PMC:3342329 / 33711-34891 JSONTXT 23 Projects

Annnotations TAB TSV DIC JSON TextAE

Id Subject Object Predicate Lexical cue
T16704 0-11 Entity denotes Lymphocytes
T16721 0-78 Binding denotes Lymphocytes were obtained from tumor and spleen tissues for phenotype analysis
T16717 91-108 Entity denotes tumor lymphocytes
T16709 180-184 Protein denotes mice
T16719 278-298 Entity denotes 100 µm cell strainer
T16715 348-373 Protein denotes ACK lysing buffer, LONZA)
T16700 352-358 Protein denotes lysing
T16710 384-399 Entity denotes red blood cells
T16699 401-424 Entity denotes One million splenocytes
T16706 460-489 Protein denotes 1% bovine serum albumin (BSA)
T16716 485-488 Protein denotes BSA
T16707 524-527 Protein denotes BSA
T16722 547-592 Binding denotes one million cells obtained from tumor tissues
T16696 547-592 Entity denotes one million cells obtained from tumor tissues
T16720 634-675 Binding denotes obtain suspension cell for 1hour at 37°C,
T16711 641-656 Entity denotes suspension cell
T16703 641-656 Entity denotes suspension cell
T16712 736-751 Entity denotes the lymphocytes
T16714 791-813 Protein denotes monoclonal antibodies,
T16698 824-831 Protein denotes CD4-APC
T16695 824-831 Entity denotes CD4-APC
T16701 833-841 Entity denotes CD8-FITC
T16697 833-841 Protein denotes CD8-FITC
T16713 972-1014 Protein denotes a FACSAria flow cytometry (BD Biosciences)
T16702 978-982 Protein denotes Aria
T16708 1082-1099 Protein denotes Scripps, La Jolla
T16718 1152-1179 Entity denotes used to gate on lymphocytes
T16705 1160-1164 Entity denotes gate