PubMed_ArguminSci  

PubMed:25286189 JSONTXT 13 Projects

Annnotations TAB TSV DIC JSON TextAE

Id Subject Object Predicate Lexical cue
T1 128-267 DRI_Background denotes During spermatogenesis in mammals and in Drosophila melanogaster, male germ cells develop in a series of essential developmental processes.
T2 268-362 DRI_Approach denotes This includes differentiation from a stem cell population, mitotic amplification, and meiosis.
T3 363-514 DRI_Background denotes In addition, post-meiotic germ cells undergo a dramatic morphological reshaping process as well as a global epigenetic reconfiguration of the germ line
T4 550-557 DRI_Background denotes switch.
T5 558-772 DRI_Background denotes Studying the role of a protein in post-meiotic spermatogenesis using mutagenesis or other genetic tools is often impeded by essential embryonic, pre-meiotic, or meiotic functions of the protein under investigation.
T6 773-948 DRI_Challenge denotes The post-meiotic phenotype of a mutant of such a protein could be obscured through an earlier developmental block, or the interpretation of the phenotype could be complicated.
T7 949-1141 DRI_Challenge denotes The model organism Drosophila melanogaster offers a bypass to this problem: intact testes and even cysts of germ cells dissected from early pupae are able to develop ex vivo in culture medium.
T8 1142-1251 DRI_Background denotes Making use of such cultures allows microscopic imaging of living germ cells in testes and of germ-line cysts.
T9 1252-1465 DRI_Background denotes Importantly, the cultivated testes and germ cells also become accessible to pharmacological inhibitors, thereby permitting manipulation of enzymatic functions during spermatogenesis, including post-meiotic stages.
T10 1466-1561 DRI_Outcome denotes The protocol presented describes how to dissect and cultivate pupal testes and germ-line cysts.
T11 1562-1725 DRI_Background denotes Information on the development of pupal testes and culture conditions are provided alongside microscope imaging data of live testes and germ-line cysts in culture.
T12 1726-1933 DRI_Background denotes We also describe a pharmacological assay to study post-meiotic spermatogenesis, exemplified by an assay targeting the histone-to-protamine switch using the histone acetyltransferase inhibitor anacardic acid.
T13 1934-2071 DRI_Background denotes In principle, this cultivation method could be adapted to address many other research questions in pre- and post-meiotic spermatogenesis.