bionlp-ost-19-BB-norm-train  

BB-norm@ldeleger:BB-norm-24831788 / 145-1457 JSONTXT

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Id Subject Object Predicate Lexical cue NCBI_Taxonomy OntoBiotope
T2 0-1312 Paragraph denotes Interpretation of high throughput screening (HTS) data in cell-based assays may be confounded by cytotoxic properties of screening compounds. Therefore, assessing cell toxicity in real time during the HTS process itself would be highly advantageous. Here, we investigate the potential of putatively impermeant, fluorescent, DNA-binding dyes to give cell toxicity readout during HTS. Amongst 19 DNA-binding dyes examined, three classes were identified that were (1) permeant, (2) cytotoxic, or (3) neither permeant nor cytotoxic during 3-day incubation with a macrophage cell line. In the last class, four dyes (SYTOX Green, CellTox Green, GelGreen, and EvaGreen) gave highly robust cytotoxicity data in 384-well screening plates. As proof of principle, successful combination with a luminescence-based assay in HTS format was demonstrated. Here, both intracellular growth of Legionella pneumophila (luminescence) and host cell viability (SYTOX Green exclusion) were assayed in the same screening well. Incorporation of membrane-impermeant, DNA-binding, fluorescent dyes in HTS assays should prove useful by allowing evaluation of cytotoxicity in real time, eliminating reagent addition steps and effort associated with endpoint cell viability analysis, and reducing the need for follow-up cytotoxicity screening.
T4 58-62 Habitat denotes cell OBT:000061
T5 163-167 Habitat denotes cell OBT:000061
T6 349-353 Habitat denotes cell OBT:000061
T7 559-579 Habitat denotes macrophage cell line OBT:000062|OBT:002995
T8 703-728 Habitat denotes 384-well screening plates OBT:000103
T9 875-897 Microorganism denotes Legionella pneumophila 446
T10 917-926 Habitat denotes host cell OBT:002995
T11 1228-1232 Habitat denotes cell OBT:002995