bionlp-ost-19-BB-norm-train  

BB-norm@ldeleger:BB-norm-4105033 JSONTXT

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Id Subject Object Predicate Lexical cue NCBI_Taxonomy OntoBiotope
T1 0-81 Title denotes Interactions of alkaline phosphatase and the cell wall of Pseudomonas aeruginosa.
T3 58-80 Microorganism denotes Pseudomonas aeruginosa 287
T4 82-94 Habitat denotes Spheroplasts OBT:000061
T2 82-1469 Paragraph denotes Spheroplasts prepared by lysozyme treatment of cells of Pseudomonas aeruginosa, suspended in 20% sucrose or 0.2 m MgCl(2), were examined in detail. Preparation of spheroplasts in the presence of 0.2 m Mg(2+) released periplasmic alkaline phosphatase, whereas preparation in the presence of 20% sucrose did not, even though untreated cells released phosphatase when suspended in sucrose in the absence of lysozyme. Biochemical characterizations of the sucrose-lysozyme preparations indicated that lysozyme mediated a reassociation of the released phosphatase with the spheroplasts. In addition, the enzyme released from whole cells suspended in 20% sucrose (which represents 20 to 40% of the cell-bound phosphatase) reassociates with the cells in the presence of lysozyme. Electron microscopic examinations of various preparations revealed that phosphatase released in sucrose reassociated with the external cell wall layers in the presence of lysozyme, that sucrose-lysozyme prepared spheroplasts did not dissociate phosphatase which remained in the periplasm of sucrose-washed cells, and that phosphatase was never observed to be associated with the cytoplasmic membrane. A model to account for the binding of P. aeruginosa alkaline phosphatase to the internal portion of the tripartite layer of the cell wall rather than to the cytoplasmic membrane or peptidoglycan layer is presented.
T5 138-160 Microorganism denotes Pseudomonas aeruginosa 287
T6 175-186 Habitat denotes 20% sucrose OBT:000106
T7 190-203 Habitat denotes 0.2 m MgCl(2) OBT:000106
T8 245-257 Habitat denotes spheroplasts OBT:000061
T9 277-289 Habitat denotes 0.2 m Mg(2+) OBT:000106
T10 372-383 Habitat denotes 20% sucrose OBT:000106
T11 460-467 Habitat denotes sucrose OBT:000106
T12 533-562 Habitat denotes sucrose-lysozyme preparations OBT:000106
T13 649-661 Habitat denotes spheroplasts OBT:000061
T14 726-737 Habitat denotes 20% sucrose OBT:000106
T15 950-957 Habitat denotes sucrose OBT:000106
T16 1040-1056 Habitat denotes sucrose-lysozyme OBT:000106
T17 1040-1078 Habitat denotes sucrose-lysozyme prepared spheroplasts OBT:000061
T18 1145-1165 Habitat denotes sucrose-washed cells OBT:000061
T19 1293-1306 Microorganism denotes P. aeruginosa 287