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PMC:2664230 / 10802-12526 JSONTXT 7 Projects

Annnotations TAB TSV DIC JSON TextAE

Id Subject Object Predicate Lexical cue
T4907 0-5 NN denotes NF-κB
T4908 6-9 CC denotes and
T4909 10-14 NNP denotes CREB
T4910 15-30 NNP denotes Electrophoretic
T4911 31-39 NNP denotes Mobility
T4912 40-45 NNP denotes Shift
T4913 46-51 NNP denotes Assay
T4914 52-53 -LRB- denotes (
T4915 53-57 NNP denotes EMSA
T4916 57-58 -RRB- denotes )
T4917 59-62 DT denotes The
T4918 63-78 JJ denotes non-radioactive
T4919 79-89 NNP denotes LightShift
T4920 90-106 NNP denotes Chemiluminescent
T4921 107-111 NNP denotes EMSA
T4922 112-115 NN denotes Kit
T4923 116-119 VBD denotes was
T4924 120-124 VBN denotes used
T4925 125-127 TO denotes to
T4926 128-134 VB denotes detect
T4927 135-146 JJ denotes DNA-protein
T4928 147-159 NNS denotes interactions
T4929 159-160 . denotes .
T4930 161-164 DT denotes The
T4931 165-170 NN denotes NF-κB
T4932 171-172 CD denotes 3
T4933 172-173 NN denotes ′
T4934 174-180 NN denotes biotin
T4935 181-192 VBD denotes end-labeled
T4936 193-196 NNP denotes DNA
T4937 197-212 NN denotes oligonucleotide
T4938 213-217 VBD denotes used
T4939 218-220 IN denotes as
T4940 221-222 DT denotes a
T4941 223-228 NN denotes probe
T4942 229-232 IN denotes for
T4943 233-236 DT denotes the
T4944 237-241 NNP denotes EMSA
T4945 242-245 VBD denotes was
T4946 246-247 DT denotes a
T4947 248-253 JJ denotes 42-bp
T4948 254-260 JJ denotes double
T4949 261-269 VBN denotes stranded
T4950 270-279 VBP denotes construct
T4951 280-281 -LRB- denotes (
T4952 281-282 CD denotes 5
T4953 282-283 SYM denotes ′
T4954 283-284 : denotes -
T4955 284-327 NN denotes TTGTTACAA-GGGGACTTTCCGCTGGGGACTTTCCAGGGAGGC
T4956 330-331 CD denotes 3
T4957 331-332 NN denotes ′
T4958 332-333 -RRB- denotes )
T4959 334-344 VBG denotes containing
T4960 345-348 CD denotes two
T4961 349-357 JJ denotes tandemly
T4962 358-366 VBN denotes repeated
T4963 367-372 NN denotes NF-κB
T4964 373-380 JJ denotes binding
T4965 381-386 NNS denotes sites
T4966 387-388 -LRB- denotes (
T4967 388-398 VBN denotes underlined
T4968 398-399 -RRB- denotes )
T4969 399-400 . denotes .
T4970 401-412 NN denotes Specificity
T4971 413-416 VBD denotes was
T4972 417-427 VBN denotes determined
T4973 428-430 IN denotes by
T4974 431-432 DT denotes a
T4975 433-444 NN denotes competition
T4976 445-450 NN denotes assay
T4977 451-455 IN denotes with
T4978 456-459 DT denotes the
T4979 460-468 NN denotes addition
T4980 469-471 IN denotes of
T4981 472-475 CD denotes 200
T4982 476-481 JJ denotes molar
T4983 482-488 NN denotes excess
T4984 489-491 IN denotes of
T4985 492-501 JJ denotes unlabeled
T4986 502-508 JJ denotes double
T4987 509-517 VBN denotes stranded
T4988 518-523 NN denotes NF-κB
T4989 524-539 NN denotes oligonucleotide
T4990 539-540 . denotes .
T4991 541-544 DT denotes The
T4992 545-549 NN denotes CREB
T4993 550-551 CD denotes 3
T4994 551-552 NN denotes ′
T4995 553-559 NN denotes biotin
T4996 560-571 VBD denotes end-labeled
T4997 572-575 NNP denotes DNA
T4998 576-591 NN denotes oligonucleotide
T4999 592-596 VBD denotes used
T5000 597-599 IN denotes as
T5001 600-601 DT denotes a
T5002 602-607 NN denotes probe
T5003 608-611 IN denotes for
T5004 612-615 DT denotes the
T5005 616-620 NNP denotes EMSA
T5006 621-624 VBD denotes was
T5007 625-626 DT denotes a
T5008 627-632 JJ denotes 23-bp
T5009 633-639 JJ denotes double
T5010 640-648 VBN denotes stranded
T5011 649-658 VBP denotes construct
T5012 659-660 -LRB- denotes (
T5013 660-661 CD denotes 5
T5014 661-662 SYM denotes ′
T5015 662-663 : denotes -
T5016 663-666 NNP denotes TTT
T5017 667-670 NNP denotes TCG
T5018 671-674 NNP denotes AGC
T5019 675-690 NNP denotes TCTGACGTCAGA-GC
T5020 693-694 CD denotes 3
T5021 694-695 NN denotes ′
T5022 695-696 -RRB- denotes )
T5023 697-707 VBG denotes containing
T5024 708-711 DT denotes the
T5025 712-715 NNP denotes CRE
T5026 716-725 NN denotes consensus
T5027 726-734 NN denotes sequence
T5028 735-736 -LRB- denotes (
T5029 736-746 VBN denotes underlined
T5030 746-747 -RRB- denotes )
T5031 747-748 . denotes .
T5032 749-760 NN denotes Specificity
T5033 761-764 VBD denotes was
T5034 765-775 VBN denotes determined
T5035 776-778 IN denotes by
T5036 779-780 DT denotes a
T5037 781-792 NN denotes competition
T5038 793-798 NN denotes assay
T5039 799-803 IN denotes with
T5040 804-807 DT denotes the
T5041 808-816 NN denotes addition
T5042 817-819 IN denotes of
T5043 820-823 CD denotes 200
T5044 824-829 JJ denotes molar
T5045 830-836 NN denotes excess
T5046 837-839 IN denotes of
T5047 840-849 JJ denotes unlabeled
T5048 850-856 JJ denotes double
T5049 857-865 VBN denotes stranded
T5050 866-870 NNP denotes CREB
T5051 871-886 NN denotes oligonucleotide
T5052 886-887 . denotes .
T5053 888-895 NNP denotes Nuclear
T5054 896-904 NNS denotes extracts
T5055 905-906 -LRB- denotes (
T5056 906-908 CD denotes 10
T5057 909-911 NN denotes μg
T5058 911-912 -RRB- denotes )
T5059 913-917 VBD denotes were
T5060 918-927 VBN denotes incubated
T5061 928-932 IN denotes with
T5062 933-934 CD denotes 5
T5063 935-937 NNP denotes nM
T5064 938-943 NNP denotes NF-κB
T5065 944-946 CC denotes or
T5066 947-951 NNP denotes CREB
T5067 952-957 NN denotes probe
T5068 958-959 -LRB- denotes (
T5069 959-964 NN denotes NF-kB
T5070 964-965 : denotes :
T5071 966-968 CD denotes 1x
T5072 969-976 JJ denotes binding
T5073 977-983 NN denotes buffer
T5074 983-984 , denotes ,
T5075 985-987 CD denotes 50
T5076 988-990 NNP denotes mM
T5077 991-994 NNP denotes KCl
T5078 994-995 , denotes ,
T5079 996-997 CD denotes 1
T5080 998-1000 NNP denotes mM
T5081 1001-1005 NNP denotes EDTA
T5082 1005-1006 , denotes ,
T5083 1007-1008 CD denotes 1
T5084 1009-1011 NNP denotes mM
T5085 1012-1015 NNP denotes DTT
T5086 1015-1016 , denotes ,
T5087 1017-1020 CD denotes 0.1
T5088 1020-1021 NN denotes %
T5089 1022-1026 CD denotes NP40
T5090 1026-1027 , denotes ,
T5091 1028-1030 CD denotes 10
T5092 1030-1031 NN denotes %
T5093 1032-1040 NN denotes glycerol
T5094 1040-1041 , denotes ,
T5095 1042-1045 CC denotes and
T5096 1046-1048 CD denotes 50
T5097 1049-1051 NN denotes ng
T5098 1051-1052 NN denotes /
T5099 1052-1054 NN denotes μl
T5100 1055-1059 NN denotes poly
T5101 1060-1065 NN denotes dI-dC
T5102 1065-1066 , denotes ,
T5103 1067-1071 NNP denotes CREB
T5104 1071-1072 : denotes :
T5105 1073-1075 JJ denotes 1x
T5106 1076-1083 JJ denotes binding
T5107 1084-1090 NN denotes buffer
T5108 1090-1091 , denotes ,
T5109 1092-1094 CD denotes 20
T5110 1095-1097 NNP denotes mM
T5111 1098-1102 NNP denotes Tris
T5112 1102-1103 , denotes ,
T5113 1104-1106 NNP denotes pH
T5114 1107-1110 CD denotes 7.5
T5115 1110-1111 , denotes ,
T5116 1112-1114 CD denotes 50
T5117 1115-1117 NNP denotes mM
T5118 1118-1121 NNP denotes KCl
T5119 1121-1122 , denotes ,
T5120 1123-1124 CD denotes 1
T5121 1125-1127 NNP denotes mM
T5122 1128-1132 NNP denotes EDTA
T5123 1132-1133 , denotes ,
T5124 1134-1135 CD denotes 1
T5125 1136-1138 NNP denotes mM
T5126 1139-1142 NNP denotes DTT
T5127 1142-1143 , denotes ,
T5128 1144-1148 CD denotes 0.10
T5129 1148-1149 NN denotes %
T5130 1150-1154 CD denotes NP40
T5131 1154-1155 , denotes ,
T5132 1156-1157 CD denotes 6
T5133 1157-1158 NN denotes %
T5134 1159-1167 NN denotes glycerol
T5135 1167-1168 , denotes ,
T5136 1169-1172 CD denotes 0.1
T5137 1173-1178 NNP denotes mg/mL
T5138 1179-1182 NNP denotes BSA
T5139 1183-1186 CC denotes and
T5140 1187-1189 CD denotes 50
T5141 1190-1192 NN denotes ng
T5142 1192-1193 NN denotes /
T5143 1193-1195 NN denotes μl
T5144 1196-1200 NN denotes poly
T5145 1201-1206 NN denotes dI-dC
T5146 1206-1207 -RRB- denotes )
T5147 1208-1211 CC denotes and
T5148 1212-1216 VBD denotes were
T5149 1217-1221 RB denotes then
T5150 1222-1237 VBN denotes electrophoresed
T5151 1238-1245 IN denotes through
T5152 1246-1247 DT denotes a
T5153 1248-1249 CD denotes 6
T5154 1249-1250 NN denotes %
T5155 1251-1254 NN denotes DNA
T5156 1255-1266 NN denotes retardation
T5157 1267-1270 NN denotes gel
T5158 1271-1273 IN denotes at
T5159 1274-1278 CD denotes 100V
T5160 1279-1282 IN denotes for
T5161 1283-1285 CD denotes 90
T5162 1286-1293 NNS denotes minutes
T5163 1293-1294 . denotes .
T5164 1295-1298 DT denotes The
T5165 1299-1303 NNS denotes gels
T5166 1304-1308 VBD denotes were
T5167 1309-1328 RB denotes electrophoretically
T5168 1329-1340 VBN denotes transferred
T5169 1341-1343 IN denotes at
T5170 1344-1349 NNP denotes 380mA
T5171 1350-1353 IN denotes for
T5172 1354-1355 CD denotes 1
T5173 1356-1360 NN denotes hour
T5174 1361-1363 IN denotes on
T5175 1364-1367 NN denotes ice
T5176 1368-1370 TO denotes to
T5177 1371-1372 DT denotes a
T5178 1373-1383 RB denotes positively
T5179 1384-1391 VBN denotes charged
T5180 1392-1397 NN denotes nylon
T5181 1398-1406 NN denotes membrane
T5182 1407-1410 CC denotes and
T5183 1411-1422 RB denotes immediately
T5184 1423-1435 JJ denotes cross-linked
T5185 1436-1439 IN denotes for
T5186 1440-1442 CD denotes 15
T5187 1443-1450 NNS denotes minutes
T5188 1451-1455 IN denotes with
T5189 1456-1457 DT denotes a
T5190 1458-1460 NNP denotes UV
T5191 1461-1477 NN denotes transilluminator
T5192 1478-1486 VBN denotes equipped
T5193 1487-1491 IN denotes with
T5194 1492-1493 DT denotes a
T5195 1494-1497 CD denotes 312
T5196 1498-1500 NN denotes nm
T5197 1501-1505 NN denotes bulb
T5198 1505-1506 . denotes .
T5199 1507-1531 JJ denotes Streptavidin-horseradish
T5200 1532-1542 NN denotes peroxidase
T5201 1543-1552 NN denotes conjugate
T5202 1553-1556 CC denotes and
T5203 1557-1560 DT denotes the
T5204 1561-1571 NNP denotes LightShift
T5205 1572-1588 NNP denotes Chemiluminescent
T5206 1589-1598 NNP denotes Substrate
T5207 1599-1603 VBD denotes were
T5208 1604-1608 VBN denotes used
T5209 1609-1611 TO denotes to
T5210 1612-1618 VB denotes detect
T5211 1619-1622 DT denotes the
T5212 1623-1629 NN denotes biotin
T5213 1630-1641 VBD denotes end-labeled
T5214 1642-1645 NNP denotes DNA
T5215 1645-1646 . denotes .
T5216 1647-1650 DT denotes The
T5217 1651-1656 NN denotes nylon
T5218 1657-1666 NNS denotes membranes
T5219 1667-1671 VBD denotes were
T5220 1672-1679 VBN denotes exposed
T5221 1680-1682 TO denotes to
T5222 1683-1688 VB denotes x-ray
T5223 1689-1693 NN denotes film
T5224 1694-1697 IN denotes for
T5225 1698-1699 CD denotes 1
T5226 1700-1701 CD denotes 3
T5227 1702-1709 NNS denotes minutes
T5228 1710-1713 IN denotes for
T5229 1714-1723 NN denotes detection
T5230 1723-1724 . denotes .
R4034 T4907 T4907 ROOT NF-κB,NF-κB
R4035 T4908 T4907 cc and,NF-κB
R4036 T4909 T4913 compound CREB,Assay
R4037 T4910 T4913 compound Electrophoretic,Assay
R4038 T4911 T4912 compound Mobility,Shift
R4039 T4912 T4913 compound Shift,Assay
R4040 T4913 T4907 conj Assay,NF-κB
R4041 T4914 T4915 punct (,EMSA
R4042 T4915 T4913 appos EMSA,Assay
R4043 T4916 T4913 punct ),Assay
R4044 T4917 T4922 det The,Kit
R4045 T4918 T4922 amod non-radioactive,Kit
R4046 T4919 T4921 compound LightShift,EMSA
R4047 T4920 T4921 compound Chemiluminescent,EMSA
R4048 T4921 T4922 compound EMSA,Kit
R4049 T4922 T4924 nsubjpass Kit,used
R4050 T4923 T4924 auxpass was,used
R4051 T4924 T4924 ROOT used,used
R4052 T4925 T4926 aux to,detect
R4053 T4926 T4924 xcomp detect,used
R4054 T4927 T4928 amod DNA-protein,interactions
R4055 T4928 T4926 dobj interactions,detect
R4056 T4929 T4924 punct .,used
R4057 T4930 T4934 det The,biotin
R4058 T4931 T4934 nmod NF-κB,biotin
R4059 T4932 T4933 nummod 3,′
R4060 T4933 T4934 compound ′,biotin
R4061 T4934 T4935 nsubj biotin,end-labeled
R4062 T4935 T4935 ROOT end-labeled,end-labeled
R4063 T4936 T4937 compound DNA,oligonucleotide
R4064 T4937 T4945 nsubj oligonucleotide,was
R4065 T4938 T4937 acl used,oligonucleotide
R4066 T4939 T4938 prep as,used
R4067 T4940 T4941 det a,probe
R4068 T4941 T4939 pobj probe,as
R4069 T4942 T4941 prep for,probe
R4070 T4943 T4944 det the,EMSA
R4071 T4944 T4942 pobj EMSA,for
R4072 T4945 T4935 ccomp was,end-labeled
R4073 T4946 T4950 det a,construct
R4074 T4947 T4949 amod 42-bp,stranded
R4075 T4948 T4949 amod double,stranded
R4076 T4949 T4950 amod stranded,construct
R4077 T4950 T4945 attr construct,was
R4078 T4951 T4955 punct (,TTGTTACAA-GGGGACTTTCCGCTGGGGACTTTCCAGGGAGGC
R4079 T4952 T4955 nummod 5,TTGTTACAA-GGGGACTTTCCGCTGGGGACTTTCCAGGGAGGC
R4080 T4953 T4955 punct ′,TTGTTACAA-GGGGACTTTCCGCTGGGGACTTTCCAGGGAGGC
R4081 T4954 T4955 punct -,TTGTTACAA-GGGGACTTTCCGCTGGGGACTTTCCAGGGAGGC
R4082 T4955 T4959 meta TTGTTACAA-GGGGACTTTCCGCTGGGGACTTTCCAGGGAGGC,containing
R4083 T4956 T4957 nummod 3,′
R4084 T4957 T4955 appos ′,TTGTTACAA-GGGGACTTTCCGCTGGGGACTTTCCAGGGAGGC
R4085 T4958 T4955 punct ),TTGTTACAA-GGGGACTTTCCGCTGGGGACTTTCCAGGGAGGC
R4086 T4959 T4950 acl containing,construct
R4087 T4960 T4965 nummod two,sites
R4088 T4961 T4965 amod tandemly,sites
R4089 T4962 T4963 amod repeated,NF-κB
R4090 T4963 T4965 amod NF-κB,sites
R4091 T4964 T4965 amod binding,sites
R4092 T4965 T4959 dobj sites,containing
R4093 T4966 T4965 punct (,sites
R4094 T4967 T4965 acl underlined,sites
R4095 T4968 T4965 punct ),sites
R4096 T4969 T4945 punct .,was
R4097 T4970 T4972 nsubjpass Specificity,determined
R4098 T4971 T4972 auxpass was,determined
R4099 T4972 T4972 ROOT determined,determined
R4100 T4973 T4972 agent by,determined
R4101 T4974 T4976 det a,assay
R4102 T4975 T4976 compound competition,assay
R4103 T4976 T4973 pobj assay,by
R4104 T4977 T4976 prep with,assay
R4105 T4978 T4979 det the,addition
R4106 T4979 T4977 pobj addition,with
R4107 T4980 T4979 prep of,addition
R4108 T4981 T4983 nummod 200,excess
R4109 T4982 T4983 amod molar,excess
R4110 T4983 T4980 pobj excess,of
R4111 T4984 T4983 prep of,excess
R4112 T4985 T4989 amod unlabeled,oligonucleotide
R4113 T4986 T4989 amod double,oligonucleotide
R4114 T4987 T4989 amod stranded,oligonucleotide
R4115 T4988 T4989 compound NF-κB,oligonucleotide
R4116 T4989 T4984 pobj oligonucleotide,of
R4117 T4990 T4972 punct .,determined
R4118 T4991 T4995 det The,biotin
R4119 T4992 T4995 nmod CREB,biotin
R4120 T4993 T4994 nummod 3,′
R4121 T4994 T4995 compound ′,biotin
R4122 T4995 T4996 nsubj biotin,end-labeled
R4123 T4996 T4996 ROOT end-labeled,end-labeled
R4124 T4997 T4998 compound DNA,oligonucleotide
R4125 T4998 T4999 nsubj oligonucleotide,used
R4126 T4999 T4996 conj used,end-labeled
R4127 T5000 T4999 prep as,used
R4128 T5001 T5002 det a,probe
R4129 T5002 T5000 pobj probe,as
R4130 T5003 T5002 prep for,probe
R4131 T5004 T5005 det the,EMSA
R4132 T5005 T5003 pobj EMSA,for
R4133 T5006 T4996 conj was,end-labeled
R4134 T5007 T5011 det a,construct
R4135 T5008 T5010 amod 23-bp,stranded
R4136 T5009 T5010 amod double,stranded
R4137 T5010 T5011 amod stranded,construct
R4138 T5011 T5006 attr construct,was
R4139 T5012 T5019 punct (,TCTGACGTCAGA-GC
R4140 T5013 T5019 nummod 5,TCTGACGTCAGA-GC
R4141 T5014 T5019 punct ′,TCTGACGTCAGA-GC
R4142 T5015 T5019 punct -,TCTGACGTCAGA-GC
R4143 T5016 T5019 compound TTT,TCTGACGTCAGA-GC
R4144 T5017 T5019 compound TCG,TCTGACGTCAGA-GC
R4145 T5018 T5019 compound AGC,TCTGACGTCAGA-GC
R4146 T5019 T5011 appos TCTGACGTCAGA-GC,construct
R4147 T5020 T5021 nummod 3,′
R4148 T5021 T5019 appos ′,TCTGACGTCAGA-GC
R4149 T5022 T5021 punct ),′
R4150 T5023 T5019 acl containing,TCTGACGTCAGA-GC
R4151 T5024 T5027 det the,sequence
R4152 T5025 T5027 compound CRE,sequence
R4153 T5026 T5027 compound consensus,sequence
R4154 T5027 T5023 dobj sequence,containing
R4155 T5028 T5029 punct (,underlined
R4156 T5029 T5019 parataxis underlined,TCTGACGTCAGA-GC
R4157 T5030 T5029 punct ),underlined
R4158 T5031 T4996 punct .,end-labeled
R4159 T5032 T5034 nsubjpass Specificity,determined
R4160 T5033 T5034 auxpass was,determined
R4161 T5034 T5034 ROOT determined,determined
R4162 T5035 T5034 agent by,determined
R4163 T5036 T5038 det a,assay
R4164 T5037 T5038 compound competition,assay
R4165 T5038 T5035 pobj assay,by
R4166 T5039 T5038 prep with,assay
R4167 T5040 T5041 det the,addition
R4168 T5041 T5039 pobj addition,with
R4169 T5042 T5041 prep of,addition
R4170 T5043 T5045 nummod 200,excess
R4171 T5044 T5045 amod molar,excess
R4172 T5045 T5042 pobj excess,of
R4173 T5046 T5045 prep of,excess
R4174 T5047 T5048 amod unlabeled,double
R4175 T5048 T5051 amod double,oligonucleotide
R4176 T5049 T5051 amod stranded,oligonucleotide
R4177 T5050 T5051 compound CREB,oligonucleotide
R4178 T5051 T5046 pobj oligonucleotide,of
R4179 T5052 T5034 punct .,determined
R4180 T5053 T5054 compound Nuclear,extracts
R4181 T5054 T5060 nsubjpass extracts,incubated
R4182 T5055 T5054 punct (,extracts
R4183 T5056 T5057 nummod 10,μg
R4184 T5057 T5054 appos μg,extracts
R4185 T5058 T5054 punct ),extracts
R4186 T5059 T5060 auxpass were,incubated
R4187 T5060 T5060 ROOT incubated,incubated
R4188 T5061 T5060 prep with,incubated
R4189 T5062 T5064 nummod 5,NF-κB
R4190 T5063 T5064 compound nM,NF-κB
R4191 T5064 T5061 pobj NF-κB,with
R4192 T5065 T5064 cc or,NF-κB
R4193 T5066 T5067 compound CREB,probe
R4194 T5067 T5064 conj probe,NF-κB
R4195 T5068 T5150 punct (,electrophoresed
R4196 T5069 T5150 nsubjpass NF-kB,electrophoresed
R4197 T5070 T5069 punct :,NF-kB
R4198 T5071 T5073 nummod 1x,buffer
R4199 T5072 T5073 amod binding,buffer
R4200 T5073 T5069 appos buffer,NF-kB
R4201 T5074 T5073 punct ",",buffer
R4202 T5075 T5077 nummod 50,KCl
R4203 T5076 T5077 compound mM,KCl
R4204 T5077 T5073 appos KCl,buffer
R4205 T5078 T5077 punct ",",KCl
R4206 T5079 T5081 nummod 1,EDTA
R4207 T5080 T5081 compound mM,EDTA
R4208 T5081 T5077 appos EDTA,KCl
R4209 T5082 T5081 punct ",",EDTA
R4210 T5083 T5085 nummod 1,DTT
R4211 T5084 T5085 compound mM,DTT
R4212 T5085 T5081 appos DTT,EDTA
R4213 T5086 T5085 punct ",",DTT
R4214 T5087 T5088 nummod 0.1,%
R4215 T5088 T5085 appos %,DTT
R4216 T5089 T5093 nummod NP40,glycerol
R4217 T5090 T5093 punct ",",glycerol
R4218 T5091 T5092 nummod 10,%
R4219 T5092 T5093 compound %,glycerol
R4220 T5093 T5085 conj glycerol,DTT
R4221 T5094 T5093 punct ",",glycerol
R4222 T5095 T5093 cc and,glycerol
R4223 T5096 T5097 nummod 50,ng
R4224 T5097 T5101 amod ng,dI-dC
R4225 T5098 T5101 nmod /,dI-dC
R4226 T5099 T5101 compound μl,dI-dC
R4227 T5100 T5101 compound poly,dI-dC
R4228 T5101 T5093 conj dI-dC,glycerol
R4229 T5102 T5101 punct ",",dI-dC
R4230 T5103 T5111 nmod CREB,Tris
R4231 T5104 T5107 punct :,buffer
R4232 T5105 T5107 amod 1x,buffer
R4233 T5106 T5107 amod binding,buffer
R4234 T5107 T5111 nmod buffer,Tris
R4235 T5108 T5111 punct ",",Tris
R4236 T5109 T5111 nummod 20,Tris
R4237 T5110 T5111 compound mM,Tris
R4238 T5111 T5101 appos Tris,dI-dC
R4239 T5112 T5111 punct ",",Tris
R4240 T5113 T5111 conj pH,Tris
R4241 T5114 T5113 nummod 7.5,pH
R4242 T5115 T5113 punct ",",pH
R4243 T5116 T5118 nummod 50,KCl
R4244 T5117 T5118 compound mM,KCl
R4245 T5118 T5113 conj KCl,pH
R4246 T5119 T5118 punct ",",KCl
R4247 T5120 T5122 nummod 1,EDTA
R4248 T5121 T5122 compound mM,EDTA
R4249 T5122 T5118 conj EDTA,KCl
R4250 T5123 T5122 punct ",",EDTA
R4251 T5124 T5126 nummod 1,DTT
R4252 T5125 T5126 compound mM,DTT
R4253 T5126 T5122 conj DTT,EDTA
R4254 T5127 T5126 punct ",",DTT
R4255 T5128 T5129 nummod 0.10,%
R4256 T5129 T5126 appos %,DTT
R4257 T5130 T5134 nummod NP40,glycerol
R4258 T5131 T5130 punct ",",NP40
R4259 T5132 T5133 nummod 6,%
R4260 T5133 T5134 compound %,glycerol
R4261 T5134 T5126 conj glycerol,DTT
R4262 T5135 T5134 punct ",",glycerol
R4263 T5136 T5138 nummod 0.1,BSA
R4264 T5137 T5138 compound mg/mL,BSA
R4265 T5138 T5134 conj BSA,glycerol
R4266 T5139 T5138 cc and,BSA
R4267 T5140 T5141 nummod 50,ng
R4268 T5141 T5145 nmod ng,dI-dC
R4269 T5142 T5145 nmod /,dI-dC
R4270 T5143 T5145 nmod μl,dI-dC
R4271 T5144 T5145 compound poly,dI-dC
R4272 T5145 T5138 conj dI-dC,BSA
R4273 T5146 T5145 punct ),dI-dC
R4274 T5147 T5101 cc and,dI-dC
R4275 T5148 T5150 auxpass were,electrophoresed
R4276 T5149 T5150 advmod then,electrophoresed
R4277 T5150 T5150 ROOT electrophoresed,electrophoresed
R4278 T5151 T5150 prep through,electrophoresed
R4279 T5152 T5157 det a,gel
R4280 T5153 T5154 nummod 6,%
R4281 T5154 T5157 compound %,gel
R4282 T5155 T5156 compound DNA,retardation
R4283 T5156 T5157 compound retardation,gel
R4284 T5157 T5151 pobj gel,through
R4285 T5158 T5157 prep at,gel
R4286 T5159 T5158 pobj 100V,at
R4287 T5160 T5150 prep for,electrophoresed
R4288 T5161 T5162 nummod 90,minutes
R4289 T5162 T5160 pobj minutes,for
R4290 T5163 T5150 punct .,electrophoresed
R4291 T5164 T5165 det The,gels
R4292 T5165 T5168 nsubjpass gels,transferred
R4293 T5166 T5168 auxpass were,transferred
R4294 T5167 T5168 advmod electrophoretically,transferred
R4295 T5168 T5168 ROOT transferred,transferred
R4296 T5169 T5168 prep at,transferred
R4297 T5170 T5169 pobj 380mA,at
R4298 T5171 T5168 prep for,transferred
R4299 T5172 T5173 nummod 1,hour
R4300 T5173 T5171 pobj hour,for
R4301 T5174 T5173 prep on,hour
R4302 T5175 T5174 pobj ice,on
R4303 T5176 T5168 prep to,transferred
R4304 T5177 T5181 det a,membrane
R4305 T5178 T5179 advmod positively,charged
R4306 T5179 T5181 amod charged,membrane
R4307 T5180 T5181 compound nylon,membrane
R4308 T5181 T5176 pobj membrane,to
R4309 T5182 T5181 cc and,membrane
R4310 T5183 T5184 advmod immediately,cross-linked
R4311 T5184 T5181 conj cross-linked,membrane
R4312 T5185 T5184 prep for,cross-linked
R4313 T5186 T5187 nummod 15,minutes
R4314 T5187 T5185 pobj minutes,for
R4315 T5188 T5187 prep with,minutes
R4316 T5189 T5191 det a,transilluminator
R4317 T5190 T5191 compound UV,transilluminator
R4318 T5191 T5188 pobj transilluminator,with
R4319 T5192 T5187 acl equipped,minutes
R4320 T5193 T5192 prep with,equipped
R4321 T5194 T5197 det a,bulb
R4322 T5195 T5197 nummod 312,bulb
R4323 T5196 T5197 compound nm,bulb
R4324 T5197 T5193 pobj bulb,with
R4325 T5198 T5168 punct .,transferred
R4326 T5199 T5201 amod Streptavidin-horseradish,conjugate
R4327 T5200 T5201 compound peroxidase,conjugate
R4328 T5201 T5208 nsubjpass conjugate,used
R4329 T5202 T5201 cc and,conjugate
R4330 T5203 T5206 det the,Substrate
R4331 T5204 T5206 compound LightShift,Substrate
R4332 T5205 T5206 compound Chemiluminescent,Substrate
R4333 T5206 T5201 conj Substrate,conjugate
R4334 T5207 T5208 auxpass were,used
R4335 T5208 T5208 ROOT used,used
R4336 T5209 T5210 aux to,detect
R4337 T5210 T5208 xcomp detect,used
R4338 T5211 T5212 det the,biotin
R4339 T5212 T5210 dobj biotin,detect
R4340 T5213 T5214 compound end-labeled,DNA
R4341 T5214 T5208 npadvmod DNA,used
R4342 T5215 T5208 punct .,used
R4343 T5216 T5218 det The,membranes
R4344 T5217 T5218 compound nylon,membranes
R4345 T5218 T5220 nsubjpass membranes,exposed
R4346 T5219 T5220 auxpass were,exposed
R4347 T5220 T5220 ROOT exposed,exposed
R4348 T5221 T5222 aux to,x-ray
R4349 T5222 T5220 advcl x-ray,exposed
R4350 T5223 T5222 dobj film,x-ray
R4351 T5224 T5222 prep for,x-ray
R4352 T5225 T5226 quantmod 1,3
R4353 T5226 T5227 nummod 3,minutes
R4354 T5227 T5224 pobj minutes,for
R4355 T5228 T5227 prep for,minutes
R4356 T5229 T5228 pobj detection,for
R4357 T5230 T5220 punct .,exposed