BB-kb@ldeleger:BB-kb-18346136 / 227-1229
Annnotations
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| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T3 | 0-1002 | Paragraph | denotes | The method consisted of a novel two-step enrichment involving overnight incubation in buffered peptone water and a 5-h subculture in Rappaport-Vassiliadis medium, lysis of bacterial cells and a Salmonella-specific 5'-nuclease real-time PCR with an exogenous internal amplification control. Because a two-step enrichment was used, the detection limit for dead S. enterica cells in artificially contaminated ice cream and salami samples was high at 10(7 )CFU (25 g)(-1), eliminating potential false-positive results. When the method was evaluated with a range of 100 naturally contaminated food samples, three positive samples were detected by both the real-time PCR-based method and by the standard microbiological method, according to EN ISO 6579. When the real-time PCR-based method was evaluated alongside the standard microbiological method according to EN ISO 6579 with 36 food samples artificially contaminated at a level of 10(0 )CFU (25 g)(-1), identical results were obtained from both methods. |
| T10 | 86-108 | Habitat | denotes | buffered peptone water |
| T11 | 133-161 | Habitat | denotes | Rappaport-Vassiliadis medium |
| T12 | 194-204 | Microorganism | denotes | Salmonella |
| T13 | 359-370 | Microorganism | denotes | S. enterica |
| T15-0 | 380-405 | _FRAGMENT | denotes | artificially contaminated |
| T14 | 380-415 | Habitat | denotes | artificially contaminated ice cream |
| T15-1 | 420-434 | Habitat | denotes | salami samples |
| T16 | 565-600 | Habitat | denotes | naturally contaminated food samples |
| T17 | 877-915 | Habitat | denotes | food samples artificially contaminated |
| C-T15-0 | T15-1 | T15-0 | _lexicallyChainedTo | salami samples,artificially contaminated |