PubMed_ArguminSci  

PubMed:20489151 JSONTXT 8 Projects

Annnotations TAB TSV DIC JSON TextAE

Id Subject Object Predicate Lexical cue
T1 107-252 DRI_Background denotes Calcified aortic valve (AV) cusps have increased expression of bone morphogenic proteins (BMPs) and transforming growth factor-beta1 (TGF-beta1).
T2 253-379 DRI_Approach denotes Elevated stretch loading on the AV is known to increase expression of matrix remodeling enzymes and pro-inflammatory proteins.
T3 380-482 DRI_Background denotes Little, however, is known about the mechanism by which elevated stretch might induce AV calcification.
T4 483-596 DRI_Approach denotes We investigated the hypothesis that elevated stretch may cause valve calcification via a BMP-dependent mechanism.
T5 597-911 DRI_Outcome denotes Porcine AV cusps were cultured in a stretch bioreactor, at 10% (physiological) or 15% (pathological) stretch and 70 beats per minute for 3, 7, and 14 days, in osteogenic media supplemented with or without high phosphate (3.8 mmol/L), TGF-beta1 (1 ng/ml), as well as the BMP inhibitor noggin (1, 10, and 100 ng/ml).
T6 912-943 DRI_Approach denotes Fresh cusps served as controls.
T7 944-1148 DRI_Outcome denotes Alizarin red and von Kossa staining demonstrated that 15% stretch elicited a stronger calcification response compared with 10% stretch in a fully osteogenic medium containing high phosphate and TGF-beta1.
T8 1149-1284 DRI_Background denotes BMP-2, -4, and Runx2 expression was observed after 3 days on the fibrosa surface of the valve cusp and was stretch magnitude-dependent.
T9 1285-1331 DRI_Background denotes Cellular apoptosis was highest at 15% stretch.
T10 1332-1490 DRI_Background denotes Tissue calcium content and alkaline phosphatase activity were similarly stretch-dependent and were significantly reduced by noggin in a dose dependent manner.
T11 1491-1588 DRI_Challenge denotes These results underline the potential role of BMPs in valve calcification due to altered stretch.