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PMC:1064854 / 62680-64119 JSONTXT 7 Projects

Annnotations TAB TSV DIC JSON TextAE

Id Subject Object Predicate Lexical cue
T38198 0-13 JJ denotes Mitochondrial
T38199 14-25 NN denotes respiration
T38200 26-33 NNS denotes studies
T38201 33-195 sentence denotes Mitochondrial respiration was assessed in saponin-skinned soleus fibers with succinate as substrate and in the presence of rotenone as previously described [44].
T38202 34-47 JJ denotes Mitochondrial
T38203 48-59 NN denotes respiration
T38205 60-63 VBD denotes was
T38204 64-72 VBN denotes assessed
T38206 73-75 IN denotes in
T38207 76-83 NN denotes saponin
T38209 83-84 HYPH denotes -
T38208 84-91 VBN denotes skinned
T38211 92-98 NN denotes soleus
T38210 99-105 NNS denotes fibers
T38212 106-110 IN denotes with
T38213 111-120 NN denotes succinate
T38214 121-123 IN denotes as
T38215 124-133 NN denotes substrate
T38216 134-137 CC denotes and
T38217 138-140 IN denotes in
T38218 141-144 DT denotes the
T38219 145-153 NN denotes presence
T38220 154-156 IN denotes of
T38221 157-165 NN denotes rotenone
T38222 166-168 IN denotes as
T38224 169-179 RB denotes previously
T38223 180-189 VBN denotes described
T38225 190-191 -LRB- denotes [
T38226 191-193 CD denotes 44
T38227 193-194 -RRB- denotes ]
T38228 194-195 . denotes .
T38229 195-293 sentence denotes In brief, 3-mo-old female mice were anesthetized with chloral hydrate (400 mg/kg of body weight).
T38230 196-198 IN denotes In
T38232 199-204 JJ denotes brief
T38233 204-206 , denotes ,
T38234 206-207 CD denotes 3
T38236 207-208 HYPH denotes -
T38235 208-210 NN denotes mo
T38238 210-211 HYPH denotes -
T38237 211-214 JJ denotes old
T38240 215-221 JJ denotes female
T38239 222-226 NNS denotes mice
T38241 227-231 VBD denotes were
T38231 232-244 VBN denotes anesthetized
T38242 245-249 IN denotes with
T38243 250-257 JJ denotes chloral
T38244 258-265 NN denotes hydrate
T38245 266-267 -LRB- denotes (
T38247 267-270 CD denotes 400
T38246 271-273 NN denotes mg
T38248 273-274 SYM denotes /
T38249 274-276 NN denotes kg
T38250 277-279 IN denotes of
T38251 280-284 NN denotes body
T38252 285-291 NN denotes weight
T38253 291-292 -RRB- denotes )
T38254 292-293 . denotes .
T38255 293-604 sentence denotes Soleus fibers were separated and then transferred to a buffer (2.77 mM K2Ca-EGTA, 7.23 mM K2EGTA, 6.56 mM MgCl2, 20 mM imidazole, 53.3 mM K-MES, 20 mM taurine, 5.3 mM ATP, 15 mM PCr, 3 mM KH2PO4, 0.5 mM DTT [pH 7.1]) supplemented with 50 μg/ml saponin and permeabilized for 30 min at 4 °C with gentle stirring.
T38256 294-300 NN denotes Soleus
T38257 301-307 NNS denotes fibers
T38259 308-312 VBD denotes were
T38258 313-322 VBN denotes separated
T38260 323-326 CC denotes and
T38261 327-331 RB denotes then
T38262 332-343 VBN denotes transferred
T38263 344-346 IN denotes to
T38264 347-348 DT denotes a
T38265 349-355 NN denotes buffer
T38266 356-357 -LRB- denotes (
T38267 357-361 CD denotes 2.77
T38268 362-364 NN denotes mM
T38270 365-369 NN denotes K2Ca
T38271 369-370 HYPH denotes -
T38269 370-374 NN denotes EGTA
T38272 374-376 , denotes ,
T38273 376-380 CD denotes 7.23
T38274 381-383 NN denotes mM
T38275 384-390 NN denotes K2EGTA
T38276 390-392 , denotes ,
T38277 392-396 CD denotes 6.56
T38278 397-399 NN denotes mM
T38279 400-405 NN denotes MgCl2
T38280 405-407 , denotes ,
T38281 407-409 CD denotes 20
T38282 410-412 NN denotes mM
T38283 413-422 NN denotes imidazole
T38284 422-424 , denotes ,
T38285 424-428 CD denotes 53.3
T38286 429-431 NN denotes mM
T38288 432-433 NN denotes K
T38289 433-434 HYPH denotes -
T38287 434-437 NN denotes MES
T38290 437-439 , denotes ,
T38291 439-441 CD denotes 20
T38292 442-444 NN denotes mM
T38293 445-452 NN denotes taurine
T38294 452-454 , denotes ,
T38295 454-457 CD denotes 5.3
T38296 458-460 NN denotes mM
T38297 461-464 NN denotes ATP
T38298 464-466 , denotes ,
T38299 466-468 CD denotes 15
T38300 469-471 NN denotes mM
T38301 472-475 NN denotes PCr
T38302 475-477 , denotes ,
T38303 477-478 CD denotes 3
T38304 479-481 NN denotes mM
T38305 482-488 NN denotes KH2PO4
T38306 488-490 , denotes ,
T38307 490-493 CD denotes 0.5
T38308 494-496 NN denotes mM
T38309 497-500 NN denotes DTT
T38310 501-502 -LRB- denotes [
T38311 502-504 NN denotes pH
T38312 505-508 CD denotes 7.1
T38313 508-509 -RRB- denotes ]
T38314 509-510 -RRB- denotes )
T38315 511-523 VBN denotes supplemented
T38316 524-528 IN denotes with
T38317 529-531 CD denotes 50
T38318 532-534 NN denotes μg
T38319 534-535 SYM denotes /
T38321 535-537 NN denotes ml
T38320 538-545 NN denotes saponin
T38322 546-549 CC denotes and
T38323 550-563 VBN denotes permeabilized
T38324 564-567 IN denotes for
T38325 568-570 CD denotes 30
T38326 571-574 NN denotes min
T38327 575-577 IN denotes at
T38328 578-579 CD denotes 4
T38329 580-582 NN denotes °C
T38330 583-587 IN denotes with
T38331 588-594 JJ denotes gentle
T38332 595-603 NN denotes stirring
T38333 603-604 . denotes .
T38334 604-795 sentence denotes Fibers were then washed twice for 10 min each (2.77 mM K2Ca-EGTA, 7.23 mM K2EGTA, 1.38 mM MgCl2, 20 mM imidazole, 100 mM K-MES, 20 mM taurine, 3 mM KH2PO4, 0.5 mM DTT, 2 mg/ml BSA [pH 7.1]).
T38335 605-611 NNS denotes Fibers
T38337 612-616 VBD denotes were
T38338 617-621 RB denotes then
T38336 622-628 VBN denotes washed
T38339 629-634 RB denotes twice
T38340 635-638 IN denotes for
T38341 639-641 CD denotes 10
T38342 642-645 NN denotes min
T38343 646-650 RB denotes each
T38344 651-652 -LRB- denotes (
T38346 652-656 CD denotes 2.77
T38347 657-659 NN denotes mM
T38348 660-664 NN denotes K2Ca
T38349 664-665 HYPH denotes -
T38345 665-669 NN denotes EGTA
T38350 669-671 , denotes ,
T38351 671-675 CD denotes 7.23
T38352 676-678 NN denotes mM
T38353 679-685 NN denotes K2EGTA
T38354 685-687 , denotes ,
T38355 687-691 CD denotes 1.38
T38356 692-694 NN denotes mM
T38357 695-700 NN denotes MgCl2
T38358 700-702 , denotes ,
T38359 702-704 CD denotes 20
T38360 705-707 NN denotes mM
T38361 708-717 NN denotes imidazole
T38362 717-719 , denotes ,
T38363 719-722 CD denotes 100
T38364 723-725 NN denotes mM
T38366 726-727 NN denotes K
T38367 727-728 HYPH denotes -
T38365 728-731 NN denotes MES
T38368 731-733 , denotes ,
T38369 733-735 CD denotes 20
T38370 736-738 NN denotes mM
T38371 739-746 NN denotes taurine
T38372 746-748 , denotes ,
T38373 748-749 CD denotes 3
T38374 750-752 NN denotes mM
T38375 753-759 NN denotes KH2PO4
T38376 759-761 , denotes ,
T38377 761-764 CD denotes 0.5
T38378 765-767 NN denotes mM
T38379 768-771 NN denotes DTT
T38380 771-773 , denotes ,
T38381 773-774 CD denotes 2
T38382 775-777 NN denotes mg
T38383 777-778 SYM denotes /
T38385 778-780 NN denotes ml
T38384 781-784 NN denotes BSA
T38386 785-786 -LRB- denotes [
T38387 786-788 NN denotes pH
T38388 789-792 CD denotes 7.1
T38389 792-793 -RRB- denotes ]
T38390 793-794 -RRB- denotes )
T38391 794-795 . denotes .
T38392 795-920 sentence denotes Respiration was measured at 25 °C using an optical probe (Oxygen FOXY Probe, Ocean Optics, Dunedin, Florida, United States).
T38393 796-807 NN denotes Respiration
T38395 808-811 VBD denotes was
T38394 812-820 VBN denotes measured
T38396 821-823 IN denotes at
T38397 824-826 CD denotes 25
T38398 827-829 NN denotes °C
T38399 830-835 VBG denotes using
T38400 836-838 DT denotes an
T38402 839-846 JJ denotes optical
T38401 847-852 NN denotes probe
T38403 853-854 -LRB- denotes (
T38405 854-860 NNP denotes Oxygen
T38406 861-865 NNP denotes FOXY
T38404 866-871 NNP denotes Probe
T38407 871-873 , denotes ,
T38408 873-878 NNP denotes Ocean
T38409 879-885 NNP denotes Optics
T38410 885-887 , denotes ,
T38411 887-894 NNP denotes Dunedin
T38412 894-896 , denotes ,
T38413 896-903 NNP denotes Florida
T38414 903-905 , denotes ,
T38415 905-911 NNP denotes United
T38416 912-918 NNP denotes States
T38417 918-919 -RRB- denotes )
T38418 919-920 . denotes .
T38419 920-1064 sentence denotes Following measurement of basal state 2 respiration, maximal (ADP-stimulated) state 3 respiration was determined by exposing fibers to 1 mM ADP.
T38420 921-930 VBG denotes Following
T38422 931-942 NN denotes measurement
T38423 943-945 IN denotes of
T38424 946-951 JJ denotes basal
T38426 952-957 NN denotes state
T38427 958-959 CD denotes 2
T38425 960-971 NN denotes respiration
T38428 971-973 , denotes ,
T38429 973-980 JJ denotes maximal
T38431 981-982 -LRB- denotes (
T38432 982-985 NN denotes ADP
T38434 985-986 HYPH denotes -
T38433 986-996 VBN denotes stimulated
T38435 996-997 -RRB- denotes )
T38436 998-1003 NN denotes state
T38437 1004-1005 CD denotes 3
T38430 1006-1017 NN denotes respiration
T38438 1018-1021 VBD denotes was
T38421 1022-1032 VBN denotes determined
T38439 1033-1035 IN denotes by
T38440 1036-1044 VBG denotes exposing
T38441 1045-1051 NNS denotes fibers
T38442 1052-1054 IN denotes to
T38443 1055-1056 CD denotes 1
T38444 1057-1059 NN denotes mM
T38445 1060-1063 NN denotes ADP
T38446 1063-1064 . denotes .
T38447 1064-1191 sentence denotes The integrity of the outer mitochondrial membrane was assessed by adding 8 μM exogenous cytochrome c to ADP-stimulated fibers.
T38448 1065-1068 DT denotes The
T38449 1069-1078 NN denotes integrity
T38451 1079-1081 IN denotes of
T38452 1082-1085 DT denotes the
T38454 1086-1091 JJ denotes outer
T38455 1092-1105 JJ denotes mitochondrial
T38453 1106-1114 NN denotes membrane
T38456 1115-1118 VBD denotes was
T38450 1119-1127 VBN denotes assessed
T38457 1128-1130 IN denotes by
T38458 1131-1137 VBG denotes adding
T38459 1138-1139 CD denotes 8
T38460 1140-1142 NN denotes μM
T38462 1143-1152 JJ denotes exogenous
T38463 1153-1163 NN denotes cytochrome
T38461 1164-1165 NN denotes c
T38464 1166-1168 IN denotes to
T38465 1169-1172 NN denotes ADP
T38467 1172-1173 HYPH denotes -
T38466 1173-1183 VBN denotes stimulated
T38468 1184-1190 NNS denotes fibers
T38469 1190-1191 . denotes .
T38470 1191-1281 sentence denotes State 4 respiration (uncoupled) was evaluated following addition of oligomycin (1 μg/ml).
T38471 1192-1197 NN denotes State
T38473 1198-1199 CD denotes 4
T38472 1200-1211 NN denotes respiration
T38475 1212-1213 -LRB- denotes (
T38476 1213-1222 JJ denotes uncoupled
T38477 1222-1223 -RRB- denotes )
T38478 1224-1227 VBD denotes was
T38474 1228-1237 VBN denotes evaluated
T38479 1238-1247 VBG denotes following
T38480 1248-1256 NN denotes addition
T38481 1257-1259 IN denotes of
T38482 1260-1270 NN denotes oligomycin
T38483 1271-1272 -LRB- denotes (
T38485 1272-1273 CD denotes 1
T38484 1274-1276 NN denotes μg
T38486 1276-1277 SYM denotes /
T38487 1277-1279 NN denotes ml
T38488 1279-1280 -RRB- denotes )
T38489 1280-1281 . denotes .
T38490 1281-1377 sentence denotes The solubility of oxygen in the respiration buffer at 25 °C was taken as 246.87 nmol O2 · ml−1.
T38491 1282-1285 DT denotes The
T38492 1286-1296 NN denotes solubility
T38494 1297-1299 IN denotes of
T38495 1300-1306 NN denotes oxygen
T38496 1307-1309 IN denotes in
T38497 1310-1313 DT denotes the
T38499 1314-1325 NN denotes respiration
T38498 1326-1332 NN denotes buffer
T38500 1333-1335 IN denotes at
T38501 1336-1338 CD denotes 25
T38502 1339-1341 NN denotes °C
T38503 1342-1345 VBD denotes was
T38493 1346-1351 VBN denotes taken
T38504 1352-1354 IN denotes as
T38505 1355-1361 CD denotes 246.87
T38506 1362-1366 NN denotes nmol
T38507 1367-1369 NN denotes O2
T38508 1370-1371 SYM denotes ·
T38509 1372-1374 NN denotes ml
T38510 1374-1375 SYM denotes −
T38511 1375-1376 CD denotes 1
T38512 1376-1377 . denotes .
T38513 1377-1439 sentence denotes Respiration rates were expressed as nmol O2 · min−1 · mgdw−1.
T38514 1378-1389 NN denotes Respiration
T38515 1390-1395 NNS denotes rates
T38517 1396-1400 VBD denotes were
T38516 1401-1410 VBN denotes expressed
T38518 1411-1413 IN denotes as
T38519 1414-1418 NN denotes nmol
T38520 1419-1421 NN denotes O2
T38521 1422-1423 SYM denotes ·
T38522 1424-1427 NN denotes min
T38523 1427-1428 SYM denotes −
T38524 1428-1429 CD denotes 1
T38525 1430-1431 SYM denotes ·
T38526 1432-1436 NN denotes mgdw
T38527 1436-1437 SYM denotes −
T38528 1437-1438 CD denotes 1
T38529 1438-1439 . denotes .
R9675 T38198 T38199 amod Mitochondrial,respiration
R9676 T38199 T38200 compound respiration,studies
R9677 T38202 T38203 amod Mitochondrial,respiration
R9678 T38203 T38204 nsubjpass respiration,assessed
R9679 T38205 T38204 auxpass was,assessed
R9680 T38206 T38204 prep in,assessed
R9681 T38207 T38208 npadvmod saponin,skinned
R9682 T38208 T38210 amod skinned,fibers
R9683 T38209 T38208 punct -,skinned
R9684 T38210 T38206 pobj fibers,in
R9685 T38211 T38210 compound soleus,fibers
R9686 T38212 T38204 prep with,assessed
R9687 T38213 T38212 pobj succinate,with
R9688 T38214 T38213 prep as,succinate
R9689 T38215 T38214 pobj substrate,as
R9690 T38216 T38212 cc and,with
R9691 T38217 T38212 conj in,with
R9692 T38218 T38219 det the,presence
R9693 T38219 T38217 pobj presence,in
R9694 T38220 T38219 prep of,presence
R9695 T38221 T38220 pobj rotenone,of
R9696 T38222 T38223 mark as,described
R9697 T38223 T38204 advcl described,assessed
R9698 T38224 T38223 advmod previously,described
R9699 T38225 T38226 punct [,44
R9700 T38226 T38223 parataxis 44,described
R9701 T38227 T38226 punct ],44
R9702 T38228 T38204 punct .,assessed
R9703 T38230 T38231 prep In,anesthetized
R9704 T38232 T38230 amod brief,In
R9705 T38233 T38231 punct ", ",anesthetized
R9706 T38234 T38235 nummod 3,mo
R9707 T38235 T38237 npadvmod mo,old
R9708 T38236 T38235 punct -,mo
R9709 T38237 T38239 amod old,mice
R9710 T38238 T38237 punct -,old
R9711 T38239 T38231 nsubjpass mice,anesthetized
R9712 T38240 T38239 amod female,mice
R9713 T38241 T38231 auxpass were,anesthetized
R9714 T38242 T38231 prep with,anesthetized
R9715 T38243 T38244 amod chloral,hydrate
R9716 T38244 T38242 pobj hydrate,with
R9717 T38245 T38246 punct (,mg
R9718 T38246 T38244 parataxis mg,hydrate
R9719 T38247 T38246 nummod 400,mg
R9720 T38248 T38249 punct /,kg
R9721 T38249 T38246 prep kg,mg
R9722 T38250 T38249 prep of,kg
R9723 T38251 T38252 compound body,weight
R9724 T38252 T38250 pobj weight,of
R9725 T38253 T38246 punct ),mg
R9726 T38254 T38231 punct .,anesthetized
R9727 T38256 T38257 compound Soleus,fibers
R9728 T38257 T38258 nsubjpass fibers,separated
R9729 T38259 T38258 auxpass were,separated
R9730 T38260 T38258 cc and,separated
R9731 T38261 T38262 advmod then,transferred
R9732 T38262 T38258 conj transferred,separated
R9733 T38263 T38262 prep to,transferred
R9734 T38264 T38265 det a,buffer
R9735 T38265 T38263 pobj buffer,to
R9736 T38266 T38265 punct (,buffer
R9737 T38267 T38268 nummod 2.77,mM
R9738 T38268 T38269 compound mM,EGTA
R9739 T38269 T38265 appos EGTA,buffer
R9740 T38270 T38269 compound K2Ca,EGTA
R9741 T38271 T38269 punct -,EGTA
R9742 T38272 T38269 punct ", ",EGTA
R9743 T38273 T38274 nummod 7.23,mM
R9744 T38274 T38275 compound mM,K2EGTA
R9745 T38275 T38269 appos K2EGTA,EGTA
R9746 T38276 T38269 punct ", ",EGTA
R9747 T38277 T38278 nummod 6.56,mM
R9748 T38278 T38279 compound mM,MgCl2
R9749 T38279 T38269 appos MgCl2,EGTA
R9750 T38280 T38269 punct ", ",EGTA
R9751 T38281 T38282 nummod 20,mM
R9752 T38282 T38283 compound mM,imidazole
R9753 T38283 T38269 appos imidazole,EGTA
R9754 T38284 T38269 punct ", ",EGTA
R9755 T38285 T38286 nummod 53.3,mM
R9756 T38286 T38287 compound mM,MES
R9757 T38287 T38269 appos MES,EGTA
R9758 T38288 T38287 compound K,MES
R9759 T38289 T38287 punct -,MES
R9760 T38290 T38269 punct ", ",EGTA
R9761 T38291 T38292 nummod 20,mM
R9762 T38292 T38293 compound mM,taurine
R9763 T38293 T38269 appos taurine,EGTA
R9764 T38294 T38269 punct ", ",EGTA
R9765 T38295 T38296 nummod 5.3,mM
R9766 T38296 T38297 compound mM,ATP
R9767 T38297 T38269 appos ATP,EGTA
R9768 T38298 T38269 punct ", ",EGTA
R9769 T38299 T38300 nummod 15,mM
R9770 T38300 T38301 compound mM,PCr
R9771 T38301 T38269 appos PCr,EGTA
R9772 T38302 T38269 punct ", ",EGTA
R9773 T38303 T38304 nummod 3,mM
R9774 T38304 T38305 compound mM,KH2PO4
R9775 T38305 T38269 appos KH2PO4,EGTA
R9776 T38306 T38269 punct ", ",EGTA
R9777 T38307 T38308 nummod 0.5,mM
R9778 T38308 T38309 compound mM,DTT
R9779 T38309 T38269 appos DTT,EGTA
R9780 T38310 T38311 punct [,pH
R9781 T38311 T38269 parataxis pH,EGTA
R9782 T38312 T38311 nummod 7.1,pH
R9783 T38313 T38311 punct ],pH
R9784 T38314 T38265 punct ),buffer
R9785 T38315 T38265 acl supplemented,buffer
R9786 T38316 T38315 prep with,supplemented
R9787 T38317 T38318 nummod 50,μg
R9788 T38318 T38316 pobj μg,with
R9789 T38319 T38320 punct /,saponin
R9790 T38320 T38318 prep saponin,μg
R9791 T38321 T38320 compound ml,saponin
R9792 T38322 T38315 cc and,supplemented
R9793 T38323 T38315 conj permeabilized,supplemented
R9794 T38324 T38323 prep for,permeabilized
R9795 T38325 T38326 nummod 30,min
R9796 T38326 T38324 pobj min,for
R9797 T38327 T38323 prep at,permeabilized
R9798 T38328 T38329 nummod 4,°C
R9799 T38329 T38327 pobj °C,at
R9800 T38330 T38323 prep with,permeabilized
R9801 T38331 T38332 amod gentle,stirring
R9802 T38332 T38330 pobj stirring,with
R9803 T38333 T38258 punct .,separated
R9804 T38335 T38336 nsubjpass Fibers,washed
R9805 T38337 T38336 auxpass were,washed
R9806 T38338 T38336 advmod then,washed
R9807 T38339 T38336 advmod twice,washed
R9808 T38340 T38336 prep for,washed
R9809 T38341 T38342 nummod 10,min
R9810 T38342 T38340 pobj min,for
R9811 T38343 T38342 advmod each,min
R9812 T38344 T38345 punct (,EGTA
R9813 T38345 T38336 parataxis EGTA,washed
R9814 T38346 T38347 nummod 2.77,mM
R9815 T38347 T38345 compound mM,EGTA
R9816 T38348 T38345 compound K2Ca,EGTA
R9817 T38349 T38345 punct -,EGTA
R9818 T38350 T38345 punct ", ",EGTA
R9819 T38351 T38352 nummod 7.23,mM
R9820 T38352 T38353 compound mM,K2EGTA
R9821 T38353 T38345 appos K2EGTA,EGTA
R9822 T38354 T38345 punct ", ",EGTA
R9823 T38355 T38356 nummod 1.38,mM
R9824 T38356 T38357 compound mM,MgCl2
R9825 T38357 T38345 appos MgCl2,EGTA
R9826 T38358 T38345 punct ", ",EGTA
R9827 T38359 T38360 nummod 20,mM
R9828 T38360 T38361 compound mM,imidazole
R9829 T38361 T38345 appos imidazole,EGTA
R9830 T38362 T38345 punct ", ",EGTA
R9831 T38363 T38364 nummod 100,mM
R9832 T38364 T38365 compound mM,MES
R9833 T38365 T38345 appos MES,EGTA
R9834 T38366 T38365 compound K,MES
R9835 T38367 T38365 punct -,MES
R9836 T38368 T38345 punct ", ",EGTA
R9837 T38369 T38370 nummod 20,mM
R9838 T38370 T38371 compound mM,taurine
R9839 T38371 T38345 appos taurine,EGTA
R9840 T38372 T38345 punct ", ",EGTA
R9841 T38373 T38374 nummod 3,mM
R9842 T38374 T38375 compound mM,KH2PO4
R9843 T38375 T38345 appos KH2PO4,EGTA
R9844 T38376 T38345 punct ", ",EGTA
R9845 T38377 T38378 nummod 0.5,mM
R9846 T38378 T38379 compound mM,DTT
R9847 T38379 T38345 appos DTT,EGTA
R9848 T38380 T38345 punct ", ",EGTA
R9849 T38381 T38382 nummod 2,mg
R9850 T38382 T38345 appos mg,EGTA
R9851 T38383 T38384 punct /,BSA
R9852 T38384 T38382 prep BSA,mg
R9853 T38385 T38384 compound ml,BSA
R9854 T38386 T38387 punct [,pH
R9855 T38387 T38345 parataxis pH,EGTA
R9856 T38388 T38387 nummod 7.1,pH
R9857 T38389 T38387 punct ],pH
R9858 T38390 T38345 punct ),EGTA
R9859 T38391 T38336 punct .,washed
R9860 T38393 T38394 nsubjpass Respiration,measured
R9861 T38395 T38394 auxpass was,measured
R9862 T38396 T38394 prep at,measured
R9863 T38397 T38398 nummod 25,°C
R9864 T38398 T38396 pobj °C,at
R9865 T38399 T38394 advcl using,measured
R9866 T38400 T38401 det an,probe
R9867 T38401 T38399 dobj probe,using
R9868 T38402 T38401 amod optical,probe
R9869 T38403 T38404 punct (,Probe
R9870 T38404 T38401 parataxis Probe,probe
R9871 T38405 T38404 compound Oxygen,Probe
R9872 T38406 T38404 compound FOXY,Probe
R9873 T38407 T38404 punct ", ",Probe
R9874 T38408 T38409 compound Ocean,Optics
R9875 T38409 T38404 npadvmod Optics,Probe
R9876 T38410 T38404 punct ", ",Probe
R9877 T38411 T38404 npadvmod Dunedin,Probe
R9878 T38412 T38404 punct ", ",Probe
R9879 T38413 T38404 npadvmod Florida,Probe
R9880 T38414 T38404 punct ", ",Probe
R9881 T38415 T38416 compound United,States
R9882 T38416 T38404 npadvmod States,Probe
R9883 T38417 T38404 punct ),Probe
R9884 T38418 T38394 punct .,measured
R9885 T38420 T38421 prep Following,determined
R9886 T38422 T38420 pobj measurement,Following
R9887 T38423 T38422 prep of,measurement
R9888 T38424 T38425 amod basal,respiration
R9889 T38425 T38423 pobj respiration,of
R9890 T38426 T38425 nmod state,respiration
R9891 T38427 T38426 nummod 2,state
R9892 T38428 T38421 punct ", ",determined
R9893 T38429 T38430 amod maximal,respiration
R9894 T38430 T38421 nsubjpass respiration,determined
R9895 T38431 T38430 punct (,respiration
R9896 T38432 T38433 npadvmod ADP,stimulated
R9897 T38433 T38430 amod stimulated,respiration
R9898 T38434 T38433 punct -,stimulated
R9899 T38435 T38430 punct ),respiration
R9900 T38436 T38430 nmod state,respiration
R9901 T38437 T38436 nummod 3,state
R9902 T38438 T38421 auxpass was,determined
R9903 T38439 T38421 prep by,determined
R9904 T38440 T38439 pcomp exposing,by
R9905 T38441 T38440 dobj fibers,exposing
R9906 T38442 T38440 prep to,exposing
R9907 T38443 T38444 nummod 1,mM
R9908 T38444 T38445 compound mM,ADP
R9909 T38445 T38442 pobj ADP,to
R9910 T38446 T38421 punct .,determined
R9911 T38448 T38449 det The,integrity
R9912 T38449 T38450 nsubjpass integrity,assessed
R9913 T38451 T38449 prep of,integrity
R9914 T38452 T38453 det the,membrane
R9915 T38453 T38451 pobj membrane,of
R9916 T38454 T38453 amod outer,membrane
R9917 T38455 T38453 amod mitochondrial,membrane
R9918 T38456 T38450 auxpass was,assessed
R9919 T38457 T38450 prep by,assessed
R9920 T38458 T38457 pcomp adding,by
R9921 T38459 T38460 nummod 8,μM
R9922 T38460 T38461 nmod μM,c
R9923 T38461 T38458 dobj c,adding
R9924 T38462 T38461 amod exogenous,c
R9925 T38463 T38461 compound cytochrome,c
R9926 T38464 T38458 prep to,adding
R9927 T38465 T38466 npadvmod ADP,stimulated
R9928 T38466 T38468 amod stimulated,fibers
R9929 T38467 T38466 punct -,stimulated
R9930 T38468 T38464 pobj fibers,to
R9931 T38469 T38450 punct .,assessed
R9932 T38471 T38472 nmod State,respiration
R9933 T38472 T38474 nsubjpass respiration,evaluated
R9934 T38473 T38471 nummod 4,State
R9935 T38475 T38476 punct (,uncoupled
R9936 T38476 T38472 parataxis uncoupled,respiration
R9937 T38477 T38476 punct ),uncoupled
R9938 T38478 T38474 auxpass was,evaluated
R9939 T38479 T38474 prep following,evaluated
R9940 T38480 T38479 pobj addition,following
R9941 T38481 T38480 prep of,addition
R9942 T38482 T38481 pobj oligomycin,of
R9943 T38483 T38484 punct (,μg
R9944 T38484 T38482 parataxis μg,oligomycin
R9945 T38485 T38484 nummod 1,μg
R9946 T38486 T38487 punct /,ml
R9947 T38487 T38484 prep ml,μg
R9948 T38488 T38484 punct ),μg
R9949 T38489 T38474 punct .,evaluated
R9950 T38491 T38492 det The,solubility
R9951 T38492 T38493 nsubjpass solubility,taken
R9952 T38494 T38492 prep of,solubility
R9953 T38495 T38494 pobj oxygen,of
R9954 T38496 T38492 prep in,solubility
R9955 T38497 T38498 det the,buffer
R9956 T38498 T38496 pobj buffer,in
R9957 T38499 T38498 compound respiration,buffer
R9958 T38500 T38492 prep at,solubility
R9959 T38501 T38502 nummod 25,°C
R9960 T38502 T38500 pobj °C,at
R9961 T38503 T38493 auxpass was,taken
R9962 T38504 T38493 prep as,taken
R9963 T38505 T38506 nummod 246.87,nmol
R9964 T38506 T38507 compound nmol,O2
R9965 T38507 T38504 pobj O2,as
R9966 T38508 T38507 punct ·,O2
R9967 T38509 T38507 appos ml,O2
R9968 T38510 T38511 punct −,1
R9969 T38511 T38509 nummod 1,ml
R9970 T38512 T38493 punct .,taken
R9971 T38514 T38515 compound Respiration,rates
R9972 T38515 T38516 nsubjpass rates,expressed
R9973 T38517 T38516 auxpass were,expressed
R9974 T38518 T38516 prep as,expressed
R9975 T38519 T38520 compound nmol,O2
R9976 T38520 T38518 pobj O2,as
R9977 T38521 T38520 punct ·,O2
R9978 T38522 T38520 appos min,O2
R9979 T38523 T38524 punct −,1
R9980 T38524 T38522 nummod 1,min
R9981 T38525 T38520 punct ·,O2
R9982 T38526 T38520 appos mgdw,O2
R9983 T38527 T38528 punct −,1
R9984 T38528 T38526 nummod 1,mgdw
R9985 T38529 T38516 punct .,expressed