PMC:2817507 / 8457-9349 22 Projects
Annnotations
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T4181 | 0-3 | CD | denotes | 2.6 |
| T4182 | 3-4 | . | denotes | . |
| T4183 | 5-20 | NNP | denotes | Electrophoretic |
| T4184 | 21-29 | NNP | denotes | Mobility |
| T4185 | 30-35 | NNP | denotes | Shift |
| T4186 | 36-41 | NNP | denotes | Assay |
| T4187 | 42-43 | -LRB- | denotes | ( |
| T4188 | 43-47 | NNP | denotes | EMSA |
| T4189 | 47-48 | -RRB- | denotes | ) |
| T4190 | 49-56 | NNP | denotes | Nuclear |
| T4191 | 57-65 | NNS | denotes | proteins |
| T4192 | 66-70 | VBD | denotes | were |
| T4193 | 71-79 | VBN | denotes | isolated |
| T4194 | 80-84 | IN | denotes | from |
| T4195 | 85-90 | NN | denotes | brain |
| T4196 | 91-97 | NN | denotes | tissue |
| T4197 | 97-98 | . | denotes | . |
| T4198 | 99-106 | RB | denotes | Briefly |
| T4199 | 106-107 | , | denotes | , |
| T4200 | 108-113 | IN | denotes | after |
| T4201 | 114-121 | NN | denotes | removal |
| T4202 | 122-124 | IN | denotes | of |
| T4203 | 125-136 | JJ | denotes | supernatant |
| T4204 | 137-138 | -LRB- | denotes | ( |
| T4205 | 138-149 | JJ | denotes | cytoplasmic |
| T4206 | 150-157 | VB | denotes | extract |
| T4207 | 157-158 | -RRB- | denotes | ) |
| T4208 | 158-159 | , | denotes | , |
| T4209 | 160-163 | DT | denotes | the |
| T4210 | 164-171 | NNS | denotes | pellets |
| T4211 | 172-176 | VBD | denotes | were |
| T4212 | 177-188 | VBN | denotes | resuspended |
| T4213 | 189-191 | IN | denotes | in |
| T4214 | 192-199 | JJ | denotes | nuclear |
| T4215 | 200-210 | NN | denotes | extraction |
| T4216 | 211-218 | NN | denotes | reagent |
| T4217 | 218-219 | . | denotes | . |
| T4218 | 220-223 | DT | denotes | The |
| T4219 | 224-229 | NNS | denotes | tubes |
| T4220 | 230-240 | VBG | denotes | containing |
| T4221 | 241-248 | NNS | denotes | pellets |
| T4222 | 249-253 | VBD | denotes | were |
| T4223 | 254-257 | VBN | denotes | put |
| T4224 | 258-260 | IN | denotes | on |
| T4225 | 261-267 | NN | denotes | vortex |
| T4226 | 268-271 | IN | denotes | for |
| T4227 | 272-274 | CD | denotes | 15 |
| T4228 | 275-282 | NNS | denotes | seconds |
| T4229 | 283-288 | DT | denotes | every |
| T4230 | 289-291 | CD | denotes | 10 |
| T4231 | 292-299 | NNS | denotes | minutes |
| T4232 | 299-300 | , | denotes | , |
| T4233 | 301-304 | IN | denotes | for |
| T4234 | 305-306 | DT | denotes | a |
| T4235 | 307-312 | NN | denotes | total |
| T4236 | 313-315 | IN | denotes | of |
| T4237 | 316-318 | CD | denotes | 40 |
| T4238 | 319-326 | NNS | denotes | minutes |
| T4239 | 326-327 | . | denotes | . |
| T4240 | 328-331 | DT | denotes | The |
| T4241 | 332-337 | NNS | denotes | tubes |
| T4242 | 338-342 | VBD | denotes | were |
| T4243 | 343-354 | VBN | denotes | centrifuged |
| T4244 | 355-357 | IN | denotes | at |
| T4245 | 358-365 | JJ | denotes | maximum |
| T4246 | 366-371 | NN | denotes | speed |
| T4247 | 372-373 | -LRB- | denotes | ( |
| T4248 | 373-374 | FW | denotes | ~ |
| T4249 | 374-380 | CD | denotes | 16,000 |
| T4250 | 381-382 | CD | denotes | × |
| T4251 | 383-384 | NN | denotes | g |
| T4252 | 384-385 | -RRB- | denotes | ) |
| T4253 | 386-388 | IN | denotes | in |
| T4254 | 389-390 | DT | denotes | a |
| T4255 | 391-396 | NN | denotes | micro |
| T4256 | 397-407 | NN | denotes | centrifuge |
| T4257 | 408-411 | IN | denotes | for |
| T4258 | 412-414 | CD | denotes | 10 |
| T4259 | 415-422 | NNS | denotes | minutes |
| T4260 | 422-423 | . | denotes | . |
| T4261 | 424-427 | DT | denotes | The |
| T4262 | 428-440 | NNS | denotes | supernatants |
| T4263 | 441-442 | -LRB- | denotes | ( |
| T4264 | 442-449 | JJ | denotes | nuclear |
| T4265 | 450-457 | VB | denotes | extract |
| T4266 | 457-458 | -RRB- | denotes | ) |
| T4267 | 459-463 | VBD | denotes | were |
| T4268 | 464-475 | VBN | denotes | transferred |
| T4269 | 476-478 | TO | denotes | to |
| T4270 | 479-480 | DT | denotes | a |
| T4271 | 481-486 | JJ | denotes | clean |
| T4272 | 487-497 | JJ | denotes | prechilled |
| T4273 | 498-502 | NN | denotes | tube |
| T4274 | 503-506 | CC | denotes | and |
| T4275 | 507-513 | VBD | denotes | stored |
| T4276 | 514-516 | IN | denotes | at |
| T4277 | 517-518 | CD | denotes | − |
| T4278 | 518-520 | CD | denotes | 80 |
| T4279 | 520-521 | CD | denotes | ° |
| T4280 | 521-522 | NNP | denotes | C |
| T4281 | 523-528 | IN | denotes | until |
| T4282 | 529-532 | NN | denotes | use |
| T4283 | 532-533 | . | denotes | . |
| T4284 | 534-538 | NNP | denotes | EMSA |
| T4285 | 539-542 | VBD | denotes | was |
| T4286 | 543-552 | VBN | denotes | performed |
| T4287 | 553-555 | IN | denotes | as |
| T4288 | 556-565 | VBN | denotes | described |
| T4289 | 566-576 | RB | denotes | previously |
| T4290 | 577-578 | JJ | denotes | [ |
| T4291 | 578-579 | CD | denotes | 8 |
| T4292 | 579-580 | NN | denotes | ] |
| T4293 | 580-581 | . | denotes | . |
| T4294 | 582-586 | NNP | denotes | NFκB |
| T4295 | 587-594 | JJ | denotes | binding |
| T4296 | 595-603 | NN | denotes | activity |
| T4297 | 604-607 | VBD | denotes | was |
| T4298 | 608-616 | VBN | denotes | examined |
| T4299 | 617-619 | IN | denotes | in |
| T4300 | 620-621 | DT | denotes | a |
| T4301 | 622-624 | CD | denotes | 15 |
| T4302 | 625-627 | NN | denotes | μL |
| T4303 | 628-635 | JJ | denotes | binding |
| T4304 | 636-644 | NN | denotes | reaction |
| T4305 | 645-652 | NN | denotes | mixture |
| T4306 | 653-663 | VBG | denotes | containing |
| T4307 | 664-666 | CD | denotes | 15 |
| T4308 | 667-669 | NN | denotes | μg |
| T4309 | 670-672 | IN | denotes | of |
| T4310 | 673-680 | JJ | denotes | nuclear |
| T4311 | 681-689 | NNS | denotes | proteins |
| T4312 | 690-693 | CC | denotes | and |
| T4313 | 694-696 | CD | denotes | 35 |
| T4314 | 697-701 | NN | denotes | fmol |
| T4315 | 702-703 | NNP | denotes | [ |
| T4316 | 703-708 | NNP | denotes | γ-32P |
| T4317 | 708-709 | NNP | denotes | ] |
| T4318 | 710-717 | VBD | denotes | labeled |
| T4319 | 718-733 | JJ | denotes | double-stranded |
| T4320 | 734-738 | NNP | denotes | NFκB |
| T4321 | 739-748 | NN | denotes | consensus |
| T4322 | 749-764 | NN | denotes | oligonucleotide |
| T4323 | 764-765 | . | denotes | . |
| T4324 | 766-767 | DT | denotes | A |
| T4325 | 768-773 | JJ | denotes | super |
| T4326 | 774-779 | NN | denotes | shift |
| T4327 | 780-785 | NN | denotes | assay |
| T4328 | 786-791 | VBG | denotes | using |
| T4329 | 792-802 | NNS | denotes | antibodies |
| T4330 | 803-805 | TO | denotes | to |
| T4331 | 806-809 | NNP | denotes | P65 |
| T4332 | 810-813 | CC | denotes | and |
| T4333 | 814-817 | NNP | denotes | P50 |
| T4334 | 818-821 | VBD | denotes | was |
| T4335 | 822-831 | VBN | denotes | performed |
| T4336 | 832-834 | TO | denotes | to |
| T4337 | 835-842 | VB | denotes | confirm |
| T4338 | 843-847 | NNP | denotes | NFκB |
| T4339 | 848-855 | JJ | denotes | binding |
| T4340 | 856-867 | NN | denotes | specificity |
| T4341 | 868-870 | IN | denotes | as |
| T4342 | 871-881 | RB | denotes | previously |
| T4343 | 882-891 | VBN | denotes | described |
| T4344 | 891-892 | . | denotes | . |
| R3255 | T4181 | T4181 | ROOT | 2.6,2.6 |
| R3256 | T4182 | T4181 | punct | .,2.6 |
| R3257 | T4183 | T4186 | compound | Electrophoretic,Assay |
| R3258 | T4184 | T4185 | compound | Mobility,Shift |
| R3259 | T4185 | T4186 | compound | Shift,Assay |
| R3260 | T4186 | T4193 | nsubjpass | Assay,isolated |
| R3261 | T4187 | T4188 | punct | (,EMSA |
| R3262 | T4188 | T4186 | appos | EMSA,Assay |
| R3263 | T4189 | T4188 | punct | ),EMSA |
| R3264 | T4190 | T4191 | compound | Nuclear,proteins |
| R3265 | T4191 | T4192 | nsubj | proteins,were |
| R3266 | T4192 | T4193 | auxpass | were,isolated |
| R3267 | T4193 | T4193 | ROOT | isolated,isolated |
| R3268 | T4194 | T4193 | prep | from,isolated |
| R3269 | T4195 | T4196 | compound | brain,tissue |
| R3270 | T4196 | T4194 | pobj | tissue,from |
| R3271 | T4197 | T4193 | punct | .,isolated |
| R3272 | T4198 | T4206 | advmod | Briefly,extract |
| R3273 | T4199 | T4206 | punct | ",",extract |
| R3274 | T4200 | T4206 | prep | after,extract |
| R3275 | T4201 | T4200 | pobj | removal,after |
| R3276 | T4202 | T4201 | prep | of,removal |
| R3277 | T4203 | T4202 | pobj | supernatant,of |
| R3278 | T4204 | T4206 | punct | (,extract |
| R3279 | T4205 | T4206 | nsubj | cytoplasmic,extract |
| R3280 | T4206 | T4206 | ROOT | extract,extract |
| R3281 | T4207 | T4206 | punct | ),extract |
| R3282 | T4208 | T4212 | punct | ",",resuspended |
| R3283 | T4209 | T4210 | det | the,pellets |
| R3284 | T4210 | T4212 | nsubjpass | pellets,resuspended |
| R3285 | T4211 | T4212 | auxpass | were,resuspended |
| R3286 | T4212 | T4212 | ROOT | resuspended,resuspended |
| R3287 | T4213 | T4212 | prep | in,resuspended |
| R3288 | T4214 | T4215 | amod | nuclear,extraction |
| R3289 | T4215 | T4213 | pobj | extraction,in |
| R3290 | T4216 | T4212 | conj | reagent,resuspended |
| R3291 | T4217 | T4212 | punct | .,resuspended |
| R3292 | T4218 | T4219 | det | The,tubes |
| R3293 | T4219 | T4223 | nsubjpass | tubes,put |
| R3294 | T4220 | T4219 | acl | containing,tubes |
| R3295 | T4221 | T4220 | dobj | pellets,containing |
| R3296 | T4222 | T4223 | auxpass | were,put |
| R3297 | T4223 | T4223 | ROOT | put,put |
| R3298 | T4224 | T4223 | prep | on,put |
| R3299 | T4225 | T4224 | pobj | vortex,on |
| R3300 | T4226 | T4223 | prep | for,put |
| R3301 | T4227 | T4228 | nummod | 15,seconds |
| R3302 | T4228 | T4226 | pobj | seconds,for |
| R3303 | T4229 | T4231 | det | every,minutes |
| R3304 | T4230 | T4231 | nummod | 10,minutes |
| R3305 | T4231 | T4223 | npadvmod | minutes,put |
| R3306 | T4232 | T4231 | punct | ",",minutes |
| R3307 | T4233 | T4231 | prep | for,minutes |
| R3308 | T4234 | T4235 | det | a,total |
| R3309 | T4235 | T4233 | pobj | total,for |
| R3310 | T4236 | T4235 | prep | of,total |
| R3311 | T4237 | T4238 | nummod | 40,minutes |
| R3312 | T4238 | T4236 | pobj | minutes,of |
| R3313 | T4239 | T4223 | punct | .,put |
| R3314 | T4240 | T4241 | det | The,tubes |
| R3315 | T4241 | T4243 | nsubjpass | tubes,centrifuged |
| R3316 | T4242 | T4243 | auxpass | were,centrifuged |
| R3317 | T4243 | T4243 | ROOT | centrifuged,centrifuged |
| R3318 | T4244 | T4243 | prep | at,centrifuged |
| R3319 | T4245 | T4246 | amod | maximum,speed |
| R3320 | T4246 | T4244 | pobj | speed,at |
| R3321 | T4247 | T4251 | punct | (,g |
| R3322 | T4248 | T4251 | nmod | ~,g |
| R3323 | T4249 | T4251 | dep | "16,000",g |
| R3324 | T4250 | T4251 | nummod | ×,g |
| R3325 | T4251 | T4246 | appos | g,speed |
| R3326 | T4252 | T4251 | punct | ),g |
| R3327 | T4253 | T4243 | prep | in,centrifuged |
| R3328 | T4254 | T4256 | det | a,centrifuge |
| R3329 | T4255 | T4256 | amod | micro,centrifuge |
| R3330 | T4256 | T4253 | pobj | centrifuge,in |
| R3331 | T4257 | T4243 | prep | for,centrifuged |
| R3332 | T4258 | T4259 | nummod | 10,minutes |
| R3333 | T4259 | T4257 | pobj | minutes,for |
| R3334 | T4260 | T4243 | punct | .,centrifuged |
| R3335 | T4261 | T4262 | det | The,supernatants |
| R3336 | T4262 | T4268 | nsubjpass | supernatants,transferred |
| R3337 | T4263 | T4262 | punct | (,supernatants |
| R3338 | T4264 | T4265 | amod | nuclear,extract |
| R3339 | T4265 | T4262 | appos | extract,supernatants |
| R3340 | T4266 | T4262 | punct | ),supernatants |
| R3341 | T4267 | T4268 | auxpass | were,transferred |
| R3342 | T4268 | T4268 | ROOT | transferred,transferred |
| R3343 | T4269 | T4268 | prep | to,transferred |
| R3344 | T4270 | T4273 | det | a,tube |
| R3345 | T4271 | T4273 | amod | clean,tube |
| R3346 | T4272 | T4273 | amod | prechilled,tube |
| R3347 | T4273 | T4269 | pobj | tube,to |
| R3348 | T4274 | T4268 | cc | and,transferred |
| R3349 | T4275 | T4268 | conj | stored,transferred |
| R3350 | T4276 | T4275 | prep | at,stored |
| R3351 | T4277 | T4279 | nummod | −,° |
| R3352 | T4278 | T4279 | nummod | 80,° |
| R3353 | T4279 | T4276 | pobj | °,at |
| R3354 | T4280 | T4275 | npadvmod | C,stored |
| R3355 | T4281 | T4275 | prep | until,stored |
| R3356 | T4282 | T4281 | pobj | use,until |
| R3357 | T4283 | T4268 | punct | .,transferred |
| R3358 | T4284 | T4286 | nsubjpass | EMSA,performed |
| R3359 | T4285 | T4286 | auxpass | was,performed |
| R3360 | T4286 | T4286 | ROOT | performed,performed |
| R3361 | T4287 | T4288 | mark | as,described |
| R3362 | T4288 | T4286 | advcl | described,performed |
| R3363 | T4289 | T4290 | advmod | previously,[ |
| R3364 | T4290 | T4292 | amod | [,] |
| R3365 | T4291 | T4292 | nummod | 8,] |
| R3366 | T4292 | T4288 | dobj | ],described |
| R3367 | T4293 | T4286 | punct | .,performed |
| R3368 | T4294 | T4296 | amod | NFκB,activity |
| R3369 | T4295 | T4296 | amod | binding,activity |
| R3370 | T4296 | T4298 | nsubjpass | activity,examined |
| R3371 | T4297 | T4298 | auxpass | was,examined |
| R3372 | T4298 | T4298 | ROOT | examined,examined |
| R3373 | T4299 | T4298 | prep | in,examined |
| R3374 | T4300 | T4305 | det | a,mixture |
| R3375 | T4301 | T4302 | nummod | 15,μL |
| R3376 | T4302 | T4305 | compound | μL,mixture |
| R3377 | T4303 | T4304 | amod | binding,reaction |
| R3378 | T4304 | T4305 | compound | reaction,mixture |
| R3379 | T4305 | T4299 | pobj | mixture,in |
| R3380 | T4306 | T4305 | acl | containing,mixture |
| R3381 | T4307 | T4308 | nummod | 15,μg |
| R3382 | T4308 | T4317 | nmod | μg,] |
| R3383 | T4309 | T4308 | prep | of,μg |
| R3384 | T4310 | T4311 | amod | nuclear,proteins |
| R3385 | T4311 | T4309 | pobj | proteins,of |
| R3386 | T4312 | T4311 | cc | and,proteins |
| R3387 | T4313 | T4314 | nummod | 35,fmol |
| R3388 | T4314 | T4317 | compound | fmol,] |
| R3389 | T4315 | T4317 | compound | [,] |
| R3390 | T4316 | T4317 | compound | γ-32P,] |
| R3391 | T4317 | T4306 | dobj | ],containing |
| R3392 | T4318 | T4298 | conj | labeled,examined |
| R3393 | T4319 | T4322 | amod | double-stranded,oligonucleotide |
| R3394 | T4320 | T4321 | compound | NFκB,consensus |
| R3395 | T4321 | T4322 | compound | consensus,oligonucleotide |
| R3396 | T4322 | T4318 | oprd | oligonucleotide,labeled |
| R3397 | T4323 | T4298 | punct | .,examined |
| R3398 | T4324 | T4327 | det | A,assay |
| R3399 | T4325 | T4326 | amod | super,shift |
| R3400 | T4326 | T4327 | compound | shift,assay |
| R3401 | T4327 | T4335 | nsubjpass | assay,performed |
| R3402 | T4328 | T4327 | acl | using,assay |
| R3403 | T4329 | T4328 | dobj | antibodies,using |
| R3404 | T4330 | T4328 | prep | to,using |
| R3405 | T4331 | T4330 | pobj | P65,to |
| R3406 | T4332 | T4331 | cc | and,P65 |
| R3407 | T4333 | T4331 | conj | P50,P65 |
| R3408 | T4334 | T4335 | auxpass | was,performed |
| R3409 | T4335 | T4335 | ROOT | performed,performed |
| R3410 | T4336 | T4337 | aux | to,confirm |
| R3411 | T4337 | T4335 | xcomp | confirm,performed |
| R3412 | T4338 | T4340 | nmod | NFκB,specificity |
| R3413 | T4339 | T4340 | amod | binding,specificity |
| R3414 | T4340 | T4337 | dobj | specificity,confirm |
| R3415 | T4341 | T4343 | mark | as,described |
| R3416 | T4342 | T4343 | advmod | previously,described |
| R3417 | T4343 | T4340 | advcl | described,specificity |
| R3418 | T4344 | T4335 | punct | .,performed |