bionlp-ost-19-BB-norm-train  

BB-norm@ldeleger:BB-norm-4105033 / 82-1469 JSONTXT

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Id Subject Object Predicate Lexical cue NCBI_Taxonomy OntoBiotope
T4 0-12 Habitat denotes Spheroplasts OBT:000061
T2 0-1387 Paragraph denotes Spheroplasts prepared by lysozyme treatment of cells of Pseudomonas aeruginosa, suspended in 20% sucrose or 0.2 m MgCl(2), were examined in detail. Preparation of spheroplasts in the presence of 0.2 m Mg(2+) released periplasmic alkaline phosphatase, whereas preparation in the presence of 20% sucrose did not, even though untreated cells released phosphatase when suspended in sucrose in the absence of lysozyme. Biochemical characterizations of the sucrose-lysozyme preparations indicated that lysozyme mediated a reassociation of the released phosphatase with the spheroplasts. In addition, the enzyme released from whole cells suspended in 20% sucrose (which represents 20 to 40% of the cell-bound phosphatase) reassociates with the cells in the presence of lysozyme. Electron microscopic examinations of various preparations revealed that phosphatase released in sucrose reassociated with the external cell wall layers in the presence of lysozyme, that sucrose-lysozyme prepared spheroplasts did not dissociate phosphatase which remained in the periplasm of sucrose-washed cells, and that phosphatase was never observed to be associated with the cytoplasmic membrane. A model to account for the binding of P. aeruginosa alkaline phosphatase to the internal portion of the tripartite layer of the cell wall rather than to the cytoplasmic membrane or peptidoglycan layer is presented.
T5 56-78 Microorganism denotes Pseudomonas aeruginosa 287
T6 93-104 Habitat denotes 20% sucrose OBT:000106
T7 108-121 Habitat denotes 0.2 m MgCl(2) OBT:000106
T8 163-175 Habitat denotes spheroplasts OBT:000061
T9 195-207 Habitat denotes 0.2 m Mg(2+) OBT:000106
T10 290-301 Habitat denotes 20% sucrose OBT:000106
T11 378-385 Habitat denotes sucrose OBT:000106
T12 451-480 Habitat denotes sucrose-lysozyme preparations OBT:000106
T13 567-579 Habitat denotes spheroplasts OBT:000061
T14 644-655 Habitat denotes 20% sucrose OBT:000106
T15 868-875 Habitat denotes sucrose OBT:000106
T16 958-974 Habitat denotes sucrose-lysozyme OBT:000106
T17 958-996 Habitat denotes sucrose-lysozyme prepared spheroplasts OBT:000061
T18 1063-1083 Habitat denotes sucrose-washed cells OBT:000061
T19 1211-1224 Microorganism denotes P. aeruginosa 287