PubMed_ArguminSci  

PubMed:25686916 JSONTXT 8 Projects

Annnotations TAB TSV DIC JSON TextAE

Id Subject Object Predicate Lexical cue
T1 112-204 DRI_Challenge denotes Gliotoxic responses complicate human eye diseases, the causes of which often remain obscure.
T2 205-249 DRI_Approach denotes Here, we activated Müller cells (MCs) by the
T3 278-420 DRI_Approach denotes (AAA) and assayed possible protective effects by pigment epithelium-derived factor (PEDF) in RPE-free retinal explants of the E6 chick embryo.
T4 421-570 DRI_Background denotes These models are suited to analyze gliotoxic reactions in vitro, since the avian retina contains only Müller cells (MCs) as glial components, and the
T5 589-623 DRI_Background denotes are devoid of a major PEDF source.
T6 624-783 DRI_Background denotes ChAT- and AChE-immunohistochemistry (IHC) revealed that AAA treatment disrupted the differentiation of cholinergic amacrine cells in the inner plexiform layer.
T7 784-915 DRI_Background denotes At the applied concentration of 1 mM AAA, apoptosis of MCs was slightly increased, as shown by TUNEL and caspase-3 activity assays.
T8 916-1070 DRI_Background denotes Concomitantly, cell-free gaps emerged in the middle of the retina, where MCs were swollen and amassed glutamine synthetase (shown by GS and Vimentin IHC).
T9 1071-1186 DRI_Background denotes AAA treatment strongly activated MCs, as shown by GFAP IHC, and by an increase of stress-related catalase activity.
T10 1187-1277 DRI_Outcome denotes Remarkably, nearly all effects of AAA on MCs were effectively counter-balanced by 50 ng/ml
T11 1295-1321 DRI_Outcome denotes , as also shown by RT-PCR.
T12 1322-1425 DRI_Approach denotes These findings suggest that supplementation with PEDF can protect the retina against gliotoxic attacks.
T13 1426-1516 DRI_Approach denotes Further studies should establish whether PEDF similarly protects a gliotoxic human retina.