223-25408 sentence denotes Colocalization of AQP2-F204V with the ER protein calnexin in transfected MDCK cells shows that, while most of the mutant protein is trapped in the ER, some does progress beyond the ER.
T14679 25224-25238 NN denotes Colocalization
T14681 25239-25241 IN denotes of
T14682 25242-25246 NN denotes AQP2
T14684 25246-25247 HYPH denotes -
T14683 25247-25252 NN denotes F204V
T14685 25253-25257 IN denotes with
T14686 25258-25261 DT denotes the
T14688 25262-25264 NN denotes ER
T14687 25265-25272 NN denotes protein
T14689 25273-25281 NN denotes calnexin
T14690 25282-25284 IN denotes in
T14691 25285-25296 VBN denotes transfected
T14693 25297-25301 NN denotes MDCK
T14692 25302-25307 NNS denotes cells
T14680 25308-25313 VBZ denotes shows
T14694 25314-25318 IN denotes that
T14696 25318-25320 , denotes ,
T14697 25320-25325 IN denotes while
T14699 25326-25330 JJS denotes most
T14700 25331-25333 IN denotes of
T14701 25334-25337 DT denotes the
T14703 25338-25344 NN denotes mutant
T14702 25345-25352 NN denotes protein
T14704 25353-25355 VBZ denotes is
T14698 25356-25363 VBN denotes trapped
T14705 25364-25366 IN denotes in
T14706 25367-25370 DT denotes the
T14707 25371-25373 NN denotes ER
T14708 25373-25375 , denotes ,
T14709 25375-25379 DT denotes some
T14710 25380-25384 VBZ denotes does
T14695 25385-25393 VB denotes progress
T14711 25394-25400 IN denotes beyond
T14712 25401-25404 DT denotes the
T14713 25405-25407 NN denotes ER
T14714 25407-25408 . denotes .
T14715 25408-25726 sentence denotes Diminished response to dDAVP, diminished abundance of mature glycosylated protein in mutant animals, and the transport of a fraction of mutant protein beyond the ER in MDCK cells are all consistent with the notion that AQP2-F204V misfolding is limited and that it may retain some residual water transporting activity.
T14716 25409-25419 VBN denotes Diminished
T14717 25420-25428 NN denotes response
T14719 25429-25431 IN denotes to
T14720 25432-25437 NN denotes dDAVP
T14721 25437-25439 , denotes ,
T14722 25439-25449 VBN denotes diminished
T14723 25450-25459 NN denotes abundance
T14724 25460-25462 IN denotes of
T14725 25463-25469 JJ denotes mature
T14727 25470-25482 VBN denotes glycosylated
T14726 25483-25490 NN denotes protein
T14728 25491-25493 IN denotes in
T14729 25494-25500 NN denotes mutant
T14730 25501-25508 NNS denotes animals
T14731 25508-25510 , denotes ,
T14732 25510-25513 CC denotes and
T14733 25514-25517 DT denotes the
T14734 25518-25527 NN denotes transport
T14735 25528-25530 IN denotes of
T14736 25531-25532 DT denotes a
T14737 25533-25541 NN denotes fraction
T14738 25542-25544 IN denotes of
T14739 25545-25551 NN denotes mutant
T14740 25552-25559 NN denotes protein
T14741 25560-25566 IN denotes beyond
T14742 25567-25570 DT denotes the
T14743 25571-25573 NN denotes ER
T14744 25574-25576 IN denotes in
T14745 25577-25581 NN denotes MDCK
T14746 25582-25587 NNS denotes cells
T14718 25588-25591 VBP denotes are
T14747 25592-25595 RB denotes all
T14748 25596-25606 JJ denotes consistent
T14749 25607-25611 IN denotes with
T14750 25612-25615 DT denotes the
T14751 25616-25622 NN denotes notion
T14752 25623-25627 IN denotes that
T14754 25628-25632 NN denotes AQP2
T14756 25632-25633 HYPH denotes -
T14755 25633-25638 NN denotes F204V
T14757 25639-25649 NN denotes misfolding
T14753 25650-25652 VBZ denotes is
T14758 25653-25660 JJ denotes limited
T14759 25661-25664 CC denotes and
T14760 25665-25669 IN denotes that
T14762 25670-25672 PRP denotes it
T14763 25673-25676 MD denotes may
T14761 25677-25683 VB denotes retain
T14764 25684-25688 DT denotes some
T14766 25689-25697 JJ denotes residual
T14767 25698-25703 NN denotes water
T14768 25704-25716 VBG denotes transporting
T14765 25717-25725 NN denotes activity
T14769 25725-25726 . denotes .
T14770 25726-25821 sentence denotes Evidently this residual activity is sufficient for the viability and growth of mutant animals.
T14771 25727-25736 RB denotes Evidently
T14773 25737-25741 DT denotes this
T14775 25742-25750 JJ denotes residual
T14774 25751-25759 NN denotes activity
T14772 25760-25762 VBZ denotes is
T14776 25763-25773 JJ denotes sufficient
T14777 25774-25777 IN denotes for
T14778 25778-25781 DT denotes the
T14779 25782-25791 NN denotes viability
T14780 25792-25795 CC denotes and
T14781 25796-25802 NN denotes growth
T14782 25803-25805 IN denotes of
T14783 25806-25812 NN denotes mutant
T14784 25813-25820 NNS denotes animals
T14785 25820-25821 . denotes .
T14786 25821-25943 sentence denotes Reduced efficiency in exiting the ER may explain why AQP2-F204V is enriched in the 31 kDa high-mannose glycosylated form.
T14787 25822-25829 VBN denotes Reduced
T14788 25830-25840 NN denotes efficiency
T14790 25841-25843 IN denotes in
T14791 25844-25851 VBG denotes exiting
T14792 25852-25855 DT denotes the
T14793 25856-25858 NN denotes ER
T14794 25859-25862 MD denotes may
T14789 25863-25870 VB denotes explain
T14795 25871-25874 WRB denotes why
T14797 25875-25879 NN denotes AQP2
T14799 25879-25880 HYPH denotes -
T14798 25880-25885 NN denotes F204V
T14800 25886-25888 VBZ denotes is
T14796 25889-25897 VBN denotes enriched
T14801 25898-25900 IN denotes in
T14802 25901-25904 DT denotes the
T14804 25905-25907 CD denotes 31
T14805 25908-25911 NN denotes kDa
T14806 25912-25916 JJ denotes high
T14808 25916-25917 HYPH denotes -
T14807 25917-25924 NN denotes mannose
T14809 25925-25937 VBN denotes glycosylated
T14803 25938-25942 NN denotes form
T14810 25942-25943 . denotes .
T14811 25943-26066 sentence denotes The high-mannose core oligosaccharide is added in the ER and is later modified and elaborated in the Golgi apparatus [26].
T14812 25944-25947 DT denotes The
T14814 25948-25952 JJ denotes high
T14816 25952-25953 HYPH denotes -
T14815 25953-25960 NN denotes mannose
T14817 25961-25965 NN denotes core
T14813 25966-25981 NN denotes oligosaccharide
T14819 25982-25984 VBZ denotes is
T14818 25985-25990 VBN denotes added
T14820 25991-25993 IN denotes in
T14821 25994-25997 DT denotes the
T14822 25998-26000 NN denotes ER
T14823 26001-26004 CC denotes and
T14824 26005-26007 VBZ denotes is
T14826 26008-26013 RB denotes later
T14825 26014-26022 VBN denotes modified
T14827 26023-26026 CC denotes and
T14828 26027-26037 VBN denotes elaborated
T14829 26038-26040 IN denotes in
T14830 26041-26044 DT denotes the
T14832 26045-26050 NN denotes Golgi
T14831 26051-26060 NN denotes apparatus
T14833 26061-26062 -LRB- denotes [
T14834 26062-26064 CD denotes 26
T14835 26064-26065 -RRB- denotes ]
T14836 26065-26066 . denotes .
T14837 26066-26227 sentence denotes The increase in the high-mannose glycosylated form of AQP2-F204V may simply reflect its prolonged presence in the ER and exposure to oligosaccharyl transferase.
T14838 26067-26070 DT denotes The
T14839 26071-26079 NN denotes increase
T14841 26080-26082 IN denotes in
T14842 26083-26086 DT denotes the
T14844 26087-26091 JJ denotes high
T14846 26091-26092 HYPH denotes -
T14845 26092-26099 NN denotes mannose
T14847 26100-26112 VBN denotes glycosylated
T14843 26113-26117 NN denotes form
T14848 26118-26120 IN denotes of
T14849 26121-26125 NN denotes AQP2
T14851 26125-26126 HYPH denotes -
T14850 26126-26131 NN denotes F204V
T14852 26132-26135 MD denotes may
T14853 26136-26142 RB denotes simply
T14840 26143-26150 VB denotes reflect
T14854 26151-26154 PRP$ denotes its
T14856 26155-26164 JJ denotes prolonged
T14855 26165-26173 NN denotes presence
T14857 26174-26176 IN denotes in
T14858 26177-26180 DT denotes the
T14859 26181-26183 NN denotes ER
T14860 26184-26187 CC denotes and
T14861 26188-26196 NN denotes exposure
T14862 26197-26199 IN denotes to
T14863 26200-26214 NN denotes oligosaccharyl
T14864 26215-26226 NN denotes transferase
T14865 26226-26227 . denotes .
T14866 26227-26397 sentence denotes While improper localization of AQP2 explains the phenotype of homozygous mutant mice, the complete lack of a phenotype in heterozygous mice is more difficult to explain.
T14867 26228-26233 IN denotes While
T14869 26234-26242 JJ denotes improper
T14870 26243-26255 NN denotes localization
T14871 26256-26258 IN denotes of
T14872 26259-26263 NN denotes AQP2
T14868 26264-26272 VBZ denotes explains
T14874 26273-26276 DT denotes the
T14875 26277-26286 NN denotes phenotype
T14876 26287-26289 IN denotes of
T14877 26290-26300 JJ denotes homozygous
T14879 26301-26307 NN denotes mutant
T14878 26308-26312 NNS denotes mice
T14880 26312-26314 , denotes ,
T14881 26314-26317 DT denotes the
T14883 26318-26326 JJ denotes complete
T14882 26327-26331 NN denotes lack
T14884 26332-26334 IN denotes of
T14885 26335-26336 DT denotes a
T14886 26337-26346 NN denotes phenotype
T14887 26347-26349 IN denotes in
T14888 26350-26362 JJ denotes heterozygous
T14889 26363-26367 NNS denotes mice
T14873 26368-26370 VBZ denotes is
T14890 26371-26375 RBR denotes more
T14891 26376-26385 JJ denotes difficult
T14892 26386-26388 TO denotes to
T14893 26389-26396 VB denotes explain
T14894 26396-26397 . denotes .
T14895 26397-26554 sentence denotes Physiologically, heterozygous mice have no symptoms (see Figure 1C), and they are indistinguishable from wild type on immunostaining of kidneys (Figure 5A).
T14896 26398-26413 RB denotes Physiologically
T14898 26413-26415 , denotes ,
T14899 26415-26427 JJ denotes heterozygous
T14900 26428-26432 NNS denotes mice
T14897 26433-26437 VBP denotes have
T14901 26438-26440 DT denotes no
T14902 26441-26449 NNS denotes symptoms
T14903 26450-26451 -LRB- denotes (
T14904 26451-26454 VB denotes see
T14905 26455-26461 NN denotes Figure
T14906 26462-26464 CD denotes 1C
T14907 26464-26465 -RRB- denotes )
T14908 26465-26467 , denotes ,
T14909 26467-26470 CC denotes and
T14910 26471-26475 PRP denotes they
T14911 26476-26479 VBP denotes are
T14912 26480-26497 JJ denotes indistinguishable
T14913 26498-26502 IN denotes from
T14914 26503-26507 JJ denotes wild
T14915 26508-26512 NN denotes type
T14916 26513-26515 IN denotes on
T14917 26516-26530 NN denotes immunostaining
T14918 26531-26533 IN denotes of
T14919 26534-26541 NNS denotes kidneys
T14920 26542-26543 -LRB- denotes (
T14921 26543-26549 NN denotes Figure
T14922 26550-26552 CD denotes 5A
T14923 26552-26553 -RRB- denotes )
T14924 26553-26554 . denotes .
T14925 26554-26716 sentence denotes The presence of 50% of the normal amount of wild-type protein may explain the lack of symptoms, but it cannot explain the lack of any ER-retained mutant protein.
T14926 26555-26558 DT denotes The
T14927 26559-26567 NN denotes presence
T14929 26568-26570 IN denotes of
T14930 26571-26573 CD denotes 50
T14931 26573-26574 NN denotes %
T14932 26575-26577 IN denotes of
T14933 26578-26581 DT denotes the
T14935 26582-26588 JJ denotes normal
T14934 26589-26595 NN denotes amount
T14936 26596-26598 IN denotes of
T14937 26599-26603 JJ denotes wild
T14939 26603-26604 HYPH denotes -
T14938 26604-26608 NN denotes type
T14940 26609-26616 NN denotes protein
T14941 26617-26620 MD denotes may
T14928 26621-26628 VB denotes explain
T14942 26629-26632 DT denotes the
T14943 26633-26637 NN denotes lack
T14944 26638-26640 IN denotes of
T14945 26641-26649 NNS denotes symptoms
T14946 26649-26651 , denotes ,
T14947 26651-26654 CC denotes but
T14948 26655-26657 PRP denotes it
T14950 26658-26661 MD denotes can
T14951 26661-26664 RB denotes not
T14949 26665-26672 VB denotes explain
T14952 26673-26676 DT denotes the
T14953 26677-26681 NN denotes lack
T14954 26682-26684 IN denotes of
T14955 26685-26688 DT denotes any
T14957 26689-26691 NN denotes ER
T14959 26691-26692 HYPH denotes -
T14958 26692-26700 VBN denotes retained
T14960 26701-26707 NN denotes mutant
T14956 26708-26715 NN denotes protein
T14961 26715-26716 . denotes .
T14962 26716-26841 sentence denotes Rather, the phenotype of the Aqp2F204V/+ animals suggests that the mutant protein is being rescued by the wild-type protein.
T14963 26717-26723 RB denotes Rather
T14965 26723-26725 , denotes ,
T14966 26725-26728 DT denotes the
T14967 26729-26738 NN denotes phenotype
T14968 26739-26741 IN denotes of
T14969 26742-26745 DT denotes the
T14971 26746-26755 NN denotes Aqp2F204V
T14972 26755-26756 HYPH denotes /
T14973 26756-26757 SYM denotes +
T14970 26758-26765 NNS denotes animals
T14964 26766-26774 VBZ denotes suggests
T14974 26775-26779 IN denotes that
T14976 26780-26783 DT denotes the
T14978 26784-26790 NN denotes mutant
T14977 26791-26798 NN denotes protein
T14979 26799-26801 VBZ denotes is
T14980 26802-26807 VBG denotes being
T14975 26808-26815 VBN denotes rescued
T14981 26816-26818 IN denotes by
T14982 26819-26822 DT denotes the
T14984 26823-26827 JJ denotes wild
T14986 26827-26828 HYPH denotes -
T14985 26828-26832 NN denotes type
T14983 26833-26840 NN denotes protein
T14987 26840-26841 . denotes .
T14988 26841-27071 sentence denotes Indeed, de Mattia et al. (26) have demonstrated that one recessive allele of Aqp2, P262L, does not properly translocate when expressed by itself in MDCK cells, but that in the presence of wild-type protein, it localizes normally.
T14989 26842-26848 RB denotes Indeed
T14991 26848-26850 , denotes ,
T14992 26850-26852 NNP denotes de
T14993 26853-26859 NNP denotes Mattia
T14994 26860-26862 FW denotes et
T14995 26863-26866 FW denotes al.
T14996 26867-26868 -LRB- denotes (
T14997 26868-26870 CD denotes 26
T14998 26870-26871 -RRB- denotes )
T14999 26872-26876 VBP denotes have
T14990 26877-26889 VBN denotes demonstrated
T15000 26890-26894 IN denotes that
T15002 26895-26898 CD denotes one
T15004 26899-26908 JJ denotes recessive
T15003 26909-26915 NN denotes allele
T15005 26916-26918 IN denotes of
T15006 26919-26923 NN denotes Aqp2
T15007 26923-26925 , denotes ,
T15008 26925-26930 NN denotes P262L
T15009 26930-26932 , denotes ,
T15010 26932-26936 VBZ denotes does
T15011 26937-26940 RB denotes not
T15012 26941-26949 RB denotes properly
T15001 26950-26961 VB denotes translocate
T15013 26962-26966 WRB denotes when
T15014 26967-26976 VBN denotes expressed
T15015 26977-26979 IN denotes by
T15016 26980-26986 PRP denotes itself
T15017 26987-26989 IN denotes in
T15018 26990-26994 NN denotes MDCK
T15019 26995-27000 NNS denotes cells
T15020 27000-27002 , denotes ,
T15021 27002-27005 CC denotes but
T15022 27006-27010 IN denotes that
T15024 27011-27013 IN denotes in
T15025 27014-27017 DT denotes the
T15026 27018-27026 NN denotes presence
T15027 27027-27029 IN denotes of
T15028 27030-27034 JJ denotes wild
T15030 27034-27035 HYPH denotes -
T15029 27035-27039 NN denotes type
T15031 27040-27047 NN denotes protein
T15032 27047-27049 , denotes ,
T15033 27049-27051 PRP denotes it
T15023 27052-27061 VBZ denotes localizes
T15034 27062-27070 RB denotes normally
T15035 27070-27071 . denotes .
T15036 27071-27136 sentence denotes The same mechanism seems to apply in vivo with Aqp2F204V/+ mice.
T15037 27072-27075 DT denotes The
T15039 27076-27080 JJ denotes same
T15038 27081-27090 NN denotes mechanism
T15040 27091-27096 VBZ denotes seems
T15041 27097-27099 TO denotes to
T15042 27100-27105 VB denotes apply
T15043 27106-27108 FW denotes in
T15044 27109-27113 FW denotes vivo
T15045 27114-27118 IN denotes with
T15046 27119-27128 NN denotes Aqp2F204V
T15048 27128-27129 HYPH denotes /
T15049 27129-27130 SYM denotes +
T15047 27131-27135 NNS denotes mice
T15050 27135-27136 . denotes .
T15051 27136-27335 sentence denotes In support of this, AQP2-F204V can interact with wild-type AQP2 (Figure 5B), and when coexpressed with wild-type protein, AQP2-F204V can reach the cell surface (Figure 5C bottom right panel and 5D).
T15052 27137-27139 IN denotes In
T15054 27140-27147 NN denotes support
T15055 27148-27150 IN denotes of
T15056 27151-27155 DT denotes this
T15057 27155-27157 , denotes ,
T15058 27157-27161 NN denotes AQP2
T15060 27161-27162 HYPH denotes -
T15059 27162-27167 NN denotes F204V
T15061 27168-27171 MD denotes can
T15053 27172-27180 VB denotes interact
T15062 27181-27185 IN denotes with
T15063 27186-27190 JJ denotes wild
T15065 27190-27191 HYPH denotes -
T15064 27191-27195 NN denotes type
T15066 27196-27200 NN denotes AQP2
T15067 27201-27202 -LRB- denotes (
T15068 27202-27208 NN denotes Figure
T15069 27209-27211 CD denotes 5B
T15070 27211-27212 -RRB- denotes )
T15071 27212-27214 , denotes ,
T15072 27214-27217 CC denotes and
T15073 27218-27222 WRB denotes when
T15074 27223-27234 VBN denotes coexpressed
T15076 27235-27239 IN denotes with
T15077 27240-27244 JJ denotes wild
T15079 27244-27245 HYPH denotes -
T15078 27245-27249 NN denotes type
T15080 27250-27257 NN denotes protein
T15081 27257-27259 , denotes ,
T15082 27259-27263 NN denotes AQP2
T15084 27263-27264 HYPH denotes -
T15083 27264-27269 NN denotes F204V
T15085 27270-27273 MD denotes can
T15075 27274-27279 VB denotes reach
T15086 27280-27283 DT denotes the
T15088 27284-27288 NN denotes cell
T15087 27289-27296 NN denotes surface
T15089 27297-27298 -LRB- denotes (
T15091 27298-27304 NN denotes Figure
T15092 27305-27307 CD denotes 5C
T15093 27308-27314 NN denotes bottom
T15094 27315-27320 JJ denotes right
T15090 27321-27326 NN denotes panel
T15095 27327-27330 CC denotes and
T15096 27331-27333 CD denotes 5D
T15097 27333-27334 -RRB- denotes )
T15098 27334-27335 . denotes .
T15099 27335-27637 sentence denotes Although it has been demonstrated that a recessive allele (encoding AQP2-R187C) of NDI fails to interact with wild-type AQP2 [6], here we show that AQP2-F204V does interact with the wild-type protein, presumably as part of heterotetramers, and represents a rescuable allele, both in vitro and in vivo.
T15100 27336-27344 IN denotes Although
T15102 27345-27347 PRP denotes it
T15103 27348-27351 VBZ denotes has
T15104 27352-27356 VBN denotes been
T15101 27357-27369 VBN denotes demonstrated
T15106 27370-27374 IN denotes that
T15108 27375-27376 DT denotes a
T15110 27377-27386 JJ denotes recessive
T15109 27387-27393 NN denotes allele
T15111 27394-27395 -LRB- denotes (
T15112 27395-27403 VBG denotes encoding
T15113 27404-27408 NN denotes AQP2
T15115 27408-27409 HYPH denotes -
T15114 27409-27414 NN denotes R187C
T15116 27414-27415 -RRB- denotes )
T15117 27416-27418 IN denotes of
T15118 27419-27422 NN denotes NDI
T15107 27423-27428 VBZ denotes fails
T15119 27429-27431 TO denotes to
T15120 27432-27440 VB denotes interact
T15121 27441-27445 IN denotes with
T15122 27446-27450 JJ denotes wild
T15124 27450-27451 HYPH denotes -
T15123 27451-27455 NN denotes type
T15125 27456-27460 NN denotes AQP2
T15126 27461-27462 -LRB- denotes [
T15127 27462-27463 CD denotes 6
T15128 27463-27464 -RRB- denotes ]
T15129 27464-27466 , denotes ,
T15130 27466-27470 RB denotes here
T15131 27471-27473 PRP denotes we
T15105 27474-27478 VBP denotes show
T15132 27479-27483 IN denotes that
T15134 27484-27488 NN denotes AQP2
T15136 27488-27489 HYPH denotes -
T15135 27489-27494 NN denotes F204V
T15137 27495-27499 VBZ denotes does
T15133 27500-27508 VB denotes interact
T15138 27509-27513 IN denotes with
T15139 27514-27517 DT denotes the
T15141 27518-27522 JJ denotes wild
T15143 27522-27523 HYPH denotes -
T15142 27523-27527 NN denotes type
T15140 27528-27535 NN denotes protein
T15144 27535-27537 , denotes ,
T15145 27537-27547 RB denotes presumably
T15146 27548-27550 IN denotes as
T15147 27551-27555 NN denotes part
T15148 27556-27558 IN denotes of
T15149 27559-27574 NNS denotes heterotetramers
T15150 27574-27576 , denotes ,
T15151 27576-27579 CC denotes and
T15152 27580-27590 VBZ denotes represents
T15153 27591-27592 DT denotes a
T15155 27593-27602 JJ denotes rescuable
T15154 27603-27609 NN denotes allele
T15156 27609-27611 , denotes ,
T15157 27611-27615 CC denotes both
T15159 27616-27618 FW denotes in
T15158 27619-27624 FW denotes vitro
T15160 27625-27628 CC denotes and
T15161 27629-27631 FW denotes in
T15162 27632-27636 FW denotes vivo
T15163 27636-27637 . denotes .
T15164 27637-27871 sentence denotes Immunostaining the kidneys of homozygous Aqp2F204V/F204V mice shows that the mutant-expressing collecting duct cells can not mediate water reabsorption, because it fails to insert into the apical plasma membrane in response to dDAVP.
T15165 27638-27652 VBG denotes Immunostaining
T15167 27653-27656 DT denotes the
T15168 27657-27664 NNS denotes kidneys
T15169 27665-27667 IN denotes of
T15170 27668-27678 JJ denotes homozygous
T15172 27679-27688 NN denotes Aqp2F204V
T15174 27688-27689 HYPH denotes /
T15173 27689-27694 NN denotes F204V
T15171 27695-27699 NNS denotes mice
T15166 27700-27705 VBZ denotes shows
T15175 27706-27710 IN denotes that
T15177 27711-27714 DT denotes the
T15179 27715-27721 NN denotes mutant
T15181 27721-27722 HYPH denotes -
T15180 27722-27732 VBG denotes expressing
T15182 27733-27743 VBG denotes collecting
T15183 27744-27748 NN denotes duct
T15178 27749-27754 NNS denotes cells
T15184 27755-27758 MD denotes can
T15185 27759-27762 RB denotes not
T15176 27763-27770 VB denotes mediate
T15186 27771-27776 NN denotes water
T15187 27777-27789 NN denotes reabsorption
T15188 27789-27791 , denotes ,
T15189 27791-27798 IN denotes because
T15191 27799-27801 PRP denotes it
T15190 27802-27807 VBZ denotes fails
T15192 27808-27810 TO denotes to
T15193 27811-27817 VB denotes insert
T15194 27818-27822 IN denotes into
T15195 27823-27826 DT denotes the
T15197 27827-27833 JJ denotes apical
T15198 27834-27840 NN denotes plasma
T15196 27841-27849 NN denotes membrane
T15199 27850-27852 IN denotes in
T15200 27853-27861 NN denotes response
T15201 27862-27864 IN denotes to
T15202 27865-27870 NN denotes dDAVP
T15203 27870-27871 . denotes .
T15204 27871-27998 sentence denotes This is this first in vivo proof of a long-standing hypothesis that comes from in vitro studies with recessive Aqp2 mutations.
T15205 27872-27876 DT denotes This
T15206 27877-27879 VBZ denotes is
T15207 27880-27884 DT denotes this
T15209 27885-27890 JJ denotes first
T15210 27891-27893 FW denotes in
T15211 27894-27898 FW denotes vivo
T15208 27899-27904 NN denotes proof
T15212 27905-27907 IN denotes of
T15213 27908-27909 DT denotes a
T15215 27910-27914 JJ denotes long
T15217 27914-27915 HYPH denotes -
T15216 27915-27923 VBG denotes standing
T15214 27924-27934 NN denotes hypothesis
T15218 27935-27939 WDT denotes that
T15219 27940-27945 VBZ denotes comes
T15220 27946-27950 IN denotes from
T15221 27951-27953 FW denotes in
T15222 27954-27959 FW denotes vitro
T15223 27960-27967 NNS denotes studies
T15224 27968-27972 IN denotes with
T15225 27973-27982 JJ denotes recessive
T15227 27983-27987 NN denotes Aqp2
T15226 27988-27997 NNS denotes mutations
T15228 27997-27998 . denotes .
T15229 27998-28217 sentence denotes Transfection into MDCK cells of any of several Aqp2 mutations corresponding to recessive human alleles shows abnormal subcellular localization [25], [27] and failure to appropriately translocate to the plasma membrane.
T15230 27999-28011 NN denotes Transfection
T15232 28012-28016 IN denotes into
T15233 28017-28021 NN denotes MDCK
T15234 28022-28027 NNS denotes cells
T15235 28028-28030 IN denotes of
T15236 28031-28034 DT denotes any
T15237 28035-28037 IN denotes of
T15238 28038-28045 JJ denotes several
T15240 28046-28050 NN denotes Aqp2
T15239 28051-28060 NNS denotes mutations
T15241 28061-28074 VBG denotes corresponding
T15242 28075-28077 IN denotes to
T15243 28078-28087 JJ denotes recessive
T15245 28088-28093 JJ denotes human
T15244 28094-28101 NNS denotes alleles
T15231 28102-28107 VBZ denotes shows
T15246 28108-28116 JJ denotes abnormal
T15248 28117-28128 JJ denotes subcellular
T15247 28129-28141 NN denotes localization
T15249 28142-28143 -LRB- denotes [
T15250 28143-28145 CD denotes 25
T15251 28145-28146 -RRB- denotes ]
T15252 28146-28148 , denotes ,
T15253 28148-28149 -LRB- denotes [
T15254 28149-28151 CD denotes 27
T15255 28151-28152 -RRB- denotes ]
T15256 28153-28156 CC denotes and
T15257 28157-28164 NN denotes failure
T15258 28165-28167 TO denotes to
T15260 28168-28181 RB denotes appropriately
T15259 28182-28193 VB denotes translocate
T15261 28194-28196 IN denotes to
T15262 28197-28200 DT denotes the
T15264 28201-28207 NN denotes plasma
T15263 28208-28216 NN denotes membrane
T15265 28216-28217 . denotes .
T15266 28217-28364 sentence denotes Thus, misfolding, retention in the ER, and failure to translocate in response to dDAVP were proposed as the mechanism for autosomal recessive NDI.
T15267 28218-28222 RB denotes Thus
T15269 28222-28224 , denotes ,
T15270 28224-28234 NN denotes misfolding
T15271 28234-28236 , denotes ,
T15272 28236-28245 NN denotes retention
T15273 28246-28248 IN denotes in
T15274 28249-28252 DT denotes the
T15275 28253-28255 NN denotes ER
T15276 28255-28257 , denotes ,
T15277 28257-28260 CC denotes and
T15278 28261-28268 NN denotes failure
T15279 28269-28271 TO denotes to
T15280 28272-28283 VB denotes translocate
T15281 28284-28286 IN denotes in
T15282 28287-28295 NN denotes response
T15283 28296-28298 IN denotes to
T15284 28299-28304 NN denotes dDAVP
T15285 28305-28309 VBD denotes were
T15268 28310-28318 VBN denotes proposed
T15286 28319-28321 IN denotes as
T15287 28322-28325 DT denotes the
T15288 28326-28335 NN denotes mechanism
T15289 28336-28339 IN denotes for
T15290 28340-28349 JJ denotes autosomal
T15292 28350-28359 JJ denotes recessive
T15291 28360-28363 NN denotes NDI
T15293 28363-28364 . denotes .
T15294 28364-28452 sentence denotes Here we not only prove this hypothesis but also establish a useful model for human NDI.
T15295 28365-28369 RB denotes Here
T15297 28370-28372 PRP denotes we
T15298 28373-28376 RB denotes not
T15299 28377-28381 RB denotes only
T15296 28382-28387 VB denotes prove
T15300 28388-28392 DT denotes this
T15301 28393-28403 NN denotes hypothesis
T15302 28404-28407 CC denotes but
T15303 28408-28412 RB denotes also
T15304 28413-28422 VB denotes establish
T15305 28423-28424 DT denotes a
T15307 28425-28431 JJ denotes useful
T15306 28432-28437 NN denotes model
T15308 28438-28441 IN denotes for
T15309 28442-28447 JJ denotes human
T15310 28448-28451 NN denotes NDI
T15311 28451-28452 . denotes .
T15312 28452-28638 sentence denotes This mouse model of NDI based on an Aqp2 allele that can be rescued provides the opportunity to test therapies, including gene therapy, that may promote proper subcellular localization.
T15313 28453-28457 DT denotes This
T15315 28458-28463 NN denotes mouse
T15314 28464-28469 NN denotes model
T15317 28470-28472 IN denotes of
T15318 28473-28476 NN denotes NDI
T15319 28477-28482 VBN denotes based
T15320 28483-28485 IN denotes on
T15321 28486-28488 DT denotes an
T15323 28489-28493 NN denotes Aqp2
T15322 28494-28500 NN denotes allele
T15324 28501-28505 WDT denotes that
T15326 28506-28509 MD denotes can
T15327 28510-28512 VB denotes be
T15325 28513-28520 VBN denotes rescued
T15316 28521-28529 VBZ denotes provides
T15328 28530-28533 DT denotes the
T15329 28534-28545 NN denotes opportunity
T15330 28546-28548 TO denotes to
T15331 28549-28553 VB denotes test
T15332 28554-28563 NNS denotes therapies
T15333 28563-28565 , denotes ,
T15334 28565-28574 VBG denotes including
T15335 28575-28579 NN denotes gene
T15336 28580-28587 NN denotes therapy
T15337 28587-28589 , denotes ,
T15338 28589-28593 WDT denotes that
T15340 28594-28597 MD denotes may
T15339 28598-28605 VB denotes promote
T15341 28606-28612 JJ denotes proper
T15343 28613-28624 JJ denotes subcellular
T15342 28625-28637 NN denotes localization
T15344 28637-28638 . denotes .
T15507 28663-28673 NN denotes Generation
T15508 28674-28676 IN denotes of
T15509 28677-28680 NN denotes ENU
T15510 28681-28685 NNS denotes mice
T15511 28686-28689 CC denotes and
T15512 28690-28697 NN denotes housing
T15513 28697-28698 . denotes .
T15514 28698-28761 sentence denotes ENU mutagenized C57BL/6 mice were generated as described [19].
T15515 28699-28702 NN denotes ENU
T15517 28703-28714 VBN denotes mutagenized
T15518 28715-28720 NN denotes C57BL
T15519 28720-28721 HYPH denotes /
T15520 28721-28722 CD denotes 6
T15516 28723-28727 NNS denotes mice
T15522 28728-28732 VBD denotes were
T15521 28733-28742 VBN denotes generated
T15523 28743-28745 IN denotes as
T15524 28746-28755 VBN denotes described
T15525 28756-28757 -LRB- denotes [
T15526 28757-28759 CD denotes 19
T15527 28759-28760 -RRB- denotes ]
T15528 28760-28761 . denotes .
T15529 28761-28977 sentence denotes Mice were maintained by backcrossing affected animals to C57BL/6 and housed in the Genomics Institute of the Novartis Research Foundation Specific Pathogen Free animal facility (La Jolla, California, United States).
T15530 28762-28766 NNS denotes Mice
T15532 28767-28771 VBD denotes were
T15531 28772-28782 VBN denotes maintained
T15533 28783-28785 IN denotes by
T15534 28786-28798 VBG denotes backcrossing
T15535 28799-28807 VBN denotes affected
T15536 28808-28815 NNS denotes animals
T15537 28816-28818 IN denotes to
T15538 28819-28824 NN denotes C57BL
T15539 28824-28825 HYPH denotes /
T15540 28825-28826 CD denotes 6
T15541 28827-28830 CC denotes and
T15542 28831-28837 VBN denotes housed
T15543 28838-28840 IN denotes in
T15544 28841-28844 DT denotes the
T15546 28845-28853 NNP denotes Genomics
T15545 28854-28863 NNP denotes Institute
T15547 28864-28866 IN denotes of
T15548 28867-28870 DT denotes the
T15550 28871-28879 NNP denotes Novartis
T15552 28880-28888 NNP denotes Research
T15551 28889-28899 NNP denotes Foundation
T15553 28900-28908 NNP denotes Specific
T15554 28909-28917 NNP denotes Pathogen
T15555 28918-28922 NNP denotes Free
T15556 28923-28929 NNP denotes animal
T15549 28930-28938 NNP denotes facility
T15557 28939-28940 -LRB- denotes (
T15559 28940-28942 NNP denotes La
T15558 28943-28948 NNP denotes Jolla
T15560 28948-28950 , denotes ,
T15561 28950-28960 NNP denotes California
T15562 28960-28962 , denotes ,
T15563 28962-28968 NNP denotes United
T15564 28969-28975 NNP denotes States
T15565 28975-28976 -RRB- denotes )
T15566 28976-28977 . denotes .
T15567 28977-29113 sentence denotes All procedures were approved by the Genomics Institute of the Novartis Research Foundation Institutional Animal Care and Use Committee.
T15568 28978-28981 DT denotes All
T15569 28982-28992 NNS denotes procedures
T15571 28993-28997 VBD denotes were
T15570 28998-29006 VBN denotes approved
T15572 29007-29009 IN denotes by
T15573 29010-29013 DT denotes the
T15575 29014-29022 NNP denotes Genomics
T15574 29023-29032 NNP denotes Institute
T15576 29033-29035 IN denotes of
T15577 29036-29039 DT denotes the
T15579 29040-29048 NNP denotes Novartis
T15581 29049-29057 NNP denotes Research
T15580 29058-29068 NNP denotes Foundation
T15582 29069-29082 NNP denotes Institutional
T15583 29083-29089 NNP denotes Animal
T15584 29090-29094 NNP denotes Care
T15585 29095-29098 CC denotes and
T15586 29099-29102 NNP denotes Use
T15578 29103-29112 NNP denotes Committee
T15587 29112-29113 . denotes .
T15900 29115-29125 NNS denotes Constructs
T15901 29125-29126 . denotes .
T15902 29126-29328 sentence denotes The complete coding sequence of mouse AQP2 from an IMAGE clone was digested from the pCMV⋅SPORT6 plasmid with EcoRI and NotI and ligated into pcDNA3.1 (Invitrogen, Carlsbad, California, United States).
T15903 29127-29130 DT denotes The
T15905 29131-29139 JJ denotes complete
T15906 29140-29146 NN denotes coding
T15904 29147-29155 NN denotes sequence
T15908 29156-29158 IN denotes of
T15909 29159-29164 NN denotes mouse
T15910 29165-29169 NN denotes AQP2
T15911 29170-29174 IN denotes from
T15912 29175-29177 DT denotes an
T15914 29178-29183 NN denotes IMAGE
T15913 29184-29189 NN denotes clone
T15915 29190-29193 VBD denotes was
T15907 29194-29202 VBN denotes digested
T15916 29203-29207 IN denotes from
T15917 29208-29211 DT denotes the
T15919 29212-29223 NN denotes pCMV⋅SPORT6
T15918 29224-29231 NN denotes plasmid
T15920 29232-29236 IN denotes with
T15921 29237-29242 NN denotes EcoRI
T15922 29243-29246 CC denotes and
T15923 29247-29251 NN denotes NotI
T15924 29252-29255 CC denotes and
T15925 29256-29263 VBN denotes ligated
T15926 29264-29268 IN denotes into
T15927 29269-29277 NN denotes pcDNA3.1
T15928 29278-29279 -LRB- denotes (
T15929 29279-29289 NNP denotes Invitrogen
T15930 29289-29291 , denotes ,
T15931 29291-29299 NNP denotes Carlsbad
T15932 29299-29301 , denotes ,
T15933 29301-29311 NNP denotes California
T15934 29311-29313 , denotes ,
T15935 29313-29319 NNP denotes United
T15936 29320-29326 NNP denotes States
T15937 29326-29327 -RRB- denotes )
T15938 29327-29328 . denotes .
T15939 29328-29582 sentence denotes The F204V mutation was introduced by site-directed mutagenesis (Stratagene, La Jolla, California, United States), using the sense oligonucleotide 5′-GATGATCACTGGGTCGTCTGGATCGGACCCC-3′, and antisense oligonucleotide 5′-GGGGTCCGATCCAGACGACCCAGTGATCATC-3′.
T15940 29329-29332 DT denotes The
T15942 29333-29338 NN denotes F204V
T15941 29339-29347 NN denotes mutation
T15944 29348-29351 VBD denotes was
T15943 29352-29362 VBN denotes introduced
T15945 29363-29365 IN denotes by
T15946 29366-29370 NN denotes site
T15948 29370-29371 HYPH denotes -
T15947 29371-29379 JJ denotes directed
T15949 29380-29391 NN denotes mutagenesis
T15950 29392-29393 -LRB- denotes (
T15951 29393-29403 NNP denotes Stratagene
T15952 29403-29405 , denotes ,
T15953 29405-29407 NNP denotes La
T15954 29408-29413 NNP denotes Jolla
T15955 29413-29415 , denotes ,
T15956 29415-29425 NNP denotes California
T15957 29425-29427 , denotes ,
T15958 29427-29433 NNP denotes United
T15959 29434-29440 NNP denotes States
T15960 29440-29441 -RRB- denotes )
T15961 29441-29443 , denotes ,
T15962 29443-29448 VBG denotes using
T15963 29449-29452 DT denotes the
T15965 29453-29458 NN denotes sense
T15964 29459-29474 NN denotes oligonucleotide
T15966 29475-29476 CD denotes 5
T15968 29476-29477 SYM denotes ′
T15969 29477-29478 HYPH denotes -
T15967 29478-29509 NN denotes GATGATCACTGGGTCGTCTGGATCGGACCCC
T15970 29509-29510 HYPH denotes -
T15971 29510-29511 CD denotes 3
T15972 29511-29512 SYM denotes ′
T15973 29512-29514 , denotes ,
T15974 29514-29517 CC denotes and
T15975 29518-29527 JJ denotes antisense
T15976 29528-29543 NN denotes oligonucleotide
T15977 29544-29545 CD denotes 5
T15979 29545-29546 SYM denotes ′
T15980 29546-29547 HYPH denotes -
T15978 29547-29578 NN denotes GGGGTCCGATCCAGACGACCCAGTGATCATC
T15981 29578-29579 HYPH denotes -
T15982 29579-29580 CD denotes 3
T15983 29580-29581 SYM denotes ′
T15984 29581-29582 . denotes .
T15985 29582-29771 sentence denotes To generate GFP fusions of AQP2, the pCMV⋅SPORT6 AQP2 construct was used in a PCR reaction with the primers Sp6 and 5′-GACTGGATCCCGGCCTTGCTGCCGCGCGGCAG-3′ to remove the stop codon of AQP2.
T15986 29583-29585 TO denotes To
T15987 29586-29594 VB denotes generate
T15989 29595-29598 NN denotes GFP
T15990 29599-29606 NNS denotes fusions
T15991 29607-29609 IN denotes of
T15992 29610-29614 NN denotes AQP2
T15993 29614-29616 , denotes ,
T15994 29616-29619 DT denotes the
T15996 29620-29631 NN denotes pCMV⋅SPORT6
T15997 29632-29636 NN denotes AQP2
T15995 29637-29646 NN denotes construct
T15998 29647-29650 VBD denotes was
T15988 29651-29655 VBN denotes used
T15999 29656-29658 IN denotes in
T16000 29659-29660 DT denotes a
T16002 29661-29664 NN denotes PCR
T16001 29665-29673 NN denotes reaction
T16003 29674-29678 IN denotes with
T16004 29679-29682 DT denotes the
T16006 29683-29690 NNS denotes primers
T16005 29691-29694 NN denotes Sp6
T16007 29695-29698 CC denotes and
T16008 29699-29700 CD denotes 5
T16010 29700-29701 SYM denotes ′
T16011 29701-29702 HYPH denotes -
T16009 29702-29734 NN denotes GACTGGATCCCGGCCTTGCTGCCGCGCGGCAG
T16012 29734-29735 HYPH denotes -
T16013 29735-29736 CD denotes 3
T16014 29736-29737 SYM denotes ′
T16015 29738-29740 TO denotes to
T16016 29741-29747 VB denotes remove
T16017 29748-29751 DT denotes the
T16019 29752-29756 NN denotes stop
T16018 29757-29762 NN denotes codon
T16020 29763-29765 IN denotes of
T16021 29766-29770 NN denotes AQP2
T16022 29770-29771 . denotes .
T16023 29771-29898 sentence denotes The product was digested with KpnI and BamHI and ligated into pEGFP-N2 (BD Biosciences, San Diego, California, United States).
T16024 29772-29775 DT denotes The
T16025 29776-29783 NN denotes product
T16027 29784-29787 VBD denotes was
T16026 29788-29796 VBN denotes digested
T16028 29797-29801 IN denotes with
T16029 29802-29806 NN denotes KpnI
T16030 29807-29810 CC denotes and
T16031 29811-29816 NN denotes BamHI
T16032 29817-29820 CC denotes and
T16033 29821-29828 VBN denotes ligated
T16034 29829-29833 IN denotes into
T16035 29834-29839 NN denotes pEGFP
T16037 29839-29840 HYPH denotes -
T16036 29840-29842 NN denotes N2
T16038 29843-29844 -LRB- denotes (
T16040 29844-29846 NN denotes BD
T16039 29847-29858 NNP denotes Biosciences
T16041 29858-29860 , denotes ,
T16042 29860-29863 NNP denotes San
T16043 29864-29869 NNP denotes Diego
T16044 29869-29871 , denotes ,
T16045 29871-29881 NNP denotes California
T16046 29881-29883 , denotes ,
T16047 29883-29889 NNP denotes United
T16048 29890-29896 NNP denotes States
T16049 29896-29897 -RRB- denotes )
T16050 29897-29898 . denotes .
T16051 29898-29975 sentence denotes The F204V mutation was introduced using the same mutagenic oligonucleotides.
T16052 29899-29902 DT denotes The
T16054 29903-29908 NN denotes F204V
T16053 29909-29917 NN denotes mutation
T16056 29918-29921 VBD denotes was
T16055 29922-29932 VBN denotes introduced
T16057 29933-29938 VBG denotes using
T16058 29939-29942 DT denotes the
T16060 29943-29947 JJ denotes same
T16061 29948-29957 JJ denotes mutagenic
T16059 29958-29974 NNS denotes oligonucleotides
T16062 29974-29975 . denotes .
T16399 29977-29981 NN denotes Cell
T16400 29982-29989 NN denotes culture
T16401 29990-29993 CC denotes and
T16402 29994-30004 NN denotes generation
T16403 30005-30007 IN denotes of
T16404 30008-30014 JJ denotes stable
T16406 30015-30019 NN denotes cell
T16405 30020-30025 NNS denotes lines
T16407 30025-30026 . denotes .
T16408 30026-30278 sentence denotes MDCK cells (CCL-34; ATCC, Manassas, Virginia, United States) were cultured in DMEM (Sigma-Aldrich, St. Louis, Missouri, United States) supplemented with 10% FBS (Sigma-Aldrich), 100 U/ml of penicillin, and 100 μg/ml of streptomycin at 37 °C in 5% CO2.
T16409 30027-30031 NN denotes MDCK
T16410 30032-30037 NNS denotes cells
T16412 30038-30039 -LRB- denotes (
T16414 30039-30042 NN denotes CCL
T16415 30042-30043 HYPH denotes -
T16416 30043-30045 CD denotes 34
T16417 30045-30046 : denotes ;
T16413 30047-30051 NN denotes ATCC
T16418 30051-30053 , denotes ,
T16419 30053-30061 NNP denotes Manassas
T16420 30061-30063 , denotes ,
T16421 30063-30071 NNP denotes Virginia
T16422 30071-30073 , denotes ,
T16423 30073-30079 NNP denotes United
T16424 30080-30086 NNP denotes States
T16425 30086-30087 -RRB- denotes )
T16426 30088-30092 VBD denotes were
T16411 30093-30101 VBN denotes cultured
T16427 30102-30104 IN denotes in
T16428 30105-30109 NN denotes DMEM
T16429 30110-30111 -LRB- denotes (
T16431 30111-30116 NNP denotes Sigma
T16432 30116-30117 HYPH denotes -
T16430 30117-30124 NNP denotes Aldrich
T16433 30124-30126 , denotes ,
T16434 30126-30129 NNP denotes St.
T16435 30130-30135 NNP denotes Louis
T16436 30135-30137 , denotes ,
T16437 30137-30145 NNP denotes Missouri
T16438 30145-30147 , denotes ,
T16439 30147-30153 NNP denotes United
T16440 30154-30160 NNP denotes States
T16441 30160-30161 -RRB- denotes )
T16442 30162-30174 VBN denotes supplemented
T16443 30175-30179 IN denotes with
T16444 30180-30182 CD denotes 10
T16445 30182-30183 NN denotes %
T16446 30184-30187 NN denotes FBS
T16447 30188-30189 -LRB- denotes (
T16449 30189-30194 NNP denotes Sigma
T16450 30194-30195 HYPH denotes -
T16448 30195-30202 NNP denotes Aldrich
T16451 30202-30203 -RRB- denotes )
T16452 30203-30205 , denotes ,
T16453 30205-30208 CD denotes 100
T16454 30209-30210 NN denotes U
T16455 30210-30211 SYM denotes /
T16456 30211-30213 NN denotes ml
T16457 30214-30216 IN denotes of
T16458 30217-30227 NN denotes penicillin
T16459 30227-30229 , denotes ,
T16460 30229-30232 CC denotes and
T16461 30233-30236 CD denotes 100
T16462 30237-30239 NN denotes μg
T16463 30239-30240 SYM denotes /
T16464 30240-30242 NN denotes ml
T16465 30243-30245 IN denotes of
T16466 30246-30258 NN denotes streptomycin
T16467 30259-30261 IN denotes at
T16468 30262-30264 CD denotes 37
T16469 30265-30267 NN denotes °C
T16470 30268-30270 IN denotes in
T16471 30271-30272 CD denotes 5
T16472 30272-30273 NN denotes %
T16473 30274-30277 NN denotes CO2
T16474 30277-30278 . denotes .
T16475 30278-30518 sentence denotes To generate stable MDCK cell lines, cells were transfected using Lipofectamine 2000 (Invitrogen) and the pcDNA3.1 expression constructs (containing wild-type AQP2, AQP2-F204V, or no insert) and selected with 900 μg/ml G418 (Sigma-Aldrich).
T16476 30279-30281 TO denotes To
T16477 30282-30290 VB denotes generate
T16479 30291-30297 JJ denotes stable
T16481 30298-30302 NN denotes MDCK
T16482 30303-30307 NN denotes cell
T16480 30308-30313 NNS denotes lines
T16483 30313-30315 , denotes ,
T16484 30315-30320 NNS denotes cells
T16485 30321-30325 VBD denotes were
T16478 30326-30337 VBN denotes transfected
T16486 30338-30343 VBG denotes using
T16487 30344-30357 NN denotes Lipofectamine
T16488 30358-30362 CD denotes 2000
T16489 30363-30364 -LRB- denotes (
T16490 30364-30374 NNP denotes Invitrogen
T16491 30374-30375 -RRB- denotes )
T16492 30376-30379 CC denotes and
T16493 30380-30383 DT denotes the
T16495 30384-30392 NN denotes pcDNA3.1
T16496 30393-30403 NN denotes expression
T16494 30404-30414 NNS denotes constructs
T16497 30415-30416 -LRB- denotes (
T16498 30416-30426 VBG denotes containing
T16499 30427-30431 JJ denotes wild
T16501 30431-30432 HYPH denotes -
T16500 30432-30436 NN denotes type
T16502 30437-30441 NN denotes AQP2
T16503 30441-30443 , denotes ,
T16504 30443-30447 NN denotes AQP2
T16506 30447-30448 HYPH denotes -
T16505 30448-30453 NN denotes F204V
T16507 30453-30455 , denotes ,
T16508 30455-30457 CC denotes or
T16509 30458-30460 DT denotes no
T16510 30461-30467 NN denotes insert
T16511 30467-30468 -RRB- denotes )
T16512 30469-30472 CC denotes and
T16513 30473-30481 VBN denotes selected
T16514 30482-30486 IN denotes with
T16515 30487-30490 CD denotes 900
T16516 30491-30493 NN denotes μg
T16518 30493-30494 SYM denotes /
T16519 30494-30496 NN denotes ml
T16517 30497-30501 NN denotes G418
T16520 30502-30503 -LRB- denotes (
T16522 30503-30508 NNP denotes Sigma
T16523 30508-30509 HYPH denotes -
T16521 30509-30516 NNP denotes Aldrich
T16524 30516-30517 -RRB- denotes )
T16525 30517-30518 . denotes .
T16526 30518-30564 sentence denotes Individual colonies were expanded 14 d later.
T16527 30519-30529 JJ denotes Individual
T16528 30530-30538 NNS denotes colonies
T16530 30539-30543 VBD denotes were
T16529 30544-30552 VBN denotes expanded
T16531 30553-30555 CD denotes 14
T16532 30556-30557 NN denotes d
T16533 30558-30563 RB denotes later
T16534 30563-30564 . denotes .
T16535 30564-30654 sentence denotes For the duration of these experiments, the antibiotic was continually added to the media.
T16536 30565-30568 IN denotes For
T16538 30569-30572 DT denotes the
T16539 30573-30581 NN denotes duration
T16540 30582-30584 IN denotes of
T16541 30585-30590 DT denotes these
T16542 30591-30602 NNS denotes experiments
T16543 30602-30604 , denotes ,
T16544 30604-30607 DT denotes the
T16545 30608-30618 NN denotes antibiotic
T16546 30619-30622 VBD denotes was
T16547 30623-30634 RB denotes continually
T16537 30635-30640 VBN denotes added
T16548 30641-30643 IN denotes to
T16549 30644-30647 DT denotes the
T16550 30648-30653 NNS denotes media
T16551 30653-30654 . denotes .
T16552 30654-30765 sentence denotes Transient GFP transfections were carried out in subconfluent stable cells lines also using Lipofectamine 2000.
T16553 30655-30664 JJ denotes Transient
T16555 30665-30668 NN denotes GFP
T16554 30669-30682 NNS denotes transfections
T16557 30683-30687 VBD denotes were
T16556 30688-30695 VBN denotes carried
T16558 30696-30699 RP denotes out
T16559 30700-30702 IN denotes in
T16560 30703-30715 JJ denotes subconfluent
T16562 30716-30722 JJ denotes stable
T16563 30723-30728 NNS denotes cells
T16561 30729-30734 NNS denotes lines
T16564 30735-30739 RB denotes also
T16565 30740-30745 VBG denotes using
T16566 30746-30759 NN denotes Lipofectamine
T16567 30760-30764 CD denotes 2000
T16568 30764-30765 . denotes .
T16676 30767-30777 NN denotes Sequencing
T16677 30778-30780 IN denotes of
T16678 30781-30785 NN denotes Aqp2
T16679 30786-30789 CC denotes and
T16680 30790-30800 NN denotes genotyping
T16681 30801-30803 IN denotes of
T16682 30804-30808 NNS denotes mice
T16683 30808-30809 . denotes .
T16684 30809-30880 sentence denotes All exons of Aqp2 were amplified from mouse genomic DNA and sequenced.
T16685 30810-30813 DT denotes All
T16686 30814-30819 NNS denotes exons
T16688 30820-30822 IN denotes of
T16689 30823-30827 NN denotes Aqp2
T16690 30828-30832 VBD denotes were
T16687 30833-30842 VBN denotes amplified
T16691 30843-30847 IN denotes from
T16692 30848-30853 NN denotes mouse
T16694 30854-30861 JJ denotes genomic
T16693 30862-30865 NN denotes DNA
T16695 30866-30869 CC denotes and
T16696 30870-30879 VBN denotes sequenced
T16697 30879-30880 . denotes .
T16698 30880-30994 sentence denotes For genotyping, exon 4 was amplified using the primers 5′-TCAGAACTTGCCCACTAGCC-3′ and 5′-TGTAGAGGAGGGAACCGATG-3′.
T16699 30881-30884 IN denotes For
T16701 30885-30895 NN denotes genotyping
T16702 30895-30897 , denotes ,
T16703 30897-30901 NN denotes exon
T16704 30902-30903 CD denotes 4
T16705 30904-30907 VBD denotes was
T16700 30908-30917 VBN denotes amplified
T16706 30918-30923 VBG denotes using
T16707 30924-30927 DT denotes the
T16708 30928-30935 NNS denotes primers
T16709 30936-30937 CD denotes 5
T16711 30937-30938 SYM denotes ′
T16712 30938-30939 HYPH denotes -
T16710 30939-30959 NN denotes TCAGAACTTGCCCACTAGCC
T16713 30959-30960 HYPH denotes -
T16714 30960-30961 CD denotes 3
T16715 30961-30962 SYM denotes ′
T16716 30963-30966 CC denotes and
T16717 30967-30968 CD denotes 5
T16719 30968-30969 SYM denotes ′
T16720 30969-30970 HYPH denotes -
T16718 30970-30990 NN denotes TGTAGAGGAGGGAACCGATG
T16721 30990-30991 HYPH denotes -
T16722 30991-30992 CD denotes 3
T16723 30992-30993 SYM denotes ′
T16724 30993-30994 . denotes .
T16959 30996-31001 NN denotes Urine
T16960 31002-31014 NNS denotes measurements
T16961 31014-31015 . denotes .
T16962 31015-31199 sentence denotes Total urine output was measured by separately housing adult mice in Nalgene Metabolic Cages (Minimitter, Bend, Oregon, United States) for 2–3 d and collecting urine every 24 h period.
T16963 31016-31021 JJ denotes Total
T16965 31022-31027 NN denotes urine
T16964 31028-31034 NN denotes output
T16967 31035-31038 VBD denotes was
T16966 31039-31047 VBN denotes measured
T16968 31048-31050 IN denotes by
T16969 31051-31061 RB denotes separately
T16970 31062-31069 VBG denotes housing
T16971 31070-31075 JJ denotes adult
T16972 31076-31080 NNS denotes mice
T16973 31081-31083 IN denotes in
T16974 31084-31091 NNP denotes Nalgene
T16976 31092-31101 JJ denotes Metabolic
T16975 31102-31107 NNS denotes Cages
T16977 31108-31109 -LRB- denotes (
T16978 31109-31119 NNP denotes Minimitter
T16979 31119-31121 , denotes ,
T16980 31121-31125 NNP denotes Bend
T16981 31125-31127 , denotes ,
T16982 31127-31133 NNP denotes Oregon
T16983 31133-31135 , denotes ,
T16984 31135-31141 NNP denotes United
T16985 31142-31148 NNP denotes States
T16986 31148-31149 -RRB- denotes )
T16987 31150-31153 IN denotes for
T16988 31154-31155 CD denotes 2
T16990 31155-31156 SYM denotes –
T16989 31156-31157 CD denotes 3
T16991 31158-31159 NN denotes d
T16992 31160-31163 CC denotes and
T16993 31164-31174 VBG denotes collecting
T16994 31175-31180 NN denotes urine
T16995 31181-31186 DT denotes every
T16997 31187-31189 CD denotes 24
T16998 31190-31191 NN denotes h
T16996 31192-31198 NN denotes period
T16999 31198-31199 . denotes .
T17000 31199-31326 sentence denotes Urine osmolalities were determined using an Osmometer (Osmette 5004; Precision Systems, Natick, Massachusetts, United States).
T17001 31200-31205 NN denotes Urine
T17002 31206-31218 NNS denotes osmolalities
T17004 31219-31223 VBD denotes were
T17003 31224-31234 VBN denotes determined
T17005 31235-31240 VBG denotes using
T17006 31241-31243 DT denotes an
T17007 31244-31253 NN denotes Osmometer
T17008 31254-31255 -LRB- denotes (
T17010 31255-31262 NNP denotes Osmette
T17011 31263-31267 CD denotes 5004
T17012 31267-31268 : denotes ;
T17013 31269-31278 NNP denotes Precision
T17009 31279-31286 NNP denotes Systems
T17014 31286-31288 , denotes ,
T17015 31288-31294 NNP denotes Natick
T17016 31294-31296 , denotes ,
T17017 31296-31309 NNP denotes Massachusetts
T17018 31309-31311 , denotes ,
T17019 31311-31317 NNP denotes United
T17020 31318-31324 NNP denotes States
T17021 31324-31325 -RRB- denotes )
T17022 31325-31326 . denotes .
T17023 31326-31426 sentence denotes Urine concentrating experiments were carried out by intraperitoneal injection of dDAVP (0.4 μg/kg).
T17024 31327-31332 NN denotes Urine
T17025 31333-31346 VBG denotes concentrating
T17026 31347-31358 NNS denotes experiments
T17028 31359-31363 VBD denotes were
T17027 31364-31371 VBN denotes carried
T17029 31372-31375 RP denotes out
T17030 31376-31378 IN denotes by
T17031 31379-31394 JJ denotes intraperitoneal
T17032 31395-31404 NN denotes injection
T17033 31405-31407 IN denotes of
T17034 31408-31413 NN denotes dDAVP
T17035 31414-31415 -LRB- denotes (
T17037 31415-31418 CD denotes 0.4
T17036 31419-31421 NN denotes μg
T17038 31421-31422 SYM denotes /
T17039 31422-31424 NN denotes kg
T17040 31424-31425 -RRB- denotes )
T17041 31425-31426 . denotes .
T17042 31426-31499 sentence denotes Mice were injected twice with dDAVP, once at time 0 and again at 30 min.
T17043 31427-31431 NNS denotes Mice
T17045 31432-31436 VBD denotes were
T17044 31437-31445 VBN denotes injected
T17046 31446-31451 RB denotes twice
T17047 31452-31456 IN denotes with
T17048 31457-31462 NN denotes dDAVP
T17049 31462-31464 , denotes ,
T17050 31464-31468 RB denotes once
T17051 31469-31471 IN denotes at
T17052 31472-31476 NN denotes time
T17053 31477-31478 CD denotes 0
T17054 31479-31482 CC denotes and
T17055 31483-31488 RB denotes again
T17056 31489-31491 IN denotes at
T17057 31492-31494 CD denotes 30
T17058 31495-31498 NN denotes min
T17059 31498-31499 . denotes .
T17060 31499-31589 sentence denotes Urine was collected at the start of the experiment and 30 min after the second injection.
T17061 31500-31505 NN denotes Urine
T17063 31506-31509 VBD denotes was
T17062 31510-31519 VBN denotes collected
T17064 31520-31522 IN denotes at
T17065 31523-31526 DT denotes the
T17066 31527-31532 NN denotes start
T17067 31533-31535 IN denotes of
T17068 31536-31539 DT denotes the
T17069 31540-31550 NN denotes experiment
T17070 31551-31554 CC denotes and
T17071 31555-31557 CD denotes 30
T17072 31558-31561 NN denotes min
T17073 31562-31567 IN denotes after
T17074 31568-31571 DT denotes the
T17076 31572-31578 JJ denotes second
T17075 31579-31588 NN denotes injection
T17077 31588-31589 . denotes .
T17265 31591-31597 NN denotes Kidney
T17266 31598-31606 NN denotes membrane
T17267 31607-31618 NN denotes preparation
T17268 31618-31619 . denotes .
T17269 31619-31800 sentence denotes Whole mouse kidneys were homogenized in 10 mM Tris (pH 7.4), 350 mM sucrose, and 5 mM EDTA containing protease inhibitors (Sigma-Aldrich, #P-8340) in a Potter-Elvehjem homogenizer.
T17270 31620-31625 JJ denotes Whole
T17272 31626-31631 NN denotes mouse
T17271 31632-31639 NNS denotes kidneys
T17274 31640-31644 VBD denotes were
T17273 31645-31656 VBN denotes homogenized
T17275 31657-31659 IN denotes in
T17276 31660-31662 CD denotes 10
T17277 31663-31665 NN denotes mM
T17278 31666-31670 NN denotes Tris
T17279 31671-31672 -LRB- denotes (
T17280 31672-31674 NN denotes pH
T17281 31675-31678 CD denotes 7.4
T17282 31678-31679 -RRB- denotes )
T17283 31679-31681 , denotes ,
T17284 31681-31684 CD denotes 350
T17285 31685-31687 NN denotes mM
T17286 31688-31695 NN denotes sucrose
T17287 31695-31697 , denotes ,
T17288 31697-31700 CC denotes and
T17289 31701-31702 CD denotes 5
T17290 31703-31705 NN denotes mM
T17291 31706-31710 NN denotes EDTA
T17292 31711-31721 VBG denotes containing
T17293 31722-31730 NN denotes protease
T17294 31731-31741 NNS denotes inhibitors
T17295 31742-31743 -LRB- denotes (
T17297 31743-31748 NNP denotes Sigma
T17299 31748-31749 HYPH denotes -
T17298 31749-31756 NNP denotes Aldrich
T17300 31756-31758 , denotes ,
T17301 31758-31759 SYM denotes #
T17296 31759-31760 NN denotes P
T17302 31760-31761 HYPH denotes -
T17303 31761-31765 CD denotes 8340
T17304 31765-31766 -RRB- denotes )
T17305 31767-31769 IN denotes in
T17306 31770-31771 DT denotes a
T17308 31772-31778 NNP denotes Potter
T17310 31778-31779 HYPH denotes -
T17309 31779-31787 NNP denotes Elvehjem
T17307 31788-31799 NN denotes homogenizer
T17311 31799-31800 . denotes .
T17312 31800-31940 sentence denotes The homogenate was centrifuged at 2,000 g for 10 min and the supernatant was subjected to ultracentrifugation at 100,000 g for 1 h at 4 °C.
T17313 31801-31804 DT denotes The
T17314 31805-31815 NN denotes homogenate
T17316 31816-31819 VBD denotes was
T17315 31820-31831 VBN denotes centrifuged
T17317 31832-31834 IN denotes at
T17318 31835-31840 CD denotes 2,000
T17319 31841-31842 NN denotes g
T17320 31843-31846 IN denotes for
T17321 31847-31849 CD denotes 10
T17322 31850-31853 NN denotes min
T17323 31854-31857 CC denotes and
T17324 31858-31861 DT denotes the
T17325 31862-31873 NN denotes supernatant
T17327 31874-31877 VBD denotes was
T17326 31878-31887 VBN denotes subjected
T17328 31888-31890 IN denotes to
T17329 31891-31910 NN denotes ultracentrifugation
T17330 31911-31913 IN denotes at
T17331 31914-31921 CD denotes 100,000
T17332 31922-31923 NN denotes g
T17333 31924-31927 IN denotes for
T17334 31928-31929 CD denotes 1
T17335 31930-31931 NN denotes h
T17336 31932-31934 IN denotes at
T17337 31935-31936 CD denotes 4
T17338 31937-31939 NN denotes °C
T17339 31939-31940 . denotes .
T17340 31940-32056 sentence denotes Pelleted membranes were resuspended in the same buffer, and protein concentration was determined by Bradford assay.
T17341 31941-31949 VBN denotes Pelleted
T17342 31950-31959 NNS denotes membranes
T17344 31960-31964 VBD denotes were
T17343 31965-31976 VBN denotes resuspended
T17345 31977-31979 IN denotes in
T17346 31980-31983 DT denotes the
T17348 31984-31988 JJ denotes same
T17347 31989-31995 NN denotes buffer
T17349 31995-31997 , denotes ,
T17350 31997-32000 CC denotes and
T17351 32001-32008 NN denotes protein
T17352 32009-32022 NN denotes concentration
T17354 32023-32026 VBD denotes was
T17353 32027-32037 VBN denotes determined
T17355 32038-32040 IN denotes by
T17356 32041-32049 NNP denotes Bradford
T17357 32050-32055 NN denotes assay
T17358 32055-32056 . denotes .
T17614 32058-32072 NN denotes Immunoblotting
T17615 32072-32073 . denotes .
T17616 32073-32199 sentence denotes Kidney membrane fractions (60 μg) were resolved on a 12% SDS-polyacrylamide gel and transferred to a nitrocellulose membrane.
T17617 32074-32080 NN denotes Kidney
T17618 32081-32089 NN denotes membrane
T17619 32090-32099 NNS denotes fractions
T17621 32100-32101 -LRB- denotes (
T17623 32101-32103 CD denotes 60
T17622 32104-32106 NN denotes μg
T17624 32106-32107 -RRB- denotes )
T17625 32108-32112 VBD denotes were
T17620 32113-32121 VBN denotes resolved
T17626 32122-32124 IN denotes on
T17627 32125-32126 DT denotes a
T17629 32127-32129 CD denotes 12
T17630 32129-32130 NN denotes %
T17631 32131-32134 NN denotes SDS
T17633 32134-32135 HYPH denotes -
T17632 32135-32149 NN denotes polyacrylamide
T17628 32150-32153 NN denotes gel
T17634 32154-32157 CC denotes and
T17635 32158-32169 VBN denotes transferred
T17636 32170-32172 IN denotes to
T17637 32173-32174 DT denotes a
T17639 32175-32189 NN denotes nitrocellulose
T17638 32190-32198 NN denotes membrane
T17640 32198-32199 . denotes .
T17641 32199-32445 sentence denotes Membranes were blocked in 5% nonfat milk in Tris-buffered saline with 0.05% Tween 20 (TBST), followed by an overnight incubation (at 4 °C) with AQP2 polyclonal antibody (Santa Cruz Biotechnology, Santa Cruz, California, United States; #sc-9882).
T17642 32200-32209 NNS denotes Membranes
T17644 32210-32214 VBD denotes were
T17643 32215-32222 VBN denotes blocked
T17645 32223-32225 IN denotes in
T17646 32226-32227 CD denotes 5
T17647 32227-32228 NN denotes %
T17649 32229-32235 JJ denotes nonfat
T17648 32236-32240 NN denotes milk
T17650 32241-32243 IN denotes in
T17651 32244-32248 NN denotes Tris
T17653 32248-32249 HYPH denotes -
T17652 32249-32257 VBN denotes buffered
T17654 32258-32264 NN denotes saline
T17655 32265-32269 IN denotes with
T17656 32270-32274 CD denotes 0.05
T17657 32274-32275 NN denotes %
T17658 32276-32281 NN denotes Tween
T17659 32282-32284 CD denotes 20
T17660 32285-32286 -LRB- denotes (
T17661 32286-32290 NN denotes TBST
T17662 32290-32291 -RRB- denotes )
T17663 32291-32293 , denotes ,
T17664 32293-32301 VBN denotes followed
T17665 32302-32304 IN denotes by
T17666 32305-32307 DT denotes an
T17668 32308-32317 JJ denotes overnight
T17667 32318-32328 NN denotes incubation
T17669 32329-32330 -LRB- denotes (
T17670 32330-32332 IN denotes at
T17671 32333-32334 CD denotes 4
T17672 32335-32337 NN denotes °C
T17673 32337-32338 -RRB- denotes )
T17674 32339-32343 IN denotes with
T17675 32344-32348 NN denotes AQP2
T17677 32349-32359 JJ denotes polyclonal
T17676 32360-32368 NN denotes antibody
T17678 32369-32370 -LRB- denotes (
T17680 32370-32375 NNP denotes Santa
T17681 32376-32380 NNP denotes Cruz
T17682 32381-32394 NNP denotes Biotechnology
T17683 32394-32396 , denotes ,
T17684 32396-32401 NNP denotes Santa
T17685 32402-32406 NNP denotes Cruz
T17686 32406-32408 , denotes ,
T17687 32408-32418 NNP denotes California
T17688 32418-32420 , denotes ,
T17689 32420-32426 NNP denotes United
T17690 32427-32433 NNP denotes States
T17691 32433-32434 : denotes ;
T17692 32435-32436 SYM denotes #
T17679 32436-32438 NN denotes sc
T17693 32438-32439 HYPH denotes -
T17694 32439-32443 CD denotes 9882
T17695 32443-32444 -RRB- denotes )
T17696 32444-32445 . denotes .
T17697 32445-32537 sentence denotes Membranes were washed in TBST then incubated with HRP-conjugated donkey anti-goat antibody.
T17698 32446-32455 NNS denotes Membranes
T17700 32456-32460 VBD denotes were
T17699 32461-32467 VBN denotes washed
T17701 32468-32470 IN denotes in
T17702 32471-32475 NN denotes TBST
T17703 32476-32480 RB denotes then
T17704 32481-32490 VBN denotes incubated
T17705 32491-32495 IN denotes with
T17706 32496-32499 NN denotes HRP
T17708 32499-32500 HYPH denotes -
T17707 32500-32510 VBN denotes conjugated
T17710 32511-32517 NN denotes donkey
T17711 32518-32527 JJ denotes anti-goat
T17709 32528-32536 NN denotes antibody
T17712 32536-32537 . denotes .
T17713 32537-32676 sentence denotes Membranes were washed further in TBST and bands were visualized using ECL reagent (Amersham Biosciences, Little Chalfont, United Kingdom).
T17714 32538-32547 NNS denotes Membranes
T17716 32548-32552 VBD denotes were
T17715 32553-32559 VBN denotes washed
T17717 32560-32567 RB denotes further
T17718 32568-32570 IN denotes in
T17719 32571-32575 NN denotes TBST
T17720 32576-32579 CC denotes and
T17721 32580-32585 NNS denotes bands
T17723 32586-32590 VBD denotes were
T17722 32591-32601 VBN denotes visualized
T17724 32602-32607 VBG denotes using
T17725 32608-32611 NN denotes ECL
T17726 32612-32619 NN denotes reagent
T17727 32620-32621 -LRB- denotes (
T17729 32621-32629 NNP denotes Amersham
T17728 32630-32641 NNP denotes Biosciences
T17730 32641-32643 , denotes ,
T17731 32643-32649 NNP denotes Little
T17732 32650-32658 NNP denotes Chalfont
T17733 32658-32660 , denotes ,
T17734 32660-32666 NNP denotes United
T17735 32667-32674 NNP denotes Kingdom
T17736 32674-32675 -RRB- denotes )
T17737 32675-32676 . denotes .
T17905 32678-32693 NN denotes Endoglycosidase
T17906 32694-32703 NN denotes digestion
T17907 32703-32704 . denotes .
T17908 32704-32860 sentence denotes Kidney membranes (60 μg) were incubated in 50 mM sodium phosphate (pH 5.5), 0.1% SDS, and 50 mM β-mercaptoethanol, heated to 100 °C for 5 min, then cooled.
T17909 32705-32711 NN denotes Kidney
T17910 32712-32721 NNS denotes membranes
T17912 32722-32723 -LRB- denotes (
T17914 32723-32725 CD denotes 60
T17913 32726-32728 NN denotes μg
T17915 32728-32729 -RRB- denotes )
T17916 32730-32734 VBD denotes were
T17911 32735-32744 VBN denotes incubated
T17917 32745-32747 IN denotes in
T17918 32748-32750 CD denotes 50
T17919 32751-32753 NN denotes mM
T17921 32754-32760 NN denotes sodium
T17920 32761-32770 NN denotes phosphate
T17922 32771-32772 -LRB- denotes (
T17923 32772-32774 NN denotes pH
T17924 32775-32778 CD denotes 5.5
T17925 32778-32779 -RRB- denotes )
T17926 32779-32781 , denotes ,
T17927 32781-32784 CD denotes 0.1
T17928 32784-32785 NN denotes %
T17929 32786-32789 NN denotes SDS
T17930 32789-32791 , denotes ,
T17931 32791-32794 CC denotes and
T17932 32795-32797 CD denotes 50
T17933 32798-32800 NN denotes mM
T17935 32801-32802 NN denotes β
T17936 32802-32803 HYPH denotes -
T17934 32803-32818 NN denotes mercaptoethanol
T17937 32818-32820 , denotes ,
T17938 32820-32826 VBN denotes heated
T17939 32827-32829 IN denotes to
T17940 32830-32833 CD denotes 100
T17941 32834-32836 NN denotes °C
T17942 32837-32840 IN denotes for
T17943 32841-32842 CD denotes 5
T17944 32843-32846 NN denotes min
T17945 32846-32848 , denotes ,
T17946 32848-32852 RB denotes then
T17947 32853-32859 VBN denotes cooled
T17948 32859-32860 . denotes .
T17949 32860-32948 sentence denotes Endoglycosidase H (0.01 units; Sigma-Aldrich) was added and incubated at 37 °C for 2 h.
T17950 32861-32876 NN denotes Endoglycosidase
T17951 32877-32878 NN denotes H
T17953 32879-32880 -LRB- denotes (
T17955 32880-32884 CD denotes 0.01
T17956 32885-32890 NNS denotes units
T17957 32890-32891 : denotes ;
T17958 32892-32897 NNP denotes Sigma
T17959 32897-32898 HYPH denotes -
T17954 32898-32905 NNP denotes Aldrich
T17960 32905-32906 -RRB- denotes )
T17961 32907-32910 VBD denotes was
T17952 32911-32916 VBN denotes added
T17962 32917-32920 CC denotes and
T17963 32921-32930 VBN denotes incubated
T17964 32931-32933 IN denotes at
T17965 32934-32936 CD denotes 37
T17966 32937-32939 NN denotes °C
T17967 32940-32943 IN denotes for
T17968 32944-32945 CD denotes 2
T17969 32946-32947 NN denotes h
T17970 32947-32948 . denotes .
T17971 32948-33015 sentence denotes The reaction was stopped by boiling the samples in Laemmli buffer.
T17972 32949-32952 DT denotes The
T17973 32953-32961 NN denotes reaction
T17975 32962-32965 VBD denotes was
T17974 32966-32973 VBN denotes stopped
T17976 32974-32976 IN denotes by
T17977 32977-32984 VBG denotes boiling
T17978 32985-32988 DT denotes the
T17979 32989-32996 NNS denotes samples
T17980 32997-32999 IN denotes in
T17981 33000-33007 NNP denotes Laemmli
T17982 33008-33014 NN denotes buffer
T17983 33014-33015 . denotes .
T17984 33015-33070 sentence denotes Total reactants were immunoblotted as described above.
T17985 33016-33021 JJ denotes Total
T17986 33022-33031 NNS denotes reactants
T17988 33032-33036 VBD denotes were
T17987 33037-33050 VBN denotes immunoblotted
T17989 33051-33053 IN denotes as
T17990 33054-33063 VBN denotes described
T17991 33064-33069 RB denotes above
T17992 33069-33070 . denotes .
T18783 33072-33093 NN denotes Coimmunoprecipitation
T18784 33094-33097 CC denotes and
T18785 33098-33111 NN denotes biotinylation
T18786 33112-33114 IN denotes in
T18787 33115-33119 NN denotes MDCK
T18788 33120-33125 NNS denotes cells
T18789 33125-33126 . denotes .
T18790 33126-33273 sentence denotes MDCK cells stably expressing wild-type AQP2 (grown on 10-cm plates) were transfected with pEGFP-wild-type AQP2, pEGFP-AQP2-F204V, or vector alone.
T18791 33127-33131 NN denotes MDCK
T18792 33132-33137 NNS denotes cells
T18794 33138-33144 RB denotes stably
T18795 33145-33155 VBG denotes expressing
T18796 33156-33160 JJ denotes wild
T18798 33160-33161 HYPH denotes -
T18797 33161-33165 NN denotes type
T18799 33166-33170 NN denotes AQP2
T18800 33171-33172 -LRB- denotes (
T18801 33172-33177 VBN denotes grown
T18802 33178-33180 IN denotes on
T18803 33181-33183 CD denotes 10
T18805 33183-33184 HYPH denotes -
T18804 33184-33186 NN denotes cm
T18806 33187-33193 NNS denotes plates
T18807 33193-33194 -RRB- denotes )
T18808 33195-33199 VBD denotes were
T18793 33200-33211 VBN denotes transfected
T18809 33212-33216 IN denotes with
T18810 33217-33222 NN denotes pEGFP
T18812 33222-33223 HYPH denotes -
T18813 33223-33227 JJ denotes wild
T18814 33227-33228 HYPH denotes -
T18811 33228-33232 NN denotes type
T18815 33233-33237 NN denotes AQP2
T18816 33237-33239 , denotes ,
T18817 33239-33244 NN denotes pEGFP
T18819 33244-33245 HYPH denotes -
T18820 33245-33249 NN denotes AQP2
T18821 33249-33250 HYPH denotes -
T18818 33250-33255 NN denotes F204V
T18822 33255-33257 , denotes ,
T18823 33257-33259 CC denotes or
T18824 33260-33266 NN denotes vector
T18825 33267-33272 RB denotes alone
T18826 33272-33273 . denotes .
T18827 33273-33366 sentence denotes The cells were homogenized in 10 mM Tris (pH 7.4), 1 mM EDTA, and 250 mM sucrose 40 h later.
T18828 33274-33277 DT denotes The
T18829 33278-33283 NNS denotes cells
T18831 33284-33288 VBD denotes were
T18830 33289-33300 VBN denotes homogenized
T18832 33301-33303 IN denotes in
T18833 33304-33306 CD denotes 10
T18834 33307-33309 NN denotes mM
T18835 33310-33314 NN denotes Tris
T18836 33315-33316 -LRB- denotes (
T18837 33316-33318 NN denotes pH
T18838 33319-33322 CD denotes 7.4
T18839 33322-33323 -RRB- denotes )
T18840 33323-33325 , denotes ,
T18841 33325-33326 CD denotes 1
T18842 33327-33329 NN denotes mM
T18843 33330-33334 NN denotes EDTA
T18844 33334-33336 , denotes ,
T18845 33336-33339 CC denotes and
T18846 33340-33343 CD denotes 250
T18847 33344-33346 NN denotes mM
T18848 33347-33354 NN denotes sucrose
T18849 33355-33357 CD denotes 40
T18850 33358-33359 NN denotes h
T18851 33360-33365 RB denotes later
T18852 33365-33366 . denotes .
T18853 33366-33433 sentence denotes The clarified supernatant was centrifuged at 200,000 g for 30 min.
T18854 33367-33370 DT denotes The
T18856 33371-33380 VBN denotes clarified
T18855 33381-33392 NN denotes supernatant
T18858 33393-33396 VBD denotes was
T18857 33397-33408 VBN denotes centrifuged
T18859 33409-33411 IN denotes at
T18860 33412-33419 CD denotes 200,000
T18861 33420-33421 NN denotes g
T18862 33422-33425 IN denotes for
T18863 33426-33428 CD denotes 30
T18864 33429-33432 NN denotes min
T18865 33432-33433 . denotes .
T18866 33433-33558 sentence denotes Pelleted membranes were resuspended in the same buffer but containing 4% sodium deoxycholate and incubated at 37 °C for 1 h.
T18867 33434-33442 VBN denotes Pelleted
T18868 33443-33452 NNS denotes membranes
T18870 33453-33457 VBD denotes were
T18871 33458-33469 VBN denotes resuspended
T18872 33470-33472 IN denotes in
T18873 33473-33476 DT denotes the
T18875 33477-33481 JJ denotes same
T18874 33482-33488 NN denotes buffer
T18876 33489-33492 IN denotes but
T18877 33493-33503 VBG denotes containing
T18878 33504-33505 CD denotes 4
T18879 33505-33506 NN denotes %
T18881 33507-33513 NN denotes sodium
T18880 33514-33526 NN denotes deoxycholate
T18882 33527-33530 CC denotes and
T18869 33531-33540 VBN denotes incubated
T18883 33541-33543 IN denotes at
T18884 33544-33546 CD denotes 37
T18885 33547-33549 NN denotes °C
T18886 33550-33553 IN denotes for
T18887 33554-33555 CD denotes 1
T18888 33556-33557 NN denotes h
T18889 33557-33558 . denotes .
T18890 33558-33656 sentence denotes From the dissolved membranes, a 30 μl sample was removed and used as the total membrane fraction.
T18891 33559-33563 IN denotes From
T18893 33564-33567 DT denotes the
T18895 33568-33577 VBN denotes dissolved
T18894 33578-33587 NNS denotes membranes
T18896 33587-33589 , denotes ,
T18897 33589-33590 DT denotes a
T18899 33591-33593 CD denotes 30
T18900 33594-33596 NN denotes μl
T18898 33597-33603 NN denotes sample
T18901 33604-33607 VBD denotes was
T18892 33608-33615 VBN denotes removed
T18902 33616-33619 CC denotes and
T18903 33620-33624 VBN denotes used
T18904 33625-33627 IN denotes as
T18905 33628-33631 DT denotes the
T18907 33632-33637 JJ denotes total
T18908 33638-33646 NN denotes membrane
T18906 33647-33655 NN denotes fraction
T18909 33655-33656 . denotes .
T18910 33656-33825 sentence denotes The remaining membranes were diluted with 600 μl of the homogenization buffer, and incubated with 1 μl of GFP antisera (Invitrogen, #46–0092) and protein A/G sepharose.
T18911 33657-33660 DT denotes The
T18913 33661-33670 VBG denotes remaining
T18912 33671-33680 NNS denotes membranes
T18915 33681-33685 VBD denotes were
T18914 33686-33693 VBN denotes diluted
T18916 33694-33698 IN denotes with
T18917 33699-33702 CD denotes 600
T18918 33703-33705 NN denotes μl
T18919 33706-33708 IN denotes of
T18920 33709-33712 DT denotes the
T18922 33713-33727 NN denotes homogenization
T18921 33728-33734 NN denotes buffer
T18923 33734-33736 , denotes ,
T18924 33736-33739 CC denotes and
T18925 33740-33749 VBN denotes incubated
T18926 33750-33754 IN denotes with
T18927 33755-33756 CD denotes 1
T18928 33757-33759 NN denotes μl
T18929 33760-33762 IN denotes of
T18930 33763-33766 NN denotes GFP
T18931 33767-33775 NNS denotes antisera
T18932 33776-33777 -LRB- denotes (
T18934 33777-33787 NNP denotes Invitrogen
T18935 33787-33789 , denotes ,
T18936 33789-33790 SYM denotes #
T18937 33790-33792 CD denotes 46
T18938 33792-33793 HYPH denotes –
T18933 33793-33797 CD denotes 0092
T18939 33797-33798 -RRB- denotes )
T18940 33799-33802 CC denotes and
T18941 33803-33810 NN denotes protein
T18943 33811-33812 NN denotes A
T18945 33812-33813 HYPH denotes /
T18944 33813-33814 NN denotes G
T18942 33815-33824 NN denotes sepharose
T18946 33824-33825 . denotes .
T18947 33825-33957 sentence denotes Following overnight incubation, the precipitated proteins were washed in RIPA buffer and finally boiled in 50 μl of Laemmli buffer.
T18948 33826-33835 VBG denotes Following
T18950 33836-33845 JJ denotes overnight
T18951 33846-33856 NN denotes incubation
T18952 33856-33858 , denotes ,
T18953 33858-33861 DT denotes the
T18955 33862-33874 VBN denotes precipitated
T18954 33875-33883 NN denotes proteins
T18956 33884-33888 VBD denotes were
T18949 33889-33895 VBN denotes washed
T18957 33896-33898 IN denotes in
T18958 33899-33903 NN denotes RIPA
T18959 33904-33910 NN denotes buffer
T18960 33911-33914 CC denotes and
T18961 33915-33922 RB denotes finally
T18962 33923-33929 VBN denotes boiled
T18963 33930-33932 IN denotes in
T18964 33933-33935 CD denotes 50
T18965 33936-33938 NN denotes μl
T18966 33939-33941 IN denotes of
T18967 33942-33949 NNP denotes Laemmli
T18968 33950-33956 NN denotes buffer
T18969 33956-33957 . denotes .
T18970 33957-34040 sentence denotes Half of the total membrane and the IP fractions were processed for immunoblotting.
T18971 33958-33962 NN denotes Half
T18973 33963-33965 IN denotes of
T18974 33966-33969 DT denotes the
T18976 33970-33975 JJ denotes total
T18975 33976-33984 NN denotes membrane
T18977 33985-33988 CC denotes and
T18978 33989-33992 DT denotes the
T18980 33993-33995 NN denotes IP
T18979 33996-34005 NNS denotes fractions
T18981 34006-34010 VBD denotes were
T18972 34011-34020 VBN denotes processed
T18982 34021-34024 IN denotes for
T18983 34025-34039 NN denotes immunoblotting
T18984 34039-34040 . denotes .
T18985 34040-34102 sentence denotes Cell surface biotinylation was performed in a similar manner.
T18986 34041-34045 NN denotes Cell
T18988 34046-34053 NN denotes surface
T18987 34054-34067 NN denotes biotinylation
T18990 34068-34071 VBD denotes was
T18989 34072-34081 VBN denotes performed
T18991 34082-34084 IN denotes in
T18992 34085-34086 DT denotes a
T18994 34087-34094 JJ denotes similar
T18993 34095-34101 NN denotes manner
T18995 34101-34102 . denotes .
T18996 34102-34235 sentence denotes However, pEGFP-AQP2-F204V, was transfected into MDCK cells stably expressing wild-type AQP2 and cells made stable with vector alone.
T18997 34103-34110 RB denotes However
T18999 34110-34112 , denotes ,
T19000 34112-34117 NN denotes pEGFP
T19002 34117-34118 HYPH denotes -
T19003 34118-34122 NN denotes AQP2
T19004 34122-34123 HYPH denotes -
T19001 34123-34128 NN denotes F204V
T19005 34128-34130 , denotes ,
T19006 34130-34133 VBD denotes was
T18998 34134-34145 VBN denotes transfected
T19007 34146-34150 IN denotes into
T19008 34151-34155 NN denotes MDCK
T19009 34156-34161 NNS denotes cells
T19010 34162-34168 RB denotes stably
T19011 34169-34179 VBG denotes expressing
T19012 34180-34184 JJ denotes wild
T19014 34184-34185 HYPH denotes -
T19013 34185-34189 NN denotes type
T19015 34190-34194 NN denotes AQP2
T19016 34195-34198 CC denotes and
T19017 34199-34204 NNS denotes cells
T19018 34205-34209 VBN denotes made
T19019 34210-34216 JJ denotes stable
T19020 34217-34221 IN denotes with
T19021 34222-34228 NN denotes vector
T19022 34229-34234 RB denotes alone
T19023 34234-34235 . denotes .
T19024 34235-34472 sentence denotes Twenty-four hours post-transfection, cells were stimulated with forskolin, trypsinized, resuspended in 1 ml of PBS (2.5 × 106 cells/ml), and incubated with 0.5 mg of NHS-PEO4-biotin (Pierce Biotechnology) for 30 min at room temperature.
T19025 34236-34242 CD denotes Twenty
T19027 34242-34243 HYPH denotes -
T19026 34243-34247 CD denotes four
T19028 34248-34253 NNS denotes hours
T19029 34254-34271 RB denotes post-transfection
T19031 34271-34273 , denotes ,
T19032 34273-34278 NNS denotes cells
T19033 34279-34283 VBD denotes were
T19030 34284-34294 VBN denotes stimulated
T19034 34295-34299 IN denotes with
T19035 34300-34309 NN denotes forskolin
T19036 34309-34311 , denotes ,
T19037 34311-34322 VBN denotes trypsinized
T19038 34322-34324 , denotes ,
T19039 34324-34335 VBN denotes resuspended
T19040 34336-34338 IN denotes in
T19041 34339-34340 CD denotes 1
T19042 34341-34343 NN denotes ml
T19043 34344-34346 IN denotes of
T19044 34347-34350 NN denotes PBS
T19045 34351-34352 -LRB- denotes (
T19047 34352-34355 CD denotes 2.5
T19049 34356-34357 SYM denotes ×
T19048 34358-34361 CD denotes 106
T19046 34362-34367 NNS denotes cells
T19050 34367-34368 SYM denotes /
T19051 34368-34370 NNS denotes ml
T19052 34370-34371 -RRB- denotes )
T19053 34371-34373 , denotes ,
T19054 34373-34376 CC denotes and
T19055 34377-34386 VBN denotes incubated
T19056 34387-34391 IN denotes with
T19057 34392-34395 CD denotes 0.5
T19058 34396-34398 NN denotes mg
T19059 34399-34401 IN denotes of
T19060 34402-34405 NN denotes NHS
T19062 34405-34406 HYPH denotes -
T19063 34406-34410 NN denotes PEO4
T19064 34410-34411 HYPH denotes -
T19061 34411-34417 NN denotes biotin
T19065 34418-34419 -LRB- denotes (
T19067 34419-34425 NNP denotes Pierce
T19066 34426-34439 NNP denotes Biotechnology
T19068 34439-34440 -RRB- denotes )
T19069 34441-34444 IN denotes for
T19070 34445-34447 CD denotes 30
T19071 34448-34451 NN denotes min
T19072 34452-34454 IN denotes at
T19073 34455-34459 NN denotes room
T19074 34460-34471 NN denotes temperature
T19075 34471-34472 . denotes .
T19076 34472-34612 sentence denotes Cells were washed once in 10 mM Tris (pH 8) and three times in PBS, after which membranes were purified and solubilized as described above.
T19077 34473-34478 NNS denotes Cells
T19079 34479-34483 VBD denotes were
T19078 34484-34490 VBN denotes washed
T19080 34491-34495 RB denotes once
T19081 34496-34498 IN denotes in
T19082 34499-34501 CD denotes 10
T19083 34502-34504 NN denotes mM
T19084 34505-34509 NN denotes Tris
T19085 34510-34511 -LRB- denotes (
T19086 34511-34513 NN denotes pH
T19087 34514-34515 CD denotes 8
T19088 34515-34516 -RRB- denotes )
T19089 34517-34520 CC denotes and
T19090 34521-34526 CD denotes three
T19091 34527-34532 NNS denotes times
T19092 34533-34535 IN denotes in
T19093 34536-34539 NN denotes PBS
T19094 34539-34541 , denotes ,
T19095 34541-34546 IN denotes after
T19097 34547-34552 WDT denotes which
T19098 34553-34562 NNS denotes membranes
T19099 34563-34567 VBD denotes were
T19096 34568-34576 VBN denotes purified
T19100 34577-34580 CC denotes and
T19101 34581-34592 VBN denotes solubilized
T19102 34593-34595 IN denotes as
T19103 34596-34605 VBN denotes described
T19104 34606-34611 RB denotes above
T19105 34611-34612 . denotes .
T19106 34612-34728 sentence denotes Solubilized membranes were incubated with 20 μl of immobilized streptavidin (Pierce Biotechnology) for 2 h at 4 °C.
T19107 34613-34624 VBN denotes Solubilized
T19108 34625-34634 NNS denotes membranes
T19110 34635-34639 VBD denotes were
T19109 34640-34649 VBN denotes incubated
T19111 34650-34654 IN denotes with
T19112 34655-34657 CD denotes 20
T19113 34658-34660 NN denotes μl
T19114 34661-34663 IN denotes of
T19115 34664-34675 VBN denotes immobilized
T19116 34676-34688 NN denotes streptavidin
T19117 34689-34690 -LRB- denotes (
T19119 34690-34696 NNP denotes Pierce
T19118 34697-34710 NNP denotes Biotechnology
T19120 34710-34711 -RRB- denotes )
T19121 34712-34715 IN denotes for
T19122 34716-34717 CD denotes 2
T19123 34718-34719 NN denotes h
T19124 34720-34722 IN denotes at
T19125 34723-34724 CD denotes 4
T19126 34725-34727 NN denotes °C
T19127 34727-34728 . denotes .
T19128 34728-34828 sentence denotes Finally the precipitated proteins were washed in RIPA buffer and boiled in 50 μl of Laemmli buffer.
T19129 34729-34736 RB denotes Finally
T19131 34737-34740 DT denotes the
T19133 34741-34753 VBN denotes precipitated
T19132 34754-34762 NN denotes proteins
T19134 34763-34767 VBD denotes were
T19130 34768-34774 VBN denotes washed
T19135 34775-34777 IN denotes in
T19136 34778-34782 NN denotes RIPA
T19137 34783-34789 NN denotes buffer
T19138 34790-34793 CC denotes and
T19139 34794-34800 VBN denotes boiled
T19140 34801-34803 IN denotes in
T19141 34804-34806 CD denotes 50
T19142 34807-34809 NN denotes μl
T19143 34810-34812 IN denotes of
T19144 34813-34820 NNP denotes Laemmli
T19145 34821-34827 NN denotes buffer
T19146 34827-34828 . denotes .
T19147 34828-34921 sentence denotes Total cells and the biotinylated precipitates were immunoblotting using an antibody to AQP2.
T19148 34829-34834 JJ denotes Total
T19149 34835-34840 NNS denotes cells
T19151 34841-34844 CC denotes and
T19152 34845-34848 DT denotes the
T19154 34849-34861 VBN denotes biotinylated
T19153 34862-34874 NNS denotes precipitates
T19155 34875-34879 VBD denotes were
T19150 34880-34894 VBG denotes immunoblotting
T19156 34895-34900 VBG denotes using
T19157 34901-34903 DT denotes an
T19158 34904-34912 NN denotes antibody
T19159 34913-34915 IN denotes to
T19160 34916-34920 NN denotes AQP2
T19161 34920-34921 . denotes .
T19603 34923-34929 NN denotes Kidney
T19604 34930-34950 NN denotes immunohistochemistry
T19605 34950-34951 . denotes .
T19606 34951-35027 sentence denotes Whole mouse kidneys were fixed in 10% phosphate-buffered formalin for 24 h.
T19607 34952-34957 JJ denotes Whole
T19609 34958-34963 NN denotes mouse
T19608 34964-34971 NNS denotes kidneys
T19611 34972-34976 VBD denotes were
T19610 34977-34982 VBN denotes fixed
T19612 34983-34985 IN denotes in
T19613 34986-34988 CD denotes 10
T19614 34988-34989 NN denotes %
T19616 34990-34999 NN denotes phosphate
T19618 34999-35000 HYPH denotes -
T19617 35000-35008 VBN denotes buffered
T19615 35009-35017 NN denotes formalin
T19619 35018-35021 IN denotes for
T19620 35022-35024 CD denotes 24
T19621 35025-35026 NN denotes h
T19622 35026-35027 . denotes .
T19623 35027-35095 sentence denotes Kidneys were embedded in paraffin, and 5-μm sections were prepared.
T19624 35028-35035 NNS denotes Kidneys
T19626 35036-35040 VBD denotes were
T19625 35041-35049 VBN denotes embedded
T19627 35050-35052 IN denotes in
T19628 35053-35061 NN denotes paraffin
T19629 35061-35063 , denotes ,
T19630 35063-35066 CC denotes and
T19631 35067-35068 CD denotes 5
T19633 35068-35069 HYPH denotes -
T19632 35069-35071 NN denotes μm
T19634 35072-35080 NNS denotes sections
T19636 35081-35085 VBD denotes were
T19635 35086-35094 VBN denotes prepared
T19637 35094-35095 . denotes .
T19638 35095-35247 sentence denotes Following antigen retrieval using 10 mM sodium citrate (pH 8) for 10 min at 98 °C, sections were sequentially probed, first for AQP3 and then for AQP2.
T19639 35096-35105 VBG denotes Following
T19641 35106-35113 NN denotes antigen
T19642 35114-35123 NN denotes retrieval
T19643 35124-35129 VBG denotes using
T19644 35130-35132 CD denotes 10
T19645 35133-35135 NN denotes mM
T19647 35136-35142 NN denotes sodium
T19646 35143-35150 NN denotes citrate
T19648 35151-35152 -LRB- denotes (
T19649 35152-35154 NN denotes pH
T19650 35155-35156 CD denotes 8
T19651 35156-35157 -RRB- denotes )
T19652 35158-35161 IN denotes for
T19653 35162-35164 CD denotes 10
T19654 35165-35168 NN denotes min
T19655 35169-35171 IN denotes at
T19656 35172-35174 CD denotes 98
T19657 35175-35177 NN denotes °C
T19658 35177-35179 , denotes ,
T19659 35179-35187 NNS denotes sections
T19660 35188-35192 VBD denotes were
T19661 35193-35205 RB denotes sequentially
T19640 35206-35212 VBN denotes probed
T19662 35212-35214 , denotes ,
T19663 35214-35219 RB denotes first
T19664 35220-35223 IN denotes for
T19665 35224-35228 NN denotes AQP3
T19666 35229-35232 CC denotes and
T19667 35233-35237 RB denotes then
T19668 35238-35241 IN denotes for
T19669 35242-35246 NN denotes AQP2
T19670 35246-35247 . denotes .
T19671 35247-35371 sentence denotes Sections were incubated in 5% donkey serum and then in goat anti-AQP3 antibody (1:100; Santa Cruz Biotechnology; #sc-9885).
T19672 35248-35256 NNS denotes Sections
T19674 35257-35261 VBD denotes were
T19673 35262-35271 VBN denotes incubated
T19675 35272-35274 IN denotes in
T19676 35275-35276 CD denotes 5
T19677 35276-35277 NN denotes %
T19679 35278-35284 NN denotes donkey
T19678 35285-35290 NN denotes serum
T19680 35291-35294 CC denotes and
T19681 35295-35299 RB denotes then
T19682 35300-35302 IN denotes in
T19683 35303-35307 NN denotes goat
T19685 35308-35317 JJ denotes anti-AQP3
T19684 35318-35326 NN denotes antibody
T19686 35327-35328 -LRB- denotes (
T19688 35328-35329 CD denotes 1
T19689 35329-35330 SYM denotes :
T19690 35330-35333 CD denotes 100
T19691 35333-35334 : denotes ;
T19692 35335-35340 NNP denotes Santa
T19693 35341-35345 NNP denotes Cruz
T19694 35346-35359 NNP denotes Biotechnology
T19695 35359-35360 : denotes ;
T19696 35361-35362 SYM denotes #
T19687 35362-35364 NN denotes sc
T19697 35364-35365 HYPH denotes -
T19698 35365-35369 CD denotes 9885
T19699 35369-35370 -RRB- denotes )
T19700 35370-35371 . denotes .
T19701 35371-35521 sentence denotes Slides were washed with PBS and incubated with AlexaFluor 488-conjugated donkey anti-goat antibody (Molecular Probes, Eugene, Oregon, United States).
T19702 35372-35378 NNS denotes Slides
T19704 35379-35383 VBD denotes were
T19703 35384-35390 VBN denotes washed
T19705 35391-35395 IN denotes with
T19706 35396-35399 NN denotes PBS
T19707 35400-35403 CC denotes and
T19708 35404-35413 VBN denotes incubated
T19709 35414-35418 IN denotes with
T19710 35419-35429 NNP denotes AlexaFluor
T19712 35430-35433 CD denotes 488
T19713 35433-35434 HYPH denotes -
T19711 35434-35444 VBN denotes conjugated
T19715 35445-35451 NN denotes donkey
T19716 35452-35461 JJ denotes anti-goat
T19714 35462-35470 NN denotes antibody
T19717 35471-35472 -LRB- denotes (
T19719 35472-35481 NNP denotes Molecular
T19718 35482-35488 NNP denotes Probes
T19720 35488-35490 , denotes ,
T19721 35490-35496 NNP denotes Eugene
T19722 35496-35498 , denotes ,
T19723 35498-35504 NNP denotes Oregon
T19724 35504-35506 , denotes ,
T19725 35506-35512 NNP denotes United
T19726 35513-35519 NNP denotes States
T19727 35519-35520 -RRB- denotes )
T19728 35520-35521 . denotes .
T19729 35521-35787 sentence denotes The slides were subsequently blocked in 5% chicken serum, incubated with a rabbit anti-AQP2 antibody (1:250; USB, Cleveland, Ohio, United States; #A3000–06), which was detected with a AlexaFluor 594-conjugated chicken anti-rabbit antibody (1:500; Molecular Probes).
T19730 35522-35525 DT denotes The
T19731 35526-35532 NNS denotes slides
T19733 35533-35537 VBD denotes were
T19734 35538-35550 RB denotes subsequently
T19735 35551-35558 VBN denotes blocked
T19736 35559-35561 IN denotes in
T19737 35562-35563 CD denotes 5
T19738 35563-35564 NN denotes %
T19740 35565-35572 NN denotes chicken
T19739 35573-35578 NN denotes serum
T19741 35578-35580 , denotes ,
T19732 35580-35589 VBN denotes incubated
T19742 35590-35594 IN denotes with
T19743 35595-35596 DT denotes a
T19745 35597-35603 NN denotes rabbit
T19746 35604-35613 JJ denotes anti-AQP2
T19744 35614-35622 NN denotes antibody
T19747 35623-35624 -LRB- denotes (
T19749 35624-35625 CD denotes 1
T19750 35625-35626 SYM denotes :
T19751 35626-35629 CD denotes 250
T19752 35629-35630 : denotes ;
T19753 35631-35634 NNP denotes USB
T19754 35634-35636 , denotes ,
T19755 35636-35645 NNP denotes Cleveland
T19756 35645-35647 , denotes ,
T19757 35647-35651 NNP denotes Ohio
T19758 35651-35653 , denotes ,
T19759 35653-35659 NNP denotes United
T19760 35660-35666 NNP denotes States
T19761 35666-35667 : denotes ;
T19762 35668-35669 SYM denotes #
T19748 35669-35674 NN denotes A3000
T19763 35674-35675 HYPH denotes –
T19764 35675-35677 CD denotes 06
T19765 35677-35678 -RRB- denotes )
T19766 35678-35680 , denotes ,
T19767 35680-35685 WDT denotes which
T19769 35686-35689 VBD denotes was
T19768 35690-35698 VBN denotes detected
T19770 35699-35703 IN denotes with
T19771 35704-35705 DT denotes a
T19773 35706-35716 NNP denotes AlexaFluor
T19775 35717-35720 CD denotes 594
T19776 35720-35721 HYPH denotes -
T19774 35721-35731 VBN denotes conjugated
T19777 35732-35739 NN denotes chicken
T19778 35740-35751 JJ denotes anti-rabbit
T19772 35752-35760 NN denotes antibody
T19779 35761-35762 -LRB- denotes (
T19781 35762-35763 CD denotes 1
T19782 35763-35764 SYM denotes :
T19783 35764-35767 CD denotes 500
T19784 35767-35768 : denotes ;
T19785 35769-35778 NNP denotes Molecular
T19780 35779-35785 NNP denotes Probes
T19786 35785-35786 -RRB- denotes )
T19787 35786-35787 . denotes .
T19788 35787-35904 sentence denotes The sections were stained with DAPI and mounted in Vectashield (Vector Labs, Burlingame, California, United States).
T19789 35788-35791 DT denotes The
T19790 35792-35800 NNS denotes sections
T19792 35801-35805 VBD denotes were
T19791 35806-35813 VBN denotes stained
T19793 35814-35818 IN denotes with
T19794 35819-35823 NN denotes DAPI
T19795 35824-35827 CC denotes and
T19796 35828-35835 VBD denotes mounted
T19797 35836-35838 IN denotes in
T19798 35839-35850 NNP denotes Vectashield
T19799 35851-35852 -LRB- denotes (
T19801 35852-35858 NNP denotes Vector
T19800 35859-35863 NNP denotes Labs
T19802 35863-35865 , denotes ,
T19803 35865-35875 NNP denotes Burlingame
T19804 35875-35877 , denotes ,
T19805 35877-35887 NNP denotes California
T19806 35887-35889 , denotes ,
T19807 35889-35895 NNP denotes United
T19808 35896-35902 NNP denotes States
T19809 35902-35903 -RRB- denotes )
T19810 35903-35904 . denotes .
T20424 35906-35925 NN denotes Immunocytochemistry
T20425 35926-35928 IN denotes on
T20426 35929-35933 NN denotes MDCK
T20427 35934-35939 NNS denotes cells
T20428 35939-35940 . denotes .
T20429 35940-36151 sentence denotes MDCK stable cell lines expressing vector alone, wild-type AQP2, or AQP2-F204V (and in some cases transiently expressing a GFP construct) were grown on fibronectin-coated coverslips until tight junctions formed.
T20430 35941-35945 NN denotes MDCK
T20432 35946-35952 JJ denotes stable
T20433 35953-35957 NN denotes cell
T20431 35958-35963 NNS denotes lines
T20435 35964-35974 VBG denotes expressing
T20436 35975-35981 NN denotes vector
T20437 35982-35987 JJ denotes alone
T20438 35987-35989 , denotes ,
T20439 35989-35993 JJ denotes wild
T20441 35993-35994 HYPH denotes -
T20440 35994-35998 NN denotes type
T20442 35999-36003 NN denotes AQP2
T20443 36003-36005 , denotes ,
T20444 36005-36007 CC denotes or
T20445 36008-36012 NN denotes AQP2
T20447 36012-36013 HYPH denotes -
T20446 36013-36018 NN denotes F204V
T20448 36019-36020 -LRB- denotes (
T20449 36020-36023 CC denotes and
T20450 36024-36026 IN denotes in
T20452 36027-36031 DT denotes some
T20453 36032-36037 NNS denotes cases
T20454 36038-36049 RB denotes transiently
T20451 36050-36060 VBG denotes expressing
T20455 36061-36062 DT denotes a
T20457 36063-36066 NN denotes GFP
T20456 36067-36076 NN denotes construct
T20458 36076-36077 -RRB- denotes )
T20459 36078-36082 VBD denotes were
T20434 36083-36088 VBN denotes grown
T20460 36089-36091 IN denotes on
T20461 36092-36103 NN denotes fibronectin
T20463 36103-36104 HYPH denotes -
T20462 36104-36110 VBN denotes coated
T20464 36111-36121 NNS denotes coverslips
T20465 36122-36127 IN denotes until
T20467 36128-36133 JJ denotes tight
T20468 36134-36143 NNS denotes junctions
T20466 36144-36150 VBD denotes formed
T20469 36150-36151 . denotes .
T20470 36151-36248 sentence denotes Cells were treated with or without 150 μM forskolin for 90 min, and fixed in methanol at −20 °C.
T20471 36152-36157 NNS denotes Cells
T20473 36158-36162 VBD denotes were
T20472 36163-36170 VBN denotes treated
T20474 36171-36175 IN denotes with
T20475 36176-36178 CC denotes or
T20476 36179-36186 IN denotes without
T20477 36187-36190 CD denotes 150
T20478 36191-36193 NN denotes μM
T20479 36194-36203 NN denotes forskolin
T20480 36204-36207 IN denotes for
T20481 36208-36210 CD denotes 90
T20482 36211-36214 NN denotes min
T20483 36214-36216 , denotes ,
T20484 36216-36219 CC denotes and
T20485 36220-36225 VBN denotes fixed
T20486 36226-36228 IN denotes in
T20487 36229-36237 NN denotes methanol
T20488 36238-36240 IN denotes at
T20489 36241-36242 SYM denotes −
T20490 36242-36244 CD denotes 20
T20491 36245-36247 NN denotes °C
T20492 36247-36248 . denotes .
T20493 36248-36414 sentence denotes Subsequently, cells were washed and permeabilized in 0.2% Triton X-100 for 5 min, and sequentially probed for AQP2 and organelle markers for either the PM or the ER.
T20494 36249-36261 RB denotes Subsequently
T20496 36261-36263 , denotes ,
T20497 36263-36268 NNS denotes cells
T20498 36269-36273 VBD denotes were
T20495 36274-36280 VBN denotes washed
T20499 36281-36284 CC denotes and
T20500 36285-36298 VBN denotes permeabilized
T20501 36299-36301 IN denotes in
T20502 36302-36305 CD denotes 0.2
T20503 36305-36306 NN denotes %
T20505 36307-36313 NN denotes Triton
T20504 36314-36315 NN denotes X
T20506 36315-36316 HYPH denotes -
T20507 36316-36319 CD denotes 100
T20508 36320-36323 IN denotes for
T20509 36324-36325 CD denotes 5
T20510 36326-36329 NN denotes min
T20511 36329-36331 , denotes ,
T20512 36331-36334 CC denotes and
T20513 36335-36347 RB denotes sequentially
T20514 36348-36354 VBN denotes probed
T20515 36355-36358 IN denotes for
T20516 36359-36363 NN denotes AQP2
T20517 36364-36367 CC denotes and
T20518 36368-36377 NN denotes organelle
T20519 36378-36385 NNS denotes markers
T20520 36386-36389 IN denotes for
T20521 36390-36396 CC denotes either
T20523 36397-36400 DT denotes the
T20522 36401-36403 NN denotes PM
T20524 36404-36406 CC denotes or
T20525 36407-36410 DT denotes the
T20526 36411-36413 NN denotes ER
T20527 36413-36414 . denotes .
T20528 36414-36584 sentence denotes AQP2 was detected using goat anti-AQP2 (1:100; Santa Cruz Biotechnology; #sc-9882) and a 1:200 dilution of AlexaFluor 488-conjugated donkey anti-goat secondary antibody.
T20529 36415-36419 NN denotes AQP2
T20531 36420-36423 VBD denotes was
T20530 36424-36432 VBN denotes detected
T20532 36433-36438 VBG denotes using
T20533 36439-36443 NN denotes goat
T20534 36444-36453 JJ denotes anti-AQP2
T20535 36454-36455 -LRB- denotes (
T20537 36455-36456 CD denotes 1
T20538 36456-36457 SYM denotes :
T20539 36457-36460 CD denotes 100
T20540 36460-36461 : denotes ;
T20541 36462-36467 NNP denotes Santa
T20542 36468-36472 NNP denotes Cruz
T20543 36473-36486 NNP denotes Biotechnology
T20544 36486-36487 : denotes ;
T20545 36488-36489 SYM denotes #
T20536 36489-36491 NN denotes sc
T20546 36491-36492 HYPH denotes -
T20547 36492-36496 CD denotes 9882
T20548 36496-36497 -RRB- denotes )
T20549 36498-36501 CC denotes and
T20550 36502-36503 DT denotes a
T20552 36504-36505 CD denotes 1
T20554 36505-36506 SYM denotes :
T20553 36506-36509 CD denotes 200
T20551 36510-36518 NN denotes dilution
T20555 36519-36521 IN denotes of
T20556 36522-36532 NNP denotes AlexaFluor
T20558 36533-36536 CD denotes 488
T20559 36536-36537 HYPH denotes -
T20557 36537-36547 VBN denotes conjugated
T20561 36548-36554 NN denotes donkey
T20562 36555-36564 JJ denotes anti-goat
T20563 36565-36574 JJ denotes secondary
T20560 36575-36583 NN denotes antibody
T20564 36583-36584 . denotes .
T20565 36584-36992 sentence denotes The PM and ER were probed using mouse anti-Na+/K+-ATPase (Upstate, Waltham, Massachusetts, United States) or rabbit anti-calnexin (Stressgen Biotechnology, Victoria, British Columbia, Canada) antibodies and the secondary antibodies, Cy3-conjugated goat anti-mouse (1:200; Jackson ImmunoResearch, West Grove, Pennsylvania, United States) or AlexaFluor 594-conjugated chicken anti-rabbit (1:200) respectively.
T20566 36585-36588 DT denotes The
T20567 36589-36591 NN denotes PM
T20569 36592-36595 CC denotes and
T20570 36596-36598 NN denotes ER
T20571 36599-36603 VBD denotes were
T20568 36604-36610 VBN denotes probed
T20572 36611-36616 VBG denotes using
T20573 36617-36622 NN denotes mouse
T20575 36623-36631 JJ denotes anti-Na+
T20576 36631-36632 HYPH denotes /
T20577 36632-36634 NN denotes K+
T20578 36634-36635 HYPH denotes -
T20574 36635-36641 NN denotes ATPase
T20580 36642-36643 -LRB- denotes (
T20581 36643-36650 NNP denotes Upstate
T20582 36650-36652 , denotes ,
T20583 36652-36659 NNP denotes Waltham
T20584 36659-36661 , denotes ,
T20585 36661-36674 NNP denotes Massachusetts
T20586 36674-36676 , denotes ,
T20587 36676-36682 NNP denotes United
T20588 36683-36689 NNP denotes States
T20589 36689-36690 -RRB- denotes )
T20590 36691-36693 CC denotes or
T20591 36694-36700 NN denotes rabbit
T20592 36701-36714 JJ denotes anti-calnexin
T20593 36715-36716 -LRB- denotes (
T20595 36716-36725 NNP denotes Stressgen
T20594 36726-36739 NNP denotes Biotechnology
T20596 36739-36741 , denotes ,
T20597 36741-36749 NNP denotes Victoria
T20598 36749-36751 , denotes ,
T20599 36751-36758 NNP denotes British
T20600 36759-36767 NNP denotes Columbia
T20601 36767-36769 , denotes ,
T20602 36769-36775 NNP denotes Canada
T20603 36775-36776 -RRB- denotes )
T20579 36777-36787 NNS denotes antibodies
T20604 36788-36791 CC denotes and
T20605 36792-36795 DT denotes the
T20607 36796-36805 JJ denotes secondary
T20606 36806-36816 NNS denotes antibodies
T20608 36816-36818 , denotes ,
T20609 36818-36821 NN denotes Cy3
T20611 36821-36822 HYPH denotes -
T20610 36822-36832 VBN denotes conjugated
T20612 36833-36837 NN denotes goat
T20613 36838-36848 JJ denotes anti-mouse
T20614 36849-36850 -LRB- denotes (
T20616 36850-36851 CD denotes 1
T20617 36851-36852 SYM denotes :
T20618 36852-36855 CD denotes 200
T20619 36855-36856 : denotes ;
T20620 36857-36864 NNP denotes Jackson
T20615 36865-36879 NNP denotes ImmunoResearch
T20621 36879-36881 , denotes ,
T20622 36881-36885 NNP denotes West
T20623 36886-36891 NNP denotes Grove
T20624 36891-36893 , denotes ,
T20625 36893-36905 NNP denotes Pennsylvania
T20626 36905-36907 , denotes ,
T20627 36907-36913 NNP denotes United
T20628 36914-36920 NNP denotes States
T20629 36920-36921 -RRB- denotes )
T20630 36922-36924 CC denotes or
T20631 36925-36935 NNP denotes AlexaFluor
T20633 36936-36939 CD denotes 594
T20634 36939-36940 HYPH denotes -
T20632 36940-36950 VBN denotes conjugated
T20635 36951-36958 NN denotes chicken
T20636 36959-36970 JJ denotes anti-rabbit
T20637 36971-36972 -LRB- denotes (
T20638 36972-36973 CD denotes 1
T20639 36973-36974 SYM denotes :
T20640 36974-36977 CD denotes 200
T20641 36977-36978 -RRB- denotes )
T20642 36979-36991 RB denotes respectively
T20643 36991-36992 . denotes .
T20644 36992-37072 sentence denotes Cells were washed in PBS, counterstained with DAPI, and mounted in Vectashield.
T20645 36993-36998 NNS denotes Cells
T20647 36999-37003 VBD denotes were
T20646 37004-37010 VBN denotes washed
T20648 37011-37013 IN denotes in
T20649 37014-37017 NN denotes PBS
T20650 37017-37019 , denotes ,
T20651 37019-37033 VBN denotes counterstained
T20652 37034-37038 IN denotes with
T20653 37039-37043 NN denotes DAPI
T20654 37043-37045 , denotes ,
T20655 37045-37048 CC denotes and
T20656 37049-37056 VBN denotes mounted
T20657 37057-37059 IN denotes in
T20658 37060-37071 NNP denotes Vectashield
T20659 37071-37072 . denotes .
T20660 37072-37290 sentence denotes In experiments in which GFP fusions were used, AQP2 was probed using the antibody combination used for kidney immunohistochemistry in order to detect the AQP2 at 594 nm, to distinguish between the GFP fusion proteins.
T20661 37073-37075 IN denotes In
T20663 37076-37087 NNS denotes experiments
T20664 37088-37090 IN denotes in
T20666 37091-37096 WDT denotes which
T20667 37097-37100 NN denotes GFP
T20668 37101-37108 NNS denotes fusions
T20669 37109-37113 VBD denotes were
T20665 37114-37118 VBN denotes used
T20670 37118-37120 , denotes ,
T20671 37120-37124 NN denotes AQP2
T20672 37125-37128 VBD denotes was
T20662 37129-37135 VBN denotes probed
T20673 37136-37141 VBG denotes using
T20674 37142-37145 DT denotes the
T20676 37146-37154 NN denotes antibody
T20675 37155-37166 NN denotes combination
T20677 37167-37171 VBN denotes used
T20678 37172-37175 IN denotes for
T20679 37176-37182 NN denotes kidney
T20680 37183-37203 NN denotes immunohistochemistry
T20681 37204-37206 IN denotes in
T20682 37207-37212 NN denotes order
T20683 37213-37215 TO denotes to
T20684 37216-37222 VB denotes detect
T20685 37223-37226 DT denotes the
T20686 37227-37231 NN denotes AQP2
T20687 37232-37234 IN denotes at
T20688 37235-37238 CD denotes 594
T20689 37239-37241 NN denotes nm
T20690 37241-37243 , denotes ,
T20691 37243-37245 TO denotes to
T20692 37246-37257 VB denotes distinguish
T20693 37258-37265 IN denotes between
T20694 37266-37269 DT denotes the
T20696 37270-37273 NN denotes GFP
T20697 37274-37280 NN denotes fusion
T20695 37281-37289 NN denotes proteins
T20698 37289-37290 . denotes .
T20832 37292-37300 JJ denotes Confocal
T20833 37301-37311 NN denotes microscopy
T20834 37311-37312 . denotes .
T20835 37312-37460 sentence denotes Optical z-section images were collected on a BioRad (Hercules, California, United States) Rainbow Radiance 2100 Laser Scanning Confocal Microscope.
T20836 37313-37320 JJ denotes Optical
T20838 37321-37322 NN denotes z
T20840 37322-37323 HYPH denotes -
T20839 37323-37330 NN denotes section
T20837 37331-37337 NNS denotes images
T20842 37338-37342 VBD denotes were
T20841 37343-37352 VBN denotes collected
T20843 37353-37355 IN denotes on
T20844 37356-37357 DT denotes a
T20846 37358-37364 NNP denotes BioRad
T20847 37365-37366 -LRB- denotes (
T20848 37366-37374 NNP denotes Hercules
T20849 37374-37376 , denotes ,
T20850 37376-37386 NNP denotes California
T20851 37386-37388 , denotes ,
T20852 37388-37394 NNP denotes United
T20853 37395-37401 NNP denotes States
T20854 37401-37402 -RRB- denotes )
T20855 37403-37410 NNP denotes Rainbow
T20856 37411-37419 NNP denotes Radiance
T20857 37420-37424 CD denotes 2100
T20858 37425-37430 NN denotes Laser
T20859 37431-37439 NN denotes Scanning
T20860 37440-37448 NN denotes Confocal
T20845 37449-37459 NN denotes Microscope
T20861 37459-37460 . denotes .
T20862 37460-37645 sentence denotes Image stacks were flattened, or sectioned along the z-axis, then further processed using BioRad Laser Sharp 2000 software and Image J software (v. 1.32; National Institutes of Health).
T20863 37461-37466 NN denotes Image
T20864 37467-37473 NNS denotes stacks
T20866 37474-37478 VBD denotes were
T20865 37479-37488 VBN denotes flattened
T20867 37488-37490 , denotes ,
T20868 37490-37492 CC denotes or
T20869 37493-37502 VBN denotes sectioned
T20870 37503-37508 IN denotes along
T20871 37509-37512 DT denotes the
T20873 37513-37514 NN denotes z
T20874 37514-37515 HYPH denotes -
T20872 37515-37519 NN denotes axis
T20875 37519-37521 , denotes ,
T20876 37521-37525 RB denotes then
T20878 37526-37533 RB denotes further
T20877 37534-37543 VBN denotes processed
T20879 37544-37549 VBG denotes using
T20880 37550-37556 NNP denotes BioRad
T20882 37557-37562 NNP denotes Laser
T20883 37563-37568 NNP denotes Sharp
T20884 37569-37573 CD denotes 2000
T20881 37574-37582 NN denotes software
T20885 37583-37586 CC denotes and
T20886 37587-37592 NNP denotes Image
T20887 37593-37594 NNP denotes J
T20888 37595-37603 NN denotes software
T20889 37604-37605 -LRB- denotes (
T20891 37605-37607 NN denotes v.
T20892 37608-37612 CD denotes 1.32
T20893 37612-37613 : denotes ;
T20894 37614-37622 NNP denotes National
T20890 37623-37633 NNPS denotes Institutes
T20895 37634-37636 IN denotes of
T20896 37637-37643 NNP denotes Health
T20897 37643-37644 -RRB- denotes )
T20898 37644-37645 . denotes .
T20899 37645-37725 sentence denotes Colocalization was performed using the overlay coefficient of Image J software.
T20900 37646-37660 NN denotes Colocalization
T20902 37661-37664 VBD denotes was
T20901 37665-37674 VBN denotes performed
T20903 37675-37680 VBG denotes using
T20904 37681-37684 DT denotes the
T20906 37685-37692 JJ denotes overlay
T20905 37693-37704 NN denotes coefficient
T20907 37705-37707 IN denotes of
T20908 37708-37713 NNP denotes Image
T20909 37714-37715 NNP denotes J
T20910 37716-37724 NN denotes software
T20911 37724-37725 . denotes .
R1000 T9121 T9120 amod sixth,domain
R1001 T9005 T9006 compound plasma,levels
R1002 T9122 T9123 npadvmod membrane,spanning
R1003 T9123 T9120 amod spanning,domain
R1004 T9124 T9120 punct ", ",domain
R1005 T9125 T9126 det a,region
R1006 T9006 T9004 nsubj levels,were
R1007 T9126 T9120 appos region,domain
R1008 T9127 T9126 amod rich,region
R1009 T9128 T9127 prep in,rich
R1010 T9129 T9130 amod hydrophobic,acids
R1011 T9130 T9128 pobj acids,in
R1012 T9131 T9130 compound amino,acids
R1013 T9007 T9006 compound glucose,levels
R1014 T9132 T9113 punct .,lies
R1015 T9134 T9135 nsubjpass This,conserved
R1016 T9008 T9004 acomp normal,were
R1017 T9136 T9134 cc and,This
R1018 T9137 T9138 det the,other
R1019 T9009 T9004 cc and,were
R1020 T9138 T9139 nmod other,domains
R1021 T9139 T9134 conj domains,This
R1022 T9140 T9141 npadvmod membrane,spanning
R1023 T9010 T9011 expl there,was
R1024 T9141 T9139 amod spanning,domains
R1025 T9142 T9141 punct -,spanning
R1026 T9011 T9004 conj was,were
R1027 T9143 T9135 auxpass are,conserved
R1028 T9144 T9135 prep among,conserved
R1029 T9145 T9146 amod vertebrate,species
R1030 T9012 T9013 det no,glucose
R1031 T9146 T9144 pobj species,among
R1032 T9147 T9135 punct .,conserved
R1033 T9013 T9011 attr glucose,was
R1034 T9149 T9150 det The,phenylalanine
R1035 T9150 T9151 nsubj phenylalanine,is
R1036 T9014 T9011 prep in,was
R1037 T9152 T9150 prep at,phenylalanine
R1038 T9015 T9016 det the,urine
R1039 T9016 T9014 pobj urine,in
R1040 T9153 T9152 pobj position,at
R1041 T9154 T9153 nummod 204,position
R1042 T9017 T9018 punct (,data
R1043 T9155 T9156 advmod particularly,conserved
R1044 T9156 T9151 acomp conserved,is
R1045 T9018 T9002 meta data,showed
R1046 T9157 T9156 advmod well,conserved
R1047 T9158 T9159 punct (,Figure
R1048 T9159 T9156 parataxis Figure,conserved
R1049 T9019 T9018 amod unpublished,data
R1050 T9160 T9159 nummod 1B,Figure
R1051 T9161 T9159 punct ),Figure
R1052 T9020 T9018 punct ),data
R1053 T9162 T9151 punct ", ",is
R1054 T9163 T9164 preconj not,among
R1055 T9164 T9151 prep among,is
R1056 T9021 T9002 punct .,showed
R1057 T9165 T9163 advmod only,not
R1058 T9166 T9167 amod vertebrate,proteins
R1059 T9023 T9024 advmod Hence,was
R1060 T9167 T9164 pobj proteins,among
R1061 T9168 T9167 compound AQP2,proteins
R1062 T9169 T9164 punct ", ",among
R1063 T9170 T9164 cc but,among
R1064 T9171 T9170 advmod also,but
R1065 T9172 T9164 conj among,among
R1066 T9173 T9174 compound others,members
R1067 T9025 T9024 punct ", ",was
R1068 T9174 T9172 pobj members,among
R1069 T9175 T9174 prep of,members
R1070 T9176 T9177 det this,family
R1071 T9177 T9175 pobj family,of
R1072 T9178 T9151 punct .,is
R1073 T9026 T9024 nsubj this,was
R1074 T9180 T9181 compound Aqp2F204V,F204V
R1075 T9181 T9183 compound F204V,mice
R1076 T9027 T9028 det an,example
R1077 T9182 T9181 punct /,F204V
R1078 T9183 T9184 nsubj mice,have
R1079 T9028 T9024 attr example,was
R1080 T9185 T9186 advmod dramatically,increased
R1081 T9186 T9187 amod increased,production
R1082 T9029 T9028 prep of,example
R1083 T9187 T9184 dobj production,have
R1084 T9188 T9187 compound urine,production
R1085 T9189 T9184 punct ", ",have
R1086 T9030 T9031 compound diabetes,insipidus
R1087 T9190 T9191 prep in,producing
R1088 T9191 T9184 advcl producing,have
R1089 T9031 T9029 pobj insipidus,of
R1090 T9032 T9024 punct .,was
R1091 T9034 T9035 det The,disorder
R1092 T9192 T9193 det some,cases
R1093 T9193 T9190 pobj cases,in
R1094 T9194 T9195 det an,amount
R1095 T9035 T9036 nsubj disorder,segregated
R1096 T9195 T9191 dobj amount,producing
R1097 T9196 T9195 prep of,amount
R1098 T9197 T9196 pobj urine,of
R1099 T9037 T9035 prep in,disorder
R1100 T9198 T9191 prep in,producing
R1101 T9199 T9200 nummod 24,h
R1102 T9200 T9198 pobj h,in
R1103 T9201 T9202 dep that,exceeds
R1104 T9038 T9039 det these,mice
R1105 T9202 T9191 ccomp exceeds,producing
R1106 T9203 T9204 poss their,weight
R1107 T9204 T9202 dobj weight,exceeds
R1108 T9039 T9037 pobj mice,in
R1109 T9205 T9204 compound body,weight
R1110 T9206 T9191 punct ", ",producing
R1111 T9040 T9036 prep in,segregated
R1112 T9207 T9191 prep compared,producing
R1113 T9208 T9207 prep to,compared
R1114 T9209 T9210 poss their,littermates
R1115 T9210 T9208 pobj littermates,to
R1116 T9211 T9210 amod heterozygous,littermates
R1117 T9212 T9211 cc or,heterozygous
R1118 T9041 T9042 det a,manner
R1119 T9213 T9214 amod wild,type
R1120 T9214 T9211 conj type,heterozygous
R1121 T9215 T9214 punct -,type
R1122 T9042 T9040 pobj manner,in
R1123 T9216 T9184 punct .,have
R1124 T9043 T9042 amod monogenic,manner
R1125 T9218 T9219 amod Such,loss
R1126 T9219 T9220 nsubj loss,lead
R1127 T9044 T9042 punct ", ",manner
R1128 T9221 T9219 prep of,loss
R1129 T9222 T9221 pobj water,of
R1130 T9223 T9220 aux would,lead
R1131 T9224 T9220 advmod rapidly,lead
R1132 T9045 T9046 amod autosomal,recessive
R1133 T9225 T9220 prep to,lead
R1134 T9226 T9225 pobj dehydration,to
R1135 T9227 T9228 auxpass were,compensated
R1136 T9046 T9042 amod recessive,manner
R1137 T9228 T9220 advcl compensated,lead
R1138 T9229 T9228 nsubjpass it,compensated
R1139 T9047 T9036 punct ", ",segregated
R1140 T9230 T9228 neg not,compensated
R1141 T9231 T9228 agent by,compensated
R1142 T9232 T9233 amod increased,intake
R1143 T9048 T9036 advcl making,segregated
R1144 T9233 T9231 pobj intake,by
R1145 T9234 T9233 compound water,intake
R1146 T9235 T9220 punct .,lead
R1147 T9049 T9050 nsubj Aqp2,gene
R1148 T9237 T9238 advmod Indeed,increase
R1149 T9050 T9048 ccomp gene,making
R1150 T9239 T9238 punct ", ",increase
R1151 T9051 T9050 det a,gene
R1152 T9240 T9241 compound mutant,mice
R1153 T9052 T9050 compound candidate,gene
R1154 T9241 T9238 nsubj mice,increase
R1155 T9242 T9238 advmod also,increase
R1156 T9053 T9036 punct .,segregated
R1157 T9243 T9238 advmod dramatically,increase
R1158 T9244 T9245 poss their,intake
R1159 T9245 T9238 dobj intake,increase
R1160 T9055 T9056 nsubj Sequencing,identified
R1161 T9246 T9245 compound water,intake
R1162 T9247 T9248 punct (,Figure
R1163 T9248 T9238 parataxis Figure,increase
R1164 T9249 T9248 nummod 1C,Figure
R1165 T9057 T9055 prep of,Sequencing
R1166 T9250 T9248 punct ),Figure
R1167 T9251 T9238 prep compared,increase
R1168 T9252 T9251 prep to,compared
R1169 T9253 T9254 poss their,littermates
R1170 T9254 T9252 pobj littermates,to
R1171 T9058 T9059 compound Aqp2,region
R1172 T9255 T9254 amod heterozygous,littermates
R1173 T9256 T9255 cc or,heterozygous
R1174 T9257 T9258 amod wild,type
R1175 T9059 T9057 pobj region,of
R1176 T9258 T9255 conj type,heterozygous
R1177 T9259 T9258 punct -,type
R1178 T9260 T9238 punct .,increase
R1179 T9060 T9059 compound coding,region
R1180 T9262 T9263 det This,phenotype
R1181 T9061 T9059 prep of,region
R1182 T9263 T9264 nsubj phenotype,showed
R1183 T9265 T9263 punct —,phenotype
R1184 T9062 T9063 amod affected,mice
R1185 T9266 T9267 amod increased,output
R1186 T9267 T9263 appos output,phenotype
R1187 T9268 T9267 amod urinary,output
R1188 T9063 T9061 pobj mice,of
R1189 T9269 T9267 cc and,output
R1190 T9270 T9271 compound water,intake
R1191 T9271 T9267 conj intake,output
R1192 T9064 T9065 det a,transversion
R1193 T9272 T9264 punct —,showed
R1194 T9273 T9274 amod complete,concordance
R1195 T9274 T9264 dobj concordance,showed
R1196 T9065 T9056 dobj transversion,identified
R1197 T9275 T9274 prep with,concordance
R1198 T9276 T9275 pobj homozygosity,with
R1199 T9277 T9276 prep of,homozygosity
R1200 T9066 T9065 nmod thymine,transversion
R1201 T9278 T9279 det the,mutation
R1202 T9279 T9277 pobj mutation,of
R1203 T9280 T9279 compound F204V,mutation
R1204 T9067 T9066 prep to,thymine
R1205 T9281 T9264 prep in,showed
R1206 T9282 T9283 det the,animals
R1207 T9283 T9281 pobj animals,in
R1208 T9068 T9067 pobj guanine,to
R1209 T9284 T9283 nummod 58,animals
R1210 T9285 T9283 acl tested,animals
R1211 T9286 T9264 punct .,showed
R1212 T9069 T9066 punct (,thymine
R1213 T9288 T9289 compound Diabetes,insipidus
R1214 T9289 T9290 nsubjpass insipidus,defined
R1215 T9070 T9066 appos T,thymine
R1216 T9291 T9290 aux can,defined
R1217 T9292 T9290 auxpass be,defined
R1218 T9293 T9290 prep as,defined
R1219 T9294 T9295 det an,inability
R1220 T9295 T9293 pobj inability,as
R1221 T9071 T9070 prep to,T
R1222 T9296 T9297 aux to,concentrate
R1223 T9297 T9295 acl concentrate,inability
R1224 T9072 T9071 pobj G,to
R1225 T9073 T9065 punct ),transversion
R1226 T9074 T9075 punct (,Figure
R1227 T9298 T9297 dobj urine,concentrate
R1228 T9299 T9300 advmod where,appropriate
R1229 T9075 T9065 parataxis Figure,transversion
R1230 T9300 T9297 advcl appropriate,concentrate
R1231 T9301 T9290 punct .,defined
R1232 T9076 T9075 nummod 1A,Figure
R1233 T9303 T9304 prep Compared,produce
R1234 T9305 T9303 prep to,Compared
R1235 T9077 T9075 punct ),Figure
R1236 T9306 T9307 amod wild,type
R1237 T9307 T9309 nmod type,littermates
R1238 T9308 T9307 punct -,type
R1239 T9078 T9065 punct ", ",transversion
R1240 T9309 T9305 pobj littermates,to
R1241 T9310 T9307 cc or,type
R1242 T9311 T9307 conj heterozygous,type
R1243 T9079 T9080 dep which,predicted
R1244 T9312 T9304 punct ", ",produce
R1245 T9313 T9314 compound Aqp2F204V,F204V
R1246 T9314 T9316 compound F204V,mice
R1247 T9080 T9065 relcl predicted,transversion
R1248 T9315 T9314 punct /,F204V
R1249 T9316 T9304 nsubj mice,produce
R1250 T9317 T9318 advmod very,dilute
R1251 T9318 T9319 amod dilute,urine
R1252 T9081 T9080 auxpass is,predicted
R1253 T9319 T9304 dobj urine,produce
R1254 T9320 T9321 punct (,Figure
R1255 T9321 T9304 parataxis Figure,produce
R1256 T9082 T9083 aux to,lead
R1257 T9322 T9321 nummod 1D,Figure
R1258 T9323 T9321 punct ),Figure
R1259 T9083 T9080 xcomp lead,predicted
R1260 T9324 T9304 punct .,produce
R1261 T9326 T9327 amod Basal,concentration
R1262 T9084 T9083 prep to,lead
R1263 T9085 T9086 det a,substitution
R1264 T9327 T9329 nsubj concentration,is
R1265 T9403 T9402 oprd desmopressin,called
R1266 T9328 T9327 compound urine,concentration
R1267 T9330 T9327 prep in,concentration
R1268 T9331 T9332 compound mutant,mice
R1269 T9332 T9330 pobj mice,in
R1270 T9333 T9334 advmod about,161
R1271 T9404 T9383 punct ),analog
R1272 T9334 T9335 nummod 161,mOsm
R1273 T9335 T9329 attr mOsm,is
R1274 T9405 T9386 punct ", ",is
R1275 T9336 T9335 punct ", ",mOsm
R1276 T9337 T9335 prep compared,mOsm
R1277 T9338 T9337 prep to,compared
R1278 T9406 T9407 det a,agonist
R1279 T9339 T9340 quantmod about,"1,293"
R1280 T9340 T9341 nummod "1,293",mOsm
R1281 T9341 T9338 pobj mOsm,to
R1282 T9407 T9386 attr agonist,is
R1283 T9342 T9341 prep in,mOsm
R1284 T9343 T9344 amod wild,type
R1285 T9344 T9346 compound type,mice
R1286 T9408 T9407 amod potent,agonist
R1287 T9345 T9344 punct -,type
R1288 T9346 T9342 pobj mice,in
R1289 T9409 T9407 prep of,agonist
R1290 T9347 T9348 punct (,0.001
R1291 T9348 T9329 parataxis 0.001,is
R1292 T9349 T9348 nsubj p,0.001
R1293 T9410 T9409 pobj AVPR2,of
R1294 T9350 T9348 punct <,0.001
R1295 T9351 T9348 punct ),0.001
R1296 T9352 T9329 punct .,is
R1297 T9411 T9386 punct .,is
R1298 T9354 T9355 advmod Normally,is
R1299 T9413 T9414 advmod When,administered
R1300 T9356 T9355 punct ", ",is
R1301 T9357 T9358 compound urine,concentration
R1302 T9358 T9355 nsubj concentration,is
R1303 T9359 T9355 prep under,is
R1304 T9360 T9361 det the,control
R1305 T9361 T9359 pobj control,under
R1306 T9414 T9415 advcl administered,leads
R1307 T9362 T9361 prep of,control
R1308 T9363 T9364 det the,hypothalamus
R1309 T9364 T9362 pobj hypothalamus,of
R1310 T9365 T9364 punct ", ",hypothalamus
R1311 T9416 T9414 prep to,administered
R1312 T9366 T9367 dep which,secretes
R1313 T9367 T9364 relcl secretes,hypothalamus
R1314 T9368 T9367 punct ", ",secretes
R1315 T9369 T9367 prep in,secretes
R1316 T9417 T9418 amod wild,type
R1317 T9370 T9369 pobj response,in
R1318 T9371 T9370 prep to,response
R1319 T9372 T9371 pobj hypovolemia,to
R1320 T9373 T9372 cc or,hypovolemia
R1321 T9374 T9372 conj hypernatremia,hypovolemia
R1322 T9375 T9376 punct [,21
R1323 T9418 T9420 compound type,mice
R1324 T9376 T9370 parataxis 21,response
R1325 T9377 T9376 punct ],21
R1326 T9378 T9367 punct ", ",secretes
R1327 T9379 T9367 dobj AVP,secretes
R1328 T9419 T9418 punct -,type
R1329 T9380 T9355 punct .,is
R1330 T9382 T9383 det The,analog
R1331 T9420 T9416 pobj mice,to
R1332 T9383 T9386 nsubj analog,is
R1333 T9384 T9383 amod synthetic,analog
R1334 T9421 T9415 punct ", ",leads
R1335 T9385 T9383 compound AVP,analog
R1336 T9387 T9383 punct ", ",analog
R1337 T9422 T9415 nsubj dDAVP,leads
R1338 T9388 T9389 nummod 1,deamino
R1339 T9389 T9391 nmod deamino,arginine
R1340 T9390 T9389 punct -,deamino
R1341 T9423 T9415 prep to,leads
R1342 T9424 T9425 det a,increase
R1343 T9391 T9397 compound arginine,vasopressin
R1344 T9425 T9423 pobj increase,to
R1345 T9392 T9391 punct -,arginine
R1346 T9393 T9391 nummod 8,arginine
R1347 T9426 T9425 amod dramatic,increase
R1348 T9394 T9391 punct -,arginine
R1349 T9395 T9391 compound D,arginine
R1350 T9396 T9391 punct -,arginine
R1351 T9427 T9425 prep in,increase
R1352 T9397 T9383 appos vasopressin,analog
R1353 T9398 T9397 punct (,vasopressin
R1354 T9399 T9397 appos dDAVP,vasopressin
R1355 T9428 T9429 compound urine,concentration
R1356 T9400 T9397 punct ;,vasopressin
R1357 T9401 T9402 advmod also,called
R1358 T9402 T9397 acl called,vasopressin
R1359 T9429 T9427 pobj concentration,in
R1360 T9430 T9425 punct ", ",increase
R1361 T9431 T9425 prep from,increase
R1362 T9432 T9431 pobj "1,293",from
R1363 T9509 T9510 dep which,be
R1364 T9510 T9505 relcl be,channel
R1365 T9433 T9431 prep to,from
R1366 T9511 T9510 aux must,be
R1367 T9512 T9510 acomp sufficient,be
R1368 T9513 T9514 aux to,allow
R1369 T9434 T9433 pobj "5,885",to
R1370 T9514 T9512 xcomp allow,sufficient
R1371 T9515 T9514 dobj survival,allow
R1372 T9516 T9515 prep of,survival
R1373 T9517 T9518 det the,individual
R1374 T9518 T9516 pobj individual,of
R1375 T9519 T9497 punct ", ",indicates
R1376 T9435 T9431 pobj mOsm,from
R1377 T9520 T9497 prep in,indicates
R1378 T9521 T9520 pobj contrast,in
R1379 T9522 T9521 prep to,contrast
R1380 T9436 T9437 punct (,Figure
R1381 T9523 T9524 det the,mouse
R1382 T9524 T9522 pobj mouse,to
R1383 T9437 T9415 parataxis Figure,leads
R1384 T9525 T9524 nmod T126M,mouse
R1385 T9526 T9524 amod knock,mouse
R1386 T9527 T9526 punct -,knock
R1387 T9528 T9526 prt in,knock
R1388 T9438 T9437 advmod 4.6-fold,Figure
R1389 T9529 T9530 punct [,18
R1390 T9530 T9497 parataxis 18,indicates
R1391 T9531 T9530 punct ],18
R1392 T9439 T9437 punct ;,Figure
R1393 T9532 T9497 punct .,indicates
R1394 T9534 T9535 amod Multiple,matings
R1395 T9440 T9437 nummod 1D,Figure
R1396 T9535 T9537 nsubj matings,yielded
R1397 T9536 T9535 amod heterozygous,matings
R1398 T9441 T9437 punct ),Figure
R1399 T9538 T9539 nummod 101,animals
R1400 T9539 T9537 dobj animals,yielded
R1401 T9442 T9415 punct .,leads
R1402 T9540 T9539 punct ", ",animals
R1403 T9541 T9542 dep which,appeared
R1404 T9542 T9539 relcl appeared,animals
R1405 T9543 T9542 prep at,appeared
R1406 T9444 T9445 prep With,concentrate
R1407 T9544 T9545 det a,ratio
R1408 T9545 T9543 pobj ratio,at
R1409 T9546 T9545 prep of,ratio
R1410 T9547 T9546 pobj 26,of
R1411 T9446 T9447 amod similar,treatment
R1412 T9548 T9549 punct :,49
R1413 T9549 T9547 prep 49,26
R1414 T9550 T9551 punct :,26
R1415 T9447 T9444 pobj treatment,With
R1416 T9551 T9547 prep 26,26
R1417 T9552 T9542 punct ", ",appeared
R1418 T9553 T9542 prep near,appeared
R1419 T9448 T9445 punct ", ",concentrate
R1420 T9554 T9555 det the,frequencies
R1421 T9555 T9553 pobj frequencies,near
R1422 T9556 T9555 amod expected,frequencies
R1423 T9557 T9558 amod Mendelian,type
R1424 T9558 T9555 nmod type,frequencies
R1425 T9559 T9558 amod wild,type
R1426 T9449 T9450 compound mutant,mice
R1427 T9560 T9558 punct ", ",type
R1428 T9561 T9558 conj heterozygote,type
R1429 T9562 T9561 punct ", ",heterozygote
R1430 T9450 T9445 nsubj mice,concentrate
R1431 T9563 T9561 cc and,heterozygote
R1432 T9564 T9561 conj mutant,heterozygote
R1433 T9565 T9542 punct ", ",appeared
R1434 T9451 T9452 poss their,urine
R1435 T9566 T9542 advmod respectively,appeared
R1436 T9567 T9537 punct ", ",yielded
R1437 T9452 T9445 dobj urine,concentrate
R1438 T9568 T9537 advcl indicating,yielded
R1439 T9569 T9570 mark that,is
R1440 T9570 T9568 ccomp is,indicating
R1441 T9571 T9570 expl there,is
R1442 T9453 T9445 prep to,concentrate
R1443 T9572 T9573 det no,viability
R1444 T9573 T9570 attr viability,is
R1445 T9454 T9455 det a,extent
R1446 T9574 T9573 amod reduced,viability
R1447 T9575 T9573 acl associated,viability
R1448 T9576 T9575 prep with,associated
R1449 T9455 T9453 pobj extent,to
R1450 T9577 T9578 det this,mutation
R1451 T9578 T9576 pobj mutation,with
R1452 T9579 T9537 punct .,yielded
R1453 T9456 T9455 amod lesser,extent
R1454 T9581 T9582 amod Other,was
R1455 T9457 T9456 cc but,lesser
R1456 T9583 T9581 prep than,Other
R1457 T9458 T9459 advmod still,significant
R1458 T9459 T9456 conj significant,lesser
R1459 T9584 T9585 det the,production
R1460 T9585 T9583 pobj production,than
R1461 T9586 T9585 amod increased,production
R1462 T9460 T9445 punct ", ",concentrate
R1463 T9587 T9585 compound urine,production
R1464 T9588 T9585 cc and,production
R1465 T9589 T9590 compound water,intake
R1466 T9461 T9445 prep from,concentrate
R1467 T9590 T9585 conj intake,production
R1468 T9591 T9582 punct ", ",was
R1469 T9592 T9582 expl there,was
R1470 T9462 T9461 pobj 161,from
R1471 T9593 T9594 det no,phenotype
R1472 T9594 T9582 attr phenotype,was
R1473 T9463 T9461 prep to,from
R1474 T9595 T9594 amod overt,phenotype
R1475 T9596 T9594 prep in,phenotype
R1476 T9597 T9598 compound mutant,mice
R1477 T9598 T9596 pobj mice,in
R1478 T9599 T9594 punct ", ",phenotype
R1479 T9600 T9594 prep save,phenotype
R1480 T9464 T9463 pobj 470,to
R1481 T9601 T9602 amod distended,kidneys
R1482 T9602 T9600 pobj kidneys,save
R1483 T9603 T9602 punct ", ",kidneys
R1484 T9465 T9461 pobj mOsm,from
R1485 T9604 T9605 dep which,appeared
R1486 T9605 T9602 relcl appeared,kidneys
R1487 T9606 T9605 advmod variably,appeared
R1488 T9466 T9467 punct (,2.9-fold
R1489 T9607 T9605 prep in,appeared
R1490 T9608 T9609 amod adult,animals
R1491 T9609 T9607 pobj animals,in
R1492 T9467 T9461 parataxis 2.9-fold,from
R1493 T9610 T9611 punct (,Figure
R1494 T9611 T9582 parataxis Figure,was
R1495 T9612 T9611 nummod 2A,Figure
R1496 T9468 T9467 punct ),2.9-fold
R1497 T9613 T9611 punct ),Figure
R1498 T9614 T9582 punct .,was
R1499 T9469 T9445 punct ", ",concentrate
R1500 T9470 T9445 advcl indicating,concentrate
R1501 T9471 T9472 mark that,are
R1502 T9616 T9617 mark Although,measured
R1503 T9472 T9470 ccomp are,indicating
R1504 T9617 T9620 advcl measured,seem
R1505 T9618 T9617 neg not,measured
R1506 T9619 T9617 advmod specifically,measured
R1507 T9473 T9474 det these,animals
R1508 T9621 T9620 punct ", ",seem
R1509 T9622 T9623 compound mutant,mice
R1510 T9474 T9472 nsubj animals,are
R1511 T9623 T9620 nsubj mice,seem
R1512 T9624 T9625 aux to,have
R1513 T9625 T9620 xcomp have,seem
R1514 T9475 T9472 preconj not,are
R1515 T9626 T9627 det a,lifespan
R1516 T9627 T9625 dobj lifespan,have
R1517 T9628 T9627 amod normal,lifespan
R1518 T9476 T9475 advmod only,not
R1519 T9629 T9620 punct .,seem
R1520 T9477 T9472 conj unable,are
R1521 T9631 T9632 det The,animal
R1522 T9632 T9634 nsubj animal,lived
R1523 T9633 T9632 nummod one,animal
R1524 T9478 T9479 aux to,concentrate
R1525 T9635 T9636 dep that,followed
R1526 T9636 T9632 relcl followed,animal
R1527 T9637 T9636 auxpass was,followed
R1528 T9638 T9634 prep to,lived
R1529 T9639 T9640 nummod 18,mo
R1530 T9479 T9477 xcomp concentrate,unable
R1531 T9640 T9638 pobj mo,to
R1532 T9641 T9634 punct ", ",lived
R1533 T9642 T9634 advcl typical,lived
R1534 T9480 T9481 poss their,urine
R1535 T9643 T9642 prep for,typical
R1536 T9644 T9643 pobj animals,for
R1537 T9645 T9644 prep in,animals
R1538 T9481 T9479 dobj urine,concentrate
R1539 T9646 T9647 poss our,colony
R1540 T9647 T9645 pobj colony,in
R1541 T9648 T9634 punct .,lived
R1542 T9482 T9479 advmod properly,concentrate
R1543 T9650 T9651 compound Aqp2F204V,F204V
R1544 T9651 T9653 compound F204V,mice
R1545 T9483 T9472 cc but,are
R1546 T9652 T9651 punct /,F204V
R1547 T9653 T9654 nsubj mice,suffer
R1548 T9484 T9472 conj are,are
R1549 T9655 T9654 prep from,suffer
R1550 T9656 T9657 amod severe,hydronephrosis
R1551 T9485 T9484 advmod also,are
R1552 T9657 T9655 pobj hydronephrosis,from
R1553 T9658 T9659 punct (,2A
R1554 T9486 T9484 acomp defective,are
R1555 T9659 T9654 parataxis 2A,suffer
R1556 T9660 T9659 nmod Figure,2A
R1557 T9661 T9659 cc and,2A
R1558 T9487 T9484 prep in,are
R1559 T9662 T9659 conj 2B,2A
R1560 T9663 T9659 punct ),2A
R1561 T9664 T9654 punct ", ",suffer
R1562 T9488 T9489 poss their,response
R1563 T9665 T9666 advmod presumably,as
R1564 T9666 T9654 prep as,suffer
R1565 T9667 T9668 det a,consequence
R1566 T9489 T9487 pobj response,in
R1567 T9668 T9666 pobj consequence,as
R1568 T9669 T9668 prep of,consequence
R1569 T9490 T9489 prep to,response
R1570 T9491 T9490 pobj dDAVP,to
R1571 T9670 T9671 det an,inability
R1572 T9492 T9445 punct .,concentrate
R1573 T9671 T9669 pobj inability,of
R1574 T9672 T9673 aux to,cope
R1575 T9673 T9671 acl cope,inability
R1576 T9494 T9495 det The,response
R1577 T9674 T9673 prep with,cope
R1578 T9675 T9676 det the,polyuria
R1579 T9676 T9674 pobj polyuria,with
R1580 T9677 T9676 amod extreme,polyuria
R1581 T9678 T9654 punct .,suffer
R1582 T9495 T9497 nsubj response,indicates
R1583 T9680 T9681 nsubj We,found
R1584 T9681 T9682 ccomp found,was
R1585 T9496 T9495 amod smaller,response
R1586 T9683 T9684 amod distended,kidneys
R1587 T9684 T9681 dobj kidneys,found
R1588 T9685 T9681 prep in,found
R1589 T9498 T9495 prep to,response
R1590 T9686 T9687 det all,mice
R1591 T9687 T9685 pobj mice,in
R1592 T9688 T9689 compound Aqp2F204V,F204V
R1593 T9689 T9687 compound F204V,mice
R1594 T9499 T9498 pobj dDAVP,to
R1595 T9690 T9689 punct /,F204V
R1596 T9691 T9682 punct ;,was
R1597 T9692 T9682 advmod however,was
R1598 T9500 T9501 det some,activity
R1599 T9693 T9682 punct ", ",was
R1600 T9694 T9695 det the,degree
R1601 T9695 T9682 nsubj degree,was
R1602 T9501 T9497 dobj activity,indicates
R1603 T9696 T9695 prep of,degree
R1604 T9697 T9696 pobj inflation,of
R1605 T9502 T9501 amod residual,activity
R1606 T9698 T9682 acomp variable,was
R1607 T9699 T9682 prep in,was
R1608 T9700 T9701 amod affected,mice
R1609 T9503 T9501 prep of,activity
R1610 T9701 T9699 pobj mice,in
R1611 T9702 T9682 cc and,was
R1612 T9703 T9682 conj worsened,was
R1613 T9704 T9703 prep with,worsened
R1614 T9504 T9505 det the,channel
R1615 T9705 T9704 pobj age,with
R1616 T9706 T9682 punct .,was
R1617 T9505 T9503 pobj channel,of
R1618 T9708 T9709 amod Severe,hydronephrosis
R1619 T9709 T9710 nsubjpass hydronephrosis,observed
R1620 T9506 T9505 compound mutant,channel
R1621 T9711 T9710 aux has,observed
R1622 T9712 T9710 advmod previously,observed
R1623 T9507 T9505 compound AQP2,channel
R1624 T9713 T9710 auxpass been,observed
R1625 T9714 T9710 prep in,observed
R1626 T9715 T9716 amod double,knock
R1627 T9508 T9505 punct ", ",channel
R1628 T9716 T9720 amod knock,mice
R1629 T9717 T9718 compound Aqp1,Aqp3
R1630 T9718 T9716 npadvmod Aqp3,knock
R1631 T9719 T9718 punct /,Aqp3
R1632 T9720 T9714 pobj mice,in
R1633 T9827 T9828 det the,features
R1634 T9721 T9716 punct -,knock
R1635 T9828 T9825 nsubj features,were
R1636 T9722 T9716 prt out,knock
R1637 T9723 T9724 punct [,17
R1638 T9829 T9828 amod morphologic,features
R1639 T9724 T9710 parataxis 17,observed
R1640 T9725 T9724 punct ],17
R1641 T9726 T9710 punct ", ",observed
R1642 T9830 T9828 prep of,features
R1643 T9727 T9710 cc and,observed
R1644 T9728 T9710 conj appears,observed
R1645 T9729 T9728 prep at,appears
R1646 T9831 T9832 det the,glomeruli
R1647 T9730 T9731 nummod 6,wk
R1648 T9731 T9729 pobj wk,at
R1649 T9732 T9710 punct .,observed
R1650 T9832 T9830 pobj glomeruli,of
R1651 T9734 T9735 advmod Even,at
R1652 T9833 T9832 cc and,glomeruli
R1653 T9735 T9736 prep at,had
R1654 T9737 T9738 nummod 4,wk
R1655 T9834 T9835 amod proximal,distal
R1656 T9738 T9735 pobj wk,at
R1657 T9739 T9736 punct ", ",had
R1658 T9835 T9837 amod distal,tubules
R1659 T9740 T9741 compound Aqp2F204V,F204V
R1660 T9741 T9743 compound F204V,mice
R1661 T9742 T9741 punct /,F204V
R1662 T9743 T9736 nsubj mice,had
R1663 T9836 T9835 punct /,distal
R1664 T9744 T9736 dobj hydronephrosis,had
R1665 T9745 T9736 punct .,had
R1666 T9837 T9832 conj tubules,glomeruli
R1667 T9747 T9748 amod Histologic,sections
R1668 T9748 T9749 nsubj sections,demonstrated
R1669 T9838 T9825 acomp unremarkable,were
R1670 T9750 T9748 prep from,sections
R1671 T9751 T9752 compound Aqp2F204V,F204V
R1672 T9839 T9840 punct (,Figure
R1673 T9752 T9754 compound F204V,mice
R1674 T9753 T9752 punct /,F204V
R1675 T9754 T9750 pobj mice,from
R1676 T9840 T9825 parataxis Figure,were
R1677 T9755 T9756 amod marked,dilatation
R1678 T9756 T9749 dobj dilatation,demonstrated
R1679 T9841 T9840 nummod 2D,Figure
R1680 T9757 T9756 prep of,dilatation
R1681 T9758 T9759 det the,pelvis
R1682 T9842 T9840 punct ),Figure
R1683 T9759 T9757 pobj pelvis,of
R1684 T9760 T9759 amod renal,pelvis
R1685 T9761 T9756 cc yet,dilatation
R1686 T9762 T9763 amod normal,morphology
R1687 T9843 T9825 punct .,were
R1688 T9763 T9756 conj morphology,dilatation
R1689 T9764 T9763 prep of,morphology
R1690 T9765 T9766 det the,ureter
R1691 T9845 T9846 mark As,shown
R1692 T9766 T9764 pobj ureter,of
R1693 T9767 T9768 punct (,2C
R1694 T9768 T9749 parataxis 2C,demonstrated
R1695 T9846 T9847 advcl shown,revealed
R1696 T9769 T9768 nmod Figure,2C
R1697 T9770 T9768 cc and,2C
R1698 T9771 T9768 conj 2D,2C
R1699 T9848 T9846 advmod previously,shown
R1700 T9772 T9768 punct ),2C
R1701 T9773 T9749 punct .,demonstrated
R1702 T9849 T9850 punct [,22
R1703 T9775 T9776 prep In,was
R1704 T9777 T9775 amod particular,In
R1705 T9778 T9776 punct ", ",was
R1706 T9779 T9780 det the,propria
R1707 T9850 T9846 parataxis 22,shown
R1708 T9780 T9776 nsubj propria,was
R1709 T9781 T9780 compound muscularis,propria
R1710 T9782 T9783 preconj neither,hypertrophied
R1711 T9851 T9850 nummod 18,22
R1712 T9852 T9850 punct ",",22
R1713 T9783 T9776 acomp hypertrophied,was
R1714 T9853 T9850 punct ],22
R1715 T9784 T9783 cc nor,hypertrophied
R1716 T9785 T9783 conj thinned,hypertrophied
R1717 T9854 T9847 punct ", ",revealed
R1718 T9786 T9776 punct .,was
R1719 T9855 T9847 nsubj immunoblotting,revealed
R1720 T9788 T9789 expl There,was
R1721 T9790 T9791 det the,appearance
R1722 T9856 T9857 nummod three,forms
R1723 T9791 T9789 attr appearance,was
R1724 T9792 T9791 amod normal,appearance
R1725 T9857 T9847 dobj forms,revealed
R1726 T9793 T9791 amod festooned,appearance
R1727 T9794 T9791 prep of,appearance
R1728 T9795 T9796 det the,urothelium
R1729 T9796 T9794 pobj urothelium,of
R1730 T9858 T9857 amod different,forms
R1731 T9797 T9789 punct ", ",was
R1732 T9798 T9789 cc and,was
R1733 T9859 T9857 prep of,forms
R1734 T9799 T9800 det this,epithelium
R1735 T9800 T9802 nsubj epithelium,was
R1736 T9801 T9800 amod transitional,epithelium
R1737 T9802 T9789 conj was,was
R1738 T9803 T9802 prep of,was
R1739 T9804 T9805 amod normal,thickness
R1740 T9860 T9859 pobj AQP2,of
R1741 T9805 T9803 pobj thickness,of
R1742 T9806 T9802 punct .,was
R1743 T9861 T9847 punct ", ",revealed
R1744 T9808 T9809 expl There,was
R1745 T9810 T9809 attr thinning,was
R1746 T9862 T9847 prep due,revealed
R1747 T9811 T9810 prep of,thinning
R1748 T9812 T9813 det the,kidney
R1749 T9813 T9811 pobj kidney,of
R1750 T9863 T9862 pcomp to,due
R1751 T9814 T9815 mark as,measured
R1752 T9815 T9809 advcl measured,was
R1753 T9816 T9815 prep from,measured
R1754 T9864 T9865 amod different,degrees
R1755 T9817 T9818 amod renal,capsule
R1756 T9818 T9816 pobj capsule,from
R1757 T9819 T9816 prep to,from
R1758 T9865 T9862 pobj degrees,due
R1759 T9820 T9821 amod renal,pelvis
R1760 T9821 T9819 pobj pelvis,to
R1761 T9866 T9865 cc and,degrees
R1762 T9822 T9809 punct .,was
R1763 T9824 T9825 advmod However,were
R1764 T9867 T9865 conj forms,degrees
R1765 T9826 T9825 punct ", ",were
R1766 T9868 T9865 prep of,degrees
R1767 T9869 T9868 pobj glycosylation,of
R1768 T9870 T9871 punct (,Figure
R1769 T9871 T9847 parataxis Figure,revealed
R1770 T9872 T9871 nummod 3A,Figure
R1771 T9933 T9932 punct ],22
R1772 T9873 T9871 punct ),Figure
R1773 T9934 T9911 punct .,is
R1774 T9874 T9847 punct .,revealed
R1775 T9936 T9937 prep Compared,reduced
R1776 T9876 T9877 amod Previous,reports
R1777 T9938 T9936 prep to,Compared
R1778 T9939 T9938 pobj that,to
R1779 T9940 T9939 prep from,that
R1780 T9941 T9942 det the,kidneys
R1781 T9877 T9878 nsubj reports,demonstrated
R1782 T9942 T9940 pobj kidneys,from
R1783 T9943 T9942 prep of,kidneys
R1784 T9879 T9878 aux have,demonstrated
R1785 T9944 T9945 amod wild,type
R1786 T9945 T9947 compound type,animals
R1787 T9946 T9945 punct -,type
R1788 T9947 T9943 pobj animals,of
R1789 T9948 T9937 punct ", ",reduced
R1790 T9949 T9937 nsubjpass AQP2,reduced
R1791 T9880 T9881 mark that,appears
R1792 T9950 T9949 prep from,AQP2
R1793 T9951 T9952 compound mutant,animals
R1794 T9952 T9950 pobj animals,from
R1795 T9881 T9878 ccomp appears,demonstrated
R1796 T9953 T9937 auxpass was,reduced
R1797 T9954 T9937 prep in,reduced
R1798 T9955 T9956 preconj both,form
R1799 T9882 T9883 amod nonglycosylated,protein
R1800 T9956 T9954 pobj form,in
R1801 T9957 T9956 det the,form
R1802 T9883 T9881 nsubj protein,appears
R1803 T9958 T9959 amod high,weight
R1804 T9959 T9956 nmod weight,form
R1805 T9960 T9959 amod molecular,weight
R1806 T9884 T9881 prep as,appears
R1807 T9961 T9956 punct ", ",form
R1808 T9962 T9956 amod diffuse,form
R1809 T9963 T9956 cc and,form
R1810 T9885 T9886 det a,band
R1811 T9964 T9965 det the,form
R1812 T9965 T9956 conj form,form
R1813 T9966 T9967 amod lowest,weight
R1814 T9886 T9884 pobj band,as
R1815 T9967 T9965 compound weight,form
R1816 T9968 T9967 amod molecular,weight
R1817 T9969 T9937 punct ", ",reduced
R1818 T9887 T9888 nummod 29,kDa
R1819 T9970 T9937 cc but,reduced
R1820 T9971 T9937 conj enriched,reduced
R1821 T9888 T9886 compound kDa,band
R1822 T9972 T9971 prep in,enriched
R1823 T9973 T9974 det the,form
R1824 T9974 T9972 pobj form,in
R1825 T9889 T9881 punct ", ",appears
R1826 T9975 T9976 amod intermediate,weight
R1827 T9976 T9974 compound weight,form
R1828 T9977 T9976 amod molecular,weight
R1829 T9890 T9891 mark while,runs
R1830 T9978 T9979 punct (,Figure
R1831 T9979 T9971 parataxis Figure,enriched
R1832 T9980 T9979 nummod 3A,Figure
R1833 T9891 T9881 advcl runs,appears
R1834 T9981 T9979 punct ),Figure
R1835 T9982 T9937 punct .,reduced
R1836 T9892 T9893 amod complex,protein
R1837 T9984 T9985 amod Heterozygous,animals
R1838 T9985 T9986 nsubj animals,showed
R1839 T9987 T9988 amod intermediate,amounts
R1840 T9988 T9986 dobj amounts,showed
R1841 T9893 T9891 nsubj protein,runs
R1842 T9989 T9988 prep of,amounts
R1843 T9990 T9991 det all,forms
R1844 T9991 T9989 pobj forms,of
R1845 T9894 T9893 amod glycosylated,protein
R1846 T9992 T9991 nummod three,forms
R1847 T9993 T9986 punct .,showed
R1848 T9895 T9891 prep as,runs
R1849 T9995 T9996 det The,nature
R1850 T9996 T9997 nsubjpass nature,revealed
R1851 T9896 T9897 det a,smear
R1852 T9998 T9996 prep of,nature
R1853 T9999 T10000 det these,forms
R1854 T9897 T9895 pobj smear,as
R1855 T10000 T9998 pobj forms,of
R1856 T10001 T10000 amod glycosylated,forms
R1857 T9898 T9897 prep between,smear
R1858 T10002 T9997 auxpass was,revealed
R1859 T10003 T9997 prep by,revealed
R1860 T10004 T10003 pobj digestion,by
R1861 T9899 T9900 nummod 35,kDa
R1862 T10005 T10004 prep with,digestion
R1863 T10006 T10007 compound endoglycosidase,H
R1864 T10007 T10005 pobj H,with
R1865 T9900 T9898 pobj kDa,between
R1866 T10008 T10007 punct ", ",H
R1867 T10009 T10010 dep which,cleaves
R1868 T9901 T9899 cc and,35
R1869 T10010 T10007 relcl cleaves,H
R1870 T10011 T10010 advmod specifically,cleaves
R1871 T10012 T10013 npadvmod mannose,rich
R1872 T9902 T9899 conj 45,35
R1873 T10013 T10015 amod rich,carbohydrate
R1874 T10014 T10013 punct -,rich
R1875 T10015 T10010 dobj carbohydrate,cleaves
R1876 T9903 T9878 punct .,demonstrated
R1877 T10016 T10010 prep from,cleaves
R1878 T10017 T10018 det the,backbone
R1879 T10018 T10016 pobj backbone,from
R1880 T9905 T9906 det A,form
R1881 T10019 T10018 compound protein,backbone
R1882 T9906 T9911 nsubj form,is
R1883 T10020 T9997 punct .,revealed
R1884 T9907 T9908 amod short,lived
R1885 T10022 T10023 nsubj Treatment,affected
R1886 T9908 T9906 amod lived,form
R1887 T10024 T10022 prep of,Treatment
R1888 T10025 T10026 amod endogenous,AQP2
R1889 T10026 T10024 pobj AQP2,of
R1890 T9909 T9908 punct -,lived
R1891 T10027 T10026 prep from,AQP2
R1892 T10028 T10027 pobj kidneys,from
R1893 T10029 T10028 prep of,kidneys
R1894 T10030 T10031 amod wild,type
R1895 T10031 T10033 nmod type,animals
R1896 T9910 T9906 amod intermediate,form
R1897 T10032 T10031 punct -,type
R1898 T10033 T10029 pobj animals,of
R1899 T9912 T9906 prep of,form
R1900 T10034 T10031 punct ", ",type
R1901 T10035 T10031 conj heterozygous,type
R1902 T10036 T10035 punct ", ",heterozygous
R1903 T10037 T10035 cc and,heterozygous
R1904 T9913 T9914 nummod 31,kDa
R1905 T10038 T10035 conj mutant,heterozygous
R1906 T9914 T9912 pobj kDa,of
R1907 T9915 T9906 acl representing,form
R1908 T9916 T9917 nmod core,glycosylation
R1909 T10039 T10023 advmod specifically,affected
R1910 T10040 T10041 det the,form
R1911 T9917 T9915 dobj glycosylation,representing
R1912 T10041 T10023 dobj form,affected
R1913 T10042 T10043 amod intermediate,weight
R1914 T10043 T10041 compound weight,form
R1915 T9918 T9917 punct ", ",glycosylation
R1916 T10044 T10043 amod molecular,weight
R1917 T10045 T10046 punct (,Figure
R1918 T9919 T9920 amod high,mannose
R1919 T10046 T10023 parataxis Figure,affected
R1920 T10047 T10046 nummod 3B,Figure
R1921 T10048 T10046 punct ),Figure
R1922 T9920 T9917 compound mannose,glycosylation
R1923 T10049 T10023 punct .,affected
R1924 T10051 T10052 det The,presence
R1925 T9921 T9920 punct -,mannose
R1926 T10052 T10053 nsubj presence,permits
R1927 T9922 T9917 prep of,glycosylation
R1928 T10054 T10052 prep of,presence
R1929 T10055 T10056 det some,proteins
R1930 T10056 T10054 pobj proteins,of
R1931 T9923 T9922 pobj AQP2,of
R1932 T10057 T10056 amod mature,proteins
R1933 T10058 T10056 amod glycosylated,proteins
R1934 T10059 T10060 punct (,kDa
R1935 T9924 T9911 acomp apparent,is
R1936 T10060 T10056 parataxis kDa,proteins
R1937 T10061 T10062 quantmod 35,45
R1938 T10062 T10060 nummod 45,kDa
R1939 T9925 T9924 prep from,apparent
R1940 T10063 T10062 punct –,45
R1941 T10064 T10060 punct ),kDa
R1942 T9926 T9927 compound pulse,chase
R1943 T10065 T10056 prep in,proteins
R1944 T10066 T10067 compound Aqp2F204V,F204V
R1945 T10067 T10069 compound F204V,mice
R1946 T10068 T10067 punct /,F204V
R1947 T10069 T10065 pobj mice,in
R1948 T10070 T10053 advmod presumably,permits
R1949 T9927 T9929 compound chase,labeling
R1950 T10071 T10072 poss their,survival
R1951 T10072 T10053 dobj survival,permits
R1952 T10073 T10072 prep compared,survival
R1953 T9928 T9927 punct -,chase
R1954 T10074 T10073 prep to,compared
R1955 T10075 T10076 compound Aqp2T126M,T126M
R1956 T10076 T10078 compound T126M,mice
R1957 T9929 T9930 compound labeling,experiments
R1958 T10077 T10076 punct /,T126M
R1959 T10078 T10074 pobj mice,to
R1960 T10079 T10053 punct ", ",permits
R1961 T9930 T9925 pobj experiments,from
R1962 T10080 T10053 cc and,permits
R1963 T10081 T10053 conj is,permits
R1964 T10082 T10081 acomp consistent,is
R1965 T9931 T9932 punct [,22
R1966 T10083 T10082 prep with,consistent
R1967 T10084 T10085 det a,response
R1968 T9932 T9911 parataxis 22,is
R1969 T10085 T10083 pobj response,with
R1970 T10086 T10085 amod diminished,response
R1971 T10087 T10085 prep to,response
R1972 T10088 T10087 pobj dDAVP,to
R1973 T10146 T10147 prep In,fail
R1974 T10089 T10053 punct .,permits
R1975 T10091 T10092 prep In,postulated
R1976 T10093 T10091 pobj humans,In
R1977 T10148 T10146 amod general,In
R1978 T10094 T10092 punct ", ",postulated
R1979 T10095 T10096 amod recessive,alleles
R1980 T10096 T10092 nsubjpass alleles,postulated
R1981 T10097 T10096 prep of,alleles
R1982 T10149 T10147 punct ", ",fail
R1983 T10098 T10097 pobj Aqp2,of
R1984 T10099 T10092 auxpass are,postulated
R1985 T10100 T10101 aux to,cause
R1986 T10150 T10151 amod such,alleles
R1987 T10101 T10092 xcomp cause,postulated
R1988 T10102 T10101 dobj NDI,cause
R1989 T10103 T10104 mark because,translocate
R1990 T10151 T10147 nsubj alleles,fail
R1991 T10152 T10151 amod recessive,alleles
R1992 T10104 T10101 advcl translocate,cause
R1993 T10105 T10104 nsubj they,translocate
R1994 T10153 T10147 punct ", ",fail
R1995 T10106 T10104 aux do,translocate
R1996 T10107 T10104 neg not,translocate
R1997 T10108 T10104 advmod properly,translocate
R1998 T10109 T10104 prep to,translocate
R1999 T10110 T10111 det the,surface
R2000 T10154 T10155 advmod when,visualized
R2001 T10111 T10109 pobj surface,to
R2002 T10112 T10111 amod apical,surface
R2003 T10113 T10111 compound cell,surface
R2004 T10114 T10104 prep in,translocate
R2005 T10155 T10147 advcl visualized,fail
R2006 T10115 T10114 pobj response,in
R2007 T10116 T10115 prep to,response
R2008 T10156 T10155 advmod immunocytochemically,visualized
R2009 T10117 T10116 pobj AVP,to
R2010 T10118 T10092 punct .,postulated
R2011 T10157 T10147 punct ", ",fail
R2012 T10120 T10121 det This,postulate
R2013 T10121 T10122 nsubj postulate,comes
R2014 T10158 T10159 aux to,localize
R2015 T10123 T10124 advmod solely,from
R2016 T10124 T10122 prep from,comes
R2017 T10159 T10147 xcomp localize,fail
R2018 T10125 T10126 advmod in,vitro
R2019 T10126 T10127 amod vitro,studies
R2020 T10127 T10124 pobj studies,from
R2021 T10160 T10159 prep to,localize
R2022 T10128 T10129 prep in,transfected
R2023 T10129 T10127 relcl transfected,studies
R2024 T10130 T10128 pobj which,in
R2025 T10161 T10162 npadvmod AVP,responsive
R2026 T10131 T10132 compound mutant,cDNAs
R2027 T10132 T10129 nsubjpass cDNAs,transfected
R2028 T10133 T10132 compound Aqp2,cDNAs
R2029 T10162 T10164 amod responsive,vesicles
R2030 T10134 T10132 acl corresponding,cDNAs
R2031 T10135 T10134 prep to,corresponding
R2032 T10136 T10137 amod human,mutations
R2033 T10163 T10162 punct -,responsive
R2034 T10137 T10135 pobj mutations,to
R2035 T10138 T10137 compound disease,mutations
R2036 T10164 T10160 pobj vesicles,to
R2037 T10139 T10129 auxpass are,transfected
R2038 T10140 T10129 prep into,transfected
R2039 T10141 T10142 compound kidney,lines
R2040 T10165 T10147 punct .,fail
R2041 T10142 T10140 pobj lines,into
R2042 T10143 T10142 compound cell,lines
R2043 T10144 T10122 punct .,comes
R2044 T10167 T10168 advmod Rather,trapped
R2045 T10169 T10168 punct ", ",trapped
R2046 T10170 T10168 nsubjpass they,trapped
R2047 T10171 T10168 auxpass get,trapped
R2048 T10172 T10168 prep in,trapped
R2049 T10250 T10237 parataxis row,translocated
R2050 T10251 T10250 dep Figure,row
R2051 T10173 T10174 det the,reticulum
R2052 T10252 T10251 nummod 4A,Figure
R2053 T10253 T10250 punct ", ",row
R2054 T10174 T10172 pobj reticulum,in
R2055 T10254 T10250 amod second,row
R2056 T10255 T10250 punct ),row
R2057 T10256 T10237 punct .,translocated
R2058 T10175 T10174 amod endoplasmic,reticulum
R2059 T10258 T10259 prep In,distributed
R2060 T10176 T10174 punct (,reticulum
R2061 T10260 T10258 pobj kidneys,In
R2062 T10261 T10260 acl taken,kidneys
R2063 T10177 T10174 appos ER,reticulum
R2064 T10262 T10261 prep from,taken
R2065 T10263 T10264 compound mutant,animals
R2066 T10264 T10262 pobj animals,from
R2067 T10178 T10168 punct ),trapped
R2068 T10265 T10259 punct ", ",distributed
R2069 T10266 T10259 advmod however,distributed
R2070 T10267 T10259 punct ", ",distributed
R2071 T10179 T10168 punct .,trapped
R2072 T10268 T10259 nsubjpass AQP2,distributed
R2073 T10269 T10259 auxpass was,distributed
R2074 T10270 T10259 advmod randomly,distributed
R2075 T10271 T10259 prep throughout,distributed
R2076 T10181 T10182 poss Our,model
R2077 T10272 T10273 det the,cell
R2078 T10273 T10271 pobj cell,throughout
R2079 T10274 T10259 prep in,distributed
R2080 T10275 T10276 det the,state
R2081 T10182 T10184 nsubj model,affords
R2082 T10276 T10274 pobj state,in
R2083 T10277 T10276 amod basal,state
R2084 T10278 T10279 punct (,row
R2085 T10183 T10182 compound mouse,model
R2086 T10185 T10182 prep of,model
R2087 T10279 T10259 parataxis row,distributed
R2088 T10186 T10185 pobj NDI,of
R2089 T10280 T10279 dep Figure,row
R2090 T10281 T10280 nummod 4A,Figure
R2091 T10187 T10188 det the,opportunity
R2092 T10282 T10279 punct ", ",row
R2093 T10283 T10279 amod third,row
R2094 T10284 T10279 punct ),row
R2095 T10188 T10184 dobj opportunity,affords
R2096 T10285 T10259 punct ", ",distributed
R2097 T10286 T10287 mark while,localized
R2098 T10189 T10188 amod first,opportunity
R2099 T10287 T10259 advcl localized,distributed
R2100 T10288 T10287 nsubj AQP3,localized
R2101 T10289 T10290 punct (,green
R2102 T10290 T10288 parataxis green,AQP3
R2103 T10291 T10290 punct ),green
R2104 T10190 T10191 aux to,test
R2105 T10292 T10287 advmod appropriately,localized
R2106 T10293 T10287 prep to,localized
R2107 T10294 T10295 det the,surface
R2108 T10191 T10188 acl test,opportunity
R2109 T10295 T10293 pobj surface,to
R2110 T10296 T10295 amod basolateral,surface
R2111 T10297 T10298 punct [,23
R2112 T10192 T10193 det this,hypothesis
R2113 T10298 T10287 parataxis 23,localized
R2114 T10299 T10298 punct ],23
R2115 T10300 T10259 punct .,distributed
R2116 T10193 T10191 dobj hypothesis,test
R2117 T10302 T10303 advmod Furthermore,failed
R2118 T10194 T10191 prep in,test
R2119 T10304 T10303 punct ", ",failed
R2120 T10305 T10303 prep upon,failed
R2121 T10306 T10307 compound dDAVP,stimulation
R2122 T10195 T10196 det a,animal
R2123 T10307 T10305 pobj stimulation,upon
R2124 T10308 T10303 punct ", ",failed
R2125 T10309 T10310 compound AQP2,F204V
R2126 T10196 T10194 pobj animal,in
R2127 T10310 T10303 nsubj F204V,failed
R2128 T10311 T10310 punct -,F204V
R2129 T10312 T10313 aux to,translocate
R2130 T10197 T10196 amod mature,animal
R2131 T10313 T10303 xcomp translocate,failed
R2132 T10314 T10313 prep to,translocate
R2133 T10198 T10184 punct .,affords
R2134 T10315 T10316 det the,surface
R2135 T10316 T10314 pobj surface,to
R2136 T10317 T10316 compound cell,surface
R2137 T10200 T10201 mark As,shown
R2138 T10318 T10319 punct (,row
R2139 T10319 T10303 parataxis row,failed
R2140 T10320 T10319 dep Figure,row
R2141 T10321 T10320 nummod 4A,Figure
R2142 T10322 T10319 punct ", ",row
R2143 T10201 T10202 advcl shown,localized
R2144 T10323 T10319 amod bottom,row
R2145 T10324 T10319 punct ),row
R2146 T10203 T10201 prep in,shown
R2147 T10325 T10303 punct .,failed
R2148 T10327 T10328 aux To,confirm
R2149 T10328 T10329 advcl confirm,repeated
R2150 T10204 T10203 pobj Figure,in
R2151 T10330 T10331 det these,findings
R2152 T10331 T10328 dobj findings,confirm
R2153 T10332 T10329 punct ", ",repeated
R2154 T10333 T10334 det the,staining
R2155 T10334 T10329 nsubjpass staining,repeated
R2156 T10205 T10204 nummod 4A,Figure
R2157 T10335 T10329 auxpass was,repeated
R2158 T10336 T10329 prep in,repeated
R2159 T10337 T10336 pobj kidneys,in
R2160 T10206 T10207 punct (,row
R2161 T10338 T10337 acl taken,kidneys
R2162 T10339 T10338 prep from,taken
R2163 T10340 T10341 nummod two,mice
R2164 T10341 T10339 pobj mice,from
R2165 T10207 T10204 parataxis row,Figure
R2166 T10342 T10341 amod further,mice
R2167 T10343 T10338 prep for,taken
R2168 T10208 T10207 amod top,row
R2169 T10344 T10345 det each,class
R2170 T10345 T10343 pobj class,for
R2171 T10209 T10207 prep of,row
R2172 T10346 T10345 punct ", ",class
R2173 T10347 T10348 amod wild,type
R2174 T10348 T10345 appos type,class
R2175 T10210 T10209 pobj photomicrographs,of
R2176 T10349 T10348 punct -,type
R2177 T10350 T10348 cc or,type
R2178 T10351 T10348 conj mutant,type
R2179 T10211 T10207 punct ),row
R2180 T10352 T10329 punct ", ",repeated
R2181 T10353 T10329 prep with,repeated
R2182 T10354 T10353 cc or,with
R2183 T10212 T10202 punct ", ",localized
R2184 T10355 T10353 conj without,with
R2185 T10213 T10202 nsubj AQP2,localized
R2186 T10214 T10213 punct (,AQP2
R2187 T10215 T10213 acl stained,AQP2
R2188 T10356 T10357 compound dDAVP,treatment
R2189 T10357 T10355 pobj treatment,without
R2190 T10216 T10215 oprd red,stained
R2191 T10358 T10329 punct ", ",repeated
R2192 T10359 T10329 prep with,repeated
R2193 T10217 T10213 punct ),AQP2
R2194 T10360 T10361 amod identical,results
R2195 T10361 T10359 pobj results,with
R2196 T10362 T10329 punct .,repeated
R2197 T10218 T10202 advmod normally,localized
R2198 T10364 T10365 aux To,investigate
R2199 T10365 T10366 advcl investigate,turned
R2200 T10219 T10202 prep to,localized
R2201 T10367 T10368 det the,mechanism
R2202 T10220 T10221 det the,region
R2203 T10368 T10365 dobj mechanism,investigate
R2204 T10221 T10219 pobj region,to
R2205 T10369 T10368 prep of,mechanism
R2206 T10370 T10371 amod defective,translocation
R2207 T10222 T10221 amod subapical,region
R2208 T10371 T10369 pobj translocation,of
R2209 T10372 T10371 prep of,translocation
R2210 T10223 T10221 prep of,region
R2211 T10373 T10374 compound AQP2,F204V
R2212 T10374 T10372 pobj F204V,of
R2213 T10375 T10374 punct -,F204V
R2214 T10224 T10225 amod collecting,duct
R2215 T10376 T10366 punct ", ",turned
R2216 T10377 T10366 nsubj we,turned
R2217 T10225 T10226 compound duct,cells
R2218 T10378 T10366 prep to,turned
R2219 T10379 T10378 pobj transfection,to
R2220 T10226 T10223 pobj cells,of
R2221 T10380 T10379 prep of,transfection
R2222 T10381 T10382 compound MDCK,cells
R2223 T10382 T10380 pobj cells,of
R2224 T10227 T10221 prep in,region
R2225 T10383 T10366 punct .,turned
R2226 T10228 T10227 pobj kidneys,in
R2227 T10385 T10386 amod Stable,lines
R2228 T10386 T10388 nsubjpass lines,established
R2229 T10387 T10386 compound cell,lines
R2230 T10229 T10228 prep of,kidneys
R2231 T10389 T10386 acl expressing,lines
R2232 T10390 T10391 nmod mouse,AQP2
R2233 T10230 T10231 amod wild,type
R2234 T10391 T10389 dobj AQP2,expressing
R2235 T10392 T10393 amod wild,type
R2236 T10393 T10391 compound type,AQP2
R2237 T10231 T10233 compound type,mice
R2238 T10394 T10393 punct -,type
R2239 T10395 T10391 cc and,AQP2
R2240 T10232 T10231 punct -,type
R2241 T10396 T10397 compound AQP2,F204V
R2242 T10397 T10391 conj F204V,AQP2
R2243 T10398 T10397 punct -,F204V
R2244 T10233 T10229 pobj mice,of
R2245 T10399 T10388 auxpass were,established
R2246 T10400 T10388 punct .,established
R2247 T10234 T10202 punct .,localized
R2248 T10402 T10403 nsubj Immunoblots,showed
R2249 T10236 T10237 prep Upon,translocated
R2250 T10404 T10402 prep of,Immunoblots
R2251 T10405 T10406 compound protein,extracts
R2252 T10406 T10404 pobj extracts,of
R2253 T10407 T10406 prep from,extracts
R2254 T10408 T10409 amod stable,lines
R2255 T10409 T10407 pobj lines,from
R2256 T10410 T10409 compound cell,lines
R2257 T10238 T10236 pobj stimulation,Upon
R2258 T10411 T10412 mark that,recapitulate
R2259 T10412 T10403 ccomp recapitulate,showed
R2260 T10413 T10414 compound MDCK,cells
R2261 T10414 T10412 nsubj cells,recapitulate
R2262 T10415 T10416 det the,defect
R2263 T10239 T10238 prep with,stimulation
R2264 T10416 T10412 dobj defect,recapitulate
R2265 T10417 T10416 compound glycosylation,defect
R2266 T10240 T10239 pobj dDAVP,with
R2267 T10418 T10416 acl seen,defect
R2268 T10419 T10418 prep in,seen
R2269 T10420 T10421 compound mutant,mice
R2270 T10241 T10237 punct ", ",translocated
R2271 T10421 T10419 pobj mice,in
R2272 T10422 T10423 punct (,data
R2273 T10423 T10403 meta data,showed
R2274 T10424 T10423 amod unpublished,data
R2275 T10242 T10237 nsubj AQP2,translocated
R2276 T10425 T10423 punct ),data
R2277 T10426 T10403 punct .,showed
R2278 T10243 T10237 prep to,translocated
R2279 T10428 T10429 det The,protein
R2280 T10429 T10433 nsubj protein,was
R2281 T10244 T10243 cc or,to
R2282 T10430 T10431 amod wild,type
R2283 T10431 T10429 compound type,protein
R2284 T10432 T10431 punct -,type
R2285 T10245 T10243 conj near,to
R2286 T10434 T10433 advmod again,was
R2287 T10246 T10247 det the,surface
R2288 T10435 T10433 acomp present,was
R2289 T10436 T10433 prep in,was
R2290 T10437 T10438 nummod three,forms
R2291 T10247 T10245 pobj surface,near
R2292 T10438 T10436 pobj forms,in
R2293 T10439 T10438 amod different,forms
R2294 T10248 T10247 compound cell,surface
R2295 T10440 T10433 punct .,was
R2296 T10442 T10443 nsubj Cells,lacked
R2297 T10249 T10250 punct (,row
R2298 T10444 T10442 acl expressing,Cells
R2299 T10445 T10446 compound AQP2,F204V
R2300 T10462 T10461 punct -,glycosylated
R2301 T10446 T10444 dobj F204V,expressing
R2302 T10447 T10446 punct -,F204V
R2303 T10448 T10449 det the,form
R2304 T10449 T10443 dobj form,lacked
R2305 T10463 T10464 nummod 31,kDa
R2306 T10450 T10451 quantmod 35,45
R2307 T10451 T10453 nummod 45,kDa
R2308 T10452 T10451 punct –,45
R2309 T10453 T10449 compound kDa,form
R2310 T10454 T10443 cc and,lacked
R2311 T10455 T10456 auxpass were,enriched
R2312 T10464 T10459 compound kDa,form
R2313 T10456 T10443 conj enriched,lacked
R2314 T10457 T10456 prep in,enriched
R2315 T10465 T10443 punct .,lacked
R2316 T10458 T10459 det the,form
R2317 T10467 T10468 prep In,appeared
R2318 T10459 T10457 pobj form,in
R2319 T10460 T10461 npadvmod core,glycosylated
R2320 T10461 T10459 amod glycosylated,form
R2321 T10469 T10470 amod transfected,cells
R2322 T10470 T10467 pobj cells,In
R2323 T10471 T10470 punct ", ",cells
R2324 T10567 T10568 prep Along,seen
R2325 T10472 T10470 amod unstimulated,cells
R2326 T10569 T10570 det the,axis
R2327 T10570 T10567 pobj axis,Along
R2328 T10473 T10470 compound MDCK,cells
R2329 T10571 T10570 compound z,axis
R2330 T10572 T10570 punct -,axis
R2331 T10573 T10568 punct ", ",seen
R2332 T10474 T10468 punct ", ",appeared
R2333 T10574 T10575 det the,distribution
R2334 T10475 T10476 amod wild,type
R2335 T10476 T10478 compound type,AQP2
R2336 T10575 T10568 nsubjpass distribution,seen
R2337 T10576 T10575 amod perinuclear,distribution
R2338 T10477 T10476 punct -,type
R2339 T10577 T10575 prep of,distribution
R2340 T10578 T10579 compound AQP2,F204V
R2341 T10478 T10468 nsubj AQP2,appeared
R2342 T10579 T10577 pobj F204V,of
R2343 T10580 T10579 punct -,F204V
R2344 T10581 T10568 auxpass was,seen
R2345 T10582 T10568 advmod clearly,seen
R2346 T10479 T10478 punct (,AQP2
R2347 T10583 T10568 punct ", ",seen
R2348 T10584 T10568 cc and,seen
R2349 T10585 T10586 det this,distribution
R2350 T10480 T10478 acl stained,AQP2
R2351 T10586 T10587 nsubjpass distribution,altered
R2352 T10587 T10568 conj altered,seen
R2353 T10588 T10587 auxpass is,altered
R2354 T10481 T10480 oprd green,stained
R2355 T10589 T10587 neg not,altered
R2356 T10590 T10587 agent by,altered
R2357 T10482 T10478 punct ),AQP2
R2358 T10591 T10590 pobj forskolin,by
R2359 T10592 T10593 punct (,columns
R2360 T10593 T10587 parataxis columns,altered
R2361 T10594 T10593 dep Figure,columns
R2362 T10595 T10594 nummod 4B,Figure
R2363 T10596 T10593 punct ", ",columns
R2364 T10483 T10468 prep in,appeared
R2365 T10597 T10598 amod bottom,row
R2366 T10598 T10593 dep row,columns
R2367 T10599 T10593 punct ", ",columns
R2368 T10484 T10485 det a,pattern
R2369 T10600 T10593 nummod two,columns
R2370 T10601 T10593 amod right,columns
R2371 T10602 T10593 punct ),columns
R2372 T10485 T10483 pobj pattern,in
R2373 T10603 T10587 punct .,altered
R2374 T10486 T10485 compound punctate,pattern
R2375 T10605 T10606 det The,distribution
R2376 T10606 T10608 nsubj distribution,is
R2377 T10607 T10606 amod perinuclear,distribution
R2378 T10487 T10485 acl distributed,pattern
R2379 T10609 T10606 prep of,distribution
R2380 T10610 T10611 compound AQP2,F204V
R2381 T10488 T10487 prep throughout,distributed
R2382 T10611 T10609 pobj F204V,of
R2383 T10612 T10611 punct -,F204V
R2384 T10489 T10490 det the,region
R2385 T10613 T10608 acomp consistent,is
R2386 T10614 T10613 prep with,consistent
R2387 T10615 T10616 det an,compartmentalization
R2388 T10616 T10614 pobj compartmentalization,with
R2389 T10490 T10488 pobj region,throughout
R2390 T10617 T10616 compound ER,compartmentalization
R2391 T10618 T10608 punct .,is
R2392 T10491 T10490 amod subapical,region
R2393 T10620 T10621 aux To,test
R2394 T10621 T10622 advcl test,co-stained
R2395 T10492 T10493 punct (,photomicrographs
R2396 T10623 T10624 det the,idea
R2397 T10493 T10468 parataxis photomicrographs,appeared
R2398 T10624 T10621 dobj idea,test
R2399 T10625 T10626 mark that,localizes
R2400 T10626 T10624 acl localizes,idea
R2401 T10494 T10493 dep Figure,photomicrographs
R2402 T10627 T10628 compound AQP2,F204V
R2403 T10628 T10626 nsubj F204V,localizes
R2404 T10629 T10628 punct -,F204V
R2405 T10495 T10494 nummod 4B,Figure
R2406 T10630 T10626 prep to,localizes
R2407 T10631 T10632 det the,ER
R2408 T10496 T10493 punct ", ",photomicrographs
R2409 T10497 T10498 amod left,column
R2410 T10632 T10630 pobj ER,to
R2411 T10633 T10622 punct ", ",co-stained
R2412 T10634 T10622 nsubj we,co-stained
R2413 T10635 T10622 dobj cells,co-stained
R2414 T10636 T10635 acl transfected,cells
R2415 T10498 T10493 compound column,photomicrographs
R2416 T10637 T10636 prep with,transfected
R2417 T10638 T10637 pobj Aqp2F204V,with
R2418 T10499 T10493 punct ),photomicrographs
R2419 T10639 T10640 punct (,cDNA
R2420 T10640 T10638 parataxis cDNA,Aqp2F204V
R2421 T10500 T10468 punct ", ",appeared
R2422 T10641 T10640 punct ),cDNA
R2423 T10642 T10622 prep for,co-stained
R2424 T10643 T10642 pobj AQP2,for
R2425 T10501 T10468 advcl consistent,appeared
R2426 T10644 T10643 cc and,AQP2
R2427 T10645 T10646 det an,marker
R2428 T10646 T10643 conj marker,AQP2
R2429 T10502 T10501 prep with,consistent
R2430 T10647 T10646 compound ER,marker
R2431 T10648 T10646 punct ", ",marker
R2432 T10649 T10646 appos calnexin,marker
R2433 T10503 T10504 amod vesicular,compartmentalization
R2434 T10650 T10651 punct (,Figure
R2435 T10651 T10622 parataxis Figure,co-stained
R2436 T10652 T10651 nummod 4C,Figure
R2437 T10504 T10502 pobj compartmentalization,with
R2438 T10653 T10651 punct ),Figure
R2439 T10654 T10622 punct .,co-stained
R2440 T10505 T10468 punct .,appeared
R2441 T10656 T10657 nsubjpass Colocalization,investigated
R2442 T10658 T10656 prep of,Colocalization
R2443 T10507 T10508 compound AQP2,F204V
R2444 T10659 T10658 pobj calnexin,of
R2445 T10660 T10656 prep with,Colocalization
R2446 T10661 T10660 pobj AQP2,with
R2447 T10662 T10657 auxpass was,investigated
R2448 T10508 T10510 nsubj F204V,appeared
R2449 T10663 T10657 advmod directly,investigated
R2450 T10664 T10657 punct ", ",investigated
R2451 T10665 T10657 cc and,investigated
R2452 T10509 T10508 punct -,F204V
R2453 T10666 T10667 nsubjpass it,found
R2454 T10667 T10657 conj found,investigated
R2455 T10668 T10667 auxpass was,found
R2456 T10511 T10510 punct ", ",appeared
R2457 T10669 T10670 mark that,colocalized
R2458 T10670 T10667 ccomp colocalized,found
R2459 T10671 T10672 nummod 80,%
R2460 T10672 T10670 nsubj %,colocalized
R2461 T10512 T10510 prep on,appeared
R2462 T10513 T10514 det the,hand
R2463 T10673 T10672 prep of,%
R2464 T10514 T10512 pobj hand,on
R2465 T10674 T10675 det all,protein
R2466 T10675 T10673 pobj protein,of
R2467 T10676 T10677 compound AQP2,F204V
R2468 T10515 T10514 amod other,hand
R2469 T10677 T10675 compound F204V,protein
R2470 T10678 T10677 punct -,F204V
R2471 T10679 T10670 prep with,colocalized
R2472 T10516 T10510 punct ", ",appeared
R2473 T10680 T10679 pobj calnexin,with
R2474 T10681 T10667 punct .,found
R2475 T10517 T10510 prep in,appeared
R2476 T10683 T10684 det The,%
R2477 T10684 T10687 nsubj %,appeared
R2478 T10518 T10519 det a,pattern
R2479 T10685 T10684 amod remaining,%
R2480 T10686 T10684 nummod 20,%
R2481 T10519 T10517 pobj pattern,in
R2482 T10688 T10687 prep at,appeared
R2483 T10689 T10690 det the,periphery
R2484 T10520 T10521 npadvmod punctate,perinuclear
R2485 T10690 T10688 pobj periphery,at
R2486 T10691 T10690 prep of,periphery
R2487 T10692 T10693 det the,ER
R2488 T10521 T10519 amod perinuclear,pattern
R2489 T10693 T10691 pobj ER,of
R2490 T10694 T10687 punct ", ",appeared
R2491 T10695 T10687 advcl representing,appeared
R2492 T10522 T10521 cc but,perinuclear
R2493 T10696 T10697 compound AQP2,F204V
R2494 T10697 T10695 dobj F204V,representing
R2495 T10698 T10697 punct -,F204V
R2496 T10523 T10524 punct (,column
R2497 T10699 T10700 dep that,progressed
R2498 T10700 T10697 relcl progressed,F204V
R2499 T10524 T10510 parataxis column,appeared
R2500 T10701 T10700 aux had,progressed
R2501 T10702 T10700 advmod potentially,progressed
R2502 T10703 T10700 prep beyond,progressed
R2503 T10525 T10524 dep Figure,column
R2504 T10704 T10705 det the,ER
R2505 T10705 T10703 pobj ER,beyond
R2506 T10706 T10687 punct .,appeared
R2507 T10526 T10525 nummod 4B,Figure
R2508 T10708 T10709 det This,escape
R2509 T10709 T10712 nsubj escape,was
R2510 T10527 T10524 punct ", ",column
R2511 T10710 T10709 punct “,escape
R2512 T10711 T10709 compound ER,escape
R2513 T10528 T10524 amod third,column
R2514 T10713 T10709 punct ”,escape
R2515 T10714 T10712 acomp consistent,was
R2516 T10715 T10714 prep with,consistent
R2517 T10716 T10717 det the,proportion
R2518 T10717 T10715 pobj proportion,with
R2519 T10529 T10524 punct ),column
R2520 T10718 T10717 amod small,proportion
R2521 T10530 T10510 punct .,appeared
R2522 T10719 T10717 prep of,proportion
R2523 T10720 T10721 amod mature,F204V
R2524 T10721 T10719 pobj F204V,of
R2525 T10532 T10533 prep Upon,translocated
R2526 T10722 T10721 punct ", ",F204V
R2527 T10723 T10724 amod complex,glycosylated
R2528 T10724 T10721 amod glycosylated,F204V
R2529 T10725 T10721 punct ", ",F204V
R2530 T10534 T10532 pobj stimulation,Upon
R2531 T10726 T10721 compound AQP2,F204V
R2532 T10727 T10721 punct -,F204V
R2533 T10728 T10717 prep in,proportion
R2534 T10535 T10534 prep with,stimulation
R2535 T10729 T10730 compound mutant,kidneys
R2536 T10730 T10728 pobj kidneys,in
R2537 T10731 T10732 punct (,see
R2538 T10536 T10535 pobj forskolin,with
R2539 T10732 T10712 parataxis see,was
R254 T2464 T2465 amod Nephrogenic,insipidus
R2540 T10733 T10732 dobj Figure,see
R2541 T10537 T10533 punct ", ",translocated
R2542 T10734 T10733 nummod 3A,Figure
R2543 T10735 T10732 punct ),see
R2544 T10736 T10712 punct .,was
R2545 T10538 T10539 det a,activator
R2546 T10738 T10739 nsubjpass Animals,affected
R2547 T10539 T10533 nsubj activator,translocated
R2548 T10740 T10738 amod heterozygous,Animals
R2549 T10741 T10740 prep for,heterozygous
R255 T2465 T2467 nsubj insipidus,is
R2550 T10742 T10743 det the,mutation
R2551 T10540 T10541 npadvmod cAMP,dependent
R2552 T10743 T10741 pobj mutation,for
R2553 T10744 T10743 compound Aqp2F204V,mutation
R2554 T10745 T10739 auxpass were,affected
R2555 T10541 T10539 amod dependent,activator
R2556 T10746 T10739 neg not,affected
R2557 T10747 T10739 prep in,affected
R2558 T10748 T10749 poss their,production
R2559 T10542 T10541 punct -,dependent
R256 T2466 T2465 compound diabetes,insipidus
R2560 T10749 T10747 pobj production,in
R2561 T10750 T10749 compound urine,production
R2562 T10751 T10749 cc or,production
R2563 T10543 T10544 compound protein,kinase
R2564 T10752 T10753 compound urine,osmolality
R2565 T10753 T10749 conj osmolality,production
R2566 T10754 T10755 punct (,see
R2567 T10755 T10739 parataxis see,affected
R2568 T10756 T10757 nmod Figure,1C
R2569 T10757 T10755 dobj 1C,see
R257 T2468 T2465 punct (,insipidus
R2570 T10544 T10539 compound kinase,activator
R2571 T10758 T10757 cc and,1C
R2572 T10759 T10757 conj 1D,1C
R2573 T10760 T10755 punct ),see
R2574 T10545 T10539 punct ", ",activator
R2575 T10761 T10739 punct .,affected
R2576 T10763 T10764 nsubjpass It,shown
R2577 T10546 T10547 amod wild,type
R2578 T10765 T10764 aux has,shown
R2579 T10766 T10764 advmod also,shown
R258 T2469 T2465 appos NDI,insipidus
R2580 T10547 T10549 compound type,AQP2
R2581 T10767 T10764 auxpass been,shown
R2582 T10768 T10769 mark that,interact
R2583 T10769 T10764 ccomp interact,shown
R2584 T10548 T10547 punct -,type
R2585 T10770 T10771 det a,allele
R2586 T10771 T10769 nsubj allele,interact
R2587 T10772 T10771 amod recessive,allele
R2588 T10549 T10539 appos AQP2,activator
R2589 T10773 T10771 compound NDI,allele
R259 T2470 T2467 punct ),is
R2590 T10774 T10771 punct ", ",allele
R2591 T10775 T10776 compound AQP2,R187C
R2592 T10550 T10533 prep to,translocated
R2593 T10776 T10771 appos R187C,allele
R2594 T10777 T10776 punct -,R187C
R2595 T10778 T10769 punct ", ",interact
R2596 T10551 T10552 det the,surface
R2597 T10552 T10550 pobj surface,to
R2598 T10553 T10552 amod apical,surface
R2599 T10779 T10769 aux does,interact
R260 T2471 T2472 det a,disease
R2600 T10554 T10552 prep of,surface
R2601 T10780 T10769 neg not,interact
R2602 T10781 T10769 prep with,interact
R2603 T10782 T10783 amod wild,type
R2604 T10555 T10556 amod polarized,cells
R2605 T10783 T10785 compound type,protein
R2606 T10784 T10783 punct -,type
R2607 T10556 T10554 pobj cells,of
R2608 T10785 T10781 pobj protein,with
R2609 T10786 T10769 prep in,interact
R261 T2472 T2467 attr disease,is
R2610 T10787 T10786 pobj oocytes,in
R2611 T10557 T10556 compound MDCK,cells
R2612 T10788 T10789 punct [,24
R2613 T10789 T10769 parataxis 24,interact
R2614 T10790 T10789 punct ],24
R2615 T10558 T10559 punct (,column
R2616 T10791 T10769 punct ", ",interact
R2617 T10792 T10769 cc nor,interact
R2618 T10793 T10794 aux does,homo-oligomerize
R2619 T10794 T10769 conj homo-oligomerize,interact
R262 T2473 T2472 acl characterized,disease
R2620 T10795 T10794 nsubj it,homo-oligomerize
R2621 T10796 T10794 prep in,homo-oligomerize
R2622 T10559 T10533 parataxis column,translocated
R2623 T10797 T10798 compound MDCK,cells
R2624 T10798 T10796 pobj cells,in
R2625 T10799 T10800 punct [,22
R2626 T10560 T10559 dep Figure,column
R2627 T10800 T10764 parataxis 22,shown
R2628 T10801 T10800 punct ],22
R2629 T10802 T10764 punct .,shown
R263 T2474 T2473 agent by,characterized
R2630 T10561 T10560 nummod 4B,Figure
R2631 T10804 T10805 advmod Therefore,examined
R2632 T10562 T10559 punct ", ",column
R2633 T10563 T10559 amod second,column
R2634 T10806 T10805 punct ", ",examined
R2635 T10564 T10559 punct ),column
R2636 T10807 T10805 nsubjpass kidneys,examined
R2637 T10808 T10807 prep from,kidneys
R2638 T10565 T10533 punct .,translocated
R2639 T10809 T10810 amod heterozygous,animals
R264 T2475 T2476 amod excessive,urination
R2640 T10810 T10808 pobj animals,from
R2641 T10811 T10805 auxpass were,examined
R2642 T10885 T10884 prep by,undetectable
R2643 T10812 T10805 prep for,examined
R2644 T10813 T10812 pobj evidence,for
R2645 T10814 T10813 prep of,evidence
R2646 T10815 T10816 nummod two,populations
R2647 T10886 T10885 pobj immunocytochemistry,by
R2648 T10816 T10814 pobj populations,of
R2649 T10817 T10816 prep of,populations
R265 T2476 T2474 pobj urination,by
R2650 T10818 T10819 compound AQP2,protein
R2651 T10887 T10868 punct .,reflect
R2652 T10819 T10817 pobj protein,of
R2653 T10820 T10805 punct .,examined
R2654 T10889 T10890 advmod Alternatively,alter
R2655 T10822 T10823 advmod Surprisingly,revealed
R2656 T10824 T10823 punct ", ",revealed
R2657 T10891 T10890 punct ", ",alter
R2658 T10825 T10826 amod immunohistochemical,staining
R2659 T10826 T10823 nsubj staining,revealed
R266 T2477 T2476 cc and,urination
R2660 T10827 T10826 prep of,staining
R2661 T10828 T10829 nmod kidney,ducts
R2662 T10892 T10893 det the,presence
R2663 T10829 T10827 pobj ducts,of
R2664 T10830 T10829 amod collecting,ducts
R2665 T10831 T10829 prep from,ducts
R2666 T10893 T10890 nsubj presence,alter
R2667 T10832 T10833 nmod Aqp2F204V,mice
R2668 T10833 T10831 pobj mice,from
R2669 T10834 T10832 punct /,Aqp2F204V
R267 T2478 T2476 conj thirst,urination
R2670 T10835 T10832 punct +,Aqp2F204V
R2671 T10836 T10837 det a,pattern
R2672 T10837 T10823 dobj pattern,revealed
R2673 T10894 T10893 prep of,presence
R2674 T10838 T10839 advmod remarkably,similar
R2675 T10839 T10837 amod similar,pattern
R2676 T10840 T10839 prep to,similar
R2677 T10895 T10896 amod wild,type
R2678 T10841 T10842 amod wild,type
R2679 T10842 T10840 pobj type,to
R268 T2479 T2473 punct ", ",characterized
R2680 T10843 T10844 punct (,Figure
R2681 T10844 T10823 parataxis Figure,revealed
R2682 T10845 T10844 nummod 5A,Figure
R2683 T10846 T10844 punct ),Figure
R2684 T10847 T10823 punct .,revealed
R2685 T10896 T10898 compound type,protein
R2686 T10849 T10850 nsubj AQP2,translocated
R2687 T10897 T10896 punct -,type
R2688 T10851 T10850 advmod completely,translocated
R2689 T10852 T10850 prep to,translocated
R269 T2480 T2473 prep despite,characterized
R2690 T10898 T10894 pobj protein,of
R2691 T10853 T10854 det the,surface
R2692 T10854 T10852 pobj surface,to
R2693 T10855 T10854 amod apical,surface
R2694 T10856 T10854 compound cell,surface
R2695 T10899 T10890 aux may,alter
R2696 T10857 T10850 prep upon,translocated
R2697 T10858 T10859 compound dDAVP,stimulation
R2698 T10859 T10857 pobj stimulation,upon
R2699 T10900 T10901 det the,localization
R270 T2481 T2482 amod normal,production
R2700 T10860 T10850 punct .,translocated
R2701 T10901 T10890 dobj localization,alter
R2702 T10862 T10863 det This,pattern
R2703 T10863 T10868 nsubj pattern,reflect
R2704 T10864 T10865 amod wild,type
R2705 T10902 T10901 prep of,localization
R2706 T10865 T10863 compound type,pattern
R2707 T10866 T10865 punct -,type
R2708 T10867 T10863 compound staining,pattern
R2709 T10903 T10904 det the,protein
R271 T2482 T2480 pobj production,despite
R2710 T10869 T10868 aux may,reflect
R2711 T10904 T10902 pobj protein,of
R2712 T10870 T10868 advmod simply,reflect
R2713 T10871 T10872 det the,fact
R2714 T10872 T10868 dobj fact,reflect
R2715 T10873 T10874 mark that,makes
R2716 T10905 T10904 compound mutant,protein
R2717 T10874 T10872 acl makes,fact
R2718 T10875 T10874 csubj decreasing,makes
R2719 T10876 T10877 det the,amount
R272 T2483 T2482 prep of,production
R2720 T10906 T10890 punct .,alter
R2721 T10877 T10875 dobj amount,decreasing
R2722 T10878 T10877 prep of,amount
R2723 T10879 T10880 compound mutant,protein
R2724 T10880 T10878 pobj protein,of
R2725 T10881 T10875 prep by,decreasing
R2726 T10882 T10881 pobj half,by
R2727 T10908 T10909 advmod Indeed,proposed
R2728 T10883 T10884 nsubj it,undetectable
R2729 T10884 T10874 ccomp undetectable,makes
R273 T2484 T2485 det the,hormone
R2730 T10910 T10909 punct ", ",proposed
R2731 T10911 T10909 nsubj Hendriks,proposed
R2732 T10912 T10913 advmod et,al.
R2733 T10913 T10911 advmod al.,Hendriks
R2734 T10991 T10980 nsubj type,rescue
R2735 T10914 T10915 det a,mechanism
R2736 T10992 T10991 amod wild,type
R2737 T10915 T10909 dobj mechanism,proposed
R2738 T10993 T10980 aux may,rescue
R2739 T10994 T10995 det the,protein
R274 T2485 T2483 pobj hormone,of
R2740 T10916 T10915 punct “,mechanism
R2741 T10995 T10980 dobj protein,rescue
R2742 T10996 T10995 compound mutant,protein
R2743 T10997 T10998 mark as,suggested
R2744 T10917 T10918 nmod piggy,back
R2745 T10998 T10980 advcl suggested,rescue
R2746 T10999 T10998 agent by,suggested
R2747 T10918 T10915 nmod back,mechanism
R2748 T11000 T11001 det the,distribution
R2749 T11001 T10999 pobj distribution,by
R275 T2486 T2485 amod antidiuretic,hormone
R2750 T11002 T11001 amod subcellular,distribution
R2751 T11003 T11001 prep of,distribution
R2752 T10919 T10918 punct -,back
R2753 T11004 T11005 compound AQP2,protein
R2754 T11005 T11003 pobj protein,of
R2755 T10920 T10915 punct ”,mechanism
R2756 T11006 T10980 punct .,rescue
R2757 T11008 T11009 aux To,test
R2758 T10921 T10922 aux to,explain
R2759 T11009 T11010 advcl test,looked
R276 T2487 T2488 compound arginine,vasopressin
R2760 T10922 T10909 advcl explain,proposed
R2761 T11011 T11012 det this,idea
R2762 T11012 T11009 dobj idea,test
R2763 T11013 T11010 punct ", ",looked
R2764 T11014 T11010 nsubj we,looked
R2765 T10923 T10924 det the,transport
R2766 T11015 T11010 advmod first,looked
R2767 T11016 T11010 prep for,looked
R2768 T10924 T10922 dobj transport,explain
R2769 T11017 T11018 det an,interaction
R277 T2488 T2485 appos vasopressin,hormone
R2770 T11018 T11016 pobj interaction,for
R2771 T11019 T11018 prep between,interaction
R2772 T10925 T10924 prep of,transport
R2773 T11020 T11021 nmod mutant,proteins
R2774 T11021 T11019 pobj proteins,between
R2775 T11022 T11020 cc and,mutant
R2776 T11023 T11024 amod wild,type
R2777 T11024 T11020 conj type,mutant
R2778 T10926 T10927 amod nonglycosylated,subunits
R2779 T11025 T11024 punct -,type
R278 T2489 T2488 punct (,vasopressin
R2780 T11026 T11010 prep in,looked
R2781 T11027 T11028 amod transfected,cells
R2782 T10927 T10925 pobj subunits,of
R2783 T11028 T11026 pobj cells,in
R2784 T11029 T11028 compound MDCK,cells
R2785 T11030 T11031 punct (,Figure
R2786 T10928 T10927 prep of,subunits
R2787 T11031 T11010 parataxis Figure,looked
R2788 T11032 T11031 nummod 5B,Figure
R2789 T11033 T11031 punct ),Figure
R279 T2490 T2488 appos AVP,vasopressin
R2790 T11034 T11010 punct .,looked
R2791 T10929 T10928 pobj AQP2,of
R2792 T11036 T11037 compound MDCK,cells
R2793 T10930 T10924 prep to,transport
R2794 T11037 T11038 nsubjpass cells,transfected
R2795 T11039 T11040 advmod stably,expressing
R2796 T10931 T10932 det the,surface
R2797 T11040 T11037 acl expressing,cells
R2798 T11041 T11042 amod wild,type
R2799 T10932 T10930 pobj surface,to
R280 T2491 T2467 punct ),is
R2800 T11042 T11044 compound type,AQP2
R2801 T11043 T11042 punct -,type
R2802 T11044 T11040 dobj AQP2,expressing
R2803 T10933 T10932 compound cell,surface
R2804 T11045 T11038 auxpass were,transfected
R2805 T11046 T11038 advmod transiently,transfected
R2806 T11047 T11038 prep with,transfected
R2807 T10934 T10924 prep by,transport
R2808 T11048 T11049 compound GFP,constructs
R2809 T11049 T11047 pobj constructs,with
R281 T2492 T2493 punct [,1
R2810 T11050 T11049 compound expression,constructs
R2811 T10935 T10936 amod glycosylated,subunits
R2812 T11051 T11049 acl encoding,constructs
R2813 T11052 T11053 npadvmod GFP,tagged
R2814 T10936 T10934 pobj subunits,by
R2815 T11053 T11055 amod tagged,AQP2
R2816 T11054 T11053 punct -,tagged
R2817 T11055 T11051 dobj AQP2,encoding
R2818 T10937 T10938 punct [,22
R2819 T11056 T11057 amod wild,type
R282 T2493 T2467 parataxis 1,is
R2820 T11057 T11055 compound type,AQP2
R2821 T11058 T11057 punct -,type
R2822 T10938 T10922 parataxis 22,explain
R2823 T11059 T11055 punct ", ",AQP2
R2824 T11060 T11061 compound AQP2,F204V
R2825 T11061 T11055 conj F204V,AQP2
R2826 T11062 T11061 punct -,F204V
R2827 T11063 T11061 punct ", ",F204V
R2828 T11064 T11061 cc or,F204V
R2829 T10939 T10938 punct ],22
R283 T2494 T2493 punct ],1
R2830 T11065 T11061 conj GFP,F204V
R2831 T11066 T11065 advmod alone,GFP
R2832 T10940 T10909 punct .,proposed
R2833 T10942 T10943 nsubjpass It,shown
R2834 T11067 T11038 punct .,transfected
R2835 T10944 T10943 aux has,shown
R2836 T11069 T11070 nsubj Antibodies,coimmunoprecipitated
R2837 T11071 T11069 prep against,Antibodies
R2838 T11072 T11071 pobj GFP,against
R2839 T10945 T10943 advmod also,shown
R284 T2495 T2467 punct .,is
R2840 T11073 T11074 amod wild,type
R2841 T11074 T11076 compound type,AQP2
R2842 T10946 T10943 auxpass been,shown
R2843 T11075 T11074 punct -,type
R2844 T11076 T11070 dobj AQP2,coimmunoprecipitated
R2845 T11077 T11078 advmod when,transfected
R2846 T10947 T10948 mark that,rescue
R2847 T11078 T11070 advcl transfected,coimmunoprecipitated
R2848 T11079 T11080 compound AQP2,GFP
R2849 T10948 T10943 ccomp rescue,shown
R285 T2497 T2498 det The,forms
R2850 T11080 T11078 nsubjpass GFP,transfected
R2851 T11081 T11080 punct -,GFP
R2852 T11082 T11080 cc or,GFP
R2853 T10949 T10950 amod wild,type
R2854 T11083 T11084 compound AQP2,GFP
R2855 T11084 T11080 conj GFP,GFP
R2856 T11085 T11084 punct -,GFP
R2857 T10950 T10952 compound type,protein
R2858 T11086 T11084 compound F204V,GFP
R2859 T11087 T11084 punct -,GFP
R286 T2498 T2500 nsubj forms,are
R2860 T10951 T10950 punct -,type
R2861 T11088 T11078 auxpass was,transfected
R2862 T11089 T11078 advmod transiently,transfected
R2863 T11090 T11078 punct ", ",transfected
R2864 T10952 T10948 nsubj protein,rescue
R2865 T11091 T11078 cc but,transfected
R2866 T11092 T11091 neg not,but
R2867 T11093 T11094 advmod when,transfected
R2868 T10953 T10952 compound AQP2,protein
R2869 T11094 T11078 conj transfected,transfected
R287 T2499 T2498 amod inherited,forms
R2870 T11095 T11094 nsubjpass GFP,transfected
R2871 T10954 T10948 aux can,rescue
R2872 T11096 T11095 prep by,GFP
R2873 T10955 T10956 det a,protein
R2874 T10956 T10948 dobj protein,rescue
R2875 T10957 T10958 npadvmod translocation,defective
R2876 T11097 T11096 pobj itself,by
R2877 T11098 T11094 auxpass was,transfected
R2878 T11099 T11094 advmod transiently,transfected
R2879 T11100 T11094 prep into,transfected
R288 T2501 T2502 advmod either,linked
R2880 T11101 T11102 compound MDCK,cells
R2881 T10958 T10956 amod defective,protein
R2882 T11102 T11100 pobj cells,into
R2883 T11103 T11104 advmod stably,expressing
R2884 T11104 T11102 acl expressing,cells
R2885 T10959 T10958 punct -,defective
R2886 T11105 T11106 amod wild,type
R2887 T11106 T11108 compound type,AQP2
R2888 T11107 T11106 punct -,type
R2889 T10960 T10956 compound mutant,protein
R289 T2502 T2500 acomp linked,are
R2890 T11108 T11104 dobj AQP2,expressing
R2891 T11109 T11110 punct (,blots
R2892 T10961 T10956 punct ", ",protein
R2893 T11110 T11070 parataxis blots,coimmunoprecipitated
R2894 T11111 T11110 dep Figure,blots
R2895 T11112 T11111 nummod 5B,Figure
R2896 T10962 T10963 compound AQP2,P262L
R2897 T11113 T11110 punct ", ",blots
R2898 T11114 T11110 amod upper,blots
R2899 T11115 T11110 punct ),blots
R290 T2503 T2502 npadvmod X,linked
R2900 T10963 T10956 appos P262L,protein
R2901 T11116 T11070 punct .,coimmunoprecipitated
R2902 T10964 T10963 punct -,P262L
R2903 T11118 T11119 compound Western,blot
R2904 T11119 T11120 nsubj blot,showed
R2905 T10965 T10948 punct ", ",rescue
R2906 T11121 T11119 prep of,blot
R2907 T11122 T11123 amod total,membranes
R2908 T11123 T11121 pobj membranes,of
R2909 T10966 T10967 advmod when,coexpressed
R291 T2504 T2502 punct -,linked
R2910 T11124 T11125 mark that,expressed
R2911 T11125 T11120 ccomp expressed,showed
R2912 T11126 T11127 amod wild,type
R2913 T10967 T10948 advcl coexpressed,rescue
R2914 T11127 T11129 compound type,AQP2
R2915 T11128 T11127 punct -,type
R2916 T11129 T11125 nsubjpass AQP2,expressed
R2917 T10968 T10969 det the,two
R2918 T11130 T11125 auxpass is,expressed
R2919 T11131 T11125 advmod equivalently,expressed
R292 T2505 T2502 prep as,linked
R2920 T10969 T10967 nsubjpass two,coexpressed
R2921 T11132 T11125 prep in,expressed
R2922 T11133 T11134 det all,cases
R2923 T11134 T11132 pobj cases,in
R2924 T11135 T11134 nummod three,cases
R2925 T10970 T10967 auxpass are,coexpressed
R2926 T11136 T11137 punct (,blots
R2927 T11137 T11120 parataxis blots,showed
R2928 T11138 T11137 dep Figure,blots
R2929 T11139 T11138 nummod 5B,Figure
R293 T2506 T2507 det a,consequence
R2930 T11140 T11137 punct ", ",blots
R2931 T11141 T11137 amod lower,blots
R2932 T10971 T10967 prep in,coexpressed
R2933 T11142 T11137 punct ),blots
R2934 T11143 T11120 punct .,showed
R2935 T11145 T11146 mark If,interacting
R2936 T10972 T10973 compound MDCK,cells
R2937 T10973 T10971 pobj cells,in
R2938 T11146 T11155 advcl interacting,is
R2939 T10974 T10975 punct [,25
R294 T2507 T2505 pobj consequence,as
R2940 T11147 T11148 amod wild,type
R2941 T11148 T11150 nmod type,proteins
R2942 T11149 T11148 punct -,type
R2943 T11150 T11146 nsubj proteins,interacting
R2944 T10975 T10943 parataxis 25,shown
R2945 T11151 T11148 cc and,type
R2946 T11152 T11148 conj mutant,type
R2947 T11153 T11146 aux are,interacting
R2948 T10976 T10975 punct ],25
R2949 T11154 T11146 advmod indeed,interacting
R295 T2508 T2507 prep of,consequence
R2950 T11156 T11146 prep in,interacting
R2951 T10977 T10943 punct .,shown
R2952 T11157 T11158 det the,cell
R2953 T11158 T11156 pobj cell,in
R2954 T11159 T11155 punct ", ",is
R2955 T10979 T10980 prep In,rescue
R2956 T11160 T11161 det this,interaction
R2957 T11161 T11155 nsubj interaction,is
R2958 T11162 T11155 acomp sufficient,is
R2959 T11163 T11164 aux to,rescue
R296 T2509 T2508 pobj mutation,of
R2960 T10981 T10982 det the,ducts
R2961 T11164 T11162 xcomp rescue,sufficient
R2962 T11165 T11166 det the,localization
R2963 T11166 T11164 dobj localization,rescue
R2964 T10982 T10979 pobj ducts,In
R2965 T11167 T11166 prep of,localization
R2966 T11168 T11169 compound mutant,protein
R2967 T11169 T11167 pobj protein,of
R2968 T11170 T11155 punct ?,is
R2969 T10983 T10982 amod collecting,ducts
R297 T2510 T2509 prep of,mutation
R2970 T11172 T11173 aux To,answer
R2971 T11173 T11174 advcl answer,used
R2972 T10984 T10982 prep from,ducts
R2973 T11175 T11176 det this,question
R2974 T11176 T11173 dobj question,answer
R2975 T10985 T10986 nmod Aqp2F204V,mice
R2976 T11177 T11174 punct ", ",used
R2977 T11178 T11174 nsubj we,used
R2978 T10986 T10984 pobj mice,from
R2979 T11179 T11180 compound MDCK,cells
R298 T2511 T2512 det the,gene
R2980 T11180 T11174 dobj cells,used
R2981 T11181 T11182 advmod stably,transfected
R2982 T11182 T11180 acl transfected,cells
R2983 T11183 T11182 prep with,transfected
R2984 T11184 T11185 amod wild,type
R2985 T10987 T10985 punct /,Aqp2F204V
R2986 T11185 T11187 compound type,AQP2
R2987 T11186 T11185 punct -,type
R2988 T11187 T11183 pobj AQP2,with
R2989 T10988 T10985 punct +,Aqp2F204V
R299 T2512 T2510 pobj gene,of
R2990 T11188 T11187 acl expressing,AQP2
R2991 T11189 T11188 dobj vector,expressing
R2992 T11190 T11188 cc or,expressing
R2993 T10989 T10980 punct ", ",rescue
R2994 T11191 T11188 conj with,expressing
R2995 T11192 T11193 amod empty,vector
R2996 T11193 T11191 pobj vector,with
R2997 T10990 T10991 det the,type
R2998 T11194 T11174 punct .,used
R2999 T11196 T11197 prep On,transfected
R300 T2513 T2512 compound Avpr2,gene
R3000 T11203 T11197 advmod transiently,transfected
R3001 T11198 T11196 pobj top,On
R3002 T11199 T11198 prep of,top
R3003 T11200 T11199 pobj these,of
R3004 T11201 T11197 punct ", ",transfected
R3005 T11204 T11205 nmod AQP2,GFP
R3006 T11202 T11197 nsubj we,transfected
R3007 T11205 T11207 nmod GFP,constructs
R3008 T11206 T11205 punct -,GFP
R3009 T11207 T11197 dobj constructs,transfected
R301 T2514 T2515 punct [,2
R3010 T11208 T11205 cc or,GFP
R3011 T11308 T11306 compound F204V,GFP
R3012 T11309 T11306 punct -,GFP
R3013 T11209 T11210 compound AQP2,GFP
R3014 T11310 T11295 prep to,localized
R3015 T11311 T11312 det the,surface
R3016 T11312 T11310 pobj surface,to
R3017 T11210 T11205 conj GFP,GFP
R3018 T11313 T11312 amod apical,surface
R3019 T11314 T11295 prep to,localized
R302 T2515 T2502 parataxis 2,linked
R3020 T11211 T11210 punct -,GFP
R3021 T11315 T11316 amod varying,degrees
R3022 T11316 T11314 pobj degrees,to
R3023 T11317 T11318 punct (,images
R3024 T11212 T11210 compound F204V,GFP
R3025 T11318 T11295 parataxis images,localized
R3026 T11319 T11318 dep Figure,images
R3027 T11320 T11319 nummod 5C,Figure
R3028 T11213 T11210 punct -,GFP
R3029 T11321 T11318 punct ", ",images
R303 T2516 T2515 punct ],2
R3030 T11322 T11318 amod lower,images
R3031 T11214 T11207 compound expression,constructs
R3032 T11323 T11318 amod right,images
R3033 T11324 T11325 punct [,i
R3034 T11325 T11318 parataxis i,images
R3035 T11215 T11197 punct .,transfected
R3036 T11326 T11327 punct –,iii
R3037 T11327 T11325 prep iii,i
R3038 T11217 T11218 compound AQP2,GFP
R3039 T11328 T11325 punct ],i
R304 T2517 T2502 punct ", ",linked
R3040 T11329 T11318 punct ),images
R3041 T11330 T11295 punct .,localized
R3042 T11218 T11220 nsubj GFP,localized
R3043 T11332 T11333 det The,images
R3044 T11333 T11336 nsubj images,shows
R3045 T11219 T11218 punct -,GFP
R3046 T11334 T11335 amod lower,right
R3047 T11335 T11333 amod right,images
R3048 T11221 T11220 prep to,localized
R3049 T11337 T11333 prep of,images
R305 T2518 T2502 cc or,linked
R3050 T11338 T11337 pobj Figure,of
R3051 T11339 T11338 nummod 5C,Figure
R3052 T11222 T11223 det the,surface
R3053 T11340 T11341 nummod three,cells
R3054 T11341 T11336 dobj cells,shows
R3055 T11342 T11341 prep from,cells
R3056 T11223 T11221 pobj surface,to
R3057 T11343 T11344 det a,transfection
R3058 T11344 T11342 pobj transfection,from
R3059 T11345 T11344 amod single,transfection
R306 T2519 T2502 conj autosomal,linked
R3060 T11224 T11223 amod apical,surface
R3061 T11346 T11336 punct .,shows
R3062 T11348 T11349 det The,first
R3063 T11225 T11220 prep upon,localized
R3064 T11349 T11350 nsubj first,is
R3065 T11226 T11227 compound forskolin,stimulation
R3066 T11351 T11352 det a,cell
R3067 T11352 T11350 attr cell,is
R3068 T11353 T11352 amod nontransfected,cell
R3069 T11227 T11225 pobj stimulation,upon
R307 T2520 T2521 amod due,to
R3070 T11354 T11355 dep that,shows
R3071 T11355 T11352 relcl shows,cell
R3072 T11356 T11357 det the,localization
R3073 T11228 T11229 mark whether,transfected
R3074 T11357 T11355 dobj localization,shows
R3075 T11358 T11357 prep of,localization
R3076 T11359 T11360 det the,AQP2
R3077 T11229 T11220 advcl transfected,localized
R3078 T11360 T11358 pobj AQP2,of
R3079 T11230 T11229 nsubjpass it,transfected
R308 T2521 T2519 prep to,autosomal
R3080 T11361 T11362 advmod stably,expressing
R3081 T11231 T11229 auxpass was,transfected
R3082 T11362 T11360 amod expressing,AQP2
R3083 T11363 T11364 amod wild,type
R3084 T11232 T11229 advmod transiently,transfected
R3085 T11364 T11360 compound type,AQP2
R3086 T11365 T11364 punct -,type
R3087 T11366 T11360 punct ", ",AQP2
R3088 T11367 T11368 dep which,is
R3089 T11368 T11360 relcl is,AQP2
R309 T2522 T2521 pobj mutation,to
R3090 T11233 T11229 prep into,transfected
R3091 T11369 T11368 acomp apical,is
R3092 T11370 T11368 prep upon,is
R3093 T11371 T11372 compound forskolin,stimulation
R3094 T11234 T11235 npadvmod vector,only
R3095 T11372 T11370 pobj stimulation,upon
R3096 T11373 T11350 punct .,is
R3097 T11235 T11237 amod only,cells
R3098 T11375 T11376 det The,two
R3099 T11376 T11378 nsubj two,show
R310 T2523 T2522 prep of,mutation
R3100 T11377 T11376 amod next,two
R3101 T11236 T11235 punct -,only
R3102 T11379 T11378 dobj expression,show
R3103 T11380 T11379 prep of,expression
R3104 T11237 T11233 pobj cells,into
R3105 T11381 T11382 preconj both,AQP2
R3106 T11382 T11380 pobj AQP2,of
R3107 T11238 T11239 punct (,images
R3108 T11383 T11382 det the,AQP2
R3109 T11384 T11382 amod stable,AQP2
R311 T2524 T2525 det the,gene
R3110 T11385 T11386 amod wild,type
R3111 T11239 T11237 parataxis images,cells
R3112 T11386 T11382 compound type,AQP2
R3113 T11387 T11386 punct -,type
R3114 T11388 T11382 cc and,AQP2
R3115 T11240 T11239 dep Figure,images
R3116 T11389 T11390 det the,GFP
R3117 T11390 T11382 conj GFP,AQP2
R3118 T11391 T11390 amod transient,GFP
R3119 T11241 T11240 nummod 5C,Figure
R312 T2525 T2523 pobj gene,of
R3120 T11392 T11390 compound AQP2,GFP
R3121 T11393 T11390 punct -,GFP
R3122 T11394 T11390 compound F204V,GFP
R3123 T11242 T11239 punct ", ",images
R3124 T11395 T11390 punct -,GFP
R3125 T11396 T11378 punct .,show
R3126 T11243 T11239 amod upper,images
R3127 T11398 T11399 prep In,was
R3128 T11399 T11411 ccomp was,were
R3129 T11244 T11239 amod left,images
R313 T2526 T2525 compound Aqp2,gene
R3130 T11400 T11398 pobj cell,In
R3131 T11401 T11400 punct (,cell
R3132 T11402 T11400 nummod ii,cell
R3133 T11403 T11400 punct ),cell
R3134 T11245 T11239 punct ),images
R3135 T11404 T11399 punct ", ",was
R3136 T11405 T11399 nsubj localization,was
R3137 T11406 T11405 prep of,localization
R3138 T11407 T11408 amod wild,type
R3139 T11408 T11410 compound type,AQP2
R314 T2527 T2528 punct [,3
R3140 T11409 T11408 punct -,type
R3141 T11246 T11233 cc or,into
R3142 T11410 T11406 pobj AQP2,of
R3143 T11412 T11399 acomp indistinguishable,was
R3144 T11247 T11233 conj into,into
R3145 T11248 T11249 amod wild,type
R3146 T11249 T11251 compound type,cells
R3147 T11413 T11412 prep from,indistinguishable
R3148 T11414 T11415 compound AQP2,GFP
R3149 T11250 T11249 punct -,type
R315 T2528 T2519 parataxis 3,autosomal
R3150 T11251 T11247 pobj cells,into
R3151 T11415 T11413 pobj GFP,from
R3152 T11416 T11415 punct -,GFP
R3153 T11252 T11251 compound AQP2,cells
R3154 T11417 T11415 compound F204V,GFP
R3155 T11418 T11415 punct -,GFP
R3156 T11419 T11411 punct ;,were
R3157 T11253 T11254 punct (,Figure
R3158 T11420 T11411 nsubj both,were
R3159 T11421 T11411 acomp apical,were
R316 T2529 T2528 punct ],3
R3160 T11254 T11251 parataxis Figure,cells
R3161 T11422 T11411 prep upon,were
R3162 T11423 T11424 compound forskolin,stimulation
R3163 T11255 T11254 nummod 5C,Figure
R3164 T11424 T11422 pobj stimulation,upon
R3165 T11425 T11411 punct .,were
R3166 T11256 T11254 punct ", ",Figure
R3167 T11427 T11428 mark Although,was
R3168 T11428 T11431 advcl was,localized
R3169 T11257 T11258 amod upper,right
R317 T2530 T2500 punct .,are
R3170 T11429 T11430 det the,effect
R3171 T11430 T11428 nsubj effect,was
R3172 T11258 T11254 amod right,Figure
R3173 T11432 T11428 acomp subtle,was
R3174 T11433 T11428 prep in,was
R3175 T11259 T11254 punct ),Figure
R3176 T11434 T11433 pobj cell,in
R3177 T11435 T11434 punct (,cell
R3178 T11436 T11434 nummod iii,cell
R3179 T11260 T11220 punct .,localized
R318 T2532 T2533 nsubj Aquaporin,is
R3180 T11437 T11434 punct ),cell
R3181 T11438 T11431 punct ", ",localized
R3182 T11439 T11440 compound AQP2,GFP
R3183 T11262 T11263 compound AQP2,GFP
R3184 T11440 T11431 nsubjpass GFP,localized
R3185 T11441 T11440 punct -,GFP
R3186 T11442 T11440 compound F204V,GFP
R3187 T11443 T11440 punct -,GFP
R3188 T11444 T11431 auxpass was,localized
R3189 T11445 T11431 advmod partly,localized
R319 T2534 T2532 punct -,Aquaporin
R3190 T11446 T11431 prep to,localized
R3191 T11263 T11267 nsubj GFP,showed
R3192 T11447 T11448 det the,surface
R3193 T11448 T11446 pobj surface,to
R3194 T11449 T11448 amod apical,surface
R3195 T11264 T11263 punct -,GFP
R3196 T11450 T11431 punct .,localized
R3197 T11452 T11453 advmod Generally,was
R3198 T11265 T11263 compound F204V,GFP
R3199 T11454 T11453 punct ", ",was
R320 T2535 T2532 nummod 2,Aquaporin
R3200 T11266 T11263 punct -,GFP
R3201 T11455 T11456 det the,localization
R3202 T11456 T11453 nsubj localization,was
R3203 T11457 T11456 prep of,localization
R3204 T11268 T11267 punct ", ",showed
R3205 T11458 T11459 compound AQP2,GFP
R3206 T11459 T11457 pobj GFP,of
R3207 T11460 T11459 punct -,GFP
R3208 T11461 T11459 compound F204V,GFP
R3209 T11269 T11270 advmod when,expressed
R321 T2536 T2532 punct (,Aquaporin
R3210 T11462 T11459 punct -,GFP
R3211 T11463 T11453 advmod clearly,was
R3212 T11464 T11465 advmod more,apical
R3213 T11270 T11267 advcl expressed,showed
R3214 T11465 T11453 acomp apical,was
R3215 T11466 T11467 advmod when,expressed
R3216 T11467 T11453 advcl expressed,was
R3217 T11271 T11270 prep by,expressed
R3218 T11468 T11469 amod wild,type
R3219 T11469 T11471 compound type,AQP2
R322 T2537 T2532 appos AQP2,Aquaporin
R3220 T11470 T11469 punct -,type
R3221 T11272 T11273 amod transient,transfection
R3222 T11471 T11467 nsubjpass AQP2,expressed
R3223 T11472 T11467 auxpass was,expressed
R3224 T11473 T11467 advmod also,expressed
R3225 T11273 T11271 pobj transfection,by
R3226 T11474 T11453 punct .,was
R3227 T11274 T11270 prep into,expressed
R3228 T11476 T11477 aux To,confirm
R3229 T11477 T11478 advcl confirm,transfected
R323 T2538 T2533 punct ),is
R3230 T11275 T11276 npadvmod vector,only
R3231 T11479 T11480 det these,results
R3232 T11480 T11477 dobj results,confirm
R3233 T11276 T11277 amod only,cells
R3234 T11481 T11477 advmod biochemically,confirm
R3235 T11482 T11478 punct ", ",transfected
R3236 T11483 T11478 nsubj we,transfected
R3237 T11277 T11274 pobj cells,into
R3238 T11484 T11485 det the,lines
R3239 T11485 T11478 dobj lines,transfected
R324 T2539 T2540 det a,protein
R3240 T11486 T11485 amod same,lines
R3241 T11487 T11485 compound cell,lines
R3242 T11488 T11485 punct (,lines
R3243 T11278 T11267 punct ", ",showed
R3244 T11489 T11490 amod wild,type
R3245 T11490 T11492 compound type,AQP2
R3246 T11491 T11490 punct -,type
R3247 T11279 T11280 det a,pattern
R3248 T11492 T11485 appos AQP2,lines
R3249 T11493 T11492 cc or,AQP2
R325 T2540 T2533 attr protein,is
R3250 T11494 T11492 conj vector,AQP2
R3251 T11280 T11267 dobj pattern,showed
R3252 T11495 T11485 punct ),lines
R3253 T11496 T11478 prep with,transfected
R3254 T11497 T11498 compound F204V,GFP
R3255 T11281 T11280 amod diffuse,pattern
R3256 T11498 T11496 pobj GFP,with
R3257 T11499 T11498 punct -,GFP
R3258 T11500 T11478 punct ", ",transfected
R3259 T11282 T11280 amod cytoplasmic,pattern
R326 T2541 T2542 npadvmod pore,forming
R3260 T11501 T11478 conj biotinylated,transfected
R3261 T11283 T11280 compound distribution,pattern
R3262 T11502 T11503 compound surface,proteins
R3263 T11284 T11285 punct (,left
R3264 T11503 T11501 dobj proteins,biotinylated
R3265 T11504 T11501 prep after,biotinylated
R3266 T11505 T11506 compound forskolin,stimulation
R3267 T11285 T11267 parataxis left,showed
R3268 T11506 T11504 pobj stimulation,after
R3269 T11507 T11501 punct ", ",biotinylated
R327 T2542 T2540 amod forming,protein
R3270 T11286 T11285 dep Figure,left
R3271 T11508 T11501 cc and,biotinylated
R3272 T11509 T11501 conj precipitated,biotinylated
R3273 T11510 T11511 det the,proteins
R3274 T11287 T11286 nummod 5C,Figure
R3275 T11511 T11509 dobj proteins,precipitated
R3276 T11512 T11511 amod biotinylated,proteins
R3277 T11288 T11285 punct ", ",left
R3278 T11513 T11514 punct (,Figure
R3279 T11514 T11509 parataxis Figure,precipitated
R328 T2543 T2542 punct -,forming
R3280 T11515 T11514 nummod 5D,Figure
R3281 T11289 T11285 amod lower,left
R3282 T11516 T11514 punct ),Figure
R3283 T11517 T11478 punct .,transfected
R3284 T11290 T11285 punct ),left
R3285 T11291 T11267 punct .,showed
R3286 T11293 T11294 advmod When,expressed
R3287 T11519 T11520 compound AQP2,GFP
R3288 T11520 T11524 nsubjpass GFP,expressed
R3289 T11294 T11295 advcl expressed,localized
R329 T2544 T2540 acl belonging,protein
R3290 T11521 T11520 punct -,GFP
R3291 T11522 T11520 compound F204V,GFP
R3292 T11523 T11520 punct -,GFP
R3293 T11525 T11524 auxpass is,expressed
R3294 T11296 T11294 prep in,expressed
R3295 T11526 T11527 advmod approximately,equally
R3296 T11527 T11524 advmod equally,expressed
R3297 T11528 T11524 prep in,expressed
R3298 T11529 T11530 det both,lines
R3299 T11530 T11528 pobj lines,in
R330 T2545 T2544 prep to,belonging
R3300 T11297 T11298 amod wild,type
R3301 T11531 T11530 compound cell,lines
R3302 T11532 T11533 punct (,cells
R3303 T11533 T11524 parataxis cells,expressed
R3304 T11298 T11300 compound type,cells
R3305 T11534 T11533 dep Figure,cells
R3306 T11535 T11534 nummod 5D,Figure
R3307 T11536 T11533 punct ", ",cells
R3308 T11299 T11298 punct -,type
R3309 T11537 T11533 amod total,cells
R331 T2546 T2547 det a,family
R3310 T11538 T11533 punct ),cells
R3311 T11300 T11296 pobj cells,in
R3312 T11539 T11524 punct ", ",expressed
R3313 T11540 T11524 cc but,expressed
R3314 T11541 T11542 auxpass is,biotinylated
R3315 T11301 T11300 compound AQP2,cells
R3316 T11542 T11524 conj biotinylated,expressed
R3317 T11543 T11544 advmod only,expressed
R3318 T11544 T11542 ccomp expressed,biotinylated
R3319 T11302 T11295 punct ", ",localized
R332 T2547 T2545 pobj family,to
R3320 T11545 T11544 advmod when,expressed
R3321 T11546 T11547 amod wild,type
R3322 T11547 T11549 compound type,AQP2
R3323 T11303 T11295 advmod however,localized
R3324 T11548 T11547 punct -,type
R3325 T11549 T11544 nsubjpass AQP2,expressed
R3326 T11550 T11544 auxpass is,expressed
R3327 T11304 T11295 punct ", ",localized
R3328 T11551 T11544 advmod also,expressed
R3329 T11552 T11553 punct (,Figure
R333 T2548 T2547 prep of,family
R3330 T11553 T11542 parataxis Figure,biotinylated
R3331 T11305 T11306 compound AQP2,GFP
R3332 T11554 T11553 nummod 5D,Figure
R3333 T11555 T11553 punct ", ",Figure
R3334 T11556 T11557 npadvmod surface,biotinylated
R3335 T11306 T11295 nsubj GFP,localized
R3336 T11557 T11553 amod biotinylated,Figure
R3337 T11558 T11553 punct ),Figure
R3338 T11559 T11524 punct .,expressed
R3339 T11307 T11306 punct -,GFP
R334 T2549 T2550 compound water,channels
R3340 T11561 T11562 mark Since,are
R3341 T11562 T11567 advcl are,indicate
R3342 T11563 T11564 advmod only,proteins
R3343 T11564 T11562 nsubj proteins,are
R3344 T11565 T11566 compound cell,surface
R3345 T11566 T11564 compound surface,proteins
R3346 T11568 T11562 acomp accessible,are
R3347 T11569 T11568 prep to,accessible
R3348 T11570 T11569 pobj biotin,to
R3349 T11571 T11567 punct ", ",indicate
R335 T2550 T2548 pobj channels,of
R3350 T11572 T11573 det these,results
R3351 T11573 T11567 nsubj results,indicate
R3352 T11574 T11575 mark that,transported
R3353 T11575 T11567 ccomp transported,indicate
R3354 T11576 T11577 compound AQP2,F204V
R3355 T11577 T11575 nsubjpass F204V,transported
R3356 T11578 T11577 punct -,F204V
R3357 T11579 T11575 auxpass is,transported
R3358 T11580 T11575 prep to,transported
R3359 T11581 T11582 det the,surface
R336 T2551 T2552 punct [,4
R3360 T11582 T11580 pobj surface,to
R3361 T11583 T11582 compound cell,surface
R3362 T11584 T11585 advmod when,is
R3363 T11585 T11575 advcl is,transported
R3364 T11586 T11587 amod wild,type
R3365 T11587 T11589 compound type,AQP2
R3366 T11588 T11587 punct -,type
R3367 T11589 T11585 nsubj AQP2,is
R3368 T11590 T11585 acomp present,is
R3369 T11591 T11585 punct ", ",is
R337 T2552 T2533 parataxis 4,is
R3370 T11592 T11585 cc but,is
R3371 T11593 T11592 neg not,but
R3372 T11594 T11585 conj on,is
R3373 T11595 T11596 poss its,own
R3374 T11596 T11594 pobj own,on
R3375 T11597 T11567 punct .,indicate
R3378 T14172 T14173 compound Aqp2F204,F204V
R3379 T14173 T14175 compound F204V,mice
R338 T2553 T2552 punct ],4
R3380 T14174 T14173 punct /,F204V
R3381 T14175 T14176 nsubj mice,are
R3382 T14177 T14176 acomp viable,are
R3383 T14178 T14176 cc and,are
R3384 T14179 T14176 conj grow,are
R3385 T14180 T14179 cc and,grow
R3386 T14181 T14179 conj reproduce,grow
R3387 T14182 T14181 advmod normally,reproduce
R3388 T14183 T14176 punct .,are
R3389 T14185 T14186 nsubj They,are
R339 T2554 T2533 punct ", ",is
R3390 T14187 T14186 punct ", ",are
R3391 T14188 T14186 advmod however,are
R3392 T14189 T14186 punct ", ",are
R3393 T14190 T14191 advmod severely,defective
R3394 T14191 T14186 acomp defective,are
R3395 T14192 T14191 prep in,defective
R3396 T14193 T14194 poss their,ability
R3397 T14194 T14192 pobj ability,in
R3398 T14195 T14196 aux to,concentrate
R3399 T14196 T14194 acl concentrate,ability
R340 T2555 T2533 cc and,is
R3400 T14197 T14196 dobj urine,concentrate
R3401 T14198 T14186 punct ", ",are
R3402 T14199 T14186 advcl leading,are
R3403 T14200 T14199 prep to,leading
R3404 T14201 T14202 amod increased,output
R3405 T14202 T14200 pobj output,to
R3406 T14203 T14202 compound urine,output
R3407 T14204 T14202 cc and,output
R3408 T14205 T14206 compound water,intake
R3409 T14206 T14202 conj intake,output
R341 T2556 T2557 nsubjpass it,expressed
R3410 T14207 T14186 punct ", ",are
R3411 T14208 T14209 advmod thus,making
R3412 T14209 T14186 advcl making,are
R3413 T14210 T14211 nsubj them,model
R3414 T14211 T14209 ccomp model,making
R3415 T14212 T14211 det the,model
R3416 T14213 T14211 amod first,model
R3417 T14214 T14211 compound mouse,model
R3418 T14215 T14211 prep of,model
R3419 T14216 T14215 pobj NDI,of
R342 T2557 T2533 conj expressed,is
R3420 T14217 T14218 aux to,survive
R3421 T14218 T14211 advcl survive,model
R3422 T14219 T14218 prep to,survive
R3423 T14220 T14219 pobj maturity,to
R3424 T14221 T14186 punct .,are
R3425 T14223 T14224 prep In,caused
R3426 T14225 T14223 pobj humans,In
R3427 T14226 T14224 punct ", ",caused
R3428 T14227 T14224 nsubjpass NDI,caused
R3429 T14228 T14224 auxpass is,caused
R343 T2558 T2557 auxpass is,expressed
R3430 T14229 T14224 agent by,caused
R3431 T14230 T14229 pobj mutations,by
R3432 T14231 T14230 prep in,mutations
R3433 T14232 T14231 pobj Avpr2,in
R3434 T14233 T14232 cc or,Avpr2
R3435 T14234 T14232 conj Aqp2,Avpr2
R3436 T14235 T14224 punct .,caused
R3437 T14237 T14238 nsubj Knockout,gave
R3438 T14239 T14237 prep of,Knockout
R3439 T14240 T14241 det the,gene
R344 T2559 T2557 prep in,expressed
R3440 T14241 T14239 pobj gene,of
R3441 T14242 T14243 npadvmod X,linked
R3442 T14243 T14241 amod linked,gene
R3443 T14244 T14243 punct -,linked
R3444 T14245 T14241 compound Avpr2,gene
R3445 T14246 T14237 prep in,Knockout
R3446 T14247 T14246 pobj mice,in
R3447 T14248 T14249 punct [,15
R3448 T14249 T14237 parataxis 15,Knockout
R3449 T14250 T14249 punct ],15
R345 T2560 T2561 amod collecting,duct
R3450 T14251 T14252 det an,phenotype
R3451 T14252 T14238 dobj phenotype,gave
R3452 T14253 T14254 npadvmod NDI,like
R3453 T14254 T14252 amod like,phenotype
R3454 T14255 T14254 punct -,like
R3455 T14256 T14238 prep in,gave
R3456 T14257 T14258 amod male,neonates
R3457 T14258 T14256 pobj neonates,in
R3458 T14259 T14258 punct ", ",neonates
R3459 T14260 T14258 amod hemizygous,neonates
R346 T2561 T2563 nmod duct,cells
R3460 T14261 T14238 punct ", ",gave
R3461 T14262 T14238 cc but,gave
R3462 T14263 T14264 det the,phenotype
R3463 T14264 T14265 nsubjpass phenotype,assessed
R3464 T14265 T14238 conj assessed,gave
R3465 T14266 T14265 aux could,assessed
R3466 T14267 T14265 neg not,assessed
R3467 T14268 T14265 auxpass be,assessed
R3468 T14269 T14265 prep in,assessed
R3469 T14270 T14269 pobj adults,in
R347 T2562 T2561 punct -,duct
R3470 T14271 T14272 mark as,died
R3471 T14272 T14265 advcl died,assessed
R3472 T14273 T14274 det the,mice
R3473 T14274 T14272 nsubj mice,died
R3474 T14275 T14272 prep within,died
R3475 T14276 T14277 nummod 1,wk
R3476 T14277 T14275 pobj wk,within
R3477 T14278 T14277 prep of,wk
R3478 T14279 T14278 pobj birth,of
R3479 T14280 T14265 punct .,assessed
R348 T2563 T2559 pobj cells,in
R3480 T14282 T14283 det The,females
R3481 T14283 T14286 nsubj females,showed
R3482 T14284 T14283 amod adult,females
R3483 T14285 T14283 amod heterozygous,females
R3484 T14287 T14288 det a,tendency
R3485 T14288 T14286 dobj tendency,showed
R3486 T14289 T14288 amod mild,tendency
R3487 T14290 T14288 prep toward,tendency
R3488 T14291 T14292 amod increased,output
R3489 T14292 T14290 pobj output,toward
R349 T2564 T2563 amod principal,cells
R3490 T14293 T14292 amod urinary,output
R3491 T14294 T14292 cc and,output
R3492 T14295 T14296 compound water,intake
R3493 T14296 T14292 conj intake,output
R3494 T14297 T14292 cc and,output
R3495 T14298 T14299 amod decreased,osmolality
R3496 T14299 T14292 conj osmolality,output
R3497 T14300 T14299 compound urine,osmolality
R3498 T14301 T14286 punct .,showed
R3499 T14303 T14304 nsubjpass Knockout,reported
R350 T2565 T2557 prep in,expressed
R3500 T14305 T14303 prep of,Knockout
R3501 T14306 T14307 det the,gene
R3502 T14307 T14305 pobj gene,of
R3503 T14308 T14307 compound mouse,gene
R3504 T14309 T14307 compound Aqp2,gene
R3505 T14310 T14304 aux has,reported
R3506 T14311 T14304 neg not,reported
R3507 T14312 T14304 auxpass been,reported
R3508 T14313 T14304 punct .,reported
R3509 T14315 T14316 det A,in
R351 T2566 T2567 det the,kidney
R3510 T14316 T14319 nsubjpass in,made
R3511 T14317 T14316 compound knock,in
R3512 T14318 T14316 punct -,in
R3513 T14320 T14316 prep of,in
R3514 T14321 T14322 det a,mutation
R3515 T14322 T14320 pobj mutation,of
R3516 T14323 T14322 amod human,mutation
R3517 T14324 T14325 npadvmod disease,causing
R3518 T14325 T14322 amod causing,mutation
R3519 T14326 T14325 punct -,causing
R352 T2567 T2565 pobj kidney,in
R3520 T14327 T14322 punct (,mutation
R3521 T14328 T14322 appos T126M,mutation
R3522 T14329 T14322 punct ),mutation
R3523 T14330 T14319 punct ", ",made
R3524 T14331 T14319 advmod however,made
R3525 T14332 T14319 punct ", ",made
R3526 T14333 T14319 aux has,made
R3527 T14334 T14319 auxpass been,made
R3528 T14335 T14336 punct [,18
R3529 T14336 T14319 parataxis 18,made
R353 T2568 T2569 punct [,5
R3530 T14337 T14336 punct ],18
R3531 T14338 T14319 punct .,made
R3532 T14340 T14341 det These,mice
R3533 T14341 T14342 nsubj mice,have
R3534 T14343 T14344 det a,defect
R3535 T14344 T14342 dobj defect,have
R3536 T14345 T14344 amod severe,defect
R3537 T14346 T14347 npadvmod urine,concentrating
R3538 T14347 T14344 amod concentrating,defect
R3539 T14348 T14347 punct -,concentrating
R354 T2569 T2557 parataxis 5,expressed
R3540 T14349 T14344 acl resulting,defect
R3541 T14350 T14349 prep in,resulting
R3542 T14351 T14350 pobj dehydration,in
R3543 T14352 T14351 cc and,dehydration
R3544 T14353 T14351 conj death,dehydration
R3545 T14354 T14349 prep within,resulting
R3546 T14355 T14356 nummod 1,wk
R3547 T14356 T14354 pobj wk,within
R3548 T14357 T14356 prep of,wk
R3549 T14358 T14357 pobj birth,of
R355 T2570 T2569 punct ],5
R3550 T14359 T14342 punct .,have
R3551 T14361 T14362 advmod Curiously,localize
R3552 T14363 T14362 punct ", ",localize
R3553 T14364 T14365 compound AQP2,T126M
R3554 T14365 T14362 nsubj T126M,localize
R3555 T14366 T14365 punct -,T126M
R3556 T14367 T14362 aux does,localize
R3557 T14368 T14362 advmod properly,localize
R3558 T14369 T14362 prep in,localize
R3559 T14370 T14371 advmod at,least
R356 T2571 T2557 punct .,expressed
R3560 T14371 T14372 advmod least,subset
R3561 T14372 T14369 pobj subset,in
R3562 T14373 T14372 det a,subset
R3563 T14374 T14372 prep of,subset
R3564 T14375 T14374 pobj cells,of
R3565 T14376 T14362 punct .,localize
R3566 T14378 T14379 det The,morphology
R3567 T14379 T14384 nsubj morphology,makes
R3568 T14380 T14381 advmod grossly,abnormal
R3569 T14381 T14379 amod abnormal,morphology
R357 T2573 T2574 advmod Generally,permit
R3570 T14382 T14383 amod collecting,duct
R3571 T14383 T14379 compound duct,morphology
R3572 T14385 T14386 nsubj it,impossible
R3573 T14386 T14384 ccomp impossible,makes
R3574 T14387 T14388 aux to,pinpoint
R3575 T14388 T14386 advcl pinpoint,impossible
R3576 T14389 T14390 det the,defect
R3577 T14390 T14388 dobj defect,pinpoint
R3578 T14391 T14390 amod molecular,defect
R3579 T14392 T14388 prep in,pinpoint
R358 T2575 T2576 det these,proteins
R3580 T14393 T14394 det these,mice
R3581 T14394 T14392 pobj mice,in
R3582 T14395 T14394 amod knock,mice
R3583 T14396 T14395 punct -,knock
R3584 T14397 T14395 prt in,knock
R3585 T14398 T14384 punct .,makes
R3586 T14400 T14401 det The,in
R3587 T14401 T14405 nsubj in,shows
R3588 T14402 T14401 compound T126M,in
R3589 T14403 T14401 compound knock,in
R359 T2576 T2574 nsubj proteins,permit
R3590 T14404 T14401 punct -,in
R3591 T14406 T14405 advmod clearly,shows
R3592 T14407 T14408 mark that,is
R3593 T14408 T14405 ccomp is,shows
R3594 T14409 T14408 nsubj Aqp2,is
R3595 T14410 T14411 det an,gene
R3596 T14411 T14408 attr gene,is
R3597 T14412 T14411 amod essential,gene
R3598 T14413 T14414 punct [,18
R3599 T14414 T14405 parataxis 18,shows
R360 T2577 T2578 det the,passage
R3600 T14415 T14414 punct ],18
R3601 T14416 T14405 punct .,shows
R3602 T14418 T14419 det The,fact
R3603 T14419 T14420 nsubj fact,shows
R3604 T14421 T14422 mark that,survive
R3605 T14422 T14419 acl survive,fact
R3606 T14423 T14424 poss our,mice
R3607 T14424 T14422 nsubj mice,survive
R3608 T14425 T14426 preconj either,possesses
R3609 T14426 T14420 advcl possesses,shows
R361 T2578 T2574 dobj passage,permit
R3610 T14427 T14426 mark that,possesses
R3611 T14428 T14429 compound AQP2,F204V
R3612 T14429 T14426 nsubj F204V,possesses
R3613 T14430 T14429 punct -,F204V
R3614 T14431 T14432 det some,ability
R3615 T14432 T14426 dobj ability,possesses
R3616 T14433 T14432 amod residual,ability
R3617 T14434 T14435 npadvmod water,transporting
R3618 T14435 T14432 amod transporting,ability
R3619 T14436 T14426 cc or,possesses
R362 T2579 T2578 prep of,passage
R3620 T14437 T14438 mark that,are
R3621 T14438 T14426 conj are,possesses
R3622 T14439 T14438 expl there,are
R3623 T14440 T14441 npadvmod AVP,independent
R3624 T14441 T14443 amod independent,pathways
R3625 T14442 T14441 punct -,independent
R3626 T14443 T14438 attr pathways,are
R3627 T14444 T14443 prep for,pathways
R3628 T14445 T14446 compound water,reabsorption
R3629 T14446 T14444 pobj reabsorption,for
R363 T2580 T2579 pobj water,of
R3630 T14447 T14420 punct .,shows
R3631 T14449 T14450 amod Residual,activity
R3632 T14450 T14451 nsubj activity,is
R3633 T14452 T14450 prep of,activity
R3634 T14453 T14454 compound AQP2,F204V
R3635 T14454 T14452 pobj F204V,of
R3636 T14455 T14454 punct -,F204V
R3637 T14456 T14451 acomp likely,is
R3638 T14457 T14451 punct ", ",is
R3639 T14458 T14459 mark as,show
R364 T2581 T2578 prep through,passage
R3640 T14459 T14451 advcl show,is
R3641 T14460 T14461 compound mutant,animals
R3642 T14461 T14459 nsubj animals,show
R3643 T14462 T14463 det some,response
R3644 T14463 T14459 dobj response,show
R3645 T14464 T14463 amod small,response
R3646 T14465 T14463 prep to,response
R3647 T14466 T14465 pobj dDAVP,to
R3648 T14467 T14459 punct ", ",show
R3649 T14468 T14469 mark although,remains
R365 T2582 T2583 det the,membrane
R3650 T14469 T14459 advcl remains,show
R3651 T14470 T14471 npadvmod dDAVP,stimulated
R3652 T14471 T14473 amod stimulated,osmolality
R3653 T14472 T14471 punct -,stimulated
R3654 T14473 T14469 nsubj osmolality,remains
R3655 T14474 T14473 compound urine,osmolality
R3656 T14475 T14476 advmod quite,low
R3657 T14476 T14469 acomp low,remains
R3658 T14477 T14451 punct .,is
R3659 T14479 T14480 nsubj Immunostaining,shows
R366 T2583 T2581 pobj membrane,through
R3660 T14481 T14479 prep of,Immunostaining
R3661 T14482 T14481 pobj kidney,of
R3662 T14483 T14484 mark that,transport
R3663 T14484 T14480 ccomp transport,shows
R3664 T14485 T14486 compound AQP2,F204V
R3665 T14486 T14484 nsubj F204V,transport
R3666 T14487 T14486 punct -,F204V
R3667 T14488 T14484 aux does,transport
R3668 T14489 T14484 neg not,transport
R3669 T14490 T14484 advmod efficiently,transport
R367 T2584 T2583 compound plasma,membrane
R3670 T14491 T14484 dobj water,transport
R3671 T14492 T14484 punct ", ",transport
R3672 T14493 T14494 mark because,fails
R3673 T14494 T14484 advcl fails,transport
R3674 T14495 T14494 nsubj it,fails
R3675 T14496 T14497 aux to,localize
R3676 T14497 T14494 xcomp localize,fails
R3677 T14498 T14497 prep to,localize
R3678 T14499 T14500 det the,surface
R3679 T14500 T14498 pobj surface,to
R368 T2585 T2583 punct (,membrane
R3680 T14501 T14500 amod apical,surface
R3681 T14502 T14500 compound cell,surface
R3682 T14503 T14497 prep after,localize
R3683 T14504 T14505 compound dDAVP,treatment
R3684 T14505 T14503 pobj treatment,after
R3685 T14506 T14480 punct .,shows
R3686 T14508 T14509 det Some,activity
R3687 T14509 T14511 nsubj activity,imply
R3688 T14510 T14509 amod residual,activity
R3689 T14512 T14509 prep of,activity
R369 T2586 T2583 appos PM,membrane
R3690 T14513 T14512 pobj AQP2,of
R3691 T14514 T14511 aux would,imply
R3692 T14515 T14516 mark that,getting
R3693 T14516 T14511 ccomp getting,imply
R3694 T14517 T14518 det some,portion
R3695 T14518 T14516 nsubj portion,getting
R3696 T14519 T14518 amod small,portion
R3697 T14520 T14518 punct ", ",portion
R3698 T14521 T14518 amod undetectable,portion
R3699 T14522 T14518 prep of,portion
R370 T2587 T2583 punct ),membrane
R3700 T14523 T14524 det the,protein
R3701 T14524 T14522 pobj protein,of
R3702 T14525 T14524 compound mutant,protein
R3703 T14526 T14516 aux is,getting
R3704 T14527 T14516 prep to,getting
R3705 T14528 T14529 det the,surface
R3706 T14529 T14527 pobj surface,to
R3707 T14530 T14529 compound cell,surface
R3708 T14531 T14511 punct .,imply
R3709 T14533 T14534 det The,experiment
R371 T2588 T2583 prep of,membrane
R3710 T14534 T14537 nsubj experiment,suggests
R3711 T14535 T14534 compound surface,experiment
R3712 T14536 T14534 compound biotinylation,experiment
R3713 T14538 T14539 punct (,Figure
R3714 T14539 T14534 parataxis Figure,experiment
R3715 T14540 T14539 nummod 5D,Figure
R3716 T14541 T14539 punct ),Figure
R3717 T14542 T14543 mark that,gets
R3718 T14543 T14537 ccomp gets,suggests
R3719 T14544 T14545 det no,protein
R372 T2589 T2588 pobj cells,of
R3720 T14545 T14543 nsubj protein,gets
R3721 T14546 T14545 compound mutant,protein
R3722 T14547 T14543 prep to,gets
R3723 T14548 T14549 det the,surface
R3724 T14549 T14547 pobj surface,to
R3725 T14550 T14537 punct ", ",suggests
R3726 T14551 T14537 cc but,suggests
R3727 T14552 T14553 nsubj this,reflect
R3728 T14553 T14537 conj reflect,suggests
R3729 T14554 T14553 aux does,reflect
R373 T2590 T2589 punct ", ",cells
R3730 T14555 T14553 neg not,reflect
R3731 T14556 T14553 advmod necessarily,reflect
R3732 T14557 T14558 det the,situation
R3733 T14558 T14553 dobj situation,reflect
R3734 T14559 T14560 advmod in,vivo
R3735 T14560 T14558 advmod vivo,situation
R3736 T14561 T14553 punct .,reflect
R3737 T14563 T14564 mark While,be
R3738 T14564 T14572 advcl be,shows
R3739 T14565 T14566 det this,fraction
R374 T2591 T2592 dep several,carry
R3740 T14566 T14564 nsubj fraction,be
R3741 T14567 T14566 amod small,fraction
R3742 T14568 T14566 prep of,fraction
R3743 T14569 T14568 pobj protein,of
R3744 T14570 T14564 aux may,be
R3745 T14571 T14564 neg not,be
R3746 T14573 T14564 acomp detectable,be
R3747 T14574 T14564 prep by,be
R3748 T14575 T14574 pobj immunofluorescence,by
R3749 T14576 T14572 punct ", ",shows
R375 T2592 T2589 relcl carry,cells
R3750 T14577 T14578 compound Western,blotting
R3751 T14578 T14572 nsubj blotting,shows
R3752 T14579 T14580 mark that,progress
R3753 T14580 T14572 ccomp progress,shows
R3754 T14581 T14582 det some,protein
R3755 T14582 T14580 nsubj protein,progress
R3756 T14583 T14582 compound mutant,protein
R3757 T14584 T14580 aux does,progress
R3758 T14585 T14580 prep beyond,progress
R3759 T14586 T14587 det the,ER
R376 T2593 T2591 prep of,several
R3760 T14587 T14585 pobj ER,beyond
R3761 T14588 T14589 punct (,species
R3762 T14589 T14572 parataxis species,shows
R3763 T14590 T14591 quantmod 35,45
R3764 T14591 T14593 nummod 45,kDa
R3765 T14592 T14591 punct –,45
R3766 T14593 T14589 compound kDa,species
R3767 T14594 T14589 prep in,species
R3768 T14595 T14594 pobj Figure,in
R3769 T14596 T14595 nummod 3A,Figure
R377 T2594 T2593 pobj which,of
R3770 T14597 T14589 punct ),species
R3771 T14598 T14572 punct .,shows
R3772 T14600 T14601 prep Compared,is
R3773 T14602 T14600 prep to,Compared
R3774 T14603 T14604 amod wild,type
R3775 T14604 T14602 pobj type,to
R3776 T14605 T14604 punct -,type
R3777 T14606 T14601 punct ", ",is
R3778 T14607 T14608 compound mutant,protein
R3779 T14608 T14601 nsubj protein,is
R378 T2595 T2592 prt out,carry
R3780 T14609 T14601 acomp enriched,is
R3781 T14610 T14609 prep in,enriched
R3782 T14611 T14612 det the,form
R3783 T14612 T14610 pobj form,in
R3784 T14613 T14614 amod high,mannose
R3785 T14614 T14612 nmod mannose,form
R3786 T14615 T14614 punct -,mannose
R3787 T14616 T14612 punct ", ",form
R3788 T14617 T14618 npadvmod core,glycosylated
R3789 T14618 T14612 amod glycosylated,form
R379 T2596 T2597 det this,role
R3790 T14619 T14618 punct -,glycosylated
R3791 T14620 T14621 punct (,kDa
R3792 T14621 T14612 parataxis kDa,form
R3793 T14622 T14621 nummod 31,kDa
R3794 T14623 T14621 punct ),kDa
R3795 T14624 T14609 cc and,enriched
R3796 T14625 T14609 conj deficient,enriched
R3797 T14626 T14625 prep in,deficient
R3798 T14627 T14628 amod nonglycosylated,forms
R3799 T14628 T14626 pobj forms,in
R380 T2597 T2592 dobj role,carry
R3800 T14629 T14630 punct (,kDa
R3801 T14630 T14627 parataxis kDa,nonglycosylated
R3802 T14631 T14630 nummod 29,kDa
R3803 T14632 T14630 punct ),kDa
R3804 T14633 T14627 cc and,nonglycosylated
R3805 T14634 T14635 amod complex,glycosylated
R3806 T14635 T14627 conj glycosylated,nonglycosylated
R3807 T14636 T14637 punct (,kDa
R3808 T14637 T14635 parataxis kDa,glycosylated
R3809 T14638 T14639 quantmod 35,45
R381 T2598 T2599 advmod specifically,in
R3810 T14639 T14637 nummod 45,kDa
R3811 T14640 T14639 punct –,45
R3812 T14641 T14637 punct ),kDa
R3813 T14642 T14601 punct .,is
R3814 T14644 T14645 det The,presence
R3815 T14645 T14646 nsubj presence,indicates
R3816 T14647 T14645 prep of,presence
R3817 T14648 T14649 det a,amount
R3818 T14649 T14647 pobj amount,of
R3819 T14650 T14649 amod reduced,amount
R382 T2599 T2592 prep in,carry
R3820 T14651 T14650 cc but,reduced
R3821 T14652 T14650 conj detectable,reduced
R3822 T14653 T14649 prep of,amount
R3823 T14654 T14653 pobj protein,of
R3824 T14655 T14654 prep in,protein
R3825 T14656 T14657 det the,range
R3826 T14657 T14655 pobj range,in
R3827 T14658 T14659 quantmod 35,45
R3828 T14659 T14661 nummod 45,kDa
R3829 T14660 T14659 punct –,45
R383 T2600 T2601 det the,process
R3830 T14661 T14657 compound kDa,range
R3831 T14662 T14663 mark that,transported
R3832 T14663 T14646 ccomp transported,indicates
R3833 T14664 T14665 compound mutant,protein
R3834 T14665 T14663 nsubjpass protein,transported
R3835 T14666 T14663 auxpass is,transported
R3836 T14667 T14663 prep out,transported
R3837 T14668 T14667 prep of,out
R3838 T14669 T14670 det the,ER
R3839 T14670 T14668 pobj ER,of
R384 T2601 T2599 pobj process,in
R3840 T14671 T14663 punct ", ",transported
R3841 T14672 T14673 cc but,with
R3842 T14673 T14663 prep with,transported
R3843 T14674 T14675 advmod greatly,reduced
R3844 T14675 T14676 amod reduced,efficiency
R3845 T14676 T14673 pobj efficiency,with
R3846 T14677 T14646 punct .,indicates
R3847 T14679 T14680 nsubj Colocalization,shows
R3848 T14681 T14679 prep of,Colocalization
R3849 T14682 T14683 compound AQP2,F204V
R385 T2602 T2601 prep of,process
R3850 T14683 T14681 pobj F204V,of
R3851 T14684 T14683 punct -,F204V
R3852 T14685 T14679 prep with,Colocalization
R3853 T14686 T14687 det the,protein
R3854 T14687 T14685 pobj protein,with
R3855 T14688 T14687 compound ER,protein
R3856 T14689 T14687 appos calnexin,protein
R3857 T14690 T14679 prep in,Colocalization
R3858 T14691 T14692 amod transfected,cells
R3859 T14692 T14690 pobj cells,in
R386 T2603 T2604 compound water,reabsorption
R3860 T14693 T14692 compound MDCK,cells
R3861 T14694 T14695 mark that,progress
R3862 T14695 T14680 ccomp progress,shows
R3863 T14696 T14695 punct ", ",progress
R3864 T14697 T14698 mark while,trapped
R3865 T14698 T14695 advcl trapped,progress
R3866 T14738 T14737 prep of,fraction
R3867 T14699 T14698 nsubjpass most,trapped
R3868 T14739 T14740 compound mutant,protein
R3869 T14700 T14699 prep of,most
R387 T2604 T2602 pobj reabsorption,of
R3870 T14740 T14738 pobj protein,of
R3871 T14741 T14734 prep beyond,transport
R3872 T14701 T14702 det the,protein
R3873 T14742 T14743 det the,ER
R3874 T14743 T14741 pobj ER,beyond
R3875 T14744 T14734 prep in,transport
R3876 T14702 T14700 pobj protein,of
R3877 T14745 T14746 compound MDCK,cells
R3878 T14746 T14744 pobj cells,in
R3879 T14747 T14718 advmod all,are
R388 T2605 T2604 prep from,reabsorption
R3880 T14703 T14702 compound mutant,protein
R3881 T14748 T14718 acomp consistent,are
R3882 T14749 T14748 prep with,consistent
R3883 T14750 T14751 det the,notion
R3884 T14704 T14698 auxpass is,trapped
R3885 T14751 T14749 pobj notion,with
R3886 T14752 T14753 mark that,is
R3887 T14753 T14751 advcl is,notion
R3888 T14705 T14698 prep in,trapped
R3889 T14754 T14755 compound AQP2,F204V
R389 T2606 T2605 pobj urine,from
R3890 T14755 T14757 compound F204V,misfolding
R3891 T14756 T14755 punct -,F204V
R3892 T14706 T14707 det the,ER
R3893 T14757 T14753 nsubj misfolding,is
R3894 T14758 T14753 acomp limited,is
R3895 T14759 T14753 cc and,is
R3896 T14760 T14761 mark that,retain
R3897 T14761 T14753 conj retain,is
R3898 T14762 T14761 nsubj it,retain
R3899 T14707 T14705 pobj ER,in
R390 T2607 T2604 prep in,reabsorption
R3900 T14763 T14761 aux may,retain
R3901 T14764 T14765 det some,activity
R3902 T14765 T14761 dobj activity,retain
R3903 T14708 T14695 punct ", ",progress
R3904 T14766 T14765 amod residual,activity
R3905 T14767 T14768 npadvmod water,transporting
R3906 T14709 T14695 nsubj some,progress
R3907 T14768 T14765 amod transporting,activity
R3908 T14769 T14718 punct .,are
R3909 T14771 T14772 advmod Evidently,is
R391 T2608 T2609 det the,kidney
R3910 T14710 T14695 aux does,progress
R3911 T14773 T14774 det this,activity
R3912 T14774 T14772 nsubj activity,is
R3913 T14711 T14695 prep beyond,progress
R3914 T14775 T14774 amod residual,activity
R3915 T14712 T14713 det the,ER
R3916 T14776 T14772 acomp sufficient,is
R3917 T14777 T14776 prep for,sufficient
R3918 T14713 T14711 pobj ER,beyond
R3919 T14778 T14779 det the,viability
R392 T2609 T2607 pobj kidney,in
R3920 T14779 T14777 pobj viability,for
R3921 T14780 T14779 cc and,viability
R3922 T14714 T14680 punct .,shows
R3923 T14781 T14779 conj growth,viability
R3924 T14782 T14779 prep of,viability
R3925 T14783 T14784 compound mutant,animals
R3926 T14716 T14717 amod Diminished,response
R3927 T14784 T14782 pobj animals,of
R3928 T14785 T14772 punct .,is
R3929 T14787 T14788 amod Reduced,efficiency
R393 T2610 T2574 punct .,permit
R3930 T14717 T14718 nsubj response,are
R3931 T14788 T14789 nsubj efficiency,explain
R3932 T14790 T14788 prep in,efficiency
R3933 T14719 T14717 prep to,response
R3934 T14791 T14790 pcomp exiting,in
R3935 T14792 T14793 det the,ER
R3936 T14793 T14791 dobj ER,exiting
R3937 T14794 T14789 aux may,explain
R3938 T14720 T14719 pobj dDAVP,to
R3939 T14795 T14796 advmod why,enriched
R394 T2612 T2613 nsubjpass It,established
R3940 T14796 T14789 ccomp enriched,explain
R3941 T14797 T14798 compound AQP2,F204V
R3942 T14721 T14717 punct ", ",response
R3943 T14798 T14796 nsubjpass F204V,enriched
R3944 T14799 T14798 punct -,F204V
R3945 T14800 T14796 auxpass is,enriched
R3946 T14801 T14796 prep in,enriched
R3947 T14802 T14803 det the,form
R3948 T14803 T14801 pobj form,in
R3949 T14722 T14723 amod diminished,abundance
R395 T2614 T2613 aux has,established
R3950 T14804 T14805 nummod 31,kDa
R3951 T14805 T14803 nmod kDa,form
R3952 T14806 T14807 amod high,mannose
R3953 T14807 T14803 nmod mannose,form
R3954 T14723 T14717 conj abundance,response
R3955 T14808 T14807 punct -,mannose
R3956 T14809 T14803 amod glycosylated,form
R3957 T14810 T14789 punct .,explain
R3958 T14724 T14723 prep of,abundance
R3959 T14725 T14726 amod mature,protein
R396 T2615 T2613 auxpass been,established
R3960 T14812 T14813 det The,oligosaccharide
R3961 T14813 T14818 nsubjpass oligosaccharide,added
R3962 T14814 T14815 amod high,mannose
R3963 T14815 T14813 compound mannose,oligosaccharide
R3964 T14816 T14815 punct -,mannose
R3965 T14726 T14724 pobj protein,of
R3966 T14817 T14813 compound core,oligosaccharide
R3967 T14727 T14726 amod glycosylated,protein
R3968 T14819 T14818 auxpass is,added
R3969 T14820 T14818 prep in,added
R397 T2616 T2617 mark that,tetramerize
R3970 T14821 T14822 det the,ER
R3971 T14728 T14723 prep in,abundance
R3972 T14822 T14820 pobj ER,in
R3973 T14823 T14818 cc and,added
R3974 T14824 T14825 auxpass is,modified
R3975 T14729 T14730 compound mutant,animals
R3976 T14825 T14818 conj modified,added
R3977 T14826 T14825 advmod later,modified
R3978 T14730 T14728 pobj animals,in
R3979 T14827 T14825 cc and,modified
R398 T2617 T2613 ccomp tetramerize,established
R3980 T14828 T14825 conj elaborated,modified
R3981 T14829 T14828 prep in,elaborated
R3982 T14731 T14723 punct ", ",abundance
R3983 T14830 T14831 det the,apparatus
R3984 T14831 T14829 pobj apparatus,in
R3985 T14732 T14723 cc and,abundance
R3986 T14832 T14831 compound Golgi,apparatus
R3987 T14833 T14834 punct [,26
R3988 T14834 T14825 parataxis 26,modified
R3989 T14733 T14734 det the,transport
R399 T2618 T2617 nsubj aquaporins,tetramerize
R3990 T14835 T14834 punct ],26
R3991 T14836 T14818 punct .,added
R3992 T14734 T14723 conj transport,abundance
R3993 T14838 T14839 det The,increase
R3994 T14839 T14840 nsubj increase,reflect
R3995 T14735 T14734 prep of,transport
R3996 T14841 T14839 prep in,increase
R3997 T14842 T14843 det the,form
R3998 T14843 T14841 pobj form,in
R3999 T14736 T14737 det a,fraction
R400 T2619 T2617 punct ", ",tetramerize
R4000 T14737 T14735 pobj fraction,of
R4001 T14950 T14949 aux can,explain
R4002 T14844 T14845 amod high,mannose
R4003 T14845 T14843 nmod mannose,form
R4004 T14846 T14845 punct -,mannose
R4005 T14847 T14843 amod glycosylated,form
R4006 T14951 T14949 neg not,explain
R4007 T14848 T14843 prep of,form
R4008 T14849 T14850 compound AQP2,F204V
R4009 T14850 T14848 pobj F204V,of
R401 T2620 T2621 mark although,functional
R4010 T14952 T14953 det the,lack
R4011 T14851 T14850 punct -,F204V
R4012 T14852 T14840 aux may,reflect
R4013 T14853 T14840 advmod simply,reflect
R4014 T14953 T14949 dobj lack,explain
R4015 T14854 T14855 poss its,presence
R4016 T14954 T14953 prep of,lack
R4017 T14855 T14840 dobj presence,reflect
R4018 T14856 T14855 amod prolonged,presence
R4019 T14857 T14855 prep in,presence
R402 T2621 T2617 advcl functional,tetramerize
R4020 T14858 T14859 det the,ER
R4021 T14955 T14956 det any,protein
R4022 T14859 T14857 pobj ER,in
R4023 T14860 T14855 cc and,presence
R4024 T14861 T14855 conj exposure,presence
R4025 T14956 T14954 pobj protein,of
R4026 T14957 T14958 npadvmod ER,retained
R4027 T14862 T14861 prep to,exposure
R4028 T14863 T14864 compound oligosaccharyl,transferase
R4029 T14864 T14862 pobj transferase,to
R403 T2622 T2621 prep as,functional
R4030 T14958 T14956 amod retained,protein
R4031 T14865 T14840 punct .,reflect
R4032 T14867 T14868 mark While,explains
R4033 T14959 T14958 punct -,retained
R4034 T14868 T14873 advcl explains,is
R4035 T14960 T14956 compound mutant,protein
R4036 T14869 T14870 amod improper,localization
R4037 T14870 T14868 nsubj localization,explains
R4038 T14871 T14870 prep of,localization
R4039 T14961 T14949 punct .,explain
R404 T2623 T2624 det a,monomer
R4040 T14872 T14871 pobj AQP2,of
R4041 T14963 T14964 advmod Rather,suggests
R4042 T14874 T14875 det the,phenotype
R4043 T14875 T14868 dobj phenotype,explains
R4044 T14876 T14875 prep of,phenotype
R4045 T14877 T14878 amod homozygous,mice
R4046 T14878 T14876 pobj mice,of
R4047 T14965 T14964 punct ", ",suggests
R4048 T14879 T14878 compound mutant,mice
R4049 T14880 T14873 punct ", ",is
R405 T2624 T2622 pobj monomer,as
R4050 T14881 T14882 det the,lack
R4051 T14882 T14873 nsubj lack,is
R4052 T14883 T14882 amod complete,lack
R4053 T14884 T14882 prep of,lack
R4054 T14885 T14886 det a,phenotype
R4055 T14886 T14884 pobj phenotype,of
R4056 T14966 T14967 det the,phenotype
R4057 T14887 T14882 prep in,lack
R4058 T14888 T14889 amod heterozygous,mice
R4059 T14889 T14887 pobj mice,in
R406 T2625 T2617 punct ", ",tetramerize
R4060 T14967 T14964 nsubj phenotype,suggests
R4061 T14890 T14891 advmod more,difficult
R4062 T14891 T14873 acomp difficult,is
R4063 T14892 T14893 aux to,explain
R4064 T14968 T14967 prep of,phenotype
R4065 T14893 T14891 advcl explain,difficult
R4066 T14894 T14873 punct .,is
R4067 T14969 T14970 det the,animals
R4068 T14896 T14897 advmod Physiologically,have
R4069 T14970 T14968 pobj animals,of
R407 T2626 T2617 prep before,tetramerize
R4070 T14898 T14897 punct ", ",have
R4071 T14899 T14900 amod heterozygous,mice
R4072 T14900 T14897 nsubj mice,have
R4073 T14971 T14970 nmod Aqp2F204V,animals
R4074 T14901 T14902 det no,symptoms
R4075 T14902 T14897 dobj symptoms,have
R4076 T14903 T14904 punct (,see
R4077 T14972 T14971 punct /,Aqp2F204V
R4078 T14904 T14897 parataxis see,have
R4079 T14905 T14904 dobj Figure,see
R408 T2627 T2628 poss their,insertion
R4080 T14973 T14971 punct +,Aqp2F204V
R4081 T14906 T14905 nummod 1C,Figure
R4082 T14907 T14904 punct ),see
R4083 T14908 T14897 punct ", ",have
R4084 T14909 T14897 cc and,have
R4085 T14974 T14975 mark that,rescued
R4086 T14910 T14911 nsubj they,are
R4087 T14911 T14897 conj are,have
R4088 T14975 T14964 ccomp rescued,suggests
R4089 T14912 T14911 acomp indistinguishable,are
R409 T2628 T2626 pobj insertion,before
R4090 T14913 T14912 prep from,indistinguishable
R4091 T14914 T14915 amod wild,type
R4092 T14976 T14977 det the,protein
R4093 T14915 T14913 pobj type,from
R4094 T14916 T14911 prep on,are
R4095 T14917 T14916 pobj immunostaining,on
R4096 T14977 T14975 nsubjpass protein,rescued
R4097 T14918 T14917 prep of,immunostaining
R4098 T14919 T14918 pobj kidneys,of
R4099 T14920 T14921 punct (,Figure
R410 T2629 T2628 prep into,insertion
R4100 T14978 T14977 compound mutant,protein
R4101 T14921 T14911 parataxis Figure,are
R4102 T14922 T14921 nummod 5A,Figure
R4103 T14923 T14921 punct ),Figure
R4104 T14924 T14911 punct .,are
R4105 T14979 T14975 aux is,rescued
R4106 T14926 T14927 det The,presence
R4107 T14927 T14928 nsubj presence,explain
R4108 T14980 T14975 auxpass being,rescued
R4109 T14929 T14927 prep of,presence
R411 T2630 T2631 det the,membrane
R4110 T14930 T14931 nummod 50,%
R4111 T14931 T14929 pobj %,of
R4112 T14981 T14975 agent by,rescued
R4113 T14932 T14931 prep of,%
R4114 T14933 T14934 det the,amount
R4115 T14934 T14932 pobj amount,of
R4116 T14982 T14983 det the,protein
R4117 T14935 T14934 amod normal,amount
R4118 T14936 T14934 prep of,amount
R4119 T14937 T14938 amod wild,type
R412 T2631 T2629 pobj membrane,into
R4120 T14938 T14940 compound type,protein
R4121 T14983 T14981 pobj protein,by
R4122 T14939 T14938 punct -,type
R4123 T14940 T14936 pobj protein,of
R4124 T14984 T14985 amod wild,type
R4125 T14941 T14928 aux may,explain
R4126 T14942 T14943 det the,lack
R4127 T14943 T14928 dobj lack,explain
R4128 T14944 T14943 prep of,lack
R4129 T14985 T14983 compound type,protein
R413 T2632 T2631 compound plasma,membrane
R4130 T14945 T14944 pobj symptoms,of
R4131 T14946 T14928 punct ", ",explain
R4132 T14947 T14928 cc but,explain
R4133 T14986 T14985 punct -,type
R4134 T14948 T14949 nsubj it,explain
R4135 T14987 T14964 punct .,suggests
R4136 T14949 T14928 conj explain,explain
R4137 T14989 T14990 advmod Indeed,demonstrated
R4138 T14991 T14990 punct ", ",demonstrated
R4139 T14992 T14993 compound de,Mattia
R414 T2633 T2634 punct [,6
R4140 T14993 T14990 nsubj Mattia,demonstrated
R4141 T14994 T14995 advmod et,al.
R4142 T15056 T15055 pobj this,of
R4143 T15057 T15053 punct ", ",interact
R4144 T14995 T14993 advmod al.,Mattia
R4145 T15058 T15059 compound AQP2,F204V
R4146 T15059 T15053 nsubj F204V,interact
R4147 T15060 T15059 punct -,F204V
R4148 T14996 T14997 punct (,26
R4149 T15061 T15053 aux can,interact
R415 T2634 T2613 parataxis 6,established
R4150 T15062 T15053 prep with,interact
R4151 T14997 T14993 parataxis 26,Mattia
R4152 T15063 T15064 amod wild,type
R4153 T15064 T15066 compound type,AQP2
R4154 T15065 T15064 punct -,type
R4155 T15066 T15062 pobj AQP2,with
R4156 T15067 T15068 punct (,Figure
R4157 T15068 T15053 parataxis Figure,interact
R4158 T14998 T14997 punct ),26
R4159 T15069 T15068 nummod 5B,Figure
R416 T2635 T2634 nummod 4,6
R4160 T15070 T15068 punct ),Figure
R4161 T15071 T15053 punct ", ",interact
R4162 T15072 T15053 cc and,interact
R4163 T14999 T14990 aux have,demonstrated
R4164 T15073 T15074 advmod when,coexpressed
R4165 T15074 T15075 advcl coexpressed,reach
R4166 T15000 T15001 mark that,translocate
R4167 T15075 T15053 conj reach,interact
R4168 T15076 T15074 prep with,coexpressed
R4169 T15077 T15078 amod wild,type
R417 T2636 T2634 punct ",",6
R4170 T15001 T14990 advcl translocate,demonstrated
R4171 T15078 T15080 compound type,protein
R4172 T15079 T15078 punct -,type
R4173 T15080 T15076 pobj protein,with
R4174 T15002 T15003 nummod one,allele
R4175 T15081 T15075 punct ", ",reach
R4176 T15082 T15083 compound AQP2,F204V
R4177 T15083 T15075 nsubj F204V,reach
R4178 T15003 T15001 nsubj allele,translocate
R4179 T15084 T15083 punct -,F204V
R418 T2637 T2634 punct ],6
R4180 T15085 T15075 aux can,reach
R4181 T15086 T15087 det the,surface
R4182 T15004 T15003 amod recessive,allele
R4183 T15087 T15075 dobj surface,reach
R4184 T15088 T15087 compound cell,surface
R4185 T15089 T15090 punct (,panel
R4186 T15005 T15003 prep of,allele
R4187 T15090 T15075 parataxis panel,reach
R4188 T15091 T15090 nmod Figure,panel
R4189 T15092 T15091 nummod 5C,Figure
R419 T2638 T2613 punct .,established
R4190 T15093 T15094 npadvmod bottom,right
R4191 T15006 T15005 pobj Aqp2,of
R4192 T15094 T15090 amod right,panel
R4193 T15095 T15090 cc and,panel
R4194 T15096 T15090 conj 5D,panel
R4195 T15007 T15003 punct ", ",allele
R4196 T15097 T15090 punct ),panel
R4197 T15098 T15053 punct .,interact
R4198 T15008 T15003 appos P262L,allele
R4199 T15100 T15101 mark Although,demonstrated
R420 T2640 T2641 advmod Furthermore,targeted
R4200 T15101 T15105 advcl demonstrated,show
R4201 T15009 T15001 punct ", ",translocate
R4202 T15102 T15101 nsubjpass it,demonstrated
R4203 T15103 T15101 aux has,demonstrated
R4204 T15104 T15101 auxpass been,demonstrated
R4205 T15010 T15001 aux does,translocate
R4206 T15106 T15107 mark that,fails
R4207 T15107 T15101 ccomp fails,demonstrated
R4208 T15108 T15109 det a,allele
R4209 T15109 T15107 nsubj allele,fails
R421 T2641 T2648 ccomp targeted,routed
R4210 T15110 T15109 amod recessive,allele
R4211 T15011 T15001 neg not,translocate
R4212 T15111 T15109 punct (,allele
R4213 T15112 T15109 acl encoding,allele
R4214 T15113 T15114 compound AQP2,R187C
R4215 T15012 T15001 advmod properly,translocate
R4216 T15114 T15112 dobj R187C,encoding
R4217 T15115 T15114 punct -,R187C
R4218 T15116 T15109 punct ),allele
R4219 T15013 T15014 advmod when,expressed
R422 T2642 T2643 det these,proteins
R4220 T15117 T15109 prep of,allele
R4221 T15118 T15117 pobj NDI,of
R4222 T15014 T15001 advcl expressed,translocate
R4223 T15119 T15120 aux to,interact
R4224 T15015 T15014 prep by,expressed
R4225 T15120 T15107 xcomp interact,fails
R4226 T15121 T15120 prep with,interact
R4227 T15122 T15123 amod wild,type
R4228 T15016 T15015 pobj itself,by
R4229 T15123 T15125 compound type,AQP2
R423 T2643 T2641 nsubjpass proteins,targeted
R4230 T15124 T15123 punct -,type
R4231 T15125 T15121 pobj AQP2,with
R4232 T15017 T15014 prep in,expressed
R4233 T15126 T15127 punct [,6
R4234 T15127 T15101 parataxis 6,demonstrated
R4235 T15018 T15019 compound MDCK,cells
R4236 T15128 T15127 punct ],6
R4237 T15129 T15105 punct ", ",show
R4238 T15019 T15017 pobj cells,in
R4239 T15130 T15105 advmod here,show
R424 T2644 T2641 aux can,targeted
R4240 T15131 T15105 nsubj we,show
R4241 T15132 T15133 mark that,interact
R4242 T15133 T15105 ccomp interact,show
R4243 T15020 T15001 punct ", ",translocate
R4244 T15134 T15135 compound AQP2,F204V
R4245 T15135 T15133 nsubj F204V,interact
R4246 T15021 T15001 cc but,translocate
R4247 T15136 T15135 punct -,F204V
R4248 T15137 T15133 aux does,interact
R4249 T15138 T15133 prep with,interact
R425 T2645 T2641 advmod also,targeted
R4250 T15022 T15023 mark that,localizes
R4251 T15139 T15140 det the,protein
R4252 T15140 T15138 pobj protein,with
R4253 T15141 T15142 amod wild,type
R4254 T15023 T15001 conj localizes,translocate
R4255 T15142 T15140 compound type,protein
R4256 T15143 T15142 punct -,type
R4257 T15144 T15133 punct ", ",interact
R4258 T15145 T15146 advmod presumably,as
R4259 T15146 T15133 prep as,interact
R426 T2646 T2641 auxpass be,targeted
R4260 T15147 T15146 pobj part,as
R4261 T15024 T15023 prep in,localizes
R4262 T15148 T15147 prep of,part
R4263 T15149 T15148 pobj heterotetramers,of
R4264 T15025 T15026 det the,presence
R4265 T15150 T15133 punct ", ",interact
R4266 T15151 T15133 cc and,interact
R4267 T15152 T15133 conj represents,interact
R4268 T15153 T15154 det a,allele
R4269 T15026 T15024 pobj presence,in
R427 T2647 T2641 advmod differentially,targeted
R4270 T15154 T15152 dobj allele,represents
R4271 T15155 T15154 amod rescuable,allele
R4272 T15156 T15152 punct ", ",represents
R4273 T15027 T15026 prep of,presence
R4274 T15157 T15158 preconj both,vitro
R4275 T15158 T15152 advmod vitro,represents
R4276 T15028 T15029 amod wild,type
R4277 T15159 T15158 advmod in,vitro
R4278 T15160 T15158 cc and,vitro
R4279 T15029 T15031 compound type,protein
R428 T2649 T2641 prep to,targeted
R4280 T15030 T15029 punct -,type
R4281 T15031 T15027 pobj protein,of
R4282 T15161 T15162 advmod in,vivo
R4283 T15162 T15158 conj vivo,vitro
R4284 T15032 T15023 punct ", ",localizes
R4285 T15163 T15105 punct .,show
R4286 T15165 T15166 csubj Immunostaining,shows
R4287 T15033 T15023 nsubj it,localizes
R4288 T15034 T15023 advmod normally,localizes
R4289 T15167 T15168 det the,kidneys
R429 T2650 T2651 amod distinct,regions
R4290 T15168 T15165 dobj kidneys,Immunostaining
R4291 T15169 T15168 prep of,kidneys
R4292 T15170 T15171 amod homozygous,mice
R4293 T15035 T14990 punct .,demonstrated
R4294 T15171 T15169 pobj mice,of
R4295 T15172 T15173 compound Aqp2F204V,F204V
R4296 T15173 T15171 compound F204V,mice
R4297 T15037 T15038 det The,mechanism
R4298 T15174 T15173 punct /,F204V
R4299 T15175 T15176 mark that,mediate
R430 T2651 T2649 pobj regions,to
R4300 T15176 T15166 ccomp mediate,shows
R4301 T15177 T15178 det the,cells
R4302 T15038 T15040 nsubj mechanism,seems
R4303 T15178 T15176 nsubj cells,mediate
R4304 T15179 T15180 npadvmod mutant,expressing
R4305 T15039 T15038 amod same,mechanism
R4306 T15180 T15178 amod expressing,cells
R4307 T15181 T15180 punct -,expressing
R4308 T15182 T15183 amod collecting,duct
R4309 T15183 T15178 compound duct,cells
R431 T2652 T2651 prep of,regions
R4310 T15184 T15176 aux can,mediate
R4311 T15185 T15176 neg not,mediate
R4312 T15186 T15187 compound water,reabsorption
R4313 T15041 T15042 aux to,apply
R4314 T15187 T15176 dobj reabsorption,mediate
R4315 T15188 T15176 punct ", ",mediate
R4316 T15189 T15190 mark because,fails
R4317 T15190 T15176 advcl fails,mediate
R4318 T15191 T15190 nsubj it,fails
R4319 T15042 T15040 xcomp apply,seems
R432 T2653 T2654 det the,PM
R4320 T15192 T15193 aux to,insert
R4321 T15193 T15190 xcomp insert,fails
R4322 T15043 T15044 advmod in,vivo
R4323 T15194 T15193 prep into,insert
R4324 T15195 T15196 det the,membrane
R4325 T15196 T15194 pobj membrane,into
R4326 T15044 T15042 advmod vivo,apply
R4327 T15197 T15196 amod apical,membrane
R4328 T15198 T15196 compound plasma,membrane
R4329 T15199 T15193 prep in,insert
R433 T2654 T2652 pobj PM,of
R4330 T15045 T15042 prep with,apply
R4331 T15200 T15199 pobj response,in
R4332 T15046 T15047 nmod Aqp2F204V,mice
R4333 T15047 T15045 pobj mice,with
R4334 T15048 T15046 punct /,Aqp2F204V
R4335 T15201 T15200 prep to,response
R4336 T15049 T15046 punct +,Aqp2F204V
R4337 T15050 T15040 punct .,seems
R4338 T15202 T15201 pobj dDAVP,to
R4339 T15203 T15166 punct .,shows
R434 T2655 T2648 punct ;,routed
R4340 T15052 T15053 prep In,interact
R4341 T15205 T15206 nsubj This,is
R4342 T15054 T15052 pobj support,In
R4343 T15207 T15208 det this,proof
R4344 T15208 T15206 attr proof,is
R4345 T15209 T15208 amod first,proof
R4346 T15055 T15054 prep of,support
R4347 T15210 T15211 advmod in,vivo
R4348 T15211 T15208 amod vivo,proof
R4349 T15212 T15208 prep of,proof
R435 T2656 T2648 prep for,routed
R4350 T15267 T15268 advmod Thus,proposed
R4351 T15213 T15214 det a,hypothesis
R4352 T15214 T15212 pobj hypothesis,of
R4353 T15215 T15216 amod long,standing
R4354 T15216 T15214 amod standing,hypothesis
R4355 T15217 T15216 punct -,standing
R4356 T15269 T15268 punct ", ",proposed
R4357 T15218 T15219 dep that,comes
R4358 T15219 T15214 relcl comes,hypothesis
R4359 T15220 T15219 prep from,comes
R436 T2657 T2656 pobj example,for
R4360 T15221 T15222 advmod in,vitro
R4361 T15222 T15223 amod vitro,studies
R4362 T15223 T15220 pobj studies,from
R4363 T15224 T15223 prep with,studies
R4364 T15270 T15268 nsubjpass misfolding,proposed
R4365 T15225 T15226 amod recessive,mutations
R4366 T15226 T15224 pobj mutations,with
R4367 T15227 T15226 compound Aqp2,mutations
R4368 T15228 T15206 punct .,is
R4369 T15271 T15270 punct ", ",misfolding
R437 T2658 T2648 punct ", ",routed
R4370 T15230 T15231 nsubj Transfection,shows
R4371 T15272 T15270 conj retention,misfolding
R4372 T15232 T15230 prep into,Transfection
R4373 T15233 T15234 compound MDCK,cells
R4374 T15273 T15272 prep in,retention
R4375 T15234 T15232 pobj cells,into
R4376 T15235 T15230 prep of,Transfection
R4377 T15236 T15235 pobj any,of
R4378 T15274 T15275 det the,ER
R4379 T15237 T15236 prep of,any
R438 T2659 T2648 nsubjpass AQP2,routed
R4380 T15238 T15239 amod several,mutations
R4381 T15239 T15237 pobj mutations,of
R4382 T15275 T15273 pobj ER,in
R4383 T15240 T15239 compound Aqp2,mutations
R4384 T15241 T15239 acl corresponding,mutations
R4385 T15276 T15272 punct ", ",retention
R4386 T15242 T15241 prep to,corresponding
R4387 T15243 T15244 amod recessive,alleles
R4388 T15244 T15242 pobj alleles,to
R4389 T15245 T15244 amod human,alleles
R439 T2660 T2648 auxpass is,routed
R4390 T15277 T15272 cc and,retention
R4391 T15246 T15247 amod abnormal,localization
R4392 T15247 T15231 dobj localization,shows
R4393 T15278 T15272 conj failure,retention
R4394 T15248 T15247 amod subcellular,localization
R4395 T15249 T15250 punct [,25
R4396 T15279 T15280 aux to,translocate
R4397 T15250 T15247 parataxis 25,localization
R4398 T15251 T15250 punct ],25
R4399 T15252 T15247 punct ", ",localization
R440 T2661 T2648 prep to,routed
R4400 T15280 T15278 acl translocate,failure
R4401 T15253 T15254 punct [,27
R4402 T15254 T15247 parataxis 27,localization
R4403 T15255 T15254 punct ],27
R4404 T15281 T15280 prep in,translocate
R4405 T15256 T15247 cc and,localization
R4406 T15257 T15247 conj failure,localization
R4407 T15258 T15259 aux to,translocate
R4408 T15282 T15281 pobj response,in
R4409 T15259 T15257 acl translocate,failure
R441 T2662 T2663 det the,membrane
R4410 T15260 T15259 advmod appropriately,translocate
R4411 T15261 T15259 prep to,translocate
R4412 T15283 T15282 prep to,response
R4413 T15262 T15263 det the,membrane
R4414 T15263 T15261 pobj membrane,to
R4415 T15264 T15263 compound plasma,membrane
R4416 T15265 T15231 punct .,shows
R4417 T15284 T15283 pobj dDAVP,to
R4418 T15285 T15268 auxpass were,proposed
R4419 T15286 T15268 prep as,proposed
R442 T2663 T2661 pobj membrane,to
R4420 T15287 T15288 det the,mechanism
R4421 T15288 T15286 pobj mechanism,as
R4422 T15289 T15288 prep for,mechanism
R4423 T15290 T15291 amod autosomal,NDI
R4424 T15291 T15289 pobj NDI,for
R4425 T15292 T15291 amod recessive,NDI
R4426 T15293 T15268 punct .,proposed
R4427 T15295 T15296 advmod Here,prove
R4428 T15297 T15296 nsubj we,prove
R4429 T15298 T15296 preconj not,prove
R443 T2664 T2663 amod apical,membrane
R4430 T15299 T15298 advmod only,not
R4431 T15300 T15301 det this,hypothesis
R4432 T15301 T15296 dobj hypothesis,prove
R4433 T15302 T15296 cc but,prove
R4434 T15303 T15302 advmod also,but
R4435 T15304 T15296 conj establish,prove
R4436 T15305 T15306 det a,model
R4437 T15306 T15304 dobj model,establish
R4438 T15307 T15306 amod useful,model
R4439 T15308 T15306 prep for,model
R444 T2665 T2663 prep of,membrane
R4440 T15309 T15310 amod human,NDI
R4441 T15310 T15308 pobj NDI,for
R4442 T15311 T15296 punct .,prove
R4443 T15313 T15314 det This,model
R4444 T15314 T15316 nsubj model,provides
R4445 T15315 T15314 compound mouse,model
R4446 T15317 T15314 prep of,model
R4447 T15318 T15317 pobj NDI,of
R4448 T15319 T15314 prep based,model
R4449 T15320 T15319 prep on,based
R445 T2666 T2665 pobj cells,of
R4450 T15321 T15322 det an,allele
R4451 T15322 T15320 pobj allele,on
R4452 T15323 T15322 compound Aqp2,allele
R4453 T15324 T15325 dep that,rescued
R4454 T15325 T15322 relcl rescued,allele
R4455 T15326 T15325 aux can,rescued
R4456 T15327 T15325 auxpass be,rescued
R4457 T15328 T15329 det the,opportunity
R4458 T15329 T15316 dobj opportunity,provides
R4459 T15330 T15331 aux to,test
R446 T2667 T2666 acl surrounding,cells
R4460 T15331 T15329 acl test,opportunity
R4461 T15332 T15331 dobj therapies,test
R4462 T15333 T15332 punct ", ",therapies
R4463 T15334 T15332 prep including,therapies
R4464 T15335 T15336 compound gene,therapy
R4465 T15336 T15334 pobj therapy,including
R4466 T15337 T15332 punct ", ",therapies
R4467 T15338 T15339 dep that,promote
R4468 T15339 T15332 relcl promote,therapies
R4469 T15340 T15339 aux may,promote
R447 T2668 T2669 det the,duct
R4470 T15341 T15342 amod proper,localization
R4471 T15342 T15339 dobj localization,promote
R4472 T15343 T15342 amod subcellular,localization
R4473 T15344 T15316 punct .,provides
R4474 T15508 T15507 prep of,Generation
R4475 T15509 T15510 compound ENU,mice
R4476 T15510 T15508 pobj mice,of
R4477 T15511 T15507 cc and,Generation
R4478 T15512 T15507 conj housing,Generation
R4479 T15513 T15512 punct .,housing
R448 T2669 T2667 dobj duct,surrounding
R4480 T15515 T15516 nmod ENU,mice
R4481 T15516 T15521 nsubjpass mice,generated
R4482 T15517 T15516 amod mutagenized,mice
R4483 T15518 T15516 nmod C57BL,mice
R4484 T15519 T15518 punct /,C57BL
R4485 T15520 T15518 nummod 6,C57BL
R4486 T15522 T15521 auxpass were,generated
R4487 T15523 T15524 mark as,described
R4488 T15524 T15521 advcl described,generated
R4489 T15525 T15526 punct [,19
R449 T2670 T2669 amod collecting,duct
R4490 T15526 T15521 parataxis 19,generated
R4491 T15527 T15526 punct ],19
R4492 T15528 T15521 punct .,generated
R4493 T15530 T15531 nsubjpass Mice,maintained
R4494 T15532 T15531 auxpass were,maintained
R4495 T15533 T15531 prep by,maintained
R4496 T15534 T15533 pcomp backcrossing,by
R4497 T15535 T15536 amod affected,animals
R4498 T15536 T15534 dobj animals,backcrossing
R4499 T15537 T15534 prep to,backcrossing
R450 T2671 T2648 punct ", ",routed
R4500 T15538 T15537 pobj C57BL,to
R4501 T15539 T15538 punct /,C57BL
R4502 T15540 T15538 nummod 6,C57BL
R4503 T15541 T15531 cc and,maintained
R4504 T15542 T15531 conj housed,maintained
R4505 T15543 T15542 prep in,housed
R4506 T15544 T15545 det the,Institute
R4507 T15545 T15543 pobj Institute,in
R4508 T15546 T15545 compound Genomics,Institute
R4509 T15547 T15545 prep of,Institute
R451 T2672 T2673 mark whereas,inserted
R4510 T15548 T15549 det the,facility
R4511 T15549 T15547 pobj facility,of
R4512 T15550 T15551 compound Novartis,Foundation
R4513 T15551 T15549 compound Foundation,facility
R4514 T15552 T15551 compound Research,Foundation
R4515 T15553 T15554 compound Specific,Pathogen
R4516 T15554 T15555 compound Pathogen,Free
R4517 T15555 T15549 compound Free,facility
R4518 T15556 T15549 compound animal,facility
R4519 T15557 T15558 punct (,Jolla
R452 T2673 T2648 advcl inserted,routed
R4520 T15558 T15549 parataxis Jolla,facility
R4521 T15559 T15558 compound La,Jolla
R4522 T15560 T15558 punct ", ",Jolla
R4523 T15561 T15558 npadvmod California,Jolla
R4524 T15562 T15558 punct ", ",Jolla
R4525 T15563 T15564 compound United,States
R4526 T15564 T15558 npadvmod States,Jolla
R4527 T15565 T15558 punct ),Jolla
R4528 T15566 T15531 punct .,maintained
R4529 T15568 T15569 det All,procedures
R453 T2674 T2675 amod other,aquaporins
R4530 T15569 T15570 nsubjpass procedures,approved
R4531 T15571 T15570 auxpass were,approved
R4532 T15572 T15570 agent by,approved
R4533 T15573 T15574 det the,Institute
R4534 T15574 T15572 pobj Institute,by
R4535 T15575 T15574 compound Genomics,Institute
R4536 T15576 T15574 prep of,Institute
R4537 T15577 T15578 det the,Committee
R4538 T15578 T15576 pobj Committee,of
R4539 T15579 T15580 nmod Novartis,Foundation
R454 T2675 T2673 nsubjpass aquaporins,inserted
R4540 T15580 T15578 nmod Foundation,Committee
R4541 T15581 T15580 nmod Research,Foundation
R4542 T15582 T15583 nmod Institutional,Animal
R4543 T15583 T15578 nmod Animal,Committee
R4544 T15584 T15583 appos Care,Animal
R4545 T15585 T15584 cc and,Care
R4546 T15586 T15584 conj Use,Care
R4547 T15587 T15570 punct .,approved
R4548 T15901 T15900 punct .,Constructs
R4549 T15903 T15904 det The,sequence
R455 T2676 T2675 punct (,aquaporins
R4550 T15904 T15907 nsubjpass sequence,digested
R4551 T15905 T15904 amod complete,sequence
R4552 T15906 T15904 compound coding,sequence
R4553 T15908 T15904 prep of,sequence
R4554 T15909 T15910 compound mouse,AQP2
R4555 T15910 T15908 pobj AQP2,of
R4556 T15911 T15904 prep from,sequence
R4557 T15912 T15913 det an,clone
R4558 T15913 T15911 pobj clone,from
R4559 T15914 T15913 compound IMAGE,clone
R456 T2677 T2675 appos AQP3,aquaporins
R4560 T15915 T15907 auxpass was,digested
R4561 T15916 T15907 prep from,digested
R4562 T15917 T15918 det the,plasmid
R4563 T15918 T15916 pobj plasmid,from
R4564 T15919 T15918 compound pCMV⋅SPORT6,plasmid
R4565 T15920 T15907 prep with,digested
R4566 T15921 T15920 pobj EcoRI,with
R4567 T15922 T15921 cc and,EcoRI
R4568 T15923 T15921 conj NotI,EcoRI
R4569 T15924 T15907 cc and,digested
R457 T2678 T2677 cc or,AQP3
R4570 T15925 T15907 conj ligated,digested
R4571 T15926 T15925 prep into,ligated
R4572 T15927 T15926 pobj pcDNA3.1,into
R4573 T15928 T15929 punct (,Invitrogen
R4574 T15929 T15927 parataxis Invitrogen,pcDNA3.1
R4575 T15930 T15929 punct ", ",Invitrogen
R4576 T15931 T15929 npadvmod Carlsbad,Invitrogen
R4577 T15932 T15929 punct ", ",Invitrogen
R4578 T15933 T15929 npadvmod California,Invitrogen
R4579 T15934 T15929 punct ", ",Invitrogen
R458 T2679 T2677 conj 4,AQP3
R4580 T15935 T15936 compound United,States
R4581 T15936 T15929 npadvmod States,Invitrogen
R4582 T15937 T15929 punct ),Invitrogen
R4583 T15938 T15907 punct .,digested
R4584 T15940 T15941 det The,mutation
R4585 T15941 T15943 nsubjpass mutation,introduced
R4586 T15942 T15941 compound F204V,mutation
R4587 T15944 T15943 auxpass was,introduced
R4588 T15945 T15943 prep by,introduced
R4589 T15946 T15947 npadvmod site,directed
R459 T2680 T2673 punct ),inserted
R4590 T15947 T15949 amod directed,mutagenesis
R4591 T15948 T15947 punct -,directed
R4592 T15949 T15945 pobj mutagenesis,by
R4593 T15950 T15951 punct (,Stratagene
R4594 T15951 T15949 parataxis Stratagene,mutagenesis
R4595 T15952 T15951 punct ", ",Stratagene
R4596 T15953 T15954 compound La,Jolla
R4597 T15954 T15951 npadvmod Jolla,Stratagene
R4598 T15955 T15951 punct ", ",Stratagene
R4599 T15956 T15951 npadvmod California,Stratagene
R460 T2681 T2673 auxpass are,inserted
R4600 T15957 T15951 punct ", ",Stratagene
R4601 T15958 T15959 compound United,States
R4602 T15959 T15951 npadvmod States,Stratagene
R4603 T15960 T15951 punct ),Stratagene
R4604 T15961 T15943 punct ", ",introduced
R4605 T15962 T15943 advcl using,introduced
R4606 T15963 T15964 det the,oligonucleotide
R4607 T15964 T15962 dobj oligonucleotide,using
R4608 T15965 T15964 compound sense,oligonucleotide
R4609 T15966 T15967 nummod 5,GATGATCACTGGGTCGTCTGGATCGGACCCC
R461 T2682 T2673 prep into,inserted
R4610 T15967 T15964 appos GATGATCACTGGGTCGTCTGGATCGGACCCC,oligonucleotide
R4611 T15968 T15966 punct ′,5
R4612 T15969 T15967 punct -,GATGATCACTGGGTCGTCTGGATCGGACCCC
R4613 T15970 T15967 punct -,GATGATCACTGGGTCGTCTGGATCGGACCCC
R4614 T15971 T15967 nummod 3,GATGATCACTGGGTCGTCTGGATCGGACCCC
R4615 T15972 T15967 punct ′,GATGATCACTGGGTCGTCTGGATCGGACCCC
R4616 T15973 T15964 punct ", ",oligonucleotide
R4617 T15974 T15964 cc and,oligonucleotide
R4618 T15975 T15976 amod antisense,oligonucleotide
R4619 T15976 T15964 conj oligonucleotide,oligonucleotide
R462 T2683 T2684 det the,face
R4620 T15977 T15978 nummod 5,GGGGTCCGATCCAGACGACCCAGTGATCATC
R4621 T15978 T15976 appos GGGGTCCGATCCAGACGACCCAGTGATCATC,oligonucleotide
R4622 T15979 T15977 punct ′,5
R4623 T15980 T15978 punct -,GGGGTCCGATCCAGACGACCCAGTGATCATC
R4624 T15981 T15978 punct -,GGGGTCCGATCCAGACGACCCAGTGATCATC
R4625 T15982 T15978 nummod 3,GGGGTCCGATCCAGACGACCCAGTGATCATC
R4626 T15983 T15978 punct ′,GGGGTCCGATCCAGACGACCCAGTGATCATC
R4627 T15984 T15943 punct .,introduced
R4628 T15986 T15987 aux To,generate
R4629 T15987 T15988 advcl generate,used
R463 T2684 T2682 pobj face,into
R4630 T15989 T15990 compound GFP,fusions
R4631 T15990 T15987 dobj fusions,generate
R4632 T15991 T15990 prep of,fusions
R4633 T15992 T15991 pobj AQP2,of
R4634 T15993 T15988 punct ", ",used
R4635 T15994 T15995 det the,construct
R4636 T15995 T15988 nsubjpass construct,used
R4637 T15996 T15995 compound pCMV⋅SPORT6,construct
R4638 T15997 T15995 compound AQP2,construct
R4639 T15998 T15988 auxpass was,used
R464 T2685 T2684 amod basolateral,face
R4640 T15999 T15988 prep in,used
R4641 T16000 T16001 det a,reaction
R4642 T16001 T15999 pobj reaction,in
R4643 T16002 T16001 compound PCR,reaction
R4644 T16003 T16001 prep with,reaction
R4645 T16004 T16005 det the,Sp6
R4646 T16005 T16003 pobj Sp6,with
R4647 T16006 T16005 compound primers,Sp6
R4648 T16007 T16005 cc and,Sp6
R4649 T16008 T16009 nummod 5,GACTGGATCCCGGCCTTGCTGCCGCGCGGCAG
R465 T2686 T2648 punct .,routed
R4650 T16009 T16005 conj GACTGGATCCCGGCCTTGCTGCCGCGCGGCAG,Sp6
R4651 T16010 T16008 punct ′,5
R4652 T16011 T16009 punct -,GACTGGATCCCGGCCTTGCTGCCGCGCGGCAG
R4653 T16012 T16009 punct -,GACTGGATCCCGGCCTTGCTGCCGCGCGGCAG
R4654 T16013 T16009 nummod 3,GACTGGATCCCGGCCTTGCTGCCGCGCGGCAG
R4655 T16014 T16009 punct ′,GACTGGATCCCGGCCTTGCTGCCGCGCGGCAG
R4656 T16015 T16016 aux to,remove
R4657 T16016 T15988 advcl remove,used
R4658 T16017 T16018 det the,codon
R4659 T16018 T16016 dobj codon,remove
R466 T2688 T2689 prep Unlike,inserted
R4660 T16019 T16018 compound stop,codon
R4661 T16020 T16018 prep of,codon
R4662 T16021 T16020 pobj AQP2,of
R4663 T16022 T15988 punct .,used
R4664 T16024 T16025 det The,product
R4665 T16025 T16026 nsubjpass product,digested
R4666 T16027 T16026 auxpass was,digested
R4667 T16028 T16026 prep with,digested
R4668 T16029 T16028 pobj KpnI,with
R4669 T16030 T16029 cc and,KpnI
R467 T2690 T2691 det all,members
R4670 T16031 T16029 conj BamHI,KpnI
R4671 T16032 T16026 cc and,digested
R4672 T16033 T16026 conj ligated,digested
R4673 T16034 T16033 prep into,ligated
R4674 T16035 T16036 compound pEGFP,N2
R4675 T16036 T16034 pobj N2,into
R4676 T16037 T16036 punct -,N2
R4677 T16038 T16039 punct (,Biosciences
R4678 T16039 T16036 parataxis Biosciences,N2
R4679 T16040 T16039 compound BD,Biosciences
R468 T2691 T2688 pobj members,Unlike
R4680 T16041 T16039 punct ", ",Biosciences
R4681 T16042 T16043 compound San,Diego
R4682 T16043 T16039 npadvmod Diego,Biosciences
R4683 T16044 T16039 punct ", ",Biosciences
R4684 T16045 T16039 npadvmod California,Biosciences
R4685 T16046 T16039 punct ", ",Biosciences
R4686 T16047 T16048 compound United,States
R4687 T16048 T16039 npadvmod States,Biosciences
R4688 T16049 T16039 punct ),Biosciences
R4689 T16050 T16026 punct .,digested
R469 T2692 T2691 amod other,members
R4690 T16052 T16053 det The,mutation
R4691 T16053 T16055 nsubjpass mutation,introduced
R4692 T16054 T16053 compound F204V,mutation
R4693 T16056 T16055 auxpass was,introduced
R4694 T16057 T16055 advcl using,introduced
R4695 T16058 T16059 det the,oligonucleotides
R4696 T16059 T16057 dobj oligonucleotides,using
R4697 T16060 T16059 amod same,oligonucleotides
R4698 T16061 T16059 amod mutagenic,oligonucleotides
R4699 T16062 T16055 punct .,introduced
R470 T2693 T2691 compound family,members
R4700 T16399 T16400 compound Cell,culture
R4701 T16401 T16400 cc and,culture
R4702 T16402 T16400 conj generation,culture
R4703 T16403 T16400 prep of,culture
R4704 T16404 T16405 amod stable,lines
R4705 T16405 T16403 pobj lines,of
R4706 T16406 T16405 compound cell,lines
R4707 T16407 T16400 punct .,culture
R4708 T16409 T16410 compound MDCK,cells
R4709 T16410 T16411 nsubjpass cells,cultured
R471 T2694 T2689 punct ", ",inserted
R4710 T16412 T16413 punct (,ATCC
R4711 T16413 T16410 parataxis ATCC,cells
R4712 T16414 T16413 dep CCL,ATCC
R4713 T16415 T16414 punct -,CCL
R4714 T16416 T16414 nummod 34,CCL
R4715 T16417 T16413 punct ;,ATCC
R4716 T16418 T16413 punct ", ",ATCC
R4717 T16419 T16413 npadvmod Manassas,ATCC
R4718 T16420 T16413 punct ", ",ATCC
R4719 T16421 T16413 npadvmod Virginia,ATCC
R472 T2695 T2689 nsubjpass AQP2,inserted
R4720 T16422 T16413 punct ", ",ATCC
R4721 T16423 T16424 compound United,States
R4722 T16424 T16413 npadvmod States,ATCC
R4723 T16425 T16413 punct ),ATCC
R4724 T16426 T16411 auxpass were,cultured
R4725 T16427 T16411 prep in,cultured
R4726 T16428 T16427 pobj DMEM,in
R4727 T16429 T16430 punct (,Aldrich
R4728 T16430 T16428 parataxis Aldrich,DMEM
R4729 T16431 T16430 compound Sigma,Aldrich
R473 T2696 T2689 auxpass is,inserted
R4730 T16432 T16430 punct -,Aldrich
R4731 T16433 T16430 punct ", ",Aldrich
R4732 T16434 T16435 compound St.,Louis
R4733 T16435 T16430 npadvmod Louis,Aldrich
R4734 T16436 T16430 punct ", ",Aldrich
R4735 T16437 T16430 npadvmod Missouri,Aldrich
R4736 T16438 T16430 punct ", ",Aldrich
R4737 T16439 T16440 compound United,States
R4738 T16440 T16430 npadvmod States,Aldrich
R4739 T16441 T16430 punct ),Aldrich
R474 T2697 T2689 neg not,inserted
R4740 T16442 T16411 dep supplemented,cultured
R4741 T16443 T16442 prep with,supplemented
R4742 T16444 T16445 nummod 10,%
R4743 T16445 T16446 compound %,FBS
R4744 T16446 T16443 pobj FBS,with
R4745 T16447 T16448 punct (,Aldrich
R4746 T16448 T16446 parataxis Aldrich,FBS
R4747 T16449 T16448 compound Sigma,Aldrich
R4748 T16450 T16448 punct -,Aldrich
R4749 T16451 T16448 punct ),Aldrich
R475 T2698 T2689 advmod constitutively,inserted
R4750 T16452 T16446 punct ", ",FBS
R4751 T16453 T16454 nummod 100,U
R4752 T16454 T16446 conj U,FBS
R4753 T16455 T16456 punct /,ml
R4754 T16456 T16454 prep ml,U
R4755 T16457 T16454 prep of,U
R4756 T16458 T16457 pobj penicillin,of
R4757 T16459 T16454 punct ", ",U
R4758 T16460 T16454 cc and,U
R4759 T16461 T16462 nummod 100,μg
R476 T2699 T2689 prep into,inserted
R4760 T16462 T16454 conj μg,U
R4761 T16463 T16464 punct /,ml
R4762 T16464 T16462 prep ml,μg
R4763 T16465 T16462 prep of,μg
R4764 T16466 T16465 pobj streptomycin,of
R4765 T16467 T16446 prep at,FBS
R4766 T16468 T16469 nummod 37,°C
R4767 T16469 T16467 pobj °C,at
R4768 T16470 T16446 prep in,FBS
R4769 T16471 T16472 nummod 5,%
R477 T2700 T2701 det the,membrane
R4770 T16472 T16473 compound %,CO2
R4771 T16473 T16470 pobj CO2,in
R4772 T16474 T16411 punct .,cultured
R4773 T16476 T16477 aux To,generate
R4774 T16477 T16478 advcl generate,transfected
R4775 T16479 T16480 amod stable,lines
R4776 T16480 T16477 dobj lines,generate
R4777 T16481 T16480 compound MDCK,lines
R4778 T16482 T16480 compound cell,lines
R4779 T16483 T16478 punct ", ",transfected
R478 T2701 T2699 pobj membrane,into
R4780 T16484 T16478 nsubjpass cells,transfected
R4781 T16485 T16478 auxpass were,transfected
R4782 T16486 T16478 advcl using,transfected
R4783 T16487 T16486 dobj Lipofectamine,using
R4784 T16488 T16487 nummod 2000,Lipofectamine
R4785 T16489 T16490 punct (,Invitrogen
R4786 T16490 T16487 parataxis Invitrogen,Lipofectamine
R4787 T16491 T16490 punct ),Invitrogen
R4788 T16492 T16487 cc and,Lipofectamine
R4789 T16493 T16494 det the,constructs
R479 T2702 T2701 compound plasma,membrane
R4790 T16494 T16487 conj constructs,Lipofectamine
R4791 T16495 T16494 compound pcDNA3.1,constructs
R4792 T16496 T16494 compound expression,constructs
R4793 T16497 T16494 punct (,constructs
R4794 T16498 T16494 acl containing,constructs
R4795 T16499 T16500 amod wild,type
R4796 T16500 T16502 compound type,AQP2
R4797 T16501 T16500 punct -,type
R4798 T16502 T16498 dobj AQP2,containing
R4799 T16503 T16502 punct ", ",AQP2
R480 T2703 T2689 punct .,inserted
R4800 T16504 T16505 compound AQP2,F204V
R4801 T16505 T16502 conj F204V,AQP2
R4802 T16506 T16505 punct -,F204V
R4803 T16507 T16505 punct ", ",F204V
R4804 T16508 T16505 cc or,F204V
R4805 T16509 T16510 det no,insert
R4806 T16510 T16505 conj insert,F204V
R4807 T16511 T16494 punct ),constructs
R4808 T16512 T16478 cc and,transfected
R4809 T16513 T16478 conj selected,transfected
R481 T2705 T2706 prep Under,resides
R4810 T16514 T16513 prep with,selected
R4811 T16515 T16516 nummod 900,μg
R4812 T16516 T16517 nmod μg,G418
R4813 T16517 T16514 pobj G418,with
R4814 T16518 T16519 punct /,ml
R4815 T16519 T16516 prep ml,μg
R4816 T16520 T16521 punct (,Aldrich
R4817 T16521 T16517 parataxis Aldrich,G418
R4818 T16522 T16521 compound Sigma,Aldrich
R4819 T16523 T16521 punct -,Aldrich
R482 T2706 T2712 ccomp resides,translocates
R4820 T16524 T16521 punct ),Aldrich
R4821 T16525 T16478 punct .,transfected
R4822 T16527 T16528 amod Individual,colonies
R4823 T16528 T16529 nsubjpass colonies,expanded
R4824 T16530 T16529 auxpass were,expanded
R4825 T16531 T16532 nummod 14,d
R4826 T16532 T16533 npadvmod d,later
R4827 T16533 T16529 advmod later,expanded
R4828 T16534 T16529 punct .,expanded
R4829 T16536 T16537 prep For,added
R483 T2707 T2708 amod basal,conditions
R4830 T16538 T16539 det the,duration
R4831 T16539 T16536 pobj duration,For
R4832 T16540 T16539 prep of,duration
R4833 T16541 T16542 det these,experiments
R4834 T16542 T16540 pobj experiments,of
R4835 T16543 T16537 punct ", ",added
R4836 T16544 T16545 det the,antibiotic
R4837 T16545 T16537 nsubjpass antibiotic,added
R4838 T16546 T16537 auxpass was,added
R4839 T16547 T16537 advmod continually,added
R484 T2708 T2705 pobj conditions,Under
R4840 T16548 T16537 prep to,added
R4841 T16549 T16550 det the,media
R4842 T16550 T16548 pobj media,to
R4843 T16551 T16537 punct .,added
R4844 T16553 T16554 amod Transient,transfections
R4845 T16554 T16556 nsubjpass transfections,carried
R4846 T16555 T16554 compound GFP,transfections
R4847 T16557 T16556 auxpass were,carried
R4848 T16558 T16556 prt out,carried
R4849 T16559 T16556 prep in,carried
R485 T2709 T2706 punct ", ",resides
R4850 T16560 T16561 amod subconfluent,lines
R4851 T16561 T16559 pobj lines,in
R4852 T16562 T16561 amod stable,lines
R4853 T16563 T16561 compound cells,lines
R4854 T16564 T16556 advmod also,carried
R4855 T16565 T16556 advcl using,carried
R4856 T16566 T16565 dobj Lipofectamine,using
R4857 T16567 T16566 nummod 2000,Lipofectamine
R4858 T16568 T16556 punct .,carried
R4859 T16677 T16676 prep of,Sequencing
R486 T2710 T2711 det the,protein
R4860 T16678 T16677 pobj Aqp2,of
R4861 T16679 T16676 cc and,Sequencing
R4862 T16680 T16676 conj genotyping,Sequencing
R4863 T16681 T16680 prep of,genotyping
R4864 T16682 T16681 pobj mice,of
R4865 T16683 T16680 punct .,genotyping
R4866 T16685 T16686 det All,exons
R4867 T16686 T16687 nsubjpass exons,amplified
R4868 T16688 T16686 prep of,exons
R4869 T16689 T16688 pobj Aqp2,of
R487 T2711 T2706 nsubj protein,resides
R4870 T16690 T16687 auxpass were,amplified
R4871 T16691 T16687 prep from,amplified
R4872 T16692 T16693 nmod mouse,DNA
R4873 T16693 T16691 pobj DNA,from
R4874 T16694 T16693 amod genomic,DNA
R4875 T16695 T16687 cc and,amplified
R4876 T16696 T16687 conj sequenced,amplified
R4877 T16697 T16687 punct .,amplified
R4878 T16699 T16700 prep For,amplified
R4879 T16701 T16699 pobj genotyping,For
R488 T2713 T2706 prep in,resides
R4880 T16702 T16700 punct ", ",amplified
R4881 T16703 T16700 nsubjpass exon,amplified
R4882 T16704 T16703 nummod 4,exon
R4883 T16705 T16700 auxpass was,amplified
R4884 T16706 T16700 advcl using,amplified
R4885 T16707 T16708 det the,primers
R4886 T16708 T16706 dobj primers,using
R4887 T16709 T16710 nummod 5,TCAGAACTTGCCCACTAGCC
R4888 T16710 T16708 appos TCAGAACTTGCCCACTAGCC,primers
R4889 T16711 T16709 punct ′,5
R489 T2714 T2715 amod subapical,vesicles
R4890 T16712 T16710 punct -,TCAGAACTTGCCCACTAGCC
R4891 T16713 T16710 punct -,TCAGAACTTGCCCACTAGCC
R4892 T16714 T16710 nummod 3,TCAGAACTTGCCCACTAGCC
R4893 T16715 T16710 punct ′,TCAGAACTTGCCCACTAGCC
R4894 T16716 T16710 cc and,TCAGAACTTGCCCACTAGCC
R4895 T16717 T16718 nummod 5,TGTAGAGGAGGGAACCGATG
R4896 T16718 T16710 conj TGTAGAGGAGGGAACCGATG,TCAGAACTTGCCCACTAGCC
R4897 T16719 T16717 punct ′,5
R4898 T16720 T16718 punct -,TGTAGAGGAGGGAACCGATG
R4899 T16721 T16718 punct -,TGTAGAGGAGGGAACCGATG
R490 T2715 T2713 pobj vesicles,in
R4900 T16722 T16718 nummod 3,TGTAGAGGAGGGAACCGATG
R4901 T16723 T16718 punct ′,TGTAGAGGAGGGAACCGATG
R4902 T16724 T16700 punct .,amplified
R4903 T16959 T16960 compound Urine,measurements
R4904 T16961 T16960 punct .,measurements
R4905 T16963 T16964 amod Total,output
R4906 T16964 T16966 nsubjpass output,measured
R4907 T16965 T16964 compound urine,output
R4908 T16967 T16966 auxpass was,measured
R4909 T16968 T16966 prep by,measured
R491 T2716 T2715 amod intracellular,vesicles
R4910 T16969 T16970 advmod separately,housing
R4911 T16970 T16968 pcomp housing,by
R4912 T16971 T16972 amod adult,mice
R4913 T16972 T16970 dobj mice,housing
R4914 T16973 T16970 prep in,housing
R4915 T16974 T16975 nmod Nalgene,Cages
R4916 T16975 T16973 pobj Cages,in
R4917 T16976 T16975 amod Metabolic,Cages
R4918 T16977 T16978 punct (,Minimitter
R4919 T16978 T16975 parataxis Minimitter,Cages
R492 T2717 T2712 punct ;,translocates
R4920 T16979 T16978 punct ", ",Minimitter
R4921 T16980 T16978 npadvmod Bend,Minimitter
R4922 T16981 T16978 punct ", ",Minimitter
R4923 T16982 T16978 npadvmod Oregon,Minimitter
R4924 T16983 T16978 punct ", ",Minimitter
R4925 T16984 T16985 compound United,States
R4926 T16985 T16978 npadvmod States,Minimitter
R4927 T16986 T16978 punct ),Minimitter
R4928 T16987 T16970 prep for,housing
R4929 T16988 T16989 quantmod 2,3
R493 T2718 T2712 advmod however,translocates
R4930 T16989 T16991 nummod 3,d
R4931 T16990 T16989 punct –,3
R4932 T16991 T16987 pobj d,for
R4933 T16992 T16970 cc and,housing
R4934 T16993 T16970 conj collecting,housing
R4935 T16994 T16993 dobj urine,collecting
R4936 T16995 T16996 det every,period
R4937 T16996 T16993 npadvmod period,collecting
R4938 T16997 T16998 nummod 24,h
R4939 T16998 T16996 compound h,period
R494 T2719 T2712 punct ", ",translocates
R4940 T16999 T16966 punct .,measured
R4941 T17001 T17002 compound Urine,osmolalities
R4942 T17002 T17003 nsubjpass osmolalities,determined
R4943 T17004 T17003 auxpass were,determined
R4944 T17005 T17003 advcl using,determined
R4945 T17006 T17007 det an,Osmometer
R4946 T17007 T17005 dobj Osmometer,using
R4947 T17008 T17009 punct (,Systems
R4948 T17009 T17007 parataxis Systems,Osmometer
R4949 T17010 T17009 dep Osmette,Systems
R495 T2720 T2712 prep under,translocates
R4950 T17011 T17010 nummod 5004,Osmette
R4951 T17012 T17009 punct ;,Systems
R4952 T17013 T17009 compound Precision,Systems
R4953 T17014 T17009 punct ", ",Systems
R4954 T17015 T17009 npadvmod Natick,Systems
R4955 T17016 T17009 punct ", ",Systems
R4956 T17017 T17009 npadvmod Massachusetts,Systems
R4957 T17018 T17009 punct ", ",Systems
R4958 T17019 T17020 compound United,States
R4959 T17020 T17009 npadvmod States,Systems
R496 T2721 T2720 pobj conditions,under
R4960 T17021 T17009 punct ),Systems
R4961 T17022 T17003 punct .,determined
R4962 T17024 T17025 npadvmod Urine,concentrating
R4963 T17025 T17026 amod concentrating,experiments
R4964 T17026 T17027 nsubjpass experiments,carried
R4965 T17028 T17027 auxpass were,carried
R4966 T17029 T17027 prt out,carried
R4967 T17030 T17027 prep by,carried
R4968 T17031 T17032 amod intraperitoneal,injection
R4969 T17032 T17030 pobj injection,by
R497 T2722 T2721 acl requiring,conditions
R4970 T17033 T17032 prep of,injection
R4971 T17034 T17033 pobj dDAVP,of
R4972 T17035 T17036 punct (,μg
R4973 T17036 T17034 parataxis μg,dDAVP
R4974 T17037 T17036 nummod 0.4,μg
R4975 T17038 T17039 punct /,kg
R4976 T17039 T17036 prep kg,μg
R4977 T17040 T17036 punct ),μg
R4978 T17041 T17027 punct .,carried
R4979 T17043 T17044 nsubjpass Mice,injected
R498 T2723 T2724 compound water,retention
R4980 T17045 T17044 auxpass were,injected
R4981 T17046 T17044 advmod twice,injected
R4982 T17047 T17044 prep with,injected
R4983 T17048 T17047 pobj dDAVP,with
R4984 T17049 T17044 punct ", ",injected
R4985 T17050 T17051 advmod once,at
R4986 T17051 T17044 prep at,injected
R4987 T17052 T17051 pobj time,at
R4988 T17053 T17052 nummod 0,time
R4989 T17054 T17051 cc and,at
R499 T2724 T2722 dobj retention,requiring
R4990 T17055 T17056 advmod again,at
R4991 T17056 T17051 conj at,at
R4992 T17057 T17058 nummod 30,min
R4993 T17058 T17056 pobj min,at
R4994 T17059 T17044 punct .,injected
R4995 T17061 T17062 nsubjpass Urine,collected
R4996 T17063 T17062 auxpass was,collected
R4997 T17064 T17062 prep at,collected
R4998 T17065 T17066 det the,start
R4999 T17066 T17064 pobj start,at
R500 T2725 T2712 nsubj AQP2,translocates
R5000 T17067 T17066 prep of,start
R5001 T17068 T17069 det the,experiment
R5002 T17069 T17067 pobj experiment,of
R5003 T17070 T17064 cc and,at
R5004 T17071 T17072 nummod 30,min
R5005 T17072 T17073 npadvmod min,after
R5006 T17073 T17064 conj after,at
R5007 T17074 T17075 det the,injection
R5008 T17075 T17073 pobj injection,after
R5009 T17076 T17075 amod second,injection
R501 T2726 T2712 prep to,translocates
R5010 T17077 T17062 punct .,collected
R5011 T17265 T17266 compound Kidney,membrane
R5012 T17266 T17267 compound membrane,preparation
R5013 T17268 T17267 punct .,preparation
R5014 T17270 T17271 amod Whole,kidneys
R5015 T17271 T17273 nsubjpass kidneys,homogenized
R5016 T17272 T17271 compound mouse,kidneys
R5017 T17274 T17273 auxpass were,homogenized
R5018 T17275 T17273 prep in,homogenized
R5019 T17276 T17277 nummod 10,mM
R502 T2727 T2728 det the,membrane
R5020 T17277 T17278 compound mM,Tris
R5021 T17278 T17275 pobj Tris,in
R5022 T17279 T17278 punct (,Tris
R5023 T17280 T17278 npadvmod pH,Tris
R5024 T17281 T17280 nummod 7.4,pH
R5025 T17282 T17278 punct ),Tris
R5026 T17283 T17278 punct ", ",Tris
R5027 T17284 T17285 nummod 350,mM
R5028 T17285 T17286 compound mM,sucrose
R5029 T17286 T17278 conj sucrose,Tris
R503 T2728 T2726 pobj membrane,to
R5030 T17287 T17286 punct ", ",sucrose
R5031 T17288 T17286 cc and,sucrose
R5032 T17289 T17290 nummod 5,mM
R5033 T17290 T17291 compound mM,EDTA
R5034 T17291 T17286 conj EDTA,sucrose
R5035 T17292 T17291 acl containing,EDTA
R5036 T17293 T17294 compound protease,inhibitors
R5037 T17294 T17292 dobj inhibitors,containing
R5038 T17295 T17296 punct (,P
R5039 T17296 T17294 parataxis P,inhibitors
R504 T2729 T2728 amod apical,membrane
R5040 T17297 T17298 compound Sigma,Aldrich
R5041 T17298 T17296 dep Aldrich,P
R5042 T17299 T17298 punct -,Aldrich
R5043 T17300 T17296 punct ", ",P
R5044 T17301 T17296 punct #,P
R5045 T17302 T17296 punct -,P
R5046 T17303 T17296 nummod 8340,P
R5047 T17304 T17296 punct ),P
R5048 T17305 T17273 prep in,homogenized
R5049 T17306 T17307 det a,homogenizer
R505 T2730 T2712 punct ", ",translocates
R5050 T17307 T17305 pobj homogenizer,in
R5051 T17308 T17309 compound Potter,Elvehjem
R5052 T17309 T17307 compound Elvehjem,homogenizer
R5053 T17310 T17309 punct -,Elvehjem
R5054 T17311 T17273 punct .,homogenized
R5055 T17313 T17314 det The,homogenate
R5056 T17314 T17315 nsubjpass homogenate,centrifuged
R5057 T17316 T17315 auxpass was,centrifuged
R5058 T17317 T17315 prep at,centrifuged
R5059 T17318 T17319 nummod "2,000",g
R506 T2731 T2712 advcl permitting,translocates
R5060 T17319 T17317 pobj g,at
R5061 T17320 T17315 prep for,centrifuged
R5062 T17321 T17322 nummod 10,min
R5063 T17322 T17320 pobj min,for
R5064 T17323 T17315 cc and,centrifuged
R5065 T17324 T17325 det the,supernatant
R5066 T17325 T17326 nsubjpass supernatant,subjected
R5067 T17326 T17315 conj subjected,centrifuged
R5068 T17327 T17326 auxpass was,subjected
R5069 T17328 T17326 prep to,subjected
R507 T2732 T2733 compound water,reabsorption
R5070 T17329 T17328 pobj ultracentrifugation,to
R5071 T17330 T17329 prep at,ultracentrifugation
R5072 T17331 T17332 nummod "100,000",g
R5073 T17332 T17330 pobj g,at
R5074 T17333 T17326 prep for,subjected
R5075 T17334 T17335 nummod 1,h
R5076 T17335 T17333 pobj h,for
R5077 T17336 T17326 prep at,subjected
R5078 T17337 T17338 nummod 4,°C
R5079 T17338 T17336 pobj °C,at
R508 T2733 T2731 dobj reabsorption,permitting
R5080 T17339 T17326 punct .,subjected
R5081 T17341 T17342 amod Pelleted,membranes
R5082 T17342 T17343 nsubjpass membranes,resuspended
R5083 T17344 T17343 auxpass were,resuspended
R5084 T17345 T17343 prep in,resuspended
R5085 T17346 T17347 det the,buffer
R5086 T17347 T17345 pobj buffer,in
R5087 T17348 T17347 amod same,buffer
R5088 T17349 T17343 punct ", ",resuspended
R5089 T17350 T17343 cc and,resuspended
R509 T2734 T2735 punct [,8
R5090 T17351 T17352 compound protein,concentration
R5091 T17352 T17353 nsubjpass concentration,determined
R5092 T17353 T17343 conj determined,resuspended
R5093 T17354 T17353 auxpass was,determined
R5094 T17355 T17353 prep by,determined
R5095 T17356 T17357 compound Bradford,assay
R5096 T17357 T17355 pobj assay,by
R5097 T17358 T17353 punct .,determined
R5098 T17615 T17614 punct .,Immunoblotting
R5099 T17617 T17618 compound Kidney,membrane
R510 T2735 T2712 parataxis 8,translocates
R5100 T17618 T17619 compound membrane,fractions
R5101 T17619 T17620 nsubjpass fractions,resolved
R5102 T17621 T17622 punct (,μg
R5103 T17622 T17619 parataxis μg,fractions
R5104 T17623 T17622 nummod 60,μg
R5105 T17624 T17622 punct ),μg
R5106 T17625 T17620 auxpass were,resolved
R5107 T17626 T17620 prep on,resolved
R5108 T17627 T17628 det a,gel
R5109 T17628 T17626 pobj gel,on
R511 T2736 T2735 nummod 7,8
R5110 T17629 T17630 nummod 12,%
R5111 T17630 T17628 compound %,gel
R5112 T17631 T17632 compound SDS,polyacrylamide
R5113 T17632 T17628 compound polyacrylamide,gel
R5114 T17633 T17632 punct -,polyacrylamide
R5115 T17634 T17620 cc and,resolved
R5116 T17635 T17620 conj transferred,resolved
R5117 T17636 T17635 prep to,transferred
R5118 T17637 T17638 det a,membrane
R5119 T17638 T17636 pobj membrane,to
R512 T2737 T2735 punct ",",8
R5120 T17639 T17638 compound nitrocellulose,membrane
R5121 T17640 T17620 punct .,resolved
R5122 T17642 T17643 nsubjpass Membranes,blocked
R5123 T17644 T17643 auxpass were,blocked
R5124 T17645 T17643 prep in,blocked
R5125 T17646 T17647 nummod 5,%
R5126 T17647 T17648 nmod %,milk
R5127 T17648 T17645 pobj milk,in
R5128 T17649 T17648 amod nonfat,milk
R5129 T17650 T17648 prep in,milk
R513 T2738 T2735 punct ],8
R5130 T17651 T17652 npadvmod Tris,buffered
R5131 T17652 T17654 amod buffered,saline
R5132 T17653 T17652 punct -,buffered
R5133 T17654 T17650 pobj saline,in
R5134 T17655 T17654 prep with,saline
R5135 T17656 T17657 nummod 0.05,%
R5136 T17657 T17658 compound %,Tween
R5137 T17658 T17655 pobj Tween,with
R5138 T17659 T17658 nummod 20,Tween
R5139 T17660 T17661 punct (,TBST
R514 T2739 T2712 punct .,translocates
R5140 T17661 T17658 parataxis TBST,Tween
R5141 T17662 T17661 punct ),TBST
R5142 T17663 T17643 punct ", ",blocked
R5143 T17664 T17643 advcl followed,blocked
R5144 T17665 T17664 agent by,followed
R5145 T17666 T17667 det an,incubation
R5146 T17667 T17665 pobj incubation,by
R5147 T17668 T17667 amod overnight,incubation
R5148 T17669 T17667 punct (,incubation
R5149 T17670 T17667 prep at,incubation
R515 T2741 T2742 mark For,occur
R5150 T17671 T17672 nummod 4,°C
R5151 T17672 T17670 pobj °C,at
R5152 T17673 T17667 punct ),incubation
R5153 T17674 T17667 prep with,incubation
R5154 T17675 T17676 nmod AQP2,antibody
R5155 T17676 T17674 pobj antibody,with
R5156 T17677 T17676 amod polyclonal,antibody
R5157 T17678 T17679 punct (,sc
R5158 T17679 T17676 parataxis sc,antibody
R5159 T17680 T17681 compound Santa,Cruz
R516 T2742 T2746 advcl occur,binds
R5160 T17681 T17682 compound Cruz,Biotechnology
R5161 T17682 T17679 dep Biotechnology,sc
R5162 T17683 T17682 punct ", ",Biotechnology
R5163 T17684 T17685 compound Santa,Cruz
R5164 T17685 T17682 npadvmod Cruz,Biotechnology
R5165 T17686 T17682 punct ", ",Biotechnology
R5166 T17687 T17682 npadvmod California,Biotechnology
R5167 T17688 T17682 punct ", ",Biotechnology
R5168 T17689 T17690 compound United,States
R5169 T17690 T17682 npadvmod States,Biotechnology
R517 T2743 T2744 det this,process
R5170 T17691 T17679 punct ;,sc
R5171 T17692 T17679 punct #,sc
R5172 T17693 T17679 punct -,sc
R5173 T17694 T17679 nummod 9882,sc
R5174 T17695 T17679 punct ),sc
R5175 T17696 T17643 punct .,blocked
R5176 T17698 T17699 nsubjpass Membranes,washed
R5177 T17700 T17699 auxpass were,washed
R5178 T17701 T17699 prep in,washed
R5179 T17702 T17701 pobj TBST,in
R518 T2744 T2742 nsubj process,occur
R5180 T17703 T17704 advmod then,incubated
R5181 T17704 T17699 dep incubated,washed
R5182 T17705 T17704 prep with,incubated
R5183 T17706 T17707 npadvmod HRP,conjugated
R5184 T17707 T17709 amod conjugated,antibody
R5185 T17708 T17707 punct -,conjugated
R5186 T17709 T17705 pobj antibody,with
R5187 T17710 T17709 nmod donkey,antibody
R5188 T17711 T17709 amod anti-goat,antibody
R5189 T17712 T17699 punct .,washed
R519 T2745 T2742 aux to,occur
R5190 T17714 T17715 nsubjpass Membranes,washed
R5191 T17716 T17715 auxpass were,washed
R5192 T17717 T17715 advmod further,washed
R5193 T17718 T17715 prep in,washed
R5194 T17719 T17718 pobj TBST,in
R5195 T17720 T17715 cc and,washed
R5196 T17721 T17722 nsubjpass bands,visualized
R5197 T17722 T17715 conj visualized,washed
R5198 T17723 T17722 auxpass were,visualized
R5199 T17724 T17722 advcl using,visualized
R520 T2747 T2746 punct ", ",binds
R5200 T17725 T17726 compound ECL,reagent
R5201 T17726 T17724 dobj reagent,using
R5202 T17727 T17728 punct (,Biosciences
R5203 T17728 T17726 parataxis Biosciences,reagent
R5204 T17729 T17728 compound Amersham,Biosciences
R5205 T17730 T17728 punct ", ",Biosciences
R5206 T17731 T17732 compound Little,Chalfont
R5207 T17732 T17728 npadvmod Chalfont,Biosciences
R5208 T17733 T17728 punct ", ",Biosciences
R5209 T17734 T17735 compound United,Kingdom
R521 T2748 T2746 nsubj AVP,binds
R5210 T17735 T17728 npadvmod Kingdom,Biosciences
R5211 T17736 T17728 punct ),Biosciences
R5212 T17737 T17722 punct .,visualized
R5213 T17905 T17906 compound Endoglycosidase,digestion
R5214 T17907 T17906 punct .,digestion
R5215 T17909 T17910 compound Kidney,membranes
R5216 T17910 T17911 nsubjpass membranes,incubated
R5217 T17912 T17913 punct (,μg
R5218 T17913 T17910 parataxis μg,membranes
R5219 T17914 T17913 nummod 60,μg
R522 T2749 T2750 poss its,receptor
R5220 T17915 T17913 punct ),μg
R5221 T17916 T17911 auxpass were,incubated
R5222 T17917 T17911 prep in,incubated
R5223 T17918 T17919 nummod 50,mM
R5224 T17919 T17920 compound mM,phosphate
R5225 T17920 T17917 pobj phosphate,in
R5226 T17921 T17920 compound sodium,phosphate
R5227 T17922 T17920 punct (,phosphate
R5228 T17923 T17920 npadvmod pH,phosphate
R5229 T17924 T17923 nummod 5.5,pH
R523 T2750 T2746 dobj receptor,binds
R5230 T17925 T17920 punct ),phosphate
R5231 T17926 T17920 punct ", ",phosphate
R5232 T17927 T17928 nummod 0.1,%
R5233 T17928 T17929 compound %,SDS
R5234 T17929 T17920 appos SDS,phosphate
R5235 T17930 T17929 punct ", ",SDS
R5236 T17931 T17929 cc and,SDS
R5237 T17932 T17933 nummod 50,mM
R5238 T17933 T17934 compound mM,mercaptoethanol
R5239 T17934 T17929 conj mercaptoethanol,SDS
R524 T2751 T2750 punct ", ",receptor
R5240 T17935 T17934 compound β,mercaptoethanol
R5241 T17936 T17934 punct -,mercaptoethanol
R5242 T17937 T17911 punct ", ",incubated
R5243 T17938 T17911 dep heated,incubated
R5244 T17939 T17938 prep to,heated
R5245 T17940 T17941 nummod 100,°C
R5246 T17941 T17939 pobj °C,to
R5247 T17942 T17938 prep for,heated
R5248 T17943 T17944 nummod 5,min
R5249 T17944 T17942 pobj min,for
R525 T2752 T2750 appos AVPR2,receptor
R5250 T17945 T17911 punct ", ",incubated
R5251 T17946 T17947 advmod then,cooled
R5252 T17947 T17911 dep cooled,incubated
R5253 T17948 T17911 punct .,incubated
R5254 T17950 T17951 compound Endoglycosidase,H
R5255 T17951 T17952 nsubjpass H,added
R5256 T17953 T17954 punct (,Aldrich
R5257 T17954 T17951 parataxis Aldrich,H
R5258 T17955 T17956 nummod 0.01,units
R5259 T17956 T17954 dep units,Aldrich
R526 T2753 T2746 punct ", ",binds
R5260 T17957 T17954 punct ;,Aldrich
R5261 T17958 T17954 compound Sigma,Aldrich
R5262 T17959 T17954 punct -,Aldrich
R5263 T17960 T17954 punct ),Aldrich
R5264 T17961 T17952 auxpass was,added
R5265 T17962 T17952 cc and,added
R5266 T17963 T17952 conj incubated,added
R5267 T17964 T17963 prep at,incubated
R5268 T17965 T17966 nummod 37,°C
R5269 T17966 T17964 pobj °C,at
R527 T2754 T2746 prep on,binds
R5270 T17967 T17963 prep for,incubated
R5271 T17968 T17969 nummod 2,h
R5272 T17969 T17967 pobj h,for
R5273 T17970 T17952 punct .,added
R5274 T17972 T17973 det The,reaction
R5275 T17973 T17974 nsubjpass reaction,stopped
R5276 T17975 T17974 auxpass was,stopped
R5277 T17976 T17974 prep by,stopped
R5278 T17977 T17976 pcomp boiling,by
R5279 T17978 T17979 det the,samples
R528 T2755 T2756 det the,face
R5280 T17979 T17977 dobj samples,boiling
R5281 T17980 T17977 prep in,boiling
R5282 T17981 T17982 compound Laemmli,buffer
R5283 T17982 T17980 pobj buffer,in
R5284 T17983 T17974 punct .,stopped
R5285 T17985 T17986 amod Total,reactants
R5286 T17986 T17987 nsubjpass reactants,immunoblotted
R5287 T17988 T17987 auxpass were,immunoblotted
R5288 T17989 T17990 mark as,described
R5289 T17990 T17987 advcl described,immunoblotted
R529 T2756 T2754 pobj face,on
R5290 T17991 T17990 advmod above,described
R5291 T17992 T17987 punct .,immunoblotted
R5292 T18784 T18783 cc and,Coimmunoprecipitation
R5293 T18785 T18783 conj biotinylation,Coimmunoprecipitation
R5294 T18786 T18783 prep in,Coimmunoprecipitation
R5295 T18787 T18788 compound MDCK,cells
R5296 T18788 T18786 pobj cells,in
R5297 T18789 T18783 punct .,Coimmunoprecipitation
R5298 T18791 T18792 compound MDCK,cells
R5299 T18792 T18793 nsubjpass cells,transfected
R530 T2757 T2756 amod basolateral,face
R5300 T18794 T18795 advmod stably,expressing
R5301 T18795 T18792 acl expressing,cells
R5302 T18796 T18797 amod wild,type
R5303 T18797 T18799 compound type,AQP2
R5304 T18798 T18797 punct -,type
R5305 T18799 T18795 dobj AQP2,expressing
R5306 T18800 T18799 punct (,AQP2
R5307 T18801 T18799 acl grown,AQP2
R5308 T18802 T18801 prep on,grown
R5309 T18803 T18804 nummod 10,cm
R531 T2758 T2756 prep of,face
R5310 T18804 T18806 compound cm,plates
R5311 T18805 T18804 punct -,cm
R5312 T18806 T18802 pobj plates,on
R5313 T18807 T18795 punct ),expressing
R5314 T18808 T18793 auxpass were,transfected
R5315 T18809 T18793 prep with,transfected
R5316 T18810 T18811 nmod pEGFP,type
R5317 T18811 T18815 compound type,AQP2
R5318 T18812 T18811 punct -,type
R5319 T18813 T18811 amod wild,type
R532 T2759 T2760 det the,cells
R5320 T18814 T18811 punct -,type
R5321 T18815 T18809 pobj AQP2,with
R5322 T18816 T18815 punct ", ",AQP2
R5323 T18817 T18818 compound pEGFP,F204V
R5324 T18818 T18815 conj F204V,AQP2
R5325 T18819 T18818 punct -,F204V
R5326 T18820 T18818 compound AQP2,F204V
R5327 T18821 T18818 punct -,F204V
R5328 T18822 T18818 punct ", ",F204V
R5329 T18823 T18818 cc or,F204V
R533 T2760 T2758 pobj cells,of
R5330 T18824 T18818 conj vector,F204V
R5331 T18825 T18824 advmod alone,vector
R5332 T18826 T18793 punct .,transfected
R5333 T18828 T18829 det The,cells
R5334 T18829 T18830 nsubjpass cells,homogenized
R5335 T18831 T18830 auxpass were,homogenized
R5336 T18832 T18830 prep in,homogenized
R5337 T18833 T18834 nummod 10,mM
R5338 T18834 T18835 compound mM,Tris
R5339 T18835 T18832 pobj Tris,in
R534 T2761 T2762 amod collecting,duct
R5340 T18836 T18835 punct (,Tris
R5341 T18837 T18835 npadvmod pH,Tris
R5342 T18838 T18837 nummod 7.4,pH
R5343 T18839 T18835 punct ),Tris
R5344 T18840 T18835 punct ", ",Tris
R5345 T18841 T18842 nummod 1,mM
R5346 T18842 T18843 compound mM,EDTA
R5347 T18843 T18835 conj EDTA,Tris
R5348 T18844 T18843 punct ", ",EDTA
R5349 T18845 T18843 cc and,EDTA
R535 T2762 T2760 compound duct,cells
R5350 T18846 T18847 nummod 250,mM
R5351 T18847 T18848 compound mM,sucrose
R5352 T18848 T18843 conj sucrose,EDTA
R5353 T18849 T18850 nummod 40,h
R5354 T18850 T18851 npadvmod h,later
R5355 T18851 T18830 advmod later,homogenized
R5356 T18852 T18830 punct .,homogenized
R5357 T18854 T18855 det The,supernatant
R5358 T18855 T18857 nsubjpass supernatant,centrifuged
R5359 T18856 T18855 amod clarified,supernatant
R536 T2763 T2746 punct ", ",binds
R5360 T18858 T18857 auxpass was,centrifuged
R5361 T18859 T18857 prep at,centrifuged
R5362 T18860 T18861 nummod "200,000",g
R5363 T18861 T18859 pobj g,at
R5364 T18862 T18857 prep for,centrifuged
R5365 T18863 T18864 nummod 30,min
R5366 T18864 T18862 pobj min,for
R5367 T18865 T18857 punct .,centrifuged
R5368 T18867 T18868 amod Pelleted,membranes
R5369 T18868 T18869 nsubjpass membranes,incubated
R537 T2764 T2746 advcl leading,binds
R5370 T18870 T18869 auxpass were,incubated
R5371 T18871 T18869 aux resuspended,incubated
R5372 T18872 T18869 prep in,incubated
R5373 T18873 T18874 det the,buffer
R5374 T18874 T18872 pobj buffer,in
R5375 T18875 T18874 amod same,buffer
R5376 T18876 T18874 prep but,buffer
R5377 T18877 T18876 pcomp containing,but
R5378 T18878 T18879 nummod 4,%
R5379 T18879 T18880 compound %,deoxycholate
R538 T2765 T2764 prep to,leading
R5380 T18880 T18877 dobj deoxycholate,containing
R5381 T18881 T18880 compound sodium,deoxycholate
R5382 T18882 T18869 cc and,incubated
R5383 T18883 T18869 prep at,incubated
R5384 T18884 T18885 nummod 37,°C
R5385 T18885 T18883 pobj °C,at
R5386 T18886 T18869 prep for,incubated
R5387 T18887 T18888 nummod 1,h
R5388 T18888 T18886 pobj h,for
R5389 T18889 T18869 punct .,incubated
R539 T2766 T2767 det a,rise
R5390 T18891 T18892 prep From,removed
R5391 T18893 T18894 det the,membranes
R5392 T18894 T18891 pobj membranes,From
R5393 T18895 T18894 amod dissolved,membranes
R5394 T18896 T18892 punct ", ",removed
R5395 T18897 T18898 det a,sample
R5396 T18898 T18892 nsubjpass sample,removed
R5397 T18899 T18900 nummod 30,μl
R5398 T18900 T18898 compound μl,sample
R5399 T18901 T18892 auxpass was,removed
R540 T2767 T2765 pobj rise,to
R5400 T18902 T18892 cc and,removed
R5401 T18903 T18892 conj used,removed
R5402 T18904 T18903 prep as,used
R5403 T18905 T18906 det the,fraction
R5404 T18906 T18904 pobj fraction,as
R5405 T18907 T18906 amod total,fraction
R5406 T18908 T18906 compound membrane,fraction
R5407 T18909 T18892 punct .,removed
R5408 T18911 T18912 det The,membranes
R5409 T18912 T18914 nsubjpass membranes,diluted
R541 T2768 T2767 prep in,rise
R5410 T18913 T18912 amod remaining,membranes
R5411 T18915 T18914 auxpass were,diluted
R5412 T18916 T18914 prep with,diluted
R5413 T18917 T18918 nummod 600,μl
R5414 T18918 T18916 pobj μl,with
R5415 T18919 T18918 prep of,μl
R5416 T18920 T18921 det the,buffer
R5417 T18921 T18919 pobj buffer,of
R5418 T18922 T18921 compound homogenization,buffer
R5419 T18923 T18914 punct ", ",diluted
R542 T2769 T2770 amod intracellular,cAMP
R5420 T18924 T18914 cc and,diluted
R5421 T18925 T18914 conj incubated,diluted
R5422 T18926 T18925 prep with,incubated
R5423 T18927 T18928 nummod 1,μl
R5424 T18928 T18926 pobj μl,with
R5425 T18929 T18928 prep of,μl
R5426 T18930 T18931 compound GFP,antisera
R5427 T18931 T18929 pobj antisera,of
R5428 T18932 T18933 punct (,0092
R5429 T18933 T18931 parataxis 0092,antisera
R543 T2770 T2768 pobj cAMP,in
R5430 T18934 T18933 dep Invitrogen,0092
R5431 T18935 T18933 punct ", ",0092
R5432 T18936 T18933 punct #,0092
R5433 T18937 T18933 nummod 46,0092
R5434 T18938 T18933 punct –,0092
R5435 T18939 T18933 punct ),0092
R5436 T18940 T18931 cc and,antisera
R5437 T18941 T18942 compound protein,sepharose
R5438 T18942 T18931 conj sepharose,antisera
R5439 T18943 T18944 compound A,G
R544 T2771 T2764 punct ", ",leading
R5440 T18944 T18942 compound G,sepharose
R5441 T18945 T18944 punct /,G
R5442 T18946 T18914 punct .,diluted
R5443 T18948 T18949 prep Following,washed
R5444 T18950 T18951 amod overnight,incubation
R5445 T18951 T18948 pobj incubation,Following
R5446 T18952 T18949 punct ", ",washed
R5447 T18953 T18954 det the,proteins
R5448 T18954 T18949 nsubjpass proteins,washed
R5449 T18955 T18954 amod precipitated,proteins
R545 T2772 T2773 advmod ultimately,resulting
R5450 T18956 T18949 auxpass were,washed
R5451 T18957 T18949 prep in,washed
R5452 T18958 T18959 compound RIPA,buffer
R5453 T18959 T18957 pobj buffer,in
R5454 T18960 T18949 cc and,washed
R5455 T18961 T18962 advmod finally,boiled
R5456 T18962 T18949 conj boiled,washed
R5457 T18963 T18962 prep in,boiled
R5458 T18964 T18965 nummod 50,μl
R5459 T18965 T18963 pobj μl,in
R546 T2773 T2764 advcl resulting,leading
R5460 T18966 T18965 prep of,μl
R5461 T18967 T18968 compound Laemmli,buffer
R5462 T18968 T18966 pobj buffer,of
R5463 T18969 T18949 punct .,washed
R5464 T18971 T18972 nsubjpass Half,processed
R5465 T18973 T18971 prep of,Half
R5466 T18974 T18975 det the,membrane
R5467 T18975 T18973 pobj membrane,of
R5468 T18976 T18975 amod total,membrane
R5469 T18977 T18975 cc and,membrane
R547 T2774 T2773 prep in,resulting
R5470 T18978 T18979 det the,fractions
R5471 T18979 T18975 conj fractions,membrane
R5472 T18980 T18979 compound IP,fractions
R5473 T18981 T18972 auxpass were,processed
R5474 T18982 T18972 prep for,processed
R5475 T18983 T18982 pobj immunoblotting,for
R5476 T18984 T18972 punct .,processed
R5477 T18986 T18987 compound Cell,biotinylation
R5478 T18987 T18989 nsubjpass biotinylation,performed
R5479 T18988 T18987 compound surface,biotinylation
R548 T2775 T2774 pobj phosphorylation,in
R5480 T18990 T18989 auxpass was,performed
R5481 T18991 T18989 prep in,performed
R5482 T18992 T18993 det a,manner
R5483 T18993 T18991 pobj manner,in
R5484 T18994 T18993 amod similar,manner
R5485 T18995 T18989 punct .,performed
R5486 T18997 T18998 advmod However,transfected
R5487 T18999 T18998 punct ", ",transfected
R5488 T19000 T19001 compound pEGFP,F204V
R5489 T19001 T18998 nsubjpass F204V,transfected
R549 T2776 T2775 prep of,phosphorylation
R5490 T19002 T19001 punct -,F204V
R5491 T19003 T19001 compound AQP2,F204V
R5492 T19004 T19001 punct -,F204V
R5493 T19005 T18998 punct ", ",transfected
R5494 T19006 T18998 auxpass was,transfected
R5495 T19007 T18998 prep into,transfected
R5496 T19008 T19009 compound MDCK,cells
R5497 T19009 T19007 pobj cells,into
R5498 T19010 T19011 advmod stably,expressing
R5499 T19011 T19009 acl expressing,cells
R550 T2777 T2776 pobj AQP2,of
R5500 T19012 T19013 amod wild,type
R5501 T19013 T19015 compound type,AQP2
R5502 T19014 T19013 punct -,type
R5503 T19015 T19011 dobj AQP2,expressing
R5504 T19016 T19009 cc and,cells
R5505 T19017 T19009 conj cells,cells
R5506 T19018 T19017 acl made,cells
R5507 T19019 T19018 oprd stable,made
R5508 T19020 T19018 prep with,made
R5509 T19021 T19020 pobj vector,with
R551 T2778 T2775 prep at,phosphorylation
R5510 T19022 T19021 advmod alone,vector
R5511 T19023 T18998 punct .,transfected
R5512 T19025 T19026 compound Twenty,four
R5513 T19026 T19028 nummod four,hours
R5514 T19027 T19026 punct -,four
R5515 T19028 T19029 npadvmod hours,post-transfection
R5516 T19029 T19030 advmod post-transfection,stimulated
R5517 T19031 T19030 punct ", ",stimulated
R5518 T19032 T19030 nsubjpass cells,stimulated
R5519 T19033 T19030 auxpass were,stimulated
R552 T2779 T2778 pobj serine,at
R5520 T19034 T19030 prep with,stimulated
R5521 T19035 T19034 pobj forskolin,with
R5522 T19036 T19030 punct ", ",stimulated
R5523 T19037 T19030 conj trypsinized,stimulated
R5524 T19038 T19037 punct ", ",trypsinized
R5525 T19039 T19037 conj resuspended,trypsinized
R5526 T19040 T19039 prep in,resuspended
R5527 T19041 T19042 nummod 1,ml
R5528 T19042 T19040 pobj ml,in
R5529 T19043 T19042 prep of,ml
R553 T2780 T2779 nummod 256,serine
R5530 T19044 T19043 pobj PBS,of
R5531 T19045 T19046 punct (,cells
R5532 T19046 T19042 parataxis cells,ml
R5533 T19047 T19048 quantmod 2.5,106
R5534 T19048 T19046 nummod 106,cells
R5535 T19049 T19048 punct ×,106
R5536 T19050 T19051 punct /,ml
R5537 T19051 T19046 prep ml,cells
R5538 T19052 T19046 punct ),cells
R5539 T19053 T19039 punct ", ",resuspended
R554 T2781 T2775 prep by,phosphorylation
R5540 T19054 T19039 cc and,resuspended
R5541 T19055 T19039 conj incubated,resuspended
R5542 T19056 T19055 prep with,incubated
R5543 T19057 T19058 nummod 0.5,mg
R5544 T19058 T19056 pobj mg,with
R5545 T19059 T19058 prep of,mg
R5546 T19060 T19061 compound NHS,biotin
R5547 T19061 T19059 pobj biotin,of
R5548 T19062 T19061 punct -,biotin
R5549 T19063 T19061 compound PEO4,biotin
R555 T2782 T2783 npadvmod cAMP,dependent
R5550 T19064 T19061 punct -,biotin
R5551 T19065 T19066 punct (,Biotechnology
R5552 T19066 T19061 parataxis Biotechnology,biotin
R5553 T19067 T19066 compound Pierce,Biotechnology
R5554 T19068 T19066 punct ),Biotechnology
R5555 T19069 T19055 prep for,incubated
R5556 T19070 T19071 nummod 30,min
R5557 T19071 T19069 pobj min,for
R5558 T19072 T19055 prep at,incubated
R5559 T19073 T19074 compound room,temperature
R556 T2783 T2785 amod dependent,kinase
R5560 T19074 T19072 pobj temperature,at
R5561 T19075 T19030 punct .,stimulated
R5562 T19077 T19078 nsubj Cells,washed
R5563 T19079 T19078 aux were,washed
R5564 T19080 T19078 advmod once,washed
R5565 T19081 T19078 prep in,washed
R5566 T19082 T19083 nummod 10,mM
R5567 T19083 T19084 compound mM,Tris
R5568 T19084 T19081 pobj Tris,in
R5569 T19085 T19086 punct (,pH
R557 T2784 T2783 punct -,dependent
R5570 T19086 T19084 parataxis pH,Tris
R5571 T19087 T19086 nummod 8,pH
R5572 T19088 T19086 punct ),pH
R5573 T19089 T19078 cc and,washed
R5574 T19090 T19091 nummod three,times
R5575 T19091 T19092 npadvmod times,in
R5576 T19092 T19078 conj in,washed
R5577 T19093 T19092 pobj PBS,in
R5578 T19094 T19078 punct ", ",washed
R5579 T19095 T19096 prep after,purified
R558 T2785 T2781 pobj kinase,by
R5580 T19096 T19078 advcl purified,washed
R5581 T19097 T19095 pobj which,after
R5582 T19098 T19096 nsubjpass membranes,purified
R5583 T19099 T19096 auxpass were,purified
R5584 T19100 T19096 cc and,purified
R5585 T19101 T19096 conj solubilized,purified
R5586 T19102 T19103 mark as,described
R5587 T19103 T19101 advcl described,solubilized
R5588 T19104 T19103 advmod above,described
R5589 T19105 T19078 punct .,washed
R559 T2786 T2785 compound protein,kinase
R5590 T19107 T19108 amod Solubilized,membranes
R5591 T19108 T19109 nsubjpass membranes,incubated
R5592 T19110 T19109 auxpass were,incubated
R5593 T19111 T19109 prep with,incubated
R5594 T19112 T19113 nummod 20,μl
R5595 T19113 T19111 pobj μl,with
R5596 T19114 T19113 prep of,μl
R5597 T19115 T19116 amod immobilized,streptavidin
R5598 T19116 T19114 pobj streptavidin,of
R5599 T19117 T19118 punct (,Biotechnology
R560 T2787 T2788 punct [,9
R5600 T19118 T19116 parataxis Biotechnology,streptavidin
R5601 T19119 T19118 compound Pierce,Biotechnology
R5602 T19120 T19118 punct ),Biotechnology
R5603 T19121 T19109 prep for,incubated
R5604 T19122 T19123 nummod 2,h
R5605 T19123 T19121 pobj h,for
R5606 T19124 T19109 prep at,incubated
R5607 T19125 T19126 nummod 4,°C
R5608 T19126 T19124 pobj °C,at
R5609 T19127 T19109 punct .,incubated
R561 T2788 T2775 parataxis 9,phosphorylation
R5610 T19129 T19130 advmod Finally,washed
R5611 T19131 T19132 det the,proteins
R5612 T19132 T19130 nsubjpass proteins,washed
R5613 T19133 T19132 amod precipitated,proteins
R5614 T19134 T19130 auxpass were,washed
R5615 T19135 T19130 prep in,washed
R5616 T19136 T19137 compound RIPA,buffer
R5617 T19137 T19135 pobj buffer,in
R5618 T19138 T19130 cc and,washed
R5619 T19139 T19130 conj boiled,washed
R562 T2789 T2788 punct ],9
R5620 T19140 T19139 prep in,boiled
R5621 T19141 T19142 nummod 50,μl
R5622 T19142 T19140 pobj μl,in
R5623 T19143 T19142 prep of,μl
R5624 T19144 T19145 compound Laemmli,buffer
R5625 T19145 T19143 pobj buffer,of
R5626 T19146 T19130 punct .,washed
R5627 T19148 T19149 amod Total,cells
R5628 T19149 T19150 nsubj cells,immunoblotting
R5629 T19151 T19149 cc and,cells
R563 T2790 T2775 cc and,phosphorylation
R5630 T19152 T19153 det the,precipitates
R5631 T19153 T19149 conj precipitates,cells
R5632 T19154 T19153 amod biotinylated,precipitates
R5633 T19155 T19150 aux were,immunoblotting
R5634 T19156 T19150 advcl using,immunoblotting
R5635 T19157 T19158 det an,antibody
R5636 T19158 T19156 dobj antibody,using
R5637 T19159 T19158 prep to,antibody
R5638 T19160 T19159 pobj AQP2,to
R5639 T19161 T19150 punct .,immunoblotting
R564 T2791 T2792 poss its,redistribution
R5640 T19603 T19604 compound Kidney,immunohistochemistry
R5641 T19605 T19604 punct .,immunohistochemistry
R5642 T19607 T19608 amod Whole,kidneys
R5643 T19608 T19610 nsubjpass kidneys,fixed
R5644 T19609 T19608 compound mouse,kidneys
R5645 T19611 T19610 auxpass were,fixed
R5646 T19612 T19610 prep in,fixed
R5647 T19613 T19614 nummod 10,%
R5648 T19614 T19615 nmod %,formalin
R5649 T19615 T19612 pobj formalin,in
R565 T2792 T2775 conj redistribution,phosphorylation
R5650 T19616 T19617 npadvmod phosphate,buffered
R5651 T19617 T19615 amod buffered,formalin
R5652 T19618 T19617 punct -,buffered
R5653 T19619 T19610 prep for,fixed
R5654 T19620 T19621 nummod 24,h
R5655 T19621 T19619 pobj h,for
R5656 T19622 T19610 punct .,fixed
R5657 T19624 T19625 nsubjpass Kidneys,embedded
R5658 T19626 T19625 auxpass were,embedded
R5659 T19627 T19625 prep in,embedded
R566 T2793 T2792 prep to,redistribution
R5660 T19628 T19627 pobj paraffin,in
R5661 T19629 T19625 punct ", ",embedded
R5662 T19630 T19625 cc and,embedded
R5663 T19631 T19632 nummod 5,μm
R5664 T19632 T19634 compound μm,sections
R5665 T19633 T19632 punct -,μm
R5666 T19634 T19635 nsubjpass sections,prepared
R5667 T19635 T19625 conj prepared,embedded
R5668 T19636 T19635 auxpass were,prepared
R5669 T19637 T19635 punct .,prepared
R567 T2794 T2795 det the,membrane
R5670 T19639 T19640 prep Following,probed
R5671 T19641 T19642 compound antigen,retrieval
R5672 T19642 T19639 pobj retrieval,Following
R5673 T19643 T19642 acl using,retrieval
R5674 T19644 T19645 nummod 10,mM
R5675 T19645 T19646 compound mM,citrate
R5676 T19646 T19643 dobj citrate,using
R5677 T19647 T19646 compound sodium,citrate
R5678 T19648 T19646 punct (,citrate
R5679 T19649 T19646 npadvmod pH,citrate
R568 T2795 T2793 pobj membrane,to
R5680 T19650 T19649 nummod 8,pH
R5681 T19651 T19646 punct ),citrate
R5682 T19652 T19643 prep for,using
R5683 T19653 T19654 nummod 10,min
R5684 T19654 T19652 pobj min,for
R5685 T19655 T19643 prep at,using
R5686 T19656 T19657 nummod 98,°C
R5687 T19657 T19655 pobj °C,at
R5688 T19658 T19640 punct ", ",probed
R5689 T19659 T19640 nsubjpass sections,probed
R569 T2796 T2795 compound plasma,membrane
R5690 T19660 T19640 auxpass were,probed
R5691 T19661 T19640 advmod sequentially,probed
R5692 T19662 T19640 punct ", ",probed
R5693 T19663 T19664 advmod first,for
R5694 T19664 T19640 prep for,probed
R5695 T19665 T19664 pobj AQP3,for
R5696 T19666 T19664 cc and,for
R5697 T19667 T19668 advmod then,for
R5698 T19668 T19664 conj for,for
R5699 T19669 T19668 pobj AQP2,for
R570 T2797 T2746 punct .,binds
R5700 T19670 T19640 punct .,probed
R5701 T19672 T19673 nsubjpass Sections,incubated
R5702 T19674 T19673 auxpass were,incubated
R5703 T19675 T19673 prep in,incubated
R5704 T19676 T19677 nummod 5,%
R5705 T19677 T19678 compound %,serum
R5706 T19678 T19675 pobj serum,in
R5707 T19679 T19678 compound donkey,serum
R5708 T19680 T19673 cc and,incubated
R5709 T19681 T19682 advmod then,in
R571 T2799 T2800 det The,importance
R5710 T19682 T19673 conj in,incubated
R5711 T19683 T19684 nmod goat,antibody
R5712 T19684 T19682 pobj antibody,in
R5713 T19685 T19684 amod anti-AQP3,antibody
R5714 T19686 T19687 punct (,sc
R5715 T19687 T19684 parataxis sc,antibody
R5716 T19688 T19687 dep 1,sc
R5717 T19689 T19690 punct :,100
R5718 T19690 T19688 prep 100,1
R5719 T19691 T19687 punct ;,sc
R572 T2800 T2801 nsubjpass importance,highlighted
R5720 T19692 T19693 compound Santa,Cruz
R5721 T19693 T19694 compound Cruz,Biotechnology
R5722 T19694 T19687 dep Biotechnology,sc
R5723 T19695 T19687 punct ;,sc
R5724 T19696 T19687 punct #,sc
R5725 T19697 T19687 punct -,sc
R5726 T19698 T19687 nummod 9885,sc
R5727 T19699 T19687 punct ),sc
R5728 T19700 T19673 punct .,incubated
R5729 T19702 T19703 nsubjpass Slides,washed
R573 T2802 T2800 prep of,importance
R5730 T19704 T19703 auxpass were,washed
R5731 T19705 T19703 prep with,washed
R5732 T19706 T19705 pobj PBS,with
R5733 T19707 T19703 cc and,washed
R5734 T19708 T19703 conj incubated,washed
R5735 T19709 T19708 prep with,incubated
R5736 T19710 T19711 npadvmod AlexaFluor,conjugated
R5737 T19711 T19714 amod conjugated,antibody
R5738 T19712 T19710 nummod 488,AlexaFluor
R5739 T19713 T19711 punct -,conjugated
R574 T2803 T2804 compound AQP2,redistribution
R5740 T19714 T19709 pobj antibody,with
R5741 T19715 T19714 nmod donkey,antibody
R5742 T19716 T19714 amod anti-goat,antibody
R5743 T19717 T19718 punct (,Probes
R5744 T19718 T19714 parataxis Probes,antibody
R5745 T19719 T19718 compound Molecular,Probes
R5746 T19720 T19718 punct ", ",Probes
R5747 T19721 T19718 npadvmod Eugene,Probes
R5748 T19722 T19718 punct ", ",Probes
R5749 T19723 T19718 npadvmod Oregon,Probes
R575 T2804 T2802 pobj redistribution,of
R5750 T19724 T19718 punct ", ",Probes
R5751 T19725 T19726 compound United,States
R5752 T19726 T19718 npadvmod States,Probes
R5753 T19727 T19718 punct ),Probes
R5754 T19728 T19703 punct .,washed
R5755 T19730 T19731 det The,slides
R5756 T19731 T19732 nsubjpass slides,incubated
R5757 T19733 T19732 auxpass were,incubated
R5758 T19734 T19732 advmod subsequently,incubated
R5759 T19735 T19732 aux blocked,incubated
R576 T2805 T2801 aux has,highlighted
R5760 T19736 T19732 prep in,incubated
R5761 T19737 T19738 nummod 5,%
R5762 T19738 T19739 compound %,serum
R5763 T19739 T19736 pobj serum,in
R5764 T19740 T19739 compound chicken,serum
R5765 T19741 T19732 punct ", ",incubated
R5766 T19742 T19732 prep with,incubated
R5767 T19743 T19744 det a,antibody
R5768 T19744 T19742 pobj antibody,with
R5769 T19745 T19744 nmod rabbit,antibody
R577 T2806 T2801 auxpass been,highlighted
R5770 T19746 T19744 amod anti-AQP2,antibody
R5771 T19747 T19748 punct (,A3000
R5772 T19748 T19744 parataxis A3000,antibody
R5773 T19749 T19748 dep 1,A3000
R5774 T19750 T19751 punct :,250
R5775 T19751 T19749 prep 250,1
R5776 T19752 T19748 punct ;,A3000
R5777 T19753 T19748 dep USB,A3000
R5778 T19754 T19753 punct ", ",USB
R5779 T19755 T19753 npadvmod Cleveland,USB
R578 T2807 T2801 agent by,highlighted
R5780 T19756 T19753 punct ", ",USB
R5781 T19757 T19753 npadvmod Ohio,USB
R5782 T19758 T19753 punct ", ",USB
R5783 T19759 T19760 compound United,States
R5784 T19760 T19753 npadvmod States,USB
R5785 T19761 T19748 punct ;,A3000
R5786 T19762 T19748 punct #,A3000
R5787 T19763 T19748 punct –,A3000
R5788 T19764 T19748 nummod 06,A3000
R5789 T19765 T19748 punct ),A3000
R579 T2808 T2809 amod functional,characterization
R5790 T19766 T19744 punct ", ",antibody
R5791 T19767 T19768 dep which,detected
R5792 T19768 T19744 relcl detected,antibody
R5793 T19769 T19768 auxpass was,detected
R5794 T19770 T19768 prep with,detected
R5795 T19771 T19772 det a,antibody
R5796 T19772 T19770 pobj antibody,with
R5797 T19773 T19774 npadvmod AlexaFluor,conjugated
R5798 T19774 T19772 amod conjugated,antibody
R5799 T19775 T19773 nummod 594,AlexaFluor
R580 T2809 T2807 pobj characterization,by
R5800 T19776 T19774 punct -,conjugated
R5801 T19777 T19772 nmod chicken,antibody
R5802 T19778 T19772 amod anti-rabbit,antibody
R5803 T19779 T19780 punct (,Probes
R5804 T19780 T19772 parataxis Probes,antibody
R5805 T19781 T19780 dep 1,Probes
R5806 T19782 T19783 punct :,500
R5807 T19783 T19781 prep 500,1
R5808 T19784 T19780 punct ;,Probes
R5809 T19785 T19780 compound Molecular,Probes
R581 T2810 T2809 prep of,characterization
R5810 T19786 T19780 punct ),Probes
R5811 T19787 T19732 punct .,incubated
R5812 T19789 T19790 det The,sections
R5813 T19790 T19791 nsubjpass sections,stained
R5814 T19792 T19791 auxpass were,stained
R5815 T19793 T19791 prep with,stained
R5816 T19794 T19793 pobj DAPI,with
R5817 T19795 T19791 cc and,stained
R5818 T19796 T19791 conj mounted,stained
R5819 T19797 T19796 prep in,mounted
R582 T2811 T2812 compound Aqp2,mutations
R5820 T19798 T19797 pobj Vectashield,in
R5821 T19799 T19800 punct (,Labs
R5822 T19800 T19796 parataxis Labs,mounted
R5823 T19801 T19800 compound Vector,Labs
R5824 T19802 T19800 punct ", ",Labs
R5825 T19803 T19800 npadvmod Burlingame,Labs
R5826 T19804 T19800 punct ", ",Labs
R5827 T19805 T19800 npadvmod California,Labs
R5828 T19806 T19800 punct ", ",Labs
R5829 T19807 T19808 compound United,States
R583 T2812 T2810 pobj mutations,of
R5830 T19808 T19800 npadvmod States,Labs
R5831 T19809 T19800 punct ),Labs
R5832 T19810 T19791 punct .,stained
R5833 T20425 T20424 prep on,Immunocytochemistry
R5834 T20426 T20427 compound MDCK,cells
R5835 T20427 T20425 pobj cells,on
R5836 T20428 T20424 punct .,Immunocytochemistry
R5837 T20430 T20431 nmod MDCK,lines
R5838 T20431 T20434 nsubjpass lines,grown
R5839 T20432 T20431 amod stable,lines
R584 T2813 T2801 advcl resulting,highlighted
R5840 T20433 T20431 compound cell,lines
R5841 T20435 T20431 acl expressing,lines
R5842 T20436 T20435 dobj vector,expressing
R5843 T20437 T20436 amod alone,vector
R5844 T20438 T20436 punct ", ",vector
R5845 T20439 T20440 amod wild,type
R5846 T20440 T20442 compound type,AQP2
R5847 T20441 T20440 punct -,type
R5848 T20442 T20436 conj AQP2,vector
R5849 T20443 T20442 punct ", ",AQP2
R585 T2814 T2813 prep in,resulting
R5850 T20444 T20442 cc or,AQP2
R5851 T20445 T20446 compound AQP2,F204V
R5852 T20446 T20442 conj F204V,AQP2
R5853 T20447 T20446 punct -,F204V
R5854 T20448 T20435 punct (,expressing
R5855 T20449 T20435 cc and,expressing
R5856 T20450 T20451 prep in,expressing
R5857 T20451 T20435 conj expressing,expressing
R5858 T20452 T20453 det some,cases
R5859 T20453 T20450 pobj cases,in
R586 T2815 T2816 amod severe,NDI
R5860 T20454 T20451 advmod transiently,expressing
R5861 T20455 T20456 det a,construct
R5862 T20456 T20451 dobj construct,expressing
R5863 T20457 T20456 compound GFP,construct
R5864 T20458 T20451 punct ),expressing
R5865 T20459 T20434 auxpass were,grown
R5866 T20460 T20434 prep on,grown
R5867 T20461 T20462 npadvmod fibronectin,coated
R5868 T20462 T20464 amod coated,coverslips
R5869 T20463 T20462 punct -,coated
R587 T2816 T2814 pobj NDI,in
R5870 T20464 T20460 pobj coverslips,on
R5871 T20465 T20466 mark until,formed
R5872 T20466 T20434 advcl formed,grown
R5873 T20467 T20468 amod tight,junctions
R5874 T20468 T20466 nsubj junctions,formed
R5875 T20469 T20434 punct .,grown
R5876 T20471 T20472 nsubjpass Cells,treated
R5877 T20473 T20472 auxpass were,treated
R5878 T20474 T20472 prep with,treated
R5879 T20475 T20474 cc or,with
R588 T2817 T2813 prep in,resulting
R5880 T20476 T20474 conj without,with
R5881 T20477 T20478 nummod 150,μM
R5882 T20478 T20479 compound μM,forskolin
R5883 T20479 T20476 pobj forskolin,without
R5884 T20480 T20472 prep for,treated
R5885 T20481 T20482 nummod 90,min
R5886 T20482 T20480 pobj min,for
R5887 T20483 T20472 punct ", ",treated
R5888 T20484 T20472 cc and,treated
R5889 T20485 T20472 conj fixed,treated
R589 T2818 T2817 pobj humans,in
R5890 T20486 T20485 prep in,fixed
R5891 T20487 T20486 pobj methanol,in
R5892 T20488 T20485 prep at,fixed
R5893 T20489 T20490 punct −,20
R5894 T20490 T20491 nummod 20,°C
R5895 T20491 T20488 pobj °C,at
R5896 T20492 T20472 punct .,treated
R5897 T20494 T20495 advmod Subsequently,washed
R5898 T20496 T20495 punct ", ",washed
R5899 T20497 T20495 nsubj cells,washed
R590 T2819 T2820 punct [,10
R5900 T20498 T20495 aux were,washed
R5901 T20499 T20495 cc and,washed
R5902 T20500 T20495 conj permeabilized,washed
R5903 T20501 T20500 prep in,permeabilized
R5904 T20502 T20503 nummod 0.2,%
R5905 T20503 T20504 compound %,X
R5906 T20504 T20501 pobj X,in
R5907 T20505 T20504 compound Triton,X
R5908 T20506 T20504 punct -,X
R5909 T20507 T20504 nummod 100,X
R591 T2820 T2801 parataxis 10,highlighted
R5910 T20508 T20500 prep for,permeabilized
R5911 T20509 T20510 nummod 5,min
R5912 T20510 T20508 pobj min,for
R5913 T20511 T20495 punct ", ",washed
R5914 T20512 T20495 cc and,washed
R5915 T20513 T20514 advmod sequentially,probed
R5916 T20514 T20495 conj probed,washed
R5917 T20515 T20514 prep for,probed
R5918 T20516 T20515 pobj AQP2,for
R5919 T20517 T20516 cc and,AQP2
R592 T2821 T2820 nummod 3,10
R5920 T20518 T20519 compound organelle,markers
R5921 T20519 T20516 conj markers,AQP2
R5922 T20520 T20519 prep for,markers
R5923 T20521 T20522 preconj either,PM
R5924 T20522 T20520 pobj PM,for
R5925 T20523 T20522 det the,PM
R5926 T20524 T20522 cc or,PM
R5927 T20525 T20526 det the,ER
R5928 T20526 T20522 conj ER,PM
R5929 T20527 T20495 punct .,washed
R593 T2822 T2820 punct ",",10
R5930 T20529 T20530 nsubjpass AQP2,detected
R5931 T20531 T20530 auxpass was,detected
R5932 T20532 T20530 advcl using,detected
R5933 T20533 T20532 dobj goat,using
R5934 T20534 T20533 amod anti-AQP2,goat
R5935 T20535 T20536 punct (,sc
R5936 T20536 T20533 parataxis sc,goat
R5937 T20537 T20536 dep 1,sc
R5938 T20538 T20539 punct :,100
R5939 T20539 T20537 prep 100,1
R594 T2823 T2820 punct ],10
R5940 T20540 T20536 punct ;,sc
R5941 T20541 T20542 compound Santa,Cruz
R5942 T20542 T20543 compound Cruz,Biotechnology
R5943 T20543 T20536 dep Biotechnology,sc
R5944 T20544 T20536 punct ;,sc
R5945 T20545 T20536 punct #,sc
R5946 T20546 T20536 punct -,sc
R5947 T20547 T20536 nummod 9882,sc
R5948 T20548 T20536 punct ),sc
R5949 T20549 T20533 cc and,goat
R595 T2824 T2801 punct .,highlighted
R5950 T20550 T20551 det a,dilution
R5951 T20551 T20533 conj dilution,goat
R5952 T20552 T20553 quantmod 1,200
R5953 T20553 T20551 nummod 200,dilution
R5954 T20554 T20553 punct :,200
R5955 T20555 T20551 prep of,dilution
R5956 T20556 T20557 npadvmod AlexaFluor,conjugated
R5957 T20557 T20560 amod conjugated,antibody
R5958 T20558 T20556 nummod 488,AlexaFluor
R5959 T20559 T20557 punct -,conjugated
R596 T2826 T2827 amod Recessive,mutations
R5960 T20560 T20555 pobj antibody,of
R5961 T20561 T20560 nmod donkey,antibody
R5962 T20562 T20560 amod anti-goat,antibody
R5963 T20563 T20560 amod secondary,antibody
R5964 T20564 T20530 punct .,detected
R5965 T20566 T20567 det The,PM
R5966 T20567 T20568 nsubjpass PM,probed
R5967 T20569 T20567 cc and,PM
R5968 T20570 T20567 conj ER,PM
R5969 T20571 T20568 auxpass were,probed
R597 T2827 T2829 nsubjpass mutations,thought
R5970 T20572 T20568 advcl using,probed
R5971 T20573 T20574 nmod mouse,ATPase
R5972 T20574 T20579 nmod ATPase,antibodies
R5973 T20575 T20574 amod anti-Na+,ATPase
R5974 T20576 T20574 punct /,ATPase
R5975 T20577 T20574 nmod K+,ATPase
R5976 T20578 T20574 punct -,ATPase
R5977 T20579 T20572 dobj antibodies,using
R5978 T20580 T20581 punct (,Upstate
R5979 T20581 T20574 parataxis Upstate,ATPase
R598 T2828 T2827 compound Aqp2,mutations
R5980 T20582 T20581 punct ", ",Upstate
R5981 T20583 T20581 npadvmod Waltham,Upstate
R5982 T20584 T20581 punct ", ",Upstate
R5983 T20585 T20581 npadvmod Massachusetts,Upstate
R5984 T20586 T20581 punct ", ",Upstate
R5985 T20587 T20588 compound United,States
R5986 T20588 T20581 npadvmod States,Upstate
R5987 T20589 T20581 punct ),Upstate
R5988 T20590 T20574 cc or,ATPase
R5989 T20591 T20592 npadvmod rabbit,anti-calnexin
R599 T2830 T2829 auxpass are,thought
R5990 T20592 T20574 conj anti-calnexin,ATPase
R5991 T20593 T20594 punct (,Biotechnology
R5992 T20594 T20592 parataxis Biotechnology,anti-calnexin
R5993 T20595 T20594 compound Stressgen,Biotechnology
R5994 T20596 T20594 punct ", ",Biotechnology
R5995 T20597 T20594 npadvmod Victoria,Biotechnology
R5996 T20598 T20594 punct ", ",Biotechnology
R5997 T20599 T20600 compound British,Columbia
R5998 T20600 T20594 npadvmod Columbia,Biotechnology
R5999 T20601 T20594 punct ", ",Biotechnology
R600 T2831 T2829 advmod generally,thought
R6000 T20602 T20594 npadvmod Canada,Biotechnology
R6001 T20603 T20594 punct ),Biotechnology
R6002 T20604 T20579 cc and,antibodies
R6003 T20605 T20606 det the,antibodies
R6004 T20606 T20579 conj antibodies,antibodies
R6005 T20607 T20606 amod secondary,antibodies
R6006 T20608 T20606 punct ", ",antibodies
R6007 T20609 T20610 npadvmod Cy3,conjugated
R6008 T20610 T20612 amod conjugated,goat
R6009 T20611 T20610 punct -,conjugated
R601 T2832 T2833 aux to,produce
R6010 T20612 T20606 appos goat,antibodies
R6011 T20613 T20612 amod anti-mouse,goat
R6012 T20614 T20615 punct (,ImmunoResearch
R6013 T20615 T20612 parataxis ImmunoResearch,goat
R6014 T20616 T20615 dep 1,ImmunoResearch
R6015 T20617 T20618 punct :,200
R6016 T20618 T20616 prep 200,1
R6017 T20619 T20615 punct ;,ImmunoResearch
R6018 T20620 T20615 compound Jackson,ImmunoResearch
R6019 T20621 T20615 punct ", ",ImmunoResearch
R602 T2833 T2829 xcomp produce,thought
R6020 T20622 T20623 compound West,Grove
R6021 T20623 T20615 npadvmod Grove,ImmunoResearch
R6022 T20624 T20615 punct ", ",ImmunoResearch
R6023 T20625 T20615 npadvmod Pennsylvania,ImmunoResearch
R6024 T20626 T20615 punct ", ",ImmunoResearch
R6025 T20627 T20628 compound United,States
R6026 T20628 T20615 npadvmod States,ImmunoResearch
R6027 T20629 T20615 punct ),ImmunoResearch
R6028 T20630 T20606 cc or,antibodies
R6029 T20631 T20632 npadvmod AlexaFluor,conjugated
R603 T2834 T2835 det an,pore
R6030 T20632 T20635 amod conjugated,chicken
R6031 T20633 T20631 nummod 594,AlexaFluor
R6032 T20634 T20632 punct -,conjugated
R6033 T20635 T20606 conj chicken,antibodies
R6034 T20636 T20635 amod anti-rabbit,chicken
R6035 T20637 T20638 punct (,1
R6036 T20638 T20635 parataxis 1,chicken
R6037 T20639 T20640 punct :,200
R6038 T20640 T20638 prep 200,1
R6039 T20641 T20638 punct ),1
R604 T2835 T2833 dobj pore,produce
R6040 T20642 T20572 advmod respectively,using
R6041 T20643 T20568 punct .,probed
R6042 T20645 T20646 nsubjpass Cells,washed
R6043 T20647 T20646 auxpass were,washed
R6044 T20648 T20646 prep in,washed
R6045 T20649 T20648 pobj PBS,in
R6046 T20650 T20646 punct ", ",washed
R6047 T20651 T20646 conj counterstained,washed
R6048 T20652 T20651 prep with,counterstained
R6049 T20653 T20652 pobj DAPI,with
R605 T2836 T2837 advmod abnormally,localized
R6050 T20654 T20651 punct ", ",counterstained
R6051 T20655 T20651 cc and,counterstained
R6052 T20656 T20651 conj mounted,counterstained
R6053 T20657 T20656 prep in,mounted
R6054 T20658 T20657 pobj Vectashield,in
R6055 T20659 T20646 punct .,washed
R6056 T20661 T20662 prep In,probed
R6057 T20663 T20661 pobj experiments,In
R6058 T20664 T20665 prep in,used
R6059 T20665 T20663 relcl used,experiments
R606 T2837 T2835 amod localized,pore
R6060 T20666 T20664 pobj which,in
R6061 T20667 T20668 compound GFP,fusions
R6062 T20668 T20665 nsubjpass fusions,used
R6063 T20669 T20665 auxpass were,used
R6064 T20670 T20662 punct ", ",probed
R6065 T20671 T20662 nsubjpass AQP2,probed
R6066 T20672 T20662 auxpass was,probed
R6067 T20673 T20662 advcl using,probed
R6068 T20674 T20675 det the,combination
R6069 T20675 T20673 dobj combination,using
R607 T2838 T2837 cc and,localized
R6070 T20676 T20675 compound antibody,combination
R6071 T20677 T20675 acl used,combination
R6072 T20678 T20677 prep for,used
R6073 T20679 T20680 compound kidney,immunohistochemistry
R6074 T20680 T20678 pobj immunohistochemistry,for
R6075 T20681 T20662 prep in,probed
R6076 T20682 T20681 pobj order,in
R6077 T20683 T20684 aux to,detect
R6078 T20684 T20682 acl detect,order
R6079 T20685 T20686 det the,AQP2
R608 T2839 T2840 punct ", ",misfolded
R6080 T20686 T20684 dobj AQP2,detect
R6081 T20687 T20684 prep at,detect
R6082 T20688 T20689 nummod 594,nm
R6083 T20689 T20687 pobj nm,at
R6084 T20690 T20684 punct ", ",detect
R6085 T20691 T20692 aux to,distinguish
R6086 T20692 T20684 advcl distinguish,detect
R6087 T20693 T20692 prep between,distinguish
R6088 T20694 T20695 det the,proteins
R6089 T20695 T20693 pobj proteins,between
R609 T2840 T2837 conj misfolded,localized
R6090 T20696 T20695 compound GFP,proteins
R6091 T20697 T20695 compound fusion,proteins
R6092 T20698 T20662 punct .,probed
R6093 T20832 T20833 amod Confocal,microscopy
R6094 T20834 T20833 punct .,microscopy
R6095 T20836 T20837 amod Optical,images
R6096 T20837 T20841 nsubjpass images,collected
R6097 T20838 T20839 compound z,section
R6098 T20839 T20837 compound section,images
R6099 T20840 T20839 punct -,section
R610 T2841 T2840 prep in,misfolded
R6100 T20842 T20841 auxpass were,collected
R6101 T20843 T20841 prep on,collected
R6102 T20844 T20845 det a,Microscope
R6103 T20845 T20843 pobj Microscope,on
R6104 T20846 T20845 nmod BioRad,Microscope
R6105 T20847 T20848 punct (,Hercules
R6106 T20848 T20846 parataxis Hercules,BioRad
R6107 T20849 T20848 punct ", ",Hercules
R6108 T20850 T20848 npadvmod California,Hercules
R6109 T20851 T20848 punct ", ",Hercules
R611 T2842 T2843 amod most,instances
R6110 T20852 T20853 compound United,States
R6111 T20853 T20848 npadvmod States,Hercules
R6112 T20854 T20848 punct ),Hercules
R6113 T20855 T20856 nmod Rainbow,Radiance
R6114 T20856 T20845 nmod Radiance,Microscope
R6115 T20857 T20856 nummod 2100,Radiance
R6116 T20858 T20859 compound Laser,Scanning
R6117 T20859 T20845 compound Scanning,Microscope
R6118 T20860 T20845 compound Confocal,Microscope
R6119 T20861 T20841 punct .,collected
R612 T2843 T2841 pobj instances,in
R6120 T20863 T20864 compound Image,stacks
R6121 T20864 T20865 nsubjpass stacks,flattened
R6122 T20866 T20865 auxpass were,flattened
R6123 T20867 T20865 punct ", ",flattened
R6124 T20868 T20865 cc or,flattened
R6125 T20869 T20865 conj sectioned,flattened
R6126 T20870 T20869 prep along,sectioned
R6127 T20871 T20872 det the,axis
R6128 T20872 T20870 pobj axis,along
R6129 T20873 T20872 compound z,axis
R613 T2844 T2840 punct ", ",misfolded
R6130 T20874 T20872 punct -,axis
R6131 T20875 T20865 punct ", ",flattened
R6132 T20876 T20877 advmod then,processed
R6133 T20877 T20865 dep processed,flattened
R6134 T20878 T20877 advmod further,processed
R6135 T20879 T20877 advcl using,processed
R6136 T20880 T20881 nmod BioRad,software
R6137 T20881 T20879 dobj software,using
R6138 T20882 T20883 nmod Laser,Sharp
R6139 T20883 T20881 nmod Sharp,software
R614 T2845 T2835 compound water,pore
R6140 T20884 T20883 nummod 2000,Sharp
R6141 T20885 T20881 cc and,software
R6142 T20886 T20887 compound Image,J
R6143 T20887 T20888 compound J,software
R6144 T20888 T20881 conj software,software
R6145 T20889 T20890 punct (,Institutes
R6146 T20890 T20888 parataxis Institutes,software
R6147 T20891 T20890 dep v.,Institutes
R6148 T20892 T20891 nummod 1.32,v.
R6149 T20893 T20890 punct ;,Institutes
R615 T2846 T2847 dep that,responds
R6150 T20894 T20890 compound National,Institutes
R6151 T20895 T20890 prep of,Institutes
R6152 T20896 T20895 pobj Health,of
R6153 T20897 T20890 punct ),Institutes
R6154 T20898 T20865 punct .,flattened
R6155 T20900 T20901 nsubjpass Colocalization,performed
R6156 T20902 T20901 auxpass was,performed
R6157 T20903 T20901 advcl using,performed
R6158 T20904 T20905 det the,coefficient
R6159 T20905 T20903 dobj coefficient,using
R616 T2847 T2835 relcl responds,pore
R6160 T20906 T20905 amod overlay,coefficient
R6161 T20907 T20905 prep of,coefficient
R6162 T20908 T20909 compound Image,J
R6163 T20909 T20910 compound J,software
R6164 T20910 T20907 pobj software,of
R6165 T20911 T20901 punct .,performed
R617 T2848 T2847 advmod abnormally,responds
R618 T2849 T2847 prep to,responds
R619 T2850 T2851 det an,increase
R620 T2851 T2849 pobj increase,to
R621 T2852 T2851 prep in,increase
R622 T2853 T2852 pobj cAMP,in
R623 T2854 T2855 punct [,11
R624 T2855 T2829 parataxis 11,thought
R625 T2856 T2855 nummod 6,11
R626 T2857 T2855 punct ",",11
R627 T2858 T2855 punct ],11
R628 T2859 T2829 punct .,thought
R629 T2861 T2862 advmod Furthermore,described
R630 T2863 T2862 punct ", ",described
R631 T2864 T2865 amod dominant,mutations
R632 T2865 T2862 nsubjpass mutations,described
R633 T2866 T2862 aux have,described
R634 T2867 T2862 auxpass been,described
R635 T2868 T2862 cc and,described
R636 T2869 T2862 conj found,described
R637 T2870 T2871 aux to,misroute
R638 T2871 T2869 xcomp misroute,found
R639 T2872 T2873 preconj both,mutant
R640 T2873 T2871 dobj mutant,misroute
R641 T2874 T2873 det the,mutant
R642 T2875 T2873 cc and,mutant
R643 T2876 T2877 det the,type
R644 T2877 T2880 compound type,protein
R645 T2878 T2877 amod wild,type
R646 T2879 T2877 punct -,type
R647 T2880 T2873 conj protein,mutant
R648 T2881 T2871 prep to,misroute
R649 T2882 T2883 det the,membrane
R650 T2883 T2881 pobj membrane,to
R651 T2884 T2883 amod basolateral,membrane
R652 T2885 T2886 punct [,12
R653 T2886 T2869 parataxis 12,found
R654 T2887 T2886 nummod 6,12
R655 T2888 T2886 punct ",",12
R656 T2889 T2886 punct ],12
R657 T2890 T2862 punct .,described
R658 T2892 T2893 amod Several,models
R659 T2893 T2895 nsubjpass models,generated
R660 T2894 T2893 compound mouse,models
R661 T2896 T2893 prep of,models
R662 T2897 T2898 compound diabetes,insipidus
R663 T2898 T2896 pobj insipidus,of
R664 T2899 T2895 aux have,generated
R665 T2900 T2895 auxpass been,generated
R666 T2901 T2902 punct [,13
R667 T2902 T2895 parataxis 13,generated
R668 T2903 T2904 punct –,17
R669 T2904 T2902 prep 17,13
R670 T2905 T2902 punct ],13
R671 T2906 T2895 punct .,generated
R672 T2908 T2909 prep In,generated
R673 T2910 T2911 det an,attempt
R674 T2911 T2908 pobj attempt,In
R675 T2912 T2913 aux to,recapitulate
R676 T2913 T2911 acl recapitulate,attempt
R677 T2914 T2915 amod human,NDI
R678 T2915 T2913 dobj NDI,recapitulate
R679 T2916 T2909 punct ", ",generated
R680 T2917 T2909 nsubjpass mice,generated
R681 T2918 T2909 aux have,generated
R682 T2919 T2909 auxpass been,generated
R683 T2920 T2909 prep with,generated
R684 T2921 T2920 pobj mutations,with
R685 T2922 T2921 prep in,mutations
R686 T2923 T2922 pobj Aqp2,in
R687 T2924 T2923 cc and,Aqp2
R688 T2925 T2923 conj Avpr2,Aqp2
R689 T2926 T2927 punct [,18
R690 T2927 T2909 parataxis 18,generated
R691 T2928 T2927 nummod 15,18
R692 T2929 T2927 punct ",",18
R693 T2930 T2927 punct ],18
R694 T2931 T2909 punct .,generated
R695 T2933 T2934 nsubj Yang,created
R696 T2935 T2933 cc and,Yang
R697 T2936 T2933 conj colleagues,Yang
R698 T2937 T2938 det a,mouse
R699 T2938 T2934 dobj mouse,created
R700 T2939 T2938 prep with,mouse
R701 T2940 T2941 det a,mutation
R702 T2941 T2939 pobj mutation,with
R703 T2942 T2941 nmod T126M,mutation
R704 T2943 T2941 amod knock,mutation
R705 T2944 T2943 punct -,knock
R706 T2945 T2943 prt in,knock
R707 T2946 T2941 prep in,mutation
R708 T2947 T2948 det the,gene
R709 T2948 T2946 pobj gene,in
R710 T2949 T2948 compound Aqp2,gene
R711 T2950 T2934 punct .,created
R712 T2952 T2953 advmod Unexpectedly,died
R713 T2954 T2953 punct ", ",died
R714 T2955 T2956 amod homozygous,mice
R715 T2956 T2953 nsubj mice,died
R716 T2957 T2956 compound mutant,mice
R717 T2958 T2953 prep within,died
R718 T2959 T2960 nummod 6,d
R719 T2960 T2958 pobj d,within
R720 T2961 T2960 prep after,d
R721 T2962 T2961 pobj birth,after
R722 T2963 T2953 punct .,died
R723 T2965 T2966 advmod Interestingly,die
R724 T2967 T2966 punct ", ",die
R725 T2968 T2969 npadvmod AVPR2,deficient
R726 T2969 T2971 amod deficient,pups
R727 T2970 T2969 punct -,deficient
R728 T2971 T2966 nsubj pups,die
R729 T2972 T2971 amod male,pups
R730 T2973 T2966 advmod also,die
R731 T2974 T2966 prep within,die
R732 T2975 T2976 det the,week
R733 T2976 T2974 pobj week,within
R734 T2977 T2976 amod first,week
R735 T2978 T2976 prep after,week
R736 T2979 T2978 pobj birth,after
R737 T2980 T2966 punct .,die
R738 T2982 T2983 advmod Together,suggest
R739 T2984 T2985 det these,models
R740 T2985 T2983 nsubj models,suggest
R741 T2986 T2987 mark that,be
R742 T2987 T2983 ccomp be,suggest
R743 T2988 T2989 det the,mouse
R744 T2989 T2987 nsubj mouse,be
R745 T3096 T3095 punct -,duct
R746 T2990 T2987 aux may,be
R747 T3097 T3084 punct ", ",adopts
R748 T2991 T2992 det a,organism
R749 T2992 T2987 attr organism,be
R750 T3098 T3084 cc and,adopts
R751 T2993 T2994 advmod highly,sensitive
R752 T2994 T2992 amod sensitive,organism
R753 T3099 T3084 conj was,adopts
R754 T2995 T2987 prep with,be
R755 T2996 T2995 pobj regard,with
R756 T3100 T3099 acomp resistant,was
R757 T2997 T2996 prep to,regard
R758 T2998 T2999 compound water,homeostasis
R759 T2999 T2997 pobj homeostasis,to
R760 T3000 T2987 punct ", ",be
R761 T3001 T2987 cc and,be
R762 T3101 T3100 prep to,resistant
R763 T3002 T2987 conj is,be
R764 T3003 T3002 acomp unable,is
R765 T3004 T3005 aux to,survive
R766 T3102 T3101 pobj translocation,to
R767 T3005 T3003 xcomp survive,unable
R768 T3006 T3005 prep with,survive
R769 T3007 T3006 pobj polyuria,with
R770 T3103 T3102 acl induced,translocation
R771 T3008 T2983 punct .,suggest
R772 T3010 T3011 prep In,identified
R773 T3104 T3103 agent by,induced
R774 T3012 T3013 det a,screen
R775 T3105 T3104 pobj desmopressin,by
R776 T3013 T3010 pobj screen,In
R777 T3014 T3015 advmod forward,genetic
R778 T3015 T3013 amod genetic,screen
R779 T3016 T3011 punct ", ",identified
R780 T3106 T3105 punct ", ",desmopressin
R781 T3017 T3018 det a,mouse
R782 T3018 T3011 nsubjpass mouse,identified
R783 T3107 T3108 det an,agonist
R784 T3019 T3018 prep with,mouse
R785 T3020 T3021 det an,mutation
R786 T3021 T3019 pobj mutation,with
R787 T3108 T3105 appos agonist,desmopressin
R788 T3109 T3108 prep of,agonist
R789 T3022 T3021 compound Aqp2,mutation
R790 T3023 T3011 auxpass was,identified
R791 T3024 T3011 punct .,identified
R792 T3026 T3027 det The,purpose
R793 T3110 T3109 pobj AVP,of
R794 T3027 T3028 nsubj purpose,was
R795 T3029 T3027 prep of,purpose
R796 T3030 T3031 det this,study
R797 T3111 T3082 punct .,concluded
R798 T3031 T3029 pobj study,of
R799 T3032 T3033 aux to,characterize
R800 T3033 T3028 xcomp characterize,was
R801 T3113 T3114 advmod In,vitro
R802 T3034 T3035 det this,model
R803 T3035 T3033 dobj model,characterize
R804 T3036 T3035 amod murine,model
R805 T3037 T3035 prep of,model
R806 T3114 T3115 amod vitro,studies
R807 T3038 T3039 amod recessive,DI
R808 T3039 T3037 pobj DI,of
R809 T3115 T3116 nsubj studies,demonstrated
R810 T3040 T3039 amod nephrogenic,DI
R811 T3041 T3028 punct .,was
R812 T3043 T3044 nsubj We,report
R813 T3045 T3044 advmod now,report
R814 T3117 T3115 acl using,studies
R815 T3046 T3047 det a,mutation
R816 T3047 T3044 dobj mutation,report
R817 T3048 T3047 amod novel,mutation
R818 T3049 T3047 compound F204V,mutation
R819 T3118 T3119 det the,line
R820 T3050 T3047 prep in,mutation
R821 T3051 T3052 det the,gene
R822 T3052 T3050 pobj gene,in
R823 T3119 T3117 dobj line,using
R824 T3053 T3052 compound Aqp2,gene
R825 T3054 T3044 punct .,report
R826 T3120 T3121 nmod Madin,Darby
R827 T3056 T3057 det This,allele
R828 T3057 T3058 nsubjpass allele,found
R829 T3121 T3123 nmod Darby,kidney
R830 T3059 T3057 prep of,allele
R831 T3060 T3059 pobj Aqp2,of
R832 T3122 T3121 punct -,Darby
R833 T3061 T3058 auxpass was,found
R834 T3062 T3063 aux to,cause
R835 T3063 T3058 xcomp cause,found
R836 T3123 T3119 nmod kidney,line
R837 T3064 T3065 det the,model
R838 T3065 T3068 nsubj model,survive
R839 T3124 T3123 amod canine,kidney
R840 T3066 T3065 amod first,model
R841 T3067 T3065 compound mouse,model
R842 T3125 T3123 punct (,kidney
R843 T3068 T3063 ccomp survive,cause
R844 T3069 T3065 prep of,model
R845 T3070 T3069 pobj NDI,of
R846 T3126 T3123 appos MDCK,kidney
R847 T3071 T3068 aux to,survive
R848 T3072 T3068 prep past,survive
R849 T3073 T3074 det the,week
R850 T3127 T3119 punct ),line
R851 T3074 T3072 pobj week,past
R852 T3075 T3074 amod first,week
R853 T3076 T3074 prep of,week
R854 T3128 T3119 compound cell,line
R855 T3129 T3130 det an,pattern
R856 T3130 T3116 dobj pattern,demonstrated
R857 T3077 T3076 pobj life,of
R858 T3078 T3058 punct .,found
R859 T3131 T3130 amod endoplasmic,pattern
R860 T3080 T3081 amod Molecular,analyses
R861 T3132 T3130 compound reticulum,pattern
R862 T3081 T3082 nsubj analyses,concluded
R863 T3083 T3084 mark that,adopts
R864 T3084 T3082 ccomp adopts,concluded
R865 T3085 T3086 compound mutant,AQP2
R866 T3086 T3084 nsubj AQP2,adopts
R867 T3133 T3130 prep for,pattern
R868 T3087 T3088 det a,localization
R869 T3088 T3084 dobj localization,adopts
R870 T3089 T3088 amod different,localization
R871 T3134 T3135 det the,protein
R872 T3090 T3088 amod subcellular,localization
R873 T3091 T3084 prep in,adopts
R874 T3092 T3093 amod renal,cells
R875 T3135 T3133 pobj protein,for
R876 T3093 T3091 pobj cells,in
R877 T3094 T3095 amod collecting,duct
R878 T3136 T3135 compound mutant,protein
R879 T3095 T3093 compound duct,cells
R880 T3137 T3130 punct ", ",pattern
R881 T3138 T3130 cc and,pattern
R882 T3139 T3140 amod apparent,resistance
R883 T3140 T3130 conj resistance,pattern
R884 T3141 T3140 prep to,resistance
R885 T3142 T3141 pobj translocation,to
R886 T3143 T3116 punct .,demonstrated
R887 T3145 T3146 det These,data
R888 T3146 T3147 nsubj data,prove
R889 T3148 T3147 advmod conclusively,prove
R890 T3149 T3150 mark that,is
R891 T3150 T3147 ccomp is,prove
R892 T3151 T3152 amod autosomal,NDI
R893 T3152 T3150 nsubj NDI,is
R894 T3153 T3152 amod recessive,NDI
R895 T3154 T3155 det a,consequence
R896 T3155 T3150 attr consequence,is
R897 T3156 T3155 prep of,consequence
R898 T3157 T3158 amod improper,routing
R899 T3158 T3156 pobj routing,of
R900 T3159 T3158 compound AQP2,routing
R901 T3160 T3158 prep in,routing
R902 T3161 T3162 det the,mammal
R903 T3162 T3160 pobj mammal,in
R904 T3163 T3162 amod intact,mammal
R905 T3164 T3147 punct .,prove
R916 T8980 T8981 punct [,20
R917 T8951 T8952 prep In,found
R918 T8953 T8954 det a,screen
R919 T8954 T8951 pobj screen,In
R920 T8955 T8956 amod forward,genetic
R921 T8956 T8954 amod genetic,screen
R922 T8957 T8958 dep that,used
R923 T8958 T8954 relcl used,screen
R924 T8959 T8958 dobj ethylnitrosourea,used
R925 T8960 T8959 punct (,ethylnitrosourea
R926 T8961 T8959 appos ENU,ethylnitrosourea
R927 T8981 T8958 parataxis 20,used
R928 T8962 T8958 punct ),used
R929 T8963 T8964 aux to,induce
R930 T8964 T8958 advcl induce,used
R931 T8982 T8981 nummod 19,20
R932 T8965 T8964 dobj mutations,induce
R933 T8966 T8964 prep in,induce
R934 T8967 T8968 det a,animal
R935 T8983 T8981 punct ",",20
R936 T8968 T8966 pobj animal,in
R937 T8969 T8968 compound founder,animal
R938 T8970 T8971 poss whose,offspring
R939 T8984 T8981 punct ],20
R940 T8971 T8972 dep offspring,screened
R941 T8972 T8968 relcl screened,animal
R942 T8973 T8972 auxpass were,screened
R943 T8985 T8952 punct ", ",found
R944 T8974 T8972 advmod then,screened
R945 T8986 T8952 nsubj we,found
R946 T8975 T8972 prep for,screened
R947 T8976 T8977 amod abnormal,metabolism
R948 T8987 T8988 det a,family
R949 T8977 T8975 pobj metabolism,for
R950 T8978 T8979 amod whole,body
R951 T8988 T8952 dobj family,found
R952 T8979 T8977 compound body,metabolism
R953 T8989 T8988 prep of,family
R954 T8990 T8989 pobj mice,of
R955 T8991 T8992 dep that,urinated
R956 T9086 T9084 pobj substitution,to
R957 T9087 T9086 nmod valine,substitution
R958 T9088 T9087 prep for,valine
R959 T9089 T9088 pobj phenylalanine,for
R960 T9090 T9086 prep at,substitution
R961 T8992 T8988 relcl urinated,family
R962 T9091 T9092 compound amino,acid
R963 T9092 T9090 pobj acid,at
R964 T9093 T9092 nummod 204,acid
R965 T9094 T9092 prep of,acid
R966 T8993 T8992 cc and,urinated
R967 T9095 T9096 det the,protein
R968 T9096 T9094 pobj protein,of
R969 T9097 T9092 punct (,acid
R970 T8994 T8992 conj drank,urinated
R971 T9098 T9092 appos F204V,acid
R972 T9099 T9092 punct ),acid
R973 T9100 T9056 punct .,identified
R974 T8995 T8994 advmod excessively,drank
R975 T8996 T8952 punct .,found
R976 T9102 T9103 nsubj AQP2,is
R977 T9104 T9105 det a,channel
R978 T8998 T8999 nmod Serum,analysis
R979 T9105 T9103 attr channel,is
R980 T9106 T9107 nummod six,transmembrane
R981 T9107 T9105 compound transmembrane,channel
R982 T9108 T9107 punct -,transmembrane
R983 T8999 T9002 nsubj analysis,showed
R984 T9109 T9105 compound water,channel
R985 T9110 T9103 punct ", ",is
R986 T9000 T8998 cc and,Serum
R987 T9111 T9103 cc and,is
R988 T9112 T9113 nsubj F204,lies
R989 T9113 T9103 conj lies,is
R990 T9001 T8998 conj urine,Serum
R991 T9114 T9113 prep near,lies
R992 T9115 T9116 det the,face
R993 T9003 T9004 mark that,were
R994 T9116 T9114 pobj face,near
R995 T9117 T9116 amod extracellular,face
R996 T9118 T9116 prep of,face
R997 T9119 T9120 det the,domain
R998 T9120 T9118 pobj domain,of
R999 T9004 T9002 ccomp were,showed