| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T77 |
0-6 |
Sentence |
denotes |
4.1.2. |
| T78 |
8-51 |
Sentence |
denotes |
Interference of the membrane fusion process |
| T79 |
52-179 |
Sentence |
denotes |
CoVs are enveloped RNA viruses, and their cell entry processes involve a principal route of receptor-mediated endocytosis [28]. |
| T80 |
180-344 |
Sentence |
denotes |
Membrane fusion takes place in the endosomal compartment after endocytosis, which needs additional triggers such as pH acidification or proteolytic activation [29]. |
| T81 |
345-584 |
Sentence |
denotes |
Multiple cellular proteases, such as trypsin, furin, proprotein convertase (PC) family, cathepsins, transmembrane protease/serine (TMPRSS) proteases and elastase, are involved in S protein activation, which can induce membrane fusion [30]. |
| T82 |
585-819 |
Sentence |
denotes |
Among them, cathepsin L, with anoptimal pH of 3.0 to 6.5, is most commonly associated with activation of a variety of CoV S proteins [30], such as SARS-CoV [19], MERS-CoV [31], HCoV-229E [32], and mouse hepatitis virus 2 (MHV-2) [33]. |
| T83 |
820-982 |
Sentence |
denotes |
A recent study found that SARS-CoV-2 enters 293/hACE2 cells mainly through endocytosis, in which cathepsin L is critical for priming of SARS-CoV-2 S protein [24]. |
| T84 |
983-1332 |
Sentence |
denotes |
A study investigated the detailed mechanism of action of CQ/HCQ in inhibiting SARS-CoV-2 entry, and co-localization of SARS-CoV-2 with early endosomes (EEs) or endolysosomes (ELs) in VeroE6 cells, and the results showed that CQ/HCQ hampered the transport of SARS-CoV-2 from EEs to ELs, indicating that CQ/HCQ might inhibit endosomal maturation [17]. |
| T85 |
1333-1596 |
Sentence |
denotes |
These studies revealed that the mechanism of anti-CoV activity of CQ/HCQ may involve the inhibition of the endosome acidification process, which might inactivate lysosomal proteases, thus interfering with the fusion of virus and host membranes [34,35] (Figure 1). |
