{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/7199903","sourcedb":"PMC","sourceid":"7199903","source_url":"https://www.ncbi.nlm.nih.gov/pmc/7199903","text":"Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the causative pathogen of coronavirus 2019 (COVID-19) (1, 2), induces fever, severe respiratory illness, and pneumonia. SARS-CoV-2 uses an extensively glycosylated spike (S) protein that protrudes from the viral surface to bind to angiotensin-converting enzyme 2 (ACE2) to mediate host-cell entry (3). The S protein is a trimeric class I fusion protein, composed of two functional subunits, responsible for receptor binding (S1 subunit) and membrane fusion (S2 subunit) (4, 5). The surface of the envelope spike is dominated by host-derived glycans, with each trimer displaying 66 N-linked glycosylation sites. The S protein is a key target in vaccine design efforts (6), and understanding the glycosylation of recombinant viral spikes can reveal fundamental features of viral biology and guide vaccine design strategies (7, 8).","typesettings":[{"style":"italic","span":{"begin":117,"end":118}},{"style":"italic","span":{"begin":120,"end":121}},{"style":"italic","span":{"begin":360,"end":361}},{"style":"italic","span":{"begin":533,"end":534}},{"style":"italic","span":{"begin":536,"end":537}},{"style":"italic","span":{"begin":730,"end":731}},{"style":"italic","span":{"begin":884,"end":885}},{"style":"italic","span":{"begin":887,"end":888}}],"project":"LitCovid-sample-PD-HP","denotations":[{"id":"T1","span":{"begin":132,"end":137},"obj":"Phenotype"},{"id":"T2","span":{"begin":146,"end":165},"obj":"Phenotype"},{"id":"T3","span":{"begin":171,"end":180},"obj":"Phenotype"}],"attributes":[{"id":"A1","pred":"hp_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/HP_0001945"},{"id":"A2","pred":"hp_id","subj":"T2","obj":"http://purl.obolibrary.org/obo/HP_0002086"},{"id":"A3","pred":"hp_id","subj":"T3","obj":"http://purl.obolibrary.org/obo/HP_0002090"}]}