bionlp-st-ge-2016-test-ihmc  

PMC:3342329 / 26768-35896 JSONTXT 24 Projects

Annnotations TAB TSV DIC JSON TextAE

Id Subject Object Predicate Lexical cue
T13587 234-262 Protein denotes The CCR5 wild type (CCR5+/+)
T13582 267-271 Protein denotes CCR5
T13588 291-295 Protein denotes mice
T13585 370-378 Protein denotes The mice
T13576 521-542 Protein denotes The mice (n = 4/cage)
T13581 537-541 Protein denotes cage
T13580 745-761 Protein denotes The CCR5+/+ mice
T13579 763-767 Entity denotes n = 
T13589 775-787 Protein denotes CCR5−/− mice
T13574 789-793 Entity denotes n = 
T13575 903-905 Protein denotes F2
T13578 903-969 Protein denotes F2 hybrid mice from the C57BL/6J-AW-J and 129P3/J parental strains
T13584 971-975 Protein denotes CCR5
T13577 971-980 Protein denotes CCR5 mice
T13590 1036-1087 Regulation denotes of the CCR5 gene deleted from the parental strains,
T13583 1036-1087 Protein denotes of the CCR5 gene deleted from the parental strains,
T13586 1043-1047 Protein denotes CCR5
T14051 1175-1193 Entity denotes B16 melanoma cells
T14053 1224-1241 Entity denotes 5×105 tumor cells
T14055 1245-1261 Entity denotes 0.1 ml Phosphate
T14052 1271-1282 Entity denotes saline(PBS)
T14054 1339-1358 Protein denotes of the carrier mice
T14405 1817-1884 Negative_regulation denotes with protein extraction solution (PRO-PREPTM, Intron Biotechnology,
T14386 1822-1829 Protein denotes protein
T14393 1863-1883 Entity denotes Intron Biotechnology
T14403 1904-1915 Protein denotes lysed by 60
T14385 1934-1937 Entity denotes ice
T14377 2016-2035 Protein denotes of proteins (40 µg)
T14389 2054-2083 Entity denotes a SDS/12%-polyacrylamide gel,
T14383 2054-2083 Entity denotes a SDS/12%-polyacrylamide gel,
T14390 2056-2059 Protein denotes SDS
T14404 2108-2142 Entity denotes a polyvinylidene difluoride (PVDF)
T14373 2137-2141 Entity denotes PVDF
T14398 2143-2187 Entity denotes membrane (GE Water and Process technologies,
T14369 2197-2199 Protein denotes PA
T14384 2230-2286 Entity denotes 1 h at room temperature with 5% (w/v) non-fat dried milk
T14397 2259-2286 Entity denotes 5% (w/v) non-fat dried milk
T14399 2327-2337 Entity denotes 10 mM Tris
T14370 2351-2388 Entity denotes 150 mM NaCl solution containing 0.05%
T14387 2358-2362 Entity denotes NaCl
T14381 2431-2444 Entity denotes the membranes
T14388 2483-2530 Entity denotes primary antibodies: mouse monoclonal antibodies
T14372 2483-2530 Entity denotes primary antibodies: mouse monoclonal antibodies
T14395 2503-2530 Protein denotes mouse monoclonal antibodies
T14380 2548-2551 Protein denotes p65
T14396 2556-2559 Protein denotes p50
T14375 2578-2583 Entity denotes Santa
T14378 2628-2679 Entity denotes polyclonal antibodies directed against Bax and PARP
T14371 2667-2670 Protein denotes Bax
T14382 2675-2679 Protein denotes PARP
T14392 2698-2703 Entity denotes Santa
T14402 2737-2746 Protein denotes caspase-3
T14379 2748-2757 Protein denotes caspase-9
T14368 2759-2764 Protein denotes Bcl-2
T14401 2769-2776 Protein denotes c-IAP-1
T14400 2796-2800 Entity denotes Cell
T14394 2832-2834 Protein denotes MA
T14374 2894-2904 Protein denotes peroxidase
T14391 2992-3015 Protein denotes Immunoreactive proteins
T14376 3039-3042 Protein denotes ECL
T15409 3111-3119 Entity denotes formalin
T15391 3136-3144 Entity denotes paraffin
T15401 3248-3265 Entity denotes paraffin-embedded
T15411 3342-3391 Binding denotes then subjected to heat-mediated antigen retrieval
T15398 3374-3381 Entity denotes antigen
T15386 3404-3414 Protein denotes peroxidase
T15378 3454-3486 Entity denotes 3% hydrogen peroxide in methanol
T15381 3478-3486 Entity denotes methanol
T15385 3685-3718 Entity denotes cell nuclear antigen (PCNA, 1∶200
T15406 3690-3712 Protein denotes nuclear antigen (PCNA,
T15382 3707-3711 Protein denotes PCNA
T15408 3719-3727 Entity denotes dilution
T15405 3729-3750 Protein denotes monoclonal antibodies
T15400 3773-3776 Entity denotes 4 h
T15387 3926-3936 Entity denotes antibodies
T15379 3943-3944 Entity denotes h
T15407 4002-4041 Protein denotes of the avidin biotin peroxidase complex
T15404 4002-4041 Entity denotes of the avidin biotin peroxidase complex
T15396 4002-4041 Entity denotes of the avidin biotin peroxidase complex
T15383 4023-4033 Protein denotes peroxidase
T15394 4126-4136 Protein denotes peroxidase
T15403 4192-4201 Entity denotes peroxide,
T15395 4235-4246 Entity denotes hematoxylin
T15380 4260-4264 Entity denotes aqua
T15412 4356-4439 Positive_regulation denotes A negative control was included in all experiments by omitting the primary antibody
T15397 4419-4439 Entity denotes the primary antibody
T15389 4509-4526 Entity denotes paraffin-embedded
T15390 4554-4625 Entity denotes a mixture of the labeling solution (540 µl) and enzyme solution (60 µl)
T15384 4567-4597 Entity denotes the labeling solution (540 µl)
T15399 4602-4608 Protein denotes enzyme
T15410 4602-4625 Entity denotes enzyme solution (60 µl)
T15393 4630-4642 Entity denotes 1 h at 37°C,
T15402 4800-4821 Entity denotes 2-phenylindole (DAPI)
T15392 4816-4820 Entity denotes DAPI
T15388 4943-4946 Entity denotes DAS
T15682 5668-5671 Protein denotes Apo
T16156 5722-5753 Entity denotes Gel Electromobility Shift Assay
T16168 5754-5781 Entity denotes A gel electromobility shift
T16177 5934-5945 Entity denotes of solution
T16174 5949-5969 Entity denotes 10 mM HEPES [pH 7.9]
T16163 5978-5995 Entity denotes MgCl2, 10 mM KCl,
T16165 5985-5994 Entity denotes 10 mM KCl
T16167 5996-6055 Entity denotes 0.5 mM dithiothreitol, 0.2 mM phenylmethylsulfonylfluoride)
T16164 6019-6054 Entity denotes 0.2 mM phenylmethylsulfonylfluoride
T16179 6057-6065 Entity denotes vortexed
T16159 6091-6094 Entity denotes ice
T16181 6153-6172 Entity denotes The pelleted nuclei
T16169 6193-6263 Entity denotes solution C (solution A supplemented with 420 mM NaCl and 20% glycerol)
T16160 6234-6245 Entity denotes 420 mM NaCl
T16162 6250-6262 Entity denotes 20% glycerol
T16182 6279-6292 Entity denotes on ice for 20
T16180 6366-6408 Entity denotes the resulting nuclear extracts supernatant
T16155 6427-6451 Protein denotes a chilled Eppendorf tube
T16172 6453-6479 Entity denotes Consensus oligonucleotides
T16176 6485-6527 Protein denotes end-labeled using T4 polynucleotide kinase
T16173 6503-6505 Entity denotes T4
T16170 6533-6536 Entity denotes P32
T16157 6537-6552 Entity denotes -ATP for 10 min
T16175 6562-6585 Entity denotes The gel shift reactions
T16161 6658-6745 Entity denotes gel loading buffer was added to each reaction and loaded onto a 6% non-denaturating gel
T16154 6720-6745 Entity denotes a 6% non-denaturating gel
T16178 6747-6754 Entity denotes The gel
T16171 6834-6841 Entity denotes the gel
T16166 6843-6850 Entity denotes The gel
T16158 6865-6868 Entity denotes 1 h
T16704 6943-6954 Entity denotes Lymphocytes
T16721 6943-7021 Binding denotes Lymphocytes were obtained from tumor and spleen tissues for phenotype analysis
T16717 7034-7051 Entity denotes tumor lymphocytes
T16709 7123-7127 Protein denotes mice
T16719 7221-7241 Entity denotes 100 µm cell strainer
T16715 7291-7316 Protein denotes ACK lysing buffer, LONZA)
T16700 7295-7301 Protein denotes lysing
T16710 7327-7342 Entity denotes red blood cells
T16699 7344-7367 Entity denotes One million splenocytes
T16706 7403-7432 Protein denotes 1% bovine serum albumin (BSA)
T16716 7428-7431 Protein denotes BSA
T16707 7467-7470 Protein denotes BSA
T16722 7490-7535 Binding denotes one million cells obtained from tumor tissues
T16696 7490-7535 Entity denotes one million cells obtained from tumor tissues
T16720 7577-7618 Binding denotes obtain suspension cell for 1hour at 37°C,
T16711 7584-7599 Entity denotes suspension cell
T16703 7584-7599 Entity denotes suspension cell
T16712 7679-7694 Entity denotes the lymphocytes
T16714 7734-7756 Protein denotes monoclonal antibodies,
T16698 7767-7774 Protein denotes CD4-APC
T16695 7767-7774 Entity denotes CD4-APC
T16701 7776-7784 Entity denotes CD8-FITC
T16697 7776-7784 Protein denotes CD8-FITC
T16713 7915-7957 Protein denotes a FACSAria flow cytometry (BD Biosciences)
T16702 7921-7925 Protein denotes Aria
T16708 8025-8042 Protein denotes Scripps, La Jolla
T16718 8095-8122 Entity denotes used to gate on lymphocytes
T16705 8103-8107 Entity denotes gate
T16954 8125-8146 Entity denotes Protein Immuno-arrays
T16956 8217-8225 Protein denotes protease
T16961 8217-8265 Entity denotes protease inhibitor cocktail (Sigma-Aldrich, USA)
T16960 8246-8251 Protein denotes Sigma
T16959 8270-8307 Entity denotes Triton X-100 (final concentration 1%)
T16958 8450-8463 Protein denotes protein assay
T16957 8515-8564 Protein denotes proteins, collected from three samples per group,
T16955 8579-8599 Protein denotes Mouse cytokine array
T17078 8783-8786 Protein denotes Pad
T17076 8797-8799 Protein denotes La
T17077 8872-8876 Protein denotes mice
T17080 8881-8893 Protein denotes CCR5+/+ mice
T17079 8914-8915 Entity denotes t
T17075 9093-9103 Protein denotes Mantel-Cox
T17074 9113-9127 Protein denotes GraphPad Prism
R9291 T13583 T13590 themeOf "of the CCR5 gene deleted from the parental strains,","of the CCR5 gene deleted from the parental strains,"
R9971 T14386 T14405 themeOf protein,"with protein extraction solution (PRO-PREPTM, Intron Biotechnology,"