PMC:1064854 / 65139-66279 JSONTXT 7 Projects

Annnotations TAB TSV DIC JSON TextAE

Id Subject Object Predicate Lexical cue
T40046 0-9 NN denotes Histology
T40047 10-13 CC denotes and
T40048 14-22 NN denotes electron
T40049 23-33 NN denotes microscopy
T40050 33-171 sentence denotes Soleus muscle and liver were dissected and fixed overnight in 2% glutaraldehyde, 1% paraformaldehyde, and 0.08% sodium cacodylate buffer.
T40051 34-40 NN denotes Soleus
T40052 41-47 NN denotes muscle
T40054 48-51 CC denotes and
T40055 52-57 NN denotes liver
T40056 58-62 VBD denotes were
T40053 63-72 VBN denotes dissected
T40057 73-76 CC denotes and
T40058 77-82 VBN denotes fixed
T40059 83-92 RB denotes overnight
T40060 93-95 IN denotes in
T40061 96-97 CD denotes 2
T40062 97-98 NN denotes %
T40063 99-113 NN denotes glutaraldehyde
T40065 113-115 , denotes ,
T40066 115-116 CD denotes 1
T40067 116-117 NN denotes %
T40068 118-134 NN denotes paraformaldehyde
T40069 134-136 , denotes ,
T40070 136-139 CC denotes and
T40071 140-144 CD denotes 0.08
T40072 144-145 NN denotes %
T40073 146-152 NN denotes sodium
T40074 153-163 NN denotes cacodylate
T40064 164-170 NN denotes buffer
T40075 170-171 . denotes .
T40076 171-327 sentence denotes The tissues were postfixed in 1% osmium tetroxide, dehydrated in graded ethanol, embedded in Poly Bed plastic resin, and sectioned for electron microscopy.
T40077 172-175 DT denotes The
T40078 176-183 NNS denotes tissues
T40080 184-188 VBD denotes were
T40079 189-198 VBN denotes postfixed
T40081 199-201 IN denotes in
T40082 202-203 CD denotes 1
T40083 203-204 NN denotes %
T40085 205-211 NN denotes osmium
T40084 212-221 NN denotes tetroxide
T40086 221-223 , denotes ,
T40087 223-233 VBN denotes dehydrated
T40088 234-236 IN denotes in
T40089 237-243 JJ denotes graded
T40090 244-251 NN denotes ethanol
T40091 251-253 , denotes ,
T40092 253-261 VBN denotes embedded
T40093 262-264 IN denotes in
T40094 265-269 NNP denotes Poly
T40095 270-273 NNP denotes Bed
T40097 274-281 NN denotes plastic
T40096 282-287 NN denotes resin
T40098 287-289 , denotes ,
T40099 289-292 CC denotes and
T40100 293-302 VBN denotes sectioned
T40101 303-306 IN denotes for
T40102 307-315 NN denotes electron
T40103 316-326 NN denotes microscopy
T40104 326-327 . denotes .
T40105 327-475 sentence denotes Cardiac and skeletal muscle mitochondrial and myofibrillar volume densities were determined from electron micrographs as described previously [10].
T40106 328-335 JJ denotes Cardiac
T40108 336-339 CC denotes and
T40109 340-348 JJ denotes skeletal
T40107 349-355 NN denotes muscle
T40111 356-369 JJ denotes mitochondrial
T40112 370-373 CC denotes and
T40113 374-386 JJ denotes myofibrillar
T40114 387-393 NN denotes volume
T40110 394-403 NNS denotes densities
T40116 404-408 VBD denotes were
T40115 409-419 VBN denotes determined
T40117 420-424 IN denotes from
T40118 425-433 NN denotes electron
T40119 434-445 NNS denotes micrographs
T40120 446-448 IN denotes as
T40121 449-458 VBN denotes described
T40122 459-469 RB denotes previously
T40123 470-471 -LRB- denotes [
T40124 471-473 CD denotes 10
T40125 473-474 -RRB- denotes ]
T40126 474-475 . denotes .
T40127 475-581 sentence denotes For each animal, three different fields at the magnification of 7500× were quantified in blinded fashion.
T40128 476-479 IN denotes For
T40130 480-484 DT denotes each
T40131 485-491 NN denotes animal
T40132 491-493 , denotes ,
T40133 493-498 CD denotes three
T40135 499-508 JJ denotes different
T40134 509-515 NNS denotes fields
T40136 516-518 IN denotes at
T40137 519-522 DT denotes the
T40138 523-536 NN denotes magnification
T40139 537-539 IN denotes of
T40140 540-544 NN denotes 7500
T40141 544-545 SYM denotes ×
T40142 546-550 VBD denotes were
T40129 551-561 VBN denotes quantified
T40143 562-564 IN denotes in
T40144 565-572 JJ denotes blinded
T40145 573-580 NN denotes fashion
T40146 580-581 . denotes .
T40147 581-669 sentence denotes Data were expressed as mean volume density of mitochondria or myofibrils in each field.
T40148 582-586 NNS denotes Data
T40150 587-591 VBD denotes were
T40149 592-601 VBN denotes expressed
T40151 602-604 IN denotes as
T40152 605-609 NN denotes mean
T40154 610-616 NN denotes volume
T40153 617-624 NN denotes density
T40155 625-627 IN denotes of
T40156 628-640 NNS denotes mitochondria
T40157 641-643 CC denotes or
T40158 644-654 NNS denotes myofibrils
T40159 655-657 IN denotes in
T40160 658-662 DT denotes each
T40161 663-668 NN denotes field
T40162 668-669 . denotes .
T40163 669-767 sentence denotes For electron microscopic analysis of the brain, tissue was prepared as previously described [46].
T40164 670-673 IN denotes For
T40166 674-682 NN denotes electron
T40168 683-694 JJ denotes microscopic
T40167 695-703 NN denotes analysis
T40169 704-706 IN denotes of
T40170 707-710 DT denotes the
T40171 711-716 NN denotes brain
T40172 716-718 , denotes ,
T40173 718-724 NN denotes tissue
T40174 725-728 VBD denotes was
T40165 729-737 VBN denotes prepared
T40175 738-740 IN denotes as
T40177 741-751 RB denotes previously
T40176 752-761 VBN denotes described
T40178 762-763 -LRB- denotes [
T40179 763-765 CD denotes 46
T40180 765-766 -RRB- denotes ]
T40181 766-767 . denotes .
T40182 767-932 sentence denotes Ultrathin sections of cortex were cut onto formvar-coated slot grids stained with uranyl acetate and lead citrate and examined with a JEOL 1200 electron microscope.
T40183 768-777 JJ denotes Ultrathin
T40184 778-786 NNS denotes sections
T40186 787-789 IN denotes of
T40187 790-796 NN denotes cortex
T40188 797-801 VBD denotes were
T40185 802-805 VBN denotes cut
T40189 806-810 IN denotes onto
T40190 811-818 NN denotes formvar
T40192 818-819 HYPH denotes -
T40191 819-825 VBN denotes coated
T40194 826-830 NN denotes slot
T40193 831-836 NNS denotes grids
T40195 837-844 VBN denotes stained
T40196 845-849 IN denotes with
T40197 850-856 NN denotes uranyl
T40198 857-864 NN denotes acetate
T40199 865-868 CC denotes and
T40200 869-873 NN denotes lead
T40201 874-881 NN denotes citrate
T40202 882-885 CC denotes and
T40203 886-894 VBN denotes examined
T40204 895-899 IN denotes with
T40205 900-901 DT denotes a
T40207 902-906 NNP denotes JEOL
T40208 907-911 CD denotes 1200
T40209 912-920 NN denotes electron
T40206 921-931 NN denotes microscope
T40210 931-932 . denotes .
T40211 932-1140 sentence denotes For H&E staining, sections of brain, including cerebral cortex, brainstem, and cerebellum, were dehydrated in graded concentrations of alcohol and embedded in paraffin from which 5-μm sections were prepared.
T40212 933-936 IN denotes For
T40214 937-938 NN denotes H
T40216 938-939 CC denotes &
T40217 939-940 NN denotes E
T40215 941-949 NN denotes staining
T40218 949-951 , denotes ,
T40219 951-959 NNS denotes sections
T40220 960-962 IN denotes of
T40221 963-968 NN denotes brain
T40222 968-970 , denotes ,
T40223 970-979 VBG denotes including
T40224 980-988 JJ denotes cerebral
T40225 989-995 NN denotes cortex
T40226 995-997 , denotes ,
T40227 997-1006 NN denotes brainstem
T40228 1006-1008 , denotes ,
T40229 1008-1011 CC denotes and
T40230 1012-1022 NN denotes cerebellum
T40231 1022-1024 , denotes ,
T40232 1024-1028 VBD denotes were
T40213 1029-1039 VBN denotes dehydrated
T40233 1040-1042 IN denotes in
T40234 1043-1049 VBN denotes graded
T40235 1050-1064 NNS denotes concentrations
T40236 1065-1067 IN denotes of
T40237 1068-1075 NN denotes alcohol
T40238 1076-1079 CC denotes and
T40239 1080-1088 VBN denotes embedded
T40240 1089-1091 IN denotes in
T40241 1092-1100 NN denotes paraffin
T40242 1101-1105 IN denotes from
T40244 1106-1111 WDT denotes which
T40245 1112-1113 CD denotes 5
T40247 1113-1114 HYPH denotes -
T40246 1114-1116 NN denotes μm
T40248 1117-1125 NNS denotes sections
T40249 1126-1130 VBD denotes were
T40243 1131-1139 VBN denotes prepared
T40250 1139-1140 . denotes .
R10200 T40047 T40046 cc and,Histology
R10201 T40048 T40049 compound electron,microscopy
R10202 T40049 T40046 conj microscopy,Histology
R10203 T40051 T40052 compound Soleus,muscle
R10204 T40052 T40053 nsubjpass muscle,dissected
R10205 T40054 T40052 cc and,muscle
R10206 T40055 T40052 conj liver,muscle
R10207 T40056 T40053 auxpass were,dissected
R10208 T40057 T40053 cc and,dissected
R10209 T40058 T40053 conj fixed,dissected
R10210 T40059 T40058 advmod overnight,fixed
R10211 T40060 T40058 prep in,fixed
R10212 T40061 T40062 nummod 2,%
R10213 T40062 T40063 nmod %,glutaraldehyde
R10214 T40063 T40064 nmod glutaraldehyde,buffer
R10215 T40064 T40060 pobj buffer,in
R10216 T40065 T40063 punct ", ",glutaraldehyde
R10217 T40066 T40067 nummod 1,%
R10218 T40067 T40068 compound %,paraformaldehyde
R10219 T40068 T40063 conj paraformaldehyde,glutaraldehyde
R10220 T40069 T40068 punct ", ",paraformaldehyde
R10221 T40070 T40068 cc and,paraformaldehyde
R10222 T40071 T40072 nummod 0.08,%
R10223 T40072 T40068 conj %,paraformaldehyde
R10224 T40073 T40074 compound sodium,cacodylate
R10225 T40074 T40072 appos cacodylate,%
R10226 T40075 T40053 punct .,dissected
R10227 T40077 T40078 det The,tissues
R10228 T40078 T40079 nsubjpass tissues,postfixed
R10229 T40080 T40079 auxpass were,postfixed
R10230 T40081 T40079 prep in,postfixed
R10231 T40082 T40083 nummod 1,%
R10232 T40083 T40084 compound %,tetroxide
R10233 T40084 T40081 pobj tetroxide,in
R10234 T40085 T40084 compound osmium,tetroxide
R10235 T40086 T40079 punct ", ",postfixed
R10236 T40087 T40079 conj dehydrated,postfixed
R10237 T40088 T40087 prep in,dehydrated
R10238 T40089 T40090 amod graded,ethanol
R10239 T40090 T40088 pobj ethanol,in
R10240 T40091 T40087 punct ", ",dehydrated
R10241 T40092 T40087 conj embedded,dehydrated
R10242 T40093 T40092 prep in,embedded
R10243 T40094 T40095 compound Poly,Bed
R10244 T40095 T40096 compound Bed,resin
R10245 T40096 T40093 pobj resin,in
R10246 T40097 T40096 compound plastic,resin
R10247 T40098 T40092 punct ", ",embedded
R10248 T40099 T40092 cc and,embedded
R10249 T40100 T40092 conj sectioned,embedded
R10250 T40101 T40100 prep for,sectioned
R10251 T40102 T40103 compound electron,microscopy
R10252 T40103 T40101 pobj microscopy,for
R10253 T40104 T40079 punct .,postfixed
R10254 T40106 T40107 amod Cardiac,muscle
R10255 T40107 T40110 nmod muscle,densities
R10256 T40108 T40106 cc and,Cardiac
R10257 T40109 T40106 conj skeletal,Cardiac
R10258 T40110 T40115 nsubjpass densities,determined
R10259 T40111 T40110 amod mitochondrial,densities
R10260 T40112 T40111 cc and,mitochondrial
R10261 T40113 T40111 conj myofibrillar,mitochondrial
R10262 T40114 T40110 compound volume,densities
R10263 T40116 T40115 auxpass were,determined
R10264 T40117 T40115 prep from,determined
R10265 T40118 T40119 compound electron,micrographs
R10266 T40119 T40117 pobj micrographs,from
R10267 T40120 T40121 mark as,described
R10268 T40121 T40115 advcl described,determined
R10269 T40122 T40121 advmod previously,described
R10270 T40123 T40124 punct [,10
R10271 T40124 T40121 parataxis 10,described
R10272 T40125 T40124 punct ],10
R10273 T40126 T40115 punct .,determined
R10274 T40128 T40129 prep For,quantified
R10275 T40130 T40131 det each,animal
R10276 T40131 T40128 pobj animal,For
R10277 T40132 T40129 punct ", ",quantified
R10278 T40133 T40134 nummod three,fields
R10279 T40134 T40129 nsubjpass fields,quantified
R10280 T40135 T40134 amod different,fields
R10281 T40136 T40134 prep at,fields
R10282 T40137 T40138 det the,magnification
R10283 T40138 T40136 pobj magnification,at
R10284 T40139 T40138 prep of,magnification
R10285 T40140 T40139 pobj 7500,of
R10286 T40141 T40140 punct ×,7500
R10287 T40142 T40129 auxpass were,quantified
R10288 T40143 T40129 prep in,quantified
R10289 T40144 T40145 amod blinded,fashion
R10290 T40145 T40143 pobj fashion,in
R10291 T40146 T40129 punct .,quantified
R10292 T40148 T40149 nsubjpass Data,expressed
R10293 T40150 T40149 auxpass were,expressed
R10294 T40151 T40149 prep as,expressed
R10295 T40152 T40153 compound mean,density
R10296 T40153 T40151 pobj density,as
R10297 T40154 T40153 compound volume,density
R10298 T40155 T40153 prep of,density
R10299 T40156 T40155 pobj mitochondria,of
R10300 T40157 T40156 cc or,mitochondria
R10301 T40158 T40156 conj myofibrils,mitochondria
R10302 T40159 T40153 prep in,density
R10303 T40160 T40161 det each,field
R10304 T40161 T40159 pobj field,in
R10305 T40162 T40149 punct .,expressed
R10306 T40164 T40165 prep For,prepared
R10307 T40166 T40167 nmod electron,analysis
R10308 T40167 T40164 pobj analysis,For
R10309 T40168 T40167 amod microscopic,analysis
R10310 T40169 T40167 prep of,analysis
R10311 T40170 T40171 det the,brain
R10312 T40171 T40169 pobj brain,of
R10313 T40172 T40165 punct ", ",prepared
R10314 T40173 T40165 nsubjpass tissue,prepared
R10315 T40174 T40165 auxpass was,prepared
R10316 T40175 T40176 mark as,described
R10317 T40176 T40165 advcl described,prepared
R10318 T40177 T40176 advmod previously,described
R10319 T40178 T40179 punct [,46
R10320 T40179 T40176 parataxis 46,described
R10321 T40180 T40179 punct ],46
R10322 T40181 T40165 punct .,prepared
R10323 T40183 T40184 amod Ultrathin,sections
R10324 T40184 T40185 nsubjpass sections,cut
R10325 T40186 T40184 prep of,sections
R10326 T40187 T40186 pobj cortex,of
R10327 T40188 T40185 auxpass were,cut
R10328 T40189 T40185 prep onto,cut
R10329 T40190 T40191 npadvmod formvar,coated
R10330 T40191 T40193 amod coated,grids
R10331 T40192 T40191 punct -,coated
R10332 T40193 T40189 pobj grids,onto
R10333 T40194 T40193 compound slot,grids
R10334 T40195 T40193 acl stained,grids
R10335 T40196 T40195 prep with,stained
R10336 T40197 T40198 compound uranyl,acetate
R10337 T40198 T40196 pobj acetate,with
R10338 T40199 T40198 cc and,acetate
R10339 T40200 T40201 compound lead,citrate
R10340 T40201 T40198 conj citrate,acetate
R10341 T40202 T40185 cc and,cut
R10342 T40203 T40185 conj examined,cut
R10343 T40204 T40203 prep with,examined
R10344 T40205 T40206 det a,microscope
R10345 T40206 T40204 pobj microscope,with
R10346 T40207 T40206 nmod JEOL,microscope
R10347 T40208 T40207 nummod 1200,JEOL
R10348 T40209 T40206 compound electron,microscope
R10349 T40210 T40185 punct .,cut
R10350 T40212 T40213 prep For,dehydrated
R10351 T40214 T40215 nmod H,staining
R10352 T40215 T40212 pobj staining,For
R10353 T40216 T40214 cc &,H
R10354 T40217 T40214 conj E,H
R10355 T40218 T40213 punct ", ",dehydrated
R10356 T40219 T40213 nsubjpass sections,dehydrated
R10357 T40220 T40219 prep of,sections
R10358 T40221 T40220 pobj brain,of
R10359 T40222 T40219 punct ", ",sections
R10360 T40223 T40219 prep including,sections
R10361 T40224 T40225 amod cerebral,cortex
R10362 T40225 T40223 pobj cortex,including
R10363 T40226 T40225 punct ", ",cortex
R10364 T40227 T40225 conj brainstem,cortex
R10365 T40228 T40227 punct ", ",brainstem
R10366 T40229 T40227 cc and,brainstem
R10367 T40230 T40227 conj cerebellum,brainstem
R10368 T40231 T40213 punct ", ",dehydrated
R10369 T40232 T40213 auxpass were,dehydrated
R10370 T40233 T40213 prep in,dehydrated
R10371 T40234 T40235 amod graded,concentrations
R10372 T40235 T40233 pobj concentrations,in
R10373 T40236 T40235 prep of,concentrations
R10374 T40237 T40236 pobj alcohol,of
R10375 T40238 T40213 cc and,dehydrated
R10376 T40239 T40213 conj embedded,dehydrated
R10377 T40240 T40239 prep in,embedded
R10378 T40241 T40240 pobj paraffin,in
R10379 T40242 T40243 prep from,prepared
R10380 T40243 T40241 relcl prepared,paraffin
R10381 T40244 T40242 pobj which,from
R10382 T40245 T40246 nummod 5,μm
R10383 T40246 T40248 compound μm,sections
R10384 T40247 T40246 punct -,μm
R10385 T40248 T40243 nsubjpass sections,prepared
R10386 T40249 T40243 auxpass were,prepared
R10387 T40250 T40213 punct .,dehydrated