| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T46 |
0-109 |
Sentence |
denotes |
However, in cases where there are two basic centers in the molecule, this effect is considerably potentiated. |
| T47 |
110-294 |
Sentence |
denotes |
There are two different monobasic species that are produced by protonation of the respective nitrogens, and neither of these can easily diffuse from the lysosome back into the cytosol. |
| T48 |
295-409 |
Sentence |
denotes |
Furthermore, both species are in equilibrium with the biprotonated species, which is also trapped in the lysosome. |
| T49 |
410-558 |
Sentence |
denotes |
Therefore, there are three different forms of the molecule (two monoprotonated and one biprotonated) that cannot easily diffuse back to the cytosol. |
| T50 |
559-611 |
Sentence |
denotes |
This tremendously magnifies the ion-trapping effect. |
| T51 |
612-900 |
Sentence |
denotes |
The expected accumulation ratio under these conditions is calculated as follows:(2) R=C1C2=H12+Ka1*H1+Ka1*Ka2H22+Ka1*H2+Ka1*Ka2where H1 and H2 are the respective proton concentrations (=10−pH) of the two environments (pH 5 and 7.4) and Ka1 and Ka2 are the dissociation constant (=10−pKa). |
| T52 |
901-1047 |
Sentence |
denotes |
Fig. 3B shows the magnitude of the resulting accumulation as a function of the pKa value of the compound, assuming equal values for pKa1 and pKa2. |
| T53 |
1048-1165 |
Sentence |
denotes |
Accumulation of up to 60 000-fold higher concentrations in the lysosomes can be explained by the described mechanism. |
